Rroxscaffold_4G00286670

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006817
Physical Location & Seq
Forward (+)
7679685 .. 7684074
4390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286670.1

Sequence Viewer

Length: 1833 bp
ATGGCGGTCTCGGACGTCGAGGCAGTGCTCGAATTCTTGAAGAAAAACGGGTTTTCCGCGGCAGAGTCTGCACTGAAAGAGGACATGATTGAAAAGGGCGATCTGGGTTCTTTTGATTATGAGAAATTCTTGTTTCCGATGGTGCCTGCGCCGCCTCCGGTGAGAATTCCGGCGAGTTTTCAGAGATCGAAGGTTCCGGGTCTGGTTGAAGTCTCGAGCTCGCGATCGAGCTCCGGGGATGATGAGTTTGTGAGCGTCGGTTCTTCTAGTACTAATAGGTGCTCTTCAGAATTTACAAATCCATATGGAATTCGATCTACATCTCCAGGCAGTTCCAAGGCTTCATCTGATAGATTCTCACAATTTGGCACCGCACGCGATTACCATGATTTTGATATGCAAAATGACTTGTTCTGGCATGATGAGAAAGATGATGCAGACTTCATGACTCCTTGCTTTGACGGGGTAGATTTCTTTGGCTGTCCCACTGAGGATAAGTTCATCATGACTTCAGACACTGACAAAAAAAACGAGGACCTGCTGGGTCTGCATCATAAATCTGAAGCCTTTCGATCAGAAATAAGCGTTGATTACTTGGACAAGAAATGTCTTACTAATATTTCTTCCACGGATGCCAAAGGTGTTTGTGTGACAGACTACTGTCAGTTTGAAATGAAACACCAGCTTGAAGGAGGCTCTGAGGAGGAGCCTGCTGGTGAAAAGTTGAAAGAAACTGATTTGGATATCTCCCATGGCTGGTTGGAAAATGTAAAAGGTTCTTCTGATTTACATGGTAAGATTGCAGAGAAAGATATCAGGCCGGATGGTGACAGCTCTGAAGTTGGAAACAGTGATGTAAATGAAGAATCCCATGATCCTGATGAAGACAGTGATGAAGTTTCTATGTACAAAACTGATGAGAATGAGTATGAGGCTCATGATCTTCACACAGGAGTCGATGTTTGCCTCAAGATCATAAAAAATGATAAAGATTTTTTTGATCAGAGTTTAGATGAGATTAAACTTCTTAAGTTTGTAAACAAGTATGACCCTGCAGATGAGCGCCACATTCTGCGTCTTTATGACTACTTCTATCACCAGGCAGGGAAAATGGAGCATCTCTTCATTGTTTGTGAGCTACTCAAGGCGAATTTGTACGAATTTCAGAAGTTCAGTCGAGAATCTGGTGGAGAAGCATACTTTACATTAAGAAGGTTGCAGGCCATAACCCGTCAATGTTTAGAGGCATTGGAATTCTTGCATCACCTGGGAGTTATTCACTGCGATCTCAAGCCTGAGAACATTCTCATTAAAAGTTACAGAAGATGTGAAATAAAAGTCATTGATCTTGGAAGTAGTTGCTTTCAAACAGACAACTTGTGCCTATATGTACAATCTCGTTCCTACAGGGCTCCTGAAGTCATGCTTGGGCTCTCATATGACGAGAAGATTGACATGTGGTCTCTTGGATGCATCTTGGCTGAGCTATGCTCCGGAGAAGTGCTCTTTCCAAATGATGGGATTGTGACGATCCTTGCACGTATCATTGGAATGCTTGGTCCTATCGATCTGGATATGTTAGTGAAAGGACAGGAGACAGACAAGTACTTCACAAACGAATTAGATCTCTACCACATAAATGAGGAGACAAACCAATTGGAATACATAATCCCTGAGGACTCCTCCCTGGAGCAACACCTGCAAGTAACCGATATTGGGTTCATTGACTTTGTCCAAAGCTTGCTTGAGATCAATCCCCAGAGACGCCCAAGCGCAGCGGAGGCACTAGAGCACCCCTGGCTTTCCTATTCATACGAATCGAGTTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

610

Amino Acids

69.19

Weight (kDa)

4.56

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 310 - 601 5.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 313 - 500 8.9e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1707
AatII GACGTC 1 cut(s) 18
Acc36I ACCTGC 2 cut(s) 546, 1707
AccB1I GGYRCC 2 cut(s) 142, 368
AccB7I CCANNNNNTGG 1 cut(s) 1517
AccII CGCG 3 cut(s) 59, 223, 378
AccIII TCCGGA 1 cut(s) 1493
AciI CCGC 6 cut(s) 5, 57, 59, 152, 372, 1778
AclWI GGATC 2 cut(s) 869, 1525
AcsI RAATTY 8 cut(s) 32, 125, 165, 290, 309, 1150, 1160, 1253
AcuI CTGAAG 5 cut(s) 270, 495, 582, 858, 1437
AcyI GRCGYC 2 cut(s) 15, 1765
AfaI GTAC 5 cut(s) 271, 908, 1157, 1392, 1607
AfiI CCNNNNNNNGG 3 cut(s) 756, 1517, 1716
AflII CTTAAG 1 cut(s) 1028
AflIII ACRYGT 1 cut(s) 1455
AgsI TTSAA 7 cut(s) 40, 92, 209, 671, 689, 727, 1367
AhdI GACNNNNNGTC 1 cut(s) 1459
AjnI CCWGG 5 cut(s) 325, 1098, 1266, 1686, 1796
AjuI GAANNNNNNNTTGG 2 cut(s) 1410, 1442
AluBI AGCT 7 cut(s) 219, 231, 685, 834, 1138, 1486, 1740
AluI AGCT 7 cut(s) 219, 231, 685, 834, 1138, 1486, 1740
Alw21I GWGCWC 6 cut(s) 30, 221, 233, 284, 1506, 1794
Alw26I GTCTC 6 cut(s) 13, 217, 1467, 1589, 1640, 1756
AlwI GGATC 2 cut(s) 869, 1525
AlwNI CAGNNNCTG 2 cut(s) 68, 518
Ama87I CYCGRG 1 cut(s) 214
Aor13HI TCCGGA 1 cut(s) 1493
AoxI GGCC 2 cut(s) 818, 1221
ApeKI GCWGC 1 cut(s) 1775
ApoI RAATTY 8 cut(s) 32, 125, 165, 290, 309, 1150, 1160, 1253
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 3 cut(s) 151, 1065, 1775
AspS9I GGNCC 2 cut(s) 535, 1559
AsuC2I CCSGG 2 cut(s) 198, 235
AsuHPI GGTGA 5 cut(s) 172, 728, 839, 1088, 1256
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 2 cut(s) 535, 1559
AxyI CCTNAGG 1 cut(s) 1674
BanI GGYRCC 2 cut(s) 142, 368
BanII GRGCYC 4 cut(s) 221, 233, 1414, 1434
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 6 cut(s) 30, 221, 233, 284, 1506, 1794
BbvI GCAGC 1 cut(s) 1787
BccI CCATC 3 cut(s) 133, 818, 1511
BciT130I CCWGG 5 cut(s) 327, 1100, 1268, 1688, 1798
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 198, 235
BcoDI GTCTC 6 cut(s) 13, 217, 1467, 1589, 1640, 1756
BfaI CTAG 2 cut(s) 267, 1787
BfmI CTRYAG 2 cut(s) 1053, 1405
BfoI RGCGCY 1 cut(s) 1066
BfrI CTTAAG 1 cut(s) 1028
BfuAI ACCTGC 2 cut(s) 546, 1707
BglII AGATCT 1 cut(s) 1624
BisI GCNGC 3 cut(s) 60, 152, 1776
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 3 cut(s) 61, 153, 1777
BmcAI AGTACT 2 cut(s) 271, 1607
Bme1390I CCNGG 7 cut(s) 198, 235, 327, 1100, 1268, 1688, 1798
Bme18I GGWCC 2 cut(s) 535, 1559
BmeRI GACNNNNNGTC 1 cut(s) 1459
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 535, 1559
BmiI GGNNCC 5 cut(s) 144, 195, 370, 708, 1413
BmrFI CCNGG 7 cut(s) 198, 235, 327, 1100, 1268, 1688, 1798
BmsI GCATC 7 cut(s) 424, 559, 622, 1126, 1270, 1460, 1482
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 891
BplI GAGNNNNNCTC 6 cut(s) 1475, 1507, 1488, 1520, 1667, 1699
BpmI CTGGAG 2 cut(s) 309, 1709
Bpu1102I GCTNAGC 1 cut(s) 1482
BpuEI CTTGAG 4 cut(s) 953, 1127, 1274, 1766
BpuMI CCSGG 2 cut(s) 198, 235
Bsa29I ATCGAT 1 cut(s) 1566
BsaAI YACGTR 1 cut(s) 1541
BsaBI GATNNNNATC 1 cut(s) 319
BsaHI GRCGYC 2 cut(s) 15, 1765
BsaI GGTCTC 2 cut(s) 13, 1467
BsaJI CCNNGG 8 cut(s) 57, 234, 336, 627, 751, 1267, 1686, 1796
BsaWI WCCGGW 2 cut(s) 157, 1493
BsaXI ACNNNNNCTCC 2 cut(s) 945, 975
Bsc4I CCNNNNNNNGG 3 cut(s) 756, 1517, 1716
Bse21I CCTNAGG 1 cut(s) 1674
Bse8I GATNNNNATC 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 1493
BseBI CCWGG 5 cut(s) 327, 1100, 1268, 1688, 1798
BseCI ATCGAT 1 cut(s) 1566
BseDI CCNNGG 8 cut(s) 57, 234, 336, 627, 751, 1267, 1686, 1796
BseGI GGATG 4 cut(s) 244, 637, 829, 1475
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 3 cut(s) 756, 1517, 1716
BseMII CTCAG 5 cut(s) 480, 690, 1287, 1473, 1665
BseRI GAGGAG 4 cut(s) 716, 719, 1658, 1672
BseXI GCAGC 1 cut(s) 1787
BseYI CCCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 3 cut(s) 59, 223, 378
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 820, 1223
BshNI GGYRCC 2 cut(s) 142, 368
BshVI ATCGAT 1 cut(s) 1566
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 6 cut(s) 30, 221, 233, 284, 1506, 1794
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 6 cut(s) 158, 170, 197, 234, 821, 1494
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 756, 1517, 1716
BsmAI GTCTC 6 cut(s) 13, 217, 1467, 1589, 1640, 1756
BsmBI CGTCTC 1 cut(s) 1756
BsmFI GGGAC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 1557
BsnI GGCC 2 cut(s) 820, 1223
Bso31I GGTCTC 2 cut(s) 13, 1467
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 8 cut(s) 30, 221, 233, 284, 1414, 1434, 1506, 1794
Bsp13I TCCGGA 1 cut(s) 1493
Bsp1407I TGTACA 2 cut(s) 906, 1390
Bsp1720I GCTNAGC 1 cut(s) 1482
Bsp19I CCATGG 1 cut(s) 751
Bsp68I TCGCGA 1 cut(s) 223
BspACI CCGC 6 cut(s) 5, 57, 59, 152, 372, 1778
BspANI GGCC 2 cut(s) 820, 1223
BspCNI CTCAG 5 cut(s) 481, 691, 1288, 1474, 1666
BspDI ATCGAT 1 cut(s) 1566
BspEI TCCGGA 1 cut(s) 1493
BspFNI CGCG 3 cut(s) 59, 223, 378
BspHI TCATGA 3 cut(s) 444, 504, 937
BspLI GGNNCC 5 cut(s) 144, 195, 370, 708, 1413
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 2 cut(s) 546, 1707
BspPI GGATC 2 cut(s) 869, 1525
BspQI GCTCTTC 1 cut(s) 289
BspT107I GGYRCC 2 cut(s) 142, 368
BspTI CTTAAG 1 cut(s) 1028
BspTNI GGTCTC 2 cut(s) 13, 1467
BsrGI TGTACA 2 cut(s) 906, 1390
BssECI CCNNGG 8 cut(s) 57, 234, 336, 627, 751, 1267, 1686, 1796
BssNI GRCGYC 2 cut(s) 15, 1765
BssT1I CCWWGG 2 cut(s) 336, 751
Bst2UI CCWGG 5 cut(s) 327, 1100, 1268, 1688, 1798
Bst4CI ACNGT 3 cut(s) 662, 851, 890
Bst6I CTCTTC 2 cut(s) 289, 1127
BstACI GRCGYC 2 cut(s) 15, 1765
BstAFI CTTAAG 1 cut(s) 1028
BstAPI GCANNNNNTGC 2 cut(s) 68, 1699
BstAUI TGTACA 2 cut(s) 906, 1390
BstBAI YACGTR 1 cut(s) 1541
BstC8I GCNNGC 6 cut(s) 147, 221, 376, 711, 1221, 1742
BstDEI CTNAG 5 cut(s) 489, 699, 1296, 1482, 1674
BstDSI CCRYGG 3 cut(s) 57, 627, 751
BstF5I GGATG 4 cut(s) 244, 637, 829, 1475
BstFNI CGCG 3 cut(s) 59, 223, 378
BstH2I RGCGCY 1 cut(s) 1066
BstHHI GCGC 3 cut(s) 151, 1065, 1775
BstMAI GTCTC 6 cut(s) 13, 217, 1467, 1589, 1640, 1756
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1699, 1781, 1798
BstNI CCWGG 5 cut(s) 327, 1100, 1268, 1688, 1798
BstNSI RCATGY 1 cut(s) 1459
BstPAI GACNNNNGTC 1 cut(s) 660
BstSCI CCNGG 7 cut(s) 196, 233, 325, 1098, 1266, 1686, 1796
BstSFI CTRYAG 2 cut(s) 1053, 1405
BstUI CGCG 3 cut(s) 59, 223, 378
BstV1I GCAGC 1 cut(s) 1787
BstV2I GAAGAC 1 cut(s) 891
BstX2I RGATCY 1 cut(s) 1624
BstYI RGATCY 1 cut(s) 1624
Bsu15I ATCGAT 1 cut(s) 1566
Bsu36I CCTNAGG 1 cut(s) 1674
BsuRI GGCC 2 cut(s) 820, 1223
BsuTUI ATCGAT 1 cut(s) 1566
BtgI CCRYGG 3 cut(s) 57, 627, 751
BtsCI GGATG 4 cut(s) 244, 637, 829, 1475
BtsI GCAGTG 2 cut(s) 30, 1279
BtsIMutI CAGTG 7 cut(s) 30, 71, 486, 516, 856, 895, 1279
BtuMI TCGCGA 1 cut(s) 223
BveI ACCTGC 2 cut(s) 546, 1707
Cac8I GCNNGC 6 cut(s) 147, 221, 376, 711, 1221, 1742
CaiI CAGNNNCTG 2 cut(s) 68, 518
CciI TCATGA 3 cut(s) 444, 504, 937
CfoI GCGC 3 cut(s) 151, 1065, 1775
Cfr13I GGNCC 2 cut(s) 535, 1559
Cfr42I CCGCGG 1 cut(s) 60
ClaI ATCGAT 1 cut(s) 1566
CseI GACGC 3 cut(s) 244, 1064, 1773
Csp6I GTAC 5 cut(s) 270, 907, 1156, 1391, 1606
CviQI GTAC 5 cut(s) 270, 907, 1156, 1391, 1606
DdeI CTNAG 5 cut(s) 489, 699, 1296, 1482, 1674
DriI GACNNNNNGTC 1 cut(s) 1459
Eam1104I CTCTTC 2 cut(s) 289, 1127
Eam1105I GACNNNNNGTC 1 cut(s) 1459
EarI CTCTTC 2 cut(s) 289, 1127
Ecl136II GAGCTC 2 cut(s) 219, 231
Eco130I CCWWGG 2 cut(s) 336, 751
Eco24I GRGCYC 4 cut(s) 221, 233, 1414, 1434
Eco31I GGTCTC 2 cut(s) 13, 1467
Eco32I GATATC 2 cut(s) 745, 814
Eco47I GGWCC 2 cut(s) 535, 1559
Eco53kI GAGCTC 2 cut(s) 219, 231
Eco57I CTGAAG 5 cut(s) 270, 495, 582, 858, 1437
Eco81I CCTNAGG 1 cut(s) 1674
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 2 cut(s) 219, 231
EcoO109I RGGNCCY 1 cut(s) 535
EcoRI GAATTC 4 cut(s) 32, 165, 309, 1253
EcoRII CCWGG 5 cut(s) 325, 1098, 1266, 1686, 1796
EcoRV GATATC 2 cut(s) 745, 814
EcoT14I CCWWGG 2 cut(s) 336, 751
EcoT22I ATGCAT 1 cut(s) 1475
EcoT38I GRGCYC 4 cut(s) 221, 233, 1414, 1434
ErhI CCWWGG 2 cut(s) 336, 751
Esp3I CGTCTC 1 cut(s) 1756
FalI AAGNNNNNCTT 2 cut(s) 1410, 1442
FaqI GGGAC 1 cut(s) 468
FauNDI CATATG 2 cut(s) 304, 1438
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 3 cut(s) 60, 152, 1776
FokI GGATG 4 cut(s) 251, 644, 836, 1482
FriOI GRGCYC 4 cut(s) 221, 233, 1414, 1434
Fsp4HI GCNGC 3 cut(s) 60, 152, 1776
FspBI CTAG 2 cut(s) 267, 1787
GlaI GCGC 3 cut(s) 150, 1064, 1774
GluI GCNGC 3 cut(s) 60, 152, 1776
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 2 cut(s) 309, 1709
HaeII RGCGCY 1 cut(s) 1066
HaeIII GGCC 2 cut(s) 820, 1223
HapII CCGG 6 cut(s) 158, 170, 197, 234, 821, 1494
HgaI GACGC 3 cut(s) 244, 1064, 1773
HhaI GCGC 3 cut(s) 151, 1065, 1775
Hin1I GRCGYC 2 cut(s) 15, 1765
Hin6I GCGC 3 cut(s) 149, 1063, 1773
HinP1I GCGC 3 cut(s) 149, 1063, 1773
HindIII AAGCTT 1 cut(s) 1738
HinfI GANTC 8 cut(s) 65, 354, 448, 866, 954, 1181, 1679, 1817
HpaII CCGG 6 cut(s) 158, 170, 197, 234, 821, 1494
HphI GGTGA 5 cut(s) 172, 728, 839, 1088, 1256
Hpy166II GTNNAC 1 cut(s) 1039
Hpy8I GTNNAC 1 cut(s) 1039
Hpy99I CGWCG 2 cut(s) 20, 260
HpyAV CCTTC 3 cut(s) 184, 683, 1206
HpyCH4III ACNGT 3 cut(s) 662, 851, 890
HpyCH4IV ACGT 2 cut(s) 15, 1540
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1699, 1781, 1798
HpyF3I CTNAG 5 cut(s) 489, 699, 1296, 1482, 1674
HpySE526I ACGT 2 cut(s) 15, 1540
Hsp92I GRCGYC 2 cut(s) 15, 1765
HspAI GCGC 3 cut(s) 149, 1063, 1773
Kpn2I TCCGGA 1 cut(s) 1493
Ksp22I TGATCA 1 cut(s) 1000
KspI CCGCGG 1 cut(s) 60
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 6 cut(s) 236, 706, 1114, 1417, 1496, 1690
Lsp1109I GCAGC 1 cut(s) 1787
LweI GCATC 7 cut(s) 424, 559, 622, 1126, 1270, 1460, 1482
MaeI CTAG 2 cut(s) 267, 1787
MaeII ACGT 2 cut(s) 15, 1540
MaeIII GTNAC 5 cut(s) 649, 827, 1316, 1525, 1705
MfeI CAATTG 1 cut(s) 1655
MflI RGATCY 1 cut(s) 1624
MhlI GDGCHC 8 cut(s) 30, 221, 233, 284, 1414, 1434, 1506, 1794
MlyI GAGTC 4 cut(s) 74, 442, 963, 1673
MmeI TCCRAC 2 cut(s) 741, 823
Mph1103I ATGCAT 1 cut(s) 1475
MroI TCCGGA 1 cut(s) 1493
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 4 cut(s) 1020, 1029, 1208, 1311
MslI CAYNNNNRTG 2 cut(s) 1550, 1638
MspA1I CMGCKG 2 cut(s) 59, 1778
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 6 cut(s) 158, 170, 197, 234, 821, 1494
MspR9I CCNGG 7 cut(s) 198, 235, 327, 1100, 1268, 1688, 1798
MunI CAATTG 1 cut(s) 1655
Mva1269I GAATGC 1 cut(s) 1557
MvaI CCWGG 5 cut(s) 327, 1100, 1268, 1688, 1798
MvnI CGCG 3 cut(s) 59, 223, 378
MwoI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1699, 1781, 1798
NciI CCSGG 2 cut(s) 198, 235
NcoI CCATGG 1 cut(s) 751
NdeI CATATG 2 cut(s) 304, 1438
NlaIV GGNNCC 5 cut(s) 144, 195, 370, 708, 1413
NmuCI GTSAC 3 cut(s) 649, 827, 1525
NruI TCGCGA 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 1475
NspI RCATGY 1 cut(s) 1459
PaeR7I CTCGAG 1 cut(s) 214
PagI TCATGA 3 cut(s) 444, 504, 937
PaqCI CACCTGC 1 cut(s) 1707
PciI ACATGT 1 cut(s) 1455
PciSI GCTCTTC 1 cut(s) 289
PctI GAATGC 1 cut(s) 1557
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 4 cut(s) 354, 866, 1181, 1817
PflFI GACNNNGTC 1 cut(s) 1730
PflMI CCANNNNNTGG 1 cut(s) 1517
PkrI GCNGC 3 cut(s) 61, 153, 1777
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 4 cut(s) 73, 442, 962, 1673
PpsI GAGTC 4 cut(s) 73, 442, 962, 1673
Ppu21I YACGTR 1 cut(s) 1541
PpuMI RGGWCCY 1 cut(s) 535
PscI ACATGT 1 cut(s) 1455
PshAI GACNNNNGTC 1 cut(s) 660
Psp124BI GAGCTC 2 cut(s) 221, 233
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 5 cut(s) 325, 1098, 1266, 1686, 1796
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 5 cut(s) 325, 1098, 1266, 1686, 1796
PspN4I GGNNCC 5 cut(s) 144, 195, 370, 708, 1413
PspPI GGNCC 2 cut(s) 535, 1559
PspPPI RGGWCCY 1 cut(s) 535
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 2 cut(s) 68, 518
PsuI RGATCY 1 cut(s) 1624
PsyI GACNNNGTC 1 cut(s) 1730
PvuI CGATCG 1 cut(s) 227
RruI TCGCGA 1 cut(s) 223
RsaI GTAC 5 cut(s) 271, 908, 1157, 1392, 1607
RsaNI GTAC 5 cut(s) 270, 907, 1156, 1391, 1606
RseI CAYNNNNRTG 2 cut(s) 1550, 1638
SacI GAGCTC 2 cut(s) 221, 233
SacII CCGCGG 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 4 cut(s) 1020, 1029, 1208, 1311
SatI GCNGC 3 cut(s) 60, 152, 1776
Sau96I GGNCC 2 cut(s) 535, 1559
ScaI AGTACT 2 cut(s) 271, 1607
SchI GAGTC 4 cut(s) 74, 442, 963, 1673
ScrFI CCNGG 7 cut(s) 198, 235, 327, 1100, 1268, 1688, 1798
SduI GDGCHC 8 cut(s) 30, 221, 233, 284, 1414, 1434, 1506, 1794
SfaNI GCATC 7 cut(s) 424, 559, 622, 1126, 1270, 1460, 1482
SfcI CTRYAG 2 cut(s) 1053, 1405
Sfr274I CTCGAG 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 60
SgrBI CCGCGG 1 cut(s) 60
SinI GGWCC 2 cut(s) 535, 1559
SlaI CTCGAG 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 1550, 1638
SmlI CTYRAG 6 cut(s) 214, 968, 1028, 1142, 1289, 1745
SmoI CTYRAG 6 cut(s) 214, 968, 1028, 1142, 1289, 1745
SsiI CCGC 6 cut(s) 5, 57, 59, 152, 372, 1778
SspI AATATT 1 cut(s) 619
SspMI CTAG 2 cut(s) 267, 1787
SstI GAGCTC 2 cut(s) 221, 233
StyD4I CCNGG 7 cut(s) 196, 233, 325, 1098, 1266, 1686, 1796
StyI CCWWGG 2 cut(s) 336, 751
TaaI ACNGT 3 cut(s) 662, 851, 890
TaiI ACGT 2 cut(s) 18, 1543
TatI WGTACW 4 cut(s) 269, 906, 1390, 1605
TauI GCSGC 2 cut(s) 62, 154
TfiI GAWTC 4 cut(s) 354, 866, 1181, 1817
Tru1I TTAA 4 cut(s) 1020, 1029, 1208, 1311
Tru9I TTAA 4 cut(s) 1020, 1029, 1208, 1311
TscAI CASTG 7 cut(s) 30, 78, 493, 523, 856, 895, 1286
TseFI GTSAC 3 cut(s) 649, 827, 1525
TseI GCWGC 1 cut(s) 1775
Tsp45I GTSAC 3 cut(s) 649, 827, 1525
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 7 cut(s) 30, 78, 493, 523, 856, 895, 1286
Tth111I GACNNNGTC 1 cut(s) 1730
Van91I CCANNNNNTGG 1 cut(s) 1517
Vha464I CTTAAG 1 cut(s) 1028
VpaK11BI GGWCC 2 cut(s) 535, 1559
XapI RAATTY 8 cut(s) 32, 125, 165, 290, 309, 1150, 1160, 1253
XceI RCATGY 1 cut(s) 1459
XhoI CTCGAG 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 267, 1787
ZraI GACGTC 1 cut(s) 16
ZrmI AGTACT 2 cut(s) 271, 1607
Zsp2I ATGCAT 1 cut(s) 1475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.