Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002750

Physical Location & Seq

Genomic Coordinates Forward (+)

22266875 .. 22270230

Gene Structure 3356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1143500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGGCAATGGATCCTCGTCTTAGGGCTTTCTCTGATTCTTTCAATGGAGTTCAATTGGGGAACCAACCTTTACCCATTCTTTCACAACCAACCGTTGTAGCCGGATCCCGATTCGATAGTAAAGCCTGCTTGGACAATAATTACAGAAATTTCGATTACCCTCAACCCGGTCTTACCCCGAATAGTGTATCTTCGTTCTCGAGTGTGAGTCCTGAGGATGATTCTGCCGAAGATTGTGACTTTTCGGATGTAGTCTTGAAGTACATAAACCAGATGCTCATGGAAGAAGATATGGAGGAAAAAACCTGCATGCTTCAAGAGTCGTTGGAGCTTCAAGCGGCCGAGAAGTCATTCTATGAGGTCCTTGGAAAGAAATATCCCCCCTCCCCTGATTTATACCGGGGTTATGCCAGTCAATATGGTGAGAGCCCGGGTGAAAGTTCATCTGGAACTTGGAGTAACTATATCACTAGTAGTTGCAACAGTGGTGGTTATTTCGGTGATAATTCCTCGATTCAAAGTCCAGATGGATATTCGTCTCAGTTACAGGGTCTTCAGGATTATAGCATTTCTCAATCATTTAGCGGGTCTTCAACTAGGGTCAGTAGCCTCGACGGGCTGGTGGATGCTCCTAGTAGTAGTATTTATACCCCAGATTTGAAGACTGAGAGCCAATCTATTTGGCAGTTCAGGAAGGGGGTTGAGGAGGCTAGTAGATTTCTCCCTTCCGAGACTAATTTGGTTGTTGATTTGGAGGCAAATGGGATGTCGGTACGCGCGCCAAATGTTGGGACAGGTGCGCCATTTGTCAAGGCGGAGAGAAAGGATGGAGGAGAATATTCTTCTGGTAGGTCAAGGGGAAAGAAGAATCTATATAGGGACGACGAAGATGTAGAGGAAAAAAGGAGTAGCAAGCAAGCTGCTGTTTCGTCTGAATCTCCTCTGCGGTCAGAGATGTTTGATGTTGTATTGCTTTGTAGCACAGGGGAGGGTCGGGAGCGTTTGGGTTCTCTTCGTGAGGCCTTGCAAAATGGAATGAGCAAAAGTGTGCCACAGAACAGGCAATCCACAGGATCTAATCGAGGAAAAGGTCGTGGTAAGAAACTGAGTGTTAAAAAGGAGGTGGTGGATTTGAGAACCCTCCTAATTAGTTGTGCACAAGCAGCTGCAGCTGATGATCACAGGACCGCAAATGAACTGCTAAGGAAGGTCAGACAGCATTCCTCACCTTTTGGAGACGGGACACAGAGACTTGCTCATTGCTTTGCTAATGGCCTTGAGGCACGCTTGGCTGGTACTGGTAGCCAGATTTATAAAGGTCTTGTTAGCAAAAGGACATCGGCTGCCGATATCTTGAAAGCTTATCATCTGTACCTTGCTGCATCTCCATTTAAGAAGATGTCTAATTTTGTTTCAAACGTCACAATCAGGAATTTAGCAGAAAAATCGACAAGGCTCCACGTCATTGATTTTGGTATCCTTTATGGTTTCCAGTGGCCCACCCTTATTCAACGGATCTCATGGAGGGAAGGTGGACCCCCAAAGGTTCGGATTACTGGAATTGAATTTCCTCAACCAGGATTTCGGCCAGCTGAGCGAGTTGAGGAAACAGGAAGACGTTTGGCAGCTTATGCTGAGAACTTCAAAGTGCCGTTTGAGTACAATGCTATTGCAAAGAAATGGGAAACCATTACACTTGAGGAACTCAAGATTGACAAGGATGAAGTTCTTGTTGTGAACTTTATATATCGGGGTAAGAACTTGCTTGATGAAAGTGTGTCTGTGGATAGCGTAAGAAACAGAGTTCTTGCTTTGATAAGGAGAATCAATCCAGACATTTTTATCCATGGAATTGTTAACGGAGCGTACAATGCCCCCTTCTTTGTTACCAGATTTCGAGAGGCGTTATTTCATTTTTCTGCACTGTTTGATATGCTTGAAACTGTTGTTCCACGCGAGGATCAGGAGAGGATGTTAATTGAGAAAGAGATCTTTGGTAGGGAGGCTATGAATGTTATAGCCTGTGAGGGCTGGGAGAGAGTCGAAAGGCCAGAAACGTACAAGCAATGGCAAGTTCGTAACTTGAGGGCTGGATTTGAGCAAATACCTTTTGACCGAGAGCTTGTAAAGCGGGCGGCTAAGAAAGTGAGCTCTCTTTACCACAAGGACTTTGTCATTGATGAAAATAGCCAGTGGCTGTTGCAGGGATGGAAGGGACGAACCGTCTTTGCCCTCTCTGCTTGGAAACCTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRAS

Protein Analysis

752

Amino Acids

84.13

Weight (kDa)

6.77

Isoelectric Point (pI)

49.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRAS	PF03514	378 - 749	2.3e-122	GRAS domain family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37650 AT2G37650
fragaria_vesca	FvH4_6g13520 FvH4_6g13520 FvH4_6g13520 FvH4_7g22720 FvH4_7g22720 FvH4_7g22720 FvH4_7g22720
malus_domestica	MD04G1130400.v1.1 MD12G1143500.v1.1
prunus_persica	Prupe.2G237300_v2.0.a1 Prupe.6G257000_v2.0.a1 Prupe.6G257000_v2.0.a1 Prupe.6G257000_v2.0.a1 Prupe.6G257000_v2.0.a1
pyrus_communis	pycom04g11860 pycom12g13820
rosa_chinensis	RchiOBHm_Chr1g0367811 RchiOBHm_Chr3g0466391
rosa_laevigata	RLG00000024578 RLG00000027261
rosa_multiflora	Rmu_sc0001791.1_g000005 Rmu_sc0003480.1_g000003 Rmu_sc0010008.1_g000006
rosa_roxburghii	Rroxscaffold_4G00289190 Rroxscaffold_6G00414510
rosa_rugosa	Rorug01G0336900 Rorug01G0337000 Rorug03G0081400
rosa_samantha	Rh1AG344000 Rh1BG306000 Rh1CG321500 Rh1DG338200 Rh3AG128000 Rh3BG149300 Rh3DG150000
rosa_wichuraiana	Rw1G030620 Rw3G012090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1314
Acc36I	ACCTGC	1 cut(s)	314
AccB7I	CCANNNNNTGG	1 cut(s)	788
AccII	CGCG	3 cut(s)	777, 779, 1956
AciI	CCGC	7 cut(s)	338, 585, 815, 946, 1188, 2131, 2135
AclWI	GGATC	7 cut(s)	5, 18, 99, 112, 1081, 1523, 1968
AcoI	YGGCCR	2 cut(s)	339, 1586
AcsI	RAATTY	3 cut(s)	148, 1432, 1565
AcuI	CTGAAG	1 cut(s)	539
AfaI	GTAC	7 cut(s)	263, 774, 1297, 1373, 1661, 1868, 2060
AfiI	CCNNNNNNNGG	3 cut(s)	167, 788, 1577
AhdI	GACNNNNNGTC	1 cut(s)	2222
AhlI	ACTAGT	1 cut(s)	470
AjiI	CACGTC	1 cut(s)	1462
AjnI	CCWGG	1 cut(s)	1576
AloI	GAACNNNNNNTCC	2 cut(s)	1049, 1081
AluBI	AGCT	9 cut(s)	331, 920, 1166, 1172, 1361, 1592, 1628, 2122, 2151
AluI	AGCT	9 cut(s)	331, 920, 1166, 1172, 1361, 1592, 1628, 2122, 2151
Alw21I	GWGCWC	2 cut(s)	1159, 2153
Alw26I	GTCTC	4 cut(s)	543, 725, 1230, 1243
Alw44I	GTGCAC	1 cut(s)	1155
AlwI	GGATC	7 cut(s)	5, 18, 99, 112, 1081, 1523, 1968
AlwNI	CAGNNNCTG	1 cut(s)	2197
Ama87I	CYCGRG	2 cut(s)	199, 430
AoxI	GGCC	6 cut(s)	339, 1020, 1273, 1496, 1586, 2048
ApaLI	GTGCAC	1 cut(s)	1155
ApeKI	GCWGC	7 cut(s)	920, 1163, 1166, 1169, 1343, 1379, 1625
ApoI	RAATTY	3 cut(s)	148, 1432, 1565
ArsI	GACNNNNNNTTYG	2 cut(s)	752, 784
Asp700I	GAANNNNTTC	1 cut(s)	350
AspLEI	GCGC	3 cut(s)	779, 781, 802
AspS9I	GGNCC	4 cut(s)	361, 1185, 1497, 1535
AsuC2I	CCSGG	4 cut(s)	168, 401, 431, 432
AsuHPI	GGTGA	4 cut(s)	434, 446, 512, 1218
AvaI	CYCGRG	2 cut(s)	199, 430
AvaII	GGWCC	3 cut(s)	361, 1185, 1535
AxyI	CCTNAGG	1 cut(s)	213
BaeGI	GKGCMC	1 cut(s)	1159
BamHI	GGATCC	2 cut(s)	10, 104
BanII	GRGCYC	2 cut(s)	431, 2153
BbsI	GAAGAC	4 cut(s)	545, 582, 668, 1621
Bbv12I	GWGCWC	2 cut(s)	1159, 2153
BbvI	GCAGC	7 cut(s)	907, 1153, 1175, 1181, 1330, 1366, 1637
BccI	CCATC	3 cut(s)	521, 821, 2202
BceAI	ACGGC	1 cut(s)	1636
BcgI	CGANNNNNNTGC	2 cut(s)	1428, 1462
BciT130I	CCWGG	1 cut(s)	1578
BciVI	GTATCC	1 cut(s)	1487
BclI	TGATCA	1 cut(s)	1177
BcnI	CCSGG	4 cut(s)	168, 401, 431, 432
BcoDI	GTCTC	4 cut(s)	543, 725, 1230, 1243
BcuI	ACTAGT	1 cut(s)	470
BfaI	CTAG	4 cut(s)	471, 597, 633, 711
BfmI	CTRYAG	1 cut(s)	1167
BfuAI	ACCTGC	1 cut(s)	314
BfuI	GTATCC	1 cut(s)	1487
BglII	AGATCT	1 cut(s)	1989
BisI	GCNGC	9 cut(s)	339, 921, 1164, 1167, 1170, 1344, 1380, 1626, 2136
BlpI	GCTNAGC	1 cut(s)	1593
BlsI	GCNGC	9 cut(s)	340, 922, 1165, 1168, 1171, 1345, 1381, 1627, 2137
Bme1390I	CCNGG	5 cut(s)	168, 401, 431, 432, 1578
Bme18I	GGWCC	3 cut(s)	361, 1185, 1535
BmeRI	GACNNNNNGTC	1 cut(s)	2222
BmeT110I	CYCGRG	2 cut(s)	199, 430
BmgBI	CACGTC	1 cut(s)	1462
BmgT120I	GGNCC	4 cut(s)	361, 1185, 1497, 1535
BmiI	GGNNCC	5 cut(s)	12, 62, 106, 1457, 1537
BmrFI	CCNGG	5 cut(s)	168, 401, 431, 432, 1578
BmsI	GCATC	3 cut(s)	264, 616, 1391
BpiI	GAAGAC	4 cut(s)	545, 582, 668, 1621
BplI	GAGNNNNNCTC	2 cut(s)	1240, 1272
Bpu10I	CCTNAGC	1 cut(s)	1202
Bpu1102I	GCTNAGC	1 cut(s)	1593
BpuEI	CTTGAG	4 cut(s)	1298, 1691, 1718, 2104
BpuMI	CCSGG	4 cut(s)	168, 401, 431, 432
BsaJI	CCNNGG	4 cut(s)	364, 400, 430, 1846
BsaXI	ACNNNNNCTCC	4 cut(s)	1516, 1546, 1958, 1988
Bsc4I	CCNNNNNNNGG	3 cut(s)	167, 788, 1577
Bse1I	ACTGG	5 cut(s)	411, 1303, 1492, 1561, 2191
Bse21I	CCTNAGG	1 cut(s)	213
Bse3DI	GCAATG	3 cut(s)	12, 1258, 2072
BseBI	CCWGG	1 cut(s)	1578
BseDI	CCNNGG	4 cut(s)	364, 400, 430, 1846
BseGI	GGATG	8 cut(s)	223, 253, 631, 771, 832, 1726, 1977, 2213
BseLI	CCNNNNNNNGG	3 cut(s)	167, 788, 1577
BseMI	GCAATG	3 cut(s)	12, 1258, 2072
BseMII	CTCAG	6 cut(s)	204, 554, 657, 1097, 1584, 1626
BseNI	ACTGG	5 cut(s)	411, 1303, 1492, 1561, 2191
BsePI	GCGCGC	1 cut(s)	777
BseRI	GAGGAG	3 cut(s)	719, 846, 930
BseSI	GKGCMC	1 cut(s)	1159
BseX3I	CGGCCG	1 cut(s)	339
BseXI	GCAGC	7 cut(s)	907, 1153, 1175, 1181, 1330, 1366, 1637
BseYI	CCCAGC	1 cut(s)	2031
BsgI	GTGCAG	1 cut(s)	1905
Bsh1236I	CGCG	3 cut(s)	777, 779, 1956
Bsh1285I	CGRYCG	1 cut(s)	342
BshFI	GGCC	6 cut(s)	341, 1022, 1275, 1498, 1588, 2050
BsiEI	CGRYCG	1 cut(s)	342
BsiHKAI	GWGCWC	2 cut(s)	1159, 2153
BsiHKCI	CYCGRG	2 cut(s)	199, 430
BsiSI	CCGG	4 cut(s)	102, 168, 400, 431
BslFI	GGGAC	4 cut(s)	805, 893, 1255, 2229
BslI	CCNNNNNNNGG	3 cut(s)	167, 788, 1577
BsmAI	GTCTC	4 cut(s)	543, 725, 1230, 1243
BsmBI	CGTCTC	2 cut(s)	543, 1230
BsmFI	GGGAC	4 cut(s)	805, 893, 1255, 2229
BsmI	GAATGC	1 cut(s)	1219
BsnI	GGCC	6 cut(s)	341, 1022, 1275, 1498, 1588, 2050
BsoBI	CYCGRG	2 cut(s)	199, 430
Bsp1286I	GDGCHC	3 cut(s)	431, 1159, 2153
Bsp143I	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
Bsp1720I	GCTNAGC	1 cut(s)	1593
Bsp19I	CCATGG	1 cut(s)	1846
BspACI	CCGC	7 cut(s)	338, 585, 815, 946, 1188, 2131, 2135
BspANI	GGCC	6 cut(s)	341, 1022, 1275, 1498, 1588, 2050
BspCNI	CTCAG	6 cut(s)	205, 553, 658, 1098, 1585, 1627
BspFNI	CGCG	3 cut(s)	777, 779, 1956
BspLI	GGNNCC	5 cut(s)	12, 62, 106, 1457, 1537
BspMAI	CTGCAG	1 cut(s)	1171
BspMI	ACCTGC	1 cut(s)	314
BspPI	GGATC	7 cut(s)	5, 18, 99, 112, 1081, 1523, 1968
BsrDI	GCAATG	3 cut(s)	12, 1258, 2072
BsrI	ACTGG	5 cut(s)	411, 1303, 1492, 1561, 2191
BssECI	CCNNGG	4 cut(s)	364, 400, 430, 1846
BssHII	GCGCGC	1 cut(s)	777
BssMI	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
BssT1I	CCWWGG	2 cut(s)	364, 1846
Bst2UI	CCWGG	1 cut(s)	1578
Bst4CI	ACNGT	5 cut(s)	94, 485, 1926, 1945, 2224
Bst6I	CTCTTC	1 cut(s)	1017
BstAPI	GCANNNNNTGC	1 cut(s)	1631
BstC8I	GCNNGC	8 cut(s)	127, 311, 779, 914, 918, 1285, 1590, 2133
BstDEI	CTNAG	9 cut(s)	20, 213, 540, 666, 1106, 1202, 1593, 1635, 2139
BstDSI	CCRYGG	1 cut(s)	1846
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	8 cut(s)	223, 253, 631, 771, 832, 1726, 1977, 2213
BstFNI	CGCG	3 cut(s)	777, 779, 1956
BstHHI	GCGC	3 cut(s)	779, 781, 802
BstKTI	GATC	7 cut(s)	13, 107, 1076, 1180, 1518, 1963, 1992
BstMAI	GTCTC	4 cut(s)	543, 725, 1230, 1243
BstMBI	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
BstMCI	CGRYCG	1 cut(s)	342
BstMWI	GCNNNNNNNGC	8 cut(s)	1163, 1169, 1289, 1594, 1631, 1871, 2128, 2237
BstNI	CCWGG	1 cut(s)	1578
BstNSI	RCATGY	1 cut(s)	313
BstSCI	CCNGG	5 cut(s)	166, 399, 429, 430, 1576
BstSFI	CTRYAG	1 cut(s)	1167
BstSLI	GKGCMC	1 cut(s)	1159
BstUI	CGCG	3 cut(s)	777, 779, 1956
BstV1I	GCAGC	7 cut(s)	907, 1153, 1175, 1181, 1330, 1366, 1637
BstV2I	GAAGAC	4 cut(s)	545, 582, 668, 1621
BstX2I	RGATCY	5 cut(s)	10, 104, 1073, 1515, 1989
BstYI	RGATCY	5 cut(s)	10, 104, 1073, 1515, 1989
BstZI	CGGCCG	1 cut(s)	339
Bsu36I	CCTNAGG	1 cut(s)	213
BsuI	GTATCC	1 cut(s)	1487
BsuRI	GGCC	6 cut(s)	341, 1022, 1275, 1498, 1588, 2050
BtgI	CCRYGG	1 cut(s)	1846
BtrI	CACGTC	1 cut(s)	1462
BtsCI	GGATG	8 cut(s)	223, 253, 631, 771, 832, 1726, 1977, 2213
BtsIMutI	CAGTG	4 cut(s)	490, 1499, 1922, 2198
BveI	ACCTGC	1 cut(s)	314
Cac8I	GCNNGC	8 cut(s)	127, 311, 779, 914, 918, 1285, 1590, 2133
CaiI	CAGNNNCTG	1 cut(s)	2197
CfoI	GCGC	3 cut(s)	779, 781, 802
Cfr13I	GGNCC	4 cut(s)	361, 1185, 1497, 1535
Cfr9I	CCCGGG	1 cut(s)	430
Csp6I	GTAC	7 cut(s)	262, 773, 1296, 1372, 1660, 1867, 2059
CspCI	CAANNNNNGTGG	2 cut(s)	469, 504
CviAII	CATG	4 cut(s)	280, 310, 1521, 1847
CviQI	GTAC	7 cut(s)	262, 773, 1296, 1372, 1660, 1867, 2059
DdeI	CTNAG	9 cut(s)	20, 213, 540, 666, 1106, 1202, 1593, 1635, 2139
DpnI	GATC	7 cut(s)	12, 106, 1075, 1179, 1517, 1962, 1991
DpnII	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
DriI	GACNNNNNGTC	1 cut(s)	2222
EaeI	YGGCCR	2 cut(s)	339, 1586
EagI	CGGCCG	1 cut(s)	339
Eam1104I	CTCTTC	1 cut(s)	1017
Eam1105I	GACNNNNNGTC	1 cut(s)	2222
EarI	CTCTTC	1 cut(s)	1017
EciI	GGCGGA	1 cut(s)	830
Ecl136II	GAGCTC	1 cut(s)	2151
EclXI	CGGCCG	1 cut(s)	339
Eco130I	CCWWGG	2 cut(s)	364, 1846
Eco147I	AGGCCT	1 cut(s)	1022
Eco24I	GRGCYC	2 cut(s)	431, 2153
Eco32I	GATATC	1 cut(s)	1351
Eco47I	GGWCC	3 cut(s)	361, 1185, 1535
Eco52I	CGGCCG	1 cut(s)	339
Eco53kI	GAGCTC	1 cut(s)	2151
Eco57I	CTGAAG	1 cut(s)	539
Eco81I	CCTNAGG	1 cut(s)	213
Eco88I	CYCGRG	2 cut(s)	199, 430
EcoICRI	GAGCTC	1 cut(s)	2151
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoO109I	RGGNCCY	1 cut(s)	361
EcoRII	CCWGG	1 cut(s)	1576
EcoRV	GATATC	1 cut(s)	1351
EcoT14I	CCWWGG	2 cut(s)	364, 1846
EcoT38I	GRGCYC	2 cut(s)	431, 2153
ErhI	CCWWGG	2 cut(s)	364, 1846
Esp3I	CGTCTC	2 cut(s)	543, 1230
FaeI	CATG	4 cut(s)	283, 313, 1524, 1850
FaqI	GGGAC	4 cut(s)	805, 893, 1255, 2229
FatI	CATG	4 cut(s)	279, 309, 1520, 1846
FauI	CCCGC	2 cut(s)	578, 2124
FbaI	TGATCA	1 cut(s)	1177
Fnu4HI	GCNGC	9 cut(s)	339, 921, 1164, 1167, 1170, 1344, 1380, 1626, 2136
FokI	GGATG	8 cut(s)	230, 260, 638, 778, 839, 1733, 1984, 2220
FriOI	GRGCYC	2 cut(s)	431, 2153
Fsp4HI	GCNGC	9 cut(s)	339, 921, 1164, 1167, 1170, 1344, 1380, 1626, 2136
FspBI	CTAG	4 cut(s)	471, 597, 633, 711
GlaI	GCGC	3 cut(s)	778, 780, 801
GluI	GCNGC	9 cut(s)	339, 921, 1164, 1167, 1170, 1344, 1380, 1626, 2136
GsaI	CCCAGC	1 cut(s)	2035
HaeIII	GGCC	6 cut(s)	341, 1022, 1275, 1498, 1588, 2050
HapII	CCGG	4 cut(s)	102, 168, 400, 431
HhaI	GCGC	3 cut(s)	779, 781, 802
Hin1II	CATG	4 cut(s)	283, 313, 1524, 1850
Hin6I	GCGC	3 cut(s)	777, 779, 800
HinP1I	GCGC	3 cut(s)	777, 779, 800
HincII	GTYRAC	1 cut(s)	1858
HindII	GTYRAC	1 cut(s)	1858
HindIII	AAGCTT	1 cut(s)	1359
HpaI	GTTAAC	1 cut(s)	1858
HpaII	CCGG	4 cut(s)	102, 168, 400, 431
HphI	GGTGA	4 cut(s)	434, 446, 512, 1218
Hpy166II	GTNNAC	4 cut(s)	1157, 1535, 1738, 1858
Hpy188I	TCNGA	7 cut(s)	34, 247, 730, 934, 952, 1214, 1551
Hpy8I	GTNNAC	4 cut(s)	1157, 1535, 1738, 1858
Hpy99I	CGWCG	2 cut(s)	617, 887
HpyAV	CCTTC	6 cut(s)	688, 735, 1201, 1523, 1888, 2206
HpyCH4III	ACNGT	5 cut(s)	94, 485, 1926, 1945, 2224
HpyCH4IV	ACGT	4 cut(s)	1419, 1461, 1618, 2057
HpyCH4V	TGCA	9 cut(s)	309, 480, 1027, 1157, 1169, 1382, 1673, 1922, 2203
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1163, 1169, 1289, 1594, 1631, 1871, 2128, 2237
HpyF3I	CTNAG	9 cut(s)	20, 213, 540, 666, 1106, 1202, 1593, 1635, 2139
HpySE526I	ACGT	4 cut(s)	1419, 1461, 1618, 2057
Hsp92II	CATG	4 cut(s)	283, 313, 1524, 1850
HspAI	GCGC	3 cut(s)	777, 779, 800
Ksp22I	TGATCA	1 cut(s)	1177
KspAI	GTTAAC	1 cut(s)	1858
Kzo9I	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
LmnI	GCTCC	5 cut(s)	328, 634, 997, 1461, 1862
Lsp1109I	GCAGC	7 cut(s)	907, 1153, 1175, 1181, 1330, 1366, 1637
LweI	GCATC	3 cut(s)	264, 616, 1391
MaeI	CTAG	4 cut(s)	471, 597, 633, 711
MaeII	ACGT	4 cut(s)	1419, 1461, 1618, 2057
MaeIII	GTNAC	6 cut(s)	236, 458, 543, 1420, 1885, 2078
MalI	GATC	7 cut(s)	12, 106, 1075, 1179, 1517, 1962, 1991
MboI	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
MfeI	CAATTG	1 cut(s)	53
MflI	RGATCY	5 cut(s)	10, 104, 1073, 1515, 1989
MhlI	GDGCHC	3 cut(s)	431, 1159, 2153
MlyI	GAGTC	3 cut(s)	217, 329, 2049
MmeI	TCCRAC	1 cut(s)	306
MroXI	GAANNNNTTC	1 cut(s)	350
MseI	TTAA	5 cut(s)	1113, 1392, 1857, 1976, 2254
MspA1I	CMGCKG	3 cut(s)	1166, 1172, 1592
MspI	CCGG	4 cut(s)	102, 168, 400, 431
MspR9I	CCNGG	5 cut(s)	168, 401, 431, 432, 1578
MunI	CAATTG	1 cut(s)	53
Mva1269I	GAATGC	1 cut(s)	1219
MvaI	CCWGG	1 cut(s)	1578
MvnI	CGCG	3 cut(s)	777, 779, 1956
MwoI	GCNNNNNNNGC	8 cut(s)	1163, 1169, 1289, 1594, 1631, 1871, 2128, 2237
NciI	CCSGG	4 cut(s)	168, 401, 431, 432
NcoI	CCATGG	1 cut(s)	1846
NdeII	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
NlaIII	CATG	4 cut(s)	283, 313, 1524, 1850
NlaIV	GGNNCC	5 cut(s)	12, 62, 106, 1457, 1537
NmeAIII	GCCGAG	1 cut(s)	367
NmuCI	GTSAC	2 cut(s)	236, 1420
NspI	RCATGY	1 cut(s)	313
PaeI	GCATGC	1 cut(s)	313
PaeR7I	CTCGAG	1 cut(s)	199
PauI	GCGCGC	1 cut(s)	777
PceI	AGGCCT	1 cut(s)	1022
PctI	GAATGC	1 cut(s)	1219
PdmI	GAANNNNTTC	1 cut(s)	350
PfeI	GAWTC	7 cut(s)	35, 111, 221, 514, 868, 935, 1824
PflFI	GACNNNGTC	1 cut(s)	2171
PflMI	CCANNNNNTGG	1 cut(s)	788
PkrI	GCNGC	9 cut(s)	340, 922, 1165, 1168, 1171, 1345, 1381, 1627, 2137
PleI	GAGTC	3 cut(s)	216, 328, 2048
PpsI	GAGTC	3 cut(s)	216, 328, 2048
PpuMI	RGGWCCY	1 cut(s)	361
PsiI	TTATAA	1 cut(s)	1314
Psp124BI	GAGCTC	1 cut(s)	2153
Psp5II	RGGWCCY	1 cut(s)	361
Psp6I	CCWGG	1 cut(s)	1576
PspFI	CCCAGC	1 cut(s)	2031
PspGI	CCWGG	1 cut(s)	1576
PspN4I	GGNNCC	5 cut(s)	12, 62, 106, 1457, 1537
PspPI	GGNCC	4 cut(s)	361, 1185, 1497, 1535
PspPPI	RGGWCCY	1 cut(s)	361
PstI	CTGCAG	1 cut(s)	1171
PstNI	CAGNNNCTG	1 cut(s)	2197
PsuI	RGATCY	5 cut(s)	10, 104, 1073, 1515, 1989
PsyI	GACNNNGTC	1 cut(s)	2171
PteI	GCGCGC	1 cut(s)	777
PvuII	CAGCTG	3 cut(s)	1166, 1172, 1592
RsaI	GTAC	7 cut(s)	263, 774, 1297, 1373, 1661, 1868, 2060
RsaNI	GTAC	7 cut(s)	262, 773, 1296, 1372, 1660, 1867, 2059
SacI	GAGCTC	1 cut(s)	2153
SaqAI	TTAA	5 cut(s)	1113, 1392, 1857, 1976, 2254
SatI	GCNGC	9 cut(s)	339, 921, 1164, 1167, 1170, 1344, 1380, 1626, 2136
Sau3AI	GATC	7 cut(s)	10, 104, 1073, 1177, 1515, 1960, 1989
Sau96I	GGNCC	4 cut(s)	361, 1185, 1497, 1535
SchI	GAGTC	3 cut(s)	217, 329, 2049
ScrFI	CCNGG	5 cut(s)	168, 401, 431, 432, 1578
SduI	GDGCHC	3 cut(s)	431, 1159, 2153
SfaNI	GCATC	3 cut(s)	264, 616, 1391
SfcI	CTRYAG	1 cut(s)	1167
Sfr274I	CTCGAG	1 cut(s)	199
SinI	GGWCC	3 cut(s)	361, 1185, 1535
SlaI	CTCGAG	1 cut(s)	199
SmaI	CCCGGG	1 cut(s)	432
SmlI	CTYRAG	5 cut(s)	199, 1277, 1697, 1706, 2083
SmoI	CTYRAG	5 cut(s)	199, 1277, 1697, 1706, 2083
SpeI	ACTAGT	1 cut(s)	470
SphI	GCATGC	1 cut(s)	313
SseBI	AGGCCT	1 cut(s)	1022
SsiI	CCGC	7 cut(s)	338, 585, 815, 946, 1188, 2131, 2135
SspI	AATATT	1 cut(s)	839
SspMI	CTAG	4 cut(s)	471, 597, 633, 711
SstI	GAGCTC	1 cut(s)	2153
StuI	AGGCCT	1 cut(s)	1022
StyD4I	CCNGG	5 cut(s)	166, 399, 429, 430, 1576
StyI	CCWWGG	2 cut(s)	364, 1846
TaaI	ACNGT	5 cut(s)	94, 485, 1926, 1945, 2224
TaiI	ACGT	4 cut(s)	1422, 1464, 1621, 2060
TaqI	TCGA	9 cut(s)	114, 153, 200, 512, 612, 1081, 1448, 1897, 2043
TaqII	GACCGA	1 cut(s)	2130
TatI	WGTACW	2 cut(s)	261, 1659
TauI	GCSGC	2 cut(s)	341, 2138
TfiI	GAWTC	7 cut(s)	35, 111, 221, 514, 868, 935, 1824
Tru1I	TTAA	5 cut(s)	1113, 1392, 1857, 1976, 2254
Tru9I	TTAA	5 cut(s)	1113, 1392, 1857, 1976, 2254
TscAI	CASTG	4 cut(s)	490, 1499, 1929, 2198
TseFI	GTSAC	2 cut(s)	236, 1420
TseI	GCWGC	7 cut(s)	920, 1163, 1166, 1169, 1343, 1379, 1625
Tsp45I	GTSAC	2 cut(s)	236, 1420
TspDTI	ATGAA	7 cut(s)	432, 1209, 1737, 1785, 1901, 2024, 2196
TspGWI	ACGGA	2 cut(s)	1528, 1875
TspMI	CCCGGG	1 cut(s)	430
TspRI	CASTG	4 cut(s)	490, 1499, 1929, 2198
Tth111I	GACNNNGTC	1 cut(s)	2171
Van91I	CCANNNNNTGG	1 cut(s)	788
VneI	GTGCAC	1 cut(s)	1155
VpaK11BI	GGWCC	3 cut(s)	361, 1185, 1535
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	3 cut(s)	148, 1432, 1565
XceI	RCATGY	1 cut(s)	313
XhoI	CTCGAG	1 cut(s)	199
XmaI	CCCGGG	1 cut(s)	430
XmnI	GAANNNNTTC	1 cut(s)	350
XspI	CTAG	4 cut(s)	471, 597, 633, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.