FvH4_7g22080

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009016
Physical Location & Seq
Reverse (-)
17500726 .. 17503963
3238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22080.t1

Sequence Viewer

Length: 510 bp
ATGGACGCGCCTGCCTCCCAAGCCAGTCTCCTCCTCCAAAAACAACTCAAAGATCTTTGCAAGCACCCAGTTGATGGATTCTCGGCTGGTTTGATCGACGAGGGTAATATCTTTGAATGGAATGTCACCATTATTGGGCCCCCTGATACTCTCTATGATGGTGGTTTCTTCAATGCCATCATGAGCTTCCCATCTGATTACCCAAACAACCCTCCTACAGTGAGGTTTACCTCAGAGATATGGCATCCTAATGTTTACTCGGATGGAAAGGTTTGCATTTCAATTCTTCATCCCCCCGGTGATGATCCTAATGGCTACGAGCTTGCAAGCGAGCGGTGGAATCCAGTCCATACAGTTGAGAGCATTATGTTGAGTATAATATCAATGCTTTCAAGCCCTAATGATGAGTCTCCTGCAAATGTTGAAGCTGCGAAACAATGGAGAGAAGATAGAGACGGATTCAGGAAGAGAGTAGGTCGCTGCGTGAGGAAATCACAAGAAATGCTATAA

Protein Analysis

170

Amino Acids

18.89

Weight (kDa)

4.89

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 12 - 161 1.3e-50 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 334
AccII CGCG 1 cut(s) 8
AciI CCGC 1 cut(s) 334
AclWI GGATC 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 74, 135
AgsI TTSAA 5 cut(s) 116, 172, 282, 393, 425
AluBI AGCT 3 cut(s) 186, 322, 428
AluI AGCT 3 cut(s) 186, 322, 428
Alw26I GTCTC 3 cut(s) 32, 414, 447
AlwI GGATC 1 cut(s) 299
AoxI GGCC 1 cut(s) 137
ApaI GGGCCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 428, 480
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 2 cut(s) 137, 138
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 118, 311
BaeGI GKGCMC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 415, 467
BccI CCATC 5 cut(s) 68, 152, 185, 199, 257
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 32, 414, 447
BfmI CTRYAG 1 cut(s) 216
BglII AGATCT 1 cut(s) 52
BisI GCNGC 2 cut(s) 429, 481
BlsI GCNGC 2 cut(s) 430, 482
Bme1390I CCNGG 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 137, 138
BmiI GGNNCC 2 cut(s) 139, 140
BmrFI CCNGG 1 cut(s) 297
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 295
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 135
Bse1I ACTGG 3 cut(s) 24, 68, 344
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 3 cut(s) 244, 268, 289
BseLI CCNNNNNNNGG 2 cut(s) 74, 135
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 3 cut(s) 24, 68, 344
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 2 cut(s) 415, 467
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 297
BslI CCNNNNNNNGG 2 cut(s) 74, 135
BsmAI GTCTC 3 cut(s) 32, 414, 447
BsmBI CGTCTC 1 cut(s) 447
BsnI GGCC 1 cut(s) 139
Bsp120I GGGCCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 3 cut(s) 52, 93, 304
BspACI CCGC 1 cut(s) 334
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 1 cut(s) 245
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 139, 140
BspPI GGATC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 334
BsrI ACTGG 3 cut(s) 24, 68, 344
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 3 cut(s) 52, 93, 304
Bst4CI ACNGT 2 cut(s) 220, 355
Bst6I CTCTTC 1 cut(s) 461
BstC8I GCNNGC 5 cut(s) 12, 62, 324, 328, 332
BstDEI CTNAG 1 cut(s) 232
BstF5I GGATG 3 cut(s) 244, 268, 289
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 3 cut(s) 55, 96, 307
BstMAI GTCTC 3 cut(s) 32, 414, 447
BstMBI GATC 3 cut(s) 52, 93, 304
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 415, 467
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 1 cut(s) 139
BtsCI GGATG 3 cut(s) 244, 268, 289
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 5 cut(s) 12, 62, 324, 328, 332
CciI TCATGA 1 cut(s) 180
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 2 cut(s) 137, 138
CseI GACGC 1 cut(s) 14
CviAII CATG 1 cut(s) 181
CviJI RGCY 8 cut(s) 23, 86, 139, 186, 315, 322, 396, 428
CviKI_1 RGCY 8 cut(s) 23, 86, 139, 186, 315, 322, 396, 428
DdeI CTNAG 1 cut(s) 232
DpnI GATC 3 cut(s) 54, 95, 306
DpnII GATC 3 cut(s) 52, 93, 304
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 1 cut(s) 184
FaiI YATR 7 cut(s) 156, 182, 241, 351, 368, 377, 508
FatI CATG 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 429, 481
FokI GGATG 3 cut(s) 231, 275, 276
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 429, 481
GlaI GCGC 1 cut(s) 9
GluI GCNGC 2 cut(s) 429, 481
HaeIII GGCC 1 cut(s) 139
HapII CCGG 1 cut(s) 297
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 10
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 4 cut(s) 78, 340, 407, 459
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 118, 311
Hpy166II GTNNAC 2 cut(s) 228, 256
Hpy188I TCNGA 3 cut(s) 196, 235, 262
Hpy188III TCNNGA 2 cut(s) 181, 463
Hpy8I GTNNAC 2 cut(s) 228, 256
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 2 cut(s) 220, 355
HpyCH4V TGCA 4 cut(s) 60, 276, 326, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 232
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 3 cut(s) 52, 93, 304
LpnPI CCDG 9 cut(s) 24, 37, 72, 81, 156, 310, 357, 426, 448
Lsp1109I GCAGC 2 cut(s) 415, 467
LweI GCATC 1 cut(s) 253
MaeIII GTNAC 1 cut(s) 124
MalI GATC 3 cut(s) 54, 95, 306
MbiI CCGCTC 1 cut(s) 334
MboI GATC 3 cut(s) 52, 93, 304
MboII GAAGA 4 cut(s) 160, 278, 458, 478
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 1 cut(s) 282
MlyI GAGTC 1 cut(s) 416
MnlI CCTC 8 cut(s) 25, 41, 44, 94, 216, 222, 241, 480
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 1 cut(s) 297
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 20
NciI CCSGG 1 cut(s) 297
NdeII GATC 3 cut(s) 52, 93, 304
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 2 cut(s) 139, 140
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 1 cut(s) 124
PagI TCATGA 1 cut(s) 180
PfeI GAWTC 3 cut(s) 78, 340, 459
PflMI CCANNNNNTGG 1 cut(s) 74
PkrI GCNGC 2 cut(s) 430, 482
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 139, 140
PspOMI GGGCCC 1 cut(s) 137
PspPI GGNCC 2 cut(s) 137, 138
PsuI RGATCY 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 249
SatI GCNGC 2 cut(s) 429, 481
Sau3AI GATC 3 cut(s) 52, 93, 304
Sau96I GGNCC 2 cut(s) 137, 138
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 141
SetI ASST 7 cut(s) 188, 227, 233, 273, 324, 430, 478
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 249
Sse9I AATT 1 cut(s) 282
SsiI CCGC 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 220, 355
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 282
TfiI GAWTC 3 cut(s) 78, 340, 459
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 428, 480
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 1 cut(s) 278
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.