MD01G1153300.v1.1

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006817
Physical Location & Seq
Reverse (-)
26154170 .. 26158541
4372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1153300.v1.1.491

Sequence Viewer

Length: 2091 bp
ATGGGGGACTCGGCCGACGTCTCGGCGGTGCTCGAATTCCTGAGGGCAAACGGGTTTTCGGAGGCCGAATCTGCTCTCAAAGAGGACATGATTGAGAAAGGCGAACTGGGTTATTTCGATTACGAGAAGTTCTTGTTTCCGATGGTTCCTCCGCTGCCGCCGGTCAGAATTCCGGCGAGTTTCCGACGACCGGAGGTTCAGGGCTGCGTAGAATGCTCGGGATCTAGCTCGGCGGAGGACGATGAGTTTGTGAGCTTGGGGTCTTCTACTTCTAACAAGTGCTCTTCAGAATTTACAAATCCGTATGGAGTTCGATCAACATCTCCAAGCAGTTCCCAGGCTTCATCTGATAGGTTCTCTCAGTTTGGCACGGCACGCGATTACCACGATTTCGATATGCAAAATGACCTGTTCTGGCAAGATGAAAAAGATGATGGGGGAGACTTCATGACTCCTTGCCTTGAAGGGCCAGACTTCTTCGCTTGTCCCACTGAGGATAAGTTCATCATGACTTCCGACACAGACAAGGGGAACGAGAAAGTGCTGGGTCTGAATTATAAATCGGAAGCATTTCAATCGGAAATAAGCCTTGATTGCTTGGACAAGAGTTGTCTTACCGATGTTTCTCCCGTGGATGATACAAGCGAGATTTATGCGACGAATTATTATCATTTTGATAAGAAAAATCAGTTGGAAGAAGGCTTTGAGGAACATTCTGCAGAATGCTGCTATCCAAGTTTGAAAGAAACTGATTTCAACAATTGCCAATTAGATGTTTCGGGGGGTATCCTTCGTATGGATATTGATTCTGCTCCATATCACAAGTTAAGCAACAGTTCTAATTACTCGGCCAAAAGGAGTTTGAAAAATGGCCAGGGTGACAAGTTTATGGGGTCCTCTGGTTTAGACGATAAGGTTGCAGTGAAGGATTTCATGCCAAGGGGAGTTAAAGGATATGAAGGAGTCGGTGAAGTAAAAAAAGGATCCCACGAGCCCGAGGTTGGTGCTGATGGAGATGGTGTTGCTCCCGATGAGGTTTTGATGTACAATAATGACGAGGATTATGAAGTTTTTGAGTTGAGAATCGTACACAGGAAGAACAGGACCGGATTTGAAGAAAGCAAGGATTTCCCTATTGTGCTAAAGACTGTAATTGCAAGCAGATACTATGTAACGGAATATCTTGGTTCAGCAGCTTTCAGTAAGGTTGTACAGGCTCATGATCTTCACGCAGGAGTCGATGTTTGCCTTAAGATCATAAAGAATGATAAAGAATTTTTCGATCAGAGTCTGGATGAGATTAAACTTCTAAAGTTTGCCAACAAAAATGACCCTGCAGACGAACACCACATTTTGCGACTTTATGACTACTTCTATCATCAGGAGCATCTCTTCATTGTATGCGAACTCCTTCGGGCAAACTTGTATGAATTTCAGAAGTTCAGTCAAGAATCTGGTGGAGAAGCTTACTTCACAATAAGAAGGTTGCAGATCATAACCCGTCAATGTTTAGAGGCATTGGACTATTTGCATCGCTTGGGAATTATTCACTGTGATCTAAAGCCTGAAAACATTCTTATAAAAAGTTACAGAAGATGTGAAATAAAGATAATTGATCTTGGAAGCAGTTGCTTTCGCACGGACAACTTGTGTCTATATGTGCAATCTCGTTCATATAGAGCTCCTGAAGTCATACTTGGCCTCCCTTATGACGAGAAGATTGACATGTGGTCTCTTGGCTGTATATTGGCGGAGTTATGCTCCGGAGAAGTGCTCTTTCCAAATGACGGGGTTGTTATGATCCTTGCGCGCATGATTGGAATGCTTGGTCCTATCGATCTGGATATGTTAGTGAAAGGTCAGGAGACCGATAAGTACTTCACAAACGAGTTAGATCTTTACCACATAAATGAGGAAACCAGCCAACTGGAATACATAATCCCTGAGGAGTCCTCCTTGGAGAATCACCTGCAAGTAACCGACATTGGGTTTATCGACTTTGTCAAAAGCCTGCTTGAAGTCAATCCTGAGAGACGCCCAACCGCAAGGGAAGCACTAGAGCACCCATGGCTTTCCTACACATACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

697

Amino Acids

78.97

Weight (kDa)

4.63

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 390 - 691 2.4e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 392 - 590 2e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 558, 1580
AarI CACCTGC 1 cut(s) 1977
AatII GACGTC 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 1977
AccII CGCG 2 cut(s) 378, 1810
AccIII TCCGGA 1 cut(s) 1763
AciI CCGC 6 cut(s) 26, 152, 158, 233, 1751, 2043
AclWI GGATC 4 cut(s) 229, 978, 991, 1795
AcoI YGGCCR 3 cut(s) 12, 849, 871
AcsI RAATTY 5 cut(s) 35, 168, 290, 1274, 1430
AcuI CTGAAG 2 cut(s) 270, 1707
AcyI GRCGYC 2 cut(s) 18, 2035
AfaI GTAC 4 cut(s) 1046, 1089, 1212, 1877
AfiI CCNNNNNNNGG 5 cut(s) 190, 796, 1001, 1787, 1986
AflII CTTAAG 1 cut(s) 1250
AflIII ACRYGT 1 cut(s) 1725
AgsI TTSAA 7 cut(s) 464, 575, 742, 757, 865, 1115, 2018
AhdI GACNNNNNGTC 1 cut(s) 1729
AjnI CCWGG 2 cut(s) 336, 873
AjuI GAANNNNNNNTTGG 4 cut(s) 674, 706, 1680, 1712
AluBI AGCT 5 cut(s) 228, 255, 1196, 1466, 1682
AluI AGCT 5 cut(s) 228, 255, 1196, 1466, 1682
Alw21I GWGCWC 5 cut(s) 33, 284, 1684, 1776, 2064
Alw26I GTCTC 5 cut(s) 25, 435, 1737, 1859, 2026
AlwI GGATC 4 cut(s) 229, 978, 991, 1795
AlwNI CAGNNNCTG 1 cut(s) 1291
Ama87I CYCGRG 2 cut(s) 217, 995
Aor13HI TCCGGA 1 cut(s) 1763
AoxI GGCC 6 cut(s) 12, 63, 467, 849, 871, 1699
ApeKI GCWGC 4 cut(s) 154, 204, 726, 1193
ApoI RAATTY 5 cut(s) 35, 168, 290, 1274, 1430
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 4 cut(s) 570, 929, 1410, 1572
AspLEI GCGC 2 cut(s) 1810, 1812
AspS9I GGNCC 4 cut(s) 467, 894, 1104, 1829
AsuHPI GGTGA 3 cut(s) 890, 980, 1958
AvaI CYCGRG 2 cut(s) 217, 995
AvaII GGWCC 3 cut(s) 894, 1104, 1829
AxyI CCTNAGG 2 cut(s) 41, 1944
BalI TGGCCA 1 cut(s) 873
BamHI GGATCC 1 cut(s) 983
BanII GRGCYC 2 cut(s) 996, 1684
BauI CACGAG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 5 cut(s) 33, 284, 1684, 1776, 2064
BbvI GCAGC 4 cut(s) 141, 191, 713, 1205
BccI CCATC 4 cut(s) 136, 428, 1004, 1010
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 8 cut(s) 558, 592, 635, 669, 899, 933, 986, 1020
BciT130I CCWGG 2 cut(s) 338, 875
BciVI GTATCC 1 cut(s) 797
BcoDI GTCTC 5 cut(s) 25, 435, 1737, 1859, 2026
BfaI CTAG 2 cut(s) 225, 2057
BfmI CTRYAG 2 cut(s) 717, 1335
BfrI CTTAAG 1 cut(s) 1250
BfuAI ACCTGC 1 cut(s) 1977
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 1894
BisI GCNGC 5 cut(s) 155, 158, 205, 727, 1194
BlsI GCNGC 5 cut(s) 156, 159, 206, 728, 1195
BmcAI AGTACT 1 cut(s) 1877
Bme1390I CCNGG 2 cut(s) 338, 875
Bme18I GGWCC 3 cut(s) 894, 1104, 1829
BmeRI GACNNNNNGTC 1 cut(s) 1729
BmeT110I CYCGRG 2 cut(s) 217, 995
BmgT120I GGNCC 4 cut(s) 467, 894, 1104, 1829
BmiI GGNNCC 3 cut(s) 147, 895, 985
BmrFI CCNGG 2 cut(s) 338, 875
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 1396, 1540
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 6 cut(s) 1745, 1777, 1758, 1790, 1937, 1969
Bsa29I ATCGAT 1 cut(s) 1836
BsaBI GATNNNNATC 1 cut(s) 319
BsaHI GRCGYC 2 cut(s) 18, 2035
BsaI GGTCTC 2 cut(s) 1737, 1859
BsaJI CCNNGG 7 cut(s) 336, 630, 874, 938, 996, 1956, 2066
BsaWI WCCGGW 3 cut(s) 190, 1106, 1763
BsaXI ACNNNNNCTCC 6 cut(s) 1005, 1035, 1227, 1257, 1686, 1716
Bsc4I CCNNNNNNNGG 5 cut(s) 190, 796, 1001, 1787, 1986
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 111, 1932
Bse21I CCTNAGG 2 cut(s) 41, 1944
Bse8I GATNNNNATC 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 1763
BseBI CCWGG 2 cut(s) 338, 875
BseCI ATCGAT 1 cut(s) 1836
BseDI CCNNGG 7 cut(s) 336, 630, 874, 938, 996, 1956, 2066
BseGI GGATG 2 cut(s) 640, 1300
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 5 cut(s) 190, 796, 1001, 1787, 1986
BseMII CTCAG 5 cut(s) 32, 374, 483, 1935, 2019
BseNI ACTGG 2 cut(s) 111, 1932
BsePI GCGCGC 1 cut(s) 1808
BseRI GAGGAG 1 cut(s) 1961
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 4 cut(s) 141, 191, 713, 1205
BseYI CCCAGC 1 cut(s) 544
Bsh1236I CGCG 2 cut(s) 378, 1810
Bsh1285I CGRYCG 2 cut(s) 15, 191
BshFI GGCC 6 cut(s) 14, 65, 469, 851, 873, 1701
BshVI ATCGAT 1 cut(s) 1836
BsiEI CGRYCG 2 cut(s) 15, 191
BsiHKAI GWGCWC 5 cut(s) 33, 284, 1684, 1776, 2064
BsiHKCI CYCGRG 2 cut(s) 217, 995
BsiSI CCGG 5 cut(s) 161, 173, 191, 1107, 1764
BslFI GGGAC 2 cut(s) 20, 471
BslI CCNNNNNNNGG 5 cut(s) 190, 796, 1001, 1787, 1986
BsmAI GTCTC 5 cut(s) 25, 435, 1737, 1859, 2026
BsmBI CGTCTC 2 cut(s) 25, 2026
BsmFI GGGAC 2 cut(s) 20, 471
BsmI GAATGC 3 cut(s) 218, 728, 1827
BsnI GGCC 6 cut(s) 14, 65, 469, 851, 873, 1701
Bso31I GGTCTC 2 cut(s) 1737, 1859
BsoBI CYCGRG 2 cut(s) 217, 995
Bsp1286I GDGCHC 6 cut(s) 33, 284, 996, 1684, 1776, 2064
Bsp13I TCCGGA 1 cut(s) 1763
Bsp1407I TGTACA 2 cut(s) 1044, 1210
Bsp19I CCATGG 1 cut(s) 2066
BspACI CCGC 6 cut(s) 26, 152, 158, 233, 1751, 2043
BspANI GGCC 6 cut(s) 14, 65, 469, 851, 873, 1701
BspCNI CTCAG 5 cut(s) 33, 373, 484, 1936, 2020
BspDI ATCGAT 1 cut(s) 1836
BspEI TCCGGA 1 cut(s) 1763
BspFNI CGCG 2 cut(s) 378, 1810
BspHI TCATGA 3 cut(s) 447, 507, 1219
BspLI GGNNCC 3 cut(s) 147, 895, 985
BspMAI CTGCAG 2 cut(s) 721, 1339
BspMI ACCTGC 1 cut(s) 1977
BspPI GGATC 4 cut(s) 229, 978, 991, 1795
BspQI GCTCTTC 1 cut(s) 289
BspTI CTTAAG 1 cut(s) 1250
BspTNI GGTCTC 2 cut(s) 1737, 1859
BsrFI RCCGGY 1 cut(s) 160
BsrGI TGTACA 2 cut(s) 1044, 1210
BsrI ACTGG 2 cut(s) 111, 1932
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 7 cut(s) 336, 630, 874, 938, 996, 1956, 2066
BssHII GCGCGC 1 cut(s) 1808
BssNI GRCGYC 2 cut(s) 18, 2035
BssSI CACGAG 1 cut(s) 989
BssT1I CCWWGG 3 cut(s) 938, 1956, 2066
Bst2BI CACGAG 1 cut(s) 989
Bst2UI CCWGG 2 cut(s) 338, 875
Bst4CI ACNGT 3 cut(s) 836, 1150, 1553
Bst6I CTCTTC 2 cut(s) 289, 1397
BstACI GRCGYC 2 cut(s) 18, 2035
BstAFI CTTAAG 1 cut(s) 1250
BstAUI TGTACA 2 cut(s) 1044, 1210
BstC8I GCNNGC 4 cut(s) 376, 1159, 1810, 2012
BstDEI CTNAG 5 cut(s) 41, 360, 492, 1944, 2028
BstDSI CCRYGG 2 cut(s) 630, 2066
BstF5I GGATG 2 cut(s) 640, 1300
BstFNI CGCG 2 cut(s) 378, 1810
BstHHI GCGC 2 cut(s) 1810, 1812
BstMAI GTCTC 5 cut(s) 25, 435, 1737, 1859, 2026
BstMCI CGRYCG 2 cut(s) 15, 191
BstMWI GCNNNNNNNGC 6 cut(s) 71, 213, 375, 594, 2051, 2068
BstNI CCWGG 2 cut(s) 338, 875
BstNSI RCATGY 1 cut(s) 1729
BstSCI CCNGG 2 cut(s) 336, 873
BstSFI CTRYAG 2 cut(s) 717, 1335
BstUI CGCG 2 cut(s) 378, 1810
BstV1I GCAGC 4 cut(s) 141, 191, 713, 1205
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 3 cut(s) 221, 983, 1894
BstXI CCANNNNNNTGG 1 cut(s) 1927
BstYI RGATCY 3 cut(s) 221, 983, 1894
BstZI CGGCCG 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 1836
Bsu36I CCTNAGG 2 cut(s) 41, 1944
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 6 cut(s) 14, 65, 469, 851, 873, 1701
BsuTUI ATCGAT 1 cut(s) 1836
BtgI CCRYGG 2 cut(s) 630, 2066
BtgZI GCGATG 1 cut(s) 1517
BtsCI GGATG 2 cut(s) 640, 1300
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 489, 927, 1549
BveI ACCTGC 1 cut(s) 1977
Cac8I GCNNGC 4 cut(s) 376, 1159, 1810, 2012
CaiI CAGNNNCTG 1 cut(s) 1291
CciI TCATGA 3 cut(s) 447, 507, 1219
CfoI GCGC 2 cut(s) 1810, 1812
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 4 cut(s) 467, 894, 1104, 1829
ClaI ATCGAT 1 cut(s) 1836
CseI GACGC 1 cut(s) 2043
Csp6I GTAC 4 cut(s) 1045, 1088, 1211, 1876
CviAII CATG 8 cut(s) 88, 448, 508, 934, 1220, 1726, 1813, 2067
CviQI GTAC 4 cut(s) 1045, 1088, 1211, 1876
DdeI CTNAG 5 cut(s) 41, 360, 492, 1944, 2028
DriI GACNNNNNGTC 1 cut(s) 1729
EaeI YGGCCR 3 cut(s) 12, 849, 871
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 2 cut(s) 289, 1397
Eam1105I GACNNNNNGTC 1 cut(s) 1729
EarI CTCTTC 2 cut(s) 289, 1397
EciI GGCGGA 2 cut(s) 248, 1766
Ecl136II GAGCTC 1 cut(s) 1682
EclXI CGGCCG 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 938, 1956, 2066
Eco24I GRGCYC 2 cut(s) 996, 1684
Eco31I GGTCTC 2 cut(s) 1737, 1859
Eco47I GGWCC 3 cut(s) 894, 1104, 1829
Eco52I CGGCCG 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 1682
Eco57I CTGAAG 2 cut(s) 270, 1707
Eco81I CCTNAGG 2 cut(s) 41, 1944
Eco88I CYCGRG 2 cut(s) 217, 995
EcoICRI GAGCTC 1 cut(s) 1682
EcoO109I RGGNCCY 1 cut(s) 894
EcoRI GAATTC 2 cut(s) 35, 168
EcoRII CCWGG 2 cut(s) 336, 873
EcoT14I CCWWGG 3 cut(s) 938, 1956, 2066
EcoT38I GRGCYC 2 cut(s) 996, 1684
ErhI CCWWGG 3 cut(s) 938, 1956, 2066
Esp3I CGTCTC 2 cut(s) 25, 2026
FaeI CATG 8 cut(s) 91, 451, 511, 937, 1223, 1729, 1816, 2070
FalI AAGNNNNNCTT 2 cut(s) 1680, 1712
FaqI GGGAC 2 cut(s) 20, 471
FatI CATG 8 cut(s) 87, 447, 507, 933, 1219, 1725, 1812, 2066
Fnu4HI GCNGC 5 cut(s) 155, 158, 205, 727, 1194
FokI GGATG 2 cut(s) 647, 1307
FriOI GRGCYC 2 cut(s) 996, 1684
Fsp4HI GCNGC 5 cut(s) 155, 158, 205, 727, 1194
FspBI CTAG 2 cut(s) 225, 2057
GlaI GCGC 2 cut(s) 1809, 1811
GluI GCNGC 5 cut(s) 155, 158, 205, 727, 1194
GsaI CCCAGC 1 cut(s) 548
HaeIII GGCC 6 cut(s) 14, 65, 469, 851, 873, 1701
HapII CCGG 5 cut(s) 161, 173, 191, 1107, 1764
HgaI GACGC 1 cut(s) 2043
HhaI GCGC 2 cut(s) 1810, 1812
Hin1I GRCGYC 2 cut(s) 18, 2035
Hin1II CATG 8 cut(s) 91, 451, 511, 937, 1223, 1729, 1816, 2070
Hin6I GCGC 2 cut(s) 1808, 1810
HinP1I GCGC 2 cut(s) 1808, 1810
HindIII AAGCTT 1 cut(s) 1464
HpaII CCGG 5 cut(s) 161, 173, 191, 1107, 1764
HphI GGTGA 3 cut(s) 890, 980, 1958
Hpy166II GTNNAC 1 cut(s) 1090
Hpy8I GTNNAC 1 cut(s) 1090
Hpy99I CGWCG 3 cut(s) 20, 189, 661
HpyAV CCTTC 7 cut(s) 458, 692, 800, 919, 953, 1421, 1476
HpyCH4III ACNGT 3 cut(s) 836, 1150, 1553
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 9 cut(s) 400, 719, 920, 1157, 1337, 1489, 1531, 1663, 1972
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 213, 375, 594, 2051, 2068
HpyF3I CTNAG 5 cut(s) 41, 360, 492, 1944, 2028
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 2 cut(s) 18, 2035
Hsp92II CATG 8 cut(s) 91, 451, 511, 937, 1223, 1729, 1816, 2070
HspAI GCGC 2 cut(s) 1808, 1810
Kpn2I TCCGGA 1 cut(s) 1763
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 5 cut(s) 817, 1030, 1384, 1687, 1766
Lsp1109I GCAGC 4 cut(s) 141, 191, 713, 1205
LweI GCATC 2 cut(s) 1396, 1540
MaeI CTAG 2 cut(s) 225, 2057
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 4 cut(s) 878, 1171, 1586, 1975
MfeI CAATTG 1 cut(s) 760
MflI RGATCY 3 cut(s) 221, 983, 1894
MhlI GDGCHC 6 cut(s) 33, 284, 996, 1684, 1776, 2064
MlsI TGGCCA 1 cut(s) 873
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 6 cut(s) 2, 445, 972, 1245, 1297, 1958
MmeI TCCRAC 3 cut(s) 208, 540, 672
Mox20I TGGCCA 1 cut(s) 873
MroI TCCGGA 1 cut(s) 1763
MroXI GAANNNNTTC 4 cut(s) 570, 929, 1410, 1572
MscI TGGCCA 1 cut(s) 873
MseI TTAA 4 cut(s) 827, 948, 1251, 1302
MslI CAYNNNNRTG 1 cut(s) 1908
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 154
MspCI CTTAAG 1 cut(s) 1250
MspI CCGG 5 cut(s) 161, 173, 191, 1107, 1764
MspR9I CCNGG 2 cut(s) 338, 875
MunI CAATTG 1 cut(s) 760
Mva1269I GAATGC 3 cut(s) 218, 728, 1827
MvaI CCWGG 2 cut(s) 338, 875
MvnI CGCG 2 cut(s) 378, 1810
MwoI GCNNNNNNNGC 6 cut(s) 71, 213, 375, 594, 2051, 2068
NcoI CCATGG 1 cut(s) 2066
NlaIII CATG 8 cut(s) 91, 451, 511, 937, 1223, 1729, 1816, 2070
NlaIV GGNNCC 3 cut(s) 147, 895, 985
NmeAIII GCCGAG 2 cut(s) 209, 827
NmuCI GTSAC 1 cut(s) 878
NspI RCATGY 1 cut(s) 1729
PagI TCATGA 3 cut(s) 447, 507, 1219
PaqCI CACCTGC 1 cut(s) 1977
PauI GCGCGC 1 cut(s) 1808
PciI ACATGT 1 cut(s) 1725
PciSI GCTCTTC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 1236
PctI GAATGC 3 cut(s) 218, 728, 1827
PdmI GAANNNNTTC 4 cut(s) 570, 929, 1410, 1572
PfeI GAWTC 5 cut(s) 68, 806, 1083, 1451, 1963
PflFI GACNNNGTC 1 cut(s) 2000
PkrI GCNGC 5 cut(s) 156, 159, 206, 728, 1195
PleI GAGTC 6 cut(s) 2, 445, 971, 1244, 1296, 1957
PpsI GAGTC 6 cut(s) 2, 445, 971, 1244, 1296, 1957
PpuMI RGGWCCY 1 cut(s) 894
PscI ACATGT 1 cut(s) 1725
PsiI TTATAA 2 cut(s) 558, 1580
Psp124BI GAGCTC 1 cut(s) 1684
Psp5II RGGWCCY 1 cut(s) 894
Psp6I CCWGG 2 cut(s) 336, 873
PspFI CCCAGC 1 cut(s) 544
PspGI CCWGG 2 cut(s) 336, 873
PspN4I GGNNCC 3 cut(s) 147, 895, 985
PspPI GGNCC 4 cut(s) 467, 894, 1104, 1829
PspPPI RGGWCCY 1 cut(s) 894
PstI CTGCAG 2 cut(s) 721, 1339
PstNI CAGNNNCTG 1 cut(s) 1291
PsuI RGATCY 3 cut(s) 221, 983, 1894
PsyI GACNNNGTC 1 cut(s) 2000
PteI GCGCGC 1 cut(s) 1808
RsaI GTAC 4 cut(s) 1046, 1089, 1212, 1877
RsaNI GTAC 4 cut(s) 1045, 1088, 1211, 1876
RseI CAYNNNNRTG 1 cut(s) 1908
SacI GAGCTC 1 cut(s) 1684
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 4 cut(s) 827, 948, 1251, 1302
SatI GCNGC 5 cut(s) 155, 158, 205, 727, 1194
Sau96I GGNCC 4 cut(s) 467, 894, 1104, 1829
ScaI AGTACT 1 cut(s) 1877
SchI GAGTC 6 cut(s) 2, 445, 972, 1245, 1297, 1958
ScrFI CCNGG 2 cut(s) 338, 875
SduI GDGCHC 6 cut(s) 33, 284, 996, 1684, 1776, 2064
SfaNI GCATC 2 cut(s) 1396, 1540
SfcI CTRYAG 2 cut(s) 717, 1335
SinI GGWCC 3 cut(s) 894, 1104, 1829
SmiMI CAYNNNNRTG 1 cut(s) 1908
SmlI CTYRAG 1 cut(s) 1250
SmoI CTYRAG 1 cut(s) 1250
SsiI CCGC 6 cut(s) 26, 152, 158, 233, 1751, 2043
SspMI CTAG 2 cut(s) 225, 2057
SstI GAGCTC 1 cut(s) 1684
StyD4I CCNGG 2 cut(s) 336, 873
StyI CCWWGG 3 cut(s) 938, 1956, 2066
TaaI ACNGT 3 cut(s) 836, 1150, 1553
TaiI ACGT 1 cut(s) 21
TaqI TCGA 8 cut(s) 33, 117, 313, 393, 1239, 1281, 1836, 1995
TaqII GACCGA 1 cut(s) 1883
TatI WGTACW 3 cut(s) 1044, 1210, 1875
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 5 cut(s) 68, 806, 1083, 1451, 1963
Tru1I TTAA 4 cut(s) 827, 948, 1251, 1302
Tru9I TTAA 4 cut(s) 827, 948, 1251, 1302
TscAI CASTG 3 cut(s) 496, 927, 1556
TseFI GTSAC 1 cut(s) 878
TseI GCWGC 4 cut(s) 154, 204, 726, 1193
Tsp45I GTSAC 1 cut(s) 878
TspGWI ACGGA 3 cut(s) 291, 1190, 1655
TspRI CASTG 3 cut(s) 496, 927, 1556
Tth111I GACNNNGTC 1 cut(s) 2000
Vha464I CTTAAG 1 cut(s) 1250
VpaK11BI GGWCC 3 cut(s) 894, 1104, 1829
XapI RAATTY 5 cut(s) 35, 168, 290, 1274, 1430
XceI RCATGY 1 cut(s) 1729
XmnI GAANNNNTTC 4 cut(s) 570, 929, 1410, 1572
XspI CTAG 2 cut(s) 225, 2057
ZraI GACGTC 1 cut(s) 19
ZrmI AGTACT 1 cut(s) 1877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.