Rorug01G0342100

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004604
Physical Location & Seq
Reverse (-)
45994606 .. 46003334
8729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0342100.1

Sequence Viewer

Length: 2718 bp
ATGAAGTTCACCTTGTGTTCATCATCTTCTGAGCTTAGATATCCTGTTGTTGAGGGGTTGCCCAGACGTACAAACAAGAATATACTAGAGCCTGAACCGTCGGTGAAGGTTGATAATTTTGTTAAGAATGTTAATGCTCAAGCATCTAATCATGATACGAGGAAGCTGGTGATGTCTAGCAATTTGATGCCTCAGCACATACAAGAATCACATGTGGGAAAGAAACAACTGGAGGCACCTGGTCGGAAGTTGGAATTTGTGAGGACATTATTGATTGACAACTATGATAGCTACACATACAATATATACCAGGAGCTGTCTGTCATTAATGGCTTACCTCCTGTTGTGGTGCGAAATGATGATTTGACATGGAAAGATGTTTGCCATTACTTGTATGAAGAAAATGCATTTGATAACATTGTTATATCACCTGGACCTGGTTCTCCTACATGCCCAGCGGACATAGGAATATGTCTTCAGGTGCTGCTTGACTGCTGGAATGTCCCTATCTTGGGTGTTTGCCTTGGACATCAGGCTTTAGGTTACGTGCATGGTGCTAAAGTTGTCCACGCATCTGAACCAGTCCATGGACGCTTGAGTGAAATTGAGCACAATGGGTGCGTACTTTTCAATGACATTCCCTCTGGCCACAACTCTGGATTCAAGGTGGTTCGGTACCATTCACTGGTCATAGATGCTGAATCACTCCCAGATGAACTCATTCCTATAGCATGGACCTCTTCCGTGAATGCACTTTCTTTTATTGAGACCAAAGAATCTGATGTTGCCTCTAAATTTGCTGATGGGTCTTTTTCAAGGAAACCAAAAAATGGAAGTTATTCACCTTCCAGCCATTCTGGCAAAGTACAGAGTGAAAGGGTTCTCATGGGCATTATGCATTCTTCCAGGCCTCATTATGGTGTACAGTTCCATCCAGAGAGTGTTGCTACCTGCCATGGAAGGCAAATATTCAAGAATTTTAGAAAAATCACAGAGGATTATTGGCTGAGATCAAGGGCATCATTCATCAACGCACGAAATTTTCCTTTTAATGCACACATGCAGATGCCCCATGTTGGTCAACTATTGACAGACGTTCCAAAACACAGGCAACCACTAAATAATGCAGATGGTCAATTATATAAAAATGCAAATAACAGAAGTTTCTCTGGCATGGTTGATATGGTAAACCTTTTGCATCCAAGCATTACTATTAAAGATCTGAGGTTGAAATGGAAGAAATTTAAGCACTTGGCCGGTCAAGTTGGTGGAGCAAGAAATATATTTTGTGAGCTGTTTGGACAAGATAAAGCTGAAAACACCTTTTGGTTAGACAGTTCATCAATTGAGAAGAGAAGAGCACGTTTTTCATTTATGGGAGGAAAAGGTGGAGCCCTTTGGAAGCAACTGACCTTTAAATTGTCTGATAAAAGTGATATGGCTTTGAAGGGCAGTGGTCTTCTATCTGTTCAGGATGCTCAAGGCTCTATCAAACGTTCATTTTTGGAAGAAGGTTTTCTTGATTTTCTGAAAAAGGAGCTCCTGTCATTTTGTTACGATGAAAAGGATTATGAAGAACTTCCATTTGATTTTCATGGTGGATATATTGGTTACCTCGGGTATAACCTAAAAGTTGAATGTGGTGTGGATTCTAATCGCCACAGATCCGAGACTCCAGATGCTTGCTTTTTCTTTGCTGATAACCTTGTAGTTGTTGATCATCGTAATGATGATGTTTACATACTGTCTGTAGACGAAGTATGCAGACCTTTGACGCCATGGTTGGATGATACAGAACAGAAGCTTCTCAACTTAAAAGCTTCTGCTACAGGGGAGGGAAAGAAGCCAACTTTGCTGGCTCTACAATCTTCACTGTGTCAAGGAAGTTTCCTTGCTGATAAATCAAGAGAAGCATATATAGAAGATGTTGACAAGTGCCTGGAATACATCAAAGATGGTGAAAGTTATGAATTATGTCTGACAACTCAGATGAGAAAAAGAATTGGGGACATAGACTCATTGGGACTTTACCTTCATTTAAGAGAGAAGAATCCAGCACCATATGCTGCCTGGATTAATTTTTCAGATGAAGACCTATGCATCTGCTGTTCTTCCCCTGAGCGTTTCTTACAATTGGATAGAAATGGTGTACTCGAAGCAAAACCCATTAAGGGTACCGTAGCTCGTGGTGCGACACCAGAGGAAGATGAACAACGTAAACTACAACTGCAGTATAGTGAAAAGGATCAAGCTGAAAATCTGATGATAGTTGATCTTCTAAGGAATGACCTTGGTCGTGTGTGTGAGCCTGGCTCAGTTCATGTGCCACGTCTTATGGATGTGGAATCATATGCTACGGTTCATACTATGGTCAGCACAATTCGTGGGCAAAAGCGGTCTGATGTGACTGCAATTGATTGTGTGAAAGCAGCATTTCCGGGTGGTTCGATGACGGGTGCACCGAAATTGAGATCAATGGAACTTCTTGATTCAATCGAAACTAGTTCTCGAGGTATCTACTCGGGCTCCATTGGCTTTTTCTCATATAACCAGACATTTGATCTAAATATAGTTATTAGAACTGTTGTTATACACGAGGGTGAAGCTTCAATAGGAGCGGGGGGAGCCATTGTTGCCCTATCAAATCCTGAAGACGAGTACGAGGAAATGGTTTTGAAAACACGAGCTCCAGCAAAAGCTGTACTAGAATTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

905

Amino Acids

101.27

Weight (kDa)

6.04

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 90 - 251 9.3e-30 Glutamine amidotransferase class-I
GATase PF00117 290 - 327 4.4e-08 Glutamine amidotransferase class-I
Anth_synt_I_N PF04715 437 - 581 2.2e-16 Anthranilate synthase component I, N terminal region
Chorismate_bind PF00425 636 - 893 2.6e-86 chorismate binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 959
Acc65I GGTACC 2 cut(s) 675, 2174
AccB1I GGYRCC 3 cut(s) 235, 675, 2174
AccB7I CCANNNNNTGG 3 cut(s) 587, 685, 830
AccBSI CCGCTC 1 cut(s) 2618
AccI GTMKAC 1 cut(s) 1752
AciI CCGC 3 cut(s) 458, 2395, 2618
AclI AACGTT 1 cut(s) 1495
AclWI GGATC 2 cut(s) 1659, 2253
AcoI YGGCCR 2 cut(s) 646, 1254
AcsI RAATTY 6 cut(s) 254, 794, 976, 1039, 1241, 2708
AcuI CTGAAG 2 cut(s) 461, 2670
AcyI GRCGYC 1 cut(s) 1775
AdeI CACNNNGTG 1 cut(s) 15
AfaI GTAC 9 cut(s) 70, 624, 677, 867, 924, 2151, 2176, 2660, 2703
AfiI CCNNNNNNNGG 9 cut(s) 437, 511, 512, 587, 685, 830, 917, 1076, 2171
AflIII ACRYGT 1 cut(s) 211
AhlI ACTAGT 1 cut(s) 2501
AjiI CACGTC 1 cut(s) 2330
AjnI CCWGG 8 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308
AleI CACNNNNGTG 1 cut(s) 2598
Alw21I GWGCWC 5 cut(s) 612, 1363, 1542, 2461, 2689
Alw26I GTCTC 2 cut(s) 761, 1664
Alw44I GTGCAC 1 cut(s) 2457
AlwI GGATC 2 cut(s) 1659, 2253
AlwNI CAGNNNCTG 2 cut(s) 316, 484
Ama87I CYCGRG 3 cut(s) 1616, 2508, 2521
AoxI GGCC 3 cut(s) 646, 908, 1254
ApaLI GTGCAC 1 cut(s) 2457
ApeKI GCWGC 3 cut(s) 484, 2066, 2429
ApoI RAATTY 6 cut(s) 254, 794, 976, 1039, 1241, 2708
AseI ATTAAT 2 cut(s) 327, 2076
Asp700I GAANNNNTTC 5 cut(s) 720, 838, 879, 1515, 1578
Asp718I GGTACC 2 cut(s) 675, 2174
AspS9I GGNCC 2 cut(s) 434, 735
AsuC2I CCSGG 1 cut(s) 2439
AsuHPI GGTGA 6 cut(s) 115, 181, 420, 834, 1970, 2612
AvaI CYCGRG 3 cut(s) 1616, 2508, 2521
AvaII GGWCC 2 cut(s) 434, 735
BaeGI GKGCMC 1 cut(s) 2461
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 3 cut(s) 235, 675, 2174
BanII GRGCYC 4 cut(s) 1396, 1542, 2528, 2689
BauI CACGAG 3 cut(s) 2184, 2594, 2682
BbsI GAAGAC 4 cut(s) 467, 1451, 2097, 2658
Bbv12I GWGCWC 5 cut(s) 612, 1363, 1542, 2461, 2689
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 471, 2053, 2441
BccI CCATC 4 cut(s) 797, 939, 1124, 1949
BciT130I CCWGG 8 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310
BclI TGATCA 1 cut(s) 1717
BcnI CCSGG 1 cut(s) 2439
BcoDI GTCTC 2 cut(s) 761, 1664
BcuI ACTAGT 1 cut(s) 2501
BfaI CTAG 4 cut(s) 86, 177, 2502, 2705
BfmI CTRYAG 4 cut(s) 726, 1749, 1827, 2228
BfuAI ACCTGC 1 cut(s) 959
BglI GCCNNNNNGGC 1 cut(s) 858
BglII AGATCT 1 cut(s) 1219
BisI GCNGC 3 cut(s) 485, 2067, 2430
BlsI GCNGC 3 cut(s) 486, 2068, 2431
Bme1390I CCNGG 9 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
Bme18I GGWCC 2 cut(s) 434, 735
BmeT110I CYCGRG 3 cut(s) 1616, 2508, 2521
BmgBI CACGTC 1 cut(s) 2330
BmgT120I GGNCC 2 cut(s) 434, 735
BmiI GGNNCC 6 cut(s) 237, 677, 1393, 2176, 2527, 2626
BmrFI CCNGG 9 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
BoxI GACNNNNGTC 1 cut(s) 2292
BpiI GAAGAC 4 cut(s) 467, 1451, 2097, 2658
BplI GAGNNNNNCTC 2 cut(s) 2297, 2329
BpmI CTGGAG 3 cut(s) 251, 1659, 2673
Bpu10I CCTNAGC 2 cut(s) 192, 2118
BpuEI CTTGAG 3 cut(s) 123, 616, 1464
BpuMI CCSGG 1 cut(s) 2439
BsaAI YACGTR 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 1653
BsaHI GRCGYC 1 cut(s) 1775
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 6 cut(s) 523, 586, 955, 1615, 1778, 2290
BsaXI ACNNNNNCTCC 8 cut(s) 1529, 1559, 2510, 2540, 2616, 2646, 2671, 2701
Bsc4I CCNNNNNNNGG 9 cut(s) 437, 511, 512, 587, 685, 830, 917, 1076, 2171
Bse118I RCCGGY 1 cut(s) 1256
Bse1I ACTGG 3 cut(s) 234, 581, 690
Bse8I GATNNNNATC 1 cut(s) 1653
BseBI CCWGG 8 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310
BseDI CCNNGG 6 cut(s) 523, 586, 955, 1615, 1778, 2290
BseGI GGATG 5 cut(s) 931, 1198, 1480, 1792, 2344
BseJI GATNNNNATC 1 cut(s) 1653
BseLI CCNNNNNNNGG 9 cut(s) 437, 511, 512, 587, 685, 830, 917, 1076, 2171
BseMII CTCAG 7 cut(s) 21, 206, 998, 1214, 2000, 2109, 2328
BseNI ACTGG 3 cut(s) 234, 581, 690
BseSI GKGCMC 1 cut(s) 2461
BseXI GCAGC 3 cut(s) 471, 2053, 2441
BseYI CCCAGC 1 cut(s) 454
BshFI GGCC 3 cut(s) 648, 910, 1256
BshNI GGYRCC 3 cut(s) 235, 675, 2174
BsiHKAI GWGCWC 5 cut(s) 612, 1363, 1542, 2461, 2689
BsiHKCI CYCGRG 3 cut(s) 1616, 2508, 2521
BsiSI CCGG 2 cut(s) 1257, 2438
BslFI GGGAC 3 cut(s) 488, 2021, 2037
BslI CCNNNNNNNGG 9 cut(s) 437, 511, 512, 587, 685, 830, 917, 1076, 2171
BsmAI GTCTC 2 cut(s) 761, 1664
BsmFI GGGAC 3 cut(s) 488, 2021, 2037
BsmI GAATGC 2 cut(s) 754, 898
BsnI GGCC 3 cut(s) 648, 910, 1256
Bso31I GGTCTC 1 cut(s) 761
BsoBI CYCGRG 3 cut(s) 1616, 2508, 2521
Bsp1286I GDGCHC 7 cut(s) 612, 1363, 1396, 1542, 2461, 2528, 2689
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
Bsp19I CCATGG 3 cut(s) 586, 955, 1778
BspACI CCGC 3 cut(s) 458, 2395, 2618
BspANI GGCC 3 cut(s) 648, 910, 1256
BspCNI CTCAG 7 cut(s) 22, 205, 999, 1215, 1999, 2110, 2327
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 6 cut(s) 237, 677, 1393, 2176, 2527, 2626
BspMAI CTGCAG 1 cut(s) 2232
BspMI ACCTGC 1 cut(s) 959
BspPI GGATC 2 cut(s) 1659, 2253
BspQI GCTCTTC 1 cut(s) 1351
BspT107I GGYRCC 3 cut(s) 235, 675, 2174
BspTNI GGTCTC 1 cut(s) 761
BsrBI CCGCTC 1 cut(s) 2618
BsrFI RCCGGY 1 cut(s) 1256
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 3 cut(s) 234, 581, 690
BssAI RCCGGY 1 cut(s) 1256
BssECI CCNNGG 6 cut(s) 523, 586, 955, 1615, 1778, 2290
BssMI GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
BssNI GRCGYC 1 cut(s) 1775
BssSI CACGAG 3 cut(s) 2184, 2594, 2682
BssT1I CCWWGG 5 cut(s) 523, 586, 955, 1778, 2290
Bst2BI CACGAG 3 cut(s) 2184, 2594, 2682
Bst2UI CCWGG 8 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310
Bst4CI ACNGT 8 cut(s) 99, 927, 1337, 1746, 1875, 2179, 2359, 2584
Bst6I CTCTTC 3 cut(s) 745, 1346, 1351
BstACI GRCGYC 1 cut(s) 1775
BstAPI GCANNNNNTGC 1 cut(s) 2063
BstAUI TGTACA 1 cut(s) 922
BstBAI YACGTR 1 cut(s) 547
BstC8I GCNNGC 2 cut(s) 1684, 1857
BstDEI CTNAG 9 cut(s) 30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
BstDSI CCRYGG 3 cut(s) 586, 955, 1778
BstEII GGTNACC 1 cut(s) 1610
BstF5I GGATG 5 cut(s) 931, 1198, 1480, 1792, 2344
BstKTI GATC 8 cut(s) 1013, 1222, 1667, 1720, 2248, 2275, 2474, 2563
BstMAI GTCTC 2 cut(s) 761, 1664
BstMBI GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
BstMWI GCNNNNNNNGC 7 cut(s) 858, 1852, 2063, 2189, 2532, 2624, 2633
BstNI CCWGG 8 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310
BstNSI RCATGY 3 cut(s) 215, 453, 1063
BstPAI GACNNNNGTC 1 cut(s) 2292
BstPI GGTNACC 1 cut(s) 1610
BstSCI CCNGG 9 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308, 2437
BstSFI CTRYAG 4 cut(s) 726, 1749, 1827, 2228
BstSLI GKGCMC 1 cut(s) 2461
BstV1I GCAGC 3 cut(s) 471, 2053, 2441
BstV2I GAAGAC 4 cut(s) 467, 1451, 2097, 2658
BstX2I RGATCY 2 cut(s) 1219, 1664
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 2 cut(s) 1219, 1664
BsuRI GGCC 3 cut(s) 648, 910, 1256
BtgI CCRYGG 3 cut(s) 586, 955, 1778
BtrI CACGTC 1 cut(s) 2330
BtsCI GGATG 5 cut(s) 931, 1198, 1480, 1792, 2344
BtsI GCAGTG 1 cut(s) 1459
BtsIMutI CAGTG 3 cut(s) 683, 1459, 1871
BveI ACCTGC 1 cut(s) 959
Cac8I GCNNGC 2 cut(s) 1684, 1857
CaiI CAGNNNCTG 2 cut(s) 316, 484
CciI TCATGA 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 1256
Cfr13I GGNCC 2 cut(s) 434, 735
CseI GACGC 2 cut(s) 600, 1783
CsiI ACCWGGT 2 cut(s) 238, 436
Csp6I GTAC 9 cut(s) 69, 623, 676, 866, 923, 2150, 2175, 2659, 2702
CviQI GTAC 9 cut(s) 69, 623, 676, 866, 923, 2150, 2175, 2659, 2702
DdeI CTNAG 9 cut(s) 30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
DpnI GATC 8 cut(s) 1012, 1221, 1666, 1719, 2247, 2274, 2473, 2562
DpnII GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
DraI TTTAAA 1 cut(s) 1417
DraIII CACNNNGTG 1 cut(s) 15
EaeI YGGCCR 2 cut(s) 646, 1254
Eam1104I CTCTTC 3 cut(s) 745, 1346, 1351
EarI CTCTTC 3 cut(s) 745, 1346, 1351
Ecl136II GAGCTC 2 cut(s) 1540, 2687
Eco130I CCWWGG 5 cut(s) 523, 586, 955, 1778, 2290
Eco147I AGGCCT 1 cut(s) 910
Eco24I GRGCYC 4 cut(s) 1396, 1542, 2528, 2689
Eco31I GGTCTC 1 cut(s) 761
Eco32I GATATC 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 434, 735
Eco53kI GAGCTC 2 cut(s) 1540, 2687
Eco57I CTGAAG 2 cut(s) 461, 2670
Eco88I CYCGRG 3 cut(s) 1616, 2508, 2521
Eco91I GGTNACC 1 cut(s) 1610
EcoICRI GAGCTC 2 cut(s) 1540, 2687
EcoO65I GGTNACC 1 cut(s) 1610
EcoRII CCWGG 8 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308
EcoRV GATATC 1 cut(s) 41
EcoT14I CCWWGG 5 cut(s) 523, 586, 955, 1778, 2290
EcoT22I ATGCAT 3 cut(s) 409, 900, 2102
EcoT38I GRGCYC 4 cut(s) 1396, 1542, 2528, 2689
ErhI CCWWGG 5 cut(s) 523, 586, 955, 1778, 2290
FalI AAGNNNNNCTT 3 cut(s) 28, 1503, 1535
FaqI GGGAC 3 cut(s) 488, 2021, 2037
FauI CCCGC 1 cut(s) 2611
FauNDI CATATG 2 cut(s) 2062, 2350
FbaI TGATCA 1 cut(s) 1717
FblI GTMKAC 1 cut(s) 1752
Fnu4HI GCNGC 3 cut(s) 485, 2067, 2430
FokI GGATG 5 cut(s) 918, 1185, 1487, 1799, 2351
FriOI GRGCYC 4 cut(s) 1396, 1542, 2528, 2689
Fsp4HI GCNGC 3 cut(s) 485, 2067, 2430
FspBI CTAG 4 cut(s) 86, 177, 2502, 2705
GluI GCNGC 3 cut(s) 485, 2067, 2430
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 3 cut(s) 251, 1659, 2673
HaeIII GGCC 3 cut(s) 648, 910, 1256
HapII CCGG 2 cut(s) 1257, 2438
HgaI GACGC 2 cut(s) 600, 1783
Hin1I GRCGYC 1 cut(s) 1775
HincII GTYRAC 2 cut(s) 1082, 1930
HindII GTYRAC 2 cut(s) 1082, 1930
HindIII AAGCTT 3 cut(s) 1802, 1818, 2604
HpaII CCGG 2 cut(s) 1257, 2438
HphI GGTGA 6 cut(s) 115, 181, 420, 834, 1970, 2612
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 6 cut(s) 100, 855, 954, 1441, 1505, 2042
HpyCH4III ACNGT 8 cut(s) 99, 927, 1337, 1746, 1875, 2179, 2359, 2584
HpyCH4IV ACGT 7 cut(s) 67, 546, 1095, 1363, 1495, 2215, 2329
HpyF10VI GCNNNNNNNGC 7 cut(s) 858, 1852, 2063, 2189, 2532, 2624, 2633
HpyF3I CTNAG 9 cut(s) 30, 35, 192, 1007, 1223, 1986, 2118, 2279, 2314
HpySE526I ACGT 7 cut(s) 67, 546, 1095, 1363, 1495, 2215, 2329
Hsp92I GRCGYC 1 cut(s) 1775
KpnI GGTACC 2 cut(s) 679, 2178
Ksp22I TGATCA 1 cut(s) 1717
Kzo9I GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
LguI GCTCTTC 1 cut(s) 1351
LmnI GCTCC 9 cut(s) 313, 1271, 1391, 1537, 1545, 2531, 2615, 2624, 2692
Lsp1109I GCAGC 3 cut(s) 471, 2053, 2441
MabI ACCWGGT 2 cut(s) 238, 436
MaeI CTAG 4 cut(s) 86, 177, 2502, 2705
MaeII ACGT 7 cut(s) 67, 546, 1095, 1363, 1495, 2215, 2329
MaeIII GTNAC 4 cut(s) 542, 1553, 1610, 2404
MalI GATC 8 cut(s) 1012, 1221, 1666, 1719, 2247, 2274, 2473, 2562
MbiI CCGCTC 1 cut(s) 2618
MboI GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
MfeI CAATTG 3 cut(s) 1344, 2132, 2412
MflI RGATCY 2 cut(s) 1219, 1664
MhlI GDGCHC 7 cut(s) 612, 1363, 1396, 1542, 2461, 2528, 2689
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 1666, 2009
MmeI TCCRAC 3 cut(s) 224, 231, 1764
Mox20I TGGCCA 1 cut(s) 648
Mph1103I ATGCAT 3 cut(s) 409, 900, 2102
MroXI GAANNNNTTC 5 cut(s) 720, 838, 879, 1515, 1578
MscI TGGCCA 1 cut(s) 648
MslI CAYNNNNRTG 4 cut(s) 918, 1064, 1725, 2598
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 2 cut(s) 1257, 2438
MspR9I CCNGG 9 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
MunI CAATTG 3 cut(s) 1344, 2132, 2412
Mva1269I GAATGC 2 cut(s) 754, 898
MvaI CCWGG 8 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310
MwoI GCNNNNNNNGC 7 cut(s) 858, 1852, 2063, 2189, 2532, 2624, 2633
NciI CCSGG 1 cut(s) 2439
NcoI CCATGG 3 cut(s) 586, 955, 1778
NdeI CATATG 2 cut(s) 2062, 2350
NdeII GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
NlaIV GGNNCC 6 cut(s) 237, 677, 1393, 2176, 2527, 2626
NmuCI GTSAC 1 cut(s) 2404
NsiI ATGCAT 3 cut(s) 409, 900, 2102
NspI RCATGY 3 cut(s) 215, 453, 1063
OliI CACNNNNGTG 1 cut(s) 2598
PaeR7I CTCGAG 1 cut(s) 2508
PagI TCATGA 1 cut(s) 151
PceI AGGCCT 1 cut(s) 910
PciI ACATGT 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 1351
PcsI WCGNNNNNNNCGW 1 cut(s) 2459
PctI GAATGC 2 cut(s) 754, 898
PdmI GAANNNNTTC 5 cut(s) 720, 838, 879, 1515, 1578
PfeI GAWTC 8 cut(s) 206, 660, 701, 776, 1649, 2050, 2345, 2489
PflMI CCANNNNNTGG 3 cut(s) 587, 685, 830
PkrI GCNGC 3 cut(s) 486, 2068, 2431
PleI GAGTC 2 cut(s) 1666, 2009
PpsI GAGTC 2 cut(s) 1666, 2009
Ppu21I YACGTR 1 cut(s) 547
PscI ACATGT 1 cut(s) 211
PshAI GACNNNNGTC 1 cut(s) 2292
PshBI ATTAAT 2 cut(s) 327, 2076
Psp124BI GAGCTC 2 cut(s) 1542, 2689
Psp1406I AACGTT 1 cut(s) 1495
Psp6I CCWGG 8 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308
PspEI GGTNACC 1 cut(s) 1610
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 8 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308
PspN4I GGNNCC 6 cut(s) 237, 677, 1393, 2176, 2527, 2626
PspPI GGNCC 2 cut(s) 434, 735
PstI CTGCAG 1 cut(s) 2232
PstNI CAGNNNCTG 2 cut(s) 316, 484
PsuI RGATCY 2 cut(s) 1219, 1664
RsaI GTAC 9 cut(s) 70, 624, 677, 867, 924, 2151, 2176, 2660, 2703
RsaNI GTAC 9 cut(s) 69, 623, 676, 866, 923, 2150, 2175, 2659, 2702
RseI CAYNNNNRTG 4 cut(s) 918, 1064, 1725, 2598
SacI GAGCTC 2 cut(s) 1542, 2689
SapI GCTCTTC 1 cut(s) 1351
SatI GCNGC 3 cut(s) 485, 2067, 2430
Sau3AI GATC 8 cut(s) 1010, 1219, 1664, 1717, 2245, 2272, 2471, 2560
Sau96I GGNCC 2 cut(s) 434, 735
SchI GAGTC 2 cut(s) 1666, 2009
ScrFI CCNGG 9 cut(s) 240, 311, 432, 438, 907, 1940, 2071, 2310, 2439
SduI GDGCHC 7 cut(s) 612, 1363, 1396, 1542, 2461, 2528, 2689
SexAI ACCWGGT 2 cut(s) 238, 436
SfcI CTRYAG 4 cut(s) 726, 1749, 1827, 2228
Sfr274I CTCGAG 1 cut(s) 2508
SinI GGWCC 2 cut(s) 434, 735
SlaI CTCGAG 1 cut(s) 2508
SmiMI CAYNNNNRTG 4 cut(s) 918, 1064, 1725, 2598
SmlI CTYRAG 4 cut(s) 138, 595, 1479, 2508
SmoI CTYRAG 4 cut(s) 138, 595, 1479, 2508
SpeI ACTAGT 1 cut(s) 2501
SseBI AGGCCT 1 cut(s) 910
SsiI CCGC 3 cut(s) 458, 2395, 2618
SspI AATATT 1 cut(s) 969
SspMI CTAG 4 cut(s) 86, 177, 2502, 2705
SstI GAGCTC 2 cut(s) 1542, 2689
StuI AGGCCT 1 cut(s) 910
StyD4I CCNGG 9 cut(s) 238, 309, 430, 436, 905, 1938, 2069, 2308, 2437
StyI CCWWGG 5 cut(s) 523, 586, 955, 1778, 2290
TaaI ACNGT 8 cut(s) 99, 927, 1337, 1746, 1875, 2179, 2359, 2584
TaiI ACGT 7 cut(s) 70, 549, 1098, 1366, 1498, 2218, 2332
TaqI TCGA 4 cut(s) 2154, 2447, 2496, 2509
TatI WGTACW 4 cut(s) 865, 922, 2149, 2701
TfiI GAWTC 8 cut(s) 206, 660, 701, 776, 1649, 2050, 2345, 2489
TscAI CASTG 3 cut(s) 690, 1459, 1878
TseFI GTSAC 1 cut(s) 2404
TseI GCWGC 3 cut(s) 484, 2066, 2429
Tsp45I GTSAC 1 cut(s) 2404
TspGWI ACGGA 1 cut(s) 733
TspRI CASTG 3 cut(s) 690, 1459, 1878
Van91I CCANNNNNTGG 3 cut(s) 587, 685, 830
VneI GTGCAC 1 cut(s) 2457
VpaK11BI GGWCC 2 cut(s) 434, 735
VspI ATTAAT 2 cut(s) 327, 2076
XapI RAATTY 6 cut(s) 254, 794, 976, 1039, 1241, 2708
XceI RCATGY 3 cut(s) 215, 453, 1063
XcmI CCANNNNNNNNNTGG 1 cut(s) 2067
XhoI CTCGAG 1 cut(s) 2508
XmiI GTMKAC 1 cut(s) 1752
XmnI GAANNNNTTC 5 cut(s) 720, 838, 879, 1515, 1578
XspI CTAG 4 cut(s) 86, 177, 2502, 2705
Zsp2I ATGCAT 3 cut(s) 409, 900, 2102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.