Rroxscaffold_4G00289910

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009016
Physical Location & Seq
Forward (+)
10529067 .. 10531589
2523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289910.1

Sequence Viewer

Length: 510 bp
ATGGACGCGCCTCCATCCCAAGCCAGTCTCCTCCTCCAAAAACAACTCAAAGATCTTTGCAAGCACCCAGTTGATGGATTCTCGGCTGGTTTGATCGACGAGGCTAATATCTTCGAATGGAACGTCACGATTATTGGCCCCCCTGATACTCTCTATGATGGTGGTTTCTTCAATGCTATCATGAGCTTCCCTAACGAGTACCCTAATAACCCTCCTACAGTCAGGTTTACCTCAGAGATATGGCACCCTAATGTTTACTCGGATGGAAAGGTTTGTATTTCAATTCTTCATCCCCCGGGTGATGATCCTAATGGCTACGAGCTTGCGAGTGAGCGCTGGAATCCAGTCCATACAGTTGAGAGCATTGTGTTGAGTATAATATCAATGCTTTCAAGCCCTAATGATGAGTCTCCTGCAAATGTTGAAGCTGCGAAACAATGGAGAGAAGATAGAGACGGGTTCAGGAAGAGAGTAGGTCGCTGCGTGAGGAAGTCACAAGAAATGCTATAA

Protein Analysis

169

Amino Acids

18.94

Weight (kDa)

4.91

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 12 - 161 7.1e-51 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccB7I CCANNNNNTGG 1 cut(s) 74
AccII CGCG 1 cut(s) 8
AclWI GGATC 1 cut(s) 299
AfaI GTAC 1 cut(s) 200
AfeI AGCGCT 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 74
AgsI TTSAA 4 cut(s) 172, 282, 393, 425
AluBI AGCT 3 cut(s) 186, 322, 428
AluI AGCT 3 cut(s) 186, 322, 428
Alw26I GTCTC 3 cut(s) 32, 414, 447
AlwI GGATC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 335
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 428, 480
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 2 cut(s) 10, 336
AspS9I GGNCC 1 cut(s) 137
AsuC2I CCSGG 2 cut(s) 296, 297
AsuHPI GGTGA 1 cut(s) 311
AsuII TTCGAA 1 cut(s) 114
AvaI CYCGRG 1 cut(s) 295
BanI GGYRCC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 415, 467
BccI CCATC 4 cut(s) 22, 68, 152, 257
BcnI CCSGG 2 cut(s) 296, 297
BcoDI GTCTC 3 cut(s) 32, 414, 447
BfmI CTRYAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 337
BglII AGATCT 1 cut(s) 52
BisI GCNGC 2 cut(s) 429, 481
BlsI GCNGC 2 cut(s) 430, 482
Bme1390I CCNGG 2 cut(s) 296, 297
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 2 cut(s) 139, 245
BmrFI CCNGG 2 cut(s) 296, 297
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 114
BpuMI CCSGG 2 cut(s) 296, 297
BsaJI CCNNGG 2 cut(s) 294, 295
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse1I ACTGG 3 cut(s) 24, 68, 344
BseDI CCNNGG 2 cut(s) 294, 295
BseGI GGATG 3 cut(s) 14, 268, 289
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 3 cut(s) 24, 68, 344
BseRI GAGGAG 2 cut(s) 20, 23
BseXI GCAGC 2 cut(s) 415, 467
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 138
BshNI GGYRCC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 3 cut(s) 32, 414, 447
BsmBI CGTCTC 1 cut(s) 447
BsnI GGCC 1 cut(s) 138
BsoBI CYCGRG 1 cut(s) 295
Bsp119I TTCGAA 1 cut(s) 114
Bsp143I GATC 3 cut(s) 52, 93, 304
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 245
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 139, 245
BspPI GGATC 1 cut(s) 299
BspT104I TTCGAA 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 243
BsrI ACTGG 3 cut(s) 24, 68, 344
BssECI CCNNGG 2 cut(s) 294, 295
BssMI GATC 3 cut(s) 52, 93, 304
Bst4CI ACNGT 2 cut(s) 220, 355
Bst6I CTCTTC 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 62, 324
BstDEI CTNAG 1 cut(s) 232
BstF5I GGATG 3 cut(s) 14, 268, 289
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 2 cut(s) 10, 336
BstKTI GATC 3 cut(s) 55, 96, 307
BstMAI GTCTC 3 cut(s) 32, 414, 447
BstMBI GATC 3 cut(s) 52, 93, 304
BstSCI CCNGG 2 cut(s) 294, 295
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 415, 467
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 1 cut(s) 138
BtsCI GGATG 3 cut(s) 14, 268, 289
Cac8I GCNNGC 2 cut(s) 62, 324
CciI TCATGA 1 cut(s) 180
CfoI GCGC 2 cut(s) 10, 336
Cfr13I GGNCC 1 cut(s) 137
Cfr9I CCCGGG 1 cut(s) 295
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 199
CviAII CATG 1 cut(s) 181
CviJI RGCY 9 cut(s) 23, 86, 104, 138, 186, 315, 322, 396, 428
CviKI_1 RGCY 9 cut(s) 23, 86, 104, 138, 186, 315, 322, 396, 428
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 1 cut(s) 232
DpnI GATC 3 cut(s) 54, 95, 306
DpnII GATC 3 cut(s) 52, 93, 304
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
Eco47III AGCGCT 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 447
FaeI CATG 1 cut(s) 184
FaiI YATR 6 cut(s) 156, 182, 241, 351, 377, 508
FatI CATG 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 429, 481
FokI GGATG 2 cut(s) 275, 276
Fsp4HI GCNGC 2 cut(s) 429, 481
GlaI GCGC 2 cut(s) 9, 335
GluI GCNGC 2 cut(s) 429, 481
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 296
HgaI GACGC 1 cut(s) 14
HhaI GCGC 2 cut(s) 10, 336
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 2 cut(s) 8, 334
HinP1I GCGC 2 cut(s) 8, 334
HinfI GANTC 3 cut(s) 78, 340, 407
HpaII CCGG 1 cut(s) 296
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 228, 256
Hpy188I TCNGA 2 cut(s) 235, 262
Hpy188III TCNNGA 3 cut(s) 127, 181, 463
Hpy8I GTNNAC 2 cut(s) 228, 256
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 2 cut(s) 220, 355
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 60, 416
HpyF3I CTNAG 1 cut(s) 232
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 2 cut(s) 8, 334
Kzo9I GATC 3 cut(s) 52, 93, 304
Lsp1109I GCAGC 2 cut(s) 415, 467
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 124, 492
MalI GATC 3 cut(s) 54, 95, 306
MboI GATC 3 cut(s) 52, 93, 304
MboII GAAGA 5 cut(s) 103, 160, 278, 458, 478
MflI RGATCY 1 cut(s) 52
MluCI AATT 1 cut(s) 282
MlyI GAGTC 1 cut(s) 416
MnlI CCTC 7 cut(s) 21, 41, 44, 94, 222, 241, 480
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 296, 297
MvnI CGCG 1 cut(s) 8
NciI CCSGG 2 cut(s) 296, 297
NdeII GATC 3 cut(s) 52, 93, 304
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 2 cut(s) 139, 245
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 124, 492
NspV TTCGAA 1 cut(s) 114
PagI TCATGA 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 2 cut(s) 78, 340
PflMI CCANNNNNTGG 1 cut(s) 74
PkrI GCNGC 2 cut(s) 430, 482
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
PspN4I GGNNCC 2 cut(s) 139, 245
PspPI GGNCC 1 cut(s) 137
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 249
SatI GCNGC 2 cut(s) 429, 481
Sau3AI GATC 3 cut(s) 52, 93, 304
Sau96I GGNCC 1 cut(s) 137
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 2 cut(s) 296, 297
SetI ASST 8 cut(s) 126, 188, 227, 233, 273, 324, 430, 478
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 114
SmaI CCCGGG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 249
Sse9I AATT 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 294, 295
TaaI ACNGT 2 cut(s) 220, 355
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 96, 114
TasI AATT 1 cut(s) 282
TfiI GAWTC 2 cut(s) 78, 340
TseFI GTSAC 2 cut(s) 124, 492
TseI GCWGC 2 cut(s) 428, 480
Tsp45I GTSAC 2 cut(s) 124, 492
TspDTI ATGAA 1 cut(s) 278
TspMI CCCGGG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 74
XmaI CCCGGG 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.