Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0008393

Physical Location & Seq

Genomic Coordinates Reverse (-)

18314567 .. 18319433

Gene Structure 4867 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g23510.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGAACATAGTCGATCCGTCAGTGACTCTACTCGCCGCGAGCGGCGGCGACACCGTGAAGCTCTTCGACGTCTCCGTCAAGTCCGGCGATCCATGCGTCTCCAGCTACATTCCTTCCCCCGGCTGCCACGTCAACTCCGTCAAGTGGAACCACACCAATTTGGTTGTGGTGAGCGCCGGAGACGACAAGAAGATATCGCTGTGGCATAAGAACGGGCAGAGCTTGGGGACTATTCCGGTTTCCGGGACCGACAGTGGAGACAACATTGAGGAGTCTATATCGGCGCTTAGCTTCAGTAACAAGGTTTCGAGGTATATATGTTCTGGTGGGAGCGGTCAGGTTGTGAGGATATGGGATTTGCAGAGGAAGAGATGTATTAAATGGTTGAGGGGCCATTCAAGTACGATTACTGGTGCAGCGTACAATTGCAAAGATGAGCACTTGGCTTCCATCAGCCTTAATGGGGATCTCATTATCCACAACTTGGCATCTGGTACGAGAACAACTGAATTCAAGGACCCCAATGGACAGGTACTGAGAGTGCTTGATTATTCCCGGATTAGCCGACACCTGTTGGTGACTGCAGGTGATGATGGTTCTGTGCATCTGTGGGACACAACCGGTCGTAGCCCAAAGATTTCTTGGTTGAAGCAGCATTCTGCACCAACTGCTGGTATCAGTTTCTCACCATCAAATGACAAGGTGTTTGCTAGTGTCGGGCTTGATAAAAAGTTGTATACTTATGACTCTGGGTCTAGAAGACATTCATCTTGCATCTCCTACGAGGCGCCTTTCTCTGCATTGTCATTCAGAGACGATGGCTTGGTACTGGCAGCTGGAACAAGTAGTGGTCGTGTTGTGTTTTATGATGTCCGTGGTAAACCAGAGCCTTTCACTGTTCTTCGTGCTTATAATAGTTCAGAGGCTGTTACAAGTCTATGCTGGCAAAGGTCAAAACCTGTAATTGTAAATGAAAGCAGTTGTTCTGTTGAGACCGCGCTTTTAGGAGATGCTGTTGAAGATTCAATCTTAATGCCTGATCCACTTCCTTCTGTGACTTCATCAAACCTTTCCATTTCTATGGCGGTATCGACTTCTCGGAATCCAGGCCGTTCAGGATTATCTGCTGATACATTTTCAGTTACAGGAACTGGCAGTGGATTAGGGTCAACCACACTCAATGTATCTAGTGCAGAGGATACACCACATCGCGGCCATTTGTGGCCGGGTGGAACATTGCCAAGATTGCATCCTCCTCTATCTACTTTTAACTTCAAGGATGATATGGAGGTATTTTCCCCTCTTGTGGATGTTCATCCTATCACACCTTCACTTGACAAGCTGTGGGATGACCATAACAGATCAAAGAAGGATAGTGTAGATATAAAGCCATCATCACTATTGTTTCCCTCATCCGGTAGGAGGTTCCCTTTGGCAGATGACGTGTCAAGTGATCATCCGATATTTGATTGGAAGCCTAGCTCAGCCTCTAAACAGGATGATAGCAAGTCTTCGTTTCCTCGGTTAGAATCAACTCCTGCTCTGTATTCTAAGAGTGAAGACTCATCCATCACGCCTCCAGAAGCTTGGGGAGGTGAGAGATTATCTGATAAATATACCAACCTGCTTCAGCCATCAATTAAGCCGTCTCGCTTTGGGATGTCAGCTCAGACATCAGGATCACTTATGTCTGGATTACCAGATACATCCTTGTCAACAAGTCAGACAAGTATTAGCTCTACAAGCTTAAACTATGCAAATATGCGCATTAAAGATTTGTCTTTCAGTCAGGAGAATTCCTTGGGATTTCCAGAACACGTCAACTCTAGTGCCATCCCTATGTCCCCTGGTAGCAAAGGAATCAGGGGACAGTCTAACCTTGATTCACCAACATCATCTTTGTCCCTCCATCGGAAATTCTCTACTTATGCAGAGAGAATAGGCACTACATCTTCCTTCAGTGACCGAACATCACTGGCAGCGGGTTCACCAAAAACAAAGAAAACAGGAGCTGAAATGAGAGAATTGTTGACTAGCTTGGTGTCTAAGTCAGATACCTCAACGGTCACAGAATCAGGGATTCTTCCAGCTATGAACGGAGGAACCTCACAATTGCCGAAGGCCCCTCAACAAGATGCCCAGCAGGGAAATTCATTTACATATCAGATTTTACAACGTACTCTAGAAGAGACTCTTGATTCCTTTCAGAAGTCAGTACATGAAGATATGAGGAACCTTCATATAGAAATTTTAAGACAATTTCACATGCAAGAGACGGAAATGTCAATGGTAATGAGTAAAATGCTGGCAAACCAAGCTGAGCTAATGGAAGAAGTTAAATCTCTGCGGAAAGAAAACCAGCAACTCCGACAACTGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000242 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005814 GO:0005815 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0005929 GO:0007049 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0009553 GO:0009555 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0022402 GO:0022607 GO:0032465 GO:0032467 GO:0032501 GO:0032502 GO:0032506 GO:0032886 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0036064 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044463 GO:0044464 GO:0045787 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051301 GO:0051302 GO:0051493 GO:0051641 GO:0051726 GO:0051781 GO:0060236 GO:0061640 GO:0065007 GO:0070507 GO:0070727 GO:0071539 GO:0071840 GO:0072698 GO:0090068 GO:0090224 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120025 GO:0120038 GO:1902410 GO:1903047 GO:1905508 GO:2000694

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

794

Amino Acids

85.98

Weight (kDa)

6.61

Isoelectric Point (pI)

50.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	8 - 176	8.8e-18	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	11 - 162	6.1e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	43 - 205	1.3e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	43 - 137	1.4e-09	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	44 - 240	1e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	55 - 246	3.5e-10	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	90 - 249	1.1e-16	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	91 - 245	1.7e-19	WDR5 beta-propeller domain
WD40_Prp19	PF24814	91 - 175	7.2e-07	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	94 - 207	1.9e-07	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	95 - 171	8.1e-07	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	103 - 229	9.7e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	106 - 205	7.6e-07	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	132 - 207	4.6e-06	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	146 - 250	9.7e-06	MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st	PF24817	160 - 319	2.6e-08	WDHD1 first WD40 domain
Beta-prop_EIPR1	PF23609	178 - 245	1e-05	EIPR1 beta-propeller
Beta-prop_WDR5	PF25175	183 - 294	2e-08	WDR5 beta-propeller domain
WD40_Prp19	PF24814	188 - 313	6.7e-13	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	192 - 324	3.6e-12	THOC3 beta-propeller domain
WD40_RFWD3	PF23419	214 - 314	9.7e-06	E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2	PF23414	225 - 313	5.8e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	238 - 319	6.6e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05970 AT5G05970
fragaria_vesca	FvH4_7g23510 FvH4_7g23510 FvH4_7g23510
malus_domestica	MD01G1140700.v1.1 MD07G1205000.v1.1
prunus_persica	Prupe.2G241200_v2.0.a1 Prupe.2G241200_v2.0.a1
pyrus_communis	pycom01g16130 pycom07g19150
rosa_chinensis	RchiOBHm_Chr1g0368661 RchiOBHm_Chr4g0410231
rosa_laevigata	RLG00000027199
rosa_multiflora	Rmu_co8334599.1_g000001 Rmu_sc0001791.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288380
rosa_rugosa	Rorug01G0343400
rosa_samantha	Rh1AG350800 Rh1BG314500 Rh1CG329200 Rh1DG346100
rosa_wichuraiana	Rw0G021660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	912
AarI	CACCTGC	1 cut(s)	575
AasI	GACNNNNNNGTC	2 cut(s)	74, 2281
AatII	GACGTC	1 cut(s)	72
Acc16I	TGCGCA	1 cut(s)	1766
Acc36I	ACCTGC	2 cut(s)	575, 1632
AccB1I	GGYRCC	1 cut(s)	787
AccB7I	CCANNNNNTGG	2 cut(s)	484, 671
AccBSI	CCGCTC	2 cut(s)	42, 333
AccI	GTMKAC	1 cut(s)	737
AccII	CGCG	3 cut(s)	38, 998, 1212
AciI	CCGC	9 cut(s)	36, 42, 45, 333, 996, 1085, 1212, 1982, 2347
AclWI	GGATC	5 cut(s)	8, 83, 474, 1034, 1687
AcoI	YGGCCR	2 cut(s)	1213, 1223
AcsI	RAATTY	5 cut(s)	509, 1795, 1916, 2149, 2247
AcuI	CTGAAG	3 cut(s)	277, 1613, 1942
AcyI	GRCGYC	2 cut(s)	69, 788
AfaI	GTAC	7 cut(s)	403, 422, 496, 534, 828, 2179, 2217
AflIII	ACRYGT	2 cut(s)	1443, 1816
AgeI	ACCGGT	1 cut(s)	620
AgsI	TTSAA	6 cut(s)	399, 514, 649, 1019, 1026, 1276
AhdI	GACNNNNNGTC	1 cut(s)	751
AjiI	CACGTC	3 cut(s)	130, 1444, 1819
AjnI	CCWGG	2 cut(s)	1105, 1846
Alw21I	GWGCWC	1 cut(s)	441
Alw26I	GTCTC	9 cut(s)	76, 103, 174, 252, 807, 986, 1653, 2183, 2267
AlwI	GGATC	5 cut(s)	8, 83, 474, 1034, 1687
AlwNI	CAGNNNCTG	4 cut(s)	535, 926, 1151, 2012
AoxI	GGCC	5 cut(s)	391, 1108, 1213, 1223, 2121
ApeKI	GCWGC	5 cut(s)	123, 416, 652, 833, 1979
ApoI	RAATTY	5 cut(s)	509, 1795, 1916, 2149, 2247
ArsI	GACNNNNNNTTYG	3 cut(s)	689, 721, 2361
AsiGI	ACCGGT	1 cut(s)	620
Asp700I	GAANNNNTTC	2 cut(s)	62, 763
AspLEI	GCGC	5 cut(s)	176, 286, 790, 1000, 1767
AspS9I	GGNCC	4 cut(s)	246, 391, 517, 2122
AsuC2I	CCSGG	4 cut(s)	120, 244, 556, 1227
AsuHPI	GGTGA	7 cut(s)	181, 589, 599, 678, 1607, 1878, 1980
AvaII	GGWCC	2 cut(s)	246, 517
BanI	GGYRCC	1 cut(s)	787
BbsI	GAAGAC	3 cut(s)	766, 1503, 1566
Bbv12I	GWGCWC	1 cut(s)	441
BbvI	GCAGC	5 cut(s)	110, 428, 664, 845, 1991
BccI	CCATC	9 cut(s)	458, 587, 697, 812, 1399, 1577, 1642, 1841, 1917
BceAI	ACGGC	2 cut(s)	1095, 1630
BciT130I	CCWGG	2 cut(s)	1107, 1848
BciVI	GTATCC	1 cut(s)	1192
BclI	TGATCA	1 cut(s)	1453
BcnI	CCSGG	4 cut(s)	120, 244, 556, 1227
BcoDI	GTCTC	9 cut(s)	76, 103, 174, 252, 807, 986, 1653, 2183, 2267
BfaI	CTAG	7 cut(s)	711, 756, 1188, 1479, 1827, 2034, 2183
BfmI	CTRYAG	1 cut(s)	582
BfoI	RGCGCY	3 cut(s)	177, 287, 791
BfuAI	ACCTGC	2 cut(s)	575, 1632
BfuI	GTATCC	1 cut(s)	1192
BisI	GCNGC	9 cut(s)	36, 43, 46, 124, 417, 653, 834, 1213, 1980
BlpI	GCTNAGC	3 cut(s)	287, 1483, 2319
BlsI	GCNGC	9 cut(s)	37, 44, 47, 125, 418, 654, 835, 1214, 1981
Bme1390I	CCNGG	6 cut(s)	120, 244, 556, 1107, 1227, 1848
Bme18I	GGWCC	2 cut(s)	246, 517
BmeRI	GACNNNNNGTC	1 cut(s)	751
BmgBI	CACGTC	3 cut(s)	130, 1444, 1819
BmgT120I	GGNCC	4 cut(s)	246, 391, 517, 2122
BmiI	GGNNCC	9 cut(s)	149, 247, 392, 519, 789, 1427, 2104, 2124, 2234
BmrFI	CCNGG	6 cut(s)	120, 244, 556, 1107, 1227, 1848
BmsI	GCATC	6 cut(s)	497, 613, 783, 1000, 1258, 2125
BpiI	GAAGAC	3 cut(s)	766, 1503, 1566
BpmI	CTGGAG	2 cut(s)	85, 1563
Bpu1102I	GCTNAGC	3 cut(s)	287, 1483, 2319
BpuMI	CCSGG	4 cut(s)	120, 244, 556, 1227
BsaBI	GATNNNNATC	1 cut(s)	1314
BsaHI	GRCGYC	2 cut(s)	69, 788
BsaI	GGTCTC	1 cut(s)	986
BsaJI	CCNNGG	5 cut(s)	118, 874, 1520, 1800, 1846
BsaWI	WCCGGW	3 cut(s)	235, 620, 1415
BsaXI	ACNNNNNCTCC	4 cut(s)	119, 149, 999, 1029
Bse118I	RCCGGY	1 cut(s)	620
Bse1I	ACTGG	4 cut(s)	415, 834, 1156, 1980
Bse3DI	GCAATG	1 cut(s)	1235
Bse8I	GATNNNNATC	1 cut(s)	1314
BseBI	CCWGG	2 cut(s)	1107, 1848
BseDI	CCNNGG	5 cut(s)	118, 874, 1520, 1800, 1846
BseJI	GATNNNNATC	1 cut(s)	1314
BseMI	GCAATG	1 cut(s)	1235
BseMII	CTCAG	4 cut(s)	527, 1497, 1682, 2310
BseNI	ACTGG	4 cut(s)	415, 834, 1156, 1980
BseRI	GAGGAG	2 cut(s)	284, 1245
BseXI	GCAGC	5 cut(s)	110, 428, 664, 845, 1991
BseYI	CCCAGC	1 cut(s)	2139
BsgI	GTGCAG	3 cut(s)	435, 645, 1212
Bsh1236I	CGCG	3 cut(s)	38, 998, 1212
Bsh1285I	CGRYCG	1 cut(s)	625
BshFI	GGCC	5 cut(s)	393, 1110, 1215, 1225, 2123
BshNI	GGYRCC	1 cut(s)	787
BshTI	ACCGGT	1 cut(s)	620
BsiEI	CGRYCG	1 cut(s)	625
BsiHKAI	GWGCWC	1 cut(s)	441
BsiSI	CCGG	9 cut(s)	84, 120, 177, 236, 243, 556, 621, 1226, 1416
BslFI	GGGAC	6 cut(s)	241, 259, 626, 1828, 1881, 1888
BsmAI	GTCTC	9 cut(s)	76, 103, 174, 252, 807, 986, 1653, 2183, 2267
BsmBI	CGTCTC	6 cut(s)	76, 103, 174, 807, 1653, 2267
BsmFI	GGGAC	6 cut(s)	241, 259, 626, 1828, 1881, 1888
BsmI	GAATGC	1 cut(s)	655
BsnI	GGCC	5 cut(s)	393, 1110, 1215, 1225, 2123
Bso31I	GGTCTC	1 cut(s)	986
Bsp1286I	GDGCHC	1 cut(s)	441
Bsp143I	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
Bsp1720I	GCTNAGC	3 cut(s)	287, 1483, 2319
BspACI	CCGC	9 cut(s)	36, 42, 45, 333, 996, 1085, 1212, 1982, 2347
BspANI	GGCC	5 cut(s)	393, 1110, 1215, 1225, 2123
BspCNI	CTCAG	4 cut(s)	528, 1496, 1681, 2311
BspFNI	CGCG	3 cut(s)	38, 998, 1212
BspLI	GGNNCC	9 cut(s)	149, 247, 392, 519, 789, 1427, 2104, 2124, 2234
BspMAI	CTGCAG	1 cut(s)	586
BspMI	ACCTGC	2 cut(s)	575, 1632
BspPI	GGATC	5 cut(s)	8, 83, 474, 1034, 1687
BspQI	GCTCTTC	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	787
BspTNI	GGTCTC	1 cut(s)	986
BsrBI	CCGCTC	2 cut(s)	42, 333
BsrDI	GCAATG	1 cut(s)	1235
BsrFI	RCCGGY	1 cut(s)	620
BsrI	ACTGG	4 cut(s)	415, 834, 1156, 1980
BssAI	RCCGGY	1 cut(s)	620
BssECI	CCNNGG	5 cut(s)	118, 874, 1520, 1800, 1846
BssMI	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
BssNAI	GTATAC	1 cut(s)	738
BssNI	GRCGYC	2 cut(s)	69, 788
BssT1I	CCWWGG	1 cut(s)	1800
Bst1107I	GTATAC	1 cut(s)	738
Bst2UI	CCWGG	2 cut(s)	1107, 1848
Bst4CI	ACNGT	5 cut(s)	55, 254, 898, 1872, 2065
Bst6I	CTCTTC	3 cut(s)	68, 362, 2181
BstACI	GRCGYC	2 cut(s)	69, 788
BstAPI	GCANNNNNTGC	1 cut(s)	668
BstC8I	GCNNGC	3 cut(s)	40, 944, 2307
BstDEI	CTNAG	7 cut(s)	287, 536, 1483, 1551, 1668, 2046, 2319
BstDSI	CCRYGG	1 cut(s)	874
BstFNI	CGCG	3 cut(s)	38, 998, 1212
BstH2I	RGCGCY	3 cut(s)	177, 287, 791
BstHHI	GCGC	5 cut(s)	176, 286, 790, 1000, 1767
BstKTI	GATC	7 cut(s)	16, 91, 469, 1042, 1364, 1456, 1682
BstMAI	GTCTC	9 cut(s)	76, 103, 174, 252, 807, 986, 1653, 2183, 2267
BstMBI	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
BstMCI	CGRYCG	1 cut(s)	625
BstMWI	GCNNNNNNNGC	6 cut(s)	93, 102, 668, 1246, 1743, 2315
BstNI	CCWGG	2 cut(s)	1107, 1848
BstNSI	RCATGY	1 cut(s)	2269
BstSCI	CCNGG	6 cut(s)	118, 242, 554, 1105, 1225, 1846
BstSFI	CTRYAG	1 cut(s)	582
BstUI	CGCG	3 cut(s)	38, 998, 1212
BstV1I	GCAGC	5 cut(s)	110, 428, 664, 845, 1991
BstV2I	GAAGAC	3 cut(s)	766, 1503, 1566
BstX2I	RGATCY	1 cut(s)	466
BstXI	CCANNNNNNTGG	2 cut(s)	1081, 1587
BstYI	RGATCY	1 cut(s)	466
BstZ17I	GTATAC	1 cut(s)	738
BsuI	GTATCC	1 cut(s)	1192
BsuRI	GGCC	5 cut(s)	393, 1110, 1215, 1225, 2123
BtgI	CCRYGG	1 cut(s)	874
BtgZI	GCGATG	1 cut(s)	1193
BtrI	CACGTC	3 cut(s)	130, 1444, 1819
BtsI	GCAGTG	1 cut(s)	1162
BtsIMutI	CAGTG	6 cut(s)	27, 259, 894, 1162, 1966, 1973
BveI	ACCTGC	2 cut(s)	575, 1632
Cac8I	GCNNGC	3 cut(s)	40, 944, 2307
CaiI	CAGNNNCTG	4 cut(s)	535, 926, 1151, 2012
CfoI	GCGC	5 cut(s)	176, 286, 790, 1000, 1767
Cfr10I	RCCGGY	1 cut(s)	620
Cfr13I	GGNCC	4 cut(s)	246, 391, 517, 2122
CseI	GACGC	1 cut(s)	85
Csp6I	GTAC	7 cut(s)	402, 421, 495, 533, 827, 2178, 2216
CspAI	ACCGGT	1 cut(s)	620
CviAII	CATG	3 cut(s)	93, 2219, 2266
CviQI	GTAC	7 cut(s)	402, 421, 495, 533, 827, 2178, 2216
DdeI	CTNAG	7 cut(s)	287, 536, 1483, 1551, 1668, 2046, 2319
DinI	GGCGCC	1 cut(s)	789
DpnI	GATC	7 cut(s)	15, 90, 468, 1041, 1363, 1455, 1681
DpnII	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
DrdI	GACNNNNNNGTC	2 cut(s)	74, 2281
DriI	GACNNNNNGTC	1 cut(s)	751
DseDI	GACNNNNNNGTC	2 cut(s)	74, 2281
EaeI	YGGCCR	2 cut(s)	1213, 1223
Eam1104I	CTCTTC	3 cut(s)	68, 362, 2181
Eam1105I	GACNNNNNGTC	1 cut(s)	751
EarI	CTCTTC	3 cut(s)	68, 362, 2181
Eco130I	CCWWGG	1 cut(s)	1800
Eco31I	GGTCTC	1 cut(s)	986
Eco32I	GATATC	1 cut(s)	195
Eco47I	GGWCC	2 cut(s)	246, 517
Eco57I	CTGAAG	3 cut(s)	277, 1613, 1942
EcoO109I	RGGNCCY	2 cut(s)	517, 2122
EcoRI	GAATTC	2 cut(s)	509, 1795
EcoRII	CCWGG	2 cut(s)	1105, 1846
EcoRV	GATATC	1 cut(s)	195
EcoT14I	CCWWGG	1 cut(s)	1800
EgeI	GGCGCC	1 cut(s)	789
EheI	GGCGCC	1 cut(s)	789
ErhI	CCWWGG	1 cut(s)	1800
Esp3I	CGTCTC	6 cut(s)	76, 103, 174, 807, 1653, 2267
FaeI	CATG	3 cut(s)	96, 2222, 2269
FalI	AAGNNNNNCTT	2 cut(s)	2178, 2210
FaqI	GGGAC	6 cut(s)	241, 259, 626, 1828, 1881, 1888
FatI	CATG	3 cut(s)	92, 2218, 2265
FauI	CCCGC	1 cut(s)	1975
FbaI	TGATCA	1 cut(s)	1453
FblI	GTMKAC	1 cut(s)	737
Fnu4HI	GCNGC	9 cut(s)	36, 43, 46, 124, 417, 653, 834, 1213, 1980
Fsp4HI	GCNGC	9 cut(s)	36, 43, 46, 124, 417, 653, 834, 1213, 1980
FspAI	RTGCGCAY	1 cut(s)	1766
FspBI	CTAG	7 cut(s)	711, 756, 1188, 1479, 1827, 2034, 2183
FspI	TGCGCA	1 cut(s)	1766
GlaI	GCGC	5 cut(s)	175, 285, 789, 999, 1766
GluI	GCNGC	9 cut(s)	36, 43, 46, 124, 417, 653, 834, 1213, 1980
GsaI	CCCAGC	1 cut(s)	2143
GsuI	CTGGAG	2 cut(s)	85, 1563
HaeII	RGCGCY	3 cut(s)	177, 287, 791
HaeIII	GGCC	5 cut(s)	393, 1110, 1215, 1225, 2123
HapII	CCGG	9 cut(s)	84, 120, 177, 236, 243, 556, 621, 1226, 1416
HgaI	GACGC	1 cut(s)	85
HhaI	GCGC	5 cut(s)	176, 286, 790, 1000, 1767
Hin1I	GRCGYC	2 cut(s)	69, 788
Hin1II	CATG	3 cut(s)	96, 2222, 2269
Hin6I	GCGC	5 cut(s)	174, 284, 788, 998, 1765
HinP1I	GCGC	5 cut(s)	174, 284, 788, 998, 1765
HincII	GTYRAC	5 cut(s)	133, 1170, 1716, 1822, 2031
HindII	GTYRAC	5 cut(s)	133, 1170, 1716, 1822, 2031
HindIII	AAGCTT	2 cut(s)	1584, 1744
HpaII	CCGG	9 cut(s)	84, 120, 177, 236, 243, 556, 621, 1226, 1416
HphI	GGTGA	7 cut(s)	181, 589, 599, 678, 1607, 1878, 1980
Hpy166II	GTNNAC	8 cut(s)	133, 738, 881, 1170, 1716, 1822, 1988, 2031
Hpy188III	TCNNGA	9 cut(s)	756, 1116, 1580, 1677, 1692, 1790, 1811, 2183, 2195
Hpy8I	GTNNAC	8 cut(s)	133, 738, 881, 1170, 1716, 1822, 1988, 2031
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	7 cut(s)	123, 1059, 1338, 1363, 1966, 2113, 2246
HpyCH4III	ACNGT	5 cut(s)	55, 254, 898, 1872, 2065
HpyCH4IV	ACGT	5 cut(s)	69, 129, 1443, 1818, 2176
HpyF10VI	GCNNNNNNNGC	6 cut(s)	93, 102, 668, 1246, 1743, 2315
HpyF3I	CTNAG	7 cut(s)	287, 536, 1483, 1551, 1668, 2046, 2319
HpySE526I	ACGT	5 cut(s)	69, 129, 1443, 1818, 2176
Hsp92I	GRCGYC	2 cut(s)	69, 788
Hsp92II	CATG	3 cut(s)	96, 2222, 2269
HspAI	GCGC	5 cut(s)	174, 284, 788, 998, 1765
KasI	GGCGCC	1 cut(s)	787
Ksp22I	TGATCA	1 cut(s)	1453
Kzo9I	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
LguI	GCTCTTC	1 cut(s)	68
LmnI	GCTCC	2 cut(s)	330, 2009
Lsp1109I	GCAGC	5 cut(s)	110, 428, 664, 845, 1991
LweI	GCATC	6 cut(s)	497, 613, 783, 1000, 1258, 2125
MaeI	CTAG	7 cut(s)	711, 756, 1188, 1479, 1827, 2034, 2183
MaeII	ACGT	5 cut(s)	69, 129, 1443, 1818, 2176
MaeIII	GTNAC	8 cut(s)	22, 296, 577, 928, 1054, 1141, 1961, 2065
MalI	GATC	7 cut(s)	15, 90, 468, 1041, 1363, 1455, 1681
MbiI	CCGCTC	2 cut(s)	42, 333
MboI	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
MfeI	CAATTG	2 cut(s)	424, 2111
MflI	RGATCY	1 cut(s)	466
MhlI	GDGCHC	1 cut(s)	441
Mly113I	GGCGCC	1 cut(s)	788
MlyI	GAGTC	5 cut(s)	19, 281, 740, 1556, 2185
MroXI	GAANNNNTTC	2 cut(s)	62, 763
MseI	TTAA	9 cut(s)	378, 459, 1031, 1269, 1641, 1748, 1770, 2252, 2337
MslI	CAYNNNNRTG	2 cut(s)	1079, 1838
MspA1I	CMGCKG	2 cut(s)	836, 1982
MspI	CCGG	9 cut(s)	84, 120, 177, 236, 243, 556, 621, 1226, 1416
MspR9I	CCNGG	6 cut(s)	120, 244, 556, 1107, 1227, 1848
MunI	CAATTG	2 cut(s)	424, 2111
Mva1269I	GAATGC	1 cut(s)	655
MvaI	CCWGG	2 cut(s)	1107, 1848
MvnI	CGCG	3 cut(s)	38, 998, 1212
MwoI	GCNNNNNNNGC	6 cut(s)	93, 102, 668, 1246, 1743, 2315
NarI	GGCGCC	1 cut(s)	788
NciI	CCSGG	4 cut(s)	120, 244, 556, 1227
NdeII	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
NlaIII	CATG	3 cut(s)	96, 2222, 2269
NlaIV	GGNNCC	9 cut(s)	149, 247, 392, 519, 789, 1427, 2104, 2124, 2234
NmuCI	GTSAC	5 cut(s)	22, 577, 1054, 1961, 2065
NsbI	TGCGCA	1 cut(s)	1766
NspI	RCATGY	1 cut(s)	2269
PaqCI	CACCTGC	1 cut(s)	575
PciSI	GCTCTTC	1 cut(s)	68
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 135
PctI	GAATGC	1 cut(s)	655
PdmI	GAANNNNTTC	2 cut(s)	62, 763
PfeI	GAWTC	8 cut(s)	1022, 1102, 1529, 1860, 1883, 2072, 2080, 2198
PflMI	CCANNNNNTGG	2 cut(s)	484, 671
PfoI	TCCNGGA	2 cut(s)	242, 554
PinAI	ACCGGT	1 cut(s)	620
PkrI	GCNGC	9 cut(s)	37, 44, 47, 125, 418, 654, 835, 1214, 1981
PleI	GAGTC	5 cut(s)	19, 280, 740, 1556, 2185
PluTI	GGCGCC	1 cut(s)	791
PpsI	GAGTC	5 cut(s)	19, 280, 740, 1556, 2185
PpuMI	RGGWCCY	1 cut(s)	517
PsiI	TTATAA	1 cut(s)	912
Psp5II	RGGWCCY	1 cut(s)	517
Psp6I	CCWGG	2 cut(s)	1105, 1846
PspFI	CCCAGC	1 cut(s)	2139
PspGI	CCWGG	2 cut(s)	1105, 1846
PspN4I	GGNNCC	9 cut(s)	149, 247, 392, 519, 789, 1427, 2104, 2124, 2234
PspPI	GGNCC	4 cut(s)	246, 391, 517, 2122
PspPPI	RGGWCCY	1 cut(s)	517
PstI	CTGCAG	1 cut(s)	586
PstNI	CAGNNNCTG	4 cut(s)	535, 926, 1151, 2012
PsuI	RGATCY	1 cut(s)	466
PvuII	CAGCTG	1 cut(s)	836
RsaI	GTAC	7 cut(s)	403, 422, 496, 534, 828, 2179, 2217
RsaNI	GTAC	7 cut(s)	402, 421, 495, 533, 827, 2178, 2216
RseI	CAYNNNNRTG	2 cut(s)	1079, 1838
SapI	GCTCTTC	1 cut(s)	68
SaqAI	TTAA	9 cut(s)	378, 459, 1031, 1269, 1641, 1748, 1770, 2252, 2337
SatI	GCNGC	9 cut(s)	36, 43, 46, 124, 417, 653, 834, 1213, 1980
Sau3AI	GATC	7 cut(s)	13, 88, 466, 1039, 1361, 1453, 1679
Sau96I	GGNCC	4 cut(s)	246, 391, 517, 2122
SchI	GAGTC	5 cut(s)	19, 281, 740, 1556, 2185
ScrFI	CCNGG	6 cut(s)	120, 244, 556, 1107, 1227, 1848
SduI	GDGCHC	1 cut(s)	441
SfaNI	GCATC	6 cut(s)	497, 613, 783, 1000, 1258, 2125
SfcI	CTRYAG	1 cut(s)	582
SfoI	GGCGCC	1 cut(s)	789
SinI	GGWCC	2 cut(s)	246, 517
SmiMI	CAYNNNNRTG	2 cut(s)	1079, 1838
SsiI	CCGC	9 cut(s)	36, 42, 45, 333, 996, 1085, 1212, 1982, 2347
SspDI	GGCGCC	1 cut(s)	787
SspMI	CTAG	7 cut(s)	711, 756, 1188, 1479, 1827, 2034, 2183
StyD4I	CCNGG	6 cut(s)	118, 242, 554, 1105, 1225, 1846
StyI	CCWWGG	1 cut(s)	1800
TaaI	ACNGT	5 cut(s)	55, 254, 898, 1872, 2065
TaiI	ACGT	5 cut(s)	72, 132, 1446, 1821, 2179
TaqI	TCGA	4 cut(s)	12, 66, 308, 1091
TaqII	GACCGA	2 cut(s)	263, 1980
TatI	WGTACW	1 cut(s)	2215
TauI	GCSGC	4 cut(s)	38, 45, 48, 1215
TfiI	GAWTC	8 cut(s)	1022, 1102, 1529, 1860, 1883, 2072, 2080, 2198
Tru1I	TTAA	9 cut(s)	378, 459, 1031, 1269, 1641, 1748, 1770, 2252, 2337
Tru9I	TTAA	9 cut(s)	378, 459, 1031, 1269, 1641, 1748, 1770, 2252, 2337
TscAI	CASTG	6 cut(s)	27, 259, 901, 1162, 1966, 1980
TseFI	GTSAC	5 cut(s)	22, 577, 1054, 1961, 2065
TseI	GCWGC	5 cut(s)	123, 416, 652, 833, 1979
Tsp45I	GTSAC	5 cut(s)	22, 577, 1054, 1961, 2065
TspDTI	ATGAA	9 cut(s)	17, 756, 987, 1050, 1304, 2108, 2142, 2228, 2235
TspGWI	ACGGA	6 cut(s)	6, 64, 127, 863, 2112, 2291
TspRI	CASTG	6 cut(s)	27, 259, 901, 1162, 1966, 1980
Van91I	CCANNNNNTGG	2 cut(s)	484, 671
VpaK11BI	GGWCC	2 cut(s)	246, 517
XapI	RAATTY	5 cut(s)	509, 1795, 1916, 2149, 2247
XbaI	TCTAGA	2 cut(s)	755, 2182
XceI	RCATGY	1 cut(s)	2269
XcmI	CCANNNNNNNNNTGG	2 cut(s)	163, 639
XmiI	GTMKAC	1 cut(s)	737
XmnI	GAANNNNTTC	2 cut(s)	62, 763
XspI	CTAG	7 cut(s)	711, 756, 1188, 1479, 1827, 2034, 2183
ZraI	GACGTC	1 cut(s)	70

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.