Rh1DG357600

Dual specificity tyrosine-phosphorylation-regulated kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006817
Physical Location & Seq
Reverse (-)
58145778 .. 58149595
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG357600.1

Sequence Viewer

Length: 2049 bp
ATGGCGGTCTCGGACGTCGAGGCAGTGCTCGAGTTCTTGAAGAAAAACGGGTTCTCCGAGGCAGAGTCTGCACTGAAAGAGGACATGATTGAAAAGGGCGATCTGAGTTCTTTTGATTATGAGAAATTCTTGTTTCCGATGGTGCCTGCGCCGCCTCCGGTGAGAATTCCGGCGAGTTTTCAGAGATCGAAGGTTCCGGGTCTGGTTGAAGTCTCGAGCTCGCGATCGAGCTCCGGGGATGATGAGTTTGTGAGCGTCGGGTCTTCTAGTACTAATAGGTGCTCTTCAGAATTTACAAATCCATATGGAATTCGATCTACATCTCCAGGCAGTTCCAAGGCTTCCTCTGATAGATTCTCACAATTTGGCACCGCACGCGATTACCATGATTTTGATATGCAAAATGACTTGTTCTGGCATGATGAGAAAGATGATGCAGACTTCATGACTCCTTGCTTTGACGGGGTAGACTTCTTTGGCTGTCCCACTGAGGACAAGTTCATCATGACTTCAGACACTGACAAAAGAAACGAGGACCTGCTGGGTCTGCATCATAAATCTGAAGCATTTCGATCAGAAATAAGCCTTGATTACTTGGACAAGAAATGTCTTACTAATATTTCTTCCACGGATGCCAAAGGTGTTTGTGTGACAGACTACTGTCAGTTTGAAATGAAACATCAGCTTGAAGGAGGCTCTGAGGAGGAGCCTGCTGGTGAAAAGTTGAAAGAAACTGATTTGGATATCTCCCATGTGAATGTTTTGGGGGGTTTTCCTTCCGTGAATAGAATAAGAAAGAGTTCTAACTACTCTCCGAAAAGGAGTTCCACAAAGGGCTGGTTGGAAAATGTAAAAGGTTCTTCTGATTTACATGGTAAGATTGCAGAGAAAGATATCAGGCCGGATGGTGACAGCTCTGAAGTTGGAAACAGTGATGTAAATGAAGAATCCCATGATCCTGATGAAGACAGTGATGAAGTTTCTATGTACAAAACTGATGAGAATGAGTATGAAGTTTTCGATTTAAGAGTCATACACAGAAAAAACAGGACTGGATTTGAAGCAAACAAGGATCTACCTATTGTGCTAAAGACCATAGTTGCAGGCAGATACTATATAACTGAATATCTGGGTTCAGCTGCTTTTAGTAGGGTTGTACAGGCTCATGATCTTCACACAGGAGTCGATGTTTGCCTCAAGATCATAAAAAATGATAAAGATTTTTTTGATCAGAGTTTAGATGAGATTAAACTTCTTAAGTTTGTAAACAAGTATGACCCTGCAGATGAGCGCCACATTCTGCGACTTTATGACTACTTCTATCACCAGGAGCATCTCTTCATTGTTTGTGAGCTACTCAAGGCGAATTTGTACGAATTTCAGAAGTTCAGTCGAGAATCTGGTGGAGAAGCATACTTTACATTAAGAAGGTTGCAGGCCATAACCCGTCAATGTTTAGAGGCATTGGAATTCTTGCATCACCTGGGAATTATTCACTGCGATCTCAAGCCTGAGAACATTCTCATTAAAAGTTACAGAAGATGTGAAATAAAAGTCATTGATCTTGGAAGCAGTTGCTTTCGAACAGACAACTTGTGCCTATATGTACAATCTCGTTCCTACAGGGCTCCTGAAGTCATGCTTGGGCTCTCATATGACGAGAAGATTGACATGTGGTCTCTTGGATGCATCTTGGCTGAGCTATGCTCCGGAGAAGTGCTCTTTCCAAATGATGGGATTGTGACGATCCTTGCACGTATCATTGGAATGCTTGGTCCTATCGATCTGGATATGTTAGTGAAAGGACAGGAGACAGACAAGTACTTCACAAACGAATTAGATCTCTACCACATAAATGAGGAGACAAACCAATTGGAATACATAATCCCTGAGGACTCCTCCCTGGAGCAACACCTGCAAGTAACCGATATTGGGTTCATGGACTTTGTCCAAAGCTTGCTTGAGATCAATCCCCAGAGACGCCCAAGCGCAGCGGAGGCACTAGAGCACCCCTGGCTTTCCTATTCATACGAATCGAGTTCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000166 GO:0000278 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005856 GO:0005886 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008289 GO:0008360 GO:0008361 GO:0009898 GO:0009987 GO:0010389 GO:0010564 GO:0010948 GO:0010972 GO:0015629 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0019897 GO:0019898 GO:0022402 GO:0022603 GO:0022604 GO:0023052 GO:0030307 GO:0030427 GO:0030554 GO:0030863 GO:0030864 GO:0031234 GO:0031567 GO:0031569 GO:0032153 GO:0032155 GO:0032465 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032878 GO:0032879 GO:0032880 GO:0032954 GO:0032956 GO:0032970 GO:0033036 GO:0033043 GO:0034613 GO:0035091 GO:0035556 GO:0035639 GO:0035839 GO:0036094 GO:0036211 GO:0040008 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044087 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0045786 GO:0045927 GO:0045930 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048638 GO:0048639 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051130 GO:0051179 GO:0051285 GO:0051286 GO:0051302 GO:0051493 GO:0051510 GO:0051512 GO:0051516 GO:0051518 GO:0051519 GO:0051641 GO:0051716 GO:0051726 GO:0060341 GO:0065007 GO:0065008 GO:0070727 GO:0070938 GO:0071342 GO:0071704 GO:0071840 GO:0071944 GO:0072395 GO:0072413 GO:0072453 GO:0072471 GO:0090066 GO:0097159 GO:0097367 GO:0098552 GO:0098562 GO:0099568 GO:0099738 GO:0120105 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901648 GO:1901981 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902412 GO:1902471 GO:1902749 GO:1902750 GO:1903047 GO:1903066 GO:1903067 GO:1903076 GO:1903077 GO:1903436 GO:1903505 GO:1903827 GO:1903828 GO:1904375 GO:1904376 GO:1905475 GO:1905476 GO:2000073 GO:2000769
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

682

Amino Acids

77.56

Weight (kDa)

4.77

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 373 - 673 3.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 374 - 572 7.1e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1923
AatII GACGTC 1 cut(s) 18
Acc36I ACCTGC 2 cut(s) 546, 1923
AccB1I GGYRCC 2 cut(s) 142, 368
AccB7I CCANNNNNTGG 1 cut(s) 1733
AccI GTMKAC 1 cut(s) 468
AccII CGCG 2 cut(s) 223, 378
AccIII TCCGGA 1 cut(s) 1709
AciI CCGC 4 cut(s) 5, 152, 372, 1994
AclWI GGATC 3 cut(s) 950, 1080, 1741
AcsI RAATTY 7 cut(s) 125, 165, 290, 309, 1366, 1376, 1469
AcuI CTGAAG 5 cut(s) 270, 495, 582, 939, 1653
AcyI GRCGYC 2 cut(s) 15, 1981
AfaI GTAC 6 cut(s) 271, 989, 1158, 1373, 1608, 1823
AfiI CCNNNNNNNGG 2 cut(s) 1733, 1932
AflII CTTAAG 1 cut(s) 1256
AflIII ACRYGT 1 cut(s) 1671
AgsI TTSAA 7 cut(s) 40, 92, 209, 671, 689, 727, 1061
AhdI GACNNNNNGTC 1 cut(s) 1675
AjnI CCWGG 5 cut(s) 325, 1326, 1482, 1902, 2012
AjuI GAANNNNNNNTTGG 2 cut(s) 1626, 1658
AluBI AGCT 8 cut(s) 219, 231, 685, 915, 1139, 1354, 1702, 1956
AluI AGCT 8 cut(s) 219, 231, 685, 915, 1139, 1354, 1702, 1956
Alw21I GWGCWC 6 cut(s) 30, 221, 233, 284, 1722, 2010
Alw26I GTCTC 6 cut(s) 13, 217, 1683, 1805, 1856, 1972
AlwI GGATC 3 cut(s) 950, 1080, 1741
AlwNI CAGNNNCTG 2 cut(s) 68, 518
Ama87I CYCGRG 2 cut(s) 29, 214
Aor13HI TCCGGA 1 cut(s) 1709
AoxI GGCC 2 cut(s) 899, 1437
ApeKI GCWGC 2 cut(s) 1139, 1991
ApoI RAATTY 7 cut(s) 125, 165, 290, 309, 1366, 1376, 1469
Asp700I GAANNNNTTC 2 cut(s) 567, 799
AspLEI GCGC 3 cut(s) 151, 1293, 1991
AspS9I GGNCC 2 cut(s) 535, 1775
AsuC2I CCSGG 2 cut(s) 198, 235
AsuHPI GGTGA 5 cut(s) 172, 728, 920, 1316, 1472
AsuII TTCGAA 1 cut(s) 1582
AvaI CYCGRG 2 cut(s) 29, 214
AvaII GGWCC 2 cut(s) 535, 1775
AxyI CCTNAGG 1 cut(s) 1890
BanI GGYRCC 2 cut(s) 142, 368
BanII GRGCYC 4 cut(s) 221, 233, 1630, 1650
BbsI GAAGAC 2 cut(s) 255, 972
Bbv12I GWGCWC 6 cut(s) 30, 221, 233, 284, 1722, 2010
BbvI GCAGC 2 cut(s) 1126, 2003
BccI CCATC 3 cut(s) 133, 899, 1727
BciT130I CCWGG 5 cut(s) 327, 1328, 1484, 1904, 2014
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 2 cut(s) 198, 235
BcoDI GTCTC 6 cut(s) 13, 217, 1683, 1805, 1856, 1972
BfaI CTAG 2 cut(s) 267, 2003
BfmI CTRYAG 2 cut(s) 1281, 1621
BfoI RGCGCY 1 cut(s) 1294
BfrI CTTAAG 1 cut(s) 1256
BfuAI ACCTGC 2 cut(s) 546, 1923
BglII AGATCT 1 cut(s) 1840
BisI GCNGC 3 cut(s) 152, 1140, 1992
BlpI GCTNAGC 1 cut(s) 1698
BlsI GCNGC 3 cut(s) 153, 1141, 1993
BmcAI AGTACT 2 cut(s) 271, 1823
Bme1390I CCNGG 7 cut(s) 198, 235, 327, 1328, 1484, 1904, 2014
Bme18I GGWCC 2 cut(s) 535, 1775
BmeRI GACNNNNNGTC 1 cut(s) 1675
BmeT110I CYCGRG 2 cut(s) 29, 214
BmgT120I GGNCC 2 cut(s) 535, 1775
BmiI GGNNCC 5 cut(s) 144, 195, 370, 708, 1629
BmrFI CCNGG 7 cut(s) 198, 235, 327, 1328, 1484, 1904, 2014
BmsI GCATC 7 cut(s) 424, 559, 622, 1342, 1486, 1676, 1698
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 255, 972
BplI GAGNNNNNCTC 6 cut(s) 1691, 1723, 1704, 1736, 1883, 1915
BpmI CTGGAG 2 cut(s) 309, 1925
Bpu1102I GCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 1582
BpuEI CTTGAG 4 cut(s) 1181, 1343, 1490, 1982
BpuMI CCSGG 2 cut(s) 198, 235
Bsa29I ATCGAT 1 cut(s) 1782
BsaAI YACGTR 1 cut(s) 1757
BsaBI GATNNNNATC 1 cut(s) 319
BsaHI GRCGYC 2 cut(s) 15, 1981
BsaI GGTCTC 2 cut(s) 13, 1683
BsaJI CCNNGG 7 cut(s) 57, 234, 336, 627, 1483, 1902, 2012
BsaWI WCCGGW 2 cut(s) 157, 1709
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 1173, 1203
Bsc4I CCNNNNNNNGG 2 cut(s) 1733, 1932
Bse1I ACTGG 1 cut(s) 1057
Bse21I CCTNAGG 1 cut(s) 1890
Bse8I GATNNNNATC 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 1709
BseBI CCWGG 5 cut(s) 327, 1328, 1484, 1904, 2014
BseCI ATCGAT 1 cut(s) 1782
BseDI CCNNGG 7 cut(s) 57, 234, 336, 627, 1483, 1902, 2012
BseGI GGATG 4 cut(s) 244, 637, 910, 1691
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 2 cut(s) 1733, 1932
BseMII CTCAG 6 cut(s) 95, 480, 690, 1503, 1689, 1881
BseNI ACTGG 1 cut(s) 1057
BseRI GAGGAG 4 cut(s) 716, 719, 1874, 1888
BseXI GCAGC 2 cut(s) 1126, 2003
BseYI CCCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 2 cut(s) 223, 378
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 901, 1439
BshNI GGYRCC 2 cut(s) 142, 368
BshVI ATCGAT 1 cut(s) 1782
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 6 cut(s) 30, 221, 233, 284, 1722, 2010
BsiHKCI CYCGRG 2 cut(s) 29, 214
BsiSI CCGG 6 cut(s) 158, 170, 197, 234, 902, 1710
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 2 cut(s) 1733, 1932
BsmAI GTCTC 6 cut(s) 13, 217, 1683, 1805, 1856, 1972
BsmBI CGTCTC 1 cut(s) 1972
BsmFI GGGAC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 1773
BsnI GGCC 2 cut(s) 901, 1439
Bso31I GGTCTC 2 cut(s) 13, 1683
BsoBI CYCGRG 2 cut(s) 29, 214
Bsp119I TTCGAA 1 cut(s) 1582
Bsp1286I GDGCHC 8 cut(s) 30, 221, 233, 284, 1630, 1650, 1722, 2010
Bsp13I TCCGGA 1 cut(s) 1709
Bsp1407I TGTACA 3 cut(s) 987, 1156, 1606
Bsp1720I GCTNAGC 1 cut(s) 1698
Bsp68I TCGCGA 1 cut(s) 223
BspACI CCGC 4 cut(s) 5, 152, 372, 1994
BspANI GGCC 2 cut(s) 901, 1439
BspCNI CTCAG 6 cut(s) 96, 481, 691, 1504, 1690, 1882
BspDI ATCGAT 1 cut(s) 1782
BspEI TCCGGA 1 cut(s) 1709
BspFNI CGCG 2 cut(s) 223, 378
BspHI TCATGA 3 cut(s) 444, 504, 1165
BspLI GGNNCC 5 cut(s) 144, 195, 370, 708, 1629
BspMAI CTGCAG 1 cut(s) 1285
BspMI ACCTGC 2 cut(s) 546, 1923
BspPI GGATC 3 cut(s) 950, 1080, 1741
BspQI GCTCTTC 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 1582
BspT107I GGYRCC 2 cut(s) 142, 368
BspTI CTTAAG 1 cut(s) 1256
BspTNI GGTCTC 2 cut(s) 13, 1683
BsrGI TGTACA 3 cut(s) 987, 1156, 1606
BsrI ACTGG 1 cut(s) 1057
BssECI CCNNGG 7 cut(s) 57, 234, 336, 627, 1483, 1902, 2012
BssNI GRCGYC 2 cut(s) 15, 1981
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 5 cut(s) 327, 1328, 1484, 1904, 2014
Bst4CI ACNGT 3 cut(s) 662, 932, 971
Bst6I CTCTTC 2 cut(s) 289, 1343
BstACI GRCGYC 2 cut(s) 15, 1981
BstAFI CTTAAG 1 cut(s) 1256
BstAPI GCANNNNNTGC 2 cut(s) 68, 1915
BstAUI TGTACA 3 cut(s) 987, 1156, 1606
BstBAI YACGTR 1 cut(s) 1757
BstBI TTCGAA 1 cut(s) 1582
BstC8I GCNNGC 7 cut(s) 147, 221, 376, 711, 1105, 1437, 1958
BstDEI CTNAG 6 cut(s) 104, 489, 699, 1512, 1698, 1890
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 4 cut(s) 244, 637, 910, 1691
BstFNI CGCG 2 cut(s) 223, 378
BstH2I RGCGCY 1 cut(s) 1294
BstHHI GCGC 3 cut(s) 151, 1293, 1991
BstMAI GTCTC 6 cut(s) 13, 217, 1683, 1805, 1856, 1972
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1915, 1997, 2014
BstNI CCWGG 5 cut(s) 327, 1328, 1484, 1904, 2014
BstNSI RCATGY 1 cut(s) 1675
BstPAI GACNNNNGTC 1 cut(s) 660
BstSCI CCNGG 7 cut(s) 196, 233, 325, 1326, 1482, 1902, 2012
BstSFI CTRYAG 2 cut(s) 1281, 1621
BstUI CGCG 2 cut(s) 223, 378
BstV1I GCAGC 2 cut(s) 1126, 2003
BstV2I GAAGAC 2 cut(s) 255, 972
BstX2I RGATCY 2 cut(s) 1072, 1840
BstYI RGATCY 2 cut(s) 1072, 1840
Bsu15I ATCGAT 1 cut(s) 1782
Bsu36I CCTNAGG 1 cut(s) 1890
BsuRI GGCC 2 cut(s) 901, 1439
BsuTUI ATCGAT 1 cut(s) 1782
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 244, 637, 910, 1691
BtsI GCAGTG 2 cut(s) 30, 1495
BtsIMutI CAGTG 7 cut(s) 30, 71, 486, 516, 937, 976, 1495
BtuMI TCGCGA 1 cut(s) 223
BveI ACCTGC 2 cut(s) 546, 1923
Cac8I GCNNGC 7 cut(s) 147, 221, 376, 711, 1105, 1437, 1958
CaiI CAGNNNCTG 2 cut(s) 68, 518
CciI TCATGA 3 cut(s) 444, 504, 1165
CfoI GCGC 3 cut(s) 151, 1293, 1991
Cfr13I GGNCC 2 cut(s) 535, 1775
ClaI ATCGAT 1 cut(s) 1782
CseI GACGC 2 cut(s) 244, 1989
Csp6I GTAC 6 cut(s) 270, 988, 1157, 1372, 1607, 1822
CviQI GTAC 6 cut(s) 270, 988, 1157, 1372, 1607, 1822
DdeI CTNAG 6 cut(s) 104, 489, 699, 1512, 1698, 1890
DriI GACNNNNNGTC 1 cut(s) 1675
Eam1104I CTCTTC 2 cut(s) 289, 1343
Eam1105I GACNNNNNGTC 1 cut(s) 1675
EarI CTCTTC 2 cut(s) 289, 1343
Ecl136II GAGCTC 2 cut(s) 219, 231
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 4 cut(s) 221, 233, 1630, 1650
Eco31I GGTCTC 2 cut(s) 13, 1683
Eco32I GATATC 2 cut(s) 745, 895
Eco47I GGWCC 2 cut(s) 535, 1775
Eco53kI GAGCTC 2 cut(s) 219, 231
Eco57I CTGAAG 5 cut(s) 270, 495, 582, 939, 1653
Eco81I CCTNAGG 1 cut(s) 1890
Eco88I CYCGRG 2 cut(s) 29, 214
EcoICRI GAGCTC 2 cut(s) 219, 231
EcoO109I RGGNCCY 1 cut(s) 535
EcoRI GAATTC 3 cut(s) 165, 309, 1469
EcoRII CCWGG 5 cut(s) 325, 1326, 1482, 1902, 2012
EcoRV GATATC 2 cut(s) 745, 895
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 1691
EcoT38I GRGCYC 4 cut(s) 221, 233, 1630, 1650
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 1972
FalI AAGNNNNNCTT 2 cut(s) 1626, 1658
FaqI GGGAC 1 cut(s) 468
FauNDI CATATG 2 cut(s) 304, 1654
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 152, 1140, 1992
FokI GGATG 4 cut(s) 251, 644, 917, 1698
FriOI GRGCYC 4 cut(s) 221, 233, 1630, 1650
Fsp4HI GCNGC 3 cut(s) 152, 1140, 1992
FspBI CTAG 2 cut(s) 267, 2003
GlaI GCGC 3 cut(s) 150, 1292, 1990
GluI GCNGC 3 cut(s) 152, 1140, 1992
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 2 cut(s) 309, 1925
HaeII RGCGCY 1 cut(s) 1294
HaeIII GGCC 2 cut(s) 901, 1439
HapII CCGG 6 cut(s) 158, 170, 197, 234, 902, 1710
HgaI GACGC 2 cut(s) 244, 1989
HhaI GCGC 3 cut(s) 151, 1293, 1991
Hin1I GRCGYC 2 cut(s) 15, 1981
Hin6I GCGC 3 cut(s) 149, 1291, 1989
HinP1I GCGC 3 cut(s) 149, 1291, 1989
HindIII AAGCTT 1 cut(s) 1954
HinfI GANTC 9 cut(s) 65, 354, 448, 947, 1029, 1182, 1397, 1895, 2033
HpaII CCGG 6 cut(s) 158, 170, 197, 234, 902, 1710
HphI GGTGA 5 cut(s) 172, 728, 920, 1316, 1472
Hpy166II GTNNAC 2 cut(s) 469, 1267
Hpy8I GTNNAC 2 cut(s) 469, 1267
Hpy99I CGWCG 2 cut(s) 20, 260
HpyAV CCTTC 4 cut(s) 184, 683, 786, 1422
HpyCH4III ACNGT 3 cut(s) 662, 932, 971
HpyCH4IV ACGT 2 cut(s) 15, 1756
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1915, 1997, 2014
HpyF3I CTNAG 6 cut(s) 104, 489, 699, 1512, 1698, 1890
HpySE526I ACGT 2 cut(s) 15, 1756
Hsp92I GRCGYC 2 cut(s) 15, 1981
HspAI GCGC 3 cut(s) 149, 1291, 1989
Kpn2I TCCGGA 1 cut(s) 1709
Ksp22I TGATCA 1 cut(s) 1228
LguI GCTCTTC 1 cut(s) 289
LmnI GCTCC 6 cut(s) 236, 706, 1330, 1633, 1712, 1906
Lsp1109I GCAGC 2 cut(s) 1126, 2003
LweI GCATC 7 cut(s) 424, 559, 622, 1342, 1486, 1676, 1698
MaeI CTAG 2 cut(s) 267, 2003
MaeII ACGT 2 cut(s) 15, 1756
MaeIII GTNAC 5 cut(s) 649, 908, 1532, 1741, 1921
MfeI CAATTG 1 cut(s) 1871
MflI RGATCY 2 cut(s) 1072, 1840
MhlI GDGCHC 8 cut(s) 30, 221, 233, 284, 1630, 1650, 1722, 2010
MlyI GAGTC 5 cut(s) 74, 442, 1038, 1191, 1889
MmeI TCCRAC 2 cut(s) 822, 904
Mph1103I ATGCAT 1 cut(s) 1691
MroI TCCGGA 1 cut(s) 1709
MroXI GAANNNNTTC 2 cut(s) 567, 799
MseI TTAA 5 cut(s) 1025, 1248, 1257, 1424, 1527
MslI CAYNNNNRTG 3 cut(s) 756, 1766, 1854
MspA1I CMGCKG 2 cut(s) 1139, 1994
MspCI CTTAAG 1 cut(s) 1256
MspI CCGG 6 cut(s) 158, 170, 197, 234, 902, 1710
MspR9I CCNGG 7 cut(s) 198, 235, 327, 1328, 1484, 1904, 2014
MunI CAATTG 1 cut(s) 1871
Mva1269I GAATGC 1 cut(s) 1773
MvaI CCWGG 5 cut(s) 327, 1328, 1484, 1904, 2014
MvnI CGCG 2 cut(s) 223, 378
MwoI GCNNNNNNNGC 7 cut(s) 68, 151, 375, 547, 1915, 1997, 2014
NciI CCSGG 2 cut(s) 198, 235
NdeI CATATG 2 cut(s) 304, 1654
NlaIV GGNNCC 5 cut(s) 144, 195, 370, 708, 1629
NmuCI GTSAC 3 cut(s) 649, 908, 1741
NruI TCGCGA 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 1691
NspI RCATGY 1 cut(s) 1675
NspV TTCGAA 1 cut(s) 1582
PaeR7I CTCGAG 2 cut(s) 29, 214
PagI TCATGA 3 cut(s) 444, 504, 1165
PaqCI CACCTGC 1 cut(s) 1923
PciI ACATGT 1 cut(s) 1671
PciSI GCTCTTC 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 1773
PdmI GAANNNNTTC 2 cut(s) 567, 799
PfeI GAWTC 4 cut(s) 354, 947, 1397, 2033
PflFI GACNNNGTC 1 cut(s) 1946
PflMI CCANNNNNTGG 1 cut(s) 1733
PkrI GCNGC 3 cut(s) 153, 1141, 1993
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 5 cut(s) 73, 442, 1037, 1190, 1889
PpsI GAGTC 5 cut(s) 73, 442, 1037, 1190, 1889
Ppu21I YACGTR 1 cut(s) 1757
PpuMI RGGWCCY 1 cut(s) 535
PscI ACATGT 1 cut(s) 1671
PshAI GACNNNNGTC 1 cut(s) 660
Psp124BI GAGCTC 2 cut(s) 221, 233
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 5 cut(s) 325, 1326, 1482, 1902, 2012
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 5 cut(s) 325, 1326, 1482, 1902, 2012
PspN4I GGNNCC 5 cut(s) 144, 195, 370, 708, 1629
PspPI GGNCC 2 cut(s) 535, 1775
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 29
PstI CTGCAG 1 cut(s) 1285
PstNI CAGNNNCTG 2 cut(s) 68, 518
PsuI RGATCY 2 cut(s) 1072, 1840
PsyI GACNNNGTC 1 cut(s) 1946
PvuI CGATCG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 1139
RruI TCGCGA 1 cut(s) 223
RsaI GTAC 6 cut(s) 271, 989, 1158, 1373, 1608, 1823
RsaNI GTAC 6 cut(s) 270, 988, 1157, 1372, 1607, 1822
RseI CAYNNNNRTG 3 cut(s) 756, 1766, 1854
SacI GAGCTC 2 cut(s) 221, 233
SapI GCTCTTC 1 cut(s) 289
SaqAI TTAA 5 cut(s) 1025, 1248, 1257, 1424, 1527
SatI GCNGC 3 cut(s) 152, 1140, 1992
Sau96I GGNCC 2 cut(s) 535, 1775
ScaI AGTACT 2 cut(s) 271, 1823
SchI GAGTC 5 cut(s) 74, 442, 1038, 1191, 1889
ScrFI CCNGG 7 cut(s) 198, 235, 327, 1328, 1484, 1904, 2014
SduI GDGCHC 8 cut(s) 30, 221, 233, 284, 1630, 1650, 1722, 2010
SfaNI GCATC 7 cut(s) 424, 559, 622, 1342, 1486, 1676, 1698
SfcI CTRYAG 2 cut(s) 1281, 1621
Sfr274I CTCGAG 2 cut(s) 29, 214
SfuI TTCGAA 1 cut(s) 1582
SinI GGWCC 2 cut(s) 535, 1775
SlaI CTCGAG 2 cut(s) 29, 214
SmiMI CAYNNNNRTG 3 cut(s) 756, 1766, 1854
SmlI CTYRAG 7 cut(s) 29, 214, 1196, 1256, 1358, 1505, 1961
SmoI CTYRAG 7 cut(s) 29, 214, 1196, 1256, 1358, 1505, 1961
SsiI CCGC 4 cut(s) 5, 152, 372, 1994
SspI AATATT 1 cut(s) 619
SspMI CTAG 2 cut(s) 267, 2003
SstI GAGCTC 2 cut(s) 221, 233
StyD4I CCNGG 7 cut(s) 196, 233, 325, 1326, 1482, 1902, 2012
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 3 cut(s) 662, 932, 971
TaiI ACGT 2 cut(s) 18, 1759
TatI WGTACW 5 cut(s) 269, 987, 1156, 1606, 1821
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 4 cut(s) 354, 947, 1397, 2033
Tru1I TTAA 5 cut(s) 1025, 1248, 1257, 1424, 1527
Tru9I TTAA 5 cut(s) 1025, 1248, 1257, 1424, 1527
TscAI CASTG 7 cut(s) 30, 78, 493, 523, 937, 976, 1502
TseFI GTSAC 3 cut(s) 649, 908, 1741
TseI GCWGC 2 cut(s) 1139, 1991
Tsp45I GTSAC 3 cut(s) 649, 908, 1741
TspGWI ACGGA 2 cut(s) 644, 769
TspRI CASTG 7 cut(s) 30, 78, 493, 523, 937, 976, 1502
Tth111I GACNNNGTC 1 cut(s) 1946
Van91I CCANNNNNTGG 1 cut(s) 1733
Vha464I CTTAAG 1 cut(s) 1256
VpaK11BI GGWCC 2 cut(s) 535, 1775
XapI RAATTY 7 cut(s) 125, 165, 290, 309, 1366, 1376, 1469
XceI RCATGY 1 cut(s) 1675
XhoI CTCGAG 2 cut(s) 29, 214
XmiI GTMKAC 1 cut(s) 468
XmnI GAANNNNTTC 2 cut(s) 567, 799
XspI CTAG 2 cut(s) 267, 2003
ZraI GACGTC 1 cut(s) 16
ZrmI AGTACT 2 cut(s) 271, 1823
Zsp2I ATGCAT 1 cut(s) 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.