FvH4_7g22600

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010937
Physical Location & Seq
Forward (+)
17808948 .. 17812386
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22600.t1

Sequence Viewer

Length: 1245 bp
ATGATGACAGCCACATCAACCGCGCTAATCCAGCGCGTGTGCTCCGTCCCCCTCGCGCGTGCCGTCCGCACTCGCGCCTTGGCCACCGCCGTGAACCTCTCCACCACTCCCACTCCACGCGCCTCCCCGGCCGACTCCTCGCGCGTCCTGCTCGGCTTGTCCGAAGCGGAGCTCCAGCAGCTCGCTCTCGACTTCAGTCAGCAAGGCTACAGAGGCAAGCAGCTTCATCAGCTTATTTACAAAAGAAAGATTAAAGATATTCAGGATTTTAGTCAAGTGCCTCTGGCATTCAGGAATGAACTTGAGGAAGCTGGGTGGAAGGTTGGGAGGTCACCTATCTACAATACCGTCACCGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACCGGTTGATTGAAACTGTTGGCATACCGGTTCAAGATGAGAAAGGTTTGACGCGCCTTACAGCATGTGTCTCATCACAGGTCGGATGCCCGCTGCGTTGCTCCTTTTGTGCCACTGGGAAAGGAGGATTTTCAAGGAACCTTAAGAGGCATGAAATCGTTGAGCAGGTTTTGGCCATTGAGGAGATATTCAAGCATAGGGTGACTAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTCAAGTCTGTACTTGAAGCACACCAGTGCTTGAATAAGGATGTGCAAATTGGGCAAAGAATGATGACAATTTCTACGGTGGGGGTTCCAAATACAATTAAAAGGCTGGCATCTCACAAGCTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCAAACCAGAAACTTAGAGAAACAATTGTACCCAGTGCAAAAGCATATCCTCTAAGTGCACTTATGAAGGATTGCAGCGACTATTTCAATGAAACAAGTAGACGAGTTTCCTTTGAGTATGCCCTGTTAGCTGGAGTCAATGATGGAGTAGAGCATGCACGTGAACTTGCAGAGCTACTTCATCAGTGGGGAAAGGGTTATCATGTGAACTTGATACCTTTTAACCCAATAGAAGGTTCTGAGTATAAGCGTCCATCCAAGAGAGCTGTACAGGCATTTGCTGCTGCCCTGGAGTCCGGAAAAATAACTGTTAGCACACGTCAAACAAGGGGCCTTGATGCAGATGCAGCTTGTGGCCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTAGTCTCCGAGTCTGACAACTTACAACCTGAACCTGAAGCAGATGTTGCAGTTGCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

415

Amino Acids

45.53

Weight (kDa)

9.13

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 157 - 319 1.7e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 553
AccI GTMKAC 2 cut(s) 770, 895
AccII CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
AccIII TCCGGA 1 cut(s) 1091
AciI CCGC 6 cut(s) 21, 67, 87, 167, 354, 488
AcoI YGGCCR 4 cut(s) 81, 129, 570, 1150
AcuI CTGAAG 3 cut(s) 178, 749, 1239
AcvI CACGTG 1 cut(s) 956
AfaI GTAC 3 cut(s) 654, 825, 1065
AfiI CCNNNNNNNGG 1 cut(s) 1028
AflII CTTAAG 1 cut(s) 539
AflIII ACRYGT 1 cut(s) 1112
AgeI ACCGGT 2 cut(s) 399, 424
AgsI TTSAA 7 cut(s) 410, 431, 531, 589, 659, 676, 883
AjiI CACGTC 1 cut(s) 1115
AjnI CCWGG 1 cut(s) 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1015, 1047
AleI CACNNNNGTG 2 cut(s) 89, 667
Alw21I GWGCWC 3 cut(s) 44, 174, 856
Alw26I GTCTC 2 cut(s) 472, 1192
Alw44I GTGCAC 1 cut(s) 852
Aor13HI TCCGGA 1 cut(s) 1091
AoxI GGCC 5 cut(s) 81, 129, 570, 1126, 1150
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 8 cut(s) 178, 220, 356, 490, 870, 1076, 1079, 1142
AsiGI ACCGGT 2 cut(s) 399, 424
AspLEI GCGC 7 cut(s) 25, 36, 58, 77, 122, 144, 453
AspS9I GGNCC 1 cut(s) 1126
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 4 cut(s) 324, 343, 610, 638
BaeGI GKGCMC 1 cut(s) 856
BaeI ACNNNNGTAYC 2 cut(s) 807, 840
BalI TGGCCA 3 cut(s) 83, 572, 1152
BanII GRGCYC 1 cut(s) 174
BbrPI CACGTG 1 cut(s) 956
Bbv12I GWGCWC 3 cut(s) 44, 174, 856
BbvI GCAGC 8 cut(s) 190, 232, 343, 477, 882, 1063, 1066, 1154
BccI CCATC 3 cut(s) 356, 932, 1057
BceAI ACGGC 2 cut(s) 47, 74
BciT130I CCWGG 1 cut(s) 1085
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 472, 1192
BfmI CTRYAG 1 cut(s) 208
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 553
BglI GCCNNNNNGGC 1 cut(s) 128
BisI GCNGC 8 cut(s) 179, 221, 357, 491, 871, 1077, 1080, 1143
BlsI GCNGC 8 cut(s) 180, 222, 358, 492, 872, 1078, 1081, 1144
Bme1390I CCNGG 2 cut(s) 128, 1085
BmgBI CACGTC 1 cut(s) 1115
BmgT120I GGNCC 1 cut(s) 1126
BmiI GGNNCC 3 cut(s) 536, 729, 1127
BmrFI CCNGG 2 cut(s) 128, 1085
BmrI ACTGGG 2 cut(s) 522, 822
BmsI GCATC 4 cut(s) 473, 761, 1123, 1129
BmuI ACTGGG 2 cut(s) 522, 822
BoxI GACNNNNGTC 1 cut(s) 195
BpmI CTGGAG 3 cut(s) 158, 948, 1106
BpuEI CTTGAG 2 cut(s) 323, 629
BpuMI CCSGG 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 78, 126, 1083
BsaWI WCCGGW 3 cut(s) 399, 424, 1091
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 1028
Bse118I RCCGGY 2 cut(s) 399, 424
Bse1I ACTGG 3 cut(s) 517, 667, 828
BseAI TCCGGA 1 cut(s) 1091
BseBI CCWGG 1 cut(s) 1085
BseDI CCNNGG 3 cut(s) 78, 126, 1083
BseGI GGATG 3 cut(s) 488, 688, 1049
BseLI CCNNNNNNNGG 1 cut(s) 1028
BseMII CTCAG 1 cut(s) 1026
BseNI ACTGG 3 cut(s) 517, 667, 828
BseRI GAGGAG 2 cut(s) 127, 593
BseSI GKGCMC 1 cut(s) 856
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 8 cut(s) 190, 232, 343, 477, 882, 1063, 1066, 1154
BseYI CCCAGC 1 cut(s) 311
Bsh1236I CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 5 cut(s) 83, 131, 572, 1128, 1152
BshTI ACCGGT 2 cut(s) 399, 424
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 3 cut(s) 44, 174, 856
BsiSI CCGG 4 cut(s) 128, 400, 425, 1092
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 1028
BsmAI GTCTC 2 cut(s) 472, 1192
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 5 cut(s) 83, 131, 572, 1128, 1152
Bsp1286I GDGCHC 3 cut(s) 44, 174, 856
Bsp13I TCCGGA 1 cut(s) 1091
Bsp1407I TGTACA 1 cut(s) 1063
BspACI CCGC 6 cut(s) 21, 67, 87, 167, 354, 488
BspANI GGCC 5 cut(s) 83, 131, 572, 1128, 1152
BspCNI CTCAG 1 cut(s) 1027
BspEI TCCGGA 1 cut(s) 1091
BspFNI CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
BspLI GGNNCC 3 cut(s) 536, 729, 1127
BspMI ACCTGC 1 cut(s) 553
BspTI CTTAAG 1 cut(s) 539
BsrFI RCCGGY 2 cut(s) 399, 424
BsrGI TGTACA 1 cut(s) 1063
BsrI ACTGG 3 cut(s) 517, 667, 828
BssAI RCCGGY 2 cut(s) 399, 424
BssECI CCNNGG 3 cut(s) 78, 126, 1083
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 1085
Bst4CI ACNGT 5 cut(s) 349, 370, 415, 721, 1105
Bst6I CTCTTC 1 cut(s) 1169
BstAFI CTTAAG 1 cut(s) 539
BstAPI GCANNNNNTGC 2 cut(s) 1076, 1229
BstAUI TGTACA 1 cut(s) 1063
BstBAI YACGTR 1 cut(s) 956
BstC8I GCNNGC 9 cut(s) 60, 183, 218, 488, 750, 788, 792, 951, 1154
BstDEI CTNAG 4 cut(s) 809, 848, 1035, 1157
BstEII GGTNACC 1 cut(s) 330
BstF5I GGATG 3 cut(s) 488, 688, 1049
BstFNI CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
BstHHI GCGC 7 cut(s) 25, 36, 58, 77, 122, 144, 453
BstMAI GTCTC 2 cut(s) 472, 1192
BstMCI CGRYCG 1 cut(s) 132
BstNI CCWGG 1 cut(s) 1085
BstNSI RCATGY 4 cut(s) 465, 794, 953, 1242
BstPAI GACNNNNGTC 1 cut(s) 195
BstPI GGTNACC 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 126, 1083
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 856
BstUI CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
BstV1I GCAGC 8 cut(s) 190, 232, 343, 477, 882, 1063, 1066, 1154
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 5 cut(s) 83, 131, 572, 1128, 1152
BtrI CACGTC 1 cut(s) 1115
BtsCI GGATG 3 cut(s) 488, 688, 1049
BtsIMutI CAGTG 5 cut(s) 366, 510, 674, 835, 986
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 9 cut(s) 60, 183, 218, 488, 750, 788, 792, 951, 1154
CfoI GCGC 7 cut(s) 25, 36, 58, 77, 122, 144, 453
Cfr10I RCCGGY 2 cut(s) 399, 424
Cfr13I GGNCC 1 cut(s) 1126
CseI GACGC 3 cut(s) 133, 457, 1034
Csp6I GTAC 3 cut(s) 653, 824, 1064
CspAI ACCGGT 2 cut(s) 399, 424
CviAII CATG 7 cut(s) 462, 548, 616, 791, 950, 998, 1239
CviQI GTAC 3 cut(s) 653, 824, 1064
DdeI CTNAG 4 cut(s) 809, 848, 1035, 1157
EaeI YGGCCR 4 cut(s) 81, 129, 570, 1150
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 1169
EarI CTCTTC 1 cut(s) 1169
Ecl136II GAGCTC 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 174
Eco52I CGGCCG 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 3 cut(s) 178, 749, 1239
Eco72I CACGTG 1 cut(s) 956
Eco91I GGTNACC 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 1126
EcoO65I GGTNACC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 1083
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 7 cut(s) 465, 551, 619, 794, 953, 1001, 1242
FaqI GGGAC 1 cut(s) 32
FatI CATG 7 cut(s) 461, 547, 615, 790, 949, 997, 1238
FauI CCCGC 1 cut(s) 495
FblI GTMKAC 2 cut(s) 770, 895
Fnu4HI GCNGC 8 cut(s) 179, 221, 357, 491, 871, 1077, 1080, 1143
FokI GGATG 3 cut(s) 495, 695, 1036
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 8 cut(s) 179, 221, 357, 491, 871, 1077, 1080, 1143
GlaI GCGC 7 cut(s) 24, 35, 57, 76, 121, 143, 452
GluI GCNGC 8 cut(s) 179, 221, 357, 491, 871, 1077, 1080, 1143
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 3 cut(s) 158, 948, 1106
HaeIII GGCC 5 cut(s) 83, 131, 572, 1128, 1152
HapII CCGG 4 cut(s) 128, 400, 425, 1092
HgaI GACGC 3 cut(s) 133, 457, 1034
HhaI GCGC 7 cut(s) 25, 36, 58, 77, 122, 144, 453
Hin1II CATG 7 cut(s) 465, 551, 619, 794, 953, 1001, 1242
Hin6I GCGC 7 cut(s) 23, 34, 56, 75, 120, 142, 451
HinP1I GCGC 7 cut(s) 23, 34, 56, 75, 120, 142, 451
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 5 cut(s) 134, 930, 1088, 1177, 1193
HpaII CCGG 4 cut(s) 128, 400, 425, 1092
HphI GGTGA 4 cut(s) 324, 343, 610, 638
Hpy166II GTNNAC 7 cut(s) 94, 629, 771, 854, 896, 959, 1003
Hpy188I TCNGA 5 cut(s) 163, 482, 1036, 1192, 1198
Hpy188III TCNNGA 6 cut(s) 188, 263, 292, 431, 1092, 1171
Hpy8I GTNNAC 7 cut(s) 94, 629, 771, 854, 896, 959, 1003
HpyAV CCTTC 3 cut(s) 313, 856, 1022
HpyCH4III ACNGT 5 cut(s) 349, 370, 415, 721, 1105
HpyCH4IV ACGT 2 cut(s) 955, 1114
HpyF3I CTNAG 4 cut(s) 809, 848, 1035, 1157
HpySE526I ACGT 2 cut(s) 955, 1114
Hsp92II CATG 7 cut(s) 465, 551, 619, 794, 953, 1001, 1242
HspAI GCGC 7 cut(s) 23, 34, 56, 75, 120, 142, 451
Kpn2I TCCGGA 1 cut(s) 1091
LmnI GCTCC 5 cut(s) 47, 169, 177, 503, 799
Lsp1109I GCAGC 8 cut(s) 190, 232, 343, 477, 882, 1063, 1066, 1154
LweI GCATC 4 cut(s) 473, 761, 1123, 1129
MaeII ACGT 2 cut(s) 955, 1114
MaeIII GTNAC 3 cut(s) 330, 349, 598
MboII GAAGA 1 cut(s) 1186
MfeI CAATTG 1 cut(s) 819
MhlI GDGCHC 3 cut(s) 44, 174, 856
MlsI TGGCCA 3 cut(s) 83, 572, 1152
MluCI AATT 4 cut(s) 690, 711, 738, 819
MluNI TGGCCA 3 cut(s) 83, 572, 1152
MlyI GAGTC 5 cut(s) 128, 939, 1097, 1186, 1202
MmeI TCCRAC 2 cut(s) 369, 460
Mox20I TGGCCA 3 cut(s) 83, 572, 1152
MroI TCCGGA 1 cut(s) 1091
MscI TGGCCA 3 cut(s) 83, 572, 1152
MseI TTAA 5 cut(s) 252, 372, 540, 741, 1017
MslI CAYNNNNRTG 4 cut(s) 89, 620, 667, 954
Msp20I TGGCCA 3 cut(s) 83, 572, 1152
MspA1I CMGCKG 2 cut(s) 356, 490
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 4 cut(s) 128, 400, 425, 1092
MspR9I CCNGG 2 cut(s) 128, 1085
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 1085
MvnI CGCG 9 cut(s) 23, 36, 56, 58, 75, 120, 142, 144, 451
NciI CCSGG 1 cut(s) 128
NlaIII CATG 7 cut(s) 465, 551, 619, 794, 953, 1001, 1242
NlaIV GGNNCC 3 cut(s) 536, 729, 1127
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 3 cut(s) 330, 349, 598
NspI RCATGY 4 cut(s) 465, 794, 953, 1242
OliI CACNNNNGTG 2 cut(s) 89, 667
PaeI GCATGC 2 cut(s) 794, 953
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 159
PctI GAATGC 1 cut(s) 287
PinAI ACCGGT 2 cut(s) 399, 424
PkrI GCNGC 8 cut(s) 180, 222, 358, 492, 872, 1078, 1081, 1144
PleI GAGTC 5 cut(s) 128, 938, 1096, 1185, 1201
PmaCI CACGTG 1 cut(s) 956
PmlI CACGTG 1 cut(s) 956
PpsI GAGTC 5 cut(s) 128, 938, 1096, 1185, 1201
Ppu21I YACGTR 1 cut(s) 956
PshAI GACNNNNGTC 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 1083
PspCI CACGTG 1 cut(s) 956
PspEI GGTNACC 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 1083
PspN4I GGNNCC 3 cut(s) 536, 729, 1127
PspPI GGNCC 1 cut(s) 1126
RsaI GTAC 3 cut(s) 654, 825, 1065
RsaNI GTAC 3 cut(s) 653, 824, 1064
RseI CAYNNNNRTG 4 cut(s) 89, 620, 667, 954
SacI GAGCTC 1 cut(s) 174
SalI GTCGAC 1 cut(s) 769
SaqAI TTAA 5 cut(s) 252, 372, 540, 741, 1017
SatI GCNGC 8 cut(s) 179, 221, 357, 491, 871, 1077, 1080, 1143
Sau96I GGNCC 1 cut(s) 1126
SchI GAGTC 5 cut(s) 128, 939, 1097, 1186, 1202
ScrFI CCNGG 2 cut(s) 128, 1085
SduI GDGCHC 3 cut(s) 44, 174, 856
SfaNI GCATC 4 cut(s) 473, 761, 1123, 1129
SfcI CTRYAG 1 cut(s) 208
SmiMI CAYNNNNRTG 4 cut(s) 89, 620, 667, 954
SmlI CTYRAG 3 cut(s) 302, 539, 644
SmoI CTYRAG 3 cut(s) 302, 539, 644
SphI GCATGC 2 cut(s) 794, 953
Sse9I AATT 4 cut(s) 690, 711, 738, 819
SsiI CCGC 6 cut(s) 21, 67, 87, 167, 354, 488
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 2 cut(s) 126, 1083
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 5 cut(s) 349, 370, 415, 721, 1105
TaiI ACGT 2 cut(s) 958, 1117
TaqI TCGA 2 cut(s) 189, 770
TasI AATT 4 cut(s) 690, 711, 738, 819
TatI WGTACW 2 cut(s) 652, 1063
Tru1I TTAA 5 cut(s) 252, 372, 540, 741, 1017
Tru9I TTAA 5 cut(s) 252, 372, 540, 741, 1017
TscAI CASTG 5 cut(s) 373, 517, 674, 835, 986
TseFI GTSAC 3 cut(s) 330, 349, 598
TseI GCWGC 8 cut(s) 178, 220, 356, 490, 870, 1076, 1079, 1142
Tsp45I GTSAC 3 cut(s) 330, 349, 598
TspDTI ATGAA 8 cut(s) 215, 312, 564, 604, 653, 875, 900, 965
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 5 cut(s) 373, 517, 674, 835, 986
Vha464I CTTAAG 1 cut(s) 539
VneI GTGCAC 1 cut(s) 852
XceI RCATGY 4 cut(s) 465, 794, 953, 1242
XmiI GTMKAC 2 cut(s) 770, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.