FvH4_7g25011

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015465
Physical Location & Seq
Reverse (-)
19178407 .. 19179569
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25011.t1

Sequence Viewer

Length: 1077 bp
ATGTCATTGCGGACGTATGTGACTTGGGTTGAGATGATTAGTGGGTTTGCGAGGACTGGAGATACTGTTAGTGCTAGGAGGTTGTTTGATCAGGTTCCACCGGAGTTGAAGAATGTGATTACGTGGACTGTGATGGTTGATGGATATTGTAGCAATGGGCAGATGGAGATGGCAAGGGAACTTTTCGAAGCAATGCCCCAGCGCAACTTCTTTGTTTGGTCGTCAATGATTTCTGGGTATTTCAAGATAGGTGATGTTGAGGAGGCTAAGACGATATTTGATAGAATCCCAGTCCGGAACTTGGTAAATTGGAATTCGATGATGTCTGGATATGCGCAAAATGGATTTTGTGAGGAAGCTTTGAAAGCATTTGAGAACATGCAAGCTGAAGGCTTTGAGCCAGATGAGTTTACTGTTGTGAGTGTCTTATCTGCTTGTTCTCAGTCTGGGCTATTAGATGTTGGCAAGGATATACATAACATGTTAAGTCATAACAGGCTTAATCAGATTGTTCACAATGCTCTTGTAGACATGTATGCAAAATGTGGCGACTTGATCAATGCAAGGTTGATTTTTGAGGAGATGACTGAGAGGAACAGTGCTTGTTGGAATGCTATGATTTCAAGCATGGCTATTCATGGACAGTGCAAGGAGGCTCTTGAATTGTTTAGCAGAATGGGGGATTCAAATGAAAAGCCTGATGAGATTACCTTTCTTGCTGTTCTGTCAGCTTGTGCGCATGGAGGTTTAGTAGATGAAGGCATGGAAATTTTCTCCAAGATGCAGAAGAAATATGTCAAAAATATGCCCATCAAACCCAATGACATGGTTTGGGGAGCAACGCTTGGAGCTTGCCGGATTTATATGGATATGGAAATGACAGAACAGGTAGCAAAAGATATTAGCACGCTAAATTCAAGCATTCGGTCAGCCGATAATTCACATTATGTGCTTCTGTCAAATATTTATGCTGCTTCTGATAGATGGGAAAAAGCTGAGAGGACGAGAATGGACTTGGTAAACGAAGGCTTTGAAAAGACACCTGCCCGCAGTGCATTTATGCCCTGTGGCACATAG

Protein Analysis

359

Amino Acids

40.4

Weight (kDa)

5.03

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 37 - 70 2.2e-08 PPR repeat family
PPR_1 PF12854 38 - 65 9.9e-07 PPR repeat
PPR PF01535 40 - 70 8.1e-09 PPR repeat
PPR PF01535 72 - 99 5.2e-06 PPR repeat
PPR_2 PF13041 100 - 147 9.3e-11 PPR repeat family
PPR PF01535 103 - 132 2.3e-08 PPR repeat
PPR_2 PF13041 199 - 247 8.7e-10 PPR repeat family
PPR PF01535 202 - 228 4.1e-06 PPR repeat
E_motif PF20431 288 - 349 1.4e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015465)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G21470
fragaria_vesca FvH4_2g14800 FvH4_7g25011
malus_domestica MD05G1012400.v1.1
prunus_persica Prupe.8G017700_v2.0.a1
pyrus_communis pycom05g00720
rosa_chinensis RchiOBHm_Chr6g0277371
rosa_laevigata RLG00000013306 RLG00000029733
rosa_multiflora Rmu_co8197674.1_g000001 Rmu_sc0000030.1_g000011
rosa_roxburghii Rroxscaffold_7G00191390
rosa_rugosa Rorug01G0093200 Rorug06G0107700
rosa_samantha Rh6AG218600 Rh6DG215300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1051
Acc16I TGCGCA 2 cut(s) 336, 738
Acc36I ACCTGC 1 cut(s) 1051
AccI GTMKAC 1 cut(s) 528
AccIII TCCGGA 1 cut(s) 294
AciI CCGC 2 cut(s) 10, 1048
AcsI RAATTY 3 cut(s) 313, 768, 913
AcuI CTGAAG 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 301
AflIII ACRYGT 2 cut(s) 480, 531
AgsI TTSAA 8 cut(s) 109, 244, 364, 624, 662, 687, 918, 1034
AluBI AGCT 5 cut(s) 359, 386, 731, 851, 995
AluI AGCT 5 cut(s) 359, 386, 731, 851, 995
Aor13HI TCCGGA 1 cut(s) 294
ApeKI GCWGC 1 cut(s) 971
ApoI RAATTY 3 cut(s) 313, 768, 913
AspLEI GCGC 3 cut(s) 204, 337, 739
AsuHPI GGTGA 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 186
BbvI GCAGC 1 cut(s) 958
BccI CCATC 6 cut(s) 127, 134, 157, 163, 818, 978
BclI TGATCA 2 cut(s) 88, 555
BfaI CTAG 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 1051
BisI GCNGC 1 cut(s) 972
BlsI GCNGC 1 cut(s) 973
BmiI GGNNCC 1 cut(s) 96
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 1 cut(s) 771
BmuI ACTGGG 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 123
BsaWI WCCGGW 2 cut(s) 100, 294
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 735, 765
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 61, 290
Bse3DI GCAATG 3 cut(s) 5, 160, 198
BseAI TCCGGA 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 3 cut(s) 5, 160, 198
BseMII CTCAG 3 cut(s) 455, 579, 987
BseNI ACTGG 2 cut(s) 61, 290
BseRI GAGGAG 2 cut(s) 275, 593
BseXI GCAGC 1 cut(s) 958
BseYI CCCAGC 1 cut(s) 198
BsiSI CCGG 3 cut(s) 101, 295, 856
BslI CCNNNNNNNGG 1 cut(s) 301
BsmI GAATGC 2 cut(s) 616, 921
Bsp119I TTCGAA 1 cut(s) 186
Bsp13I TCCGGA 1 cut(s) 294
Bsp143I GATC 2 cut(s) 88, 555
BspACI CCGC 2 cut(s) 10, 1048
BspCNI CTCAG 3 cut(s) 454, 580, 988
BspEI TCCGGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 96
BspMI ACCTGC 1 cut(s) 1051
BspT104I TTCGAA 1 cut(s) 186
BsrDI GCAATG 3 cut(s) 5, 160, 198
BsrI ACTGG 2 cut(s) 61, 290
BssMI GATC 2 cut(s) 88, 555
Bst4CI ACNGT 5 cut(s) 67, 130, 415, 599, 645
BstBAI YACGTR 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 186
BstC8I GCNNGC 4 cut(s) 384, 853, 908, 1048
BstDEI CTNAG 4 cut(s) 267, 441, 588, 996
BstHHI GCGC 3 cut(s) 204, 337, 739
BstKTI GATC 2 cut(s) 91, 558
BstMBI GATC 2 cut(s) 88, 555
BstMWI GCNNNNNNNGC 2 cut(s) 365, 1052
BstNSI RCATGY 3 cut(s) 382, 484, 535
BstV1I GCAGC 1 cut(s) 958
BstXI CCANNNNNNTGG 1 cut(s) 826
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 604, 650, 1057
BveI ACCTGC 1 cut(s) 1051
Cac8I GCNNGC 4 cut(s) 384, 853, 908, 1048
CfoI GCGC 3 cut(s) 204, 337, 739
CspCI CAANNNNNGTGG 2 cut(s) 87, 122
CviAII CATG 8 cut(s) 379, 481, 532, 628, 638, 740, 763, 826
DdeI CTNAG 4 cut(s) 267, 441, 588, 996
DpnI GATC 2 cut(s) 90, 557
DpnII GATC 2 cut(s) 88, 555
Eco57I CTGAAG 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 313
FaeI CATG 8 cut(s) 382, 484, 535, 631, 641, 743, 766, 829
FatI CATG 8 cut(s) 378, 480, 531, 627, 637, 739, 762, 825
FauI CCCGC 1 cut(s) 1055
FbaI TGATCA 2 cut(s) 88, 555
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 1 cut(s) 972
Fsp4HI GCNGC 1 cut(s) 972
FspAI RTGCGCAY 1 cut(s) 738
FspBI CTAG 1 cut(s) 75
FspI TGCGCA 2 cut(s) 336, 738
GlaI GCGC 3 cut(s) 203, 336, 738
GluI GCNGC 1 cut(s) 972
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 78
HapII CCGG 3 cut(s) 101, 295, 856
HhaI GCGC 3 cut(s) 204, 337, 739
Hin1II CATG 8 cut(s) 382, 484, 535, 631, 641, 743, 766, 829
Hin6I GCGC 3 cut(s) 202, 335, 737
HinP1I GCGC 3 cut(s) 202, 335, 737
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 2 cut(s) 285, 683
HpaII CCGG 3 cut(s) 101, 295, 856
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 5 cut(s) 126, 411, 514, 529, 1021
Hpy188I TCNGA 2 cut(s) 507, 979
Hpy188III TCNNGA 4 cut(s) 244, 295, 327, 659
Hpy8I GTNNAC 5 cut(s) 126, 411, 514, 529, 1021
HpyAV CCTTC 3 cut(s) 383, 752, 1019
HpyCH4III ACNGT 5 cut(s) 67, 130, 415, 599, 645
HpyCH4IV ACGT 2 cut(s) 14, 122
HpyCH4V TGCA 6 cut(s) 382, 539, 563, 648, 784, 1055
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 1052
HpyF3I CTNAG 4 cut(s) 267, 441, 588, 996
HpySE526I ACGT 2 cut(s) 14, 122
Hsp92II CATG 8 cut(s) 382, 484, 535, 631, 641, 743, 766, 829
HspAI GCGC 3 cut(s) 202, 335, 737
Kpn2I TCCGGA 1 cut(s) 294
Ksp22I TGATCA 2 cut(s) 88, 555
Kzo9I GATC 2 cut(s) 88, 555
LmnI GCTCC 2 cut(s) 836, 848
Lsp1109I GCAGC 1 cut(s) 958
LweI GCATC 1 cut(s) 771
MaeI CTAG 1 cut(s) 75
MaeII ACGT 2 cut(s) 14, 122
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 90, 557
MboI GATC 2 cut(s) 88, 555
MboII GAAGA 2 cut(s) 121, 799
MluCI AATT 6 cut(s) 307, 313, 662, 768, 913, 937
MmeI TCCRAC 1 cut(s) 587
MroI TCCGGA 1 cut(s) 294
MseI TTAA 2 cut(s) 485, 501
MspI CCGG 3 cut(s) 101, 295, 856
Mva1269I GAATGC 2 cut(s) 616, 921
MwoI GCNNNNNNNGC 2 cut(s) 365, 1052
NdeII GATC 2 cut(s) 88, 555
NlaIII CATG 8 cut(s) 382, 484, 535, 631, 641, 743, 766, 829
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 1 cut(s) 19
NsbI TGCGCA 2 cut(s) 336, 738
NspI RCATGY 3 cut(s) 382, 484, 535
NspV TTCGAA 1 cut(s) 186
PaqCI CACCTGC 1 cut(s) 1051
PciI ACATGT 2 cut(s) 480, 531
PctI GAATGC 2 cut(s) 616, 921
PfeI GAWTC 2 cut(s) 285, 683
PkrI GCNGC 1 cut(s) 973
Ppu21I YACGTR 1 cut(s) 123
PscI ACATGT 2 cut(s) 480, 531
PspFI CCCAGC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 96
SaqAI TTAA 2 cut(s) 485, 501
SatI GCNGC 1 cut(s) 972
Sau3AI GATC 2 cut(s) 88, 555
SfaNI GCATC 1 cut(s) 771
SfuI TTCGAA 1 cut(s) 186
Sse9I AATT 6 cut(s) 307, 313, 662, 768, 913, 937
SsiI CCGC 2 cut(s) 10, 1048
SspI AATATT 1 cut(s) 964
SspMI CTAG 1 cut(s) 75
TaaI ACNGT 5 cut(s) 67, 130, 415, 599, 645
TaiI ACGT 2 cut(s) 17, 125
TaqI TCGA 2 cut(s) 186, 317
TaqII GACCGA 1 cut(s) 915
TasI AATT 6 cut(s) 307, 313, 662, 768, 913, 937
TfiI GAWTC 2 cut(s) 285, 683
Tru1I TTAA 2 cut(s) 485, 501
Tru9I TTAA 2 cut(s) 485, 501
TscAI CASTG 3 cut(s) 604, 650, 1057
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 971
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 3 cut(s) 626, 705, 771
TspRI CASTG 3 cut(s) 604, 650, 1057
XapI RAATTY 3 cut(s) 313, 768, 913
XceI RCATGY 3 cut(s) 382, 484, 535
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.