Rroxscaffold_4G00289290

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010937
Physical Location & Seq
Reverse (-)
9940874 .. 9944974
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00289290.1

Sequence Viewer

Length: 1296 bp
ATGACAGCCACATCAACGGCGCTAATTCAGCGCGTGTGCTCCGTCCCCCTCGCGCGTGCCGTCCGTACTCGCGCCTTGGCCACCAACACCACCACCACCACCATGAACCTCTCCACCACTCCCACTCCACGCGCCTCCCCGGCCGACTCGCGCATCCTGCTCGGCTTGTCCGAACCGGAACTCCAGCAGCTCGCCCTTGACTTCAGTCAGCAAGGCTACAGAGGCAAGCAGCTTCATCATCTCATTTACAAAAGAAAGATTAAAGACATTCAGGATTTCAGTCAAGTGCCTCTGGCATTCAGGAATGAGCTTGAGGAAGCTGGATGGAAGGTTGGGAGGTCACCTATTTACAATACTGTTACTGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACCGATTGATTGAAACTGTTGGCATACCGGTTCAAGACGAGAAAGGTTTGATGCGCCTTACAGCTTGTGTCTCATCACAGGTGGGATGCCCTCTGCGTTGCTCTTTTTGCGCAACAGGAAAAGGAGGGTTTTCAAGGAACCTTAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAGATATTCCAGCATAGGGTGACTAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTCAAGTCTGTACTTGAAGCACACCAGTGCTTGAATAAGGATGTGCAAATTGGGCAAAGAATGATGACAATTTCTACTGTGGGGGTTCCAAATACAATTAAAAGGCTGGCATCTCACAAGCTTCAGTCAACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAACTTAGGGAAACAATTGTACCAAGTGCGAAAGCATATCCTATAAGTGCACTTATGAAGGATTGCAGCGACTATTTCAATGAAACAAATAGACGCGTTTCTTTTGAGTATGCCCTTTTAGCTGGAGTCAATGATGCAGTAGAGCATGCACGTGAACTTGCAGAGCTACTTCATCAGTGGGGACGAGGTTATCATGTGAACTTGATACCTTTTAATCCAATAGAAGGTTCTGAGTATAAGCGTCCATCCAAGAGAGCTGTCCAGGCATTTGCTGCTGCTCTGGAGTCTGGTAAAATAACTGTTAGCACACGTCAAACAAGGGGCCTTGATGCAGATGCAGCTTGTGGCCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGGTCTCCGAGTCTGACAACTTACAACCTGAACCTGAAGCAGATGTTGCAGTTGCATCAGTAAGAAGCTCAACGGAAGTGGTGTTCAAAGTTGAAGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

431

Amino Acids

47.61

Weight (kDa)

8.92

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 160 - 323 1.8e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 517
Acc36I ACCTGC 1 cut(s) 562
AccII CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
AcoI YGGCCR 3 cut(s) 78, 141, 1159
AcuI CTGAAG 3 cut(s) 187, 758, 1248
AcvI CACGTG 1 cut(s) 965
AfaI GTAC 3 cut(s) 67, 663, 834
AfiI CCNNNNNNNGG 2 cut(s) 604, 1037
AflII CTTAAG 1 cut(s) 548
AflIII ACRYGT 2 cut(s) 907, 1121
AgeI ACCGGT 1 cut(s) 433
AgsI TTSAA 8 cut(s) 419, 440, 540, 668, 685, 892, 1279, 1286
AjiI CACGTC 1 cut(s) 1124
AjnI CCWGG 1 cut(s) 1074
AjuI GAANNNNNNNTTGG 4 cut(s) 1024, 1056, 1175, 1207
AleI CACNNNNGTG 1 cut(s) 676
AloI GAACNNNNNNTCC 4 cut(s) 165, 197, 1259, 1291
Alw21I GWGCWC 2 cut(s) 41, 865
Alw26I GTCTC 2 cut(s) 481, 1201
Alw44I GTGCAC 1 cut(s) 861
AoxI GGCC 4 cut(s) 78, 141, 1135, 1159
ApaLI GTGCAC 1 cut(s) 861
ApeKI GCWGC 7 cut(s) 187, 229, 365, 879, 1085, 1088, 1151
AsiGI ACCGGT 1 cut(s) 433
AspLEI GCGC 8 cut(s) 22, 33, 55, 74, 134, 153, 462, 518
AspS9I GGNCC 1 cut(s) 1135
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 3 cut(s) 333, 619, 647
BaeGI GKGCMC 1 cut(s) 865
BaeI ACNNNNGTAYC 2 cut(s) 816, 849
BalI TGGCCA 2 cut(s) 80, 1161
BbrPI CACGTG 1 cut(s) 965
Bbv12I GWGCWC 2 cut(s) 41, 865
BbvI GCAGC 7 cut(s) 199, 241, 352, 891, 1072, 1075, 1163
BccI CCATC 3 cut(s) 318, 365, 1066
BceAI ACGGC 2 cut(s) 33, 44
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 1 cut(s) 1076
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 481, 1201
BfaI CTAG 1 cut(s) 1294
BfmI CTRYAG 1 cut(s) 217
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 562
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 7 cut(s) 188, 230, 366, 880, 1086, 1089, 1152
BlsI GCNGC 7 cut(s) 189, 231, 367, 881, 1087, 1090, 1153
Bme1390I CCNGG 2 cut(s) 140, 1076
BmgBI CACGTC 1 cut(s) 1124
BmgT120I GGNCC 1 cut(s) 1135
BmiI GGNNCC 3 cut(s) 545, 738, 1136
BmrFI CCNGG 2 cut(s) 140, 1076
BmsI GCATC 8 cut(s) 162, 447, 482, 770, 937, 1132, 1138, 1256
BoxI GACNNNNGTC 1 cut(s) 204
BpmI CTGGAG 3 cut(s) 167, 957, 1115
BpuEI CTTGAG 2 cut(s) 332, 638
BpuMI CCSGG 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 965
BsaI GGTCTC 1 cut(s) 1201
BsaJI CCNNGG 2 cut(s) 75, 138
BsaWI WCCGGW 2 cut(s) 175, 433
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 165, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 1037
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 1 cut(s) 676
BseBI CCWGG 1 cut(s) 1076
BseDI CCNNGG 2 cut(s) 75, 138
BseGI GGATG 5 cut(s) 153, 329, 497, 697, 1058
BseLI CCNNNNNNNGG 2 cut(s) 604, 1037
BseMII CTCAG 1 cut(s) 1035
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 865
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 7 cut(s) 199, 241, 352, 891, 1072, 1075, 1163
Bsh1236I CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 4 cut(s) 80, 143, 1137, 1161
BshTI ACCGGT 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 41, 865
BsiSI CCGG 3 cut(s) 140, 176, 434
BslFI GGGAC 2 cut(s) 29, 1008
BslI CCNNNNNNNGG 2 cut(s) 604, 1037
BsmAI GTCTC 2 cut(s) 481, 1201
BsmFI GGGAC 2 cut(s) 29, 1008
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 4 cut(s) 80, 143, 1137, 1161
Bso31I GGTCTC 1 cut(s) 1201
Bsp1286I GDGCHC 2 cut(s) 41, 865
BspANI GGCC 4 cut(s) 80, 143, 1137, 1161
BspCNI CTCAG 1 cut(s) 1036
BspFNI CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
BspLI GGNNCC 3 cut(s) 545, 738, 1136
BspMI ACCTGC 1 cut(s) 562
BspTI CTTAAG 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 1201
BsrFI RCCGGY 1 cut(s) 433
BsrI ACTGG 1 cut(s) 676
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 75, 138
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 1076
Bst4CI ACNGT 5 cut(s) 358, 379, 424, 730, 1114
Bst6I CTCTTC 1 cut(s) 1178
BstAFI CTTAAG 1 cut(s) 548
BstAPI GCANNNNNTGC 2 cut(s) 1085, 1238
BstBAI YACGTR 1 cut(s) 965
BstC8I GCNNGC 8 cut(s) 57, 192, 227, 759, 797, 801, 960, 1163
BstDEI CTNAG 3 cut(s) 818, 1044, 1166
BstEII GGTNACC 1 cut(s) 339
BstF5I GGATG 5 cut(s) 153, 329, 497, 697, 1058
BstFNI CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 8 cut(s) 22, 33, 55, 74, 134, 153, 462, 518
BstMAI GTCTC 2 cut(s) 481, 1201
BstMCI CGRYCG 1 cut(s) 144
BstNI CCWGG 1 cut(s) 1076
BstNSI RCATGY 2 cut(s) 803, 962
BstPAI GACNNNNGTC 1 cut(s) 204
BstPI GGTNACC 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 138, 1074
BstSFI CTRYAG 1 cut(s) 217
BstSLI GKGCMC 1 cut(s) 865
BstUI CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
BstV1I GCAGC 7 cut(s) 199, 241, 352, 891, 1072, 1075, 1163
BstZI CGGCCG 1 cut(s) 141
BsuRI GGCC 4 cut(s) 80, 143, 1137, 1161
BtrI CACGTC 1 cut(s) 1124
BtsCI GGATG 5 cut(s) 153, 329, 497, 697, 1058
BtsIMutI CAGTG 3 cut(s) 375, 683, 995
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 8 cut(s) 57, 192, 227, 759, 797, 801, 960, 1163
CfoI GCGC 8 cut(s) 22, 33, 55, 74, 134, 153, 462, 518
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 1135
CseI GACGC 2 cut(s) 915, 1043
Csp6I GTAC 3 cut(s) 66, 662, 833
CspAI ACCGGT 1 cut(s) 433
CspCI CAANNNNNGTGG 2 cut(s) 1251, 1286
CviAII CATG 6 cut(s) 103, 557, 625, 800, 959, 1007
CviQI GTAC 3 cut(s) 66, 662, 833
DdeI CTNAG 3 cut(s) 818, 1044, 1166
EaeI YGGCCR 3 cut(s) 78, 141, 1159
EagI CGGCCG 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 1178
EarI CTCTTC 1 cut(s) 1178
EclXI CGGCCG 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 1201
Eco52I CGGCCG 1 cut(s) 141
Eco57I CTGAAG 3 cut(s) 187, 758, 1248
Eco72I CACGTG 1 cut(s) 965
Eco91I GGTNACC 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 1135
EcoO65I GGTNACC 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 1074
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 6 cut(s) 106, 560, 628, 803, 962, 1010
FaqI GGGAC 2 cut(s) 29, 1008
FatI CATG 6 cut(s) 102, 556, 624, 799, 958, 1006
Fnu4HI GCNGC 7 cut(s) 188, 230, 366, 880, 1086, 1089, 1152
FokI GGATG 5 cut(s) 140, 336, 504, 704, 1045
Fsp4HI GCNGC 7 cut(s) 188, 230, 366, 880, 1086, 1089, 1152
FspBI CTAG 1 cut(s) 1294
FspI TGCGCA 1 cut(s) 517
GlaI GCGC 8 cut(s) 21, 32, 54, 73, 133, 152, 461, 517
GluI GCNGC 7 cut(s) 188, 230, 366, 880, 1086, 1089, 1152
GsuI CTGGAG 3 cut(s) 167, 957, 1115
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 80, 143, 1137, 1161
HapII CCGG 3 cut(s) 140, 176, 434
HgaI GACGC 2 cut(s) 915, 1043
HhaI GCGC 8 cut(s) 22, 33, 55, 74, 134, 153, 462, 518
Hin1II CATG 6 cut(s) 106, 560, 628, 803, 962, 1010
Hin6I GCGC 8 cut(s) 20, 31, 53, 72, 132, 151, 460, 516
HinP1I GCGC 8 cut(s) 20, 31, 53, 72, 132, 151, 460, 516
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 770
HinfI GANTC 5 cut(s) 146, 939, 1097, 1186, 1202
HpaII CCGG 3 cut(s) 140, 176, 434
HphI GGTGA 3 cut(s) 333, 619, 647
Hpy166II GTNNAC 5 cut(s) 638, 780, 863, 968, 1012
Hpy188I TCNGA 4 cut(s) 172, 1045, 1201, 1207
Hpy188III TCNNGA 5 cut(s) 272, 301, 440, 1094, 1180
Hpy8I GTNNAC 5 cut(s) 638, 780, 863, 968, 1012
HpyAV CCTTC 3 cut(s) 322, 865, 1031
HpyCH4III ACNGT 5 cut(s) 358, 379, 424, 730, 1114
HpyCH4IV ACGT 2 cut(s) 964, 1123
HpyF3I CTNAG 3 cut(s) 818, 1044, 1166
HpySE526I ACGT 2 cut(s) 964, 1123
Hsp92II CATG 6 cut(s) 106, 560, 628, 803, 962, 1010
HspAI GCGC 8 cut(s) 20, 31, 53, 72, 132, 151, 460, 516
LmnI GCTCC 2 cut(s) 44, 808
Lsp1109I GCAGC 7 cut(s) 199, 241, 352, 891, 1072, 1075, 1163
LweI GCATC 8 cut(s) 162, 447, 482, 770, 937, 1132, 1138, 1256
MaeI CTAG 1 cut(s) 1294
MaeII ACGT 2 cut(s) 964, 1123
MaeIII GTNAC 3 cut(s) 339, 358, 607
MboII GAAGA 1 cut(s) 1195
MfeI CAATTG 1 cut(s) 828
MhlI GDGCHC 2 cut(s) 41, 865
MlsI TGGCCA 2 cut(s) 80, 1161
MluCI AATT 6 cut(s) 24, 561, 699, 720, 747, 828
MluI ACGCGT 1 cut(s) 907
MluNI TGGCCA 2 cut(s) 80, 1161
MlyI GAGTC 5 cut(s) 140, 948, 1106, 1195, 1211
MmeI TCCRAC 1 cut(s) 378
Mox20I TGGCCA 2 cut(s) 80, 1161
MscI TGGCCA 2 cut(s) 80, 1161
MseI TTAA 5 cut(s) 261, 381, 549, 750, 1026
MslI CAYNNNNRTG 4 cut(s) 101, 629, 676, 963
Msp20I TGGCCA 2 cut(s) 80, 1161
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 3 cut(s) 140, 176, 434
MspR9I CCNGG 2 cut(s) 140, 1076
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 1076
MvnI CGCG 7 cut(s) 33, 53, 55, 72, 132, 151, 909
NciI CCSGG 1 cut(s) 140
NlaIII CATG 6 cut(s) 106, 560, 628, 803, 962, 1010
NlaIV GGNNCC 3 cut(s) 545, 738, 1136
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 339, 607
NsbI TGCGCA 1 cut(s) 517
NspI RCATGY 2 cut(s) 803, 962
OliI CACNNNNGTG 1 cut(s) 676
PaeI GCATGC 2 cut(s) 803, 962
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 168
PctI GAATGC 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 433
PkrI GCNGC 7 cut(s) 189, 231, 367, 881, 1087, 1090, 1153
PleI GAGTC 5 cut(s) 140, 947, 1105, 1194, 1210
PmaCI CACGTG 1 cut(s) 965
PmlI CACGTG 1 cut(s) 965
PpsI GAGTC 5 cut(s) 140, 947, 1105, 1194, 1210
Ppu21I YACGTR 1 cut(s) 965
PshAI GACNNNNGTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 1074
PspCI CACGTG 1 cut(s) 965
PspEI GGTNACC 1 cut(s) 339
PspGI CCWGG 1 cut(s) 1074
PspN4I GGNNCC 3 cut(s) 545, 738, 1136
PspPI GGNCC 1 cut(s) 1135
RsaI GTAC 3 cut(s) 67, 663, 834
RsaNI GTAC 3 cut(s) 66, 662, 833
RseI CAYNNNNRTG 4 cut(s) 101, 629, 676, 963
SaqAI TTAA 5 cut(s) 261, 381, 549, 750, 1026
SatI GCNGC 7 cut(s) 188, 230, 366, 880, 1086, 1089, 1152
Sau96I GGNCC 1 cut(s) 1135
SchI GAGTC 5 cut(s) 140, 948, 1106, 1195, 1211
ScrFI CCNGG 2 cut(s) 140, 1076
SduI GDGCHC 2 cut(s) 41, 865
SfaNI GCATC 8 cut(s) 162, 447, 482, 770, 937, 1132, 1138, 1256
SfcI CTRYAG 1 cut(s) 217
SmiMI CAYNNNNRTG 4 cut(s) 101, 629, 676, 963
SmlI CTYRAG 3 cut(s) 311, 548, 653
SmoI CTYRAG 3 cut(s) 311, 548, 653
SphI GCATGC 2 cut(s) 803, 962
Sse9I AATT 6 cut(s) 24, 561, 699, 720, 747, 828
SspMI CTAG 1 cut(s) 1294
StyD4I CCNGG 2 cut(s) 138, 1074
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 5 cut(s) 358, 379, 424, 730, 1114
TaiI ACGT 2 cut(s) 967, 1126
TasI AATT 6 cut(s) 24, 561, 699, 720, 747, 828
TatI WGTACW 1 cut(s) 661
Tru1I TTAA 5 cut(s) 261, 381, 549, 750, 1026
Tru9I TTAA 5 cut(s) 261, 381, 549, 750, 1026
TscAI CASTG 3 cut(s) 382, 683, 995
TseFI GTSAC 2 cut(s) 339, 607
TseI GCWGC 7 cut(s) 187, 229, 365, 879, 1085, 1088, 1151
Tsp45I GTSAC 2 cut(s) 339, 607
TspDTI ATGAA 8 cut(s) 119, 224, 573, 613, 662, 884, 909, 974
TspGWI ACGGA 3 cut(s) 31, 53, 1280
TspRI CASTG 3 cut(s) 382, 683, 995
Vha464I CTTAAG 1 cut(s) 548
VneI GTGCAC 1 cut(s) 861
XceI RCATGY 2 cut(s) 803, 962
XspI CTAG 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.