Prupe.2G236300_v2.0.a1

Dual-specificity RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010937
Physical Location & Seq
Forward (+)
25793596 .. 25796944
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G236300.1

Sequence Viewer

Length: 1242 bp
ATGATGACAGCCACATCAACGTCGCTAATTCAGCGCGTGTGCTCTGTGCCTCTCGCACGTGCGGTCCGTACTCGCGCCATGGCAGTGGTGAGCATGAACCTGTCCACCACTCCAGCTCGTTCTCAAGCCTTGCGCGCCGACTCGCGCGTGCTCCTCGGCTTGTCCGAACCAGAGCTCCAGCAGCTCGCACTGGACTTAAAGCAGCAAAGCTACAGAGGGAAGCAGCTCCATCATCTTATTTATAAAAGAAAGATTAAAGATATTCAGGATTTCAGTCAAGTGCCTCTGGCATTCAGGAATGAGCTTGAGGAAGCTGGATGGAAAGTTGGGAGGTCACCCATTTACCAGAGTGTCACTGCTGCTGATGGCACTGTTAAGTTATTGATAAAGTTGGAGGATAACAGATTGATTGAAACTGTTGGCATACCAGTTAAAGATGAGAAAGGTGTGATGCGCCTTACAGCATGTGTCTCCTCACAGGTGGGATGCCCACTTCGTTGCTCTTTTTGTGCCACAGGAAAAGGAGGGTTTTCAAGGAACCTTAAGATGCATGAAATTGTTGAGCAGGTTTTGGCTGTTGAGGAAATCTTCAACCATAGGGTGACCAATGTAGTGTTCATGGGAATGGGTGAACCAATGATGAACCTGAAGTCAGTACTTGCAGCACACCAGTGTTTAAATAAGGATGTGCAAATTGGGCAAAGAATGATCACAATTTCTACTGTGGGGGTTCCAAATACAATAAAAAAGCTGGCATCTCACAAGCTTCAGTCAACATTGGCTGTCAGTCTGCATGCTCCTAACCAGAAGCTCAGGGAAACAATTGTGCCAAGTGCAAAAGCATACCCTCTGAGTGCACTTATGAAGGATTGCAGGGACTATTTCGTTGAAACCAGTAGACGGGTTTCCTTTGAGTATGCACTTTTAGCTGGAGTCAATGATGCAGTAGAGCATGCAGTCGAACTTGCAGAGCTACTCCATGAGTGGGGGCGAGGCTATCATGTGAACTTGATACCTTTTAACCCAATAGAAGGCTCTGAGTATCAGCGTCCATCCAAGAAAGCTGTACAGGCATTTGCAGCTGCTCTGGAGTCCAATAGAGTAACTGTTAGCACACGTCAAACAAGGGGCTTGGATGCAGATGCAGCTTGTGGTCAGCTGAGAAACAAGTTCCAGAAGAGTCCTTTGCCCACTGACTCCGACAACTTACAACCTGAAGAAGATGTTGCAGTTGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

414

Amino Acids

45.64

Weight (kDa)

9.13

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39670 AT2G39670
fragaria_vesca FvH4_7g22600
malus_domestica MD07G1012700.v1.1 MD07G1199600.v1.1
prunus_persica Prupe.2G236300_v2.0.a1
pyrus_communis pycom07g01040 pycom07g18640
rosa_chinensis RchiOBHm_Chr1g0367701
rosa_laevigata RLG00000027269
rosa_multiflora Rmu_sc0008470.1_g000011 Rmu_sc0022951.1_g000001 Rmu_sc0027455.1_g000001
rosa_roxburghii Rroxscaffold_4G00289290
rosa_rugosa Rorug01G0336200
rosa_samantha Rh1AG343200 Rh1BG304900 Rh1CG320600 Rh1DG337200
rosa_wichuraiana Rw1G030540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 556
AccI GTMKAC 1 cut(s) 898
AccII CGCG 5 cut(s) 36, 75, 135, 145, 147
AciI CCGC 1 cut(s) 62
AcuI CTGAAG 3 cut(s) 668, 752, 1236
AcvI CACGTG 1 cut(s) 59
AfaI GTAC 3 cut(s) 70, 657, 1068
AfiI CCNNNNNNNGG 3 cut(s) 900, 985, 1031
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 1115
AgsI TTSAA 4 cut(s) 413, 534, 592, 890
AjiI CACGTC 1 cut(s) 1118
AjuI GAANNNNNNNTTGG 2 cut(s) 599, 631
AleI CACNNNNGTG 1 cut(s) 670
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw21I GWGCWC 4 cut(s) 44, 153, 177, 859
Alw26I GTCTC 1 cut(s) 475
Alw44I GTGCAC 1 cut(s) 855
ApaLI GTGCAC 1 cut(s) 855
ApeKI GCWGC 8 cut(s) 181, 202, 223, 359, 662, 1079, 1082, 1145
AspLEI GCGC 6 cut(s) 36, 77, 135, 137, 147, 456
AspS9I GGNCC 1 cut(s) 64
AsuHPI GGTGA 4 cut(s) 100, 327, 613, 641
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 859
BanII GRGCYC 1 cut(s) 177
BbrPI CACGTG 1 cut(s) 59
Bbv12I GWGCWC 4 cut(s) 44, 153, 177, 859
BbvI GCAGC 8 cut(s) 193, 214, 235, 346, 674, 1069, 1091, 1157
BccI CCATC 4 cut(s) 237, 312, 359, 1060
BclI TGATCA 1 cut(s) 708
BcoDI GTCTC 1 cut(s) 475
BfmI CTRYAG 1 cut(s) 211
BfrI CTTAAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 556
BisI GCNGC 8 cut(s) 182, 203, 224, 360, 663, 1080, 1083, 1146
BlsI GCNGC 8 cut(s) 183, 204, 225, 361, 664, 1081, 1084, 1147
BmcAI AGTACT 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 1118
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 539, 732
BmsI GCATC 7 cut(s) 441, 476, 537, 764, 931, 1126, 1132
BpmI CTGGAG 4 cut(s) 96, 161, 951, 1109
Bpu10I CCTNAGC 1 cut(s) 812
BpuEI CTTGAG 2 cut(s) 108, 326
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 78, 154
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 3 cut(s) 900, 985, 1031
Bse1I ACTGG 4 cut(s) 195, 428, 670, 894
BseDI CCNNGG 2 cut(s) 78, 154
BseGI GGATG 5 cut(s) 323, 491, 691, 1052, 1141
BseLI CCNNNNNNNGG 3 cut(s) 900, 985, 1031
BseMII CTCAG 4 cut(s) 826, 842, 1029, 1151
BseNI ACTGG 4 cut(s) 195, 428, 670, 894
BsePI GCGCGC 1 cut(s) 133
BseRI GAGGAG 2 cut(s) 143, 463
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 8 cut(s) 193, 214, 235, 346, 674, 1069, 1091, 1157
Bsh1236I CGCG 5 cut(s) 36, 75, 135, 145, 147
BsiHKAI GWGCWC 4 cut(s) 44, 153, 177, 859
BslFI GGGAC 1 cut(s) 890
BslI CCNNNNNNNGG 3 cut(s) 900, 985, 1031
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 890
BsmI GAATGC 1 cut(s) 290
Bsp1286I GDGCHC 4 cut(s) 44, 153, 177, 859
Bsp1407I TGTACA 1 cut(s) 1066
Bsp143I GATC 1 cut(s) 708
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 1 cut(s) 62
BspCNI CTCAG 4 cut(s) 825, 843, 1030, 1152
BspFNI CGCG 5 cut(s) 36, 75, 135, 145, 147
BspLI GGNNCC 2 cut(s) 539, 732
BspMI ACCTGC 1 cut(s) 556
BspTI CTTAAG 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 1066
BsrI ACTGG 4 cut(s) 195, 428, 670, 894
BssECI CCNNGG 2 cut(s) 78, 154
BssHII GCGCGC 1 cut(s) 133
BssMI GATC 1 cut(s) 708
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 4 cut(s) 373, 418, 724, 1108
Bst6I CTCTTC 1 cut(s) 1172
BstAFI CTTAAG 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 1066
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 6 cut(s) 135, 149, 186, 753, 795, 954
BstDEI CTNAG 4 cut(s) 812, 851, 1038, 1160
BstDSI CCRYGG 1 cut(s) 78
BstEII GGTNACC 2 cut(s) 333, 601
BstF5I GGATG 5 cut(s) 323, 491, 691, 1052, 1141
BstFNI CGCG 5 cut(s) 36, 75, 135, 145, 147
BstHHI GCGC 6 cut(s) 36, 77, 135, 137, 147, 456
BstKTI GATC 1 cut(s) 711
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 1 cut(s) 708
BstMWI GCNNNNNNNGC 8 cut(s) 31, 134, 181, 697, 926, 1070, 1079, 1145
BstNSI RCATGY 4 cut(s) 468, 797, 956, 1239
BstPI GGTNACC 2 cut(s) 333, 601
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 859
BstUI CGCG 5 cut(s) 36, 75, 135, 145, 147
BstV1I GCAGC 8 cut(s) 193, 214, 235, 346, 674, 1069, 1091, 1157
BstXI CCANNNNNNTGG 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 1118
BtsCI GGATG 5 cut(s) 323, 491, 691, 1052, 1141
BtsI GCAGTG 2 cut(s) 90, 354
BtsIMutI CAGTG 6 cut(s) 90, 188, 354, 369, 677, 1191
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 6 cut(s) 135, 149, 186, 753, 795, 954
CfoI GCGC 6 cut(s) 36, 77, 135, 137, 147, 456
Cfr13I GGNCC 1 cut(s) 64
CpoI CGGWCCG 1 cut(s) 64
CseI GACGC 1 cut(s) 1037
Csp6I GTAC 3 cut(s) 69, 656, 1067
CspI CGGWCCG 1 cut(s) 64
CviQI GTAC 3 cut(s) 69, 656, 1067
DdeI CTNAG 4 cut(s) 812, 851, 1038, 1160
DpnI GATC 1 cut(s) 710
DpnII GATC 1 cut(s) 708
DraI TTTAAA 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 3 cut(s) 668, 752, 1236
Eco72I CACGTG 1 cut(s) 59
Eco91I GGTNACC 2 cut(s) 333, 601
EcoICRI GAGCTC 1 cut(s) 175
EcoO65I GGTNACC 2 cut(s) 333, 601
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 78
FaqI GGGAC 1 cut(s) 890
FbaI TGATCA 1 cut(s) 708
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 8 cut(s) 182, 203, 224, 360, 663, 1080, 1083, 1146
FokI GGATG 5 cut(s) 330, 498, 698, 1039, 1148
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 8 cut(s) 182, 203, 224, 360, 663, 1080, 1083, 1146
GlaI GCGC 6 cut(s) 35, 76, 134, 136, 146, 455
GluI GCNGC 8 cut(s) 182, 203, 224, 360, 663, 1080, 1083, 1146
GsuI CTGGAG 4 cut(s) 96, 161, 951, 1109
HgaI GACGC 1 cut(s) 1037
HhaI GCGC 6 cut(s) 36, 77, 135, 137, 147, 456
Hin6I GCGC 6 cut(s) 34, 75, 133, 135, 145, 454
HinP1I GCGC 6 cut(s) 34, 75, 133, 135, 145, 454
HincII GTYRAC 1 cut(s) 774
HindII GTYRAC 1 cut(s) 774
HindIII AAGCTT 1 cut(s) 764
HinfI GANTC 5 cut(s) 140, 933, 1091, 1180, 1196
HphI GGTGA 4 cut(s) 100, 327, 613, 641
Hpy166II GTNNAC 6 cut(s) 105, 632, 774, 857, 899, 1006
Hpy188I TCNGA 4 cut(s) 166, 852, 1039, 1201
Hpy188III TCNNGA 4 cut(s) 266, 295, 1088, 1174
Hpy8I GTNNAC 6 cut(s) 105, 632, 774, 857, 899, 1006
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 859, 1025
HpyCH4III ACNGT 4 cut(s) 373, 418, 724, 1108
HpyCH4IV ACGT 3 cut(s) 20, 58, 1117
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 134, 181, 697, 926, 1070, 1079, 1145
HpyF3I CTNAG 4 cut(s) 812, 851, 1038, 1160
HpySE526I ACGT 3 cut(s) 20, 58, 1117
HspAI GCGC 6 cut(s) 34, 75, 133, 135, 145, 454
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 1 cut(s) 708
LmnI GCTCC 4 cut(s) 156, 180, 231, 802
Lsp1109I GCAGC 8 cut(s) 193, 214, 235, 346, 674, 1069, 1091, 1157
LweI GCATC 7 cut(s) 441, 476, 537, 764, 931, 1126, 1132
MaeII ACGT 3 cut(s) 20, 58, 1117
MaeIII GTNAC 4 cut(s) 333, 352, 601, 1102
MalI GATC 1 cut(s) 710
MboI GATC 1 cut(s) 708
MboII GAAGA 4 cut(s) 580, 1189, 1229, 1232
MfeI CAATTG 1 cut(s) 822
MhlI GDGCHC 4 cut(s) 44, 153, 177, 859
MluCI AATT 5 cut(s) 27, 555, 693, 714, 822
MlyI GAGTC 5 cut(s) 134, 942, 1100, 1189, 1190
MmeI TCCRAC 2 cut(s) 372, 1224
Mph1103I ATGCAT 1 cut(s) 552
MseI TTAA 7 cut(s) 197, 255, 375, 432, 543, 677, 1020
MslI CAYNNNNRTG 3 cut(s) 83, 623, 670
MspA1I CMGCKG 2 cut(s) 1082, 1159
MspCI CTTAAG 1 cut(s) 542
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 1 cut(s) 290
MvnI CGCG 5 cut(s) 36, 75, 135, 145, 147
MwoI GCNNNNNNNGC 8 cut(s) 31, 134, 181, 697, 926, 1070, 1079, 1145
NcoI CCATGG 1 cut(s) 78
NdeII GATC 1 cut(s) 708
NlaIV GGNNCC 2 cut(s) 539, 732
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 3 cut(s) 333, 352, 601
NsiI ATGCAT 1 cut(s) 552
NspI RCATGY 4 cut(s) 468, 797, 956, 1239
OliI CACNNNNGTG 1 cut(s) 670
PaeI GCATGC 2 cut(s) 797, 956
PauI GCGCGC 1 cut(s) 133
PcsI WCGNNNNNNNCGW 2 cut(s) 64, 162
PctI GAATGC 1 cut(s) 290
PkrI GCNGC 8 cut(s) 183, 204, 225, 361, 664, 1081, 1084, 1147
PleI GAGTC 5 cut(s) 134, 941, 1099, 1188, 1190
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 5 cut(s) 134, 941, 1099, 1188, 1190
Ppu21I YACGTR 1 cut(s) 59
PsiI TTATAA 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 177
PspCI CACGTG 1 cut(s) 59
PspEI GGTNACC 2 cut(s) 333, 601
PspN4I GGNNCC 2 cut(s) 539, 732
PspPI GGNCC 1 cut(s) 64
PteI GCGCGC 1 cut(s) 133
PvuII CAGCTG 2 cut(s) 1082, 1159
RsaI GTAC 3 cut(s) 70, 657, 1068
RsaNI GTAC 3 cut(s) 69, 656, 1067
RseI CAYNNNNRTG 3 cut(s) 83, 623, 670
Rsr2I CGGWCCG 1 cut(s) 64
RsrII CGGWCCG 1 cut(s) 64
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 7 cut(s) 197, 255, 375, 432, 543, 677, 1020
SatI GCNGC 8 cut(s) 182, 203, 224, 360, 663, 1080, 1083, 1146
Sau3AI GATC 1 cut(s) 708
Sau96I GGNCC 1 cut(s) 64
ScaI AGTACT 1 cut(s) 657
SchI GAGTC 5 cut(s) 134, 942, 1100, 1189, 1190
SduI GDGCHC 4 cut(s) 44, 153, 177, 859
SfaNI GCATC 7 cut(s) 441, 476, 537, 764, 931, 1126, 1132
SfcI CTRYAG 1 cut(s) 211
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 3 cut(s) 83, 623, 670
SmlI CTYRAG 3 cut(s) 123, 305, 542
SmoI CTYRAG 3 cut(s) 123, 305, 542
SphI GCATGC 2 cut(s) 797, 956
Sse9I AATT 5 cut(s) 27, 555, 693, 714, 822
SsiI CCGC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 177
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 4 cut(s) 373, 418, 724, 1108
TaiI ACGT 3 cut(s) 23, 61, 1120
TaqI TCGA 1 cut(s) 960
TasI AATT 5 cut(s) 27, 555, 693, 714, 822
TatI WGTACW 2 cut(s) 655, 1066
Tru1I TTAA 7 cut(s) 197, 255, 375, 432, 543, 677, 1020
Tru9I TTAA 7 cut(s) 197, 255, 375, 432, 543, 677, 1020
TscAI CASTG 6 cut(s) 90, 195, 361, 376, 677, 1198
TseFI GTSAC 3 cut(s) 333, 352, 601
TseI GCWGC 8 cut(s) 181, 202, 223, 359, 662, 1079, 1082, 1145
Tsp45I GTSAC 3 cut(s) 333, 352, 601
TspDTI ATGAA 5 cut(s) 110, 567, 607, 656, 878
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 6 cut(s) 90, 195, 361, 376, 677, 1198
Vha464I CTTAAG 1 cut(s) 542
VneI GTGCAC 1 cut(s) 855
VpaK11BI GGWCC 1 cut(s) 64
XceI RCATGY 4 cut(s) 468, 797, 956, 1239
XmiI GTMKAC 1 cut(s) 898
ZrmI AGTACT 1 cut(s) 657
Zsp2I ATGCAT 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.