MD07G1199600.v1.1

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010937
Physical Location & Seq
Forward (+)
27787236 .. 27790287
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1199600.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGATGACAGCCTCATCAACATCGCTAATTCAGCGCGTCTGCGCCGTTCCTCTCGCACGCGCGGCCCGCACTAGTGCCATGGTAGCGGCGAGCATGAACATGTCCACCACCTCCACCACCACCACCACCACTACCACCGCTCCCTCCCTAGCCAAACGGGCCGACCCCCAAGTCCTCCTAGGCTTGTCGGAACCGGAGCTCCAGCAGCTCGCAATCGACTTCAGTCAGCAAGGCTACAGAGGGAAGCAGCTCCATCATCTTATTTACAAAAGAAAGATTAAAGATATTCAGGACTTCAATCAGGTGCCTCTGGCATTCAGAAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGGAGGTCACCCATTTACCAGAGCGTCACTGCTGCTGATGGCACTGTCAAGTTGTTGATAAAGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGATGAGAAAGGTGTGATGCGCCTTACAGCGTGTGTCTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGTGCCACGGGAAAAGGAGGATTTTCAAGAAACCTCAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAAATCTTCAACCATAGGGTGACTAATGTGGTGTTCATGGGAATGGGTGAACCAATGATGAACCTGAAGGCGGTTCTTGAAGCACATCAGTGTTTAAACAAGGATATTCAAATTGGGCAAAGAATGATCACAATTTCTACAGTGGGGGTTCCGAATACAATTAAAAAGCTGGCATCTCACAAACTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCCAACCAGAAGCTGAGGGAAACAATTGTGCCAAGTGCAAAAGCATACCCTCTGAGTGCACTGATGAGGGATTGCAGGGACTATTTCGCTGAAACCGGTAGACGGGTTTCCCTTGAGTATGCACTCTTAGCTGGAGTCAATGATGCAGTAGACCACGCAGTAGAACTTGCAGAGCTACTCCATGAGTGGGGACGAGGTTCTCATGTGAACCTGATACCATTTAATCCAATAGAAGGCTCTGAGTATAAACGTCCGTCCAAGAAAGCAGTACAAGCATTTGCAGCTGCTTTGGAGAACAATAAAGTAACGGTCAGCACACGTCAAACAAGGGGCTTGGATGCAGATGCAGCTTGTGGTCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGCCCGTTGACTCTGATAACTTTCAAACTGATGAAGATGCTGCAGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

424

Amino Acids

46.31

Weight (kDa)

8.96

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 166 - 329 1e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 140
AccI GTMKAC 3 cut(s) 797, 922, 972
AccII CGCG 3 cut(s) 36, 60, 62
AciI CCGC 5 cut(s) 62, 67, 86, 138, 677
AcuI CTGAAG 3 cut(s) 205, 692, 776
AfaI GTAC 1 cut(s) 1092
AfiI CCNNNNNNNGG 4 cut(s) 676, 924, 1009, 1055
AflIII ACRYGT 2 cut(s) 99, 1139
AgeI ACCGGT 1 cut(s) 917
AgsI TTSAA 7 cut(s) 298, 437, 558, 616, 686, 716, 1235
AhlI ACTAGT 1 cut(s) 71
AjiI CACGTC 1 cut(s) 1142
AleI CACNNNNGTG 1 cut(s) 694
AloI GAACNNNNNNTCC 2 cut(s) 183, 215
Alw21I GWGCWC 2 cut(s) 201, 883
Alw26I GTCTC 1 cut(s) 499
Alw44I GTGCAC 1 cut(s) 879
AlwNI CAGNNNCTG 1 cut(s) 835
AoxI GGCC 2 cut(s) 63, 159
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 7 cut(s) 205, 247, 383, 1103, 1106, 1169, 1250
AsiGI ACCGGT 1 cut(s) 917
AspA2I CCTAGG 1 cut(s) 178
AspLEI GCGC 4 cut(s) 36, 44, 62, 480
AspS9I GGNCC 2 cut(s) 64, 159
AsuHPI GGTGA 3 cut(s) 351, 637, 665
AvrII CCTAGG 1 cut(s) 178
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 2 cut(s) 201, 883
BbvCI CCTCAGC 1 cut(s) 836
BbvI GCAGC 7 cut(s) 217, 259, 370, 1093, 1115, 1181, 1237
BccI CCATC 3 cut(s) 261, 336, 383
BceAI ACGGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 732
BcoDI GTCTC 1 cut(s) 499
BcuI ACTAGT 1 cut(s) 71
BfaI CTAG 3 cut(s) 72, 149, 179
BfmI CTRYAG 3 cut(s) 235, 744, 1251
BfuAI ACCTGC 1 cut(s) 580
BglI GCCNNNNNGGC 1 cut(s) 158
BisI GCNGC 9 cut(s) 63, 87, 206, 248, 384, 1104, 1107, 1170, 1251
BlnI CCTAGG 1 cut(s) 178
BlsI GCNGC 9 cut(s) 64, 88, 207, 249, 385, 1105, 1108, 1171, 1252
BmgBI CACGTC 1 cut(s) 1142
BmgT120I GGNCC 2 cut(s) 64, 159
BmiI GGNNCC 3 cut(s) 192, 306, 756
BmsI GCATC 7 cut(s) 465, 500, 788, 955, 1150, 1156, 1237
BoxI GACNNNNGTC 1 cut(s) 222
BpmI CTGGAG 2 cut(s) 185, 975
Bpu10I CCTNAGC 1 cut(s) 836
BpuEI CTTGAG 3 cut(s) 350, 551, 956
BsaJI CCNNGG 3 cut(s) 78, 178, 537
BsaWI WCCGGW 2 cut(s) 193, 917
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 183, 213
Bsc4I CCNNNNNNNGG 4 cut(s) 676, 924, 1009, 1055
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 1 cut(s) 452
BseDI CCNNGG 3 cut(s) 78, 178, 537
BseGI GGATG 3 cut(s) 347, 515, 1165
BseLI CCNNNNNNNGG 4 cut(s) 676, 924, 1009, 1055
BseMII CTCAG 3 cut(s) 827, 866, 1053
BseNI ACTGG 1 cut(s) 452
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 7 cut(s) 217, 259, 370, 1093, 1115, 1181, 1237
Bsh1236I CGCG 3 cut(s) 36, 60, 62
BshFI GGCC 2 cut(s) 65, 161
BshNI GGYRCC 1 cut(s) 304
BshTI ACCGGT 1 cut(s) 917
BsiHKAI GWGCWC 2 cut(s) 201, 883
BsiSI CCGG 2 cut(s) 194, 918
BslFI GGGAC 2 cut(s) 914, 1026
BslI CCNNNNNNNGG 4 cut(s) 676, 924, 1009, 1055
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 2 cut(s) 914, 1026
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 2 cut(s) 65, 161
Bsp1286I GDGCHC 2 cut(s) 201, 883
Bsp143I GATC 1 cut(s) 732
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 5 cut(s) 62, 67, 86, 138, 677
BspANI GGCC 2 cut(s) 65, 161
BspCNI CTCAG 3 cut(s) 828, 867, 1054
BspFNI CGCG 3 cut(s) 36, 60, 62
BspLI GGNNCC 3 cut(s) 192, 306, 756
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 304
BsrBI CCGCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 917
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 3 cut(s) 78, 178, 537
BssMI GATC 1 cut(s) 732
BssT1I CCWWGG 2 cut(s) 78, 178
Bst4CI ACNGT 4 cut(s) 397, 442, 748, 1132
Bst6I CTCTTC 2 cut(s) 522, 1196
BstC8I GCNNGC 7 cut(s) 58, 67, 91, 210, 777, 815, 819
BstDEI CTNAG 5 cut(s) 836, 875, 949, 1062, 1184
BstDSI CCRYGG 2 cut(s) 78, 537
BstEII GGTNACC 1 cut(s) 357
BstF5I GGATG 3 cut(s) 347, 515, 1165
BstFNI CGCG 3 cut(s) 36, 60, 62
BstHHI GCGC 4 cut(s) 36, 44, 62, 480
BstKTI GATC 1 cut(s) 735
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 1 cut(s) 732
BstNSI RCATGY 3 cut(s) 103, 821, 1269
BstPAI GACNNNNGTC 1 cut(s) 222
BstPI GGTNACC 1 cut(s) 357
BstSFI CTRYAG 3 cut(s) 235, 744, 1251
BstSLI GKGCMC 1 cut(s) 883
BstUI CGCG 3 cut(s) 36, 60, 62
BstV1I GCAGC 7 cut(s) 217, 259, 370, 1093, 1115, 1181, 1237
BsuRI GGCC 2 cut(s) 65, 161
BtgI CCRYGG 2 cut(s) 78, 537
BtgZI GCGATG 1 cut(s) 6
BtrI CACGTC 1 cut(s) 1142
BtsCI GGATG 3 cut(s) 347, 515, 1165
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 5 cut(s) 378, 393, 701, 753, 881
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 7 cut(s) 58, 67, 91, 210, 777, 815, 819
CaiI CAGNNNCTG 1 cut(s) 835
CfoI GCGC 4 cut(s) 36, 44, 62, 480
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 2 cut(s) 64, 159
CseI GACGC 2 cut(s) 25, 364
Csp6I GTAC 1 cut(s) 1091
CspAI ACCGGT 1 cut(s) 917
CviAII CATG 9 cut(s) 79, 94, 100, 575, 643, 818, 1004, 1025, 1266
CviQI GTAC 1 cut(s) 1091
DdeI CTNAG 5 cut(s) 836, 875, 949, 1062, 1184
DpnI GATC 1 cut(s) 734
DpnII GATC 1 cut(s) 732
DraI TTTAAA 1 cut(s) 702
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 2 cut(s) 522, 1196
EarI CTCTTC 2 cut(s) 522, 1196
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 78, 178
Eco24I GRGCYC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 3 cut(s) 205, 692, 776
Eco91I GGTNACC 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 357
EcoT14I CCWWGG 2 cut(s) 78, 178
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 78, 178
FaeI CATG 9 cut(s) 82, 97, 103, 578, 646, 821, 1007, 1028, 1269
FaqI GGGAC 2 cut(s) 914, 1026
FatI CATG 9 cut(s) 78, 93, 99, 574, 642, 817, 1003, 1024, 1265
FauI CCCGC 1 cut(s) 74
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 3 cut(s) 797, 922, 972
Fnu4HI GCNGC 9 cut(s) 63, 87, 206, 248, 384, 1104, 1107, 1170, 1251
FokI GGATG 3 cut(s) 354, 522, 1172
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 9 cut(s) 63, 87, 206, 248, 384, 1104, 1107, 1170, 1251
FspBI CTAG 3 cut(s) 72, 149, 179
GlaI GCGC 4 cut(s) 35, 43, 61, 479
GluI GCNGC 9 cut(s) 63, 87, 206, 248, 384, 1104, 1107, 1170, 1251
GsuI CTGGAG 2 cut(s) 185, 975
HaeIII GGCC 2 cut(s) 65, 161
HapII CCGG 2 cut(s) 194, 918
HgaI GACGC 2 cut(s) 25, 364
HhaI GCGC 4 cut(s) 36, 44, 62, 480
Hin1II CATG 9 cut(s) 82, 97, 103, 578, 646, 821, 1007, 1028, 1269
Hin6I GCGC 4 cut(s) 34, 42, 60, 478
HinP1I GCGC 4 cut(s) 34, 42, 60, 478
HincII GTYRAC 2 cut(s) 798, 1219
HindII GTYRAC 2 cut(s) 798, 1219
HinfI GANTC 3 cut(s) 957, 1204, 1220
HpaII CCGG 2 cut(s) 194, 918
HphI GGTGA 3 cut(s) 351, 637, 665
Hpy166II GTNNAC 8 cut(s) 105, 656, 798, 881, 923, 973, 1030, 1219
Hpy188I TCNGA 6 cut(s) 190, 320, 759, 876, 1063, 1225
Hpy188III TCNNGA 5 cut(s) 290, 558, 568, 683, 1198
Hpy8I GTNNAC 8 cut(s) 105, 656, 798, 881, 923, 973, 1030, 1219
HpyAV CCTTC 2 cut(s) 667, 1049
HpyCH4III ACNGT 4 cut(s) 397, 442, 748, 1132
HpyCH4IV ACGT 2 cut(s) 1072, 1141
HpyF3I CTNAG 5 cut(s) 836, 875, 949, 1062, 1184
HpySE526I ACGT 2 cut(s) 1072, 1141
Hsp92II CATG 9 cut(s) 82, 97, 103, 578, 646, 821, 1007, 1028, 1269
HspAI GCGC 4 cut(s) 34, 42, 60, 478
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 1 cut(s) 732
LmnI GCTCC 5 cut(s) 145, 196, 204, 255, 826
Lsp1109I GCAGC 7 cut(s) 217, 259, 370, 1093, 1115, 1181, 1237
LweI GCATC 7 cut(s) 465, 500, 788, 955, 1150, 1156, 1237
MaeI CTAG 3 cut(s) 72, 149, 179
MaeII ACGT 2 cut(s) 1072, 1141
MaeIII GTNAC 4 cut(s) 357, 376, 625, 1126
MalI GATC 1 cut(s) 734
MbiI CCGCTC 1 cut(s) 140
MboI GATC 1 cut(s) 732
MboII GAAGA 4 cut(s) 509, 604, 1213, 1256
MfeI CAATTG 1 cut(s) 846
MhlI GDGCHC 2 cut(s) 201, 883
MluCI AATT 6 cut(s) 27, 579, 717, 738, 765, 846
MlyI GAGTC 3 cut(s) 966, 1213, 1214
MmeI TCCRAC 2 cut(s) 168, 396
MseI TTAA 5 cut(s) 279, 456, 701, 768, 1044
MslI CAYNNNNRTG 3 cut(s) 98, 647, 694
MspA1I CMGCKG 1 cut(s) 1106
MspI CCGG 2 cut(s) 194, 918
MssI GTTTAAAC 1 cut(s) 702
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 314
MvnI CGCG 3 cut(s) 36, 60, 62
NcoI CCATGG 1 cut(s) 78
NdeII GATC 1 cut(s) 732
NlaIII CATG 9 cut(s) 82, 97, 103, 578, 646, 821, 1007, 1028, 1269
NlaIV GGNNCC 3 cut(s) 192, 306, 756
NmuCI GTSAC 3 cut(s) 357, 376, 625
NspI RCATGY 3 cut(s) 103, 821, 1269
OliI CACNNNNGTG 1 cut(s) 694
PaeI GCATGC 1 cut(s) 821
PciI ACATGT 1 cut(s) 99
PctI GAATGC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 917
PkrI GCNGC 9 cut(s) 64, 88, 207, 249, 385, 1105, 1108, 1171, 1252
PleI GAGTC 3 cut(s) 965, 1212, 1214
PmeI GTTTAAAC 1 cut(s) 702
PpsI GAGTC 3 cut(s) 965, 1212, 1214
PscI ACATGT 1 cut(s) 99
PshAI GACNNNNGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 357
PspN4I GGNNCC 3 cut(s) 192, 306, 756
PspPI GGNCC 2 cut(s) 64, 159
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 1 cut(s) 835
PvuII CAGCTG 1 cut(s) 1106
RsaI GTAC 1 cut(s) 1092
RsaNI GTAC 1 cut(s) 1091
RseI CAYNNNNRTG 3 cut(s) 98, 647, 694
SacI GAGCTC 1 cut(s) 201
SalI GTCGAC 1 cut(s) 796
SaqAI TTAA 5 cut(s) 279, 456, 701, 768, 1044
SatI GCNGC 9 cut(s) 63, 87, 206, 248, 384, 1104, 1107, 1170, 1251
Sau3AI GATC 1 cut(s) 732
Sau96I GGNCC 2 cut(s) 64, 159
SchI GAGTC 3 cut(s) 966, 1213, 1214
SduI GDGCHC 2 cut(s) 201, 883
SfaNI GCATC 7 cut(s) 465, 500, 788, 955, 1150, 1156, 1237
SfcI CTRYAG 3 cut(s) 235, 744, 1251
SmiMI CAYNNNNRTG 3 cut(s) 98, 647, 694
SmlI CTYRAG 3 cut(s) 329, 566, 935
SmoI CTYRAG 3 cut(s) 329, 566, 935
SpeI ACTAGT 1 cut(s) 71
SphI GCATGC 1 cut(s) 821
Sse9I AATT 6 cut(s) 27, 579, 717, 738, 765, 846
SsiI CCGC 5 cut(s) 62, 67, 86, 138, 677
SspMI CTAG 3 cut(s) 72, 149, 179
SstI GAGCTC 1 cut(s) 201
StyI CCWWGG 2 cut(s) 78, 178
TaaI ACNGT 4 cut(s) 397, 442, 748, 1132
TaiI ACGT 2 cut(s) 1075, 1144
TaqI TCGA 2 cut(s) 216, 797
TasI AATT 6 cut(s) 27, 579, 717, 738, 765, 846
TatI WGTACW 1 cut(s) 1090
TauI GCSGC 2 cut(s) 65, 89
Tru1I TTAA 5 cut(s) 279, 456, 701, 768, 1044
Tru9I TTAA 5 cut(s) 279, 456, 701, 768, 1044
TscAI CASTG 5 cut(s) 385, 400, 701, 753, 888
TseFI GTSAC 3 cut(s) 357, 376, 625
TseI GCWGC 7 cut(s) 205, 247, 383, 1103, 1106, 1169, 1250
Tsp45I GTSAC 3 cut(s) 357, 376, 625
TspDTI ATGAA 5 cut(s) 110, 591, 631, 680, 1257
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 5 cut(s) 385, 400, 701, 753, 888
VneI GTGCAC 1 cut(s) 879
XceI RCATGY 3 cut(s) 103, 821, 1269
XmaJI CCTAGG 1 cut(s) 178
XmiI GTMKAC 3 cut(s) 797, 922, 972
XspI CTAG 3 cut(s) 72, 149, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.