RLG00000027057

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006817
Physical Location & Seq
Forward (+)
8034913 .. 8039576
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027057

Sequence Viewer

Length: 1755 bp
ATGGCGGTCTCGGACGTCGAGGCAGTGCTCGAGTTCTTGAAGAAAAACGGGTTTTCCGAGGCAGAGTCTGCACTGAAAGAGGACATGATTGAAAAGGGCGATCTGGGTTCTTTTGATTATGAGAAATTCTTGTTTCCGATGGTGCCTGCGCCGCCTCCGGTGAGAATTCCGGCGAGTTTTCAGAGATCGAAGGTTCCGGGTCTGGTTGAAGTCTCGAGCTCGCGATCGAGCTCCGGGGATGACGAGTTTGTGAGCGTCGGGTCTTCTAGTACTAATAGGTGCTCTTCAGAATTTACAAATCCATATGGAATTCGATCTACATCTCCAGGCAGTTCCAAGGCTTCATCTGATAGATTCTCACAATTCGGCACCGCACGCGATTACCATGATTTTGATATGCAAAATGACTTGTTCTGGCATGATGAGAAAGATGATGCAGACTTCATGACTCCTTGCTTTGACGGGGTAGACTTCTTTGGCTGTCCCACTGAGGATAAGTTCATCATGACTTCAGACACTGACAAAAAAAACGAGGACCTGCTGGGTCTGCATCATAAATCTGAAGCATTTCGATCAGAAATAAGCCTTGATTACTTGGACAAGAACTGTCTTACTAATATTTCTTCCACGGATGCCAAAGGTGTTTATGTCACAGACTACTGTCAGTTTGAAATGAAACATCAGCTTGAAGGGGGCTCTGAGGAGGAGCCTGCTGGTGAAAATTTGAAAGAAACTGATTTGGATATCTCCCATGTGAATGTTTTGGGGGTTTTTCCTTCCGTGAATAGAATAAGAAAGAGTTCTAACTACTCTCCTAAAAGGAGTTCCACAAAGGGCTGGTTGGAAAATGTAAAAGGTTCTTCTGATTTACATGGTAAGATTGCAGAGAAAGATATCAGGCCGGATGGGATCGACAGCTCTGACGTTGGAAACAGTGATGTAAATGAAGAATCCCATGATCCTGATGAAGACAGTGATGAAGTTTCTATGTACAAAACTGATGAGAATGAGTATGAAGTTTTCGATTTAAGAGTCATACACAGAAAAAACAGGACTGGATTTGAAGCAAACAAGGATCTACCTATTGTGCTAAAGACCATAGTTGCAGGCAGATACTATATAACTGAATATCTGGGTTCGGCTGCTTTTAGTAGGGTTGTACAGGCTCATGATCTTCACACAGGAGTCGATGTTTGCCTCAAGATCATAAAAAATGATAAAGATTTTTTTGATCAGAGTTTAGATGAGATTAAACTTCTTAAGTTTGTAAACAAGTATGACCCTGCAGATGAGCGCCACATTCTGCGACTTTATGACTATTTCTATCACCAGGAGCATCTCTTCATTGTTTGTGAGCTACTCAAGGCGAATTTGTACGAATTTCAGAAGTTCAGTCGAGAATCTGGTGGAGAAGCATACTTTACATTAAGAAGGTTGCAGACCATAACCCGTCAATGTTTAGAGGCATTGGAATTCTTGCATCACCTGGGAGTTATTCACTGCGATCTCAAGCCTGAGAACATTCTCATTAAAAGTTACAGAAGATGTGAAATAAAAGTCATTGATCTTGGAAGCAGTTGCTTTCAAACAGACAACTTGTGCCTATATGTACAATCTCGTTCCTACAGGGCTCCTGAAGTCATGCTTGGGCTCTCATATGACGAGAAGATTGACATGTGGTCTCTTGGATGCATCTTGGCTGAGCTATGCTCCGGAGAAGTATGTCCCTTTGTGGCTTGGTTTTCATTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

585

Amino Acids

66.38

Weight (kDa)

4.85

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 374 - 574 7.5e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 375 - 573 2.5e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 546
AccB1I GGYRCC 2 cut(s) 142, 368
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 223, 378
AccIII TCCGGA 1 cut(s) 1712
AciI CCGC 3 cut(s) 5, 152, 372
AclWI GGATC 3 cut(s) 917, 953, 1083
AcsI RAATTY 8 cut(s) 125, 165, 290, 309, 721, 1369, 1379, 1472
AcuI CTGAAG 4 cut(s) 270, 495, 582, 1656
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 5 cut(s) 271, 992, 1161, 1376, 1611
AflII CTTAAG 1 cut(s) 1259
AflIII ACRYGT 1 cut(s) 1674
AgsI TTSAA 8 cut(s) 40, 92, 209, 671, 689, 727, 1064, 1586
AhdI GACNNNNNGTC 1 cut(s) 1678
AjnI CCWGG 3 cut(s) 325, 1329, 1485
AjuI GAANNNNNNNTTGG 2 cut(s) 1629, 1661
AluBI AGCT 6 cut(s) 219, 231, 685, 918, 1357, 1705
AluI AGCT 6 cut(s) 219, 231, 685, 918, 1357, 1705
Alw21I GWGCWC 4 cut(s) 30, 221, 233, 284
Alw26I GTCTC 3 cut(s) 13, 217, 1686
AlwI GGATC 3 cut(s) 917, 953, 1083
AlwNI CAGNNNCTG 2 cut(s) 68, 518
Ama87I CYCGRG 2 cut(s) 29, 214
Aor13HI TCCGGA 1 cut(s) 1712
AoxI GGCC 1 cut(s) 899
ApeKI GCWGC 1 cut(s) 1142
ApoI RAATTY 8 cut(s) 125, 165, 290, 309, 721, 1369, 1379, 1472
Asp700I GAANNNNTTC 2 cut(s) 567, 799
AspLEI GCGC 2 cut(s) 151, 1296
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 2 cut(s) 198, 235
AsuHPI GGTGA 4 cut(s) 172, 728, 1319, 1475
AvaI CYCGRG 2 cut(s) 29, 214
AvaII GGWCC 1 cut(s) 535
BanI GGYRCC 2 cut(s) 142, 368
BanII GRGCYC 5 cut(s) 221, 233, 698, 1633, 1653
BbsI GAAGAC 2 cut(s) 255, 975
Bbv12I GWGCWC 4 cut(s) 30, 221, 233, 284
BbvI GCAGC 1 cut(s) 1129
BccI CCATC 2 cut(s) 133, 899
BciT130I CCWGG 3 cut(s) 327, 1331, 1487
BclI TGATCA 1 cut(s) 1231
BcnI CCSGG 2 cut(s) 198, 235
BcoDI GTCTC 3 cut(s) 13, 217, 1686
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 2 cut(s) 1284, 1624
BfoI RGCGCY 1 cut(s) 1297
BfrI CTTAAG 1 cut(s) 1259
BfuAI ACCTGC 1 cut(s) 546
BisI GCNGC 2 cut(s) 152, 1143
BlpI GCTNAGC 1 cut(s) 1701
BlsI GCNGC 2 cut(s) 153, 1144
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 5 cut(s) 198, 235, 327, 1331, 1487
Bme18I GGWCC 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 1678
BmeT110I CYCGRG 2 cut(s) 29, 214
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 5 cut(s) 144, 195, 370, 708, 1632
BmrFI CCNGG 5 cut(s) 198, 235, 327, 1331, 1487
BmsI GCATC 7 cut(s) 424, 559, 622, 1345, 1489, 1679, 1701
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 255, 975
BplI GAGNNNNNCTC 2 cut(s) 1694, 1726
BpmI CTGGAG 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 1701
BpuEI CTTGAG 3 cut(s) 1184, 1346, 1493
BpuMI CCSGG 2 cut(s) 198, 235
BsaBI GATNNNNATC 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 2 cut(s) 13, 1686
BsaJI CCNNGG 5 cut(s) 57, 234, 336, 627, 1486
BsaWI WCCGGW 2 cut(s) 157, 1712
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bse1I ACTGG 1 cut(s) 1060
Bse8I GATNNNNATC 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 1712
BseBI CCWGG 3 cut(s) 327, 1331, 1487
BseDI CCNNGG 5 cut(s) 57, 234, 336, 627, 1486
BseGI GGATG 4 cut(s) 244, 637, 910, 1694
BseJI GATNNNNATC 1 cut(s) 319
BseMII CTCAG 4 cut(s) 480, 690, 1506, 1692
BseNI ACTGG 1 cut(s) 1060
BseRI GAGGAG 2 cut(s) 716, 719
BseXI GCAGC 1 cut(s) 1129
BseYI CCCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 2 cut(s) 223, 378
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 901
BshNI GGYRCC 2 cut(s) 142, 368
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 4 cut(s) 30, 221, 233, 284
BsiHKCI CYCGRG 2 cut(s) 29, 214
BsiSI CCGG 6 cut(s) 158, 170, 197, 234, 902, 1713
BslFI GGGAC 2 cut(s) 468, 1710
BsmAI GTCTC 3 cut(s) 13, 217, 1686
BsmFI GGGAC 2 cut(s) 468, 1710
BsnI GGCC 1 cut(s) 901
Bso31I GGTCTC 2 cut(s) 13, 1686
BsoBI CYCGRG 2 cut(s) 29, 214
Bsp1286I GDGCHC 7 cut(s) 30, 221, 233, 284, 698, 1633, 1653
Bsp13I TCCGGA 1 cut(s) 1712
Bsp1407I TGTACA 3 cut(s) 990, 1159, 1609
Bsp1720I GCTNAGC 1 cut(s) 1701
Bsp68I TCGCGA 1 cut(s) 223
BspACI CCGC 3 cut(s) 5, 152, 372
BspANI GGCC 1 cut(s) 901
BspCNI CTCAG 4 cut(s) 481, 691, 1507, 1693
BspEI TCCGGA 1 cut(s) 1712
BspFNI CGCG 2 cut(s) 223, 378
BspHI TCATGA 3 cut(s) 444, 504, 1168
BspLI GGNNCC 5 cut(s) 144, 195, 370, 708, 1632
BspMAI CTGCAG 1 cut(s) 1288
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 3 cut(s) 917, 953, 1083
BspQI GCTCTTC 1 cut(s) 289
BspT107I GGYRCC 2 cut(s) 142, 368
BspTI CTTAAG 1 cut(s) 1259
BspTNI GGTCTC 2 cut(s) 13, 1686
BsrGI TGTACA 3 cut(s) 990, 1159, 1609
BsrI ACTGG 1 cut(s) 1060
BssECI CCNNGG 5 cut(s) 57, 234, 336, 627, 1486
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 3 cut(s) 327, 1331, 1487
Bst4CI ACNGT 4 cut(s) 608, 662, 935, 974
Bst6I CTCTTC 2 cut(s) 289, 1346
BstACI GRCGYC 1 cut(s) 15
BstAFI CTTAAG 1 cut(s) 1259
BstAPI GCANNNNNTGC 1 cut(s) 68
BstAUI TGTACA 3 cut(s) 990, 1159, 1609
BstC8I GCNNGC 5 cut(s) 147, 221, 376, 711, 1108
BstDEI CTNAG 4 cut(s) 489, 699, 1515, 1701
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 4 cut(s) 244, 637, 910, 1694
BstFNI CGCG 2 cut(s) 223, 378
BstH2I RGCGCY 1 cut(s) 1297
BstHHI GCGC 2 cut(s) 151, 1296
BstMAI GTCTC 3 cut(s) 13, 217, 1686
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 4 cut(s) 68, 151, 375, 547
BstNI CCWGG 3 cut(s) 327, 1331, 1487
BstNSI RCATGY 1 cut(s) 1678
BstPAI GACNNNNGTC 1 cut(s) 660
BstSCI CCNGG 5 cut(s) 196, 233, 325, 1329, 1485
BstSFI CTRYAG 2 cut(s) 1284, 1624
BstUI CGCG 2 cut(s) 223, 378
BstV1I GCAGC 1 cut(s) 1129
BstV2I GAAGAC 2 cut(s) 255, 975
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BsuRI GGCC 1 cut(s) 901
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 244, 637, 910, 1694
BtsI GCAGTG 2 cut(s) 30, 1498
BtsIMutI CAGTG 7 cut(s) 30, 71, 486, 516, 940, 979, 1498
BtuMI TCGCGA 1 cut(s) 223
BveI ACCTGC 1 cut(s) 546
Cac8I GCNNGC 5 cut(s) 147, 221, 376, 711, 1108
CaiI CAGNNNCTG 2 cut(s) 68, 518
CciI TCATGA 3 cut(s) 444, 504, 1168
CfoI GCGC 2 cut(s) 151, 1296
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 244
Csp6I GTAC 5 cut(s) 270, 991, 1160, 1375, 1610
CviQI GTAC 5 cut(s) 270, 991, 1160, 1375, 1610
DdeI CTNAG 4 cut(s) 489, 699, 1515, 1701
DriI GACNNNNNGTC 1 cut(s) 1678
Eam1104I CTCTTC 2 cut(s) 289, 1346
Eam1105I GACNNNNNGTC 1 cut(s) 1678
EarI CTCTTC 2 cut(s) 289, 1346
Ecl136II GAGCTC 2 cut(s) 219, 231
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 5 cut(s) 221, 233, 698, 1633, 1653
Eco31I GGTCTC 2 cut(s) 13, 1686
Eco32I GATATC 2 cut(s) 745, 895
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 2 cut(s) 219, 231
Eco57I CTGAAG 4 cut(s) 270, 495, 582, 1656
Eco88I CYCGRG 2 cut(s) 29, 214
EcoICRI GAGCTC 2 cut(s) 219, 231
EcoO109I RGGNCCY 1 cut(s) 535
EcoRI GAATTC 3 cut(s) 165, 309, 1472
EcoRII CCWGG 3 cut(s) 325, 1329, 1485
EcoRV GATATC 2 cut(s) 745, 895
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 1694
EcoT38I GRGCYC 5 cut(s) 221, 233, 698, 1633, 1653
ErhI CCWWGG 1 cut(s) 336
FalI AAGNNNNNCTT 2 cut(s) 1629, 1661
FaqI GGGAC 2 cut(s) 468, 1710
FauNDI CATATG 2 cut(s) 304, 1657
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 2 cut(s) 152, 1143
FokI GGATG 4 cut(s) 251, 644, 917, 1701
FriOI GRGCYC 5 cut(s) 221, 233, 698, 1633, 1653
Fsp4HI GCNGC 2 cut(s) 152, 1143
FspBI CTAG 1 cut(s) 267
GlaI GCGC 2 cut(s) 150, 1295
GluI GCNGC 2 cut(s) 152, 1143
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 1297
HaeIII GGCC 1 cut(s) 901
HapII CCGG 6 cut(s) 158, 170, 197, 234, 902, 1713
HgaI GACGC 1 cut(s) 244
HhaI GCGC 2 cut(s) 151, 1296
Hin1I GRCGYC 1 cut(s) 15
Hin6I GCGC 2 cut(s) 149, 1294
HinP1I GCGC 2 cut(s) 149, 1294
HinfI GANTC 7 cut(s) 65, 354, 448, 950, 1032, 1185, 1400
HpaII CCGG 6 cut(s) 158, 170, 197, 234, 902, 1713
HphI GGTGA 4 cut(s) 172, 728, 1319, 1475
Hpy166II GTNNAC 2 cut(s) 469, 1270
Hpy8I GTNNAC 2 cut(s) 469, 1270
Hpy99I CGWCG 2 cut(s) 20, 260
HpyAV CCTTC 4 cut(s) 184, 683, 786, 1425
HpyCH4III ACNGT 4 cut(s) 608, 662, 935, 974
HpyCH4IV ACGT 2 cut(s) 15, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 151, 375, 547
HpyF3I CTNAG 4 cut(s) 489, 699, 1515, 1701
HpySE526I ACGT 2 cut(s) 15, 924
Hsp92I GRCGYC 1 cut(s) 15
HspAI GCGC 2 cut(s) 149, 1294
Kpn2I TCCGGA 1 cut(s) 1712
Ksp22I TGATCA 1 cut(s) 1231
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 5 cut(s) 236, 706, 1333, 1636, 1715
Lsp1109I GCAGC 1 cut(s) 1129
LweI GCATC 7 cut(s) 424, 559, 622, 1345, 1489, 1679, 1701
MaeI CTAG 1 cut(s) 267
MaeII ACGT 2 cut(s) 15, 924
MaeIII GTNAC 2 cut(s) 649, 1535
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 7 cut(s) 30, 221, 233, 284, 698, 1633, 1653
MluCI AATT 9 cut(s) 125, 165, 290, 309, 362, 721, 1369, 1379, 1472
MlyI GAGTC 4 cut(s) 74, 442, 1041, 1194
MmeI TCCRAC 2 cut(s) 822, 907
Mph1103I ATGCAT 1 cut(s) 1694
MroI TCCGGA 1 cut(s) 1712
MroXI GAANNNNTTC 2 cut(s) 567, 799
MseI TTAA 5 cut(s) 1028, 1251, 1260, 1427, 1530
MslI CAYNNNNRTG 1 cut(s) 756
MspCI CTTAAG 1 cut(s) 1259
MspI CCGG 6 cut(s) 158, 170, 197, 234, 902, 1713
MspR9I CCNGG 5 cut(s) 198, 235, 327, 1331, 1487
MvaI CCWGG 3 cut(s) 327, 1331, 1487
MvnI CGCG 2 cut(s) 223, 378
MwoI GCNNNNNNNGC 4 cut(s) 68, 151, 375, 547
NciI CCSGG 2 cut(s) 198, 235
NdeI CATATG 2 cut(s) 304, 1657
NlaIV GGNNCC 5 cut(s) 144, 195, 370, 708, 1632
NmuCI GTSAC 1 cut(s) 649
NruI TCGCGA 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 1694
NspI RCATGY 1 cut(s) 1678
PaeR7I CTCGAG 2 cut(s) 29, 214
PagI TCATGA 3 cut(s) 444, 504, 1168
PciI ACATGT 1 cut(s) 1674
PciSI GCTCTTC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 2 cut(s) 567, 799
PfeI GAWTC 3 cut(s) 354, 950, 1400
PkrI GCNGC 2 cut(s) 153, 1144
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 4 cut(s) 73, 442, 1040, 1193
PpsI GAGTC 4 cut(s) 73, 442, 1040, 1193
PpuMI RGGWCCY 1 cut(s) 535
PscI ACATGT 1 cut(s) 1674
PshAI GACNNNNGTC 1 cut(s) 660
Psp124BI GAGCTC 2 cut(s) 221, 233
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 3 cut(s) 325, 1329, 1485
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 3 cut(s) 325, 1329, 1485
PspN4I GGNNCC 5 cut(s) 144, 195, 370, 708, 1632
PspPI GGNCC 1 cut(s) 535
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 1 cut(s) 1288
PstNI CAGNNNCTG 2 cut(s) 68, 518
PsuI RGATCY 1 cut(s) 1075
PvuI CGATCG 1 cut(s) 227
RruI TCGCGA 1 cut(s) 223
RsaI GTAC 5 cut(s) 271, 992, 1161, 1376, 1611
RsaNI GTAC 5 cut(s) 270, 991, 1160, 1375, 1610
RseI CAYNNNNRTG 1 cut(s) 756
SacI GAGCTC 2 cut(s) 221, 233
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 5 cut(s) 1028, 1251, 1260, 1427, 1530
SatI GCNGC 2 cut(s) 152, 1143
Sau96I GGNCC 1 cut(s) 535
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 4 cut(s) 74, 442, 1041, 1194
ScrFI CCNGG 5 cut(s) 198, 235, 327, 1331, 1487
SduI GDGCHC 7 cut(s) 30, 221, 233, 284, 698, 1633, 1653
SfaNI GCATC 7 cut(s) 424, 559, 622, 1345, 1489, 1679, 1701
SfcI CTRYAG 2 cut(s) 1284, 1624
Sfr274I CTCGAG 2 cut(s) 29, 214
SinI GGWCC 1 cut(s) 535
SlaI CTCGAG 2 cut(s) 29, 214
SmiMI CAYNNNNRTG 1 cut(s) 756
SmlI CTYRAG 6 cut(s) 29, 214, 1199, 1259, 1361, 1508
SmoI CTYRAG 6 cut(s) 29, 214, 1199, 1259, 1361, 1508
Sse9I AATT 9 cut(s) 125, 165, 290, 309, 362, 721, 1369, 1379, 1472
SsiI CCGC 3 cut(s) 5, 152, 372
SspI AATATT 1 cut(s) 619
SspMI CTAG 1 cut(s) 267
SstI GAGCTC 2 cut(s) 221, 233
StyD4I CCNGG 5 cut(s) 196, 233, 325, 1329, 1485
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 4 cut(s) 608, 662, 935, 974
TaiI ACGT 2 cut(s) 18, 927
TasI AATT 9 cut(s) 125, 165, 290, 309, 362, 721, 1369, 1379, 1472
TatI WGTACW 4 cut(s) 269, 990, 1159, 1609
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 3 cut(s) 354, 950, 1400
Tru1I TTAA 5 cut(s) 1028, 1251, 1260, 1427, 1530
Tru9I TTAA 5 cut(s) 1028, 1251, 1260, 1427, 1530
TscAI CASTG 7 cut(s) 30, 78, 493, 523, 940, 979, 1505
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 649
TspGWI ACGGA 2 cut(s) 644, 769
TspRI CASTG 7 cut(s) 30, 78, 493, 523, 940, 979, 1505
Vha464I CTTAAG 1 cut(s) 1259
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 8 cut(s) 125, 165, 290, 309, 721, 1369, 1379, 1472
XceI RCATGY 1 cut(s) 1678
XhoI CTCGAG 2 cut(s) 29, 214
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 2 cut(s) 567, 799
XspI CTAG 1 cut(s) 267
ZraI GACGTC 1 cut(s) 16
ZrmI AGTACT 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.