MD07G1195500.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009016
Physical Location & Seq
Reverse (-)
27428591 .. 27432207
3617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1195500.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCTACGGCTCCTTCTTCCCAGGCCAGCCTCCTCCTCCAAAAGCAACTCAAAGATCTCTGCAAACACCCAGTTGATGGATTCTCGGCCGGTTTGGTGAACGAAGACAACATCTTCGAATGGAACGTCACGATTATCGGCCCACCCGACACTCTCTATGAGGGCGGTTTCTTTAATGCCATCATGAGTTTCCCAGAGGATTATCCGTGCAACCCTCCTATAGTGAGATTTACCTCTGAGATGTGGCACCCCAATGTTTACGCTGATGGAAAGGTTTGTGTTTCAATTCTTCATCCCCCTGGTGACGATCCAAATGGCTACGAGCTTGCAACTGAGCGCTGGAATCCAGTCCATACAGTTGAGAGCATTCTTTTGAGTATAATCTCAATGCTTTCAAGCCCTAATGATGAATCTCCTGCAAATGTCGATGCTGCGAAACAATGGAGAGAGAGTAGGGACGAGTTCAGGAAGAAAGTAGGGCGGTGTGTCAGAAAGTCTCAAGAAATGTTATGA

Protein Analysis

171

Amino Acids

19.05

Weight (kDa)

4.93

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 13 - 162 1.2e-51 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 77
AciI CCGC 2 cut(s) 165, 481
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 87
AfeI AGCGCT 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 2 cut(s) 285, 396
AjnI CCWGG 2 cut(s) 21, 298
AluBI AGCT 1 cut(s) 325
AluI AGCT 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 1 cut(s) 302
Aor51HI AGCGCT 1 cut(s) 338
AoxI GGCC 3 cut(s) 24, 87, 139
ApeKI GCWGC 1 cut(s) 431
ArsI GACNNNNNNTTYG 4 cut(s) 98, 111, 130, 143
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 109, 314
AsuII TTCGAA 1 cut(s) 117
BanI GGYRCC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 418
BccI CCATC 3 cut(s) 71, 188, 260
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 23, 300
BcoDI GTCTC 1 cut(s) 501
BfmI CTRYAG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 340
BglII AGATCT 1 cut(s) 55
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 2 cut(s) 23, 300
BmgT120I GGNCC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 12, 248
BmrFI CCNGG 2 cut(s) 23, 300
BmrI ACTGGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 21, 298
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 71, 347
BseBI CCWGG 2 cut(s) 23, 300
BseDI CCNNGG 2 cut(s) 21, 298
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 228, 324
BseNI ACTGG 2 cut(s) 71, 347
BseRI GAGGAG 2 cut(s) 23, 26
BseX3I CGGCCG 1 cut(s) 87
BseXI GCAGC 1 cut(s) 418
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 26, 89, 141
BshNI GGYRCC 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 90
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 3 cut(s) 26, 89, 141
Bsp119I TTCGAA 1 cut(s) 117
Bsp143I GATC 2 cut(s) 55, 307
BspACI CCGC 2 cut(s) 165, 481
BspANI GGCC 3 cut(s) 26, 89, 141
BspCNI CTCAG 2 cut(s) 229, 325
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 12, 248
BspPI GGATC 1 cut(s) 302
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 71, 347
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 21, 298
BssMI GATC 2 cut(s) 55, 307
Bst2UI CCWGG 2 cut(s) 23, 300
Bst4CI ACNGT 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 28, 327
BstDEI CTNAG 2 cut(s) 237, 333
BstF5I GGATG 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 340
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 2 cut(s) 58, 310
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 2 cut(s) 55, 307
BstMCI CGRYCG 1 cut(s) 90
BstNI CCWGG 2 cut(s) 23, 300
BstSCI CCNGG 2 cut(s) 21, 298
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 418
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BstZI CGGCCG 1 cut(s) 87
BsuRI GGCC 3 cut(s) 26, 89, 141
BtsCI GGATG 1 cut(s) 292
Cac8I GCNNGC 2 cut(s) 28, 327
CciI TCATGA 1 cut(s) 183
CfoI GCGC 1 cut(s) 339
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 140
CviAII CATG 1 cut(s) 184
CviJI RGCY 9 cut(s) 5, 11, 26, 30, 89, 141, 318, 325, 399
CviKI_1 RGCY 9 cut(s) 5, 11, 26, 30, 89, 141, 318, 325, 399
DdeI CTNAG 2 cut(s) 237, 333
DpnI GATC 2 cut(s) 57, 309
DpnII GATC 2 cut(s) 55, 307
EaeI YGGCCR 1 cut(s) 87
EagI CGGCCG 1 cut(s) 87
EclXI CGGCCG 1 cut(s) 87
Eco47III AGCGCT 1 cut(s) 338
Eco52I CGGCCG 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 21, 298
FaeI CATG 1 cut(s) 187
FaiI YATR 6 cut(s) 159, 185, 221, 354, 380, 511
FaqI GGGAC 1 cut(s) 470
FatI CATG 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 432
GlaI GCGC 1 cut(s) 338
GluI GCNGC 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 340
HaeIII GGCC 3 cut(s) 26, 89, 141
HapII CCGG 1 cut(s) 90
HhaI GCGC 1 cut(s) 339
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HinfI GANTC 3 cut(s) 81, 343, 410
HpaII CCGG 1 cut(s) 90
HphI GGTGA 2 cut(s) 109, 314
Hpy166II GTNNAC 2 cut(s) 100, 259
Hpy188I TCNGA 2 cut(s) 238, 491
Hpy188III TCNNGA 4 cut(s) 130, 184, 466, 500
Hpy8I GTNNAC 2 cut(s) 100, 259
HpyAV CCTTC 1 cut(s) 24
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 4 cut(s) 63, 210, 329, 419
HpyF3I CTNAG 2 cut(s) 237, 333
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 55, 307
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 418
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 127, 302
MalI GATC 2 cut(s) 57, 309
MboI GATC 2 cut(s) 55, 307
MboII GAAGA 5 cut(s) 9, 106, 116, 281, 481
MflI RGATCY 1 cut(s) 55
MluCI AATT 1 cut(s) 285
MnlI CCTC 7 cut(s) 41, 44, 47, 154, 190, 225, 244
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 252
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 23, 300
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 2 cut(s) 23, 300
NdeII GATC 2 cut(s) 55, 307
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 12, 248
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 2 cut(s) 127, 302
NspV TTCGAA 1 cut(s) 117
PagI TCATGA 1 cut(s) 183
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 144
PctI GAATGC 1 cut(s) 366
PfeI GAWTC 3 cut(s) 81, 343, 410
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 21, 298
PspGI CCWGG 2 cut(s) 21, 298
PspN4I GGNNCC 2 cut(s) 12, 248
PspPI GGNCC 1 cut(s) 140
PsuI RGATCY 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 2 cut(s) 55, 307
Sau96I GGNCC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 23, 300
SetI ASST 4 cut(s) 129, 236, 276, 327
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 498
SmoI CTYRAG 1 cut(s) 498
Sse9I AATT 1 cut(s) 285
SsiI CCGC 2 cut(s) 165, 481
StyD4I CCNGG 2 cut(s) 21, 298
TaaI ACNGT 1 cut(s) 358
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 117, 426
TasI AATT 1 cut(s) 285
TfiI GAWTC 3 cut(s) 81, 343, 410
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TseFI GTSAC 2 cut(s) 127, 302
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 2 cut(s) 127, 302
TspDTI ATGAA 2 cut(s) 281, 423
TspGWI ACGGA 1 cut(s) 195
Van91I CCANNNNNTGG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.