Rh1AG343200

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0010937
Physical Location & Seq
Forward (+)
57926724 .. 57929971
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG343200.1

Sequence Viewer

Length: 1248 bp
ATGATGACAGCCACATCAACGGCGCTAATCCAGCGCGTGTGCTCCGTCCCCCTCGCGTGTGCCGTCCGTACTCGCGCCTTGGCCACCACCACCACCATGAACCTCTCCACCACTCCTACTCCACGCGCCTCCCCGGCCGACTCGCGCGTCCTGCTCGGCTTGTCCGAACCGGAACTCCAGCAGCTCGCCCTTGACTTCAGTCAGCAAGGCTACAGAGGCAAGCAGCTTCATCATCTCATTTACAAAAGAAAGATTAAAGACATTCAGGATTTCAGTCAAGTGCCTCTGGCATTCAGGAATGAGCTTGAGGAAGCTGGATGGAAGGTTGGGAGGTCACCTATTTACAATACTGTTACTGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACCGATTGATTGAAACTGTTGGCATACCGGTTCAAGACGAGAAGGGTTTGATGCGCCTTACAGCATGTGTCTCATCACAGGTGGGATGCCCTCTGCGTTGCTCTTTTTGCGCAACAGGAAAAGGAGGGTTTTCAAGGAACCTTAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAGATATTCCAGCATAGGGTGACTAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTCAAGTCTGTACTTGAAGCACACCATTGCTTGAATAAGGATGTGCAAATTGGGCAAAGAATGATGACAATTTCTACTGTGGGGGTTCCAAATACAATTAAAAGGCTGGCATCTCACAAGCTTCAGTCAACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAACTTAGGGAAACAATTGTACCAAGTGCGAAAGCATATCCTCTAAGTGCACTTATGAAGGATTGCAGCGACTATTTCAATGAAACAAATAGGCGCGTTTCCTTTGAGTATGCGCTTTTAGCTGGAGTCAATGATGCAGTAGAGCACGCACGTGAACTTGCAGAGCTACTTCATCAGTGGGGACGAGGTTATCATGTGAACTTGATACCTTTTAATCCAATAGAAGGTTCTGAGTATAAGCGTCCATCCAAGAGAGCTGTCCAGGCATTTGCTGCTGCTCTGGAGTCCGGTAAAATAACTGTTAGCACACGTCAAACAAGGGGCCTTGATGCAGATGCAGCTTGTGGCCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGGTCTCCGAGTCTGACAACTTGCAACCTGAACCTGAAGCAGATGTTGCAGTTGCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

415

Amino Acids

45.79

Weight (kDa)

8.93

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 158 - 321 3.4e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
Acc16I TGCGCA 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 556
AccII CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
AcoI YGGCCR 3 cut(s) 81, 135, 1153
AcuI CTGAAG 3 cut(s) 181, 752, 1242
AcvI CACGTG 1 cut(s) 959
AfaI GTAC 3 cut(s) 70, 657, 828
AfiI CCNNNNNNNGG 2 cut(s) 598, 1031
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 1115
AgeI ACCGGT 1 cut(s) 427
AgsI TTSAA 6 cut(s) 413, 434, 534, 662, 679, 886
AjiI CACGTC 1 cut(s) 1118
AjnI CCWGG 1 cut(s) 1068
AjuI GAANNNNNNNTTGG 4 cut(s) 1018, 1050, 1169, 1201
AleI CACNNNNGTG 1 cut(s) 957
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw21I GWGCWC 3 cut(s) 44, 859, 954
Alw26I GTCTC 2 cut(s) 475, 1195
Alw44I GTGCAC 1 cut(s) 855
AoxI GGCC 4 cut(s) 81, 135, 1129, 1153
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 7 cut(s) 181, 223, 359, 873, 1079, 1082, 1145
AsiGI ACCGGT 1 cut(s) 427
AspLEI GCGC 9 cut(s) 25, 36, 77, 128, 147, 456, 512, 903, 922
AspS9I GGNCC 1 cut(s) 1129
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 3 cut(s) 327, 613, 641
BaeGI GKGCMC 1 cut(s) 859
BaeI ACNNNNGTAYC 2 cut(s) 810, 843
BalI TGGCCA 2 cut(s) 83, 1155
BbrPI CACGTG 1 cut(s) 959
Bbv12I GWGCWC 3 cut(s) 44, 859, 954
BbvI GCAGC 7 cut(s) 193, 235, 346, 885, 1066, 1069, 1157
BccI CCATC 3 cut(s) 312, 359, 1060
BceAI ACGGC 2 cut(s) 36, 47
BciT130I CCWGG 1 cut(s) 1070
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 2 cut(s) 475, 1195
BfmI CTRYAG 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 556
BglI GCCNNNNNGGC 1 cut(s) 134
BisI GCNGC 7 cut(s) 182, 224, 360, 874, 1080, 1083, 1146
BlsI GCNGC 7 cut(s) 183, 225, 361, 875, 1081, 1084, 1147
Bme1390I CCNGG 2 cut(s) 134, 1070
BmgBI CACGTC 1 cut(s) 1118
BmgT120I GGNCC 1 cut(s) 1129
BmiI GGNNCC 3 cut(s) 539, 732, 1130
BmrFI CCNGG 2 cut(s) 134, 1070
BmsI GCATC 6 cut(s) 441, 476, 764, 931, 1126, 1132
BoxI GACNNNNGTC 1 cut(s) 198
BpmI CTGGAG 3 cut(s) 161, 951, 1109
BpuEI CTTGAG 2 cut(s) 326, 632
BpuMI CCSGG 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 959
BsaI GGTCTC 1 cut(s) 1195
BsaJI CCNNGG 2 cut(s) 78, 132
BsaWI WCCGGW 3 cut(s) 169, 427, 1094
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 159, 189
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 1031
Bse118I RCCGGY 1 cut(s) 427
Bse3DI GCAATG 1 cut(s) 670
BseBI CCWGG 1 cut(s) 1070
BseDI CCNNGG 2 cut(s) 78, 132
BseGI GGATG 4 cut(s) 323, 491, 691, 1052
BseLI CCNNNNNNNGG 2 cut(s) 598, 1031
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 1 cut(s) 1029
BseRI GAGGAG 1 cut(s) 596
BseSI GKGCMC 1 cut(s) 859
BseX3I CGGCCG 1 cut(s) 135
BseXI GCAGC 7 cut(s) 193, 235, 346, 885, 1066, 1069, 1157
Bsh1236I CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 4 cut(s) 83, 137, 1131, 1155
BshTI ACCGGT 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 3 cut(s) 44, 859, 954
BsiSI CCGG 4 cut(s) 134, 170, 428, 1095
BslFI GGGAC 2 cut(s) 32, 1002
BslI CCNNNNNNNGG 2 cut(s) 598, 1031
BsmAI GTCTC 2 cut(s) 475, 1195
BsmFI GGGAC 2 cut(s) 32, 1002
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 4 cut(s) 83, 137, 1131, 1155
Bso31I GGTCTC 1 cut(s) 1195
Bsp1286I GDGCHC 3 cut(s) 44, 859, 954
BspANI GGCC 4 cut(s) 83, 137, 1131, 1155
BspCNI CTCAG 1 cut(s) 1030
BspFNI CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
BspLI GGNNCC 3 cut(s) 539, 732, 1130
BspMI ACCTGC 1 cut(s) 556
BspTI CTTAAG 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 1195
BsrDI GCAATG 1 cut(s) 670
BsrFI RCCGGY 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 78, 132
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 1070
Bst4CI ACNGT 5 cut(s) 352, 373, 418, 724, 1108
Bst6I CTCTTC 1 cut(s) 1172
BstAFI CTTAAG 1 cut(s) 542
BstAPI GCANNNNNTGC 2 cut(s) 1079, 1232
BstBAI YACGTR 1 cut(s) 959
BstC8I GCNNGC 7 cut(s) 186, 221, 753, 791, 795, 954, 1157
BstDEI CTNAG 4 cut(s) 812, 851, 1038, 1160
BstEII GGTNACC 1 cut(s) 333
BstF5I GGATG 4 cut(s) 323, 491, 691, 1052
BstFNI CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 9 cut(s) 25, 36, 77, 128, 147, 456, 512, 903, 922
BstMAI GTCTC 2 cut(s) 475, 1195
BstMCI CGRYCG 1 cut(s) 138
BstNI CCWGG 1 cut(s) 1070
BstNSI RCATGY 3 cut(s) 468, 797, 1245
BstPAI GACNNNNGTC 1 cut(s) 198
BstPI GGTNACC 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 132, 1068
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
BstV1I GCAGC 7 cut(s) 193, 235, 346, 885, 1066, 1069, 1157
BstZI CGGCCG 1 cut(s) 135
BsuRI GGCC 4 cut(s) 83, 137, 1131, 1155
BtrI CACGTC 1 cut(s) 1118
BtsCI GGATG 4 cut(s) 323, 491, 691, 1052
BtsIMutI CAGTG 2 cut(s) 369, 989
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 7 cut(s) 186, 221, 753, 791, 795, 954, 1157
CfoI GCGC 9 cut(s) 25, 36, 77, 128, 147, 456, 512, 903, 922
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 1 cut(s) 1129
CseI GACGC 2 cut(s) 136, 1037
Csp6I GTAC 3 cut(s) 69, 656, 827
CspAI ACCGGT 1 cut(s) 427
CviAII CATG 7 cut(s) 97, 465, 551, 619, 794, 1001, 1242
CviQI GTAC 3 cut(s) 69, 656, 827
DdeI CTNAG 4 cut(s) 812, 851, 1038, 1160
DrdI GACNNNNNNGTC 1 cut(s) 146
DseDI GACNNNNNNGTC 1 cut(s) 146
EaeI YGGCCR 3 cut(s) 81, 135, 1153
EagI CGGCCG 1 cut(s) 135
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
EclXI CGGCCG 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 1195
Eco52I CGGCCG 1 cut(s) 135
Eco57I CTGAAG 3 cut(s) 181, 752, 1242
Eco72I CACGTG 1 cut(s) 959
Eco91I GGTNACC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 1129
EcoO65I GGTNACC 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 1068
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 7 cut(s) 100, 468, 554, 622, 797, 1004, 1245
FaqI GGGAC 2 cut(s) 32, 1002
FatI CATG 7 cut(s) 96, 464, 550, 618, 793, 1000, 1241
Fnu4HI GCNGC 7 cut(s) 182, 224, 360, 874, 1080, 1083, 1146
FokI GGATG 4 cut(s) 330, 498, 698, 1039
Fsp4HI GCNGC 7 cut(s) 182, 224, 360, 874, 1080, 1083, 1146
FspI TGCGCA 1 cut(s) 511
GlaI GCGC 9 cut(s) 24, 35, 76, 127, 146, 455, 511, 902, 921
GluI GCNGC 7 cut(s) 182, 224, 360, 874, 1080, 1083, 1146
GsuI CTGGAG 3 cut(s) 161, 951, 1109
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 4 cut(s) 83, 137, 1131, 1155
HapII CCGG 4 cut(s) 134, 170, 428, 1095
HgaI GACGC 2 cut(s) 136, 1037
HhaI GCGC 9 cut(s) 25, 36, 77, 128, 147, 456, 512, 903, 922
Hin1II CATG 7 cut(s) 100, 468, 554, 622, 797, 1004, 1245
Hin6I GCGC 9 cut(s) 23, 34, 75, 126, 145, 454, 510, 901, 920
HinP1I GCGC 9 cut(s) 23, 34, 75, 126, 145, 454, 510, 901, 920
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 5 cut(s) 140, 933, 1091, 1180, 1196
HpaII CCGG 4 cut(s) 134, 170, 428, 1095
HphI GGTGA 3 cut(s) 327, 613, 641
Hpy166II GTNNAC 5 cut(s) 632, 774, 857, 962, 1006
Hpy188I TCNGA 4 cut(s) 166, 1039, 1195, 1201
Hpy188III TCNNGA 5 cut(s) 266, 295, 434, 1088, 1174
Hpy8I GTNNAC 5 cut(s) 632, 774, 857, 962, 1006
HpyAV CCTTC 4 cut(s) 316, 436, 859, 1025
HpyCH4III ACNGT 5 cut(s) 352, 373, 418, 724, 1108
HpyCH4IV ACGT 2 cut(s) 958, 1117
HpyF3I CTNAG 4 cut(s) 812, 851, 1038, 1160
HpySE526I ACGT 2 cut(s) 958, 1117
Hsp92II CATG 7 cut(s) 100, 468, 554, 622, 797, 1004, 1245
HspAI GCGC 9 cut(s) 23, 34, 75, 126, 145, 454, 510, 901, 920
LmnI GCTCC 2 cut(s) 47, 802
Lsp1109I GCAGC 7 cut(s) 193, 235, 346, 885, 1066, 1069, 1157
LweI GCATC 6 cut(s) 441, 476, 764, 931, 1126, 1132
MaeII ACGT 2 cut(s) 958, 1117
MaeIII GTNAC 3 cut(s) 333, 352, 601
MboII GAAGA 1 cut(s) 1189
MfeI CAATTG 1 cut(s) 822
MhlI GDGCHC 3 cut(s) 44, 859, 954
MlsI TGGCCA 2 cut(s) 83, 1155
MluCI AATT 5 cut(s) 555, 693, 714, 741, 822
MluNI TGGCCA 2 cut(s) 83, 1155
MlyI GAGTC 5 cut(s) 134, 942, 1100, 1189, 1205
MmeI TCCRAC 1 cut(s) 372
Mox20I TGGCCA 2 cut(s) 83, 1155
MscI TGGCCA 2 cut(s) 83, 1155
MseI TTAA 5 cut(s) 255, 375, 543, 744, 1020
MslI CAYNNNNRTG 3 cut(s) 95, 623, 957
Msp20I TGGCCA 2 cut(s) 83, 1155
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 4 cut(s) 134, 170, 428, 1095
MspR9I CCNGG 2 cut(s) 134, 1070
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 1 cut(s) 1070
MvnI CGCG 7 cut(s) 36, 56, 75, 126, 145, 147, 903
NciI CCSGG 1 cut(s) 134
NlaIII CATG 7 cut(s) 100, 468, 554, 622, 797, 1004, 1245
NlaIV GGNNCC 3 cut(s) 539, 732, 1130
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 2 cut(s) 333, 601
NsbI TGCGCA 1 cut(s) 511
NspI RCATGY 3 cut(s) 468, 797, 1245
OliI CACNNNNGTG 1 cut(s) 957
PaeI GCATGC 1 cut(s) 797
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 162
PctI GAATGC 1 cut(s) 290
PinAI ACCGGT 1 cut(s) 427
PkrI GCNGC 7 cut(s) 183, 225, 361, 875, 1081, 1084, 1147
PleI GAGTC 5 cut(s) 134, 941, 1099, 1188, 1204
PmaCI CACGTG 1 cut(s) 959
PmlI CACGTG 1 cut(s) 959
PpsI GAGTC 5 cut(s) 134, 941, 1099, 1188, 1204
Ppu21I YACGTR 1 cut(s) 959
PshAI GACNNNNGTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 1068
PspCI CACGTG 1 cut(s) 959
PspEI GGTNACC 1 cut(s) 333
PspGI CCWGG 1 cut(s) 1068
PspN4I GGNNCC 3 cut(s) 539, 732, 1130
PspPI GGNCC 1 cut(s) 1129
RsaI GTAC 3 cut(s) 70, 657, 828
RsaNI GTAC 3 cut(s) 69, 656, 827
RseI CAYNNNNRTG 3 cut(s) 95, 623, 957
SaqAI TTAA 5 cut(s) 255, 375, 543, 744, 1020
SatI GCNGC 7 cut(s) 182, 224, 360, 874, 1080, 1083, 1146
Sau96I GGNCC 1 cut(s) 1129
SchI GAGTC 5 cut(s) 134, 942, 1100, 1189, 1205
ScrFI CCNGG 2 cut(s) 134, 1070
SduI GDGCHC 3 cut(s) 44, 859, 954
SfaNI GCATC 6 cut(s) 441, 476, 764, 931, 1126, 1132
SfcI CTRYAG 1 cut(s) 211
SmiMI CAYNNNNRTG 3 cut(s) 95, 623, 957
SmlI CTYRAG 3 cut(s) 305, 542, 647
SmoI CTYRAG 3 cut(s) 305, 542, 647
SphI GCATGC 1 cut(s) 797
Sse9I AATT 5 cut(s) 555, 693, 714, 741, 822
StyD4I CCNGG 2 cut(s) 132, 1068
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 5 cut(s) 352, 373, 418, 724, 1108
TaiI ACGT 2 cut(s) 961, 1120
TasI AATT 5 cut(s) 555, 693, 714, 741, 822
TatI WGTACW 1 cut(s) 655
Tru1I TTAA 5 cut(s) 255, 375, 543, 744, 1020
Tru9I TTAA 5 cut(s) 255, 375, 543, 744, 1020
TscAI CASTG 2 cut(s) 376, 989
TseFI GTSAC 2 cut(s) 333, 601
TseI GCWGC 7 cut(s) 181, 223, 359, 873, 1079, 1082, 1145
Tsp45I GTSAC 2 cut(s) 333, 601
TspDTI ATGAA 8 cut(s) 113, 218, 567, 607, 656, 878, 903, 968
TspGWI ACGGA 2 cut(s) 34, 56
TspRI CASTG 2 cut(s) 376, 989
Vha464I CTTAAG 1 cut(s) 542
VneI GTGCAC 1 cut(s) 855
XceI RCATGY 3 cut(s) 468, 797, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.