pycom07g19070

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004604
Physical Location & Seq
Forward (+)
21428048 .. 21432855
4808 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19070.1

Sequence Viewer

Length: 1740 bp
ATGGACAGACGGCCACGCCAGCCCCGAACATTGCTCTCTGTAGTTAAGGGAGCCCGACCTTCCAGAAACTTCCGGGAACTACTGGCTTGCAGCGGCCGAGGCATCTTGGGGTCCAAAACGAGCCACGTGTCCACACAGCCACGCGGGAATCCAACGTGGCGTCCCCATTTTCCTGTATTTGATACGGCCCCATTTTTAGCAACCCACTCAAAAAACTTAAAAGAAAATTCCGAAAATTCCGAAAACGAAATACGAAAAGGATTAGGAATCCGTTTCCGTTCCCTATTTTTCGGATTTTATCGAGCGATGAATGCGGAGGGAATTACGGAGGGAGAGAAAGTGAAGATGGAAGAGGGGAAAGAGGAGGTGGTGTTCATGTGGGGATATCTTCCCGGAGCTCTGCCCCAGAGATCGCCGCTGTTGTCGCCGACGGTCGTGCGGACGGCCGACGCCGAGTACGCGTGGAAGGATGTATGCGGTGGCGGCTGCGGCTTCGCCATGGCCATATCTGAGCCTGGAAAGCTTATTACATGGGGCTCAACAGATGATCTAGGCCAAAGCTATGTGACATCTGGGATGCACGGGGAAAACCCGGAGCCTTTTCCTCTTCCGAATGATGCTTGTATAGTACAGGCGGCCGCGGGTTGGGCCCATTGTGTTGCAGTCACAGATAATGGAGGAGTTTACACATGGGGATGGAAAGAGTGTGTTCCATCCGGGAAGGTCTTTGGGGAGCAATCTATGGGTCCAGACGGTGTAAAGGATGCCTATGAAAGGCAGAATTCTTTTATGACTGAGCAAGTTAGCCCTCGCTCTCAAGGCTCACGATCCACTGCTGCAGCAGTTTCTGCTAGCGAAGAAAGTACAAAGCGAAGAAAATTGTCGTCAGCAAAACAAGCGGCTGAAAGTTCATCATCTGGTGATGAACCTCCGTCAGCATTGCCATGTCTTGTGGCATTTAACCCGGGAGTTAGAATAGCCAGTGTTGCTGCTGGAGGGCGCCATAGTCTGGCATTATCAGATATAGGACAAGTGTGGGGATGGGGCTATGGAGGTGAAGGGCAGCTTGGTCTGGGCTCCCGCATACGTATGGTGTCCTCTCCTCATCCTGTACCTTGCATTGAGTCCTCTTATGGAAAAGATAGATCTGCAGCACTGTCTCGAGGAACCATGGGTTCAGACGGATTTGGTGTTAGGGTTCCTGGAAATTATGTGAAGGGGATTGCCTGTGGAGGGCGACATAGTGTAGTAATCACAGATGCTGGAGCAGTACTTGCTTTTGGTTGGGGGCTGTATGGACAGTGTGGGCAAGGAAGCACAGATGACGAGTTAAGCCCGATTTGTGTATCTTCATTACTGGGTATCCGGATAGAGGGTGTCGCGGCAGGACTGTGGCACACTGTCTGCATTTCAGCAGATGGCGATGTTTATGCATTTGGTGGGAATCAGTTTGGACAGCTGGGAACCGGAGCTGATCAAGCTGAGACGATACCAAGACTTTTGGATGCTCCAATTCTGGAAAATGAGAATGCAAAGATTGTTTCCTGTGGGGCGCGTCATAGTGCCATAATCACGGAGGATGGGAAAGTTTTCGGCTGGGGATGGAACAAGTATGGTCAGCTTGGCCTGGGTGATGTGATCGACCGCAACATTCCGGCTCAAGTCACGATCGACGGATGTGTGCCGAAGAATGTGGCATGCGGCTGGTGGCATACCTTACTGATTGCTGAACCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

580

Amino Acids

61.57

Weight (kDa)

6.44

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 136 - 240 8.8e-14 RCC1-like domain
RCC1_2 PF13540 209 - 233 3.9e-06 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 312 - 573 2.4e-54 RCC1-like domain
RCC1_2 PF13540 326 - 355 2e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 405 - 434 3.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 422 - 470 1.2e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 459 - 486 6.1e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 473 - 524 5.5e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 514 - 540 2.5e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 527 - 575 5.1e-14 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 999
AccB7I CCANNNNNTGG 1 cut(s) 1009
AccII CGCG 5 cut(s) 144, 461, 641, 1382, 1555
AccIII TCCGGA 1 cut(s) 1365
AclWI GGATC 1 cut(s) 822
AcoI YGGCCR 5 cut(s) 11, 94, 444, 501, 636
AcsI RAATTY 3 cut(s) 226, 235, 781
AcvI CACGTG 1 cut(s) 127
AcyI GRCGYC 3 cut(s) 160, 450, 1000
AfaI GTAC 5 cut(s) 458, 630, 865, 1113, 1272
AfiI CCNNNNNNNGG 5 cut(s) 645, 774, 1009, 1233, 1372
AflIII ACRYGT 2 cut(s) 126, 459
AjnI CCWGG 3 cut(s) 514, 1201, 1626
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
AluBI AGCT 8 cut(s) 398, 523, 561, 1066, 1459, 1472, 1481, 1621
AluI AGCT 8 cut(s) 398, 523, 561, 1066, 1459, 1472, 1481, 1621
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 2 cut(s) 1164, 1478
AlwI GGATC 1 cut(s) 822
AlwNI CAGNNNCTG 2 cut(s) 848, 1262
Ama87I CYCGRG 2 cut(s) 964, 1161
Aor13HI TCCGGA 1 cut(s) 1365
AoxI GGCC 9 cut(s) 11, 94, 186, 444, 501, 553, 636, 648, 1624
ApaI GGGCCC 1 cut(s) 652
ApeKI GCWGC 7 cut(s) 90, 486, 836, 839, 989, 1063, 1151
ApoI RAATTY 3 cut(s) 226, 235, 781
Asp700I GAANNNNTTC 1 cut(s) 1589
AspLEI GCGC 2 cut(s) 1002, 1555
AspS9I GGNCC 5 cut(s) 111, 187, 648, 649, 746
AsuC2I CCSGG 6 cut(s) 74, 393, 593, 718, 965, 966
AsuHPI GGTGA 3 cut(s) 932, 1067, 1643
AsuNHI GCTAGC 1 cut(s) 851
AvaI CYCGRG 2 cut(s) 964, 1161
AvaII GGWCC 2 cut(s) 111, 746
BaeGI GKGCMC 1 cut(s) 652
BalI TGGCCA 1 cut(s) 503
BanI GGYRCC 1 cut(s) 999
BanII GRGCYC 5 cut(s) 55, 400, 539, 652, 1079
BbrPI CACGTG 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 7 cut(s) 102, 473, 823, 851, 976, 1075, 1163
BccI CCATC 7 cut(s) 340, 690, 721, 1035, 1412, 1574, 1596
BceAI ACGGC 3 cut(s) 26, 201, 459
BcgI CGANNNNNNTGC 4 cut(s) 418, 452, 1412, 1446
BciT130I CCWGG 3 cut(s) 516, 1203, 1628
BciVI GTATCC 1 cut(s) 1373
BclI TGATCA 1 cut(s) 1474
BcnI CCSGG 6 cut(s) 74, 393, 593, 718, 965, 966
BcoDI GTCTC 2 cut(s) 1164, 1478
BfaI CTAG 2 cut(s) 551, 852
BfmI CTRYAG 3 cut(s) 39, 837, 1149
BfoI RGCGCY 1 cut(s) 1003
BfuI GTATCC 1 cut(s) 1373
BglII AGATCT 1 cut(s) 1145
BmcAI AGTACT 1 cut(s) 1272
Bme1390I CCNGG 9 cut(s) 74, 393, 516, 593, 718, 965, 966, 1203, 1628
Bme18I GGWCC 2 cut(s) 111, 746
BmeT110I CYCGRG 2 cut(s) 964, 1161
BmgT120I GGNCC 5 cut(s) 111, 187, 648, 649, 746
BmrFI CCNGG 9 cut(s) 74, 393, 516, 593, 718, 965, 966, 1203, 1628
BmrI ACTGGG 1 cut(s) 1367
BmsI GCATC 6 cut(s) 111, 567, 607, 754, 1249, 1495
BmtI GCTAGC 1 cut(s) 855
BmuI ACTGGG 1 cut(s) 1367
BpmI CTGGAG 2 cut(s) 1014, 1284
BpuEI CTTGAG 2 cut(s) 801, 1644
BpuMI CCSGG 6 cut(s) 74, 393, 593, 718, 965, 966
BsaAI YACGTR 2 cut(s) 127, 1088
BsaHI GRCGYC 3 cut(s) 160, 450, 1000
BsaJI CCNNGG 6 cut(s) 97, 498, 639, 964, 1170, 1627
BsaWI WCCGGW 2 cut(s) 1365, 1466
BsaXI ACNNNNNCTCC 4 cut(s) 324, 354, 356, 386
Bsc4I CCNNNNNNNGG 5 cut(s) 645, 774, 1009, 1233, 1372
Bse1I ACTGG 3 cut(s) 87, 981, 1362
Bse3DI GCAATG 2 cut(s) 29, 938
BseAI TCCGGA 1 cut(s) 1365
BseBI CCWGG 3 cut(s) 516, 1203, 1628
BseDI CCNNGG 6 cut(s) 97, 498, 639, 964, 1170, 1627
BseLI CCNNNNNNNGG 5 cut(s) 645, 774, 1009, 1233, 1372
BseMI GCAATG 2 cut(s) 29, 938
BseMII CTCAG 3 cut(s) 501, 786, 1473
BseNI ACTGG 3 cut(s) 87, 981, 1362
BseRI GAGGAG 3 cut(s) 377, 693, 1092
BseSI GKGCMC 1 cut(s) 652
BseX3I CGGCCG 3 cut(s) 94, 444, 636
BseXI GCAGC 7 cut(s) 102, 473, 823, 851, 976, 1075, 1163
BseYI CCCAGC 2 cut(s) 1459, 1596
Bsh1236I CGCG 5 cut(s) 144, 461, 641, 1382, 1555
Bsh1285I CGRYCG 6 cut(s) 97, 435, 447, 639, 1645, 1671
BshFI GGCC 9 cut(s) 13, 96, 188, 446, 503, 555, 638, 650, 1626
BshNI GGYRCC 1 cut(s) 999
BsiEI CGRYCG 6 cut(s) 97, 435, 447, 639, 1645, 1671
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 2 cut(s) 964, 1161
BsiSI CCGG 8 cut(s) 73, 393, 593, 717, 965, 1366, 1467, 1655
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 5 cut(s) 645, 774, 1009, 1233, 1372
BsmAI GTCTC 2 cut(s) 1164, 1478
BsmBI CGTCTC 1 cut(s) 1478
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 2 cut(s) 316, 1534
BsnI GGCC 9 cut(s) 13, 96, 188, 446, 503, 555, 638, 650, 1626
BsoBI CYCGRG 2 cut(s) 964, 1161
Bsp120I GGGCCC 1 cut(s) 648
Bsp1286I GDGCHC 5 cut(s) 55, 400, 539, 652, 1079
Bsp13I TCCGGA 1 cut(s) 1365
Bsp143I GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
Bsp19I CCATGG 2 cut(s) 498, 1170
BspANI GGCC 9 cut(s) 13, 96, 188, 446, 503, 555, 638, 650, 1626
BspCNI CTCAG 3 cut(s) 502, 787, 1474
BspEI TCCGGA 1 cut(s) 1365
BspFNI CGCG 5 cut(s) 144, 461, 641, 1382, 1555
BspMAI CTGCAG 2 cut(s) 841, 1153
BspOI GCTAGC 1 cut(s) 855
BspPI GGATC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 999
BsrDI GCAATG 2 cut(s) 29, 938
BsrI ACTGG 3 cut(s) 87, 981, 1362
BssECI CCNNGG 6 cut(s) 97, 498, 639, 964, 1170, 1627
BssMI GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
BssNI GRCGYC 3 cut(s) 160, 450, 1000
BssT1I CCWWGG 2 cut(s) 498, 1170
Bst2UI CCWGG 3 cut(s) 516, 1203, 1628
Bst4CI ACNGT 6 cut(s) 433, 755, 1158, 1302, 1392, 1402
Bst6I CTCTTC 2 cut(s) 345, 612
BstACI GRCGYC 3 cut(s) 160, 450, 1000
BstAPI GCANNNNNTGC 2 cut(s) 848, 1274
BstBAI YACGTR 2 cut(s) 127, 1088
BstC8I GCNNGC 4 cut(s) 20, 88, 853, 1699
BstDEI CTNAG 3 cut(s) 510, 795, 1482
BstDSI CCRYGG 3 cut(s) 498, 639, 1170
BstENI CCTNNNNNAGG 1 cut(s) 772
BstFNI CGCG 5 cut(s) 144, 461, 641, 1382, 1555
BstH2I RGCGCY 1 cut(s) 1003
BstHHI GCGC 2 cut(s) 1002, 1555
BstKTI GATC 7 cut(s) 413, 550, 830, 1148, 1477, 1641, 1671
BstMAI GTCTC 2 cut(s) 1164, 1478
BstMBI GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
BstMCI CGRYCG 6 cut(s) 97, 435, 447, 639, 1645, 1671
BstNI CCWGG 3 cut(s) 516, 1203, 1628
BstNSI RCATGY 1 cut(s) 1701
BstSCI CCNGG 9 cut(s) 72, 391, 514, 591, 716, 963, 964, 1201, 1626
BstSFI CTRYAG 3 cut(s) 39, 837, 1149
BstSLI GKGCMC 1 cut(s) 652
BstSNI TACGTA 1 cut(s) 1088
BstUI CGCG 5 cut(s) 144, 461, 641, 1382, 1555
BstV1I GCAGC 7 cut(s) 102, 473, 823, 851, 976, 1075, 1163
BstX2I RGATCY 1 cut(s) 1145
BstYI RGATCY 1 cut(s) 1145
BstZI CGGCCG 3 cut(s) 94, 444, 636
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 9 cut(s) 13, 96, 188, 446, 503, 555, 638, 650, 1626
BtgI CCRYGG 3 cut(s) 498, 639, 1170
BtgZI GCGATG 2 cut(s) 320, 1437
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 5 cut(s) 831, 988, 1154, 1307, 1398
Cac8I GCNNGC 4 cut(s) 20, 88, 853, 1699
CaiI CAGNNNCTG 2 cut(s) 848, 1262
CciNI GCGGCCGC 1 cut(s) 636
CfoI GCGC 2 cut(s) 1002, 1555
Cfr13I GGNCC 5 cut(s) 111, 187, 648, 649, 746
Cfr42I CCGCGG 1 cut(s) 642
Cfr9I CCCGGG 1 cut(s) 964
CseI GACGC 3 cut(s) 149, 458, 1544
Csp6I GTAC 5 cut(s) 457, 629, 864, 1112, 1271
CviAII CATG 7 cut(s) 376, 499, 531, 690, 945, 1171, 1698
CviQI GTAC 5 cut(s) 457, 629, 864, 1112, 1271
DdeI CTNAG 3 cut(s) 510, 795, 1482
DinI GGCGCC 1 cut(s) 1001
DpnI GATC 7 cut(s) 412, 549, 829, 1147, 1476, 1640, 1670
DpnII GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
EaeI YGGCCR 5 cut(s) 11, 94, 444, 501, 636
EagI CGGCCG 3 cut(s) 94, 444, 636
Eam1104I CTCTTC 2 cut(s) 345, 612
EarI CTCTTC 2 cut(s) 345, 612
Ecl136II GAGCTC 1 cut(s) 398
EclXI CGGCCG 3 cut(s) 94, 444, 636
Eco105I TACGTA 1 cut(s) 1088
Eco130I CCWWGG 2 cut(s) 498, 1170
Eco24I GRGCYC 5 cut(s) 55, 400, 539, 652, 1079
Eco32I GATATC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 111, 746
Eco52I CGGCCG 3 cut(s) 94, 444, 636
Eco53kI GAGCTC 1 cut(s) 398
Eco72I CACGTG 1 cut(s) 127
Eco88I CYCGRG 2 cut(s) 964, 1161
EcoICRI GAGCTC 1 cut(s) 398
EcoNI CCTNNNNNAGG 1 cut(s) 772
EcoRI GAATTC 1 cut(s) 781
EcoRII CCWGG 3 cut(s) 514, 1201, 1626
EcoRV GATATC 1 cut(s) 386
EcoT14I CCWWGG 2 cut(s) 498, 1170
EcoT22I ATGCAT 1 cut(s) 1435
EcoT38I GRGCYC 5 cut(s) 55, 400, 539, 652, 1079
EgeI GGCGCC 1 cut(s) 1001
EheI GGCGCC 1 cut(s) 1001
ErhI CCWWGG 2 cut(s) 498, 1170
Esp3I CGTCTC 1 cut(s) 1478
FaeI CATG 7 cut(s) 379, 502, 534, 693, 948, 1174, 1701
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 1 cut(s) 147
FatI CATG 7 cut(s) 375, 498, 530, 689, 944, 1170, 1697
FauI CCCGC 3 cut(s) 137, 634, 1088
FbaI TGATCA 1 cut(s) 1474
FriOI GRGCYC 5 cut(s) 55, 400, 539, 652, 1079
FspBI CTAG 2 cut(s) 551, 852
GlaI GCGC 2 cut(s) 1001, 1554
GsaI CCCAGC 2 cut(s) 1463, 1600
GsuI CTGGAG 2 cut(s) 1014, 1284
HaeII RGCGCY 1 cut(s) 1003
HaeIII GGCC 9 cut(s) 13, 96, 188, 446, 503, 555, 638, 650, 1626
HapII CCGG 8 cut(s) 73, 393, 593, 717, 965, 1366, 1467, 1655
HgaI GACGC 3 cut(s) 149, 458, 1544
HhaI GCGC 2 cut(s) 1002, 1555
Hin1I GRCGYC 3 cut(s) 160, 450, 1000
Hin1II CATG 7 cut(s) 379, 502, 534, 693, 948, 1174, 1701
Hin6I GCGC 2 cut(s) 1000, 1553
HinP1I GCGC 2 cut(s) 1000, 1553
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 4 cut(s) 148, 267, 1124, 1444
HpaII CCGG 8 cut(s) 73, 393, 593, 717, 965, 1366, 1467, 1655
HphI GGTGA 3 cut(s) 932, 1067, 1643
Hpy166II GTNNAC 2 cut(s) 132, 685
Hpy188I TCNGA 7 cut(s) 232, 241, 293, 511, 612, 1021, 1180
Hpy188III TCNNGA 7 cut(s) 63, 749, 825, 1161, 1366, 1517, 1666
Hpy8I GTNNAC 2 cut(s) 132, 685
Hpy99I CGWCG 3 cut(s) 433, 452, 1676
HpyAV CCTTC 5 cut(s) 69, 460, 715, 1052, 1210
HpyCH4III ACNGT 6 cut(s) 433, 755, 1158, 1302, 1392, 1402
HpyCH4IV ACGT 3 cut(s) 126, 155, 1087
HpyCH4V TGCA 9 cut(s) 90, 580, 662, 839, 1119, 1151, 1407, 1433, 1532
HpyF3I CTNAG 3 cut(s) 510, 795, 1482
HpySE526I ACGT 3 cut(s) 126, 155, 1087
Hsp92I GRCGYC 3 cut(s) 160, 450, 1000
Hsp92II CATG 7 cut(s) 379, 502, 534, 693, 948, 1174, 1701
HspAI GCGC 2 cut(s) 1000, 1553
KasI GGCGCC 1 cut(s) 999
Kpn2I TCCGGA 1 cut(s) 1365
Ksp22I TGATCA 1 cut(s) 1474
KspI CCGCGG 1 cut(s) 642
Kzo9I GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
LmnI GCTCC 8 cut(s) 50, 395, 595, 733, 1082, 1265, 1469, 1513
Lsp1109I GCAGC 7 cut(s) 102, 473, 823, 851, 976, 1075, 1163
LweI GCATC 6 cut(s) 111, 567, 607, 754, 1249, 1495
MaeI CTAG 2 cut(s) 551, 852
MaeII ACGT 3 cut(s) 126, 155, 1087
MaeIII GTNAC 3 cut(s) 565, 664, 1663
MalI GATC 7 cut(s) 412, 549, 829, 1147, 1476, 1640, 1670
MboI GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
MboII GAAGA 8 cut(s) 355, 362, 380, 599, 869, 885, 1341, 1699
MflI RGATCY 1 cut(s) 1145
MhlI GDGCHC 5 cut(s) 55, 400, 539, 652, 1079
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 7 cut(s) 226, 235, 321, 781, 878, 1207, 1512
MluI ACGCGT 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 503
Mly113I GGCGCC 1 cut(s) 1000
MlyI GAGTC 1 cut(s) 1133
MmeI TCCRAC 1 cut(s) 176
Mox20I TGGCCA 1 cut(s) 503
Mph1103I ATGCAT 1 cut(s) 1435
MroI TCCGGA 1 cut(s) 1365
MroXI GAANNNNTTC 1 cut(s) 1589
MscI TGGCCA 1 cut(s) 503
MseI TTAA 4 cut(s) 45, 218, 960, 1331
MslI CAYNNNNRTG 3 cut(s) 694, 943, 1088
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 4 cut(s) 93, 418, 641, 1459
MspI CCGG 8 cut(s) 73, 393, 593, 717, 965, 1366, 1467, 1655
MspR9I CCNGG 9 cut(s) 74, 393, 516, 593, 718, 965, 966, 1203, 1628
Mva1269I GAATGC 2 cut(s) 316, 1534
MvaI CCWGG 3 cut(s) 516, 1203, 1628
MvnI CGCG 5 cut(s) 144, 461, 641, 1382, 1555
NarI GGCGCC 1 cut(s) 1000
NciI CCSGG 6 cut(s) 74, 393, 593, 718, 965, 966
NcoI CCATGG 2 cut(s) 498, 1170
NdeII GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
NheI GCTAGC 1 cut(s) 851
NlaIII CATG 7 cut(s) 379, 502, 534, 693, 948, 1174, 1701
NmeAIII GCCGAG 2 cut(s) 122, 478
NmuCI GTSAC 3 cut(s) 565, 664, 1663
NotI GCGGCCGC 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 1435
NspI RCATGY 1 cut(s) 1701
PaeI GCATGC 1 cut(s) 1701
PaeR7I CTCGAG 1 cut(s) 1161
PcsI WCGNNNNNNNCGW 1 cut(s) 22
PctI GAATGC 2 cut(s) 316, 1534
PdmI GAANNNNTTC 1 cut(s) 1589
PfeI GAWTC 3 cut(s) 148, 267, 1444
PflMI CCANNNNNTGG 1 cut(s) 1009
PfoI TCCNGGA 4 cut(s) 72, 391, 716, 1201
Ple19I CGATCG 1 cut(s) 1671
PleI GAGTC 1 cut(s) 1132
PluTI GGCGCC 1 cut(s) 1003
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 1 cut(s) 1132
Ppu21I YACGTR 2 cut(s) 127, 1088
Psp124BI GAGCTC 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 514, 1201, 1626
PspCI CACGTG 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 1459, 1596
PspGI CCWGG 3 cut(s) 514, 1201, 1626
PspOMI GGGCCC 1 cut(s) 648
PspPI GGNCC 5 cut(s) 111, 187, 648, 649, 746
PstI CTGCAG 2 cut(s) 841, 1153
PstNI CAGNNNCTG 2 cut(s) 848, 1262
PsuI RGATCY 1 cut(s) 1145
PvuI CGATCG 1 cut(s) 1671
PvuII CAGCTG 1 cut(s) 1459
RsaI GTAC 5 cut(s) 458, 630, 865, 1113, 1272
RsaNI GTAC 5 cut(s) 457, 629, 864, 1112, 1271
RseI CAYNNNNRTG 3 cut(s) 694, 943, 1088
SacI GAGCTC 1 cut(s) 400
SacII CCGCGG 1 cut(s) 642
SaqAI TTAA 4 cut(s) 45, 218, 960, 1331
Sau3AI GATC 7 cut(s) 410, 547, 827, 1145, 1474, 1638, 1668
Sau96I GGNCC 5 cut(s) 111, 187, 648, 649, 746
ScaI AGTACT 1 cut(s) 1272
SchI GAGTC 1 cut(s) 1133
ScrFI CCNGG 9 cut(s) 74, 393, 516, 593, 718, 965, 966, 1203, 1628
SduI GDGCHC 5 cut(s) 55, 400, 539, 652, 1079
SfaNI GCATC 6 cut(s) 111, 567, 607, 754, 1249, 1495
SfcI CTRYAG 3 cut(s) 39, 837, 1149
SfoI GGCGCC 1 cut(s) 1001
Sfr274I CTCGAG 1 cut(s) 1161
Sfr303I CCGCGG 1 cut(s) 642
SgrBI CCGCGG 1 cut(s) 642
SinI GGWCC 2 cut(s) 111, 746
SlaI CTCGAG 1 cut(s) 1161
SmaI CCCGGG 1 cut(s) 966
SmiMI CAYNNNNRTG 3 cut(s) 694, 943, 1088
SmlI CTYRAG 3 cut(s) 816, 1161, 1659
SmoI CTYRAG 3 cut(s) 816, 1161, 1659
SnaBI TACGTA 1 cut(s) 1088
SphI GCATGC 1 cut(s) 1701
Sse9I AATT 7 cut(s) 226, 235, 321, 781, 878, 1207, 1512
SspDI GGCGCC 1 cut(s) 999
SspMI CTAG 2 cut(s) 551, 852
SstI GAGCTC 1 cut(s) 400
StyD4I CCNGG 9 cut(s) 72, 391, 514, 591, 716, 963, 964, 1201, 1626
StyI CCWWGG 2 cut(s) 498, 1170
TaaI ACNGT 6 cut(s) 433, 755, 1158, 1302, 1392, 1402
TaiI ACGT 3 cut(s) 129, 158, 1090
TaqI TCGA 4 cut(s) 301, 1162, 1641, 1671
TasI AATT 7 cut(s) 226, 235, 321, 781, 878, 1207, 1512
TatI WGTACW 3 cut(s) 628, 863, 1270
TauI GCSGC 9 cut(s) 96, 418, 486, 492, 638, 641, 902, 1385, 1704
TfiI GAWTC 3 cut(s) 148, 267, 1444
Tru1I TTAA 4 cut(s) 45, 218, 960, 1331
Tru9I TTAA 4 cut(s) 45, 218, 960, 1331
TscAI CASTG 5 cut(s) 838, 988, 1161, 1307, 1405
TseFI GTSAC 3 cut(s) 565, 664, 1663
TseI GCWGC 7 cut(s) 90, 486, 836, 839, 989, 1063, 1151
Tsp45I GTSAC 3 cut(s) 565, 664, 1663
TspDTI ATGAA 6 cut(s) 323, 364, 786, 900, 939, 1341
TspGWI ACGGA 7 cut(s) 260, 266, 341, 921, 1197, 1589, 1689
TspMI CCCGGG 1 cut(s) 964
TspRI CASTG 5 cut(s) 838, 988, 1161, 1307, 1405
Van91I CCANNNNNTGG 1 cut(s) 1009
VpaK11BI GGWCC 2 cut(s) 111, 746
XagI CCTNNNNNAGG 1 cut(s) 772
XapI RAATTY 3 cut(s) 226, 235, 781
XceI RCATGY 1 cut(s) 1701
XhoI CTCGAG 1 cut(s) 1161
XmaI CCCGGG 1 cut(s) 964
XmnI GAANNNNTTC 1 cut(s) 1589
XspI CTAG 2 cut(s) 551, 852
ZrmI AGTACT 1 cut(s) 1272
Zsp2I ATGCAT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.