MD07G1222300.v1.1

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006817
Physical Location & Seq
Reverse (-)
29921725 .. 29925712
3988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1222300.v1.1.491

Sequence Viewer

Length: 2091 bp
ATGGCGGACCCAGCCGACGTCTCGGCGGTGCTCGAGTTCCTGAGGAAAAACGGGTTTCCGGAGGCCGAATCGGCTCTCAAAGAGGATATGATTGAAAAGGGCGATCTGGGTTCTTTCGATTACGAGAAATTCATGTTTCCGATGGTTCCTCGACTGCCGCCGGTCAGAATTCCAGCGAGCTTCCGGCGGCCGGAGGTTCAGGGCGGCGGAGAATGCTCGGAGTCGAGCTCGGCGGTGGACGACGAGTTTGTGAGCGCGGGATCTTCTACTTCTAACAAGTGCTCTTCAGAATTCACAAACCCGTATGGAGTTCGATCTACATCTCCAAGCGGTTCCCAAGCTTCATCCGATAGGTTCTCTCAGTTTGGCACGGCACGCGATTACCACGATTTCGATATGCAGAATGACCTGTTCTGGCATGATGAGAAAGATGATGGAGACTTCATGACTCCTTGCTTTGAAGGGCCGGACTTCTTCGCCTGTCCCACGGAGGATAAGTTCATCATGACTTCAGATACAGACAAAGGGAACGAGAAAATGCTGGATCTGAATCATAAATCCGAAGCATTTCAACCGGAAATAAGCCTCGATTGCCTGGACAAGAGTTGTCTTACCAATGTTTCTCCGGTGGATGATACAAGCAAGGTTTATGCGACGAATTACAAGAAAAATCAGCTGGAAGAAGGCTTTGAAGAATATTGCGCAGAATGCTGCTATCCAAGTTTGAAGGAAACCGATTTCAGCAATTACCAATTAGATGTTTCGGGGGGCATCCTTCGTGTGGATGTTGATTCTGCTCCATATGACAATATAAGCAAGAGTTCTAATTACTCTGCTAAAAGGAGTTCCAAAAATGAACAGGATGAAAAGTTTAAAGTGTCCGCTGGTTTAGACGGTAAGGTTGCAGTGAAAGATTTCATGCAAAAGGGAGTTGAAGGATATGGAGTTGGAGTCGGTGAAGTAAACAAAGGATCCCACGAGCCTGAGGTTGCTGCCGATGGAGATGATGCTGCTCCCGATGAGGTTTTGATGTACAATGATGATGAGGATTATGAAGTTTTCGACTTGAGAATCATACACAGGAAGAACAGGACCGGATTTGAAGAAAGCAAGGATTTCCCAATTGTGCTAAAGACTGTAATAGCAAGCAGGTACTATATAACGGAATACCTTGGTTCGGCAGCCTTCAGTAAGGTTGTACAGGCTCATGATCTTCACACAGGAGTCGATGTGTGCCTCAAAATCATAAAGAATGATAAAGAGTTTTTCGACCAGAGTCTGGATGAGATTAAACTTCTAAAGTTTGTCAACAAAAATGACCCTGCAGATGAACACCATGTATTGCGACTTTATGACTACTTCTACCAGCAGGAGCATCTCTTCATCGTTTGTGAACTCCTTCGGGCAAACTTGTATGAATTTCAGAAGTTCACTCAAGAATCTGGTGGAGAAGCTTACTTCACATTAAGAAGGTTGCAGCTCATAACACGTCAGTGTTTAGAGGCATTGGACTACTTGCATCGCTTGGGAATTATTCACTGCGATCTAAAGCCTGAAAACATTCTTATAAAAAGTTACAGAAGATGTGAAATAAAGGTAATTGATCTTGGAAGCAGTTGCTTTCGCACGGACAACCTGTCTCTATATGTGCAATCTCGTTCTTATAGAGCTCCTGAAGTCATTCTTGGCCTCCCTTATGATGAGAAGATTGACATGTGGTCTCTGGGCTGTATATTGGCGGAGTTATGCTCCGGAGAAGTGCTCTTTCCAAATGACGGGGTTGTAATGATCCTTGCGCGTATGATTGGAATGCTTGGTCCTATCGATCTGGATATGTTAGTGAAAGGTCAGGAGACCGATAAGTACTTCACAAACGAGTTAGATCTTTACCACATAAATGAGGAAACAAGCCAACTGGAATACATAATCCCGGAGGAGTCGTCCTTGGAGAATCACCTGCAAGTAACCGACATTGGGTTCATCGACTTTGTCAAAAGCCTGCTTGAAGTCAATCCCGAGAGACGCCCAACCGCGATGGAAGCACTGGAGCACCCATGGCTTTCCTACACATACGATGCAAGTCCCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

697

Amino Acids

78.96

Weight (kDa)

4.57

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 387 - 687 5e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 388 - 586 3.1e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
AarI CACCTGC 1 cut(s) 1965
AatII GACGTC 1 cut(s) 21
Acc16I TGCGCA 1 cut(s) 703
Acc36I ACCTGC 2 cut(s) 1140, 1965
AccB7I CCANNNNNTGG 1 cut(s) 1279
AccII CGCG 4 cut(s) 257, 378, 1798, 2033
AccIII TCCGGA 2 cut(s) 58, 1751
AclWI GGATC 5 cut(s) 268, 552, 966, 979, 1783
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 4 cut(s) 128, 168, 290, 1418
AcuI CTGAAG 4 cut(s) 270, 495, 1171, 1695
AcyI GRCGYC 2 cut(s) 18, 2023
AfaI GTAC 4 cut(s) 1034, 1154, 1200, 1865
AfiI CCNNNNNNNGG 6 cut(s) 190, 781, 1177, 1279, 1775, 1974
AflIII ACRYGT 2 cut(s) 1487, 1713
AgsI TTSAA 8 cut(s) 95, 461, 572, 692, 727, 935, 1103, 2006
AhdI GACNNNNNGTC 2 cut(s) 1636, 1717
AjiI CACGTC 1 cut(s) 1490
AjnI CCWGG 1 cut(s) 594
AjuI GAANNNNNNNTTGG 2 cut(s) 1668, 1700
AleI CACNNNNGTG 1 cut(s) 1492
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 180, 228, 341, 676, 1454, 1480, 1670
AluI AGCT 7 cut(s) 180, 228, 341, 676, 1454, 1480, 1670
Alw21I GWGCWC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
Alw26I GTCTC 6 cut(s) 25, 432, 1644, 1725, 1847, 2014
AlwI GGATC 5 cut(s) 268, 552, 966, 979, 1783
AlwNI CAGNNNCTG 1 cut(s) 1279
Ama87I CYCGRG 2 cut(s) 32, 2015
Aor13HI TCCGGA 2 cut(s) 58, 1751
AoxI GGCC 4 cut(s) 63, 188, 464, 1687
ApeKI GCWGC 5 cut(s) 711, 992, 1010, 1181, 1477
ApoI RAATTY 4 cut(s) 128, 168, 290, 1418
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 5 cut(s) 567, 914, 1398, 1560, 1680
AspLEI GCGC 3 cut(s) 257, 704, 1798
AspS9I GGNCC 4 cut(s) 7, 464, 1092, 1817
AsuC2I CCSGG 1 cut(s) 1931
AsuHPI GGTGA 2 cut(s) 968, 1946
AvaI CYCGRG 2 cut(s) 32, 2015
AvaII GGWCC 3 cut(s) 7, 1092, 1817
AxyI CCTNAGG 2 cut(s) 41, 984
BamHI GGATCC 1 cut(s) 971
BanII GRGCYC 2 cut(s) 230, 1672
BauI CACGAG 1 cut(s) 977
Bbv12I GWGCWC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
BbvI GCAGC 5 cut(s) 698, 979, 997, 1193, 1489
BccI CCATC 4 cut(s) 136, 428, 992, 2029
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 1 cut(s) 596
BcnI CCSGG 1 cut(s) 1931
BcoDI GTCTC 6 cut(s) 25, 432, 1644, 1725, 1847, 2014
BfmI CTRYAG 1 cut(s) 1323
BfuAI ACCTGC 2 cut(s) 1140, 1965
BglI GCCNNNNNGGC 1 cut(s) 71
BglII AGATCT 1 cut(s) 1882
BisI GCNGC 8 cut(s) 158, 188, 205, 712, 993, 1011, 1182, 1478
BlsI GCNGC 8 cut(s) 159, 189, 206, 713, 994, 1012, 1183, 1479
BmcAI AGTACT 1 cut(s) 1865
Bme1390I CCNGG 2 cut(s) 596, 1931
Bme18I GGWCC 3 cut(s) 7, 1092, 1817
BmeRI GACNNNNNGTC 2 cut(s) 1636, 1717
BmeT110I CYCGRG 2 cut(s) 32, 2015
BmgBI CACGTC 1 cut(s) 1490
BmgT120I GGNCC 4 cut(s) 7, 464, 1092, 1817
BmiI GGNNCC 4 cut(s) 9, 147, 334, 973
BmrFI CCNGG 2 cut(s) 596, 1931
BmsI GCATC 5 cut(s) 780, 997, 1384, 1528, 2065
BoxI GACNNNNGTC 1 cut(s) 1275
BplI GAGNNNNNCTC 6 cut(s) 212, 244, 1733, 1765, 1746, 1778
BpmI CTGGAG 1 cut(s) 2066
BpuEI CTTGAG 2 cut(s) 1087, 1419
BpuMI CCSGG 1 cut(s) 1931
Bsa29I ATCGAT 1 cut(s) 1824
BsaBI GATNNNNATC 2 cut(s) 319, 549
BsaHI GRCGYC 2 cut(s) 18, 2023
BsaI GGTCTC 2 cut(s) 1725, 1847
BsaJI CCNNGG 4 cut(s) 486, 1171, 1944, 2054
BsaWI WCCGGW 5 cut(s) 58, 574, 625, 1094, 1751
BsaXI ACNNNNNCTCC 8 cut(s) 482, 512, 936, 966, 1215, 1245, 1925, 1955
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 781, 1177, 1279, 1775, 1974
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 1920, 2049
Bse21I CCTNAGG 2 cut(s) 41, 984
Bse8I GATNNNNATC 2 cut(s) 319, 549
BseAI TCCGGA 2 cut(s) 58, 1751
BseBI CCWGG 1 cut(s) 596
BseCI ATCGAT 1 cut(s) 1824
BseDI CCNNGG 4 cut(s) 486, 1171, 1944, 2054
BseGI GGATG 6 cut(s) 344, 637, 771, 790, 868, 1288
BseJI GATNNNNATC 2 cut(s) 319, 549
BseLI CCNNNNNNNGG 6 cut(s) 190, 781, 1177, 1279, 1775, 1974
BseMII CTCAG 3 cut(s) 32, 374, 975
BseNI ACTGG 2 cut(s) 1920, 2049
BseRI GAGGAG 1 cut(s) 1949
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 5 cut(s) 698, 979, 997, 1193, 1489
BseYI CCCAGC 1 cut(s) 10
Bsh1236I CGCG 4 cut(s) 257, 378, 1798, 2033
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 4 cut(s) 65, 190, 466, 1689
BshVI ATCGAT 1 cut(s) 1824
BsiEI CGRYCG 1 cut(s) 191
BsiHKAI GWGCWC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
BsiHKCI CYCGRG 2 cut(s) 32, 2015
BslFI GGGAC 2 cut(s) 468, 2067
BslI CCNNNNNNNGG 6 cut(s) 190, 781, 1177, 1279, 1775, 1974
BsmAI GTCTC 6 cut(s) 25, 432, 1644, 1725, 1847, 2014
BsmBI CGTCTC 2 cut(s) 25, 2014
BsmFI GGGAC 2 cut(s) 468, 2067
BsmI GAATGC 3 cut(s) 218, 713, 1815
BsnI GGCC 4 cut(s) 65, 190, 466, 1689
Bso31I GGTCTC 2 cut(s) 1725, 1847
BsoBI CYCGRG 2 cut(s) 32, 2015
Bsp1286I GDGCHC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
Bsp13I TCCGGA 2 cut(s) 58, 1751
Bsp1407I TGTACA 2 cut(s) 1032, 1198
Bsp19I CCATGG 1 cut(s) 2054
BspANI GGCC 4 cut(s) 65, 190, 466, 1689
BspCNI CTCAG 3 cut(s) 33, 373, 976
BspDI ATCGAT 1 cut(s) 1824
BspEI TCCGGA 2 cut(s) 58, 1751
BspFNI CGCG 4 cut(s) 257, 378, 1798, 2033
BspHI TCATGA 3 cut(s) 444, 504, 1207
BspLI GGNNCC 4 cut(s) 9, 147, 334, 973
BspMAI CTGCAG 1 cut(s) 1327
BspMI ACCTGC 2 cut(s) 1140, 1965
BspPI GGATC 5 cut(s) 268, 552, 966, 979, 1783
BspQI GCTCTTC 1 cut(s) 289
BspTNI GGTCTC 2 cut(s) 1725, 1847
BsrFI RCCGGY 1 cut(s) 160
BsrGI TGTACA 2 cut(s) 1032, 1198
BsrI ACTGG 2 cut(s) 1920, 2049
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 4 cut(s) 486, 1171, 1944, 2054
BssNI GRCGYC 2 cut(s) 18, 2023
BssSI CACGAG 1 cut(s) 977
BssT1I CCWWGG 3 cut(s) 1171, 1944, 2054
Bst2BI CACGAG 1 cut(s) 977
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 2 cut(s) 896, 1138
Bst6I CTCTTC 2 cut(s) 289, 1385
BstACI GRCGYC 2 cut(s) 18, 2023
BstAUI TGTACA 2 cut(s) 1032, 1198
BstC8I GCNNGC 4 cut(s) 178, 376, 1147, 2000
BstDEI CTNAG 3 cut(s) 41, 360, 984
BstDSI CCRYGG 2 cut(s) 486, 2054
BstF5I GGATG 6 cut(s) 344, 637, 771, 790, 868, 1288
BstFNI CGCG 4 cut(s) 257, 378, 1798, 2033
BstHHI GCGC 3 cut(s) 257, 704, 1798
BstMAI GTCTC 6 cut(s) 25, 432, 1644, 1725, 1847, 2014
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 8 cut(s) 11, 71, 213, 375, 591, 708, 2039, 2056
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 1717
BstPAI GACNNNNGTC 1 cut(s) 1275
BstSCI CCNGG 2 cut(s) 594, 1929
BstSFI CTRYAG 1 cut(s) 1323
BstUI CGCG 4 cut(s) 257, 378, 1798, 2033
BstV1I GCAGC 5 cut(s) 698, 979, 997, 1193, 1489
BstX2I RGATCY 4 cut(s) 260, 544, 971, 1882
BstYI RGATCY 4 cut(s) 260, 544, 971, 1882
BstZI CGGCCG 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 1824
Bsu36I CCTNAGG 2 cut(s) 41, 984
BsuRI GGCC 4 cut(s) 65, 190, 466, 1689
BsuTUI ATCGAT 1 cut(s) 1824
BtgI CCRYGG 2 cut(s) 486, 2054
BtgZI GCGATG 2 cut(s) 1505, 2048
BtrI CACGTC 1 cut(s) 1490
BtsCI GGATG 6 cut(s) 344, 637, 771, 790, 868, 1288
BtsI GCAGTG 2 cut(s) 912, 1537
BtsIMutI CAGTG 4 cut(s) 912, 1499, 1537, 2042
BveI ACCTGC 2 cut(s) 1140, 1965
Cac8I GCNNGC 4 cut(s) 178, 376, 1147, 2000
CaiI CAGNNNCTG 1 cut(s) 1279
CciI TCATGA 3 cut(s) 444, 504, 1207
CfoI GCGC 3 cut(s) 257, 704, 1798
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 4 cut(s) 7, 464, 1092, 1817
ClaI ATCGAT 1 cut(s) 1824
CseI GACGC 1 cut(s) 2031
Csp6I GTAC 4 cut(s) 1033, 1153, 1199, 1864
CviAII CATG 9 cut(s) 133, 419, 445, 505, 919, 1208, 1337, 1714, 2055
CviQI GTAC 4 cut(s) 1033, 1153, 1199, 1864
DdeI CTNAG 3 cut(s) 41, 360, 984
DraI TTTAAA 1 cut(s) 874
DriI GACNNNNNGTC 2 cut(s) 1636, 1717
EaeI YGGCCR 1 cut(s) 188
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 289, 1385
Eam1105I GACNNNNNGTC 2 cut(s) 1636, 1717
EarI CTCTTC 2 cut(s) 289, 1385
EciI GGCGGA 3 cut(s) 20, 222, 1754
Ecl136II GAGCTC 2 cut(s) 228, 1670
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 3 cut(s) 1171, 1944, 2054
Eco24I GRGCYC 2 cut(s) 230, 1672
Eco31I GGTCTC 2 cut(s) 1725, 1847
Eco47I GGWCC 3 cut(s) 7, 1092, 1817
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 2 cut(s) 228, 1670
Eco57I CTGAAG 4 cut(s) 270, 495, 1171, 1695
Eco81I CCTNAGG 2 cut(s) 41, 984
Eco88I CYCGRG 2 cut(s) 32, 2015
EcoICRI GAGCTC 2 cut(s) 228, 1670
EcoRI GAATTC 2 cut(s) 168, 290
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 3 cut(s) 1171, 1944, 2054
EcoT38I GRGCYC 2 cut(s) 230, 1672
ErhI CCWWGG 3 cut(s) 1171, 1944, 2054
Esp3I CGTCTC 2 cut(s) 25, 2014
FaeI CATG 9 cut(s) 136, 422, 448, 508, 922, 1211, 1340, 1717, 2058
FalI AAGNNNNNCTT 2 cut(s) 1668, 1700
FaqI GGGAC 2 cut(s) 468, 2067
FatI CATG 9 cut(s) 132, 418, 444, 504, 918, 1207, 1336, 1713, 2054
FauI CCCGC 1 cut(s) 250
FauNDI CATATG 1 cut(s) 802
Fnu4HI GCNGC 8 cut(s) 158, 188, 205, 712, 993, 1011, 1182, 1478
FokI GGATG 6 cut(s) 331, 644, 758, 797, 875, 1295
FriOI GRGCYC 2 cut(s) 230, 1672
Fsp4HI GCNGC 8 cut(s) 158, 188, 205, 712, 993, 1011, 1182, 1478
FspI TGCGCA 1 cut(s) 703
GlaI GCGC 3 cut(s) 256, 703, 1797
GluI GCNGC 8 cut(s) 158, 188, 205, 712, 993, 1011, 1182, 1478
GsaI CCCAGC 1 cut(s) 14
GsuI CTGGAG 1 cut(s) 2066
HaeIII GGCC 4 cut(s) 65, 190, 466, 1689
HgaI GACGC 1 cut(s) 2031
HhaI GCGC 3 cut(s) 257, 704, 1798
Hin1I GRCGYC 2 cut(s) 18, 2023
Hin1II CATG 9 cut(s) 136, 422, 448, 508, 922, 1211, 1340, 1717, 2058
Hin6I GCGC 3 cut(s) 255, 702, 1796
HinP1I GCGC 3 cut(s) 255, 702, 1796
HincII GTYRAC 1 cut(s) 1309
HindII GTYRAC 1 cut(s) 1309
HindIII AAGCTT 2 cut(s) 339, 1452
HphI GGTGA 2 cut(s) 968, 1946
Hpy166II GTNNAC 5 cut(s) 238, 964, 1309, 1394, 1431
Hpy188I TCNGA 9 cut(s) 141, 167, 220, 289, 349, 514, 549, 562, 1425
Hpy8I GTNNAC 5 cut(s) 238, 964, 1309, 1394, 1431
Hpy99I CGWCG 3 cut(s) 20, 245, 658
HpyAV CCTTC 8 cut(s) 455, 677, 721, 785, 929, 1195, 1409, 1464
HpyCH4III ACNGT 2 cut(s) 896, 1138
HpyCH4IV ACGT 2 cut(s) 18, 1489
HpyCH4V TGCA 9 cut(s) 400, 905, 922, 1325, 1477, 1519, 1651, 1960, 2078
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 71, 213, 375, 591, 708, 2039, 2056
HpyF3I CTNAG 3 cut(s) 41, 360, 984
HpySE526I ACGT 2 cut(s) 18, 1489
Hsp92I GRCGYC 2 cut(s) 18, 2023
Hsp92II CATG 9 cut(s) 136, 422, 448, 508, 922, 1211, 1340, 1717, 2058
HspAI GCGC 3 cut(s) 255, 702, 1796
Kpn2I TCCGGA 2 cut(s) 58, 1751
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 6 cut(s) 802, 1018, 1372, 1675, 1754, 2047
Lsp1109I GCAGC 5 cut(s) 698, 979, 997, 1193, 1489
LweI GCATC 5 cut(s) 780, 997, 1384, 1528, 2065
MaeII ACGT 2 cut(s) 18, 1489
MaeIII GTNAC 2 cut(s) 1574, 1963
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 4 cut(s) 260, 544, 971, 1882
MhlI GDGCHC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
MlyI GAGTC 6 cut(s) 230, 442, 960, 1233, 1285, 1946
MmeI TCCRAC 1 cut(s) 928
MroI TCCGGA 2 cut(s) 58, 1751
MroXI GAANNNNTTC 5 cut(s) 567, 914, 1398, 1560, 1680
MseI TTAA 3 cut(s) 873, 1290, 1466
MslI CAYNNNNRTG 2 cut(s) 1492, 1896
MspA1I CMGCKG 2 cut(s) 676, 884
MspR9I CCNGG 2 cut(s) 596, 1931
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 3 cut(s) 218, 713, 1815
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 4 cut(s) 257, 378, 1798, 2033
MwoI GCNNNNNNNGC 8 cut(s) 11, 71, 213, 375, 591, 708, 2039, 2056
NciI CCSGG 1 cut(s) 1931
NcoI CCATGG 1 cut(s) 2054
NdeI CATATG 1 cut(s) 802
NlaIII CATG 9 cut(s) 136, 422, 448, 508, 922, 1211, 1340, 1717, 2058
NlaIV GGNNCC 4 cut(s) 9, 147, 334, 973
NmeAIII GCCGAG 1 cut(s) 209
NsbI TGCGCA 1 cut(s) 703
NspI RCATGY 1 cut(s) 1717
OliI CACNNNNGTG 1 cut(s) 1492
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 3 cut(s) 444, 504, 1207
PaqCI CACCTGC 1 cut(s) 1965
PciI ACATGT 1 cut(s) 1713
PciSI GCTCTTC 1 cut(s) 289
PctI GAATGC 3 cut(s) 218, 713, 1815
PdmI GAANNNNTTC 5 cut(s) 567, 914, 1398, 1560, 1680
PfeI GAWTC 6 cut(s) 68, 550, 791, 1071, 1439, 1951
PflFI GACNNNGTC 1 cut(s) 1988
PflMI CCANNNNNTGG 1 cut(s) 1279
PfoI TCCNGGA 1 cut(s) 1929
PkrI GCNGC 8 cut(s) 159, 189, 206, 713, 994, 1012, 1183, 1479
PleI GAGTC 6 cut(s) 229, 442, 959, 1232, 1284, 1945
PpsI GAGTC 6 cut(s) 229, 442, 959, 1232, 1284, 1945
PscI ACATGT 1 cut(s) 1713
PshAI GACNNNNGTC 1 cut(s) 1275
PsiI TTATAA 1 cut(s) 1568
Psp124BI GAGCTC 2 cut(s) 230, 1672
Psp6I CCWGG 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 4 cut(s) 9, 147, 334, 973
PspPI GGNCC 4 cut(s) 7, 464, 1092, 1817
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 1327
PstNI CAGNNNCTG 1 cut(s) 1279
PsuI RGATCY 4 cut(s) 260, 544, 971, 1882
PsyI GACNNNGTC 1 cut(s) 1988
PvuII CAGCTG 1 cut(s) 676
RsaI GTAC 4 cut(s) 1034, 1154, 1200, 1865
RsaNI GTAC 4 cut(s) 1033, 1153, 1199, 1864
RseI CAYNNNNRTG 2 cut(s) 1492, 1896
SacI GAGCTC 2 cut(s) 230, 1672
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 3 cut(s) 873, 1290, 1466
SatI GCNGC 8 cut(s) 158, 188, 205, 712, 993, 1011, 1182, 1478
Sau96I GGNCC 4 cut(s) 7, 464, 1092, 1817
ScaI AGTACT 1 cut(s) 1865
SchI GAGTC 6 cut(s) 230, 442, 960, 1233, 1285, 1946
ScrFI CCNGG 2 cut(s) 596, 1931
SduI GDGCHC 6 cut(s) 33, 230, 284, 1672, 1764, 2052
SfaNI GCATC 5 cut(s) 780, 997, 1384, 1528, 2065
SfcI CTRYAG 1 cut(s) 1323
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 7, 1092, 1817
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 2 cut(s) 1492, 1896
SmlI CTYRAG 3 cut(s) 32, 1066, 1434
SmoI CTYRAG 3 cut(s) 32, 1066, 1434
SspI AATATT 1 cut(s) 698
SstI GAGCTC 2 cut(s) 230, 1672
StyD4I CCNGG 2 cut(s) 594, 1929
StyI CCWWGG 3 cut(s) 1171, 1944, 2054
TaaI ACNGT 2 cut(s) 896, 1138
TaiI ACGT 2 cut(s) 21, 1492
TaqII GACCGA 1 cut(s) 1871
TatI WGTACW 3 cut(s) 1032, 1198, 1863
TauI GCSGC 3 cut(s) 160, 190, 207
TfiI GAWTC 6 cut(s) 68, 550, 791, 1071, 1439, 1951
Tru1I TTAA 3 cut(s) 873, 1290, 1466
Tru9I TTAA 3 cut(s) 873, 1290, 1466
TscAI CASTG 4 cut(s) 912, 1499, 1544, 2049
TseI GCWGC 5 cut(s) 711, 992, 1010, 1181, 1477
TspGWI ACGGA 3 cut(s) 503, 1178, 1643
TspRI CASTG 4 cut(s) 912, 1499, 1544, 2049
Tth111I GACNNNGTC 1 cut(s) 1988
Van91I CCANNNNNTGG 1 cut(s) 1279
VpaK11BI GGWCC 3 cut(s) 7, 1092, 1817
XapI RAATTY 4 cut(s) 128, 168, 290, 1418
XceI RCATGY 1 cut(s) 1717
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 5 cut(s) 567, 914, 1398, 1560, 1680
ZraI GACGTC 1 cut(s) 19
ZrmI AGTACT 1 cut(s) 1865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.