Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008393

Physical Location & Seq

Genomic Coordinates Reverse (-)

28227036 .. 28231984

Gene Structure 4949 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1205000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGAATACTGGGTTGGATCCGTCGATGACGTTGCTGGCAGCAAGCGGCGGCGACACCGTGAAGCTCTTCGATGTATCGGACAAGTCCGCCGATCCCTTGGTTTTGAGCTACACTCCTTCTCCTGGTTCCCATGTCAATTCCCTCAAGTGGAATCACACCAATATGGTGGTGGCTAGTGCTGGAGATGATAAGAAGATATCGTTGTGGAATAAGAATGGGAAGAGCATGGGCACCATTCCGGTGGCCGGGACCGACAGCGGAGACGGCATTGAGGAGTCTATATTGGCTCTCAGCTTCGGTAACAAGGTTTCTAGATACATTTGTTCGGGTGGAACCGGTCAGGTTGTAAGAATATGGGATTTGCAGAGAAAGCGGTGTATCAAATGGTTGAGGGGTCACTCTAGTACAATTACGGGTGCAATGTACAATTGCAAAGATGAGCACTTGGCTTCCATCAGTCTTAATGGGAACCTCATACTTCACAACCTTGCATCTGGTACAAGGACTACTGAACTCAAGGACCCCAATCAACAGGTGCTAAGAGTGCTTGACTATTCCCGGATTAGTCGACACCTTCTTGTGACAGCAGGTGATGACGGGTCTGTACATTTGTGGGACACAACCGCTCGTAGCCCAAAGGTTTCTTGGCTGAAGCAGCATTCTGCACCAGCTGCTGGTATCAGTTTCTCTCCATCTAATGACAAGATGATAGCTACCGTTGGGCTTGATAAGAAGTTGTATACTTACGACTCAGGGTCGAGACAACCTTCACATTGTATTTCCTATAAAGCACCTTTCTCTTCATTGGCATTTAGAGATGATGGTTGGGTATTGGCAGCTGGAACAAGCAGTGGTGATGTGGTGTTCTACGATGTCCGTGGTAAACCAGAGCCTTTCACTGGTATTCGTGCTTATAGTAGTTCAGAGGCTGTTACAAGTCTATGCTGGCAAAGGTCAAAACCTGTCATTGTAAACGAAAGCAATTGTACTGTTGAGACTGCTCTTTTAGGAGATGCTGTTGAAGATTCAATCCTTATGCCTGACCAACTTCCTTCTGTGACTTCATCAAGTCTTCCTCCATCCATGGTAGTATCTAGTTCTCGAAATCCAGGCCGTTCAAGTTCATCTGCCGAGACATCAGCAGCTGGCAATGGATTAACATCAAGCACACTTTGTGTATCTACGACAGAGGATACACCACAACGAGACCATTTGTGGCCTGGTAGAACATTGCGTAGGTTACATCCTCCTCGATCTACTTATAACTTCAAGGATGATATGGAGGTGTTCTCCCCTCTTGTGGATGTTCATCCAATCACACCTACACTTGACAAGCTGTGGGATGATGACAAATCAAAGAAGGATAATTTGTTTTCAGATAAGAAACCTTCGTCACTTTTATTTCCTTCGTCCGGTGGGAGATTCCCTTTTGCAGAAGATGGGCCCACTGATCATCCAATATTTGACTGGAAACCCAGTTCAACATCTAAACAGGATGACATAAAATCGACATTTGCACTATTAGAATCAACCCCTGCTCCATCTTCCAAGAGTGAAGACTCATCCATCACCCCTCCAGAAGCTTGGGGTGGTGAGAGATTTTCTGATAAGTTTCGCCAGCCAACCACTTTTCTATCTCGCGTTGGGACATCAGCTCAGACATCAGGATCAACTTTATCTGGACTGCCCGATATGTCTTTAACAGAAAACTTGCAAAGTTTGCGCACCAAAGGTTTGTCTTCCATTCAGGAGACTTCATTTGGATTTCCCGAACACATTTCCTCGAGTGCAATGCCTATGTCCCCTGGTAACAAAGGCCTCTTGGGACAGCCTAACCTTGATGCACCAACATCTTTGACCTTTTCTCGGAGATTCTCTACTTATGCAGAGAGGATAAGCACTACATCTAACTTCAATGATCGAACATCTCTAGCAGTTAGTTCACCGAAAACAAAGAAAACAGGAGCCGAAGGAAGAGAAGAATTGTTGAATAGCTTCTTGTCAAAGTCAGATGCATCAACTGCCACAGAATTAGGGGCTCTTCCAGCAATTAACGGAGGAAGCTTGCATCTCCAAAATGCGCCCCAACAAGATGCACAGCAGGGAAGCTCCTTTACATTTCAGATTTTTCAACGCACTCTAGAAGAAACTCTGGATTCTTTTCAGAAATCCATACACGAAGATATGAGGAACCTTCATATAGAAATTTTAAGGCAATTCCATATGCAAGAGATGGAAATGTCAAATGTGATGAGGGCAATGCTGGAAAACCAAGCTGAGCTGATGAAAGAAGTTAAGTCTCTCCGGAAAGAAAACCAGCAGCTCCGGCAATTGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000242 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005814 GO:0005815 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0005929 GO:0007049 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0009553 GO:0009555 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0022402 GO:0022607 GO:0032465 GO:0032467 GO:0032501 GO:0032502 GO:0032506 GO:0032886 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0036064 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044463 GO:0044464 GO:0045787 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051301 GO:0051302 GO:0051493 GO:0051641 GO:0051726 GO:0051781 GO:0060236 GO:0061640 GO:0065007 GO:0070507 GO:0070727 GO:0071539 GO:0071840 GO:0072698 GO:0090068 GO:0090224 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120025 GO:0120038 GO:1902410 GO:1903047 GO:1905508 GO:2000694

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

780

Amino Acids

84.95

Weight (kDa)

6.34

Isoelectric Point (pI)

51.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	8 - 194	2.2e-21	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	10 - 128	8.3e-06	WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	12 - 163	8.8e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	44 - 138	3.1e-10	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	59 - 246	7.1e-09	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	91 - 177	8.3e-07	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	92 - 241	1.3e-13	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	103 - 250	1.8e-16	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	104 - 210	6e-07	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	105 - 232	1.6e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	108 - 206	6.9e-06	TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	147 - 250	8.6e-06	MABP1/WDR62 second WD40 domain
WDR55	PF24796	147 - 292	1.6e-06	WDR55
Beta-prop_RIG_2nd	PF23775	168 - 241	4.6e-06	RIG second beta-propeller
Beta-prop_EIPR1	PF23609	178 - 246	2.1e-06	EIPR1 beta-propeller
Beta-prop_WDR5	PF25175	184 - 293	1.7e-08	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	189 - 320	2e-07	WDHD1 first WD40 domain
WD40_Prp19	PF24814	189 - 296	9.2e-12	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	191 - 325	1.1e-13	THOC3 beta-propeller domain
WD40_RFWD3	PF23419	213 - 315	2.1e-06	E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2	PF23414	226 - 294	3.1e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	238 - 320	4.3e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05970 AT5G05970
fragaria_vesca	FvH4_7g23510 FvH4_7g23510 FvH4_7g23510
malus_domestica	MD01G1140700.v1.1 MD07G1205000.v1.1
prunus_persica	Prupe.2G241200_v2.0.a1 Prupe.2G241200_v2.0.a1
pyrus_communis	pycom01g16130 pycom07g19150
rosa_chinensis	RchiOBHm_Chr1g0368661 RchiOBHm_Chr4g0410231
rosa_laevigata	RLG00000027199
rosa_multiflora	Rmu_co8334599.1_g000001 Rmu_sc0001791.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288380
rosa_rugosa	Rorug01G0343400
rosa_samantha	Rh1AG350800 Rh1BG314500 Rh1CG329200 Rh1DG346100
rosa_wichuraiana	Rw0G021660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1263
AarI	CACCTGC	1 cut(s)	578
Acc16I	TGCGCA	1 cut(s)	1724
Acc36I	ACCTGC	1 cut(s)	578
AccB1I	GGYRCC	1 cut(s)	230
AccB7I	CCANNNNNTGG	1 cut(s)	674
AccBSI	CCGCTC	1 cut(s)	626
AccI	GTMKAC	2 cut(s)	568, 740
AccII	CGCG	1 cut(s)	1641
AccIII	TCCGGA	1 cut(s)	2304
AciI	CCGC	6 cut(s)	45, 48, 87, 258, 373, 624
AclWI	GGATC	4 cut(s)	11, 24, 86, 1675
AcoI	YGGCCR	1 cut(s)	243
AcsI	RAATTY	1 cut(s)	2205
AcuI	CTGAAG	1 cut(s)	671
AdeI	CACNNNGTG	1 cut(s)	1175
AfaI	GTAC	5 cut(s)	406, 425, 499, 606, 988
AfiI	CCNNNNNNNGG	8 cut(s)	122, 147, 245, 674, 899, 1300, 1412, 1866
AgeI	ACCGGT	1 cut(s)	335
AgsI	TTSAA	8 cut(s)	1022, 1029, 1119, 1270, 1482, 1915, 1990, 2132
AhdI	GACNNNNNGTC	1 cut(s)	754
AjnI	CCWGG	4 cut(s)	121, 1108, 1219, 1804
AjuI	GAANNNNNNNTTGG	1 cut(s)	28
AloI	GAACNNNNNNTCC	2 cut(s)	1462, 1494
Alw21I	GWGCWC	1 cut(s)	444
Alw26I	GTCTC	7 cut(s)	255, 754, 989, 1127, 1200, 1745, 2304
AlwI	GGATC	4 cut(s)	11, 24, 86, 1675
AlwNI	CAGNNNCTG	3 cut(s)	674, 929, 1145
Ama87I	CYCGRG	1 cut(s)	1783
Aor13HI	TCCGGA	1 cut(s)	2304
AoxI	GGCC	5 cut(s)	243, 1111, 1217, 1442, 1816
ApaI	GGGCCC	1 cut(s)	1446
ApeKI	GCWGC	6 cut(s)	38, 655, 671, 836, 1142, 2320
ApoI	RAATTY	1 cut(s)	2205
AsiGI	ACCGGT	1 cut(s)	335
Asp700I	GAANNNNTTC	2 cut(s)	65, 1994
AspLEI	GCGC	2 cut(s)	1725, 2083
AspS9I	GGNCC	4 cut(s)	249, 520, 1442, 1443
AsuC2I	CCSGG	2 cut(s)	247, 559
AsuHPI	GGTGA	5 cut(s)	602, 866, 1561, 1604, 1935
AvaI	CYCGRG	1 cut(s)	1783
AvaII	GGWCC	2 cut(s)	249, 520
BaeGI	GKGCMC	2 cut(s)	233, 1446
BaeI	ACNNNNGTAYC	2 cut(s)	307, 340
BamHI	GGATCC	1 cut(s)	16
BanI	GGYRCC	1 cut(s)	230
BanII	GRGCYC	2 cut(s)	1446, 2041
BarI	GAAGNNNNNNTAC	2 cut(s)	2098, 2130
BbsI	GAAGAC	3 cut(s)	1064, 1563, 1731
Bbv12I	GWGCWC	1 cut(s)	444
BbvI	GCAGC	6 cut(s)	50, 658, 667, 848, 1154, 2332
BccI	CCATC	8 cut(s)	461, 700, 815, 1087, 1433, 1549, 1574, 2227
BceAI	ACGGC	2 cut(s)	280, 1098
BcgI	CGANNNNNNTGC	4 cut(s)	13, 47, 1774, 1808
BciT130I	CCWGG	4 cut(s)	123, 1110, 1221, 1806
BciVI	GTATCC	1 cut(s)	1186
BclI	TGATCA	1 cut(s)	1450
BcnI	CCSGG	2 cut(s)	247, 559
BcoDI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1200, 1745, 2304
BfaI	CTAG	6 cut(s)	174, 312, 402, 1095, 1931, 2141
BfuAI	ACCTGC	1 cut(s)	578
BfuI	GTATCC	1 cut(s)	1186
BisI	GCNGC	8 cut(s)	39, 46, 49, 656, 672, 837, 1143, 2321
BlpI	GCTNAGC	1 cut(s)	2277
BlsI	GCNGC	8 cut(s)	40, 47, 50, 657, 673, 838, 1144, 2322
Bme1390I	CCNGG	6 cut(s)	123, 247, 559, 1110, 1221, 1806
Bme18I	GGWCC	2 cut(s)	249, 520
BmeRI	GACNNNNNGTC	1 cut(s)	754
BmeT110I	CYCGRG	1 cut(s)	1783
BmgT120I	GGNCC	4 cut(s)	249, 520, 1442, 1443
BmrFI	CCNGG	6 cut(s)	123, 247, 559, 1110, 1221, 1806
BmrI	ACTGGG	2 cut(s)	18, 1470
BmsI	GCATC	7 cut(s)	500, 1003, 1831, 2002, 2024, 2077, 2083
BmuI	ACTGGG	2 cut(s)	18, 1470
BpiI	GAAGAC	3 cut(s)	1064, 1563, 1731
BplI	GAGNNNNNCTC	4 cut(s)	97, 129, 1274, 1306
BpmI	CTGGAG	2 cut(s)	201, 1560
Bpu1102I	GCTNAGC	1 cut(s)	2277
BpuEI	CTTGAG	2 cut(s)	128, 500
BpuMI	CCSGG	2 cut(s)	247, 559
BsaBI	GATNNNNATC	2 cut(s)	1159, 1308
BsaI	GGTCTC	1 cut(s)	1200
BsaJI	CCNNGG	4 cut(s)	96, 877, 1083, 1804
BsaWI	WCCGGW	4 cut(s)	238, 335, 1412, 2304
BsaXI	ACNNNNNCTCC	6 cut(s)	103, 133, 1002, 1032, 1522, 1552
Bsc4I	CCNNNNNNNGG	8 cut(s)	122, 147, 245, 674, 899, 1300, 1412, 1866
Bse118I	RCCGGY	1 cut(s)	335
Bse1I	ACTGG	4 cut(s)	13, 904, 1472, 1476
Bse3DI	GCAATG	5 cut(s)	426, 1156, 1229, 1797, 2265
Bse8I	GATNNNNATC	2 cut(s)	1159, 1308
BseAI	TCCGGA	1 cut(s)	2304
BseBI	CCWGG	4 cut(s)	123, 1110, 1221, 1806
BseDI	CCNNGG	4 cut(s)	96, 877, 1083, 1804
BseGI	GGATG	9 cut(s)	1079, 1243, 1279, 1309, 1309, 1348, 1453, 1501, 1562
BseJI	GATNNNNATC	2 cut(s)	1159, 1308
BseLI	CCNNNNNNNGG	8 cut(s)	122, 147, 245, 674, 899, 1300, 1412, 1866
BseMI	GCAATG	5 cut(s)	426, 1156, 1229, 1797, 2265
BseMII	CTCAG	4 cut(s)	304, 765, 1670, 2268
BseNI	ACTGG	4 cut(s)	13, 904, 1472, 1476
BseRI	GAGGAG	2 cut(s)	287, 1239
BseSI	GKGCMC	2 cut(s)	233, 1446
BseXI	GCAGC	6 cut(s)	50, 658, 667, 848, 1154, 2332
BsgI	GTGCAG	1 cut(s)	648
Bsh1236I	CGCG	1 cut(s)	1641
BshFI	GGCC	5 cut(s)	245, 1113, 1219, 1444, 1818
BshNI	GGYRCC	1 cut(s)	230
BshTI	ACCGGT	1 cut(s)	335
BsiHKAI	GWGCWC	1 cut(s)	444
BsiHKCI	CYCGRG	1 cut(s)	1783
BsiSI	CCGG	7 cut(s)	239, 246, 336, 559, 1413, 2305, 2326
BslFI	GGGAC	5 cut(s)	262, 629, 1660, 1786, 1839
BslI	CCNNNNNNNGG	8 cut(s)	122, 147, 245, 674, 899, 1300, 1412, 1866
BsmAI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1200, 1745, 2304
BsmBI	CGTCTC	1 cut(s)	255
BsmFI	GGGAC	5 cut(s)	262, 629, 1660, 1786, 1839
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	5 cut(s)	245, 1113, 1219, 1444, 1818
Bso31I	GGTCTC	1 cut(s)	1200
BsoBI	CYCGRG	1 cut(s)	1783
Bsp120I	GGGCCC	1 cut(s)	1442
Bsp1286I	GDGCHC	4 cut(s)	233, 444, 1446, 2041
Bsp13I	TCCGGA	1 cut(s)	2304
Bsp1407I	TGTACA	2 cut(s)	423, 604
Bsp143I	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
Bsp1720I	GCTNAGC	1 cut(s)	2277
Bsp19I	CCATGG	1 cut(s)	1083
BspACI	CCGC	6 cut(s)	45, 48, 87, 258, 373, 624
BspANI	GGCC	5 cut(s)	245, 1113, 1219, 1444, 1818
BspCNI	CTCAG	4 cut(s)	303, 764, 1669, 2269
BspEI	TCCGGA	1 cut(s)	2304
BspFNI	CGCG	1 cut(s)	1641
BspMI	ACCTGC	1 cut(s)	578
BspPI	GGATC	4 cut(s)	11, 24, 86, 1675
BspQI	GCTCTTC	3 cut(s)	71, 215, 2046
BspT107I	GGYRCC	1 cut(s)	230
BspTNI	GGTCTC	1 cut(s)	1200
BsrBI	CCGCTC	1 cut(s)	626
BsrDI	GCAATG	5 cut(s)	426, 1156, 1229, 1797, 2265
BsrFI	RCCGGY	1 cut(s)	335
BsrGI	TGTACA	2 cut(s)	423, 604
BsrI	ACTGG	4 cut(s)	13, 904, 1472, 1476
BssAI	RCCGGY	1 cut(s)	335
BssECI	CCNNGG	4 cut(s)	96, 877, 1083, 1804
BssMI	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
BssNAI	GTATAC	1 cut(s)	741
BssT1I	CCWWGG	2 cut(s)	96, 1083
Bst1107I	GTATAC	1 cut(s)	741
Bst2UI	CCWGG	4 cut(s)	123, 1110, 1221, 1806
Bst4CI	ACNGT	3 cut(s)	58, 718, 991
Bst6I	CTCTTC	5 cut(s)	71, 215, 805, 1969, 2046
BstAPI	GCANNNNNTGC	3 cut(s)	671, 1720, 2021
BstAUI	TGTACA	2 cut(s)	423, 604
BstC8I	GCNNGC	6 cut(s)	36, 43, 947, 1147, 1619, 2066
BstDEI	CTNAG	5 cut(s)	290, 539, 751, 1656, 2277
BstDSI	CCRYGG	2 cut(s)	877, 1083
BstF5I	GGATG	9 cut(s)	1079, 1243, 1279, 1309, 1309, 1348, 1453, 1501, 1562
BstFNI	CGCG	1 cut(s)	1641
BstHHI	GCGC	2 cut(s)	1725, 2083
BstKTI	GATC	6 cut(s)	19, 94, 1256, 1453, 1670, 1921
BstMAI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1200, 1745, 2304
BstMBI	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
BstMWI	GCNNNNNNNGC	9 cut(s)	264, 370, 544, 655, 671, 1720, 2021, 2045, 2326
BstNI	CCWGG	4 cut(s)	123, 1110, 1221, 1806
BstSCI	CCNGG	6 cut(s)	121, 245, 557, 1108, 1219, 1804
BstSLI	GKGCMC	2 cut(s)	233, 1446
BstUI	CGCG	1 cut(s)	1641
BstV1I	GCAGC	6 cut(s)	50, 658, 667, 848, 1154, 2332
BstV2I	GAAGAC	3 cut(s)	1064, 1563, 1731
BstX2I	RGATCY	1 cut(s)	16
BstXI	CCANNNNNNTGG	3 cut(s)	166, 241, 1584
BstYI	RGATCY	1 cut(s)	16
BstZ17I	GTATAC	1 cut(s)	741
BsuI	GTATCC	1 cut(s)	1186
BsuRI	GGCC	5 cut(s)	245, 1113, 1219, 1444, 1818
BtgI	CCRYGG	2 cut(s)	877, 1083
BtsCI	GGATG	9 cut(s)	1079, 1243, 1279, 1309, 1309, 1348, 1453, 1501, 1562
BtsI	GCAGTG	1 cut(s)	856
BtsIMutI	CAGTG	3 cut(s)	856, 897, 1446
BveI	ACCTGC	1 cut(s)	578
Cac8I	GCNNGC	6 cut(s)	36, 43, 947, 1147, 1619, 2066
CaiI	CAGNNNCTG	3 cut(s)	674, 929, 1145
CfoI	GCGC	2 cut(s)	1725, 2083
Cfr10I	RCCGGY	1 cut(s)	335
Cfr13I	GGNCC	4 cut(s)	249, 520, 1442, 1443
Csp6I	GTAC	5 cut(s)	405, 424, 498, 605, 987
CspAI	ACCGGT	1 cut(s)	335
CviAII	CATG	3 cut(s)	131, 226, 1084
CviQI	GTAC	5 cut(s)	405, 424, 498, 605, 987
DdeI	CTNAG	5 cut(s)	290, 539, 751, 1656, 2277
DpnI	GATC	6 cut(s)	18, 93, 1255, 1452, 1669, 1920
DpnII	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
DraIII	CACNNNGTG	1 cut(s)	1175
DriI	GACNNNNNGTC	1 cut(s)	754
EaeI	YGGCCR	1 cut(s)	243
Eam1104I	CTCTTC	5 cut(s)	71, 215, 805, 1969, 2046
Eam1105I	GACNNNNNGTC	1 cut(s)	754
EarI	CTCTTC	5 cut(s)	71, 215, 805, 1969, 2046
EciI	GGCGGA	1 cut(s)	76
Eco130I	CCWWGG	2 cut(s)	96, 1083
Eco147I	AGGCCT	1 cut(s)	1818
Eco24I	GRGCYC	2 cut(s)	1446, 2041
Eco31I	GGTCTC	1 cut(s)	1200
Eco32I	GATATC	1 cut(s)	198
Eco47I	GGWCC	2 cut(s)	249, 520
Eco57I	CTGAAG	1 cut(s)	671
Eco88I	CYCGRG	1 cut(s)	1783
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRII	CCWGG	4 cut(s)	121, 1108, 1219, 1804
EcoRV	GATATC	1 cut(s)	198
EcoT14I	CCWWGG	2 cut(s)	96, 1083
EcoT22I	ATGCAT	1 cut(s)	2017
EcoT38I	GRGCYC	2 cut(s)	1446, 2041
ErhI	CCWWGG	2 cut(s)	96, 1083
Esp3I	CGTCTC	1 cut(s)	255
FaeI	CATG	3 cut(s)	134, 229, 1087
FaqI	GGGAC	5 cut(s)	262, 629, 1660, 1786, 1839
FatI	CATG	3 cut(s)	130, 225, 1083
FauNDI	CATATG	1 cut(s)	2223
FbaI	TGATCA	1 cut(s)	1450
FblI	GTMKAC	2 cut(s)	568, 740
Fnu4HI	GCNGC	8 cut(s)	39, 46, 49, 656, 672, 837, 1143, 2321
FokI	GGATG	9 cut(s)	1066, 1230, 1286, 1296, 1316, 1355, 1440, 1508, 1549
FriOI	GRGCYC	2 cut(s)	1446, 2041
Fsp4HI	GCNGC	8 cut(s)	39, 46, 49, 656, 672, 837, 1143, 2321
FspBI	CTAG	6 cut(s)	174, 312, 402, 1095, 1931, 2141
FspI	TGCGCA	1 cut(s)	1724
GlaI	GCGC	2 cut(s)	1724, 2082
GluI	GCNGC	8 cut(s)	39, 46, 49, 656, 672, 837, 1143, 2321
GsuI	CTGGAG	2 cut(s)	201, 1560
HaeIII	GGCC	5 cut(s)	245, 1113, 1219, 1444, 1818
HapII	CCGG	7 cut(s)	239, 246, 336, 559, 1413, 2305, 2326
HhaI	GCGC	2 cut(s)	1725, 2083
Hin1II	CATG	3 cut(s)	134, 229, 1087
Hin6I	GCGC	2 cut(s)	1723, 2081
HinP1I	GCGC	2 cut(s)	1723, 2081
HincII	GTYRAC	1 cut(s)	569
HindII	GTYRAC	1 cut(s)	569
HindIII	AAGCTT	2 cut(s)	1581, 2062
HinfI	GANTC	9 cut(s)	151, 275, 749, 1025, 1422, 1526, 1559, 1872, 2156
HpaII	CCGG	7 cut(s)	239, 246, 336, 559, 1413, 2305, 2326
HphI	GGTGA	5 cut(s)	602, 866, 1561, 1604, 1935
Hpy166II	GTNNAC	5 cut(s)	569, 741, 884, 973, 1943
Hpy188I	TCNGA	9 cut(s)	79, 925, 1378, 1606, 1659, 1869, 2011, 2124, 2166
Hpy8I	GTNNAC	5 cut(s)	569, 741, 884, 973, 1943
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	9 cut(s)	126, 584, 777, 1062, 1354, 1398, 1416, 1964, 2204
HpyCH4III	ACNGT	3 cut(s)	58, 718, 991
HpyCH4IV	ACGT	1 cut(s)	29
HpyF10VI	GCNNNNNNNGC	9 cut(s)	264, 370, 544, 655, 671, 1720, 2021, 2045, 2326
HpyF3I	CTNAG	5 cut(s)	290, 539, 751, 1656, 2277
HpySE526I	ACGT	1 cut(s)	29
Hsp92II	CATG	3 cut(s)	134, 229, 1087
HspAI	GCGC	2 cut(s)	1723, 2081
Kpn2I	TCCGGA	1 cut(s)	2304
Ksp22I	TGATCA	1 cut(s)	1450
Kzo9I	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
LguI	GCTCTTC	3 cut(s)	71, 215, 2046
LmnI	GCTCC	4 cut(s)	1543, 1964, 2114, 2328
Lsp1109I	GCAGC	6 cut(s)	50, 658, 667, 848, 1154, 2332
LweI	GCATC	7 cut(s)	500, 1003, 1831, 2002, 2024, 2077, 2083
MaeI	CTAG	6 cut(s)	174, 312, 402, 1095, 1931, 2141
MaeII	ACGT	1 cut(s)	29
MaeIII	GTNAC	8 cut(s)	299, 395, 580, 931, 1057, 1239, 1392, 1808
MalI	GATC	6 cut(s)	18, 93, 1255, 1452, 1669, 1920
MbiI	CCGCTC	1 cut(s)	626
MboI	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
MfeI	CAATTG	3 cut(s)	427, 982, 2330
MflI	RGATCY	1 cut(s)	16
MhlI	GDGCHC	4 cut(s)	233, 444, 1446, 2041
MlyI	GAGTC	3 cut(s)	284, 743, 1553
Mph1103I	ATGCAT	1 cut(s)	2017
MroI	TCCGGA	1 cut(s)	2304
MroXI	GAANNNNTTC	2 cut(s)	65, 1994
MseI	TTAA	6 cut(s)	462, 1157, 1700, 2052, 2210, 2295
MslI	CAYNNNNRTG	2 cut(s)	161, 239
MspA1I	CMGCKG	4 cut(s)	258, 671, 839, 1145
MspI	CCGG	7 cut(s)	239, 246, 336, 559, 1413, 2305, 2326
MspR9I	CCNGG	6 cut(s)	123, 247, 559, 1110, 1221, 1806
MunI	CAATTG	3 cut(s)	427, 982, 2330
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	4 cut(s)	123, 1110, 1221, 1806
MvnI	CGCG	1 cut(s)	1641
MwoI	GCNNNNNNNGC	9 cut(s)	264, 370, 544, 655, 671, 1720, 2021, 2045, 2326
NciI	CCSGG	2 cut(s)	247, 559
NcoI	CCATGG	1 cut(s)	1083
NdeI	CATATG	1 cut(s)	2223
NdeII	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
NlaIII	CATG	3 cut(s)	134, 229, 1087
NmeAIII	GCCGAG	1 cut(s)	1156
NmuCI	GTSAC	4 cut(s)	395, 580, 1057, 1392
NsbI	TGCGCA	1 cut(s)	1724
NsiI	ATGCAT	1 cut(s)	2017
PaeR7I	CTCGAG	1 cut(s)	1783
PaqCI	CACCTGC	1 cut(s)	578
PceI	AGGCCT	1 cut(s)	1818
PciSI	GCTCTTC	3 cut(s)	71, 215, 2046
PctI	GAATGC	1 cut(s)	658
PdmI	GAANNNNTTC	2 cut(s)	65, 1994
PfeI	GAWTC	6 cut(s)	151, 1025, 1422, 1526, 1872, 2156
PflMI	CCANNNNNTGG	1 cut(s)	674
PfoI	TCCNGGA	1 cut(s)	557
PinAI	ACCGGT	1 cut(s)	335
PkrI	GCNGC	8 cut(s)	40, 47, 50, 657, 673, 838, 1144, 2322
PleI	GAGTC	3 cut(s)	283, 743, 1553
PpsI	GAGTC	3 cut(s)	283, 743, 1553
PpuMI	RGGWCCY	1 cut(s)	520
PsiI	TTATAA	1 cut(s)	1263
Psp5II	RGGWCCY	1 cut(s)	520
Psp6I	CCWGG	4 cut(s)	121, 1108, 1219, 1804
PspGI	CCWGG	4 cut(s)	121, 1108, 1219, 1804
PspOMI	GGGCCC	1 cut(s)	1442
PspPI	GGNCC	4 cut(s)	249, 520, 1442, 1443
PspPPI	RGGWCCY	1 cut(s)	520
PspXI	VCTCGAGB	1 cut(s)	1783
PstNI	CAGNNNCTG	3 cut(s)	674, 929, 1145
PsuI	RGATCY	1 cut(s)	16
PvuII	CAGCTG	3 cut(s)	671, 839, 1145
RsaI	GTAC	5 cut(s)	406, 425, 499, 606, 988
RsaNI	GTAC	5 cut(s)	405, 424, 498, 605, 987
RseI	CAYNNNNRTG	2 cut(s)	161, 239
SalI	GTCGAC	1 cut(s)	567
SapI	GCTCTTC	3 cut(s)	71, 215, 2046
SaqAI	TTAA	6 cut(s)	462, 1157, 1700, 2052, 2210, 2295
SatI	GCNGC	8 cut(s)	39, 46, 49, 656, 672, 837, 1143, 2321
Sau3AI	GATC	6 cut(s)	16, 91, 1253, 1450, 1667, 1918
Sau96I	GGNCC	4 cut(s)	249, 520, 1442, 1443
SchI	GAGTC	3 cut(s)	284, 743, 1553
ScrFI	CCNGG	6 cut(s)	123, 247, 559, 1110, 1221, 1806
SduI	GDGCHC	4 cut(s)	233, 444, 1446, 2041
SfaNI	GCATC	7 cut(s)	500, 1003, 1831, 2002, 2024, 2077, 2083
Sfr274I	CTCGAG	1 cut(s)	1783
SinI	GGWCC	2 cut(s)	249, 520
SlaI	CTCGAG	1 cut(s)	1783
SmiMI	CAYNNNNRTG	2 cut(s)	161, 239
SmlI	CTYRAG	3 cut(s)	143, 515, 1783
SmoI	CTYRAG	3 cut(s)	143, 515, 1783
SseBI	AGGCCT	1 cut(s)	1818
SsiI	CCGC	6 cut(s)	45, 48, 87, 258, 373, 624
SspI	AATATT	1 cut(s)	1461
SspMI	CTAG	6 cut(s)	174, 312, 402, 1095, 1931, 2141
StuI	AGGCCT	1 cut(s)	1818
StyD4I	CCNGG	6 cut(s)	121, 245, 557, 1108, 1219, 1804
StyI	CCWWGG	2 cut(s)	96, 1083
TaaI	ACNGT	3 cut(s)	58, 718, 991
TaiI	ACGT	1 cut(s)	32
TaqI	TCGA	9 cut(s)	23, 69, 568, 758, 1102, 1252, 1508, 1784, 1921
TaqII	GACCGA	1 cut(s)	266
TatI	WGTACW	4 cut(s)	404, 423, 604, 986
TauI	GCSGC	2 cut(s)	48, 51
TfiI	GAWTC	6 cut(s)	151, 1025, 1422, 1526, 1872, 2156
Tru1I	TTAA	6 cut(s)	462, 1157, 1700, 2052, 2210, 2295
Tru9I	TTAA	6 cut(s)	462, 1157, 1700, 2052, 2210, 2295
TscAI	CASTG	3 cut(s)	856, 904, 1453
TseFI	GTSAC	4 cut(s)	395, 580, 1057, 1392
TseI	GCWGC	6 cut(s)	38, 655, 671, 836, 1142, 2320
Tsp45I	GTSAC	4 cut(s)	395, 580, 1057, 1392
TspDTI	ATGAA	8 cut(s)	17, 792, 1053, 1113, 1298, 1746, 2186, 2300
TspGWI	ACGGA	3 cut(s)	9, 866, 2070
TspRI	CASTG	3 cut(s)	856, 904, 1453
Van91I	CCANNNNNTGG	1 cut(s)	674
VpaK11BI	GGWCC	2 cut(s)	249, 520
XapI	RAATTY	1 cut(s)	2205
XbaI	TCTAGA	2 cut(s)	311, 2140
XcmI	CCANNNNNNNNNTGG	4 cut(s)	166, 642, 1217, 1464
XhoI	CTCGAG	1 cut(s)	1783
XmiI	GTMKAC	2 cut(s)	568, 740
XmnI	GAANNNNTTC	2 cut(s)	65, 1994
XspI	CTAG	6 cut(s)	174, 312, 402, 1095, 1931, 2141
Zsp2I	ATGCAT	1 cut(s)	2017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.