Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008393

Physical Location & Seq

Genomic Coordinates Reverse (-)

24987460 .. 24992438

Gene Structure 4979 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1140700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2310 bp

ATGAGTACTGGGTTGGATCCGTCGATGACTTTGCTGGCAGCAAGCGGCGGCGACACCGTGAAGCTCTTCGATGTGTCGGACAAGTCCGCCGATCCCCTCGTTTTGAGCTACACTCCTTCTCCTGGTTCCCATGTCAATTCGCTCAAGTGGAATCACACTAATATGGTGGTGGCTAGTGCTGGAGATGACAAGAAGATATCGTTGTGGAATAAGAATGGGAAGAGCATGGGCACCATTCCGCTGGCCGGAACCGAAAGCGGAGACGGCATTGAGGAGTCTATATTGGCTCTCAGCTTCGGTAACAGGGTTTCTAGATACATCTGTTCGGGTGGAACCGGTCAGGTTGTAAGAATATGGGATTTGCAGAGAAAGCGGTGTATCAAATGGTTGAGGGGTCACTCTAGTACGATTACGGGTGCAATGTACAATTTCAAAGATGAGCACTTGGCTTCCATCAGTCTTAATGGGGACGTCATACTTCACAACCTTGCATCTGGTGCAAGGACTACTGAACTCAAGGACCCCAATCAACAGGTACTAAGAGTGCTTGACTATTCCCGGATTAGCCGACACCTTCTTGTGACAGCAGGTGATGGTGGGTCTGTACATTTGTGGGACACAACCGCTCGTAGCCCAAAGGTTTCTTGGCTGAAGCAGCATTCTGCACCAACTGCTGGTATCAGTTTCTCTCCATCTAATGACAAGGTGTTTGCTACAGTTGGGCTTGATAAGAAGTTGTATACTTACGACACAGGGTCGAGACAACCTTCACATTGCATTTCCTATAAAGCACCTTTCTCTTCATTGGCATTTAGAGGTGATGGTTGGGTATTGGCAGCTGGGACAAGCAGTGGTGATGTGGTGTTCTACGATGTCCGTGGTAAACCAGAGCCCTTCACTGGTATTCGTGCTTATAGTAGTTCAGAGGCTGTTACAAGTCTATGCTGGCAAAGGTCAAAACCTGTCGTTGTAAACGAAAGCAATTGTACTGTTGAGACTGCTCTTTTAGGAGATGCTGTTGAAGATTCAATCCTTATGCCAGACCAACTTCCTTCTGTGACTTCATCAAGTCTTTCTCTATCTATGGCAGTATCTAGTTCTCGAAATTCAGGCCGTTCAAGTTTATCTGCAGAGACAACTTCAGTTACAGCAGCTGGCAGTGGACTAACATCAAGCATACTTTGTGTATCTACTGGAGAGGATACACCGCAACGAGGTCGTTTATGGCCTGGTGGAACATTGCATAGATTGCATCCTCCTCGATCTACTTATAACTTCAAGGATGATATGGAGGTGTTCTCCCCTCTTGTGGACGTTCATCCAATCACACCTTCACTTGACAAACTTTGGGATGATGACAAATCAAAGAAGGATAATTTGTCCGGTAGGAGATTCCCTTTTGCAGAAGATGGGGCCAGTGATCATCCAATATTTGATTGGAAACCCAGTTCAACATCTACACAGGATGACATAAAATCGTCATTTGCACTATTAGAATCAACTCCTGCACCATCTTCCAAGAGCGAAGACCCATCCATCACCCCTCCAGAAGCTTGGGGTGGTGAGAGATTTTCTGATAAGTTCCGCCAGCCAATCACTTTTACATCTCGCATTGGGATGTCGGCTCAGACATCAGGACCAACTTTATCTGGACTGCCAGATATGTCTTTAACAGCAAAGTTTGCAAATTTGCGCACCAAAGATTTGTCTTCCATTCAGGAGACTTCATTAGGATTTCCAGAACACATTTCTTCGAGTGCTATGCCTATGTCCCCTGGTAACAAAGCCCTCTTGGGACAGGCTAACCTTGATTCACCAACATCTTTGACCTTGCCTCGGAGATTCTCAACTTATGCAGAGAGGATAAGCACTACTTCGAACTTCAGTGATCGAACGTCCCTAGCAGTGAGTTCGCCAAAAACAAAGAAAACAGGAGCCGAAGGCAGAGAAGAACTGTTGAATAGCATCTTGTCAAAGTCAGATACATCAACTGCCACAGAATCAGGGGTTCTTCCAGCAATGAATGGTGGAAGTTCACATCTCCAAAATGCACCCCAACAAGATGCACAGCAGGGAAGTTCCTTTACATTTCAGATTTTTCAACGTACTCTAGAAGAAACTCTCGATTCTTTTCAGAAATCCATACATGAAGATATGAGGAACCTTCATATAGAAATTTTAAGGCAATTTCATATGCAAGAGACAGAAATGTCAAATGTGATGACTGCAATGCTGGAAAACCAAGCTGAGCTGATGAAAGAAGTTAAGTCTCTCCGGAAAGAAAACCAGCAGCTCCGGCAATTGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000242 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0000922 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005814 GO:0005815 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0005929 GO:0007049 GO:0007275 GO:0007346 GO:0008104 GO:0008150 GO:0009553 GO:0009555 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0022402 GO:0022607 GO:0032465 GO:0032467 GO:0032501 GO:0032502 GO:0032506 GO:0032886 GO:0033036 GO:0033043 GO:0033365 GO:0034613 GO:0036064 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044380 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044463 GO:0044464 GO:0045787 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051179 GO:0051301 GO:0051302 GO:0051493 GO:0051641 GO:0051726 GO:0051781 GO:0060236 GO:0061640 GO:0065007 GO:0070507 GO:0070727 GO:0071539 GO:0071840 GO:0072698 GO:0090068 GO:0090224 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0120025 GO:0120038 GO:1902410 GO:1903047 GO:1905508 GO:2000694

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

770

Amino Acids

83.45

Weight (kDa)

6.34

Isoelectric Point (pI)

49.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	7 - 208	1.7e-23	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	10 - 237	1.1e-11	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	44 - 220	1.1e-15	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	92 - 241	6e-12	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	92 - 177	5.2e-06	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	103 - 250	1.1e-14	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	105 - 230	9.2e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	185 - 293	9.1e-06	WDR5 beta-propeller domain
WD40_Prp19	PF24814	189 - 296	7.3e-11	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	189 - 320	6.8e-06	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	191 - 325	2.2e-12	THOC3 beta-propeller domain
WD40_RFWD3	PF23419	213 - 315	5.6e-06	E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EML_2	PF23414	226 - 294	9.5e-07	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008393)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05970 AT5G05970
fragaria_vesca	FvH4_7g23510 FvH4_7g23510 FvH4_7g23510
malus_domestica	MD01G1140700.v1.1 MD07G1205000.v1.1
prunus_persica	Prupe.2G241200_v2.0.a1 Prupe.2G241200_v2.0.a1
pyrus_communis	pycom01g16130 pycom07g19150
rosa_chinensis	RchiOBHm_Chr1g0368661 RchiOBHm_Chr4g0410231
rosa_laevigata	RLG00000027199
rosa_multiflora	Rmu_co8334599.1_g000001 Rmu_sc0001791.1_g000011
rosa_roxburghii	Rroxscaffold_4G00288380
rosa_rugosa	Rorug01G0343400
rosa_samantha	Rh1AG350800 Rh1BG314500 Rh1CG329200 Rh1DG346100
rosa_wichuraiana	Rw0G021660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1272
AarI	CACCTGC	1 cut(s)	578
AasI	GACNNNNNNGTC	1 cut(s)	2209
AatII	GACGTC	1 cut(s)	474
Acc16I	TGCGCA	1 cut(s)	1694
Acc36I	ACCTGC	1 cut(s)	578
AccB1I	GGYRCC	1 cut(s)	230
AccB7I	CCANNNNNTGG	1 cut(s)	674
AccBSI	CCGCTC	1 cut(s)	626
AccI	GTMKAC	1 cut(s)	740
AccIII	TCCGGA	1 cut(s)	2274
AciI	CCGC	9 cut(s)	45, 48, 87, 239, 258, 373, 624, 1208, 1585
AclWI	GGATC	3 cut(s)	11, 24, 86
AcoI	YGGCCR	1 cut(s)	243
AcsI	RAATTY	3 cut(s)	1105, 1687, 2175
AcuI	CTGAAG	3 cut(s)	671, 1125, 1867
AcyI	GRCGYC	1 cut(s)	471
AfaI	GTAC	7 cut(s)	7, 406, 425, 537, 606, 988, 2107
AfiI	CCNNNNNNNGG	7 cut(s)	122, 245, 674, 899, 1214, 1309, 1836
AgeI	ACCGGT	1 cut(s)	335
AgsI	TTSAA	8 cut(s)	433, 1022, 1029, 1119, 1279, 1452, 1960, 2102
AhdI	GACNNNNNGTC	1 cut(s)	754
AjnI	CCWGG	3 cut(s)	121, 1228, 1774
AjuI	GAANNNNNNNTTGG	2 cut(s)	1315, 1347
AloI	GAACNNNNNNTCC	2 cut(s)	1432, 1464
AluBI	AGCT	9 cut(s)	64, 108, 294, 839, 1154, 1553, 2246, 2251, 2293
AluI	AGCT	9 cut(s)	64, 108, 294, 839, 1154, 1553, 2246, 2251, 2293
Alw21I	GWGCWC	1 cut(s)	444
Alw26I	GTCTC	7 cut(s)	255, 754, 989, 1127, 1715, 2195, 2274
AlwI	GGATC	3 cut(s)	11, 24, 86
AlwNI	CAGNNNCTG	2 cut(s)	929, 1154
Aor13HI	TCCGGA	1 cut(s)	2274
AoxI	GGCC	4 cut(s)	243, 1111, 1226, 1413
ApeKI	GCWGC	5 cut(s)	38, 655, 836, 1151, 2290
ApoI	RAATTY	3 cut(s)	1105, 1687, 2175
ArsI	GACNNNNNNTTYG	2 cut(s)	692, 724
AsiGI	ACCGGT	1 cut(s)	335
Asp700I	GAANNNNTTC	1 cut(s)	65
AspLEI	GCGC	1 cut(s)	1695
AspS9I	GGNCC	3 cut(s)	520, 1413, 1637
AsuC2I	CCSGG	1 cut(s)	559
AsuHPI	GGTGA	6 cut(s)	602, 830, 866, 1531, 1574, 1806
AsuII	TTCGAA	1 cut(s)	1877
AvaII	GGWCC	2 cut(s)	520, 1637
BaeGI	GKGCMC	1 cut(s)	233
BaeI	ACNNNNGTAYC	2 cut(s)	307, 340
BamHI	GGATCC	1 cut(s)	16
BanI	GGYRCC	1 cut(s)	230
BanII	GRGCYC	1 cut(s)	894
BarI	GAAGNNNNNNTAC	2 cut(s)	2068, 2100
BbsI	GAAGAC	2 cut(s)	1533, 1701
Bbv12I	GWGCWC	1 cut(s)	444
BbvI	GCAGC	5 cut(s)	50, 667, 848, 1163, 2302
BccI	CCATC	8 cut(s)	461, 587, 700, 815, 1403, 1519, 1540, 1544
BceAI	ACGGC	2 cut(s)	280, 1098
BciT130I	CCWGG	3 cut(s)	123, 1230, 1776
BciVI	GTATCC	1 cut(s)	1195
BclI	TGATCA	1 cut(s)	1420
BcnI	CCSGG	1 cut(s)	559
BcoDI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1715, 2195, 2274
BfaI	CTAG	6 cut(s)	174, 312, 402, 1095, 1901, 2111
BfmI	CTRYAG	2 cut(s)	714, 1128
BfuAI	ACCTGC	1 cut(s)	578
BfuI	GTATCC	1 cut(s)	1195
BisI	GCNGC	7 cut(s)	39, 46, 49, 656, 837, 1152, 2291
BlpI	GCTNAGC	1 cut(s)	2247
BlsI	GCNGC	7 cut(s)	40, 47, 50, 657, 838, 1153, 2292
BmcAI	AGTACT	1 cut(s)	7
Bme1390I	CCNGG	4 cut(s)	123, 559, 1230, 1776
Bme18I	GGWCC	2 cut(s)	520, 1637
BmeRI	GACNNNNNGTC	1 cut(s)	754
BmgT120I	GGNCC	3 cut(s)	520, 1413, 1637
BmiI	GGNNCC	9 cut(s)	18, 127, 232, 250, 334, 522, 1414, 1936, 2162
BmrFI	CCNGG	4 cut(s)	123, 559, 1230, 1776
BmrI	ACTGGG	2 cut(s)	18, 1440
BmsI	GCATC	5 cut(s)	500, 1003, 1261, 1974, 2053
BmuI	ACTGGG	2 cut(s)	18, 1440
BpiI	GAAGAC	2 cut(s)	1533, 1701
BplI	GAGNNNNNCTC	4 cut(s)	97, 129, 1283, 1315
BpmI	CTGGAG	3 cut(s)	201, 1215, 1530
Bpu1102I	GCTNAGC	1 cut(s)	2247
Bpu14I	TTCGAA	1 cut(s)	1877
BpuEI	CTTGAG	2 cut(s)	128, 500
BpuMI	CCSGG	1 cut(s)	559
BsaHI	GRCGYC	1 cut(s)	471
BsaJI	CCNNGG	3 cut(s)	877, 1774, 1835
BsaWI	WCCGGW	3 cut(s)	335, 1382, 2274
BsaXI	ACNNNNNCTCC	4 cut(s)	103, 133, 1002, 1032
Bsc4I	CCNNNNNNNGG	7 cut(s)	122, 245, 674, 899, 1214, 1309, 1836
Bse118I	RCCGGY	1 cut(s)	335
Bse1I	ACTGG	5 cut(s)	13, 904, 1198, 1416, 1446
Bse3DI	GCAATG	5 cut(s)	426, 772, 1238, 2025, 2235
BseAI	TCCGGA	1 cut(s)	2274
BseBI	CCWGG	3 cut(s)	123, 1230, 1776
BseDI	CCNNGG	3 cut(s)	877, 1774, 1835
BseGI	GGATG	8 cut(s)	1252, 1288, 1318, 1357, 1423, 1471, 1532, 1623
BseLI	CCNNNNNNNGG	7 cut(s)	122, 245, 674, 899, 1214, 1309, 1836
BseMI	GCAATG	5 cut(s)	426, 772, 1238, 2025, 2235
BseMII	CTCAG	3 cut(s)	304, 1640, 2238
BseNI	ACTGG	5 cut(s)	13, 904, 1198, 1416, 1446
BseRI	GAGGAG	2 cut(s)	287, 1248
BseSI	GKGCMC	1 cut(s)	233
BseXI	GCAGC	5 cut(s)	50, 667, 848, 1163, 2302
BseYI	CCCAGC	1 cut(s)	839
BsgI	GTGCAG	2 cut(s)	648, 1491
BshFI	GGCC	4 cut(s)	245, 1113, 1228, 1415
BshNI	GGYRCC	1 cut(s)	230
BshTI	ACCGGT	1 cut(s)	335
BsiHKAI	GWGCWC	1 cut(s)	444
BsiSI	CCGG	6 cut(s)	246, 336, 559, 1383, 2275, 2296
BslFI	GGGAC	6 cut(s)	482, 629, 856, 1756, 1809, 1882
BslI	CCNNNNNNNGG	7 cut(s)	122, 245, 674, 899, 1214, 1309, 1836
BsmAI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1715, 2195, 2274
BsmBI	CGTCTC	1 cut(s)	255
BsmFI	GGGAC	6 cut(s)	482, 629, 856, 1756, 1809, 1882
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	4 cut(s)	245, 1113, 1228, 1415
Bsp119I	TTCGAA	1 cut(s)	1877
Bsp1286I	GDGCHC	3 cut(s)	233, 444, 894
Bsp13I	TCCGGA	1 cut(s)	2274
Bsp1407I	TGTACA	2 cut(s)	423, 604
Bsp143I	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
Bsp1720I	GCTNAGC	1 cut(s)	2247
BspACI	CCGC	9 cut(s)	45, 48, 87, 239, 258, 373, 624, 1208, 1585
BspANI	GGCC	4 cut(s)	245, 1113, 1228, 1415
BspCNI	CTCAG	3 cut(s)	303, 1639, 2239
BspEI	TCCGGA	1 cut(s)	2274
BspLI	GGNNCC	9 cut(s)	18, 127, 232, 250, 334, 522, 1414, 1936, 2162
BspMAI	CTGCAG	1 cut(s)	1132
BspMI	ACCTGC	1 cut(s)	578
BspPI	GGATC	3 cut(s)	11, 24, 86
BspQI	GCTCTTC	2 cut(s)	71, 215
BspT104I	TTCGAA	1 cut(s)	1877
BspT107I	GGYRCC	1 cut(s)	230
BsrBI	CCGCTC	1 cut(s)	626
BsrDI	GCAATG	5 cut(s)	426, 772, 1238, 2025, 2235
BsrFI	RCCGGY	1 cut(s)	335
BsrGI	TGTACA	2 cut(s)	423, 604
BsrI	ACTGG	5 cut(s)	13, 904, 1198, 1416, 1446
BssAI	RCCGGY	1 cut(s)	335
BssECI	CCNNGG	3 cut(s)	877, 1774, 1835
BssMI	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
BssNAI	GTATAC	1 cut(s)	741
BssNI	GRCGYC	1 cut(s)	471
Bst1107I	GTATAC	1 cut(s)	741
Bst2UI	CCWGG	3 cut(s)	123, 1230, 1776
Bst4CI	ACNGT	4 cut(s)	58, 718, 991, 1956
Bst6I	CTCTTC	3 cut(s)	71, 215, 805
BstACI	GRCGYC	1 cut(s)	471
BstAPI	GCANNNNNTGC	4 cut(s)	497, 671, 1249, 1682
BstAUI	TGTACA	2 cut(s)	423, 604
BstBI	TTCGAA	1 cut(s)	1877
BstC8I	GCNNGC	6 cut(s)	36, 43, 243, 947, 1156, 1589
BstDEI	CTNAG	4 cut(s)	290, 539, 1626, 2247
BstDSI	CCRYGG	1 cut(s)	877
BstF5I	GGATG	8 cut(s)	1252, 1288, 1318, 1357, 1423, 1471, 1532, 1623
BstHHI	GCGC	1 cut(s)	1695
BstKTI	GATC	5 cut(s)	19, 94, 1265, 1423, 1891
BstMAI	GTCTC	7 cut(s)	255, 754, 989, 1127, 1715, 2195, 2274
BstMBI	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
BstMWI	GCNNNNNNNGC	8 cut(s)	264, 370, 497, 655, 671, 1249, 1682, 2296
BstNI	CCWGG	3 cut(s)	123, 1230, 1776
BstSCI	CCNGG	4 cut(s)	121, 557, 1228, 1774
BstSFI	CTRYAG	2 cut(s)	714, 1128
BstSLI	GKGCMC	1 cut(s)	233
BstV1I	GCAGC	5 cut(s)	50, 667, 848, 1163, 2302
BstV2I	GAAGAC	2 cut(s)	1533, 1701
BstX2I	RGATCY	1 cut(s)	16
BstXI	CCANNNNNNTGG	2 cut(s)	241, 1554
BstYI	RGATCY	1 cut(s)	16
BstZ17I	GTATAC	1 cut(s)	741
BsuI	GTATCC	1 cut(s)	1195
BsuRI	GGCC	4 cut(s)	245, 1113, 1228, 1415
BtgI	CCRYGG	1 cut(s)	877
BtsCI	GGATG	8 cut(s)	1252, 1288, 1318, 1357, 1423, 1471, 1532, 1623
BtsI	GCAGTG	3 cut(s)	856, 1165, 1911
BtsIMutI	CAGTG	6 cut(s)	856, 897, 1165, 1423, 1891, 1911
BveI	ACCTGC	1 cut(s)	578
Cac8I	GCNNGC	6 cut(s)	36, 43, 243, 947, 1156, 1589
CaiI	CAGNNNCTG	2 cut(s)	929, 1154
CfoI	GCGC	1 cut(s)	1695
Cfr10I	RCCGGY	1 cut(s)	335
Cfr13I	GGNCC	3 cut(s)	520, 1413, 1637
Csp6I	GTAC	7 cut(s)	6, 405, 424, 536, 605, 987, 2106
CspAI	ACCGGT	1 cut(s)	335
CviAII	CATG	3 cut(s)	131, 226, 2147
CviQI	GTAC	7 cut(s)	6, 405, 424, 536, 605, 987, 2106
DdeI	CTNAG	4 cut(s)	290, 539, 1626, 2247
DpnI	GATC	5 cut(s)	18, 93, 1264, 1422, 1890
DpnII	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
DrdI	GACNNNNNNGTC	1 cut(s)	2209
DriI	GACNNNNNGTC	1 cut(s)	754
DseDI	GACNNNNNNGTC	1 cut(s)	2209
EaeI	YGGCCR	1 cut(s)	243
Eam1104I	CTCTTC	3 cut(s)	71, 215, 805
Eam1105I	GACNNNNNGTC	1 cut(s)	754
EarI	CTCTTC	3 cut(s)	71, 215, 805
EciI	GGCGGA	2 cut(s)	76, 1574
Eco24I	GRGCYC	1 cut(s)	894
Eco32I	GATATC	1 cut(s)	198
Eco47I	GGWCC	2 cut(s)	520, 1637
Eco57I	CTGAAG	3 cut(s)	671, 1125, 1867
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRII	CCWGG	3 cut(s)	121, 1228, 1774
EcoRV	GATATC	1 cut(s)	198
EcoT38I	GRGCYC	1 cut(s)	894
Esp3I	CGTCTC	1 cut(s)	255
FaeI	CATG	3 cut(s)	134, 229, 2150
FalI	AAGNNNNNCTT	2 cut(s)	1858, 1890
FaqI	GGGAC	6 cut(s)	482, 629, 856, 1756, 1809, 1882
FatI	CATG	3 cut(s)	130, 225, 2146
FauNDI	CATATG	1 cut(s)	2193
FbaI	TGATCA	1 cut(s)	1420
FblI	GTMKAC	1 cut(s)	740
Fnu4HI	GCNGC	7 cut(s)	39, 46, 49, 656, 837, 1152, 2291
FokI	GGATG	8 cut(s)	1239, 1295, 1305, 1364, 1410, 1478, 1519, 1630
FriOI	GRGCYC	1 cut(s)	894
Fsp4HI	GCNGC	7 cut(s)	39, 46, 49, 656, 837, 1152, 2291
FspBI	CTAG	6 cut(s)	174, 312, 402, 1095, 1901, 2111
FspI	TGCGCA	1 cut(s)	1694
GlaI	GCGC	1 cut(s)	1694
GluI	GCNGC	7 cut(s)	39, 46, 49, 656, 837, 1152, 2291
GsaI	CCCAGC	1 cut(s)	843
GsuI	CTGGAG	3 cut(s)	201, 1215, 1530
HaeIII	GGCC	4 cut(s)	245, 1113, 1228, 1415
HapII	CCGG	6 cut(s)	246, 336, 559, 1383, 2275, 2296
HhaI	GCGC	1 cut(s)	1695
Hin1I	GRCGYC	1 cut(s)	471
Hin1II	CATG	3 cut(s)	134, 229, 2150
Hin6I	GCGC	1 cut(s)	1693
HinP1I	GCGC	1 cut(s)	1693
HindIII	AAGCTT	1 cut(s)	1551
HinfI	GANTC	9 cut(s)	151, 275, 1025, 1392, 1496, 1811, 1842, 2000, 2126
HpaII	CCGG	6 cut(s)	246, 336, 559, 1383, 2275, 2296
HphI	GGTGA	6 cut(s)	602, 830, 866, 1531, 1574, 1806
Hpy166II	GTNNAC	6 cut(s)	741, 884, 973, 1163, 1312, 2036
Hpy188I	TCNGA	8 cut(s)	79, 925, 1576, 1629, 1839, 1981, 2094, 2136
Hpy8I	GTNNAC	6 cut(s)	741, 884, 973, 1163, 1312, 2036
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	9 cut(s)	126, 584, 777, 904, 1062, 1341, 1363, 1934, 2174
HpyCH4III	ACNGT	4 cut(s)	58, 718, 991, 1956
HpyCH4IV	ACGT	4 cut(s)	471, 1314, 1895, 2104
HpyF10VI	GCNNNNNNNGC	8 cut(s)	264, 370, 497, 655, 671, 1249, 1682, 2296
HpyF3I	CTNAG	4 cut(s)	290, 539, 1626, 2247
HpySE526I	ACGT	4 cut(s)	471, 1314, 1895, 2104
Hsp92I	GRCGYC	1 cut(s)	471
Hsp92II	CATG	3 cut(s)	134, 229, 2150
HspAI	GCGC	1 cut(s)	1693
Kpn2I	TCCGGA	1 cut(s)	2274
Ksp22I	TGATCA	1 cut(s)	1420
Kzo9I	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
LguI	GCTCTTC	2 cut(s)	71, 215
LmnI	GCTCC	2 cut(s)	1934, 2298
Lsp1109I	GCAGC	5 cut(s)	50, 667, 848, 1163, 2302
LweI	GCATC	5 cut(s)	500, 1003, 1261, 1974, 2053
MaeI	CTAG	6 cut(s)	174, 312, 402, 1095, 1901, 2111
MaeII	ACGT	4 cut(s)	471, 1314, 1895, 2104
MaeIII	GTNAC	7 cut(s)	299, 395, 580, 931, 1057, 1144, 1778
MalI	GATC	5 cut(s)	18, 93, 1264, 1422, 1890
MbiI	CCGCTC	1 cut(s)	626
MboI	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
MfeI	CAATTG	2 cut(s)	982, 2300
MflI	RGATCY	1 cut(s)	16
MhlI	GDGCHC	3 cut(s)	233, 444, 894
MluCI	AATT	9 cut(s)	136, 427, 982, 1105, 1375, 1687, 2175, 2186, 2300
MlyI	GAGTC	1 cut(s)	284
MmeI	TCCRAC	1 cut(s)	57
MroI	TCCGGA	1 cut(s)	2274
MroXI	GAANNNNTTC	1 cut(s)	65
MseI	TTAA	4 cut(s)	462, 1670, 2180, 2265
MslI	CAYNNNNRTG	2 cut(s)	161, 1616
MspA1I	CMGCKG	3 cut(s)	241, 839, 1154
MspI	CCGG	6 cut(s)	246, 336, 559, 1383, 2275, 2296
MspR9I	CCNGG	4 cut(s)	123, 559, 1230, 1776
MunI	CAATTG	2 cut(s)	982, 2300
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	3 cut(s)	123, 1230, 1776
MwoI	GCNNNNNNNGC	8 cut(s)	264, 370, 497, 655, 671, 1249, 1682, 2296
NciI	CCSGG	1 cut(s)	559
NdeI	CATATG	1 cut(s)	2193
NdeII	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
NlaIII	CATG	3 cut(s)	134, 229, 2150
NlaIV	GGNNCC	9 cut(s)	18, 127, 232, 250, 334, 522, 1414, 1936, 2162
NmuCI	GTSAC	3 cut(s)	395, 580, 1057
NsbI	TGCGCA	1 cut(s)	1694
NspV	TTCGAA	1 cut(s)	1877
PaqCI	CACCTGC	1 cut(s)	578
PciSI	GCTCTTC	2 cut(s)	71, 215
PcsI	WCGNNNNNNNCGW	1 cut(s)	972
PctI	GAATGC	1 cut(s)	658
PdmI	GAANNNNTTC	1 cut(s)	65
PfeI	GAWTC	8 cut(s)	151, 1025, 1392, 1496, 1811, 1842, 2000, 2126
PflMI	CCANNNNNTGG	1 cut(s)	674
PfoI	TCCNGGA	1 cut(s)	557
PinAI	ACCGGT	1 cut(s)	335
PkrI	GCNGC	7 cut(s)	40, 47, 50, 657, 838, 1153, 2292
PleI	GAGTC	1 cut(s)	283
PpsI	GAGTC	1 cut(s)	283
PpuMI	RGGWCCY	1 cut(s)	520
PsiI	TTATAA	1 cut(s)	1272
Psp5II	RGGWCCY	1 cut(s)	520
Psp6I	CCWGG	3 cut(s)	121, 1228, 1774
PspFI	CCCAGC	1 cut(s)	839
PspGI	CCWGG	3 cut(s)	121, 1228, 1774
PspN4I	GGNNCC	9 cut(s)	18, 127, 232, 250, 334, 522, 1414, 1936, 2162
PspPI	GGNCC	3 cut(s)	520, 1413, 1637
PspPPI	RGGWCCY	1 cut(s)	520
PstI	CTGCAG	1 cut(s)	1132
PstNI	CAGNNNCTG	2 cut(s)	929, 1154
PsuI	RGATCY	1 cut(s)	16
PvuII	CAGCTG	2 cut(s)	839, 1154
RsaI	GTAC	7 cut(s)	7, 406, 425, 537, 606, 988, 2107
RsaNI	GTAC	7 cut(s)	6, 405, 424, 536, 605, 987, 2106
RseI	CAYNNNNRTG	2 cut(s)	161, 1616
SapI	GCTCTTC	2 cut(s)	71, 215
SaqAI	TTAA	4 cut(s)	462, 1670, 2180, 2265
SatI	GCNGC	7 cut(s)	39, 46, 49, 656, 837, 1152, 2291
Sau3AI	GATC	5 cut(s)	16, 91, 1262, 1420, 1888
Sau96I	GGNCC	3 cut(s)	520, 1413, 1637
ScaI	AGTACT	1 cut(s)	7
SchI	GAGTC	1 cut(s)	284
ScrFI	CCNGG	4 cut(s)	123, 559, 1230, 1776
SduI	GDGCHC	3 cut(s)	233, 444, 894
SfaNI	GCATC	5 cut(s)	500, 1003, 1261, 1974, 2053
SfcI	CTRYAG	2 cut(s)	714, 1128
SfuI	TTCGAA	1 cut(s)	1877
SinI	GGWCC	2 cut(s)	520, 1637
SmiMI	CAYNNNNRTG	2 cut(s)	161, 1616
SmlI	CTYRAG	2 cut(s)	143, 515
SmoI	CTYRAG	2 cut(s)	143, 515
Sse9I	AATT	9 cut(s)	136, 427, 982, 1105, 1375, 1687, 2175, 2186, 2300
SsiI	CCGC	9 cut(s)	45, 48, 87, 239, 258, 373, 624, 1208, 1585
SspI	AATATT	1 cut(s)	1431
SspMI	CTAG	6 cut(s)	174, 312, 402, 1095, 1901, 2111
StyD4I	CCNGG	4 cut(s)	121, 557, 1228, 1774
TaaI	ACNGT	4 cut(s)	58, 718, 991, 1956
TaiI	ACGT	4 cut(s)	474, 1317, 1898, 2107
TaqI	TCGA	9 cut(s)	23, 69, 758, 1102, 1261, 1754, 1877, 1891, 2124
TasI	AATT	9 cut(s)	136, 427, 982, 1105, 1375, 1687, 2175, 2186, 2300
TatI	WGTACW	4 cut(s)	5, 423, 604, 986
TauI	GCSGC	2 cut(s)	48, 51
TfiI	GAWTC	8 cut(s)	151, 1025, 1392, 1496, 1811, 1842, 2000, 2126
Tru1I	TTAA	4 cut(s)	462, 1670, 2180, 2265
Tru9I	TTAA	4 cut(s)	462, 1670, 2180, 2265
TscAI	CASTG	6 cut(s)	856, 904, 1165, 1423, 1891, 1911
TseFI	GTSAC	3 cut(s)	395, 580, 1057
TseI	GCWGC	5 cut(s)	38, 655, 836, 1151, 2290
Tsp45I	GTSAC	3 cut(s)	395, 580, 1057
TspDTI	ATGAA	9 cut(s)	792, 1053, 1307, 1716, 2036, 2156, 2163, 2180, 2270
TspGWI	ACGGA	2 cut(s)	9, 866
TspRI	CASTG	6 cut(s)	856, 904, 1165, 1423, 1891, 1911
Van91I	CCANNNNNTGG	1 cut(s)	674
VpaK11BI	GGWCC	2 cut(s)	520, 1637
XapI	RAATTY	3 cut(s)	1105, 1687, 2175
XbaI	TCTAGA	2 cut(s)	311, 2110
XcmI	CCANNNNNNNNNTGG	2 cut(s)	642, 1434
XmiI	GTMKAC	1 cut(s)	740
XmnI	GAANNNNTTC	1 cut(s)	65
XspI	CTAG	6 cut(s)	174, 312, 402, 1095, 1901, 2111
ZraI	GACGTC	1 cut(s)	472
ZrmI	AGTACT	1 cut(s)	7

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.