FvH4_7g24530

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006817
Physical Location & Seq
Reverse (-)
18901535 .. 18906350
4816 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24530.t4

Sequence Viewer

Length: 2037 bp
ATGGAGGTCGAGGCAGTGCTCGAGTTTCTGAAGAAACACGGCTTTTCAGAGGCGGAGTCGGCTCTGAGAGAGGATATGATTGAAAAGGGCGATCTGGGTTCGTTTGACTATGAGAAATTCTTGTTTCCGATGGCTCCTCCTCTGCCGCCGGTGAGGATTCGGTCGAGTTTTCAGAGATCGAAGGTTTTAGGTGTGGTTGAAGTGTCGAGCTCGCGATCGAGTTCCGGCGATGATGAGTTTGTGAGTTTGGGGTCTTCTACTACTAATAGGTGCTCTTCAGATTTTACAAATCCATATGGAGTTCGATCTACATCTCCAGGCAGTTCCAAGGCTTCATCTGATGGATTCTCACAATTTGGCACTGCACGCAATTACCATGATTTCGATAAGCAAAATGACTTGTTGGGGCATGATGAGAAAGACGATGCAGACTACATGACTCCTTGCTTTGACGGGGTAGACTACTTTGGCTGTCCCACTGAGGATAAGTTCATCATGACTTCAGACACTGACAAGAAAAACGAGGACACGCTGGATCTGCATTATAAATCTGAAGCATTTCCATCCAAAATAAGCGTTGATTACTTGGACAAGAAATGTCTTACTAATATATCTTCCATAGATGCCAAAGGTGTCTATGTGACAAACTACTATCAATATGAAATGCAACATCTGCTTGAAGGAGGCTCTGAGGAGGAGCCTGCTGGTGAAAAGTTGAACGAAACTAATTTGGATGATTGTCATTTGGATGTTTTCGGCGGTTTTCCTTCCACCAATAGAAGAAGGAAGAGTTCTAGCTACTCTCCTAAAAGGAGTTCAACAAATGGCTGGTTGGAAAAAGGTTCTTCTGATTTACATGGTAAGGTTGCAGAGAAAGATCTTAGGCCGAATGGAATTGACAGCTCTGAAGTTGGAAACAGTGATGTAAATGAAGAGTCCCATGATCCTGAGGCTGCTACTGATGAAGACAGTAATGAAGTTTTTATGCAGAACACTGATGAGAATGAGTATGAAGTTTTCGATTTAAGAGTCATACACAGGAAAAACAGGACCGGATTTGAAGCAAACAAGGATCTGCCTATTGTGCTAAAGACCATAGTTGCAGGCAGATATTATATAACTGAATATTTGGGTTCGGCTGCTTTCAGTAGGGTTGTACAGGCTCATGATCTTCACACAGGAGTTGATGTTTGTCTAAAGATCATAAAAAATGATAAAGATTTTTTTGATCAAAGTTTAGATGAGATCAAACTTCTTAAGTTTGTAAACAAGTATGACCCTGCAGATGAGCGCCACATTCTGCGACTTTATGACTACTTCTATCACCAGGAGCATCTCTTCATTGTTTGTGAACTACTCAAGGCGAATTTGTACGAATTTCAGAAGTTCAGTCGAGAATCTGGTGGAGAAGCATACTTTACATTAAAGAGGTTGCAGGCCATAACTCGTCAATGTTTAGAGGCATTGGAATACTTGCATCACCTGGGTGTTATTCACTGCGATCTCAAGCCTGAGAACATTCTCATCAAAAGTTACAGAAGATGTGAGATAAAAGTCATTGATCTCGGAAGCAGTTGCTTTCGAACAGACAACTTATGCCTATATGTACAATCTCGTTCCTACAGAGCTCCTGAAGTCATGCTTGGGCTCTCATATGACGAGAAGATTGACATGTGGTCTCTTGGATGCATCTTGGCTGAGCTATGTTCTGGAGAAGTGCTCTTTCCAAATGATGGGATTGTGACCATCCTTGCACGTATCATTGGAATGCTTGGTCCTATTGATCTGGATATGCTAGTGAAAGGACAGGAAACAGACAAGTACTTCACAAACGAATTAGATCTCTACCACATAAATGAGGAGACGAACCAATTGGAATACATAATCCCTGAGGACTCCTCCCTGGAGCAACACCTGGAAGTAACTGATATTGGCTTCATTGACTTTGTCCAAAGCTTGCTCGAGATCAATCCTGAGAGACGGCCAAGTGCAACGGAGGCACTAGAGCACCCCTGGCTTTCCTATTCATATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

679

Amino Acids

77.2

Weight (kDa)

4.75

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 373 - 573 3.2e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 374 - 571 6.3e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 1733
AccI GTMKAC 1 cut(s) 459
AccII CGCG 1 cut(s) 214
AciI CCGC 3 cut(s) 53, 146, 759
AclWI GGATC 3 cut(s) 543, 938, 1080
AcoI YGGCCR 1 cut(s) 1982
AcsI RAATTY 3 cut(s) 116, 1366, 1376
AcuI CTGAAG 6 cut(s) 50, 261, 486, 573, 927, 1653
AfaI GTAC 4 cut(s) 1158, 1373, 1608, 1823
AfiI CCNNNNNNNGG 1 cut(s) 1733
AflII CTTAAG 1 cut(s) 1256
AflIII ACRYGT 1 cut(s) 1671
AgsI TTSAA 6 cut(s) 83, 200, 680, 718, 819, 1061
AhdI GACNNNNNGTC 1 cut(s) 1675
AjnI CCWGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
AjuI GAANNNNNNNTTGG 2 cut(s) 1626, 1658
AleI CACNNNNGTG 1 cut(s) 1485
AluBI AGCT 6 cut(s) 210, 798, 903, 1628, 1702, 1956
AluI AGCT 6 cut(s) 210, 798, 903, 1628, 1702, 1956
Alw21I GWGCWC 6 cut(s) 21, 212, 275, 1630, 1722, 2010
Alw26I GTCTC 3 cut(s) 1683, 1856, 1972
AlwI GGATC 3 cut(s) 543, 938, 1080
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 2 cut(s) 20, 1961
AoxI GGCC 3 cut(s) 884, 1437, 1982
ApeKI GCWGC 2 cut(s) 953, 1139
ApoI RAATTY 3 cut(s) 116, 1366, 1376
Asp700I GAANNNNTTC 1 cut(s) 558
AspLEI GCGC 1 cut(s) 1293
AspS9I GGNCC 2 cut(s) 1050, 1775
AsuHPI GGTGA 4 cut(s) 163, 719, 1316, 1472
AsuII TTCGAA 1 cut(s) 1582
AvaI CYCGRG 2 cut(s) 20, 1961
AvaII GGWCC 2 cut(s) 1050, 1775
AxyI CCTNAGG 2 cut(s) 948, 1890
BanII GRGCYC 3 cut(s) 212, 1630, 1650
BbsI GAAGAC 2 cut(s) 246, 972
Bbv12I GWGCWC 6 cut(s) 21, 212, 275, 1630, 1722, 2010
BbvI GCAGC 2 cut(s) 940, 1126
BccI CCATC 5 cut(s) 124, 335, 571, 1727, 1754
BceAI ACGGC 2 cut(s) 55, 1997
BciT130I CCWGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
BclI TGATCA 1 cut(s) 1228
BcoDI GTCTC 3 cut(s) 1683, 1856, 1972
BfaI CTAG 3 cut(s) 795, 1796, 2003
BfmI CTRYAG 2 cut(s) 1281, 1621
BfoI RGCGCY 1 cut(s) 1294
BfrI CTTAAG 1 cut(s) 1256
BglII AGATCT 2 cut(s) 877, 1840
BisI GCNGC 3 cut(s) 146, 954, 1140
BlpI GCTNAGC 1 cut(s) 1698
BlsI GCNGC 3 cut(s) 147, 955, 1141
BmcAI AGTACT 1 cut(s) 1823
Bme1390I CCNGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
Bme18I GGWCC 2 cut(s) 1050, 1775
BmeRI GACNNNNNGTC 1 cut(s) 1675
BmeT110I CYCGRG 2 cut(s) 20, 1961
BmgT120I GGNCC 2 cut(s) 1050, 1775
BmiI GGNNCC 2 cut(s) 135, 699
BmrFI CCNGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
BmsI GCATC 6 cut(s) 415, 613, 1342, 1486, 1676, 1698
BpiI GAAGAC 2 cut(s) 246, 972
BplI GAGNNNNNCTC 4 cut(s) 1704, 1736, 1883, 1915
BpmI CTGGAG 3 cut(s) 300, 1731, 1925
Bpu1102I GCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 1582
BpuEI CTTGAG 2 cut(s) 1343, 1490
BsaAI YACGTR 1 cut(s) 1757
BsaBI GATNNNNATC 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 1683
BsaJI CCNNGG 4 cut(s) 327, 1483, 1902, 2012
BsaWI WCCGGW 1 cut(s) 1052
BsaXI ACNNNNNCTCC 2 cut(s) 1173, 1203
Bsc4I CCNNNNNNNGG 1 cut(s) 1733
Bse118I RCCGGY 1 cut(s) 148
Bse21I CCTNAGG 2 cut(s) 948, 1890
Bse8I GATNNNNATC 1 cut(s) 310
BseBI CCWGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
BseDI CCNNGG 4 cut(s) 327, 1483, 1902, 2012
BseGI GGATG 5 cut(s) 563, 739, 754, 1691, 1746
BseJI GATNNNNATC 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 1733
BseMII CTCAG 8 cut(s) 56, 471, 681, 939, 1503, 1689, 1881, 1965
BseRI GAGGAG 6 cut(s) 126, 129, 707, 710, 1874, 1888
BseXI GCAGC 2 cut(s) 940, 1126
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 2 cut(s) 164, 218
BshFI GGCC 3 cut(s) 886, 1439, 1984
BsiEI CGRYCG 2 cut(s) 164, 218
BsiHKAI GWGCWC 6 cut(s) 21, 212, 275, 1630, 1722, 2010
BsiHKCI CYCGRG 2 cut(s) 20, 1961
BsiSI CCGG 3 cut(s) 149, 225, 1053
BslFI GGGAC 2 cut(s) 459, 922
BslI CCNNNNNNNGG 1 cut(s) 1733
BsmAI GTCTC 3 cut(s) 1683, 1856, 1972
BsmBI CGTCTC 2 cut(s) 1856, 1972
BsmFI GGGAC 2 cut(s) 459, 922
BsmI GAATGC 1 cut(s) 1773
BsnI GGCC 3 cut(s) 886, 1439, 1984
Bso31I GGTCTC 1 cut(s) 1683
BsoBI CYCGRG 2 cut(s) 20, 1961
Bsp119I TTCGAA 1 cut(s) 1582
Bsp1286I GDGCHC 7 cut(s) 21, 212, 275, 1630, 1650, 1722, 2010
Bsp1407I TGTACA 2 cut(s) 1156, 1606
Bsp1720I GCTNAGC 1 cut(s) 1698
Bsp68I TCGCGA 1 cut(s) 214
BspACI CCGC 3 cut(s) 53, 146, 759
BspANI GGCC 3 cut(s) 886, 1439, 1984
BspCNI CTCAG 8 cut(s) 57, 472, 682, 940, 1504, 1690, 1882, 1966
BspFNI CGCG 1 cut(s) 214
BspHI TCATGA 2 cut(s) 495, 1165
BspLI GGNNCC 2 cut(s) 135, 699
BspMAI CTGCAG 1 cut(s) 1285
BspPI GGATC 3 cut(s) 543, 938, 1080
BspQI GCTCTTC 1 cut(s) 280
BspT104I TTCGAA 1 cut(s) 1582
BspTI CTTAAG 1 cut(s) 1256
BspTNI GGTCTC 1 cut(s) 1683
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 2 cut(s) 1156, 1606
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 327, 1483, 1902, 2012
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
Bst4CI ACNGT 2 cut(s) 920, 971
Bst6I CTCTTC 4 cut(s) 280, 782, 927, 1343
BstAFI CTTAAG 1 cut(s) 1256
BstAPI GCANNNNNTGC 1 cut(s) 673
BstAUI TGTACA 2 cut(s) 1156, 1606
BstBAI YACGTR 1 cut(s) 1757
BstBI TTCGAA 1 cut(s) 1582
BstC8I GCNNGC 6 cut(s) 212, 367, 702, 1105, 1437, 1958
BstDEI CTNAG 9 cut(s) 65, 480, 690, 881, 948, 1512, 1698, 1890, 1974
BstF5I GGATG 5 cut(s) 563, 739, 754, 1691, 1746
BstFNI CGCG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 1294
BstHHI GCGC 1 cut(s) 1293
BstMAI GTCTC 3 cut(s) 1683, 1856, 1972
BstMCI CGRYCG 2 cut(s) 164, 218
BstMWI GCNNNNNNNGC 7 cut(s) 59, 366, 538, 673, 1084, 1997, 2014
BstNI CCWGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
BstNSI RCATGY 1 cut(s) 1675
BstSCI CCNGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
BstSFI CTRYAG 2 cut(s) 1281, 1621
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 2 cut(s) 940, 1126
BstV2I GAAGAC 2 cut(s) 246, 972
BstX2I RGATCY 4 cut(s) 535, 877, 1072, 1840
BstYI RGATCY 4 cut(s) 535, 877, 1072, 1840
Bsu36I CCTNAGG 2 cut(s) 948, 1890
BsuRI GGCC 3 cut(s) 886, 1439, 1984
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 5 cut(s) 563, 739, 754, 1691, 1746
BtsI GCAGTG 3 cut(s) 21, 360, 1495
BtsIMutI CAGTG 7 cut(s) 21, 360, 477, 507, 925, 993, 1495
BtuMI TCGCGA 1 cut(s) 214
Cac8I GCNNGC 6 cut(s) 212, 367, 702, 1105, 1437, 1958
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 2 cut(s) 495, 1165
CfoI GCGC 1 cut(s) 1293
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 1050, 1775
Csp6I GTAC 4 cut(s) 1157, 1372, 1607, 1822
CviAII CATG 9 cut(s) 377, 410, 436, 496, 857, 941, 1166, 1639, 1672
CviQI GTAC 4 cut(s) 1157, 1372, 1607, 1822
DdeI CTNAG 9 cut(s) 65, 480, 690, 881, 948, 1512, 1698, 1890, 1974
DriI GACNNNNNGTC 1 cut(s) 1675
EaeI YGGCCR 1 cut(s) 1982
Eam1104I CTCTTC 4 cut(s) 280, 782, 927, 1343
Eam1105I GACNNNNNGTC 1 cut(s) 1675
EarI CTCTTC 4 cut(s) 280, 782, 927, 1343
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 2 cut(s) 210, 1628
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 3 cut(s) 212, 1630, 1650
Eco31I GGTCTC 1 cut(s) 1683
Eco47I GGWCC 2 cut(s) 1050, 1775
Eco53kI GAGCTC 2 cut(s) 210, 1628
Eco57I CTGAAG 6 cut(s) 50, 261, 486, 573, 927, 1653
Eco81I CCTNAGG 2 cut(s) 948, 1890
Eco88I CYCGRG 2 cut(s) 20, 1961
EcoICRI GAGCTC 2 cut(s) 210, 1628
EcoRII CCWGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 1691
EcoT38I GRGCYC 3 cut(s) 212, 1630, 1650
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 2 cut(s) 1856, 1972
FaeI CATG 9 cut(s) 380, 413, 439, 499, 860, 944, 1169, 1642, 1675
FalI AAGNNNNNCTT 2 cut(s) 1626, 1658
FaqI GGGAC 2 cut(s) 459, 922
FatI CATG 9 cut(s) 376, 409, 435, 495, 856, 940, 1165, 1638, 1671
FauNDI CATATG 3 cut(s) 295, 1654, 2029
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 3 cut(s) 146, 954, 1140
FokI GGATG 5 cut(s) 550, 746, 761, 1698, 1733
FriOI GRGCYC 3 cut(s) 212, 1630, 1650
Fsp4HI GCNGC 3 cut(s) 146, 954, 1140
FspBI CTAG 3 cut(s) 795, 1796, 2003
GlaI GCGC 1 cut(s) 1292
GluI GCNGC 3 cut(s) 146, 954, 1140
GsuI CTGGAG 3 cut(s) 300, 1731, 1925
HaeII RGCGCY 1 cut(s) 1294
HaeIII GGCC 3 cut(s) 886, 1439, 1984
HapII CCGG 3 cut(s) 149, 225, 1053
HhaI GCGC 1 cut(s) 1293
Hin1II CATG 9 cut(s) 380, 413, 439, 499, 860, 944, 1169, 1642, 1675
Hin6I GCGC 1 cut(s) 1291
HinP1I GCGC 1 cut(s) 1291
HindIII AAGCTT 1 cut(s) 1954
HinfI GANTC 8 cut(s) 56, 157, 345, 439, 935, 1029, 1397, 1895
HpaII CCGG 3 cut(s) 149, 225, 1053
HphI GGTGA 4 cut(s) 163, 719, 1316, 1472
Hpy166II GTNNAC 3 cut(s) 460, 1267, 1352
Hpy8I GTNNAC 3 cut(s) 460, 1267, 1352
HpyAV CCTTC 4 cut(s) 175, 674, 777, 777
HpyCH4III ACNGT 2 cut(s) 920, 971
HpyCH4IV ACGT 1 cut(s) 1756
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 366, 538, 673, 1084, 1997, 2014
HpyF3I CTNAG 9 cut(s) 65, 480, 690, 881, 948, 1512, 1698, 1890, 1974
HpySE526I ACGT 1 cut(s) 1756
Hsp92II CATG 9 cut(s) 380, 413, 439, 499, 860, 944, 1169, 1642, 1675
HspAI GCGC 1 cut(s) 1291
Ksp22I TGATCA 1 cut(s) 1228
LguI GCTCTTC 1 cut(s) 280
LmnI GCTCC 5 cut(s) 139, 697, 1330, 1633, 1906
Lsp1109I GCAGC 2 cut(s) 940, 1126
LweI GCATC 6 cut(s) 415, 613, 1342, 1486, 1676, 1698
MaeI CTAG 3 cut(s) 795, 1796, 2003
MaeII ACGT 1 cut(s) 1756
MaeIII GTNAC 4 cut(s) 640, 1532, 1741, 1921
MfeI CAATTG 1 cut(s) 1871
MflI RGATCY 4 cut(s) 535, 877, 1072, 1840
MhlI GDGCHC 7 cut(s) 21, 212, 275, 1630, 1650, 1722, 2010
MluCI AATT 9 cut(s) 116, 353, 370, 727, 894, 1366, 1376, 1835, 1871
MlyI GAGTC 5 cut(s) 65, 433, 944, 1038, 1889
MmeI TCCRAC 2 cut(s) 813, 892
Mph1103I ATGCAT 1 cut(s) 1691
MroXI GAANNNNTTC 1 cut(s) 558
MseI TTAA 3 cut(s) 1025, 1257, 1424
MslI CAYNNNNRTG 4 cut(s) 747, 1485, 1766, 1854
MspCI CTTAAG 1 cut(s) 1256
MspI CCGG 3 cut(s) 149, 225, 1053
MspR9I CCNGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
MunI CAATTG 1 cut(s) 1871
Mva1269I GAATGC 1 cut(s) 1773
MvaI CCWGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 7 cut(s) 59, 366, 538, 673, 1084, 1997, 2014
NdeI CATATG 3 cut(s) 295, 1654, 2029
NlaIII CATG 9 cut(s) 380, 413, 439, 499, 860, 944, 1169, 1642, 1675
NlaIV GGNNCC 2 cut(s) 135, 699
NmuCI GTSAC 2 cut(s) 640, 1741
NruI TCGCGA 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 1691
NspI RCATGY 1 cut(s) 1675
NspV TTCGAA 1 cut(s) 1582
OliI CACNNNNGTG 1 cut(s) 1485
PaeR7I CTCGAG 2 cut(s) 20, 1961
PagI TCATGA 2 cut(s) 495, 1165
PciI ACATGT 1 cut(s) 1671
PciSI GCTCTTC 1 cut(s) 280
PctI GAATGC 1 cut(s) 1773
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 3 cut(s) 157, 345, 1397
PflFI GACNNNGTC 1 cut(s) 1946
PflMI CCANNNNNTGG 1 cut(s) 1733
PkrI GCNGC 3 cut(s) 147, 955, 1141
Ple19I CGATCG 1 cut(s) 218
PleI GAGTC 5 cut(s) 64, 433, 943, 1037, 1889
PpsI GAGTC 5 cut(s) 64, 433, 943, 1037, 1889
Ppu21I YACGTR 1 cut(s) 1757
PscI ACATGT 1 cut(s) 1671
PsiI TTATAA 1 cut(s) 546
Psp124BI GAGCTC 2 cut(s) 212, 1630
Psp6I CCWGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
PspGI CCWGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
PspN4I GGNNCC 2 cut(s) 135, 699
PspPI GGNCC 2 cut(s) 1050, 1775
PspXI VCTCGAGB 1 cut(s) 20
PstI CTGCAG 1 cut(s) 1285
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 4 cut(s) 535, 877, 1072, 1840
PsyI GACNNNGTC 1 cut(s) 1946
PvuI CGATCG 1 cut(s) 218
RruI TCGCGA 1 cut(s) 214
RsaI GTAC 4 cut(s) 1158, 1373, 1608, 1823
RsaNI GTAC 4 cut(s) 1157, 1372, 1607, 1822
RseI CAYNNNNRTG 4 cut(s) 747, 1485, 1766, 1854
SacI GAGCTC 2 cut(s) 212, 1630
SapI GCTCTTC 1 cut(s) 280
SaqAI TTAA 3 cut(s) 1025, 1257, 1424
SatI GCNGC 3 cut(s) 146, 954, 1140
Sau96I GGNCC 2 cut(s) 1050, 1775
ScaI AGTACT 1 cut(s) 1823
SchI GAGTC 5 cut(s) 65, 433, 944, 1038, 1889
ScrFI CCNGG 6 cut(s) 318, 1328, 1484, 1904, 1916, 2014
SduI GDGCHC 7 cut(s) 21, 212, 275, 1630, 1650, 1722, 2010
SfaNI GCATC 6 cut(s) 415, 613, 1342, 1486, 1676, 1698
SfcI CTRYAG 2 cut(s) 1281, 1621
Sfr274I CTCGAG 2 cut(s) 20, 1961
SfuI TTCGAA 1 cut(s) 1582
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 2 cut(s) 1050, 1775
SlaI CTCGAG 2 cut(s) 20, 1961
SmiMI CAYNNNNRTG 4 cut(s) 747, 1485, 1766, 1854
SmlI CTYRAG 5 cut(s) 20, 1256, 1358, 1505, 1961
SmoI CTYRAG 5 cut(s) 20, 1256, 1358, 1505, 1961
Sse9I AATT 9 cut(s) 116, 353, 370, 727, 894, 1366, 1376, 1835, 1871
SsiI CCGC 3 cut(s) 53, 146, 759
SspI AATATT 1 cut(s) 1127
SspMI CTAG 3 cut(s) 795, 1796, 2003
SstI GAGCTC 2 cut(s) 212, 1630
StyD4I CCNGG 6 cut(s) 316, 1326, 1482, 1902, 1914, 2012
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 920, 971
TaiI ACGT 1 cut(s) 1759
TaqII GACCGA 1 cut(s) 150
TasI AATT 9 cut(s) 116, 353, 370, 727, 894, 1366, 1376, 1835, 1871
TatI WGTACW 3 cut(s) 1156, 1606, 1821
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 3 cut(s) 157, 345, 1397
Tru1I TTAA 3 cut(s) 1025, 1257, 1424
Tru9I TTAA 3 cut(s) 1025, 1257, 1424
TscAI CASTG 7 cut(s) 21, 367, 484, 514, 925, 1000, 1502
TseFI GTSAC 2 cut(s) 640, 1741
TseI GCWGC 2 cut(s) 953, 1139
Tsp45I GTSAC 2 cut(s) 640, 1741
TspGWI ACGGA 1 cut(s) 2009
TspRI CASTG 7 cut(s) 21, 367, 484, 514, 925, 1000, 1502
Tth111I GACNNNGTC 1 cut(s) 1946
Van91I CCANNNNNTGG 1 cut(s) 1733
Vha464I CTTAAG 1 cut(s) 1256
VpaK11BI GGWCC 2 cut(s) 1050, 1775
XapI RAATTY 3 cut(s) 116, 1366, 1376
XceI RCATGY 1 cut(s) 1675
XhoI CTCGAG 2 cut(s) 20, 1961
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 3 cut(s) 795, 1796, 2003
ZrmI AGTACT 1 cut(s) 1823
Zsp2I ATGCAT 1 cut(s) 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.