Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0008393

Physical Location & Seq

Genomic Coordinates Forward (+)

9480748 .. 9485588

Gene Structure 4841 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027199

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGAACATAGTGGATCCGTCAGTGACTTTACTGGCCGCAAGCGGCGGCGACACCGTGAAGCTCTTCGACGTTTCCGTCAAGTCCGGCGATCCATGCGTCTCCAGCTACATTCCTTCTCCTGGTTGCCACGTCAACTCCGAGTCTATATCGGCGCTTAGTTTCAGTAACAAGGTTTCGAGATATATATGTTCTGGTGGGAGTGGTCAGGTTGTGAGGATATGGGATTTGCAGAGAAAGAGATGTATTAAATGGTTGAGGGGCCACTCGAGTACGATTACTGGCGCAGCGTACAATTGCAAAGATGAGCACTTGGCTTCCATCAGCCTTAGTGGGGATCTCATAATCCACAACCTGGCATCTAGTACAAGAACAACCGAATTCAAGGACCCTAATGGACAGGTACTAAGAGTGCTTGATTATTCCCGGATTAGCCGACACCTTTTGGTGACTGCAGGTGATGATGGCTCTGTGCATTTGTGGGACACAACTGGTCGTAGCCCAAAGATTTCTTGGTTGAAGCAGCATTCTGCACCAACTGCTGGTATCAGTTTCTCACCGTCAAATGACAAGATGTTTGCTAGTGTCGGGCTCGATAAAAAGTTGTATACTTATGACTCAGGGTCTAGGAGACATTCATCTTGCATCTCCTATGAGGCACCTTTTTCTGCATTGGCATTTAGAGATGATGGCTGGGTACTAGCAGCTGGAACAAGTAGTGGTCGTGTTGTGTTTTATGATGTCCGTGGTAAACCAGAGCCTTTTACTGTTCTTCGTGCTTATAATAGTTCAGAGGCTGTTACAAGTTTATGCTGGCAAAGGTCAAAACCTGTCATTGTAAATGAAAGCAGTTGTTCTGTTGAGACTGCGCTTTTAGGAGATGCTGTTGAAGATTCAATCCTTATGCCTGATCCACTTCCTTCTGTGACTTCATCAAACCTTTCTCTATCTATGGCAGTATCTAGTTCTCGGAATGCAGGCCGTTCTGGTTTATCTGCTGATACATTTTCAGTTACAGCAACTGGCAGTGGATTAGCGTCAACCACACTTAATGTTTCTACTGCAGAGGATACACCACATCGCGGGCATTTGTGGCCAGGTGGAACATTGTCAAGATTGCATGCTCCTCGATCTACTTTTAACTTCAAGGATGATATGGAGGTATTTTCCCCTCTTGTGGATGTTCATCCCATCACACCTACACTTGACAGGCTGTGGGATGATCATGACAGATCAAAGAAGGATAGTGTAGATAAGAAGACTTCATCACTATTATTTCCCTCGTCCAGTAGGAGGTTCCCATTGGCAGAGGATGTGTCAAGTGATCATCCAATATTTGATTGGAAGCCTAGCTCAGCTTCTAAACAGGATGACAGCAAATCTTCGTTTGCTCGATTAGAATCAACTCCTGCTCCGTATTCCAAGAGTGAAGACTCATCCATCACCCCTCCAGAAGCTTGGGGCGGTTTGCGATGTTGTTTTCATAAGCTAAAATCTACTGATGCCGATTGTTTAACTACAAGTAGAGCTGTTAAAATCCGGTTGCTGCTTGTTGAGTGGATGATGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000242 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005814 GO:0005815 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0005929 GO:0007049 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0009553 GO:0009555 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0022402 GO:0022607 GO:0032465 GO:0032467 GO:0032501 GO:0032502 GO:0032506 GO:0032886 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0036064 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044463 GO:0044464 GO:0045787 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051301 GO:0051302 GO:0051493 GO:0051641 GO:0051726 GO:0051781 GO:0060236 GO:0061640 GO:0065007 GO:0070507 GO:0070727 GO:0071539 GO:0071840 GO:0072698 GO:0090068 GO:0090224 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120025 GO:0120038 GO:1902410 GO:1903047 GO:1905508 GO:2000694

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

523

Amino Acids

56.67

Weight (kDa)

7.22

Isoelectric Point (pI)

48.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	10 - 123	8.2e-12	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	11 - 161	6.3e-07	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	41 - 127	9.4e-08	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	43 - 205	5.1e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	44 - 196	2.6e-15	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	46 - 131	1.4e-06	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	50 - 163	1.5e-07	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	51 - 126	2.7e-06	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	59 - 185	1.8e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	88 - 163	4.3e-06	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	102 - 206	2.5e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	104 - 251	7.7e-10	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	115 - 275	3.3e-09	WDHD1 first WD40 domain
Beta-prop_EIPR1	PF23609	133 - 201	1.6e-06	EIPR1 beta-propeller
WD40_Prp19	PF24814	144 - 269	1.3e-12	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	146 - 280	2.6e-13	THOC3 beta-propeller domain
WD40_RFWD3	PF23419	169 - 270	3.9e-06	E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2	PF23414	181 - 269	2.4e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	194 - 275	5.8e-06	CDC20/Fizzy WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05970 AT5G05970
fragaria_vesca	FvH4_7g23510 FvH4_7g23510 FvH4_7g23510
malus_domestica	MD01G1140700.v1.1 MD07G1205000.v1.1
prunus_persica	Prupe.2G241200_v2.0.a1 Prupe.2G241200_v2.0.a1
pyrus_communis	pycom01g16130 pycom07g19150
rosa_chinensis	RchiOBHm_Chr1g0368661 RchiOBHm_Chr4g0410231
rosa_laevigata	RLG00000027199
rosa_multiflora	Rmu_co8334599.1_g000001 Rmu_sc0001791.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288380
rosa_rugosa	Rorug01G0343400
rosa_samantha	Rh1AG350800 Rh1BG314500 Rh1CG329200 Rh1DG346100
rosa_wichuraiana	Rw0G021660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
AarI	CACCTGC	1 cut(s)	443
AasI	GACNNNNNNGTC	1 cut(s)	74
Acc36I	ACCTGC	1 cut(s)	443
AccB1I	GGYRCC	1 cut(s)	655
AccB7I	CCANNNNNTGG	2 cut(s)	352, 539
AccI	GTMKAC	1 cut(s)	605
AccII	CGCG	1 cut(s)	1080
AciI	CCGC	5 cut(s)	36, 42, 45, 1080, 1461
AclWI	GGATC	5 cut(s)	8, 21, 83, 342, 902
AcoI	YGGCCR	2 cut(s)	33, 1091
AcsI	RAATTY	1 cut(s)	377
AfaI	GTAC	5 cut(s)	271, 290, 364, 402, 696
AfiI	CCNNNNNNNGG	7 cut(s)	119, 331, 352, 539, 1079, 1174, 1290
AgsI	TTSAA	5 cut(s)	382, 517, 887, 894, 1144
AhdI	GACNNNNNGTC	1 cut(s)	619
AjiI	CACGTC	1 cut(s)	130
AjnI	CCWGG	3 cut(s)	118, 351, 1093
AluBI	AGCT	8 cut(s)	61, 105, 704, 1350, 1355, 1454, 1486, 1526
AluI	AGCT	8 cut(s)	61, 105, 704, 1350, 1355, 1454, 1486, 1526
Alw21I	GWGCWC	1 cut(s)	309
Alw26I	GTCTC	3 cut(s)	103, 622, 854
AlwI	GGATC	5 cut(s)	8, 21, 83, 342, 902
AlwNI	CAGNNNCTG	2 cut(s)	794, 1019
Ama87I	CYCGRG	1 cut(s)	265
AoxI	GGCC	4 cut(s)	33, 259, 976, 1091
ApeKI	GCWGC	4 cut(s)	284, 520, 701, 1543
ApoI	RAATTY	1 cut(s)	377
ArsI	GACNNNNNNTTYG	2 cut(s)	557, 589
Asp700I	GAANNNNTTC	1 cut(s)	62
AspLEI	GCGC	3 cut(s)	154, 284, 868
AspS9I	GGNCC	2 cut(s)	259, 385
AsuC2I	CCSGG	1 cut(s)	424
AsuHPI	GGTGA	4 cut(s)	457, 467, 546, 1432
AvaI	CYCGRG	1 cut(s)	265
AvaII	GGWCC	1 cut(s)	385
BalI	TGGCCA	1 cut(s)	1093
BamHI	GGATCC	1 cut(s)	13
BanI	GGYRCC	1 cut(s)	655
BanII	GRGCYC	1 cut(s)	591
BbsI	GAAGAC	2 cut(s)	1262, 1434
Bbv12I	GWGCWC	1 cut(s)	309
BbvI	GCAGC	4 cut(s)	296, 532, 713, 1530
BccI	CCATC	5 cut(s)	326, 455, 680, 1196, 1445
BceAI	ACGGC	1 cut(s)	963
BcgI	CGANNNNNNTGC	2 cut(s)	1106, 1140
BciT130I	CCWGG	3 cut(s)	120, 353, 1095
BciVI	GTATCC	1 cut(s)	1060
BclI	TGATCA	2 cut(s)	1219, 1321
BcnI	CCSGG	1 cut(s)	424
BcoDI	GTCTC	3 cut(s)	103, 622, 854
BfaI	CTAG	6 cut(s)	360, 579, 624, 698, 960, 1347
BfmI	CTRYAG	2 cut(s)	450, 1059
BfoI	RGCGCY	1 cut(s)	155
BfuAI	ACCTGC	1 cut(s)	443
BfuI	GTATCC	1 cut(s)	1060
BisI	GCNGC	7 cut(s)	36, 43, 46, 285, 521, 702, 1544
BlpI	GCTNAGC	1 cut(s)	1351
BlsI	GCNGC	7 cut(s)	37, 44, 47, 286, 522, 703, 1545
Bme1390I	CCNGG	4 cut(s)	120, 353, 424, 1095
Bme18I	GGWCC	1 cut(s)	385
BmeRI	GACNNNNNGTC	1 cut(s)	619
BmeT110I	CYCGRG	1 cut(s)	265
BmgBI	CACGTC	1 cut(s)	130
BmgT120I	GGNCC	2 cut(s)	259, 385
BmiI	GGNNCC	5 cut(s)	15, 260, 387, 657, 1295
BmrFI	CCNGG	4 cut(s)	120, 353, 424, 1095
BmsI	GCATC	4 cut(s)	365, 651, 868, 1489
BpiI	GAAGAC	2 cut(s)	1262, 1434
BpmI	CTGGAG	2 cut(s)	85, 1431
Bpu1102I	GCTNAGC	1 cut(s)	1351
BpuMI	CCSGG	1 cut(s)	424
BsaBI	GATNNNNATC	2 cut(s)	1182, 1396
BsaJI	CCNNGG	1 cut(s)	742
BsaWI	WCCGGW	1 cut(s)	1536
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 867, 897, 1393, 1423
Bsc4I	CCNNNNNNNGG	7 cut(s)	119, 331, 352, 539, 1079, 1174, 1290
Bse1I	ACTGG	5 cut(s)	36, 283, 493, 1024, 1284
Bse8I	GATNNNNATC	2 cut(s)	1182, 1396
BseBI	CCWGG	3 cut(s)	120, 353, 1095
BseDI	CCNNGG	1 cut(s)	742
BseGI	GGATG	9 cut(s)	1153, 1183, 1183, 1222, 1315, 1324, 1372, 1433, 1563
BseJI	GATNNNNATC	2 cut(s)	1182, 1396
BseLI	CCNNNNNNNGG	7 cut(s)	119, 331, 352, 539, 1079, 1174, 1290
BseMII	CTCAG	2 cut(s)	630, 1365
BseNI	ACTGG	5 cut(s)	36, 283, 493, 1024, 1284
BseRI	GAGGAG	1 cut(s)	1113
BseXI	GCAGC	4 cut(s)	296, 532, 713, 1530
BseYI	CCCAGC	1 cut(s)	690
BsgI	GTGCAG	1 cut(s)	513
Bsh1236I	CGCG	1 cut(s)	1080
BshFI	GGCC	4 cut(s)	35, 261, 978, 1093
BshNI	GGYRCC	1 cut(s)	655
BsiHKAI	GWGCWC	1 cut(s)	309
BsiHKCI	CYCGRG	1 cut(s)	265
BsiSI	CCGG	3 cut(s)	84, 424, 1537
BslFI	GGGAC	1 cut(s)	494
BslI	CCNNNNNNNGG	7 cut(s)	119, 331, 352, 539, 1079, 1174, 1290
BsmAI	GTCTC	3 cut(s)	103, 622, 854
BsmBI	CGTCTC	1 cut(s)	103
BsmFI	GGGAC	1 cut(s)	494
BsmI	GAATGC	2 cut(s)	523, 976
BsnI	GGCC	4 cut(s)	35, 261, 978, 1093
BsoBI	CYCGRG	1 cut(s)	265
Bsp1286I	GDGCHC	2 cut(s)	309, 591
Bsp143I	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
Bsp1720I	GCTNAGC	1 cut(s)	1351
BspACI	CCGC	5 cut(s)	36, 42, 45, 1080, 1461
BspANI	GGCC	4 cut(s)	35, 261, 978, 1093
BspCNI	CTCAG	2 cut(s)	629, 1364
BspFNI	CGCG	1 cut(s)	1080
BspHI	TCATGA	1 cut(s)	1222
BspLI	GGNNCC	5 cut(s)	15, 260, 387, 657, 1295
BspMAI	CTGCAG	2 cut(s)	454, 1063
BspMI	ACCTGC	1 cut(s)	443
BspPI	GGATC	5 cut(s)	8, 21, 83, 342, 902
BspQI	GCTCTTC	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	655
BsrI	ACTGG	5 cut(s)	36, 283, 493, 1024, 1284
BssECI	CCNNGG	1 cut(s)	742
BssMI	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
BssNAI	GTATAC	1 cut(s)	606
Bst1107I	GTATAC	1 cut(s)	606
Bst2UI	CCWGG	3 cut(s)	120, 353, 1095
Bst4CI	ACNGT	3 cut(s)	55, 558, 766
Bst6I	CTCTTC	1 cut(s)	68
BstAPI	GCANNNNNTGC	1 cut(s)	536
BstC8I	GCNNGC	5 cut(s)	40, 812, 976, 1082, 1119
BstDEI	CTNAG	5 cut(s)	155, 326, 404, 616, 1351
BstDSI	CCRYGG	1 cut(s)	742
BstF5I	GGATG	9 cut(s)	1153, 1183, 1183, 1222, 1315, 1324, 1372, 1433, 1563
BstFNI	CGCG	1 cut(s)	1080
BstH2I	RGCGCY	1 cut(s)	155
BstHHI	GCGC	3 cut(s)	154, 284, 868
BstKTI	GATC	8 cut(s)	16, 91, 337, 910, 1130, 1222, 1232, 1324
BstMAI	GTCTC	3 cut(s)	103, 622, 854
BstMBI	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
BstMWI	GCNNNNNNNGC	4 cut(s)	93, 102, 536, 1090
BstNI	CCWGG	3 cut(s)	120, 353, 1095
BstNSI	RCATGY	1 cut(s)	1121
BstSCI	CCNGG	4 cut(s)	118, 351, 422, 1093
BstSFI	CTRYAG	2 cut(s)	450, 1059
BstUI	CGCG	1 cut(s)	1080
BstV1I	GCAGC	4 cut(s)	296, 532, 713, 1530
BstV2I	GAAGAC	2 cut(s)	1262, 1434
BstX2I	RGATCY	2 cut(s)	13, 334
BstXI	CCANNNNNNTGG	1 cut(s)	1455
BstYI	RGATCY	2 cut(s)	13, 334
BstZ17I	GTATAC	1 cut(s)	606
BsuI	GTATCC	1 cut(s)	1060
BsuRI	GGCC	4 cut(s)	35, 261, 978, 1093
BtgI	CCRYGG	1 cut(s)	742
BtgZI	GCGATG	2 cut(s)	1061, 1483
BtrI	CACGTC	1 cut(s)	130
BtsCI	GGATG	9 cut(s)	1153, 1183, 1183, 1222, 1315, 1324, 1372, 1433, 1563
BtsI	GCAGTG	1 cut(s)	1030
BtsIMutI	CAGTG	2 cut(s)	27, 1030
BveI	ACCTGC	1 cut(s)	443
Cac8I	GCNNGC	5 cut(s)	40, 812, 976, 1082, 1119
CaiI	CAGNNNCTG	2 cut(s)	794, 1019
CciI	TCATGA	1 cut(s)	1222
CfoI	GCGC	3 cut(s)	154, 284, 868
Cfr13I	GGNCC	2 cut(s)	259, 385
CseI	GACGC	2 cut(s)	85, 1023
Csp6I	GTAC	5 cut(s)	270, 289, 363, 401, 695
CviAII	CATG	3 cut(s)	93, 1118, 1223
CviQI	GTAC	5 cut(s)	270, 289, 363, 401, 695
DdeI	CTNAG	5 cut(s)	155, 326, 404, 616, 1351
DpnI	GATC	8 cut(s)	15, 90, 336, 909, 1129, 1221, 1231, 1323
DpnII	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
DrdI	GACNNNNNNGTC	1 cut(s)	74
DriI	GACNNNNNGTC	1 cut(s)	619
DseDI	GACNNNNNNGTC	1 cut(s)	74
EaeI	YGGCCR	2 cut(s)	33, 1091
Eam1104I	CTCTTC	1 cut(s)	68
Eam1105I	GACNNNNNGTC	1 cut(s)	619
EarI	CTCTTC	1 cut(s)	68
Eco24I	GRGCYC	1 cut(s)	591
Eco47I	GGWCC	1 cut(s)	385
Eco88I	CYCGRG	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	385
EcoRI	GAATTC	1 cut(s)	377
EcoRII	CCWGG	3 cut(s)	118, 351, 1093
EcoT38I	GRGCYC	1 cut(s)	591
Esp3I	CGTCTC	1 cut(s)	103
FaeI	CATG	3 cut(s)	96, 1121, 1226
FaqI	GGGAC	1 cut(s)	494
FatI	CATG	3 cut(s)	92, 1117, 1222
FauI	CCCGC	1 cut(s)	1073
FbaI	TGATCA	2 cut(s)	1219, 1321
FblI	GTMKAC	1 cut(s)	605
Fnu4HI	GCNGC	7 cut(s)	36, 43, 46, 285, 521, 702, 1544
FokI	GGATG	8 cut(s)	1160, 1170, 1190, 1229, 1311, 1322, 1379, 1420
FriOI	GRGCYC	1 cut(s)	591
Fsp4HI	GCNGC	7 cut(s)	36, 43, 46, 285, 521, 702, 1544
FspBI	CTAG	6 cut(s)	360, 579, 624, 698, 960, 1347
GlaI	GCGC	3 cut(s)	153, 283, 867
GluI	GCNGC	7 cut(s)	36, 43, 46, 285, 521, 702, 1544
GsaI	CCCAGC	1 cut(s)	694
GsuI	CTGGAG	2 cut(s)	85, 1431
HaeII	RGCGCY	1 cut(s)	155
HaeIII	GGCC	4 cut(s)	35, 261, 978, 1093
HapII	CCGG	3 cut(s)	84, 424, 1537
HgaI	GACGC	2 cut(s)	85, 1023
HhaI	GCGC	3 cut(s)	154, 284, 868
Hin1II	CATG	3 cut(s)	96, 1121, 1226
Hin6I	GCGC	3 cut(s)	152, 282, 866
HinP1I	GCGC	3 cut(s)	152, 282, 866
HincII	GTYRAC	2 cut(s)	133, 1038
HindII	GTYRAC	2 cut(s)	133, 1038
HindIII	AAGCTT	1 cut(s)	1452
HinfI	GANTC	5 cut(s)	140, 614, 890, 1397, 1430
HpaII	CCGG	3 cut(s)	84, 424, 1537
HphI	GGTGA	4 cut(s)	457, 467, 546, 1432
Hpy166II	GTNNAC	4 cut(s)	133, 606, 749, 1038
Hpy188I	TCNGA	3 cut(s)	139, 790, 969
Hpy188III	TCNNGA	4 cut(s)	177, 1110, 1223, 1448
Hpy8I	GTNNAC	4 cut(s)	133, 606, 749, 1038
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	3 cut(s)	123, 927, 1231
HpyCH4III	ACNGT	3 cut(s)	55, 558, 766
HpyCH4IV	ACGT	2 cut(s)	69, 129
HpyF10VI	GCNNNNNNNGC	4 cut(s)	93, 102, 536, 1090
HpyF3I	CTNAG	5 cut(s)	155, 326, 404, 616, 1351
HpySE526I	ACGT	2 cut(s)	69, 129
Hsp92II	CATG	3 cut(s)	96, 1121, 1226
HspAI	GCGC	3 cut(s)	152, 282, 866
Ksp22I	TGATCA	2 cut(s)	1219, 1321
Kzo9I	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
LguI	GCTCTTC	1 cut(s)	68
LmnI	GCTCC	2 cut(s)	1126, 1414
Lsp1109I	GCAGC	4 cut(s)	296, 532, 713, 1530
LweI	GCATC	4 cut(s)	365, 651, 868, 1489
MaeI	CTAG	6 cut(s)	360, 579, 624, 698, 960, 1347
MaeII	ACGT	2 cut(s)	69, 129
MaeIII	GTNAC	6 cut(s)	22, 164, 445, 796, 922, 1009
MalI	GATC	8 cut(s)	15, 90, 336, 909, 1129, 1221, 1231, 1323
MboI	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
MboII	GAAGA	6 cut(s)	55, 761, 899, 1267, 1371, 1439
MfeI	CAATTG	1 cut(s)	292
MflI	RGATCY	2 cut(s)	13, 334
MhlI	GDGCHC	2 cut(s)	309, 591
MlsI	TGGCCA	1 cut(s)	1093
MluCI	AATT	2 cut(s)	292, 377
MluNI	TGGCCA	1 cut(s)	1093
MlyI	GAGTC	3 cut(s)	149, 608, 1424
Mox20I	TGGCCA	1 cut(s)	1093
MroXI	GAANNNNTTC	1 cut(s)	62
MscI	TGGCCA	1 cut(s)	1093
MseI	TTAA	5 cut(s)	246, 1047, 1137, 1511, 1530
Msp20I	TGGCCA	1 cut(s)	1093
MspA1I	CMGCKG	1 cut(s)	704
MspI	CCGG	3 cut(s)	84, 424, 1537
MspR9I	CCNGG	4 cut(s)	120, 353, 424, 1095
MunI	CAATTG	1 cut(s)	292
Mva1269I	GAATGC	2 cut(s)	523, 976
MvaI	CCWGG	3 cut(s)	120, 353, 1095
MvnI	CGCG	1 cut(s)	1080
MwoI	GCNNNNNNNGC	4 cut(s)	93, 102, 536, 1090
NciI	CCSGG	1 cut(s)	424
NdeII	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
NlaIII	CATG	3 cut(s)	96, 1121, 1226
NlaIV	GGNNCC	5 cut(s)	15, 260, 387, 657, 1295
NmuCI	GTSAC	3 cut(s)	22, 445, 922
NspI	RCATGY	1 cut(s)	1121
PaeI	GCATGC	1 cut(s)	1121
PaeR7I	CTCGAG	1 cut(s)	265
PagI	TCATGA	1 cut(s)	1222
PaqCI	CACCTGC	1 cut(s)	443
PciSI	GCTCTTC	1 cut(s)	68
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 135
PctI	GAATGC	2 cut(s)	523, 976
PdmI	GAANNNNTTC	1 cut(s)	62
PfeI	GAWTC	2 cut(s)	890, 1397
PflMI	CCANNNNNTGG	2 cut(s)	352, 539
PfoI	TCCNGGA	1 cut(s)	422
PkrI	GCNGC	7 cut(s)	37, 44, 47, 286, 522, 703, 1545
PleI	GAGTC	3 cut(s)	148, 608, 1424
PpsI	GAGTC	3 cut(s)	148, 608, 1424
PpuMI	RGGWCCY	1 cut(s)	385
PsiI	TTATAA	1 cut(s)	780
Psp5II	RGGWCCY	1 cut(s)	385
Psp6I	CCWGG	3 cut(s)	118, 351, 1093
PspFI	CCCAGC	1 cut(s)	690
PspGI	CCWGG	3 cut(s)	118, 351, 1093
PspN4I	GGNNCC	5 cut(s)	15, 260, 387, 657, 1295
PspPI	GGNCC	2 cut(s)	259, 385
PspPPI	RGGWCCY	1 cut(s)	385
PspXI	VCTCGAGB	1 cut(s)	265
PstI	CTGCAG	2 cut(s)	454, 1063
PstNI	CAGNNNCTG	2 cut(s)	794, 1019
PsuI	RGATCY	2 cut(s)	13, 334
PvuII	CAGCTG	1 cut(s)	704
RsaI	GTAC	5 cut(s)	271, 290, 364, 402, 696
RsaNI	GTAC	5 cut(s)	270, 289, 363, 401, 695
SapI	GCTCTTC	1 cut(s)	68
SaqAI	TTAA	5 cut(s)	246, 1047, 1137, 1511, 1530
SatI	GCNGC	7 cut(s)	36, 43, 46, 285, 521, 702, 1544
Sau3AI	GATC	8 cut(s)	13, 88, 334, 907, 1127, 1219, 1229, 1321
Sau96I	GGNCC	2 cut(s)	259, 385
SchI	GAGTC	3 cut(s)	149, 608, 1424
ScrFI	CCNGG	4 cut(s)	120, 353, 424, 1095
SduI	GDGCHC	2 cut(s)	309, 591
SfaNI	GCATC	4 cut(s)	365, 651, 868, 1489
SfcI	CTRYAG	2 cut(s)	450, 1059
Sfr274I	CTCGAG	1 cut(s)	265
SinI	GGWCC	1 cut(s)	385
SlaI	CTCGAG	1 cut(s)	265
SmlI	CTYRAG	1 cut(s)	265
SmoI	CTYRAG	1 cut(s)	265
SphI	GCATGC	1 cut(s)	1121
Sse9I	AATT	2 cut(s)	292, 377
SsiI	CCGC	5 cut(s)	36, 42, 45, 1080, 1461
SspI	AATATT	1 cut(s)	1332
SspMI	CTAG	6 cut(s)	360, 579, 624, 698, 960, 1347
StyD4I	CCNGG	4 cut(s)	118, 351, 422, 1093
TaaI	ACNGT	3 cut(s)	55, 558, 766
TaiI	ACGT	2 cut(s)	72, 132
TaqI	TCGA	6 cut(s)	66, 176, 266, 591, 1126, 1390
TasI	AATT	2 cut(s)	292, 377
TatI	WGTACW	1 cut(s)	362
TauI	GCSGC	3 cut(s)	38, 45, 48
TfiI	GAWTC	2 cut(s)	890, 1397
Tru1I	TTAA	5 cut(s)	246, 1047, 1137, 1511, 1530
Tru9I	TTAA	5 cut(s)	246, 1047, 1137, 1511, 1530
TscAI	CASTG	2 cut(s)	27, 1030
TseFI	GTSAC	3 cut(s)	22, 445, 922
TseI	GCWGC	4 cut(s)	284, 520, 701, 1543
Tsp45I	GTSAC	3 cut(s)	22, 445, 922
TspDTI	ATGAA	7 cut(s)	17, 624, 855, 918, 1172, 1251, 1469
TspGWI	ACGGA	4 cut(s)	6, 64, 731, 1401
TspRI	CASTG	2 cut(s)	27, 1030
Van91I	CCANNNNNTGG	2 cut(s)	352, 539
VpaK11BI	GGWCC	1 cut(s)	385
XapI	RAATTY	1 cut(s)	377
XceI	RCATGY	1 cut(s)	1121
XcmI	CCANNNNNNNNNTGG	2 cut(s)	507, 1335
XhoI	CTCGAG	1 cut(s)	265
XmiI	GTMKAC	1 cut(s)	605
XmnI	GAANNNNTTC	1 cut(s)	62
XspI	CTAG	6 cut(s)	360, 579, 624, 698, 960, 1347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.