pycom01g17090

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006817
Physical Location & Seq
Reverse (-)
16476305 .. 16478000
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17090.2

Sequence Viewer

Length: 1254 bp
ATGGAGGACTCGGCCGACGTCTCGGCGGTGCTCGAATTCCTGAGAGCAAACGGGTTTTCGGAGGCCGAATCCGCTCTCAAAGAGGACATGATTGAGAAAGGCGAACTGGGTTCTTTCGATTACGAGAAGTTCTTGTTTCCGATGGTTCCTCCGCTGCCGCCGGTCAGAATTCCGGCGAGTTTCCGACGACCTGAGGTTCAGGGCGGTGGAGAATGCTCGGGATCGAGCTCGGCGGAGGACGATGAGTTTGTGAGCTTAGGGTCTTCTACTTCTAACAAGTGCTCTTCAGAATTTACAAATCCGTATGGAGTTCGATCAACATCTCCAAGCAGTTCCCAGGCTTCATCTGATAGGTTCTCTCAGTTTTGCAAGGCACGCGATTACCACGATTTCGATATGCAAAATGACCTGTTCTGGCACGATGAGAAAGATGATGGAGGAGACTTCATGACTCCTTGCTTTGAAGGGCCAGACTTCTTCGCTTGTCCCACTGAAGATAAGTTCATCATGACTTCCGATACAGACAAGGGGAACGAGAAAGTGCTGGTTCTGAATTATAAATCCGAGGCATTTCAATCGGAAATAAGCCTCGATTTCCTGGACAAGAGTTGTCTTACTGATGTTTCTCCCGTGGATGATACAAGGGAGGTTTATGCGATGAATTATTATCATTTTGATAAGAAAAATCAGTTGGAAGAAGGCTTTGAGGAACATTCTGCAGAATGCTGCTATCCAAGTTTGAAAAAAACTGATTTCAACGATTGCCAATTAGATGTTTCGGGGGGTATCCTTCGTATGGATATTGATTCTGCTCCATATCACAAGTTAAGCAATAGTTCTAATTACTCAGCCAAAAGGAGTTCAAAAAATGGCCAGGGTGAAAAGTTTATGGGGTCCTCTGGTTTAGACGATAAGGTTGCAGTGAAAGATTTCATGCCAAAGGGAGTTAAGGGATATGAAGGAGTCGGTGAAGTAAAAAAAGGATCCCACGAGCCTGAGGTTGCTGCTGATGGAGATGGTGTTGCTCCCAGTGAGATTTTGATGTACAATAATGACGAGGATTTTGAAGTTTTCGAGTTGAGAATCGTACACAGGAAGAACAGGACCGGATTTGAAGAAAGCAAGGATTTCCCTATTGTGCTAAAGACTGTAATTGCAAGCAGATACTATGTAACAGAATATCTTGGTTCAGCAGCTTTCAGTAAGGTTGTACAGGCTCATGATCGTTCACACAGGAGTCGATGTTTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

418

Amino Acids

46.55

Weight (kDa)

4.67

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
AatII GACGTC 1 cut(s) 21
AccBSI CCGCTC 1 cut(s) 74
AccII CGCG 1 cut(s) 378
AciI CCGC 6 cut(s) 26, 72, 152, 158, 204, 233
AclWI GGATC 3 cut(s) 229, 978, 991
AcoI YGGCCR 2 cut(s) 12, 871
AcsI RAATTY 3 cut(s) 35, 168, 290
AcuI CTGAAG 2 cut(s) 270, 513
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 1046, 1089, 1212
AfiI CCNNNNNNNGG 1 cut(s) 796
AgsI TTSAA 7 cut(s) 464, 575, 742, 757, 864, 1067, 1115
AjnI CCWGG 3 cut(s) 336, 597, 873
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AluBI AGCT 3 cut(s) 228, 255, 1196
AluI AGCT 3 cut(s) 228, 255, 1196
Alw21I GWGCWC 3 cut(s) 33, 230, 284
Alw26I GTCTC 2 cut(s) 25, 435
AlwI GGATC 3 cut(s) 229, 978, 991
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 4 cut(s) 12, 63, 467, 871
ApeKI GCWGC 4 cut(s) 154, 726, 1004, 1193
ApoI RAATTY 3 cut(s) 35, 168, 290
ArsI GACNNNNNNTTYG 4 cut(s) 230, 262, 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 929
AspS9I GGNCC 3 cut(s) 467, 894, 1104
AsuHPI GGTGA 2 cut(s) 890, 980
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 2 cut(s) 894, 1104
AxyI CCTNAGG 2 cut(s) 192, 996
BalI TGGCCA 1 cut(s) 873
BamHI GGATCC 1 cut(s) 983
BanII GRGCYC 1 cut(s) 230
BauI CACGAG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 3 cut(s) 33, 230, 284
BbvI GCAGC 4 cut(s) 141, 713, 991, 1205
BccI CCATC 4 cut(s) 136, 428, 1004, 1010
BcgI CGANNNNNNTGC 4 cut(s) 558, 592, 899, 933
BciT130I CCWGG 3 cut(s) 338, 599, 875
BciVI GTATCC 1 cut(s) 797
BcoDI GTCTC 2 cut(s) 25, 435
BfmI CTRYAG 1 cut(s) 717
BfuI GTATCC 1 cut(s) 797
BisI GCNGC 5 cut(s) 155, 158, 727, 1005, 1194
BlsI GCNGC 5 cut(s) 156, 159, 728, 1006, 1195
Bme1390I CCNGG 3 cut(s) 338, 599, 875
Bme18I GGWCC 2 cut(s) 894, 1104
BmeT110I CYCGRG 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 467, 894, 1104
BmiI GGNNCC 3 cut(s) 147, 895, 985
BmrFI CCNGG 3 cut(s) 338, 599, 875
BmrI ACTGGG 2 cut(s) 116, 1023
BmuI ACTGGG 2 cut(s) 116, 1023
BpiI GAAGAC 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 4 cut(s) 336, 564, 630, 874
BsaWI WCCGGW 1 cut(s) 1106
BsaXI ACNNNNNCTCC 3 cut(s) 1005, 1035, 1228
Bsc4I CCNNNNNNNGG 1 cut(s) 796
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 111, 1029
Bse21I CCTNAGG 2 cut(s) 192, 996
Bse8I GATNNNNATC 1 cut(s) 319
BseBI CCWGG 3 cut(s) 338, 599, 875
BseDI CCNNGG 4 cut(s) 336, 564, 630, 874
BseGI GGATG 1 cut(s) 640
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 796
BseMII CTCAG 5 cut(s) 32, 183, 374, 861, 987
BseNI ACTGG 2 cut(s) 111, 1029
BseRI GAGGAG 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 12
BseXI GCAGC 4 cut(s) 141, 713, 991, 1205
Bsh1236I CGCG 1 cut(s) 378
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 14, 65, 469, 873
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 3 cut(s) 33, 230, 284
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 3 cut(s) 161, 173, 1107
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 796
BsmAI GTCTC 2 cut(s) 25, 435
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 2 cut(s) 218, 728
BsnI GGCC 4 cut(s) 14, 65, 469, 873
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 3 cut(s) 33, 230, 284
Bsp1407I TGTACA 2 cut(s) 1044, 1210
Bsp143I GATC 4 cut(s) 221, 314, 983, 1222
BspACI CCGC 6 cut(s) 26, 72, 152, 158, 204, 233
BspANI GGCC 4 cut(s) 14, 65, 469, 873
BspCNI CTCAG 5 cut(s) 33, 184, 373, 860, 988
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 3 cut(s) 447, 507, 1219
BspLI GGNNCC 3 cut(s) 147, 895, 985
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 3 cut(s) 229, 978, 991
BspQI GCTCTTC 1 cut(s) 289
BsrBI CCGCTC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 160
BsrGI TGTACA 2 cut(s) 1044, 1210
BsrI ACTGG 2 cut(s) 111, 1029
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 4 cut(s) 336, 564, 630, 874
BssMI GATC 4 cut(s) 221, 314, 983, 1222
BssNI GRCGYC 1 cut(s) 18
BssSI CACGAG 1 cut(s) 989
Bst2BI CACGAG 1 cut(s) 989
Bst2UI CCWGG 3 cut(s) 338, 599, 875
Bst4CI ACNGT 1 cut(s) 1150
Bst6I CTCTTC 1 cut(s) 289
BstACI GRCGYC 1 cut(s) 18
BstAUI TGTACA 2 cut(s) 1044, 1210
BstC8I GCNNGC 2 cut(s) 376, 1159
BstDEI CTNAG 6 cut(s) 41, 192, 256, 360, 847, 996
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 1 cut(s) 640
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 4 cut(s) 224, 317, 986, 1225
BstMAI GTCTC 2 cut(s) 25, 435
BstMBI GATC 4 cut(s) 221, 314, 983, 1222
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 71, 375
BstNI CCWGG 3 cut(s) 338, 599, 875
BstSCI CCNGG 3 cut(s) 336, 597, 873
BstSFI CTRYAG 1 cut(s) 717
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 141, 713, 991, 1205
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 983
BstYI RGATCY 1 cut(s) 983
BstZI CGGCCG 1 cut(s) 12
Bsu36I CCTNAGG 2 cut(s) 192, 996
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 4 cut(s) 14, 65, 469, 873
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 671
BtsCI GGATG 1 cut(s) 640
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 489, 927, 1036
Cac8I GCNNGC 2 cut(s) 376, 1159
CciI TCATGA 3 cut(s) 447, 507, 1219
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 467, 894, 1104
Csp6I GTAC 3 cut(s) 1045, 1088, 1211
CviAII CATG 5 cut(s) 88, 448, 508, 934, 1220
CviQI GTAC 3 cut(s) 1045, 1088, 1211
DdeI CTNAG 6 cut(s) 41, 192, 256, 360, 847, 996
DpnI GATC 4 cut(s) 223, 316, 985, 1224
DpnII GATC 4 cut(s) 221, 314, 983, 1222
EaeI YGGCCR 2 cut(s) 12, 871
EagI CGGCCG 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
EciI GGCGGA 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 894, 1104
Eco52I CGGCCG 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 2 cut(s) 270, 513
Eco81I CCTNAGG 2 cut(s) 192, 996
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 894
EcoRI GAATTC 2 cut(s) 35, 168
EcoRII CCWGG 3 cut(s) 336, 597, 873
EcoT38I GRGCYC 1 cut(s) 230
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 5 cut(s) 91, 451, 511, 937, 1223
FaqI GGGAC 1 cut(s) 471
FatI CATG 5 cut(s) 87, 447, 507, 933, 1219
Fnu4HI GCNGC 5 cut(s) 155, 158, 727, 1005, 1194
FokI GGATG 1 cut(s) 647
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 5 cut(s) 155, 158, 727, 1005, 1194
GluI GCNGC 5 cut(s) 155, 158, 727, 1005, 1194
HaeIII GGCC 4 cut(s) 14, 65, 469, 873
HapII CCGG 3 cut(s) 161, 173, 1107
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 5 cut(s) 91, 451, 511, 937, 1223
HinfI GANTC 7 cut(s) 8, 68, 451, 806, 963, 1083, 1237
HpaII CCGG 3 cut(s) 161, 173, 1107
HphI GGTGA 2 cut(s) 890, 980
Hpy166II GTNNAC 2 cut(s) 1090, 1229
Hpy188III TCNNGA 5 cut(s) 40, 219, 448, 508, 1220
Hpy8I GTNNAC 2 cut(s) 1090, 1229
Hpy99I CGWCG 2 cut(s) 20, 189
HpyAV CCTTC 4 cut(s) 458, 692, 800, 953
HpyCH4III ACNGT 1 cut(s) 1150
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 5 cut(s) 369, 400, 719, 920, 1157
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 375
HpyF3I CTNAG 6 cut(s) 41, 192, 256, 360, 847, 996
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 5 cut(s) 91, 451, 511, 937, 1223
Kzo9I GATC 4 cut(s) 221, 314, 983, 1222
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 2 cut(s) 817, 1030
Lsp1109I GCAGC 4 cut(s) 141, 713, 991, 1205
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 1171
MalI GATC 4 cut(s) 223, 316, 985, 1224
MbiI CCGCTC 1 cut(s) 74
MboI GATC 4 cut(s) 221, 314, 983, 1222
MboII GAAGA 7 cut(s) 255, 276, 469, 506, 707, 1108, 1127
MflI RGATCY 1 cut(s) 983
MhlI GDGCHC 3 cut(s) 33, 230, 284
MlsI TGGCCA 1 cut(s) 873
MluCI AATT 8 cut(s) 35, 168, 290, 553, 661, 767, 841, 1152
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 4 cut(s) 2, 445, 972, 1246
MmeI TCCRAC 2 cut(s) 208, 672
Mox20I TGGCCA 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 929
MscI TGGCCA 1 cut(s) 873
MseI TTAA 3 cut(s) 827, 948, 1252
Msp20I TGGCCA 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 3 cut(s) 161, 173, 1107
MspR9I CCNGG 3 cut(s) 338, 599, 875
Mva1269I GAATGC 2 cut(s) 218, 728
MvaI CCWGG 3 cut(s) 338, 599, 875
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 2 cut(s) 71, 375
NdeII GATC 4 cut(s) 221, 314, 983, 1222
NlaIII CATG 5 cut(s) 91, 451, 511, 937, 1223
NlaIV GGNNCC 3 cut(s) 147, 895, 985
NmeAIII GCCGAG 1 cut(s) 209
PagI TCATGA 3 cut(s) 447, 507, 1219
PciSI GCTCTTC 1 cut(s) 289
PctI GAATGC 2 cut(s) 218, 728
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 3 cut(s) 68, 806, 1083
PfoI TCCNGGA 1 cut(s) 597
PkrI GCNGC 5 cut(s) 156, 159, 728, 1006, 1195
PleI GAGTC 4 cut(s) 2, 445, 971, 1245
PpsI GAGTC 4 cut(s) 2, 445, 971, 1245
PpuMI RGGWCCY 1 cut(s) 894
PsiI TTATAA 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 894
Psp6I CCWGG 3 cut(s) 336, 597, 873
PspGI CCWGG 3 cut(s) 336, 597, 873
PspN4I GGNNCC 3 cut(s) 147, 895, 985
PspPI GGNCC 3 cut(s) 467, 894, 1104
PspPPI RGGWCCY 1 cut(s) 894
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 983
RsaI GTAC 3 cut(s) 1046, 1089, 1212
RsaNI GTAC 3 cut(s) 1045, 1088, 1211
SacI GAGCTC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 3 cut(s) 827, 948, 1252
SatI GCNGC 5 cut(s) 155, 158, 727, 1005, 1194
Sau3AI GATC 4 cut(s) 221, 314, 983, 1222
Sau96I GGNCC 3 cut(s) 467, 894, 1104
SchI GAGTC 4 cut(s) 2, 445, 972, 1246
ScrFI CCNGG 3 cut(s) 338, 599, 875
SduI GDGCHC 3 cut(s) 33, 230, 284
SfcI CTRYAG 1 cut(s) 717
SinI GGWCC 2 cut(s) 894, 1104
Sse9I AATT 8 cut(s) 35, 168, 290, 553, 661, 767, 841, 1152
SsiI CCGC 6 cut(s) 26, 72, 152, 158, 204, 233
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 3 cut(s) 336, 597, 873
TaaI ACNGT 1 cut(s) 1150
TaiI ACGT 1 cut(s) 21
TaqI TCGA 8 cut(s) 33, 117, 224, 313, 393, 591, 1074, 1240
TasI AATT 8 cut(s) 35, 168, 290, 553, 661, 767, 841, 1152
TatI WGTACW 2 cut(s) 1044, 1210
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 3 cut(s) 68, 806, 1083
Tru1I TTAA 3 cut(s) 827, 948, 1252
Tru9I TTAA 3 cut(s) 827, 948, 1252
TscAI CASTG 3 cut(s) 496, 927, 1036
TseI GCWGC 4 cut(s) 154, 726, 1004, 1193
TspDTI ATGAA 6 cut(s) 333, 436, 493, 674, 922, 972
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 3 cut(s) 496, 927, 1036
VpaK11BI GGWCC 2 cut(s) 894, 1104
XapI RAATTY 3 cut(s) 35, 168, 290
XmnI GAANNNNTTC 1 cut(s) 929
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.