FvH4_7g23150

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004604
Physical Location & Seq
Forward (+)
18119417 .. 18124697
5281 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23150.t2

Sequence Viewer

Length: 1419 bp
ATGAATGACGGCGGAGATGTGAGAATGGAGGAGGGAGACGACGGCGGGGAGAAGGTGGTGTTCATGTGGGGATATCTTCCGGGAGCTCTGCCGCAAAGGTCGCCGCTTTTGTCGCCGACGGTTGTGAGGCCGGGTGGCGATTACCAGTGGAGGGATGTCTTTGGCGGCGGTTGCGGCTTCGCCATGGCCATTTCTGATTCTGGGAAGCTGATTACATGGGGTTCAACAGATGATCTAGGTCAGAGCTATGTGACATCTGGGAAGCATGGGGAAACGCCGGAGCCTTTTCCTCTTCCAGCTGAAGTTTCTCTAGTAAAGGCAGCTGCAGGTTGGGCGCATTGTGTTGCAGTTACAGATATGGGAGCAGTTTACACATGGGGATGGAAAGAGTGTGTTCCCTCTGGGAAGGTCATTGCGGAACCATCTATGGGTACAAACTTTGAGAAGGATGTAATGGAAAGGCAGTGTTCTTTCTTGACAGACCAAGTTAGCCCTCGTTCTCAAGGCTCACGATCCAGTAGCGGAACAATGTCTGCTACTGAAGCTCGGGGAGCTGGAGAAGAATATTCAAAGCGAAGAAGAGTATCTTCCGCAAAGCAAGCAGGTGAAAGTTCATCATCTGGTGATGAAACCCTCTCGGCATTGCCCTATCTTGTGGCATTGAACCCAGGAGTTAGAATAGCCAATGTTGCTGCTGGAGGACGCCATACTCTAGCATTGTCAGACATGGGACAGGTCTGGGGATGGGGATATGGAGGTGAAGGGCAGCTCGGTCTGGGCTCACGGATACGTATGGTGTCCTCCCCTCATCCTATACCTTGTGTCAAGTCCTCTTCTTATGGCATCATGAATCCAGATGAACACAGTTTCGGAGTACCTGGAAATTATGTGAAAGGGATTGCCTGTGGAGGTCGACATAGTGCAGTGATCACAGATGCTGGAGCACTACTTACTTTCGGTTGGGGGCTTTATGGACAGTGTGGACAAGGAAGTACAGATGATGAACTAAGCCCAATTTGTGTATCTTCATTACTTGGTATCCGGATAGAGAGTGTTGCTGCAGGACTGTGGCATACTGTCTGCATTTCAGCTGATGGTGATGTTTATGCATTTGGGGGGAATCAGTTTGGGCAGTTGGGAACTGGAGCTGATCAAGCTGAGACTATTCCGAGGCTTTTGGATGCTCCTGTTCTGGAAAATGTGAATGCAAAAATTGTTTCCTGTGGAGCTCGTCATAGTTGCATTATCACCGAGGATGCAAAAGTGTTTGGCTGGGGATGGAACAAGTATGGCCAGCTTGGCTTAGGCGACGTGATAGACCGCAACATTCCAGCCCAAGTCATGATCGATGGATGTGTCCCCAAAAACGTGGCATGTGGCTGGTGGCATACCTTGCTTCTGGCCGAACTACCGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

473

Amino Acids

49.7

Weight (kDa)

5.12

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 3 - 377 9.1e-63 RCC1-like domain
RCC1_2 PF13540 139 - 168 1.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 156 - 222 6.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 226 - 274 2.5e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 261 - 290 5.9e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 277 - 328 1.3e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 318 - 344 6.4e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 333 - 379 5.3e-13 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 2 cut(s) 317, 593
AccI GTMKAC 1 cut(s) 913
AccIII TCCGGA 1 cut(s) 1041
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 3 cut(s) 186, 1291, 1401
AcuI CTGAAG 2 cut(s) 321, 561
AcyI GRCGYC 1 cut(s) 703
AfaI GTAC 3 cut(s) 433, 876, 994
AfiI CCNNNNNNNGG 2 cut(s) 151, 428
AgsI TTSAA 3 cut(s) 225, 570, 664
AjiI CACGTC 1 cut(s) 1312
AjnI CCWGG 2 cut(s) 667, 877
Alw21I GWGCWC 3 cut(s) 88, 946, 1231
Alw26I GTCTC 2 cut(s) 30, 1154
AlwI GGATC 1 cut(s) 507
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 1 cut(s) 546
Aor13HI TCCGGA 1 cut(s) 1041
AoxI GGCC 4 cut(s) 128, 186, 1291, 1401
ApeKI GCWGC 5 cut(s) 320, 323, 692, 766, 1058
AspLEI GCGC 1 cut(s) 337
AsuC2I CCSGG 2 cut(s) 81, 132
AsuHPI GGTGA 5 cut(s) 617, 635, 770, 1109, 1240
AvaI CYCGRG 1 cut(s) 546
BalI TGGCCA 2 cut(s) 188, 1293
BanII GRGCYC 3 cut(s) 88, 782, 1231
Bbv12I GWGCWC 3 cut(s) 88, 946, 1231
BbvI GCAGC 5 cut(s) 310, 332, 679, 778, 1045
BccI CCATC 6 cut(s) 375, 430, 738, 1088, 1272, 1343
BceAI ACGGC 2 cut(s) 25, 58
BciT130I CCWGG 2 cut(s) 669, 879
BciVI GTATCC 2 cut(s) 780, 1049
BclI TGATCA 2 cut(s) 927, 1150
BcnI CCSGG 2 cut(s) 81, 132
BcoDI GTCTC 2 cut(s) 30, 1154
BfaI CTAG 3 cut(s) 236, 311, 713
BfmI CTRYAG 2 cut(s) 324, 1059
BfuAI ACCTGC 2 cut(s) 317, 593
BfuI GTATCC 2 cut(s) 780, 1049
BglI GCCNNNNNGGC 1 cut(s) 1299
BisI GCNGC 9 cut(s) 92, 104, 166, 175, 321, 324, 693, 767, 1059
BlsI GCNGC 9 cut(s) 93, 105, 167, 176, 322, 325, 694, 768, 1060
Bme1390I CCNGG 4 cut(s) 81, 132, 669, 879
BmeT110I CYCGRG 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 1312
BmiI GGNNCC 2 cut(s) 282, 420
BmrFI CCNGG 4 cut(s) 81, 132, 669, 879
BmsI GCATC 4 cut(s) 852, 925, 1171, 1246
BpmI CTGGAG 4 cut(s) 576, 717, 960, 1164
Bpu10I CCTNAGC 1 cut(s) 1303
BpuEI CTTGAG 1 cut(s) 486
BpuMI CCSGG 2 cut(s) 81, 132
Bsa29I ATCGAT 1 cut(s) 1347
BsaAI YACGTR 1 cut(s) 791
BsaHI GRCGYC 1 cut(s) 703
BsaJI CCNNGG 4 cut(s) 183, 667, 1169, 1251
BsaWI WCCGGW 1 cut(s) 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 428
Bse1I ACTGG 3 cut(s) 145, 516, 1147
Bse3DI GCAATG 2 cut(s) 411, 641
BseAI TCCGGA 1 cut(s) 1041
BseBI CCWGG 2 cut(s) 669, 879
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 4 cut(s) 183, 667, 1169, 1251
BseGI GGATG 9 cut(s) 160, 386, 454, 749, 808, 1186, 1261, 1283, 1358
BseLI CCNNNNNNNGG 2 cut(s) 151, 428
BseMI GCAATG 2 cut(s) 411, 641
BseMII CTCAG 1 cut(s) 1149
BseNI ACTGG 3 cut(s) 145, 516, 1147
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 5 cut(s) 310, 332, 679, 778, 1045
BseYI CCCAGC 1 cut(s) 1272
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 4 cut(s) 130, 188, 1293, 1403
BshVI ATCGAT 1 cut(s) 1347
BsiHKAI GWGCWC 3 cut(s) 88, 946, 1231
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 4 cut(s) 80, 131, 278, 1042
BslFI GGGAC 2 cut(s) 744, 1343
BslI CCNNNNNNNGG 2 cut(s) 151, 428
BsmAI GTCTC 2 cut(s) 30, 1154
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 2 cut(s) 744, 1343
BsmI GAATGC 1 cut(s) 1210
BsnI GGCC 4 cut(s) 130, 188, 1293, 1403
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 4 cut(s) 88, 782, 946, 1231
Bsp13I TCCGGA 1 cut(s) 1041
Bsp143I GATC 5 cut(s) 232, 512, 927, 1150, 1344
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 4 cut(s) 130, 188, 1293, 1403
BspCNI CTCAG 1 cut(s) 1150
BspDI ATCGAT 1 cut(s) 1347
BspEI TCCGGA 1 cut(s) 1041
BspHI TCATGA 2 cut(s) 846, 1341
BspLI GGNNCC 2 cut(s) 282, 420
BspMAI CTGCAG 2 cut(s) 328, 1063
BspMI ACCTGC 2 cut(s) 317, 593
BspPI GGATC 1 cut(s) 507
BsrDI GCAATG 2 cut(s) 411, 641
BsrI ACTGG 3 cut(s) 145, 516, 1147
BssECI CCNNGG 4 cut(s) 183, 667, 1169, 1251
BssMI GATC 5 cut(s) 232, 512, 927, 1150, 1344
BssNI GRCGYC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 2 cut(s) 669, 879
Bst4CI ACNGT 5 cut(s) 121, 866, 978, 1068, 1078
Bst6I CTCTTC 3 cut(s) 297, 574, 838
BstACI GRCGYC 1 cut(s) 703
BstAPI GCANNNNNTGC 1 cut(s) 1393
BstBAI YACGTR 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 600, 1295
BstDEI CTNAG 3 cut(s) 1007, 1158, 1303
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 9 cut(s) 160, 386, 454, 749, 808, 1186, 1261, 1283, 1358
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 5 cut(s) 235, 515, 930, 1153, 1347
BstMAI GTCTC 2 cut(s) 30, 1154
BstMBI GATC 5 cut(s) 232, 512, 927, 1150, 1344
BstNI CCWGG 2 cut(s) 669, 879
BstNSI RCATGY 1 cut(s) 1377
BstSCI CCNGG 4 cut(s) 79, 130, 667, 877
BstSFI CTRYAG 2 cut(s) 324, 1059
BstSNI TACGTA 1 cut(s) 791
BstV1I GCAGC 5 cut(s) 310, 332, 679, 778, 1045
BstXI CCANNNNNNTGG 1 cut(s) 1369
Bsu15I ATCGAT 1 cut(s) 1347
BsuI GTATCC 2 cut(s) 780, 1049
BsuRI GGCC 4 cut(s) 130, 188, 1293, 1403
BsuTUI ATCGAT 1 cut(s) 1347
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 1312
BtsCI GGATG 9 cut(s) 160, 386, 454, 749, 808, 1186, 1261, 1283, 1358
BtsI GCAGTG 2 cut(s) 470, 930
BtsIMutI CAGTG 4 cut(s) 152, 470, 930, 983
BveI ACCTGC 2 cut(s) 317, 593
Cac8I GCNNGC 2 cut(s) 600, 1295
CaiI CAGNNNCTG 1 cut(s) 938
CciI TCATGA 2 cut(s) 846, 1341
CfoI GCGC 1 cut(s) 337
ClaI ATCGAT 1 cut(s) 1347
CseI GACGC 1 cut(s) 711
Csp6I GTAC 3 cut(s) 432, 875, 993
CviAII CATG 9 cut(s) 64, 184, 216, 266, 375, 727, 847, 1342, 1374
CviQI GTAC 3 cut(s) 432, 875, 993
DdeI CTNAG 3 cut(s) 1007, 1158, 1303
DpnI GATC 5 cut(s) 234, 514, 929, 1152, 1346
DpnII GATC 5 cut(s) 232, 512, 927, 1150, 1344
EaeI YGGCCR 3 cut(s) 186, 1291, 1401
Eam1104I CTCTTC 3 cut(s) 297, 574, 838
EarI CTCTTC 3 cut(s) 297, 574, 838
EciI GGCGGA 1 cut(s) 27
Ecl136II GAGCTC 2 cut(s) 86, 1229
Eco105I TACGTA 1 cut(s) 791
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 3 cut(s) 88, 782, 1231
Eco32I GATATC 1 cut(s) 74
Eco53kI GAGCTC 2 cut(s) 86, 1229
Eco57I CTGAAG 2 cut(s) 321, 561
Eco88I CYCGRG 1 cut(s) 546
EcoICRI GAGCTC 2 cut(s) 86, 1229
EcoRII CCWGG 2 cut(s) 667, 877
EcoRV GATATC 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 1111
EcoT38I GRGCYC 3 cut(s) 88, 782, 1231
ErhI CCWWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 9 cut(s) 67, 187, 219, 269, 378, 730, 850, 1345, 1377
FalI AAGNNNNNCTT 2 cut(s) 571, 603
FaqI GGGAC 2 cut(s) 744, 1343
FatI CATG 9 cut(s) 63, 183, 215, 265, 374, 726, 846, 1341, 1373
FauI CCCGC 1 cut(s) 38
FbaI TGATCA 2 cut(s) 927, 1150
FblI GTMKAC 1 cut(s) 913
Fnu4HI GCNGC 9 cut(s) 92, 104, 166, 175, 321, 324, 693, 767, 1059
FokI GGATG 9 cut(s) 167, 393, 461, 756, 795, 1193, 1268, 1290, 1365
FriOI GRGCYC 3 cut(s) 88, 782, 1231
Fsp4HI GCNGC 9 cut(s) 92, 104, 166, 175, 321, 324, 693, 767, 1059
FspBI CTAG 3 cut(s) 236, 311, 713
GlaI GCGC 1 cut(s) 336
GluI GCNGC 9 cut(s) 92, 104, 166, 175, 321, 324, 693, 767, 1059
GsaI CCCAGC 1 cut(s) 1276
GsuI CTGGAG 4 cut(s) 576, 717, 960, 1164
HaeIII GGCC 4 cut(s) 130, 188, 1293, 1403
HapII CCGG 4 cut(s) 80, 131, 278, 1042
HgaI GACGC 1 cut(s) 711
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 703
Hin1II CATG 9 cut(s) 67, 187, 219, 269, 378, 730, 850, 1345, 1377
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 914
HindII GTYRAC 1 cut(s) 914
HinfI GANTC 3 cut(s) 197, 850, 1120
HpaII CCGG 4 cut(s) 80, 131, 278, 1042
HphI GGTGA 5 cut(s) 617, 635, 770, 1109, 1240
Hpy166II GTNNAC 3 cut(s) 370, 914, 983
Hpy188I TCNGA 5 cut(s) 196, 243, 724, 872, 1170
Hpy188III TCNNGA 7 cut(s) 475, 510, 847, 854, 1042, 1193, 1342
Hpy8I GTNNAC 3 cut(s) 370, 914, 983
Hpy99I CGWCG 3 cut(s) 44, 121, 1313
HpyAV CCTTC 4 cut(s) 46, 400, 439, 755
HpyCH4III ACNGT 5 cut(s) 121, 866, 978, 1068, 1078
HpyCH4IV ACGT 3 cut(s) 790, 1311, 1368
HpyCH4V TGCA 9 cut(s) 326, 347, 923, 1061, 1083, 1109, 1208, 1242, 1259
HpyF3I CTNAG 3 cut(s) 1007, 1158, 1303
HpySE526I ACGT 3 cut(s) 790, 1311, 1368
Hsp92I GRCGYC 1 cut(s) 703
Hsp92II CATG 9 cut(s) 67, 187, 219, 269, 378, 730, 850, 1345, 1377
HspAI GCGC 1 cut(s) 335
Kpn2I TCCGGA 1 cut(s) 1041
Ksp22I TGATCA 2 cut(s) 927, 1150
Kzo9I GATC 5 cut(s) 232, 512, 927, 1150, 1344
LmnI GCTCC 8 cut(s) 83, 280, 362, 551, 941, 1145, 1189, 1226
Lsp1109I GCAGC 5 cut(s) 310, 332, 679, 778, 1045
LweI GCATC 4 cut(s) 852, 925, 1171, 1246
MaeI CTAG 3 cut(s) 236, 311, 713
MaeII ACGT 3 cut(s) 790, 1311, 1368
MaeIII GTNAC 2 cut(s) 250, 349
MalI GATC 5 cut(s) 234, 514, 929, 1152, 1346
MboI GATC 5 cut(s) 232, 512, 927, 1150, 1344
MboII GAAGA 8 cut(s) 68, 284, 572, 579, 588, 591, 825, 1017
MhlI GDGCHC 4 cut(s) 88, 782, 946, 1231
MlsI TGGCCA 2 cut(s) 188, 1293
MluCI AATT 3 cut(s) 883, 1014, 1212
MluNI TGGCCA 2 cut(s) 188, 1293
Mox20I TGGCCA 2 cut(s) 188, 1293
Mph1103I ATGCAT 1 cut(s) 1111
MroI TCCGGA 1 cut(s) 1041
MscI TGGCCA 2 cut(s) 188, 1293
MslI CAYNNNNRTG 1 cut(s) 379
Msp20I TGGCCA 2 cut(s) 188, 1293
MspA1I CMGCKG 3 cut(s) 299, 323, 1091
MspI CCGG 4 cut(s) 80, 131, 278, 1042
MspR9I CCNGG 4 cut(s) 81, 132, 669, 879
Mva1269I GAATGC 1 cut(s) 1210
MvaI CCWGG 2 cut(s) 669, 879
NciI CCSGG 2 cut(s) 81, 132
NcoI CCATGG 1 cut(s) 183
NdeII GATC 5 cut(s) 232, 512, 927, 1150, 1344
NlaIII CATG 9 cut(s) 67, 187, 219, 269, 378, 730, 850, 1345, 1377
NlaIV GGNNCC 2 cut(s) 282, 420
NmeAIII GCCGAG 1 cut(s) 617
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 1111
NspI RCATGY 1 cut(s) 1377
PagI TCATGA 2 cut(s) 846, 1341
PaqCI CACCTGC 1 cut(s) 593
PctI GAATGC 1 cut(s) 1210
PfeI GAWTC 3 cut(s) 197, 850, 1120
PfoI TCCNGGA 1 cut(s) 79
PkrI GCNGC 9 cut(s) 93, 105, 167, 176, 322, 325, 694, 768, 1060
Ppu21I YACGTR 1 cut(s) 791
Psp124BI GAGCTC 2 cut(s) 88, 1231
Psp6I CCWGG 2 cut(s) 667, 877
PspFI CCCAGC 1 cut(s) 1272
PspGI CCWGG 2 cut(s) 667, 877
PspN4I GGNNCC 2 cut(s) 282, 420
PstI CTGCAG 2 cut(s) 328, 1063
PstNI CAGNNNCTG 1 cut(s) 938
PvuII CAGCTG 3 cut(s) 299, 323, 1091
RsaI GTAC 3 cut(s) 433, 876, 994
RsaNI GTAC 3 cut(s) 432, 875, 993
RseI CAYNNNNRTG 1 cut(s) 379
SacI GAGCTC 2 cut(s) 88, 1231
SalI GTCGAC 1 cut(s) 912
SatI GCNGC 9 cut(s) 92, 104, 166, 175, 321, 324, 693, 767, 1059
Sau3AI GATC 5 cut(s) 232, 512, 927, 1150, 1344
ScrFI CCNGG 4 cut(s) 81, 132, 669, 879
SduI GDGCHC 4 cut(s) 88, 782, 946, 1231
SfaNI GCATC 4 cut(s) 852, 925, 1171, 1246
SfcI CTRYAG 2 cut(s) 324, 1059
SmiMI CAYNNNNRTG 1 cut(s) 379
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
SnaBI TACGTA 1 cut(s) 791
Sse9I AATT 3 cut(s) 883, 1014, 1212
SspI AATATT 1 cut(s) 566
SspMI CTAG 3 cut(s) 236, 311, 713
SstI GAGCTC 2 cut(s) 88, 1231
StyD4I CCNGG 4 cut(s) 79, 130, 667, 877
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 5 cut(s) 121, 866, 978, 1068, 1078
TaiI ACGT 3 cut(s) 793, 1314, 1371
TaqI TCGA 2 cut(s) 913, 1347
TaqII GACCGA 1 cut(s) 761
TasI AATT 3 cut(s) 883, 1014, 1212
TatI WGTACW 1 cut(s) 992
TauI GCSGC 4 cut(s) 94, 106, 168, 177
TfiI GAWTC 3 cut(s) 197, 850, 1120
TscAI CASTG 4 cut(s) 152, 470, 930, 983
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 5 cut(s) 320, 323, 692, 766, 1058
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 8 cut(s) 17, 52, 603, 642, 863, 873, 1017, 1017
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 4 cut(s) 152, 470, 930, 983
XceI RCATGY 1 cut(s) 1377
XmiI GTMKAC 1 cut(s) 913
XspI CTAG 3 cut(s) 236, 311, 713
Zsp2I ATGCAT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.