MD07G1012700.v1.1

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010937
Physical Location & Seq
Reverse (-)
1136390 .. 1139389
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1012700.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGATGACAGCCTCGTCAACGTCGCTAATTCAGCGCGTCTGCGCCGTTCCTCTCTCACGTGCTGCCCGCACTCGCGCCATGGCAGCGGCGAGCATGAACCTGTCCACCACCACCACCGCCCCCAAAAGCACCGACCCCCAAGTCCTCCTCGGCTTGTCGGAACCAGAGCTCCAGCAGCTAGCTATCGACTTCAGTCAGCAAGGCTACAGAGGGAAGCAGCTCCATCATCTTATTTACAAAAGAAAGATTAAAGATATTCAGGATTTCAGTCAGGTGCCTCTGGCATTCAGAAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGTAGGTCACCCATTTACCAGAGCGTCACTGCTGCTGATGGCACCGTCAAGGTATTGATAAGGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGACGAGAAAGGTGTGATGCGCCTTACAGCGTGTGTCTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGTGCCACGGGAAAAGGAGGATTTTCAAGAAACCTCAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATAGAGGAAATCTTCAGCCATAGGGTGACTAATGTCGTGTTCATGGGAATGGGTGAACCGATGATGAACCTGAAGGTGGTACTTGAAGCGCATCAGTGTCTAAACAAGGATATTCAAATTGGGCAAAGAATGATCACAATTTCTACCGTGGGGGTTCCAAATACAATTAAAAGGCTGGCATCTCACAAACTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAGCTGAGGGAAACAATTGTGCCAAGTGCAAAAGCATACCCTCTGAGTGCACTGATGAGGGATTGCAGGGACTATTTCACTGAAACCGGTAGACGGGTTTCCTTTGAGTATGCACTCTTAGCTGGAGTTAATGATGCGGTAGACCATGCAGTAGAACTTGCAGAGCTACTCCATGAGTGGGGACGAGGTTCTCATGTGAACTTGATACCATTTAATCCAATAGAAGGCTCTGAGTATAAACGTCCATCCAAGAAAGCGGTACAAGCATTTGCAGCTGCTCTGGAGAACAACAAAGTAACGGTCAGCACTCGTCAAACAAGGGGCTTGGATGCAGATGCAGCATGTGGTCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGCCCGTTGACTCTGACAACTTACAAACTGAAAAAGATGCCGCAGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

414

Amino Acids

45.41

Weight (kDa)

9.14

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 156 - 320 3.8e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 13, 140
Acc36I ACCTGC 1 cut(s) 550
AccB1I GGYRCC 2 cut(s) 274, 362
AccI GTMKAC 3 cut(s) 767, 892, 942
AccII CGCG 2 cut(s) 36, 75
AciI CCGC 6 cut(s) 67, 86, 117, 938, 1058, 1221
AcuI CTGAAG 4 cut(s) 175, 568, 662, 746
AcvI CACGTG 1 cut(s) 59
AfaI GTAC 2 cut(s) 651, 1062
AfiI CCNNNNNNNGG 4 cut(s) 646, 894, 979, 1025
AgeI ACCGGT 1 cut(s) 887
AgsI TTSAA 4 cut(s) 407, 528, 656, 686
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
Alw21I GWGCWC 2 cut(s) 171, 853
Alw26I GTCTC 1 cut(s) 469
Alw44I GTGCAC 1 cut(s) 849
AlwNI CAGNNNCTG 1 cut(s) 805
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 8 cut(s) 62, 83, 175, 217, 353, 1073, 1076, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 1086, 1118
AsiGI ACCGGT 1 cut(s) 887
AspLEI GCGC 5 cut(s) 36, 44, 77, 450, 661
AsuHPI GGTGA 3 cut(s) 321, 607, 635
AsuNHI GCTAGC 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 853
BanI GGYRCC 2 cut(s) 274, 362
BanII GRGCYC 1 cut(s) 171
BbrPI CACGTG 1 cut(s) 59
Bbv12I GWGCWC 2 cut(s) 171, 853
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 8 cut(s) 49, 95, 187, 229, 340, 1063, 1085, 1151
BccI CCATC 4 cut(s) 231, 306, 353, 1054
BceAI ACGGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 702
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 2 cut(s) 205, 570
BfuAI ACCTGC 1 cut(s) 550
BmiI GGNNCC 4 cut(s) 162, 276, 364, 726
BmsI GCATC 8 cut(s) 435, 470, 670, 758, 925, 1120, 1126, 1207
BmtI GCTAGC 1 cut(s) 182
BoxI GACNNNNGTC 2 cut(s) 192, 602
BpmI CTGGAG 3 cut(s) 155, 945, 1103
Bpu10I CCTNAGC 1 cut(s) 806
BpuEI CTTGAG 2 cut(s) 320, 521
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 4 cut(s) 78, 148, 507, 717
BsaWI WCCGGW 1 cut(s) 887
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 4 cut(s) 646, 894, 979, 1025
Bse118I RCCGGY 1 cut(s) 887
Bse1I ACTGG 1 cut(s) 422
BseDI CCNNGG 4 cut(s) 78, 148, 507, 717
BseGI GGATG 4 cut(s) 317, 485, 1046, 1135
BseLI CCNNNNNNNGG 4 cut(s) 646, 894, 979, 1025
BseMII CTCAG 3 cut(s) 797, 836, 1023
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 8 cut(s) 49, 95, 187, 229, 340, 1063, 1085, 1151
Bsh1236I CGCG 2 cut(s) 36, 75
BshNI GGYRCC 2 cut(s) 274, 362
BshTI ACCGGT 1 cut(s) 887
BsiHKAI GWGCWC 2 cut(s) 171, 853
BsiSI CCGG 1 cut(s) 888
BslFI GGGAC 2 cut(s) 884, 996
BslI CCNNNNNNNGG 4 cut(s) 646, 894, 979, 1025
BsmAI GTCTC 1 cut(s) 469
BsmFI GGGAC 2 cut(s) 884, 996
BsmI GAATGC 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 171, 853
Bsp143I GATC 1 cut(s) 702
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 6 cut(s) 67, 86, 117, 938, 1058, 1221
BspCNI CTCAG 3 cut(s) 798, 837, 1024
BspFNI CGCG 2 cut(s) 36, 75
BspLI GGNNCC 4 cut(s) 162, 276, 364, 726
BspMI ACCTGC 1 cut(s) 550
BspOI GCTAGC 1 cut(s) 182
BspT107I GGYRCC 2 cut(s) 274, 362
BsrFI RCCGGY 1 cut(s) 887
BsrI ACTGG 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 887
BssECI CCNNGG 4 cut(s) 78, 148, 507, 717
BssMI GATC 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 4 cut(s) 367, 412, 718, 1102
Bst6I CTCTTC 2 cut(s) 492, 1166
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 6 cut(s) 67, 91, 180, 747, 785, 789
BstDEI CTNAG 5 cut(s) 806, 845, 919, 1032, 1154
BstDSI CCRYGG 3 cut(s) 78, 507, 717
BstEII GGTNACC 1 cut(s) 327
BstF5I GGATG 4 cut(s) 317, 485, 1046, 1135
BstFNI CGCG 2 cut(s) 36, 75
BstHHI GCGC 5 cut(s) 36, 44, 77, 450, 661
BstKTI GATC 1 cut(s) 705
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 1 cut(s) 702
BstMWI GCNNNNNNNGC 8 cut(s) 31, 83, 175, 920, 1064, 1073, 1139, 1226
BstNSI RCATGY 3 cut(s) 791, 1146, 1239
BstPAI GACNNNNGTC 2 cut(s) 192, 602
BstPI GGTNACC 1 cut(s) 327
BstSFI CTRYAG 2 cut(s) 205, 570
BstSLI GKGCMC 1 cut(s) 853
BstUI CGCG 2 cut(s) 36, 75
BstV1I GCAGC 8 cut(s) 49, 95, 187, 229, 340, 1063, 1085, 1151
BtgI CCRYGG 3 cut(s) 78, 507, 717
BtsCI GGATG 4 cut(s) 317, 485, 1046, 1135
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 4 cut(s) 348, 671, 851, 879
BveI ACCTGC 1 cut(s) 550
Cac8I GCNNGC 6 cut(s) 67, 91, 180, 747, 785, 789
CaiI CAGNNNCTG 1 cut(s) 805
CfoI GCGC 5 cut(s) 36, 44, 77, 450, 661
Cfr10I RCCGGY 1 cut(s) 887
CseI GACGC 2 cut(s) 25, 334
Csp6I GTAC 2 cut(s) 650, 1061
CspAI ACCGGT 1 cut(s) 887
CviQI GTAC 2 cut(s) 650, 1061
DdeI CTNAG 5 cut(s) 806, 845, 919, 1032, 1154
DpnI GATC 1 cut(s) 704
DpnII GATC 1 cut(s) 702
DrdI GACNNNNNNGTC 2 cut(s) 13, 140
DseDI GACNNNNNNGTC 2 cut(s) 13, 140
Eam1104I CTCTTC 2 cut(s) 492, 1166
EarI CTCTTC 2 cut(s) 492, 1166
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 171
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 4 cut(s) 175, 568, 662, 746
Eco72I CACGTG 1 cut(s) 59
Eco91I GGTNACC 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 169
EcoO65I GGTNACC 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 78
FaqI GGGAC 2 cut(s) 884, 996
FauI CCCGC 1 cut(s) 74
FbaI TGATCA 1 cut(s) 702
FblI GTMKAC 3 cut(s) 767, 892, 942
FokI GGATG 4 cut(s) 324, 492, 1033, 1142
FriOI GRGCYC 1 cut(s) 171
FspBI CTAG 1 cut(s) 179
GlaI GCGC 5 cut(s) 35, 43, 76, 449, 660
GsuI CTGGAG 3 cut(s) 155, 945, 1103
HapII CCGG 1 cut(s) 888
HgaI GACGC 2 cut(s) 25, 334
HhaI GCGC 5 cut(s) 36, 44, 77, 450, 661
Hin6I GCGC 5 cut(s) 34, 42, 75, 448, 659
HinP1I GCGC 5 cut(s) 34, 42, 75, 448, 659
HincII GTYRAC 3 cut(s) 18, 768, 1189
HindII GTYRAC 3 cut(s) 18, 768, 1189
HinfI GANTC 2 cut(s) 1174, 1190
HpaII CCGG 1 cut(s) 888
HphI GGTGA 3 cut(s) 321, 607, 635
Hpy166II GTNNAC 9 cut(s) 18, 105, 626, 768, 851, 893, 943, 1000, 1189
Hpy188I TCNGA 5 cut(s) 160, 290, 846, 1033, 1195
Hpy188III TCNNGA 5 cut(s) 260, 528, 538, 1082, 1168
Hpy8I GTNNAC 9 cut(s) 18, 105, 626, 768, 851, 893, 943, 1000, 1189
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 637, 1019
HpyCH4III ACNGT 4 cut(s) 367, 412, 718, 1102
HpyCH4IV ACGT 3 cut(s) 20, 58, 1042
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 83, 175, 920, 1064, 1073, 1139, 1226
HpyF3I CTNAG 5 cut(s) 806, 845, 919, 1032, 1154
HpySE526I ACGT 3 cut(s) 20, 58, 1042
HspAI GCGC 5 cut(s) 34, 42, 75, 448, 659
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 1 cut(s) 702
LmnI GCTCC 3 cut(s) 174, 225, 796
Lsp1109I GCAGC 8 cut(s) 49, 95, 187, 229, 340, 1063, 1085, 1151
LweI GCATC 8 cut(s) 435, 470, 670, 758, 925, 1120, 1126, 1207
MaeI CTAG 1 cut(s) 179
MaeII ACGT 3 cut(s) 20, 58, 1042
MaeIII GTNAC 4 cut(s) 327, 346, 595, 1096
MalI GATC 1 cut(s) 704
MboI GATC 1 cut(s) 702
MboII GAAGA 3 cut(s) 479, 574, 1183
MfeI CAATTG 1 cut(s) 816
MhlI GDGCHC 2 cut(s) 171, 853
MluCI AATT 6 cut(s) 27, 549, 687, 708, 735, 816
MlyI GAGTC 2 cut(s) 1183, 1184
MmeI TCCRAC 2 cut(s) 138, 366
MseI TTAA 5 cut(s) 249, 426, 738, 930, 1014
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 2 cut(s) 86, 1076
MspI CCGG 1 cut(s) 888
MunI CAATTG 1 cut(s) 816
Mva1269I GAATGC 1 cut(s) 284
MvnI CGCG 2 cut(s) 36, 75
MwoI GCNNNNNNNGC 8 cut(s) 31, 83, 175, 920, 1064, 1073, 1139, 1226
NcoI CCATGG 1 cut(s) 78
NdeII GATC 1 cut(s) 702
NheI GCTAGC 1 cut(s) 178
NlaIV GGNNCC 4 cut(s) 162, 276, 364, 726
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 3 cut(s) 327, 346, 595
NspI RCATGY 3 cut(s) 791, 1146, 1239
PaeI GCATGC 1 cut(s) 791
PctI GAATGC 1 cut(s) 284
PinAI ACCGGT 1 cut(s) 887
PleI GAGTC 2 cut(s) 1182, 1184
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 2 cut(s) 1182, 1184
Ppu21I YACGTR 1 cut(s) 59
PshAI GACNNNNGTC 2 cut(s) 192, 602
Psp124BI GAGCTC 1 cut(s) 171
PspCI CACGTG 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 327
PspN4I GGNNCC 4 cut(s) 162, 276, 364, 726
PstNI CAGNNNCTG 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 2 cut(s) 651, 1062
RsaNI GTAC 2 cut(s) 650, 1061
RseI CAYNNNNRTG 1 cut(s) 617
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 766
SaqAI TTAA 5 cut(s) 249, 426, 738, 930, 1014
Sau3AI GATC 1 cut(s) 702
SchI GAGTC 2 cut(s) 1183, 1184
SduI GDGCHC 2 cut(s) 171, 853
SfaNI GCATC 8 cut(s) 435, 470, 670, 758, 925, 1120, 1126, 1207
SfcI CTRYAG 2 cut(s) 205, 570
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 299, 536
SmoI CTYRAG 2 cut(s) 299, 536
SphI GCATGC 1 cut(s) 791
Sse9I AATT 6 cut(s) 27, 549, 687, 708, 735, 816
SsiI CCGC 6 cut(s) 67, 86, 117, 938, 1058, 1221
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 171
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 4 cut(s) 367, 412, 718, 1102
TaiI ACGT 3 cut(s) 23, 61, 1045
TaqI TCGA 2 cut(s) 186, 767
TasI AATT 6 cut(s) 27, 549, 687, 708, 735, 816
TauI GCSGC 2 cut(s) 89, 1223
Tru1I TTAA 5 cut(s) 249, 426, 738, 930, 1014
Tru9I TTAA 5 cut(s) 249, 426, 738, 930, 1014
TscAI CASTG 4 cut(s) 355, 671, 858, 886
TseFI GTSAC 3 cut(s) 327, 346, 595
TseI GCWGC 8 cut(s) 62, 83, 175, 217, 353, 1073, 1076, 1139
Tsp45I GTSAC 3 cut(s) 327, 346, 595
TspDTI ATGAA 4 cut(s) 110, 561, 601, 650
TspRI CASTG 4 cut(s) 355, 671, 858, 886
VneI GTGCAC 1 cut(s) 849
XceI RCATGY 3 cut(s) 791, 1146, 1239
XmiI GTMKAC 3 cut(s) 767, 892, 942
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.