Rorug01G0367400

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006571
Physical Location & Seq
Forward (+)
48296125 .. 48297978
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0367400.1

Sequence Viewer

Length: 1602 bp
ATGGCTGGTAAAGAAGGTCATGCAATCGGAATTGATCTCGGCACAAAGTACTCATGTGTGGGAGTTTGGAAGCATGATAACAATAATGTTGATATCATAGTCAACGATCAGGGCAACAGGACCACGCCGTCATGTGCTGCCTTCACTGATACTGAGCTTCTGGTAGGAGAAGCAGCGTTTAATCAGGTCGTGAGAAACCCCGACAACTCCATCTTTGATGCAAAGCGGTTAATTGGCAGGAAATTCAGTGATACGTCTGTTCAAAGTGACAAGAAGCTCTGGCCATTCAAGGTGGTTGAAGGTCCTGATGATAAACCTATTATTTTGGTTAAGCACGAAGGCAAAGAGAAACAGTATGCTGCTGAAGAGATCTCATCCATGGTTCTTGCAAAAATGCGGGAGATTGCTGAAGCCTATCTTGGTTCAACTGTGAAGAATGCAGTGATCACGGTCCCTGCTTACTTTAATAATTCACAGCGTGAGGCTACAAAAAAAGCTGGTATTACTGCAGGCCTAAATGTGATGCGTATTATCAATGAACCAACTGCAGCAGCCATTGCTTATGGCCTTGACAAGAAGGCTGGTTGGTATAGCAAGAGAAACGTACTCATATTTGATTTGGGTGGGGGTACTTTGGATGTGTCACTACTTACCATAGGTGATGGTGTCTTTGAAGTGAAAGCCACTGCTGGAGATACTCATCTTGGAGGTGAGGACTTCGATACCAAAATGGTGAATTACTGTGTTCAGGAATTTAAGAGGAAGCACAATTTGGATGTCAGTGGAAACATCAGAACTCTTAGGAGGTTAAGAAATGCATGTGAAATGGCAAAGAGGAGACTTTCATTTACAACGACAACTGACATTGAAATTGAATGTTTGCATCAAGGTATTGATTTCTGTGTGACTTTTACTCGTGCCAAATTTGAACAGCTCAACCTTGATTTCTTTAATAAGTGTATGGAGCCTGTGGAGAAGTGTTTGGAGGATGCCAAAATGGACGTAGGTAGTGTCCATGATGTCGTTCTTGCTGGTGGCTCTTCTAGAATCCCCAAGGTGCAACAACTGCTACAGAATGTGTTCAAGGGGAAGGAGTTGTGCAAGAGCATAAATCCTGATGAGGCAATAGCTTATGGTGCAGCTGTTCAAGCTGCAGTCTTGACAGGTGGGAACTTCCTTGGGAAACTTCAAGACTTCAGTCTCTTGGATGTCACCCCTCTCTCACTTGGAGTGGAAAGTACTGATACTACTGACCCTCACAACTTCAAAACATACATGAATGTAGTGATTCCAAGAAACTCTCGCGTTCCCATCAAGAAGAAGAAGATCTTAGTAACTGTTTATGACAACCAACGTTCAGTTGGCTTCCCTGTTTATGAGGGTGAGAGTAGAATAGCCAAACAAAATAACTTTTTAGGTAATTTTTCACTTGATGGCATTCCTCCAGTTCCTAAAGGTGAAGCCAAATTTGATGTTTGCTTTGATATTGATGCAAATGGTATCCTGAGTGTCTCTGCTGAGGACATGTCCACAGGCCAGAAGAAAGGGATTACAATCAACAGTGATAGAAGAACTTTTGAAGGTATTGAGAATGCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

533

Amino Acids

58.6

Weight (kDa)

6.73

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 8 - 524 4.7e-202 Hsp70 protein
MreB_Mbl PF06723 142 - 381 1e-16 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AccII CGCG 1 cut(s) 1305
AciI CCGC 2 cut(s) 226, 397
AclI AACGTT 1 cut(s) 1354
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 4 cut(s) 242, 752, 923, 1466
AcuI CTGAAG 3 cut(s) 384, 429, 1180
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 4 cut(s) 50, 606, 631, 1240
AflIII ACRYGT 1 cut(s) 1524
AjuI GAANNNNNNNTTGG 4 cut(s) 402, 434, 755, 787
AluBI AGCT 7 cut(s) 157, 277, 497, 934, 1130, 1142, 1151
AluI AGCT 7 cut(s) 157, 277, 497, 934, 1130, 1142, 1151
Alw26I GTCTC 3 cut(s) 832, 1205, 1516
AoxI GGCC 4 cut(s) 281, 511, 565, 1534
ApeKI GCWGC 7 cut(s) 137, 173, 359, 548, 551, 1139, 1151
ApoI RAATTY 4 cut(s) 242, 752, 923, 1466
Asp700I GAANNNNTTC 1 cut(s) 1079
AspS9I GGNCC 3 cut(s) 120, 302, 451
AsuHPI GGTGA 6 cut(s) 671, 722, 745, 1204, 1394, 1469
AvaII GGWCC 3 cut(s) 120, 302, 451
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BalI TGGCCA 1 cut(s) 283
BauI CACGAG 1 cut(s) 915
BbvCI CCTCAGC 1 cut(s) 1518
BbvI GCAGC 7 cut(s) 124, 185, 346, 560, 563, 1138, 1151
BccI CCATC 4 cut(s) 218, 656, 1319, 1427
BceAI ACGGC 1 cut(s) 112
BciVI GTATCC 1 cut(s) 1511
BclI TGATCA 1 cut(s) 444
BcoDI GTCTC 3 cut(s) 832, 1205, 1516
BfaI CTAG 1 cut(s) 1044
BfmI CTRYAG 4 cut(s) 507, 546, 1070, 1152
BfuI GTATCC 1 cut(s) 1511
BglII AGATCT 2 cut(s) 369, 1326
BisI GCNGC 7 cut(s) 138, 174, 360, 549, 552, 1140, 1152
BlsI GCNGC 7 cut(s) 139, 175, 361, 550, 553, 1141, 1153
BmcAI AGTACT 2 cut(s) 50, 1240
Bme18I GGWCC 3 cut(s) 120, 302, 451
BmgT120I GGNCC 3 cut(s) 120, 302, 451
BmiI GGNNCC 2 cut(s) 453, 966
BmsI GCATC 5 cut(s) 208, 513, 892, 979, 1480
BoxI GACNNNNGTC 1 cut(s) 1197
BpmI CTGGAG 2 cut(s) 711, 1428
Bpu10I CCTNAGC 1 cut(s) 1518
BsaBI GATNNNNATC 2 cut(s) 699, 1553
BsaJI CCNNGG 3 cut(s) 378, 1053, 1177
Bse1I ACTGG 1 cut(s) 1445
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 2 cut(s) 699, 1553
BseDI CCNNGG 3 cut(s) 378, 1053, 1177
BseGI GGATG 5 cut(s) 374, 643, 781, 994, 1213
BseJI GATNNNNATC 2 cut(s) 699, 1553
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 3 cut(s) 144, 1496, 1509
BseNI ACTGG 1 cut(s) 1445
BseRI GAGGAG 1 cut(s) 850
BseXI GCAGC 7 cut(s) 124, 185, 346, 560, 563, 1138, 1151
BsgI GTGCAG 1 cut(s) 1158
Bsh1236I CGCG 1 cut(s) 1305
BshFI GGCC 4 cut(s) 283, 513, 567, 1536
BslFI GGGAC 1 cut(s) 437
BsmAI GTCTC 3 cut(s) 832, 1205, 1516
BsmFI GGGAC 1 cut(s) 437
BsmI GAATGC 3 cut(s) 442, 1437, 1597
BsnI GGCC 4 cut(s) 283, 513, 567, 1536
Bsp143I GATC 5 cut(s) 34, 106, 369, 444, 1326
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 2 cut(s) 226, 397
BspANI GGCC 4 cut(s) 283, 513, 567, 1536
BspCNI CTCAG 3 cut(s) 145, 1497, 1510
BspFNI CGCG 1 cut(s) 1305
BspLI GGNNCC 2 cut(s) 453, 966
BspMAI CTGCAG 3 cut(s) 511, 550, 1156
BspQI GCTCTTC 1 cut(s) 1045
BsrDI GCAATG 1 cut(s) 555
BsrI ACTGG 1 cut(s) 1445
BssECI CCNNGG 3 cut(s) 378, 1053, 1177
BssMI GATC 5 cut(s) 34, 106, 369, 444, 1326
BssSI CACGAG 1 cut(s) 915
BssT1I CCWWGG 3 cut(s) 378, 1053, 1177
Bst2BI CACGAG 1 cut(s) 915
Bst4CI ACNGT 6 cut(s) 354, 430, 451, 743, 1339, 1562
Bst6I CTCTTC 2 cut(s) 360, 1045
BstAPI GCANNNNNTGC 2 cut(s) 557, 1066
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 5 cut(s) 153, 800, 1330, 1505, 1518
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 5 cut(s) 374, 643, 781, 994, 1213
BstFNI CGCG 1 cut(s) 1305
BstKTI GATC 5 cut(s) 37, 109, 372, 447, 1329
BstMAI GTCTC 3 cut(s) 832, 1205, 1516
BstMBI GATC 5 cut(s) 34, 106, 369, 444, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 557, 1066, 1136, 1148
BstNSI RCATGY 2 cut(s) 822, 1528
BstPAI GACNNNNGTC 1 cut(s) 1197
BstSFI CTRYAG 4 cut(s) 507, 546, 1070, 1152
BstUI CGCG 1 cut(s) 1305
BstV1I GCAGC 7 cut(s) 124, 185, 346, 560, 563, 1138, 1151
BstX2I RGATCY 2 cut(s) 369, 1326
BstYI RGATCY 2 cut(s) 369, 1326
BsuI GTATCC 1 cut(s) 1511
BsuRI GGCC 4 cut(s) 283, 513, 567, 1536
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 5 cut(s) 374, 643, 781, 994, 1213
BtsI GCAGTG 2 cut(s) 447, 684
BtsIMutI CAGTG 6 cut(s) 144, 253, 447, 684, 787, 1567
Cac8I GCNNGC 1 cut(s) 511
Cfr13I GGNCC 3 cut(s) 120, 302, 451
Csp6I GTAC 4 cut(s) 49, 605, 630, 1239
CviAII CATG 9 cut(s) 20, 54, 74, 132, 379, 819, 1016, 1276, 1525
CviQI GTAC 4 cut(s) 49, 605, 630, 1239
DdeI CTNAG 5 cut(s) 153, 800, 1330, 1505, 1518
DpnI GATC 5 cut(s) 36, 108, 371, 446, 1328
DpnII GATC 5 cut(s) 34, 106, 369, 444, 1326
DraIII CACNNNGTG 1 cut(s) 479
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 281
Eam1104I CTCTTC 2 cut(s) 360, 1045
EarI CTCTTC 2 cut(s) 360, 1045
Eco130I CCWWGG 3 cut(s) 378, 1053, 1177
Eco147I AGGCCT 1 cut(s) 513
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 3 cut(s) 120, 302, 451
Eco57I CTGAAG 3 cut(s) 384, 429, 1180
EcoO109I RGGNCCY 1 cut(s) 302
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 3 cut(s) 378, 1053, 1177
EcoT22I ATGCAT 1 cut(s) 820
ErhI CCWWGG 3 cut(s) 378, 1053, 1177
FaeI CATG 9 cut(s) 23, 57, 77, 135, 382, 822, 1019, 1279, 1528
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 1313, 1345
FaqI GGGAC 1 cut(s) 437
FatI CATG 9 cut(s) 19, 53, 73, 131, 378, 818, 1015, 1275, 1524
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 1 cut(s) 444
Fnu4HI GCNGC 7 cut(s) 138, 174, 360, 549, 552, 1140, 1152
FokI GGATG 5 cut(s) 361, 650, 788, 1001, 1220
Fsp4HI GCNGC 7 cut(s) 138, 174, 360, 549, 552, 1140, 1152
FspBI CTAG 1 cut(s) 1044
GluI GCNGC 7 cut(s) 138, 174, 360, 549, 552, 1140, 1152
GsuI CTGGAG 2 cut(s) 711, 1428
HaeIII GGCC 4 cut(s) 283, 513, 567, 1536
Hin1II CATG 9 cut(s) 23, 57, 77, 135, 382, 822, 1019, 1279, 1528
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 2 cut(s) 1047, 1288
HphI GGTGA 6 cut(s) 671, 722, 745, 1204, 1394, 1469
Hpy166II GTNNAC 2 cut(s) 103, 1530
Hpy188I TCNGA 2 cut(s) 29, 794
Hpy188III TCNNGA 9 cut(s) 190, 305, 749, 1044, 1115, 1159, 1190, 1315, 1504
Hpy8I GTNNAC 2 cut(s) 103, 1530
HpyAV CCTTC 7 cut(s) 8, 151, 293, 332, 571, 1084, 1574
HpyCH4III ACNGT 6 cut(s) 354, 430, 451, 743, 1339, 1562
HpyCH4IV ACGT 4 cut(s) 254, 603, 1002, 1354
HpyF10VI GCNNNNNNNGC 4 cut(s) 557, 1066, 1136, 1148
HpyF3I CTNAG 5 cut(s) 153, 800, 1330, 1505, 1518
HpySE526I ACGT 4 cut(s) 254, 603, 1002, 1354
Hsp92II CATG 9 cut(s) 23, 57, 77, 135, 382, 822, 1019, 1279, 1528
Ksp22I TGATCA 1 cut(s) 444
Kzo9I GATC 5 cut(s) 34, 106, 369, 444, 1326
LguI GCTCTTC 1 cut(s) 1045
LmnI GCTCC 1 cut(s) 964
Lsp1109I GCAGC 7 cut(s) 124, 185, 346, 560, 563, 1138, 1151
LweI GCATC 5 cut(s) 208, 513, 892, 979, 1480
MaeI CTAG 1 cut(s) 1044
MaeII ACGT 4 cut(s) 254, 603, 1002, 1354
MaeIII GTNAC 5 cut(s) 266, 642, 904, 1210, 1333
MalI GATC 5 cut(s) 36, 108, 371, 446, 1328
MboI GATC 5 cut(s) 34, 106, 369, 444, 1326
MboII GAAGA 8 cut(s) 377, 445, 1032, 1330, 1333, 1336, 1552, 1581
MflI RGATCY 2 cut(s) 369, 1326
MlsI TGGCCA 1 cut(s) 283
MluNI TGGCCA 1 cut(s) 283
Mox20I TGGCCA 1 cut(s) 283
Mph1103I ATGCAT 1 cut(s) 820
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 283
MseI TTAA 7 cut(s) 180, 230, 330, 465, 756, 809, 951
Msp20I TGGCCA 1 cut(s) 283
MspA1I CMGCKG 1 cut(s) 1142
Mva1269I GAATGC 3 cut(s) 442, 1437, 1597
MvnI CGCG 1 cut(s) 1305
MwoI GCNNNNNNNGC 4 cut(s) 557, 1066, 1136, 1148
NcoI CCATGG 1 cut(s) 378
NdeII GATC 5 cut(s) 34, 106, 369, 444, 1326
NlaIII CATG 9 cut(s) 23, 57, 77, 135, 382, 822, 1019, 1279, 1528
NlaIV GGNNCC 2 cut(s) 453, 966
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 4 cut(s) 266, 642, 904, 1210
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 2 cut(s) 822, 1528
PceI AGGCCT 1 cut(s) 513
PciI ACATGT 1 cut(s) 1524
PciSI GCTCTTC 1 cut(s) 1045
PctI GAATGC 3 cut(s) 442, 1437, 1597
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 2 cut(s) 1047, 1288
PkrI GCNGC 7 cut(s) 139, 175, 361, 550, 553, 1141, 1153
PpuMI RGGWCCY 1 cut(s) 302
PscI ACATGT 1 cut(s) 1524
PshAI GACNNNNGTC 1 cut(s) 1197
Psp1406I AACGTT 1 cut(s) 1354
Psp5II RGGWCCY 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 453, 966
PspPI GGNCC 3 cut(s) 120, 302, 451
PspPPI RGGWCCY 1 cut(s) 302
PstI CTGCAG 3 cut(s) 511, 550, 1156
PsuI RGATCY 2 cut(s) 369, 1326
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 4 cut(s) 50, 606, 631, 1240
RsaNI GTAC 4 cut(s) 49, 605, 630, 1239
SapI GCTCTTC 1 cut(s) 1045
SaqAI TTAA 7 cut(s) 180, 230, 330, 465, 756, 809, 951
SatI GCNGC 7 cut(s) 138, 174, 360, 549, 552, 1140, 1152
Sau3AI GATC 5 cut(s) 34, 106, 369, 444, 1326
Sau96I GGNCC 3 cut(s) 120, 302, 451
ScaI AGTACT 2 cut(s) 50, 1240
SfaNI GCATC 5 cut(s) 208, 513, 892, 979, 1480
SfcI CTRYAG 4 cut(s) 507, 546, 1070, 1152
SinI GGWCC 3 cut(s) 120, 302, 451
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 2 cut(s) 226, 397
SspMI CTAG 1 cut(s) 1044
StuI AGGCCT 1 cut(s) 513
StyI CCWWGG 3 cut(s) 378, 1053, 1177
TaaI ACNGT 6 cut(s) 354, 430, 451, 743, 1339, 1562
TaiI ACGT 4 cut(s) 257, 606, 1005, 1357
TaqI TCGA 1 cut(s) 720
TatI WGTACW 2 cut(s) 48, 1238
TfiI GAWTC 2 cut(s) 1047, 1288
Tru1I TTAA 7 cut(s) 180, 230, 330, 465, 756, 809, 951
Tru9I TTAA 7 cut(s) 180, 230, 330, 465, 756, 809, 951
TscAI CASTG 6 cut(s) 151, 253, 447, 691, 787, 1567
TseFI GTSAC 4 cut(s) 266, 642, 904, 1210
TseI GCWGC 7 cut(s) 137, 173, 359, 548, 551, 1139, 1151
Tsp45I GTSAC 4 cut(s) 266, 642, 904, 1210
TspDTI ATGAA 3 cut(s) 552, 834, 1292
TspRI CASTG 6 cut(s) 151, 253, 447, 691, 787, 1567
VpaK11BI GGWCC 3 cut(s) 120, 302, 451
XapI RAATTY 4 cut(s) 242, 752, 923, 1466
XbaI TCTAGA 1 cut(s) 1043
XceI RCATGY 2 cut(s) 822, 1528
XcmI CCANNNNNNNNNTGG 1 cut(s) 1358
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 1 cut(s) 1044
ZrmI AGTACT 2 cut(s) 50, 1240
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.