FvH4_7g22720

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002750
Physical Location & Seq
Forward (+)
17879066 .. 17882906
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g22720.t3

Sequence Viewer

Length: 2238 bp
ATGGTCATGGATCAATCATTGAGTGGATTTTATGGTTCTAGTAATGAGTTGTTGAAATTCAGGGATCAAGCTCTTTCGGACTCAATGAATAAGAAACTAGATAGTGGATTCAGGAATGGTAGTGGTTCAAATAAGGTACTCGGACATAAGTATGCTACTACAGTCCAAGTGCCTCCCACTCAAATTCCTACTAATTCTGCACTGCCATTGAGCATGATCCCAGATGGGGATTCGAACGAAGATTGTGATTTCAGTGATGTGGTTCTCAAGTACATTAGCCAGATGCTTATGGAAGAAGATATGGAAGAAAAAACTTGCATGTATCAGGAGTCTGCAGCTCTTCATTCTGCAGAGCAATCTTTTTATGAGCTTATTGGAGAGAAGCATCATCTACCTCCTGATTTGACTCCTACTGCTGATCAGAACCATGGAAGCCCAGATGAAAGCCATTCTTTACATTCCAGCTGTTGTAGTACTACCTCTACCAGTACTAGTAGTAGCATTAGGGATTGTGATTTGCTTGAGTTTAAGTATCTGAATGTTGCTTCTCAGTCAACTTCTCAGTCATCCCATGCTTCATCTAATGGAACCGGTAATTTTGTAGATGGGTTTGTGGAATCTCCTGTGAGCACTCTCAGGGTTCCTGACTTTTTTAATGAGACTGATTCAATTTTGCAATTTCAGAAAGGGTTTGAGGAAGCGAGTAAGTTTCTTCCAAATGGTAACAGCCTAATTGTGGATTTGGAGAGCAACACACCGTTGCTTAAAGAGTTGAACACAGAGGCTACAGATATGACAGCTAAGGTAGACAAGAAGAATAACCGTGAGTACCCTTCTGCTGGGTCAAGGGGAAAGAAGCATCCTTATCATGACGATGAAATTTTAGAAGGGGAGAGGAGTAACAAGCAATCATCAGTCTCTACTGAATCAGCTGTGAGTCCGGAAATGTTTGACATGGTTTTGCTCAACTGTGGACAAAGTGAATCTGCTCTTCGAGAAGCTTTGCAGAATGGAACAAAAAAGAACATACCACAGAGTGGTCAGTCAAAGGGGCCAAGTGGTGGCAAATCCCGTGGGAAGAAGCAGGGAGGTAAAAAGGATGTGGTGGATTTGAGAACCCTCTTGACAGTATGTGCACAAGCTATTGCAGCTGGTGATGAGAGAACTGGCAATGAGTTACTTAAGCGGATCAGACTGCATGCTTCTCCTGAGGGAGATGGGATGCAAAGAATGGCTTGCTATTTTGCTGATGGTCTTGAGGCACGAATGGCTGGATCTGGTACTCAGATTTACAGAGCCCTCATAGCCAAGCCAACATCTGCAGCAGATATCTTGAAGGCTTACCATCTATTTCTTGCCGCTTGTCCATTCAGGAAGCTATCAAATTTCTTTTCAAACAAGACAATAATGAATGTATCCGAGAAAGCAACAAGCCTCCACATAATTGATTTCGGTATTATGTATGGTTTTCAATGGCCTTGCCTCATTCAACGCCTCTCATCTAGGCTCGGTGGACCTCCAAAGCTCCGAATTACGGGGATTGATTTTCCAAATCCAGGTTTCCGACCAGCAGAAAGGGTTGAGGAGACAGGACGGCGCTTGGCAAACTATGCAAAAACTTTTAAAGTTCCATTTGAGTTCAATGCCATAGCACAAAAGTGGGACACCATTCAAATTGAGGATCTTAAACTCGATCGTGATGATGTGCTTGTTGTGAATAGTTGTTACAGACTTAGAAACCTACTTGATGAGACTGTGGTGGTAGACAGTCCAAGAGATATTGTCCTGAACCTGATAAGGAAGATGAATCCAGATGTCTACATTTTGGGAGTTGTAAATGGAGCCTACAGTGCACCCTTCTTTCTTTCAAGATTTCGCGAGGCTCTCTTCCACTTCTCTACAATATTTGACATGCTTGACATGTGTGTGCCCCGTGATATTCCAGAGCGAATGTTGATTGAGAGGGAGATATTTGGCCGGGAGGCAATGAATGTCATCGCATGTGAGGGTTCAGAGAGGATTGAAAGACCAGATACATACAAGCAGTGGCAGGTGAGGAATCGGAGGGCTGGTTTTACACAACTTCCTTTGAACCAGGAGATCATGGACATAGCAAAGGACAGATTGCATACATACTACCACAAGGATTTCGTAATTGATGAAGATAGCCGGTGGCTCTTGCAGGGATGGAAAGGACGCATTGTGTATGCACTTTCTTCTTGGAGGGCTGCTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

746

Amino Acids

83.43

Weight (kDa)

5.83

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 371 - 742 1.4e-119 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2041
Acc36I ACCTGC 1 cut(s) 2041
AccB7I CCANNNNNTGG 2 cut(s) 1037, 1061
AccI GTMKAC 3 cut(s) 807, 1764, 1818
AccII CGCG 1 cut(s) 1878
AccIII TCCGGA 1 cut(s) 940
AciI CCGC 2 cut(s) 1186, 1359
AclWI GGATC 6 cut(s) 18, 72, 211, 1196, 1282, 1689
AcoI YGGCCR 1 cut(s) 1975
AcsI RAATTY 4 cut(s) 56, 183, 879, 1384
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 6 cut(s) 138, 272, 475, 490, 830, 1282
AfiI CCNNNNNNNGG 7 cut(s) 226, 736, 839, 1037, 1061, 1534, 1556
AflII CTTAAG 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 1920
AgeI ACCGGT 1 cut(s) 590
AhlI ACTAGT 1 cut(s) 491
AjnI CCWGG 2 cut(s) 1555, 2094
AleI CACNNNNGTG 1 cut(s) 1657
Alw21I GWGCWC 3 cut(s) 632, 1138, 1855
Alw26I GTCTC 4 cut(s) 653, 922, 1580, 1745
Alw44I GTGCAC 2 cut(s) 1134, 1851
AlwI GGATC 6 cut(s) 18, 72, 211, 1196, 1282, 1689
Aor13HI TCCGGA 1 cut(s) 940
AoxI GGCC 3 cut(s) 1052, 1475, 1975
ApaLI GTGCAC 2 cut(s) 1134, 1851
ApeKI GCWGC 4 cut(s) 335, 1148, 1322, 2228
ApoI RAATTY 4 cut(s) 56, 183, 879, 1384
AsiGI ACCGGT 1 cut(s) 590
AspLEI GCGC 1 cut(s) 1599
AspS9I GGNCC 2 cut(s) 1052, 1514
AsuC2I CCSGG 1 cut(s) 1979
AsuHPI GGTGA 2 cut(s) 1166, 2065
AsuII TTCGAA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 1514
AxyI CCTNAGG 1 cut(s) 1209
BaeGI GKGCMC 3 cut(s) 1138, 1855, 1932
BanII GRGCYC 1 cut(s) 1300
Bbv12I GWGCWC 3 cut(s) 632, 1138, 1855
BbvI GCAGC 4 cut(s) 347, 1160, 1334, 2215
BccI CCATC 6 cut(s) 218, 599, 1211, 1244, 1353, 2181
BceAI ACGGC 1 cut(s) 1610
BciT130I CCWGG 2 cut(s) 1557, 2096
BciVI GTATCC 1 cut(s) 1426
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 1 cut(s) 1979
BcoDI GTCTC 4 cut(s) 653, 922, 1580, 1745
BcuI ACTAGT 1 cut(s) 491
BfaI CTAG 4 cut(s) 39, 98, 492, 1503
BfmI CTRYAG 6 cut(s) 159, 333, 348, 786, 1320, 1846
BfoI RGCGCY 1 cut(s) 1600
BfrI CTTAAG 1 cut(s) 1181
BfuAI ACCTGC 1 cut(s) 2041
BfuI GTATCC 1 cut(s) 1426
BisI GCNGC 5 cut(s) 336, 1149, 1323, 1359, 2229
BlsI GCNGC 5 cut(s) 337, 1150, 1324, 1360, 2230
BmcAI AGTACT 2 cut(s) 475, 490
Bme1390I CCNGG 3 cut(s) 1557, 1979, 2096
Bme18I GGWCC 1 cut(s) 1514
BmgT120I GGNCC 2 cut(s) 1052, 1514
BmiI GGNNCC 4 cut(s) 589, 642, 1053, 1843
BmrFI CCNGG 3 cut(s) 1557, 1979, 2096
BmsI GCATC 4 cut(s) 273, 394, 868, 1212
Bpu10I CCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 3 cut(s) 251, 542, 1277
BpuMI CCSGG 1 cut(s) 1979
BsaJI CCNNGG 2 cut(s) 427, 1072
BsaWI WCCGGW 2 cut(s) 590, 940
BsaXI ACNNNNNCTCC 2 cut(s) 2056, 2086
Bsc4I CCNNNNNNNGG 7 cut(s) 226, 736, 839, 1037, 1061, 1534, 1556
Bse118I RCCGGY 2 cut(s) 590, 2169
Bse1I ACTGG 2 cut(s) 486, 1171
Bse21I CCTNAGG 1 cut(s) 1209
Bse3DI GCAATG 2 cut(s) 1177, 1992
BseAI TCCGGA 1 cut(s) 940
BseBI CCWGG 2 cut(s) 1557, 2096
BseDI CCNNGG 2 cut(s) 427, 1072
BseGI GGATG 5 cut(s) 566, 859, 1105, 1227, 2192
BseLI CCNNNNNNNGG 7 cut(s) 226, 736, 839, 1037, 1061, 1534, 1556
BseMI GCAATG 2 cut(s) 1177, 1992
BseMII CTCAG 5 cut(s) 563, 575, 649, 1200, 1298
BseNI ACTGG 2 cut(s) 486, 1171
BseRI GAGGAG 2 cut(s) 910, 1598
BseSI GKGCMC 3 cut(s) 1138, 1855, 1932
BseXI GCAGC 4 cut(s) 347, 1160, 1334, 2215
BseYI CCCAGC 1 cut(s) 839
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 1878
Bsh1285I CGRYCG 1 cut(s) 1696
BshFI GGCC 3 cut(s) 1054, 1477, 1977
BshTI ACCGGT 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 1696
BsiHKAI GWGCWC 3 cut(s) 632, 1138, 1855
BsiSI CCGG 4 cut(s) 591, 941, 1978, 2170
BslFI GGGAC 1 cut(s) 1676
BslI CCNNNNNNNGG 7 cut(s) 226, 736, 839, 1037, 1061, 1534, 1556
BsmAI GTCTC 4 cut(s) 653, 922, 1580, 1745
BsmFI GGGAC 1 cut(s) 1676
BsnI GGCC 3 cut(s) 1054, 1477, 1977
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 5 cut(s) 632, 1138, 1300, 1855, 1932
Bsp13I TCCGGA 1 cut(s) 940
Bsp143I GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
Bsp19I CCATGG 1 cut(s) 427
Bsp68I TCGCGA 1 cut(s) 1878
BspACI CCGC 2 cut(s) 1186, 1359
BspANI GGCC 3 cut(s) 1054, 1477, 1977
BspCNI CTCAG 5 cut(s) 562, 574, 648, 1201, 1297
BspEI TCCGGA 1 cut(s) 940
BspFNI CGCG 1 cut(s) 1878
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 4 cut(s) 589, 642, 1053, 1843
BspMAI CTGCAG 3 cut(s) 337, 352, 1324
BspMI ACCTGC 1 cut(s) 2041
BspPI GGATC 6 cut(s) 18, 72, 211, 1196, 1282, 1689
BspQI GCTCTTC 2 cut(s) 345, 996
BspT104I TTCGAA 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 1181
BsrDI GCAATG 2 cut(s) 1177, 1992
BsrFI RCCGGY 2 cut(s) 590, 2169
BsrI ACTGG 2 cut(s) 486, 1171
BssAI RCCGGY 2 cut(s) 590, 2169
BssECI CCNNGG 2 cut(s) 427, 1072
BssMI GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 2 cut(s) 1557, 2096
Bst4CI ACNGT 8 cut(s) 163, 759, 824, 971, 1129, 1756, 1769, 1850
Bst6I CTCTTC 3 cut(s) 345, 996, 1892
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 1 cut(s) 1610
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 2 cut(s) 1200, 1237
BstDEI CTNAG 7 cut(s) 549, 561, 635, 801, 1209, 1284, 1733
BstDSI CCRYGG 2 cut(s) 427, 1072
BstF5I GGATG 5 cut(s) 566, 859, 1105, 1227, 2192
BstFNI CGCG 1 cut(s) 1878
BstH2I RGCGCY 1 cut(s) 1600
BstHHI GCGC 1 cut(s) 1599
BstKTI GATC 9 cut(s) 13, 67, 219, 421, 1191, 1277, 1684, 1696, 2103
BstMAI GTCTC 4 cut(s) 653, 922, 1580, 1745
BstMBI GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
BstMCI CGRYCG 1 cut(s) 1696
BstMWI GCNNNNNNNGC 5 cut(s) 1148, 1268, 1304, 1610, 1850
BstNI CCWGG 2 cut(s) 1557, 2096
BstNSI RCATGY 5 cut(s) 322, 1202, 1915, 1924, 2004
BstSCI CCNGG 3 cut(s) 1555, 1977, 2094
BstSFI CTRYAG 6 cut(s) 159, 333, 348, 786, 1320, 1846
BstSLI GKGCMC 3 cut(s) 1138, 1855, 1932
BstUI CGCG 1 cut(s) 1878
BstV1I GCAGC 4 cut(s) 347, 1160, 1334, 2215
BstX2I RGATCY 2 cut(s) 1274, 1681
BstYI RGATCY 2 cut(s) 1274, 1681
Bsu36I CCTNAGG 1 cut(s) 1209
BsuI GTATCC 1 cut(s) 1426
BsuRI GGCC 3 cut(s) 1054, 1477, 1977
BtgI CCRYGG 2 cut(s) 427, 1072
BtgZI GCGATG 1 cut(s) 1981
BtsCI GGATG 5 cut(s) 566, 859, 1105, 1227, 2192
BtsI GCAGTG 2 cut(s) 200, 2051
BtsIMutI CAGTG 4 cut(s) 200, 259, 1855, 2051
BtuMI TCGCGA 1 cut(s) 1878
BveI ACCTGC 1 cut(s) 2041
Cac8I GCNNGC 2 cut(s) 1200, 1237
CciI TCATGA 1 cut(s) 868
CfoI GCGC 1 cut(s) 1599
Cfr10I RCCGGY 2 cut(s) 590, 2169
Cfr13I GGNCC 2 cut(s) 1052, 1514
CseI GACGC 1 cut(s) 2205
Csp6I GTAC 6 cut(s) 137, 271, 474, 489, 829, 1281
CspAI ACCGGT 1 cut(s) 590
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviQI GTAC 6 cut(s) 137, 271, 474, 489, 829, 1281
DdeI CTNAG 7 cut(s) 549, 561, 635, 801, 1209, 1284, 1733
DpnI GATC 9 cut(s) 12, 66, 218, 420, 1190, 1276, 1683, 1695, 2102
DpnII GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
DraI TTTAAA 1 cut(s) 1624
DraIII CACNNNGTG 1 cut(s) 1037
EaeI YGGCCR 1 cut(s) 1975
Eam1104I CTCTTC 3 cut(s) 345, 996, 1892
EarI CTCTTC 3 cut(s) 345, 996, 1892
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 1300
Eco32I GATATC 1 cut(s) 1330
Eco47I GGWCC 1 cut(s) 1514
Eco81I CCTNAGG 1 cut(s) 1209
EcoRII CCWGG 2 cut(s) 1555, 2094
EcoRV GATATC 1 cut(s) 1330
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 1300
ErhI CCWWGG 1 cut(s) 427
FalI AAGNNNNNCTT 4 cut(s) 436, 468, 1219, 1251
FaqI GGGAC 1 cut(s) 1676
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 3 cut(s) 807, 1764, 1818
Fnu4HI GCNGC 5 cut(s) 336, 1149, 1323, 1359, 2229
FokI GGATG 5 cut(s) 553, 846, 1112, 1234, 2199
FriOI GRGCYC 1 cut(s) 1300
Fsp4HI GCNGC 5 cut(s) 336, 1149, 1323, 1359, 2229
FspBI CTAG 4 cut(s) 39, 98, 492, 1503
GlaI GCGC 1 cut(s) 1598
GluI GCNGC 5 cut(s) 336, 1149, 1323, 1359, 2229
GsaI CCCAGC 1 cut(s) 843
HaeII RGCGCY 1 cut(s) 1600
HaeIII GGCC 3 cut(s) 1054, 1477, 1977
HapII CCGG 4 cut(s) 591, 941, 1978, 2170
HgaI GACGC 1 cut(s) 2205
HhaI GCGC 1 cut(s) 1599
Hin6I GCGC 1 cut(s) 1597
HinP1I GCGC 1 cut(s) 1597
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HindIII AAGCTT 1 cut(s) 999
HpaII CCGG 4 cut(s) 591, 941, 1978, 2170
HphI GGTGA 2 cut(s) 1166, 2065
Hpy166II GTNNAC 8 cut(s) 555, 808, 974, 1136, 1514, 1765, 1819, 1853
Hpy8I GTNNAC 8 cut(s) 555, 808, 974, 1136, 1514, 1765, 1819, 1853
HpyAV CCTTC 4 cut(s) 843, 881, 1330, 1867
HpyCH4III ACNGT 8 cut(s) 163, 759, 824, 971, 1129, 1756, 1769, 1850
HpyF10VI GCNNNNNNNGC 5 cut(s) 1148, 1268, 1304, 1610, 1850
HpyF3I CTNAG 7 cut(s) 549, 561, 635, 801, 1209, 1284, 1733
HspAI GCGC 1 cut(s) 1597
Kpn2I TCCGGA 1 cut(s) 940
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
LguI GCTCTTC 2 cut(s) 345, 996
LmnI GCTCC 2 cut(s) 1530, 1841
Lsp1109I GCAGC 4 cut(s) 347, 1160, 1334, 2215
LweI GCATC 4 cut(s) 273, 394, 868, 1212
MaeI CTAG 4 cut(s) 39, 98, 492, 1503
MaeIII GTNAC 4 cut(s) 722, 899, 1176, 1724
MalI GATC 9 cut(s) 12, 66, 218, 420, 1190, 1276, 1683, 1695, 2102
MboI GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
MflI RGATCY 2 cut(s) 1274, 1681
MhlI GDGCHC 5 cut(s) 632, 1138, 1300, 1855, 1932
MlyI GAGTC 4 cut(s) 74, 338, 400, 946
MmeI TCCRAC 1 cut(s) 1588
MroI TCCGGA 1 cut(s) 940
MseI TTAA 6 cut(s) 528, 654, 765, 1182, 1623, 1686
MslI CAYNNNNRTG 4 cut(s) 150, 873, 1657, 1925
MspA1I CMGCKG 3 cut(s) 465, 932, 1151
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 4 cut(s) 591, 941, 1978, 2170
MspR9I CCNGG 3 cut(s) 1557, 1979, 2096
MvaI CCWGG 2 cut(s) 1557, 2096
MvnI CGCG 1 cut(s) 1878
MwoI GCNNNNNNNGC 5 cut(s) 1148, 1268, 1304, 1610, 1850
NciI CCSGG 1 cut(s) 1979
NcoI CCATGG 1 cut(s) 427
NdeII GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
NlaIV GGNNCC 4 cut(s) 589, 642, 1053, 1843
NruI TCGCGA 1 cut(s) 1878
NspI RCATGY 5 cut(s) 322, 1202, 1915, 1924, 2004
NspV TTCGAA 1 cut(s) 233
OliI CACNNNNGTG 1 cut(s) 1657
PaeI GCATGC 1 cut(s) 1202
PagI TCATGA 1 cut(s) 868
PaqCI CACCTGC 1 cut(s) 2041
PciI ACATGT 1 cut(s) 1920
PciSI GCTCTTC 2 cut(s) 345, 996
PfeI GAWTC 8 cut(s) 108, 230, 617, 665, 926, 983, 1807, 2059
PflMI CCANNNNNTGG 2 cut(s) 1037, 1061
PinAI ACCGGT 1 cut(s) 590
PkrI GCNGC 5 cut(s) 337, 1150, 1324, 1360, 2230
Ple19I CGATCG 1 cut(s) 1696
PleI GAGTC 4 cut(s) 74, 337, 400, 945
PpsI GAGTC 4 cut(s) 74, 337, 400, 945
PscI ACATGT 1 cut(s) 1920
Psp6I CCWGG 2 cut(s) 1555, 2094
PspFI CCCAGC 1 cut(s) 839
PspGI CCWGG 2 cut(s) 1555, 2094
PspN4I GGNNCC 4 cut(s) 589, 642, 1053, 1843
PspPI GGNCC 2 cut(s) 1052, 1514
PstI CTGCAG 3 cut(s) 337, 352, 1324
PsuI RGATCY 2 cut(s) 1274, 1681
PvuI CGATCG 1 cut(s) 1696
PvuII CAGCTG 3 cut(s) 465, 932, 1151
RruI TCGCGA 1 cut(s) 1878
RsaI GTAC 6 cut(s) 138, 272, 475, 490, 830, 1282
RsaNI GTAC 6 cut(s) 137, 271, 474, 489, 829, 1281
RseI CAYNNNNRTG 4 cut(s) 150, 873, 1657, 1925
SapI GCTCTTC 2 cut(s) 345, 996
SaqAI TTAA 6 cut(s) 528, 654, 765, 1182, 1623, 1686
SatI GCNGC 5 cut(s) 336, 1149, 1323, 1359, 2229
Sau3AI GATC 9 cut(s) 10, 64, 216, 418, 1188, 1274, 1681, 1693, 2100
Sau96I GGNCC 2 cut(s) 1052, 1514
ScaI AGTACT 2 cut(s) 475, 490
SchI GAGTC 4 cut(s) 74, 338, 400, 946
ScrFI CCNGG 3 cut(s) 1557, 1979, 2096
SduI GDGCHC 5 cut(s) 632, 1138, 1300, 1855, 1932
SfaNI GCATC 4 cut(s) 273, 394, 868, 1212
SfcI CTRYAG 6 cut(s) 159, 333, 348, 786, 1320, 1846
SfuI TTCGAA 1 cut(s) 233
SinI GGWCC 1 cut(s) 1514
SmiMI CAYNNNNRTG 4 cut(s) 150, 873, 1657, 1925
SmlI CTYRAG 4 cut(s) 266, 521, 1181, 1256
SmoI CTYRAG 4 cut(s) 266, 521, 1181, 1256
SpeI ACTAGT 1 cut(s) 491
SphI GCATGC 1 cut(s) 1202
SsiI CCGC 2 cut(s) 1186, 1359
SspI AATATT 1 cut(s) 1905
SspMI CTAG 4 cut(s) 39, 98, 492, 1503
StyD4I CCNGG 3 cut(s) 1555, 1977, 2094
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 8 cut(s) 163, 759, 824, 971, 1129, 1756, 1769, 1850
TaqI TCGA 3 cut(s) 233, 994, 1692
TatI WGTACW 3 cut(s) 270, 473, 488
TauI GCSGC 1 cut(s) 1361
TfiI GAWTC 8 cut(s) 108, 230, 617, 665, 926, 983, 1807, 2059
Tru1I TTAA 6 cut(s) 528, 654, 765, 1182, 1623, 1686
Tru9I TTAA 6 cut(s) 528, 654, 765, 1182, 1623, 1686
TscAI CASTG 4 cut(s) 207, 259, 1855, 2051
TseI GCWGC 4 cut(s) 335, 1148, 1322, 2228
TspDTI ATGAA 9 cut(s) 101, 332, 456, 567, 891, 1424, 1820, 2003, 2175
TspRI CASTG 4 cut(s) 207, 259, 1855, 2051
Van91I CCANNNNNTGG 2 cut(s) 1037, 1061
Vha464I CTTAAG 1 cut(s) 1181
VneI GTGCAC 2 cut(s) 1134, 1851
VpaK11BI GGWCC 1 cut(s) 1514
XapI RAATTY 4 cut(s) 56, 183, 879, 1384
XceI RCATGY 5 cut(s) 322, 1202, 1915, 1924, 2004
XmiI GTMKAC 3 cut(s) 807, 1764, 1818
XspI CTAG 4 cut(s) 39, 98, 492, 1503
ZrmI AGTACT 2 cut(s) 475, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.