RLG00000027269

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010937
Physical Location & Seq
Reverse (-)
10166115 .. 10169519
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027269

Sequence Viewer

Length: 1311 bp
ATGATGACAGCCACCTCAACGGCGCTAATCCAGCGCGTGTGCTCCATCCCCCTCGCGCGTGCCGTCCGTACTCGCGCCTTAGCCACCAACACCACCACCATGAGCCTCTCCACCACTCCCACTCCCACTCCCACTCGCGCCTCGCCGGCCGACTCGCGCGTCCTGCTCGGCTTGTCCGAACCGGAACTCCAGCAGCTCGCCCTCGACTTCAGTCAGCAAGGCTACAGAGGAAAGCAGCTTCATCATCTCATTTACAAAAGAAAGATTAAAGACATTCAGGATTTCAGTCAAGTGCCTCTGGCATTCAGGAATGAGCTTGAGGAAGCTGGATGGAAGGTTGGGAGGTCACCTATTTACAATACTGTTACTGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACCGATTGATTGAAACTGTTGGCATACCGGTTCAAGACGAGAAGGGTTTGATGCGCCTTACAGCATGTGTCTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGCGCTACAGGAAAAGGAGGGTTTTCAAGGAACCTTAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAGATATTCCAGCATAGGGTGACTAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTCAAGTCTGTACTTGAAGCACACCAGTGCTTGAATAAGGATGTGCAAATTGGGCAAAGAATGATGACAATTTCTACTGTGGGGGTTCCAAATACAATTAAAAGGCTTGCATCTCACAAGCTTCAGTCAACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAACTTAGGGAAACAATTGTACCAAGTGCGAAAGCATATCCTCTAAGTGCACTTATGAAGGATTGCAGCGACTATTTCAATGAAACAAATAGACGCGTTTCCTTTGAGTATGCCCTTTTAGCTGGAGTCAATGATGCAGTAGAGCACGCACGTGAACTTGCAGAGCTACTTCATCAGTGGGGACGAGGTTATCATGTGAACTTGATACCTTTTAATCCAATAGAAGGTTCTGAGTATAAGCGTCCATCCAAGAGAGCTGTCCAGGCATTTGCTGCTGCTCTGGAGTCCGGTAAAATAACTGTTAGCACACGTCAAACAAGGGGCCTTGATGCAGATGCAGCTTGTGGCCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGGTCTCCGAGTCTGACAACTTACAACCTGAACCTGAAGTAGATGTTGCAGTTGCATCAGTAGAAGCTCAACGGAAGTGGTGTTCAAAGTTGAAGATGACTAGCACCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

437

Amino Acids

48.24

Weight (kDa)

9.26

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 162 - 325 4.4e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
Acc36I ACCTGC 1 cut(s) 568
AccII CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
AcoI YGGCCR 2 cut(s) 147, 1165
AcuI CTGAAG 3 cut(s) 193, 764, 1254
AcvI CACGTG 1 cut(s) 971
AfaI GTAC 3 cut(s) 70, 669, 840
AfiI CCNNNNNNNGG 2 cut(s) 610, 1043
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 2 cut(s) 913, 1127
AgeI ACCGGT 1 cut(s) 439
AgsI TTSAA 8 cut(s) 425, 446, 546, 674, 691, 898, 1284, 1291
AjiI CACGTC 1 cut(s) 1130
AjnI CCWGG 1 cut(s) 1080
AjuI GAANNNNNNNTTGG 4 cut(s) 1030, 1062, 1181, 1213
AleI CACNNNNGTG 2 cut(s) 682, 969
AloI GAACNNNNNNTCC 4 cut(s) 171, 203, 1264, 1296
Alw21I GWGCWC 3 cut(s) 44, 871, 966
Alw26I GTCTC 2 cut(s) 487, 1207
Alw44I GTGCAC 1 cut(s) 867
AoxI GGCC 3 cut(s) 147, 1141, 1165
ApaLI GTGCAC 1 cut(s) 867
ApeKI GCWGC 7 cut(s) 193, 235, 371, 885, 1091, 1094, 1157
AsiGI ACCGGT 1 cut(s) 439
AspLEI GCGC 8 cut(s) 25, 36, 58, 77, 140, 159, 468, 524
AspS9I GGNCC 1 cut(s) 1141
AsuHPI GGTGA 3 cut(s) 339, 625, 653
BaeGI GKGCMC 1 cut(s) 871
BaeI ACNNNNGTAYC 2 cut(s) 822, 855
BalI TGGCCA 1 cut(s) 1167
BbrPI CACGTG 1 cut(s) 971
Bbv12I GWGCWC 3 cut(s) 44, 871, 966
BbvI GCAGC 7 cut(s) 205, 247, 358, 897, 1078, 1081, 1169
BccI CCATC 4 cut(s) 53, 324, 371, 1072
BceAI ACGGC 2 cut(s) 36, 47
BciT130I CCWGG 1 cut(s) 1082
BcoDI GTCTC 2 cut(s) 487, 1207
BfaI CTAG 2 cut(s) 1299, 1309
BfmI CTRYAG 2 cut(s) 223, 525
BfoI RGCGCY 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 568
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 7 cut(s) 194, 236, 372, 886, 1092, 1095, 1158
BlsI GCNGC 7 cut(s) 195, 237, 373, 887, 1093, 1096, 1159
Bme1390I CCNGG 1 cut(s) 1082
BmgBI CACGTC 1 cut(s) 1130
BmgT120I GGNCC 1 cut(s) 1141
BmiI GGNNCC 3 cut(s) 551, 744, 1142
BmrFI CCNGG 1 cut(s) 1082
BmsI GCATC 7 cut(s) 453, 488, 776, 943, 1138, 1144, 1262
BoxI GACNNNNGTC 1 cut(s) 210
BpmI CTGGAG 3 cut(s) 173, 963, 1121
Bpu10I CCTNAGC 1 cut(s) 79
BpuEI CTTGAG 2 cut(s) 338, 644
BsaAI YACGTR 1 cut(s) 971
BsaI GGTCTC 1 cut(s) 1207
BsaWI WCCGGW 3 cut(s) 181, 439, 1106
BsaXI ACNNNNNCTCC 6 cut(s) 106, 112, 136, 142, 171, 201
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 1043
Bse118I RCCGGY 2 cut(s) 145, 439
Bse1I ACTGG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 1082
BseGI GGATG 5 cut(s) 45, 335, 503, 703, 1064
BseLI CCNNNNNNNGG 2 cut(s) 610, 1043
BseMII CTCAG 1 cut(s) 1041
BseNI ACTGG 1 cut(s) 682
BseRI GAGGAG 1 cut(s) 608
BseSI GKGCMC 1 cut(s) 871
BseX3I CGGCCG 1 cut(s) 147
BseXI GCAGC 7 cut(s) 205, 247, 358, 897, 1078, 1081, 1169
Bsh1236I CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 149, 1143, 1167
BshTI ACCGGT 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 150
BsiHKAI GWGCWC 3 cut(s) 44, 871, 966
BsiSI CCGG 4 cut(s) 146, 182, 440, 1107
BslFI GGGAC 1 cut(s) 1014
BslI CCNNNNNNNGG 2 cut(s) 610, 1043
BsmAI GTCTC 2 cut(s) 487, 1207
BsmFI GGGAC 1 cut(s) 1014
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 3 cut(s) 149, 1143, 1167
Bso31I GGTCTC 1 cut(s) 1207
Bsp1286I GDGCHC 3 cut(s) 44, 871, 966
BspANI GGCC 3 cut(s) 149, 1143, 1167
BspCNI CTCAG 1 cut(s) 1042
BspFNI CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
BspLI GGNNCC 3 cut(s) 551, 744, 1142
BspMI ACCTGC 1 cut(s) 568
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 1207
BsrFI RCCGGY 2 cut(s) 145, 439
BsrI ACTGG 1 cut(s) 682
BssAI RCCGGY 2 cut(s) 145, 439
Bst2UI CCWGG 1 cut(s) 1082
Bst4CI ACNGT 5 cut(s) 364, 385, 430, 736, 1120
Bst6I CTCTTC 2 cut(s) 510, 1184
BstAFI CTTAAG 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 1091
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 8 cut(s) 60, 147, 198, 765, 803, 807, 966, 1169
BstDEI CTNAG 5 cut(s) 79, 824, 863, 1050, 1172
BstEII GGTNACC 1 cut(s) 345
BstF5I GGATG 5 cut(s) 45, 335, 503, 703, 1064
BstFNI CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 8 cut(s) 25, 36, 58, 77, 140, 159, 468, 524
BstMAI GTCTC 2 cut(s) 487, 1207
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 9 cut(s) 31, 146, 163, 519, 709, 938, 1082, 1091, 1157
BstNI CCWGG 1 cut(s) 1082
BstNSI RCATGY 2 cut(s) 480, 809
BstPAI GACNNNNGTC 1 cut(s) 210
BstPI GGTNACC 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 1080
BstSFI CTRYAG 2 cut(s) 223, 525
BstSLI GKGCMC 1 cut(s) 871
BstUI CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
BstV1I GCAGC 7 cut(s) 205, 247, 358, 897, 1078, 1081, 1169
BstZI CGGCCG 1 cut(s) 147
BsuRI GGCC 3 cut(s) 149, 1143, 1167
BtrI CACGTC 1 cut(s) 1130
BtsCI GGATG 5 cut(s) 45, 335, 503, 703, 1064
BtsIMutI CAGTG 3 cut(s) 381, 689, 1001
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 8 cut(s) 60, 147, 198, 765, 803, 807, 966, 1169
CfoI GCGC 8 cut(s) 25, 36, 58, 77, 140, 159, 468, 524
Cfr10I RCCGGY 2 cut(s) 145, 439
Cfr13I GGNCC 1 cut(s) 1141
CseI GACGC 3 cut(s) 148, 921, 1049
Csp6I GTAC 3 cut(s) 69, 668, 839
CspAI ACCGGT 1 cut(s) 439
CspCI CAANNNNNGTGG 2 cut(s) 1256, 1291
CviAII CATG 6 cut(s) 100, 477, 563, 631, 806, 1013
CviQI GTAC 3 cut(s) 69, 668, 839
DdeI CTNAG 5 cut(s) 79, 824, 863, 1050, 1172
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 2 cut(s) 147, 1165
EagI CGGCCG 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 510, 1184
EarI CTCTTC 2 cut(s) 510, 1184
EclXI CGGCCG 1 cut(s) 147
Eco31I GGTCTC 1 cut(s) 1207
Eco52I CGGCCG 1 cut(s) 147
Eco57I CTGAAG 3 cut(s) 193, 764, 1254
Eco72I CACGTG 1 cut(s) 971
Eco91I GGTNACC 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 1141
EcoO65I GGTNACC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 1080
FaeI CATG 6 cut(s) 103, 480, 566, 634, 809, 1016
FaqI GGGAC 1 cut(s) 1014
FatI CATG 6 cut(s) 99, 476, 562, 630, 805, 1012
Fnu4HI GCNGC 7 cut(s) 194, 236, 372, 886, 1092, 1095, 1158
FokI GGATG 5 cut(s) 32, 342, 510, 710, 1051
Fsp4HI GCNGC 7 cut(s) 194, 236, 372, 886, 1092, 1095, 1158
FspBI CTAG 2 cut(s) 1299, 1309
GlaI GCGC 8 cut(s) 24, 35, 57, 76, 139, 158, 467, 523
GluI GCNGC 7 cut(s) 194, 236, 372, 886, 1092, 1095, 1158
GsuI CTGGAG 3 cut(s) 173, 963, 1121
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 3 cut(s) 149, 1143, 1167
HapII CCGG 4 cut(s) 146, 182, 440, 1107
HgaI GACGC 3 cut(s) 148, 921, 1049
HhaI GCGC 8 cut(s) 25, 36, 58, 77, 140, 159, 468, 524
Hin1II CATG 6 cut(s) 103, 480, 566, 634, 809, 1016
Hin6I GCGC 8 cut(s) 23, 34, 56, 75, 138, 157, 466, 522
HinP1I GCGC 8 cut(s) 23, 34, 56, 75, 138, 157, 466, 522
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 5 cut(s) 152, 945, 1103, 1192, 1208
HpaII CCGG 4 cut(s) 146, 182, 440, 1107
HphI GGTGA 3 cut(s) 339, 625, 653
Hpy166II GTNNAC 5 cut(s) 644, 786, 869, 974, 1018
Hpy188I TCNGA 4 cut(s) 178, 1051, 1207, 1213
Hpy188III TCNNGA 5 cut(s) 278, 307, 446, 1100, 1186
Hpy8I GTNNAC 5 cut(s) 644, 786, 869, 974, 1018
HpyAV CCTTC 4 cut(s) 328, 448, 871, 1037
HpyCH4III ACNGT 5 cut(s) 364, 385, 430, 736, 1120
HpyCH4IV ACGT 2 cut(s) 970, 1129
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 146, 163, 519, 709, 938, 1082, 1091, 1157
HpyF3I CTNAG 5 cut(s) 79, 824, 863, 1050, 1172
HpySE526I ACGT 2 cut(s) 970, 1129
Hsp92II CATG 6 cut(s) 103, 480, 566, 634, 809, 1016
HspAI GCGC 8 cut(s) 23, 34, 56, 75, 138, 157, 466, 522
KroI GCCGGC 1 cut(s) 145
KroNI GCCGGC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 47, 814
Lsp1109I GCAGC 7 cut(s) 205, 247, 358, 897, 1078, 1081, 1169
LweI GCATC 7 cut(s) 453, 488, 776, 943, 1138, 1144, 1262
MaeI CTAG 2 cut(s) 1299, 1309
MaeII ACGT 2 cut(s) 970, 1129
MaeIII GTNAC 3 cut(s) 345, 364, 613
MboII GAAGA 3 cut(s) 497, 1201, 1303
MfeI CAATTG 1 cut(s) 834
MhlI GDGCHC 3 cut(s) 44, 871, 966
MlsI TGGCCA 1 cut(s) 1167
MluCI AATT 5 cut(s) 567, 705, 726, 753, 834
MluI ACGCGT 1 cut(s) 913
MluNI TGGCCA 1 cut(s) 1167
MlyI GAGTC 5 cut(s) 146, 954, 1112, 1201, 1217
MmeI TCCRAC 1 cut(s) 384
Mox20I TGGCCA 1 cut(s) 1167
MroNI GCCGGC 1 cut(s) 145
MscI TGGCCA 1 cut(s) 1167
MseI TTAA 5 cut(s) 267, 387, 555, 756, 1032
MslI CAYNNNNRTG 4 cut(s) 98, 635, 682, 969
Msp20I TGGCCA 1 cut(s) 1167
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 4 cut(s) 146, 182, 440, 1107
MspR9I CCNGG 1 cut(s) 1082
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 1082
MvnI CGCG 8 cut(s) 36, 56, 58, 75, 138, 157, 159, 915
MwoI GCNNNNNNNGC 9 cut(s) 31, 146, 163, 519, 709, 938, 1082, 1091, 1157
NaeI GCCGGC 1 cut(s) 147
NgoMIV GCCGGC 1 cut(s) 145
NlaIII CATG 6 cut(s) 103, 480, 566, 634, 809, 1016
NlaIV GGNNCC 3 cut(s) 551, 744, 1142
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 345, 613
NspI RCATGY 2 cut(s) 480, 809
OliI CACNNNNGTG 2 cut(s) 682, 969
PaeI GCATGC 1 cut(s) 809
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 174
PctI GAATGC 1 cut(s) 302
PdiI GCCGGC 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 439
PkrI GCNGC 7 cut(s) 195, 237, 373, 887, 1093, 1096, 1159
PleI GAGTC 5 cut(s) 146, 953, 1111, 1200, 1216
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 5 cut(s) 146, 953, 1111, 1200, 1216
Ppu21I YACGTR 1 cut(s) 971
PshAI GACNNNNGTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 1080
PspCI CACGTG 1 cut(s) 971
PspEI GGTNACC 1 cut(s) 345
PspGI CCWGG 1 cut(s) 1080
PspN4I GGNNCC 3 cut(s) 551, 744, 1142
PspPI GGNCC 1 cut(s) 1141
RsaI GTAC 3 cut(s) 70, 669, 840
RsaNI GTAC 3 cut(s) 69, 668, 839
RseI CAYNNNNRTG 4 cut(s) 98, 635, 682, 969
SaqAI TTAA 5 cut(s) 267, 387, 555, 756, 1032
SatI GCNGC 7 cut(s) 194, 236, 372, 886, 1092, 1095, 1158
Sau96I GGNCC 1 cut(s) 1141
SchI GAGTC 5 cut(s) 146, 954, 1112, 1201, 1217
ScrFI CCNGG 1 cut(s) 1082
SduI GDGCHC 3 cut(s) 44, 871, 966
SfaNI GCATC 7 cut(s) 453, 488, 776, 943, 1138, 1144, 1262
SfcI CTRYAG 2 cut(s) 223, 525
SmiMI CAYNNNNRTG 4 cut(s) 98, 635, 682, 969
SmlI CTYRAG 3 cut(s) 317, 554, 659
SmoI CTYRAG 3 cut(s) 317, 554, 659
SphI GCATGC 1 cut(s) 809
Sse9I AATT 5 cut(s) 567, 705, 726, 753, 834
SspMI CTAG 2 cut(s) 1299, 1309
StyD4I CCNGG 1 cut(s) 1080
TaaI ACNGT 5 cut(s) 364, 385, 430, 736, 1120
TaiI ACGT 2 cut(s) 973, 1132
TaqI TCGA 1 cut(s) 204
TasI AATT 5 cut(s) 567, 705, 726, 753, 834
TatI WGTACW 1 cut(s) 667
Tru1I TTAA 5 cut(s) 267, 387, 555, 756, 1032
Tru9I TTAA 5 cut(s) 267, 387, 555, 756, 1032
TscAI CASTG 3 cut(s) 388, 689, 1001
TseFI GTSAC 2 cut(s) 345, 613
TseI GCWGC 7 cut(s) 193, 235, 371, 885, 1091, 1094, 1157
Tsp45I GTSAC 2 cut(s) 345, 613
TspDTI ATGAA 7 cut(s) 230, 579, 619, 668, 890, 915, 980
TspGWI ACGGA 2 cut(s) 56, 1285
TspRI CASTG 3 cut(s) 388, 689, 1001
Vha464I CTTAAG 1 cut(s) 554
VneI GTGCAC 1 cut(s) 867
XceI RCATGY 2 cut(s) 480, 809
XspI CTAG 2 cut(s) 1299, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.