pycom07g18640

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010937
Physical Location & Seq
Forward (+)
21087210 .. 21089845
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g18640.10

Sequence Viewer

Length: 1365 bp
ATGGGATCGATCCAACTTGGGCCTAAACCACACCACGCTCTTTCTTCCTCTGCTTCATCTTCTTCATCTCGCGCGAAGCCAAAAACCCAATACAGCACAATGATGACAGCCTCATCAACATCGCTAATTCAGCGCGTCTGCGCCGTTCCTCTCGCACGCGCGGCCCGCACTCGCGCCATGGCAGCGGCGAGCATGAACCTGTCCACCACCACCTCCACCACCACCACCACCGCTCCCTCCCTAGCGAAACGGGCCGACCCCCAAGTCCTCCTAGGCTTGTCGGAACCGGAGCTCCAGCAGCTCGCAATCGACTTCAGTCAGCAAGGCTACAGAGGGAAGCAGCTCCATCATCTAATTTACAAAAGAAAGATTAAAGATATTCAGGATTTCAATCAGGTGCCTCTGGCATTCAGAAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGGAGGTCACCCATTTACCAGAGCGTCACTGCTGCTGATGGCACTGTCAAGTTGTTGATAAAGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGATGAGAAAGGTGTGATGCGCCTTACAGCGTGTGTCTCATCACAGGTGGGATGCCCTCTTCGTTGCTCTTTTTGTGCCACGGGAAAAGGAGGATTTTCAAGAAACCTCAAGAGGCATGAAATTGTTGAGCAGGTTTTGGCTATTGAGGAAATCTTCAACCATAGGGTGACTAATGTGGTGTTCATGGGAATGGGTGAACCGATGATGAACCTGAAGGCGGTTCTTGAAGCGCATCAGTGTTTAAACAAGGATATTCAAATTGGGCAAAGAATGATCACAATTTCTACGGTGGGGGTTCCAAATACAATTAAAAAGCTGGCATCCCACAAACTTCAGTCGACATTGGCTGTCAGCCTGCATGCTCCTAACCAGAAGCTGAGGGAAACAATTGTGCCAAGTGCAAAAGCATACCCTCTGAGTGCACTGATGAGGGATTGCAGGGACTATTTCACTGAAACCGGTAGACGGGTTTCCTTTGAGTATGCACTCTTAGCTGGAGTCAATGATGCGGTAGACCACGCAGTAGAACTTGCAGAGCTACTCCATGAGTGGGGACGAGGTTCTCATGTGAACCTGATACCATTTAATCCAATAGAAGGCTCTGAGTATAAACGTCCATCCAAGAAAGCGGTACAAGCATTTGCAGCTGCTCTGGAGAACAATAAAGTAACGGTCAGCACACGTCAAACAAGGGGCTTGGATGCAGATGCAGCTTGTGGTCAGCTAAGAAACAAGTTCCAGAAGAGTCCTTTGCCCGTTGACTCTGATAACTTACAAACTGATGAAGATGCTCCAGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

455

Amino Acids

49.59

Weight (kDa)

9.3

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 233
AccI GTMKAC 3 cut(s) 890, 1015, 1065
AccII CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
AciI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
AclWI GGATC 2 cut(s) 4, 13
AcuI CTGAAG 3 cut(s) 298, 785, 869
AfaI GTAC 1 cut(s) 1185
AfiI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
AflIII ACRYGT 1 cut(s) 1232
AgeI ACCGGT 1 cut(s) 1010
AgsI TTSAA 6 cut(s) 391, 530, 651, 709, 779, 809
AjiI CACGTC 1 cut(s) 1235
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
Alw21I GWGCWC 2 cut(s) 294, 976
Alw26I GTCTC 1 cut(s) 592
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 2 cut(s) 4, 13
AlwNI CAGNNNCTG 1 cut(s) 928
AoxI GGCC 3 cut(s) 20, 162, 252
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 7 cut(s) 182, 298, 340, 476, 1196, 1199, 1262
AsiGI ACCGGT 1 cut(s) 1010
AspA2I CCTAGG 1 cut(s) 271
AspLEI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
AspS9I GGNCC 3 cut(s) 20, 163, 252
AsuHPI GGTGA 3 cut(s) 444, 730, 758
AvrII CCTAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 976
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 294, 976
BbvCI CCTCAGC 1 cut(s) 929
BbvI GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
BccI CCATC 4 cut(s) 354, 429, 476, 1177
BceAI ACGGC 1 cut(s) 128
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 2 cut(s) 242, 272
BfmI CTRYAG 1 cut(s) 328
BfuAI ACCTGC 1 cut(s) 673
BisI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
BlnI CCTAGG 1 cut(s) 271
BlsI GCNGC 9 cut(s) 163, 184, 187, 300, 342, 478, 1198, 1201, 1264
BmgBI CACGTC 1 cut(s) 1235
BmgT120I GGNCC 3 cut(s) 20, 163, 252
BmiI GGNNCC 3 cut(s) 285, 399, 849
BmsI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
BoxI GACNNNNGTC 1 cut(s) 315
BpmI CTGGAG 4 cut(s) 278, 1068, 1226, 1329
Bpu10I CCTNAGC 1 cut(s) 929
BpuEI CTTGAG 2 cut(s) 443, 644
Bsa29I ATCGAT 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 177, 271, 630
BsaWI WCCGGW 2 cut(s) 286, 1010
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 276, 306
Bsc4I CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 2 cut(s) 545, 1346
Bse8I GATNNNNATC 1 cut(s) 390
BseCI ATCGAT 1 cut(s) 8
BseDI CCNNGG 3 cut(s) 177, 271, 630
BseGI GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
BseMII CTCAG 3 cut(s) 920, 959, 1146
BseNI ACTGG 2 cut(s) 545, 1346
BseSI GKGCMC 1 cut(s) 976
BseXI GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
Bsh1236I CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BshFI GGCC 3 cut(s) 22, 164, 254
BshNI GGYRCC 1 cut(s) 397
BshTI ACCGGT 1 cut(s) 1010
BshVI ATCGAT 1 cut(s) 8
BsiHKAI GWGCWC 2 cut(s) 294, 976
BsiSI CCGG 2 cut(s) 287, 1011
BslFI GGGAC 2 cut(s) 1007, 1119
BslI CCNNNNNNNGG 4 cut(s) 769, 1017, 1102, 1148
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 2 cut(s) 1007, 1119
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 3 cut(s) 22, 164, 254
Bsp1286I GDGCHC 2 cut(s) 294, 976
Bsp143I GATC 3 cut(s) 5, 9, 825
Bsp19I CCATGG 1 cut(s) 177
BspACI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
BspANI GGCC 3 cut(s) 22, 164, 254
BspCNI CTCAG 3 cut(s) 921, 960, 1147
BspDI ATCGAT 1 cut(s) 8
BspFNI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BspLI GGNNCC 3 cut(s) 285, 399, 849
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 2 cut(s) 4, 13
BspT107I GGYRCC 1 cut(s) 397
BsrBI CCGCTC 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 2 cut(s) 545, 1346
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 3 cut(s) 177, 271, 630
BssMI GATC 3 cut(s) 5, 9, 825
BssT1I CCWWGG 2 cut(s) 177, 271
Bst4CI ACNGT 4 cut(s) 490, 535, 841, 1225
Bst6I CTCTTC 2 cut(s) 615, 1289
BstC8I GCNNGC 7 cut(s) 157, 166, 190, 303, 870, 908, 912
BstDEI CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
BstDSI CCRYGG 2 cut(s) 177, 630
BstEII GGTNACC 1 cut(s) 450
BstF5I GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BstFNI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BstHHI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
BstKTI GATC 3 cut(s) 8, 12, 828
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 3 cut(s) 5, 9, 825
BstNSI RCATGY 2 cut(s) 914, 1362
BstPAI GACNNNNGTC 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 450
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
BstV1I GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
Bsu15I ATCGAT 1 cut(s) 8
BsuRI GGCC 3 cut(s) 22, 164, 254
BsuTUI ATCGAT 1 cut(s) 8
BtgI CCRYGG 2 cut(s) 177, 630
BtgZI GCGATG 1 cut(s) 105
BtrI CACGTC 1 cut(s) 1235
BtsCI GGATG 5 cut(s) 440, 608, 872, 1169, 1258
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 5 cut(s) 471, 486, 794, 974, 1002
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 7 cut(s) 157, 166, 190, 303, 870, 908, 912
CaiI CAGNNNCTG 1 cut(s) 928
CfoI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 3 cut(s) 20, 163, 252
ClaI ATCGAT 1 cut(s) 8
CseI GACGC 2 cut(s) 124, 457
Csp6I GTAC 1 cut(s) 1184
CspAI ACCGGT 1 cut(s) 1010
CviAII CATG 8 cut(s) 178, 193, 668, 736, 911, 1097, 1118, 1359
CviQI GTAC 1 cut(s) 1184
DdeI CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
DpnI GATC 3 cut(s) 7, 11, 827
DpnII GATC 3 cut(s) 5, 9, 825
DraI TTTAAA 1 cut(s) 795
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 2 cut(s) 615, 1289
EarI CTCTTC 2 cut(s) 615, 1289
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 177, 271
Eco24I GRGCYC 1 cut(s) 294
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 3 cut(s) 298, 785, 869
Eco91I GGTNACC 1 cut(s) 450
EcoICRI GAGCTC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 177, 271
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 177, 271
FaeI CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
FaqI GGGAC 2 cut(s) 1007, 1119
FatI CATG 8 cut(s) 177, 192, 667, 735, 910, 1096, 1117, 1358
FauI CCCGC 1 cut(s) 173
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 3 cut(s) 890, 1015, 1065
Fnu4HI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
FokI GGATG 5 cut(s) 447, 615, 859, 1156, 1265
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
FspBI CTAG 2 cut(s) 242, 272
GlaI GCGC 7 cut(s) 73, 134, 142, 160, 175, 572, 783
GluI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
GsuI CTGGAG 4 cut(s) 278, 1068, 1226, 1329
HaeIII GGCC 3 cut(s) 22, 164, 254
HapII CCGG 2 cut(s) 287, 1011
HgaI GACGC 2 cut(s) 124, 457
HhaI GCGC 7 cut(s) 74, 135, 143, 161, 176, 573, 784
Hin1II CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
Hin6I GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
HinP1I GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
HincII GTYRAC 2 cut(s) 891, 1312
HindII GTYRAC 2 cut(s) 891, 1312
HinfI GANTC 3 cut(s) 1050, 1297, 1313
HpaII CCGG 2 cut(s) 287, 1011
HphI GGTGA 3 cut(s) 444, 730, 758
Hpy166II GTNNAC 8 cut(s) 204, 749, 891, 974, 1016, 1066, 1123, 1312
Hpy188I TCNGA 5 cut(s) 283, 413, 969, 1156, 1318
Hpy188III TCNNGA 6 cut(s) 383, 651, 661, 776, 1205, 1291
Hpy8I GTNNAC 8 cut(s) 204, 749, 891, 974, 1016, 1066, 1123, 1312
HpyAV CCTTC 2 cut(s) 760, 1142
HpyCH4III ACNGT 4 cut(s) 490, 535, 841, 1225
HpyCH4IV ACGT 2 cut(s) 1165, 1234
HpyF3I CTNAG 5 cut(s) 929, 968, 1042, 1155, 1277
HpySE526I ACGT 2 cut(s) 1165, 1234
Hsp92II CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
HspAI GCGC 7 cut(s) 72, 133, 141, 159, 174, 571, 782
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 3 cut(s) 5, 9, 825
LmnI GCTCC 6 cut(s) 238, 289, 297, 348, 919, 1348
Lsp1109I GCAGC 7 cut(s) 194, 310, 352, 463, 1186, 1208, 1274
LweI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
MaeI CTAG 2 cut(s) 242, 272
MaeII ACGT 2 cut(s) 1165, 1234
MaeIII GTNAC 4 cut(s) 450, 469, 718, 1219
MalI GATC 3 cut(s) 7, 11, 827
MbiI CCGCTC 1 cut(s) 233
MboI GATC 3 cut(s) 5, 9, 825
MboII GAAGA 7 cut(s) 36, 51, 54, 602, 697, 1306, 1349
MfeI CAATTG 1 cut(s) 939
MhlI GDGCHC 2 cut(s) 294, 976
MluCI AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
MlyI GAGTC 3 cut(s) 1059, 1306, 1307
MmeI TCCRAC 3 cut(s) 37, 261, 489
MseI TTAA 5 cut(s) 372, 549, 794, 861, 1137
MslI CAYNNNNRTG 2 cut(s) 101, 740
MspA1I CMGCKG 2 cut(s) 185, 1199
MspI CCGG 2 cut(s) 287, 1011
MssI GTTTAAAC 1 cut(s) 795
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 407
MvnI CGCG 6 cut(s) 72, 74, 135, 159, 161, 174
NcoI CCATGG 1 cut(s) 177
NdeII GATC 3 cut(s) 5, 9, 825
NlaIII CATG 8 cut(s) 181, 196, 671, 739, 914, 1100, 1121, 1362
NlaIV GGNNCC 3 cut(s) 285, 399, 849
NmuCI GTSAC 3 cut(s) 450, 469, 718
NspI RCATGY 2 cut(s) 914, 1362
PaeI GCATGC 1 cut(s) 914
PctI GAATGC 1 cut(s) 407
PinAI ACCGGT 1 cut(s) 1010
PkrI GCNGC 9 cut(s) 163, 184, 187, 300, 342, 478, 1198, 1201, 1264
PleI GAGTC 3 cut(s) 1058, 1305, 1307
PmeI GTTTAAAC 1 cut(s) 795
PpsI GAGTC 3 cut(s) 1058, 1305, 1307
PshAI GACNNNNGTC 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 450
PspN4I GGNNCC 3 cut(s) 285, 399, 849
PspPI GGNCC 3 cut(s) 20, 163, 252
PstNI CAGNNNCTG 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 1 cut(s) 1185
RsaNI GTAC 1 cut(s) 1184
RseI CAYNNNNRTG 2 cut(s) 101, 740
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 889
SaqAI TTAA 5 cut(s) 372, 549, 794, 861, 1137
SatI GCNGC 9 cut(s) 162, 183, 186, 299, 341, 477, 1197, 1200, 1263
Sau3AI GATC 3 cut(s) 5, 9, 825
Sau96I GGNCC 3 cut(s) 20, 163, 252
SchI GAGTC 3 cut(s) 1059, 1306, 1307
SduI GDGCHC 2 cut(s) 294, 976
SfaNI GCATC 8 cut(s) 558, 593, 793, 881, 1048, 1243, 1249, 1330
SfcI CTRYAG 1 cut(s) 328
SmiMI CAYNNNNRTG 2 cut(s) 101, 740
SmlI CTYRAG 2 cut(s) 422, 659
SmoI CTYRAG 2 cut(s) 422, 659
SphI GCATGC 1 cut(s) 914
Sse9I AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
SsiI CCGC 7 cut(s) 161, 166, 185, 231, 770, 1061, 1181
SspMI CTAG 2 cut(s) 242, 272
SstI GAGCTC 1 cut(s) 294
StyI CCWWGG 2 cut(s) 177, 271
TaaI ACNGT 4 cut(s) 490, 535, 841, 1225
TaiI ACGT 2 cut(s) 1168, 1237
TaqI TCGA 3 cut(s) 8, 309, 890
TasI AATT 7 cut(s) 126, 354, 672, 810, 831, 858, 939
TauI GCSGC 2 cut(s) 164, 188
Tru1I TTAA 5 cut(s) 372, 549, 794, 861, 1137
Tru9I TTAA 5 cut(s) 372, 549, 794, 861, 1137
TscAI CASTG 5 cut(s) 478, 493, 794, 981, 1009
TseFI GTSAC 3 cut(s) 450, 469, 718
TseI GCWGC 7 cut(s) 182, 298, 340, 476, 1196, 1199, 1262
Tsp45I GTSAC 3 cut(s) 450, 469, 718
TspDTI ATGAA 7 cut(s) 45, 54, 209, 684, 724, 773, 1350
TspRI CASTG 5 cut(s) 478, 493, 794, 981, 1009
VneI GTGCAC 1 cut(s) 972
XceI RCATGY 2 cut(s) 914, 1362
XmaJI CCTAGG 1 cut(s) 271
XmiI GTMKAC 3 cut(s) 890, 1015, 1065
XspI CTAG 2 cut(s) 242, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.