FvH4_7g25710

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006571
Physical Location & Seq
Reverse (-)
19623463 .. 19626969
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25710.t1

Sequence Viewer

Length: 708 bp
ATGGAGGCTTCACACGAATTGTGTTCCATAGAGGAAGATGGGGATGGTGAGAAGGCCATGACTACCACTTCTTATCCCGCCGGCGCCTCCGACTTCCGGTTGTTTGACCGGCAAGGCTCAGTTCACGATTGCTTTGGTGGGGGACAAGCTGCTGATGTTCTGTTGTGGAGACGGCGGCGTGTTTCTATCGGTGCTATTGTTGCCGCCACAGTCTCTTGGCTTCTGTTAGAGCGGTCTGGATTATCATGCTTAACAGTTTGCTCAGATATCTTGCTGATTTTGGTTGTATTGCAATTCCTTCGAGCCAACTATGCTGTTTTTCGAAATAGACAATTGCAAGCAATACCAGAGCTAGTGGTATCAGAGGAAATGGTTAACAATGCCGCCGCATCATTTCGGGTTAAAATCAACAACGTGCTGCTTATGGCTCATGACATCACTATTGGGAAAGATTTTAAATTGTTTTTCAAGGTGGTGGTTTGTTTGTGGCTCATGTCTGTCATTGGCAGCTTTTTCTCTTTCTTCACCCTTGCTTATATTGGTTCAGTCATGTCCATAACAATTCCTGCCTTGTTCAGCAAATATGAAGGGACTGTGGATAAGTATTGTGGAAAGATCCACCGCCAATTTTCAAGGCATTATAAAGTCGTGGATGAGAGTGTTTTTAACAGACTCCCCAGAAATACATCCAAAGACAAAGACCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

236

Amino Acids

26.34

Weight (kDa)

8.2

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 51 - 205 1.3e-46 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AccB1I GGYRCC 1 cut(s) 83
AccBSI CCGCTC 1 cut(s) 232
AciI CCGC 7 cut(s) 78, 175, 204, 232, 384, 387, 622
AclWI GGATC 1 cut(s) 610
AcyI GRCGYC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 2 cut(s) 469, 633
AluBI AGCT 3 cut(s) 149, 352, 510
AluI AGCT 3 cut(s) 149, 352, 510
Alw26I GTCTC 2 cut(s) 163, 217
AlwI GGATC 1 cut(s) 610
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 3 cut(s) 149, 418, 507
AspLEI GCGC 1 cut(s) 86
AsuHPI GGTGA 2 cut(s) 59, 517
AsuII TTCGAA 1 cut(s) 322
BanI GGYRCC 1 cut(s) 83
BbvI GCAGC 3 cut(s) 136, 405, 519
BccI CCATC 2 cut(s) 32, 38
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BcoDI GTCTC 2 cut(s) 163, 217
BfaI CTAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 87
BisI GCNGC 7 cut(s) 150, 176, 204, 384, 387, 419, 508
BlsI GCNGC 7 cut(s) 151, 177, 205, 385, 388, 420, 509
BmiI GGNNCC 1 cut(s) 85
BmsI GCATC 1 cut(s) 398
Bpu14I TTCGAA 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 84
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse118I RCCGGY 2 cut(s) 80, 108
BseGI GGATG 3 cut(s) 49, 658, 686
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 132, 276
BseXI GCAGC 3 cut(s) 136, 405, 519
BshFI GGCC 1 cut(s) 56
BshNI GGYRCC 1 cut(s) 83
BsiSI CCGG 3 cut(s) 81, 97, 109
BslFI GGGAC 2 cut(s) 156, 604
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 2 cut(s) 163, 217
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 156, 604
BsnI GGCC 1 cut(s) 56
Bsp119I TTCGAA 1 cut(s) 322
Bsp143I GATC 1 cut(s) 615
BspACI CCGC 7 cut(s) 78, 175, 204, 232, 384, 387, 622
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 2 cut(s) 131, 275
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 610
BspT104I TTCGAA 1 cut(s) 322
BspT107I GGYRCC 1 cut(s) 83
BsrBI CCGCTC 1 cut(s) 232
BsrFI RCCGGY 2 cut(s) 80, 108
BssAI RCCGGY 2 cut(s) 80, 108
BssMI GATC 1 cut(s) 615
BssNI GRCGYC 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 211, 256, 595
BstACI GRCGYC 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 82, 339
BstDEI CTNAG 2 cut(s) 118, 262
BstF5I GGATG 3 cut(s) 49, 658, 686
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 1 cut(s) 618
BstMAI GTCTC 2 cut(s) 163, 217
BstMBI GATC 1 cut(s) 615
BstMWI GCNNNNNNNGC 2 cut(s) 200, 311
BstV1I GCAGC 3 cut(s) 136, 405, 519
BstX2I RGATCY 1 cut(s) 615
BstYI RGATCY 1 cut(s) 615
BsuRI GGCC 1 cut(s) 56
BtsCI GGATG 3 cut(s) 49, 658, 686
Cac8I GCNNGC 2 cut(s) 82, 339
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 2 cut(s) 80, 108
CviAII CATG 5 cut(s) 58, 246, 431, 493, 550
DdeI CTNAG 2 cut(s) 118, 262
DinI GGCGCC 1 cut(s) 85
DpnI GATC 1 cut(s) 617
DpnII GATC 1 cut(s) 615
DraI TTTAAA 1 cut(s) 457
Eco32I GATATC 1 cut(s) 268
EcoRV GATATC 1 cut(s) 268
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 5 cut(s) 61, 249, 434, 496, 553
FaqI GGGAC 2 cut(s) 156, 604
FatI CATG 5 cut(s) 57, 245, 430, 492, 549
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 7 cut(s) 150, 176, 204, 384, 387, 419, 508
FokI GGATG 3 cut(s) 56, 665, 673
Fsp4HI GCNGC 7 cut(s) 150, 176, 204, 384, 387, 419, 508
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 85
GluI GCNGC 7 cut(s) 150, 176, 204, 384, 387, 419, 508
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 56
HapII CCGG 3 cut(s) 81, 97, 109
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 5 cut(s) 61, 249, 434, 496, 553
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 1 cut(s) 672
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 3 cut(s) 81, 97, 109
HphI GGTGA 2 cut(s) 59, 517
Hpy166II GTNNAC 2 cut(s) 124, 376
Hpy188I TCNGA 3 cut(s) 91, 265, 364
Hpy188III TCNNGA 3 cut(s) 125, 237, 431
Hpy8I GTNNAC 2 cut(s) 124, 376
HpyAV CCTTC 3 cut(s) 46, 308, 581
HpyCH4III ACNGT 3 cut(s) 211, 256, 595
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 2 cut(s) 292, 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 311
HpyF3I CTNAG 2 cut(s) 118, 262
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 5 cut(s) 61, 249, 434, 496, 553
HspAI GCGC 1 cut(s) 84
KasI GGCGCC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 80
KroNI GCCGGC 1 cut(s) 82
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 1 cut(s) 615
LpnPI CCDG 7 cut(s) 94, 110, 122, 222, 360, 579, 691
Lsp1109I GCAGC 3 cut(s) 136, 405, 519
LweI GCATC 1 cut(s) 398
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 414
MalI GATC 1 cut(s) 617
MbiI CCGCTC 1 cut(s) 232
MboI GATC 1 cut(s) 615
MboII GAAGA 2 cut(s) 47, 514
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 1 cut(s) 615
MluCI AATT 6 cut(s) 17, 293, 332, 458, 561, 626
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 666
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 3 cut(s) 25, 97, 358
MreI CGCCGGCG 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 80
MseI TTAA 6 cut(s) 251, 375, 402, 456, 666, 706
MspI CCGG 3 cut(s) 81, 97, 109
MunI CAATTG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 200, 311
NaeI GCCGGC 1 cut(s) 82
NarI GGCGCC 1 cut(s) 84
NdeII GATC 1 cut(s) 615
NgoMIV GCCGGC 1 cut(s) 80
NlaIII CATG 5 cut(s) 61, 249, 434, 496, 553
NlaIV GGNNCC 1 cut(s) 85
NspV TTCGAA 1 cut(s) 322
PagI TCATGA 1 cut(s) 430
PdiI GCCGGC 1 cut(s) 82
PkrI GCNGC 7 cut(s) 151, 177, 205, 385, 388, 420, 509
PleI GAGTC 1 cut(s) 666
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 666
PsiI TTATAA 1 cut(s) 642
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 615
SaqAI TTAA 6 cut(s) 251, 375, 402, 456, 666, 706
SatI GCNGC 7 cut(s) 150, 176, 204, 384, 387, 419, 508
Sau3AI GATC 1 cut(s) 615
SchI GAGTC 1 cut(s) 666
SetI ASST 5 cut(s) 151, 354, 417, 474, 512
SfaNI GCATC 1 cut(s) 398
SfoI GGCGCC 1 cut(s) 85
SfuI TTCGAA 1 cut(s) 322
SgrAI CRCCGGYG 1 cut(s) 80
Sse9I AATT 6 cut(s) 17, 293, 332, 458, 561, 626
SsiI CCGC 7 cut(s) 78, 175, 204, 232, 384, 387, 622
SspDI GGCGCC 1 cut(s) 83
SspMI CTAG 1 cut(s) 353
TaaI ACNGT 3 cut(s) 211, 256, 595
TaiI ACGT 1 cut(s) 417
TaqI TCGA 2 cut(s) 301, 322
TasI AATT 6 cut(s) 17, 293, 332, 458, 561, 626
TauI GCSGC 4 cut(s) 178, 206, 386, 389
Tru1I TTAA 6 cut(s) 251, 375, 402, 456, 666, 706
Tru9I TTAA 6 cut(s) 251, 375, 402, 456, 666, 706
TseI GCWGC 3 cut(s) 149, 418, 507
TspDTI ATGAA 1 cut(s) 600
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.