Rmu_sc0008470.1_g000011

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008470.1
OG0010937
Physical Location & Seq
Reverse (-)
64809 .. 66246
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008470.1_g000011.1.cds

Sequence Viewer

Length: 738 bp
atgatgacagccacatcaacggcgctaatccagcgcgtgtgctccgtccccctcgcgcgtgccgtccgtactcgcgccttggccaccaacaccactaccatgaacctctccaccactcccactccacgcgccaccccggccgactcgagcgtcctgctcggcttgtccgaaccggaactccagcagctcgcccttgacttcagtcagcaaggctacagaggcaagcagcttcatcatctcatttacaaaagaaagattaaagacattcaggatttcagtcaagtgcctctggcattcaggaatgagcttgaggaagctggatggaaggttgggaggtcacctatttacaatactgttactgctgctgatggcactgttaagttattgataaagttggaggataaccgattgattgaaactgttggcataccggttcaagacgagaagggtttgatgcgccttacagcatgtgtctcatcacagttgattttccaggataggattcagtgggatgccctctgcgttgctctttttgcgcaacagaaaaaaggagggttttcaaggaaccttaagaggcatgaaattgttgagcaggttttggccattgaggagatattccagcatagggtgactaatgtagtgttcatgggaatgggtgaaccaatgatgaacctcaagtctgtactcgaagcacaccagtgcttgaataaggtgcattccaacgactttgggcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

245

Amino Acids

27.43

Weight (kDa)

8.91

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc16I TGCGCA 1 cut(s) 537
Acc36I ACCTGC 1 cut(s) 583
AccII CGCG 5 cut(s) 36, 56, 58, 75, 129
AcoI YGGCCR 3 cut(s) 81, 138, 600
AcuI CTGAAG 1 cut(s) 184
AfaI GTAC 2 cut(s) 70, 684
AfiI CCNNNNNNNGG 1 cut(s) 625
AflII CTTAAG 1 cut(s) 569
AgeI ACCGGT 1 cut(s) 430
AgsI TTSAA 4 cut(s) 416, 437, 561, 706
AjnI CCWGG 1 cut(s) 492
AleI CACNNNNGTG 1 cut(s) 697
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 4 cut(s) 187, 229, 307, 317
AluI AGCT 4 cut(s) 187, 229, 307, 317
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 478
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 3 cut(s) 81, 138, 600
ApeKI GCWGC 3 cut(s) 184, 226, 362
AsiGI ACCGGT 1 cut(s) 430
AspLEI GCGC 7 cut(s) 25, 36, 58, 77, 131, 459, 538
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 330, 640, 668
AvaI CYCGRG 1 cut(s) 145
BalI TGGCCA 2 cut(s) 83, 602
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 3 cut(s) 196, 238, 349
BccI CCATC 2 cut(s) 315, 362
BceAI ACGGC 2 cut(s) 36, 47
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 494
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 478
BfmI CTRYAG 1 cut(s) 214
BfoI RGCGCY 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 569
BfuAI ACCTGC 1 cut(s) 583
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 3 cut(s) 185, 227, 363
BlsI GCNGC 3 cut(s) 186, 228, 364
Bme1390I CCNGG 2 cut(s) 137, 494
BmeT110I CYCGRG 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 2 cut(s) 137, 494
BmsI GCATC 2 cut(s) 444, 502
BoxI GACNNNNGTC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 164
BpuEI CTTGAG 2 cut(s) 329, 659
BpuMI CCSGG 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 78, 135
BsaWI WCCGGW 2 cut(s) 172, 430
BsaXI ACNNNNNCTCC 4 cut(s) 106, 136, 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse118I RCCGGY 1 cut(s) 430
Bse1I ACTGG 1 cut(s) 697
BseBI CCWGG 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 78, 135
BseGI GGATG 2 cut(s) 326, 517
BseLI CCNNNNNNNGG 1 cut(s) 625
BseNI ACTGG 1 cut(s) 697
BseRI GAGGAG 1 cut(s) 623
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 3 cut(s) 196, 238, 349
Bsh1236I CGCG 5 cut(s) 36, 56, 58, 75, 129
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 83, 140, 602
BshTI ACCGGT 1 cut(s) 430
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 3 cut(s) 137, 173, 431
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 293, 715
BsnI GGCC 3 cut(s) 83, 140, 602
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 44
BspANI GGCC 3 cut(s) 83, 140, 602
BspFNI CGCG 5 cut(s) 36, 56, 58, 75, 129
BspLI GGNNCC 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 430
BsrI ACTGG 1 cut(s) 697
BssAI RCCGGY 1 cut(s) 430
BssECI CCNNGG 2 cut(s) 78, 135
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 4 cut(s) 355, 376, 421, 483
BstAFI CTTAAG 1 cut(s) 569
BstC8I GCNNGC 3 cut(s) 60, 189, 224
BstEII GGTNACC 1 cut(s) 336
BstF5I GGATG 2 cut(s) 326, 517
BstFNI CGCG 5 cut(s) 36, 56, 58, 75, 129
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 7 cut(s) 25, 36, 58, 77, 131, 459, 538
BstMAI GTCTC 1 cut(s) 478
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 31, 137, 219, 533
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 1 cut(s) 471
BstPAI GACNNNNGTC 1 cut(s) 201
BstPI GGTNACC 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 135, 492
BstSFI CTRYAG 1 cut(s) 214
BstUI CGCG 5 cut(s) 36, 56, 58, 75, 129
BstV1I GCAGC 3 cut(s) 196, 238, 349
BstZI CGGCCG 1 cut(s) 138
BsuRI GGCC 3 cut(s) 83, 140, 602
BtsCI GGATG 2 cut(s) 326, 517
BtsIMutI CAGTG 3 cut(s) 372, 512, 704
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 3 cut(s) 60, 189, 224
CfoI GCGC 7 cut(s) 25, 36, 58, 77, 131, 459, 538
Cfr10I RCCGGY 1 cut(s) 430
CseI GACGC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 69, 683
CspAI ACCGGT 1 cut(s) 430
CviAII CATG 4 cut(s) 100, 468, 578, 646
CviQI GTAC 2 cut(s) 69, 683
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
EaeI YGGCCR 3 cut(s) 81, 138, 600
EagI CGGCCG 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 78
Eco52I CGGCCG 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 145
Eco91I GGTNACC 1 cut(s) 336
EcoO65I GGTNACC 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 492
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 4 cut(s) 103, 471, 581, 649
FaiI YATR 6 cut(s) 101, 428, 469, 579, 624, 647
FaqI GGGAC 1 cut(s) 32
FatI CATG 4 cut(s) 99, 467, 577, 645
Fnu4HI GCNGC 3 cut(s) 185, 227, 363
FokI GGATG 2 cut(s) 333, 524
Fsp4HI GCNGC 3 cut(s) 185, 227, 363
FspI TGCGCA 1 cut(s) 537
GlaI GCGC 7 cut(s) 24, 35, 57, 76, 130, 458, 537
GluI GCNGC 3 cut(s) 185, 227, 363
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 3 cut(s) 83, 140, 602
HapII CCGG 3 cut(s) 137, 173, 431
HgaI GACGC 1 cut(s) 139
HhaI GCGC 7 cut(s) 25, 36, 58, 77, 131, 459, 538
Hin1II CATG 4 cut(s) 103, 471, 581, 649
Hin6I GCGC 7 cut(s) 23, 34, 56, 75, 129, 457, 536
HinP1I GCGC 7 cut(s) 23, 34, 56, 75, 129, 457, 536
HinfI GANTC 2 cut(s) 143, 502
HpaII CCGG 3 cut(s) 137, 173, 431
HphI GGTGA 3 cut(s) 330, 640, 668
Hpy166II GTNNAC 1 cut(s) 659
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 3 cut(s) 269, 298, 437
Hpy8I GTNNAC 1 cut(s) 659
HpyAV CCTTC 2 cut(s) 319, 439
HpyCH4III ACNGT 4 cut(s) 355, 376, 421, 483
HpyCH4V TGCA 1 cut(s) 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 137, 219, 533
Hsp92II CATG 4 cut(s) 103, 471, 581, 649
HspAI GCGC 7 cut(s) 23, 34, 56, 75, 129, 457, 536
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 3 cut(s) 196, 238, 349
LweI GCATC 2 cut(s) 444, 502
MaeIII GTNAC 3 cut(s) 336, 355, 628
MhlI GDGCHC 1 cut(s) 44
MlsI TGGCCA 2 cut(s) 83, 602
MluCI AATT 1 cut(s) 582
MluNI TGGCCA 2 cut(s) 83, 602
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 375
Mox20I TGGCCA 2 cut(s) 83, 602
MscI TGGCCA 2 cut(s) 83, 602
MseI TTAA 3 cut(s) 258, 378, 570
MslI CAYNNNNRTG 3 cut(s) 98, 650, 697
Msp20I TGGCCA 2 cut(s) 83, 602
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 3 cut(s) 137, 173, 431
MspR9I CCNGG 2 cut(s) 137, 494
Mva1269I GAATGC 2 cut(s) 293, 715
MvaI CCWGG 1 cut(s) 494
MvnI CGCG 5 cut(s) 36, 56, 58, 75, 129
MwoI GCNNNNNNNGC 4 cut(s) 31, 137, 219, 533
NciI CCSGG 1 cut(s) 137
NlaIII CATG 4 cut(s) 103, 471, 581, 649
NlaIV GGNNCC 1 cut(s) 566
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 336, 628
NsbI TGCGCA 1 cut(s) 537
NspI RCATGY 1 cut(s) 471
OliI CACNNNNGTG 1 cut(s) 697
PaeR7I CTCGAG 1 cut(s) 145
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 165
PctI GAATGC 2 cut(s) 293, 715
PfeI GAWTC 1 cut(s) 502
PfoI TCCNGGA 1 cut(s) 492
PinAI ACCGGT 1 cut(s) 430
PkrI GCNGC 3 cut(s) 186, 228, 364
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PshAI GACNNNNGTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 492
PspEI GGTNACC 1 cut(s) 336
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 566
PspXI VCTCGAGB 1 cut(s) 145
RsaI GTAC 2 cut(s) 70, 684
RsaNI GTAC 2 cut(s) 69, 683
RseI CAYNNNNRTG 3 cut(s) 98, 650, 697
SaqAI TTAA 3 cut(s) 258, 378, 570
SatI GCNGC 3 cut(s) 185, 227, 363
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 137, 494
SduI GDGCHC 1 cut(s) 44
SfaNI GCATC 2 cut(s) 444, 502
SfcI CTRYAG 1 cut(s) 214
Sfr274I CTCGAG 1 cut(s) 145
SlaI CTCGAG 1 cut(s) 145
SmiMI CAYNNNNRTG 3 cut(s) 98, 650, 697
SmlI CTYRAG 4 cut(s) 145, 308, 569, 674
SmoI CTYRAG 4 cut(s) 145, 308, 569, 674
Sse9I AATT 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 135, 492
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 4 cut(s) 355, 376, 421, 483
TaqI TCGA 2 cut(s) 146, 687
TasI AATT 1 cut(s) 582
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 3 cut(s) 258, 378, 570
Tru9I TTAA 3 cut(s) 258, 378, 570
TscAI CASTG 3 cut(s) 379, 512, 704
TseFI GTSAC 2 cut(s) 336, 628
TseI GCWGC 3 cut(s) 184, 226, 362
Tsp45I GTSAC 2 cut(s) 336, 628
TspDTI ATGAA 5 cut(s) 116, 221, 594, 634, 683
TspGWI ACGGA 2 cut(s) 34, 56
TspRI CASTG 3 cut(s) 379, 512, 704
Vha464I CTTAAG 1 cut(s) 569
XceI RCATGY 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.