pycom07g20970

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006571
Physical Location & Seq
Reverse (-)
22881389 .. 22883679
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20970.1

Sequence Viewer

Length: 714 bp
ATGGAGAATTCACAAGAATTATGCAGCACAGAGGGAGATGGGGATGGACGGAAGGCGGTGCCTTCCACTTCTTACACCGCCGCATCCTGCGGCGCTGCGTATCGGTTGTTTGGCCGTCAAGATTCGGTGCACAATTGCTTGGGCGGCGGTCGAGCTGCTGATATTTTGTTATGGAAGCGGGGGCGTGTTTCCTTTGTCACTATCATTGTTGCTACAGTTGCCTGGCTTGTGTTCGAGCGGTCTGGACTATCATTCTTATCAATTTGTGCAGACGTCTTGCTGATTTTGGTTGTACTCTTATTCCTTCGTGCCAACTATGCTGATTTCAGAAATAAACAACTGCAAACATTACCGGAGCTAGTTGTATCAGAGGAAATGGTTCATAATGTTGCAGCGTCGTTCCGTGTTAAAATCAACAATGTGCTGCTTATGGCTCATGACATCACTCTTGGCAAAGACTTTAGACTATTCTTCAAGGTGGTGGTTTGTTTGTGGCTTTTGTCCGTCATTGGCAGCTTTTTCTCCTTCTTTACGCTTGCCTATATCGGTTCAATCTTGTCGATAACACTTCCAGCCTTATACAGCAAATATGAAGAGACCGTGGATAGGTGTTGCGGAAGGATCCACCGGCAGTTTTCAAAGCATTATAAAATAGTGGATGATGGTGTTTTCAACAGACTTCCCAGAAATGTACCCAAGGACAAAGATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

238

Amino Acids

26.54

Weight (kDa)

8.84

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 53 - 206 7.7e-49 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AatII GACGTC 1 cut(s) 276
AccB1I GGYRCC 1 cut(s) 58
AccBSI CCGCTC 1 cut(s) 238
AciI CCGC 9 cut(s) 56, 78, 81, 90, 144, 147, 178, 238, 615
AclWI GGATC 2 cut(s) 616, 629
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 2 cut(s) 294, 693
AfiI CCNNNNNNNGG 1 cut(s) 606
AgsI TTSAA 4 cut(s) 475, 552, 639, 673
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 3 cut(s) 155, 358, 516
AluI AGCT 3 cut(s) 155, 358, 516
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 590
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 2 cut(s) 616, 629
AoxI GGCC 1 cut(s) 112
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 6 cut(s) 24, 95, 155, 392, 424, 513
ApoI RAATTY 1 cut(s) 7
Asp700I GAANNNNTTC 1 cut(s) 378
AspLEI GCGC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 132
BamHI GGATCC 1 cut(s) 621
BanI GGYRCC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 6 cut(s) 36, 82, 142, 404, 411, 525
BccI CCATC 3 cut(s) 32, 38, 656
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 590
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 9 cut(s) 25, 81, 91, 96, 145, 156, 393, 425, 514
BlsI GCNGC 9 cut(s) 26, 82, 92, 97, 146, 157, 394, 426, 515
Bme1390I CCNGG 1 cut(s) 223
BmiI GGNNCC 2 cut(s) 60, 623
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 590
BsaJI CCNNGG 2 cut(s) 600, 696
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 627
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 600, 696
BseGI GGATG 3 cut(s) 49, 83, 664
BseLI CCNNNNNNNGG 1 cut(s) 606
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 6 cut(s) 36, 82, 142, 404, 411, 525
BsgI GTGCAG 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 2 cut(s) 353, 628
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 590
BsnI GGCC 1 cut(s) 114
Bso31I GGTCTC 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 1 cut(s) 621
BspACI CCGC 9 cut(s) 56, 78, 81, 90, 144, 147, 178, 238, 615
BspANI GGCC 1 cut(s) 114
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 60, 623
BspPI GGATC 2 cut(s) 616, 629
BspT107I GGYRCC 1 cut(s) 58
BspTNI GGTCTC 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 627
BssAI RCCGGY 1 cut(s) 627
BssECI CCNNGG 2 cut(s) 600, 696
BssMI GATC 1 cut(s) 621
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 2 cut(s) 217, 601
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 537
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 3 cut(s) 49, 83, 664
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 1 cut(s) 624
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 1 cut(s) 621
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 3 cut(s) 144, 218, 317
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 6 cut(s) 36, 82, 142, 404, 411, 525
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 3 cut(s) 49, 83, 664
Cac8I GCNNGC 1 cut(s) 537
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 627
CseI GACGC 1 cut(s) 384
Csp6I GTAC 2 cut(s) 293, 692
CviAII CATG 1 cut(s) 437
CviJI RGCY 8 cut(s) 114, 155, 226, 358, 434, 496, 516, 575
CviKI_1 RGCY 8 cut(s) 114, 155, 226, 358, 434, 496, 516, 575
CviQI GTAC 2 cut(s) 293, 692
DpnI GATC 1 cut(s) 623
DpnII GATC 1 cut(s) 621
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 696
Eco31I GGTCTC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 1 cut(s) 440
FatI CATG 1 cut(s) 436
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 9 cut(s) 25, 81, 91, 96, 145, 156, 393, 425, 514
FokI GGATG 3 cut(s) 56, 70, 671
Fsp4HI GCNGC 9 cut(s) 25, 81, 91, 96, 145, 156, 393, 425, 514
FspBI CTAG 1 cut(s) 359
GlaI GCGC 1 cut(s) 94
GluI GCNGC 9 cut(s) 25, 81, 91, 96, 145, 156, 393, 425, 514
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 114
HapII CCGG 2 cut(s) 353, 628
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 1 cut(s) 440
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 2 cut(s) 122, 707
HpaII CCGG 2 cut(s) 353, 628
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 2 cut(s) 329, 370
Hpy188III TCNNGA 3 cut(s) 119, 243, 437
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 1 cut(s) 400
HpyAV CCTTC 5 cut(s) 46, 72, 314, 535, 612
HpyCH4III ACNGT 2 cut(s) 217, 601
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 5 cut(s) 24, 130, 269, 343, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 218, 317
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 1 cut(s) 440
HspAI GCGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 621
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 8 cut(s) 100, 208, 228, 235, 366, 585, 641, 697
Lsp1109I GCAGC 6 cut(s) 36, 82, 142, 404, 411, 525
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 359
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 196
MalI GATC 1 cut(s) 623
MbiI CCGCTC 1 cut(s) 238
MboI GATC 1 cut(s) 621
MboII GAAGA 2 cut(s) 463, 605
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 4 cut(s) 7, 17, 133, 261
MnlI CCTC 2 cut(s) 25, 364
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 1 cut(s) 408
MspI CCGG 2 cut(s) 353, 628
MspR9I CCNGG 1 cut(s) 223
MunI CAATTG 1 cut(s) 133
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 3 cut(s) 144, 218, 317
NdeII GATC 1 cut(s) 621
NlaIII CATG 1 cut(s) 440
NlaIV GGNNCC 2 cut(s) 60, 623
NmuCI GTSAC 1 cut(s) 196
PagI TCATGA 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 2 cut(s) 122, 707
PkrI GCNGC 9 cut(s) 26, 82, 92, 97, 146, 157, 394, 426, 515
PsiI TTATAA 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 2 cut(s) 60, 623
PsuI RGATCY 1 cut(s) 621
RsaI GTAC 2 cut(s) 294, 693
RsaNI GTAC 2 cut(s) 293, 692
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 9 cut(s) 25, 81, 91, 96, 145, 156, 393, 425, 514
Sau3AI GATC 1 cut(s) 621
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 132
SetI ASST 6 cut(s) 157, 276, 360, 480, 518, 611
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 213
Sse9I AATT 4 cut(s) 7, 17, 133, 261
SsiI CCGC 9 cut(s) 56, 78, 81, 90, 144, 147, 178, 238, 615
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 2 cut(s) 217, 601
TaiI ACGT 1 cut(s) 276
TaqI TCGA 3 cut(s) 151, 234, 560
TasI AATT 4 cut(s) 7, 17, 133, 261
TatI WGTACW 1 cut(s) 292
TauI GCSGC 3 cut(s) 83, 93, 147
TfiI GAWTC 2 cut(s) 122, 707
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 6 cut(s) 24, 95, 155, 392, 424, 513
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 371, 606
TspGWI ACGGA 3 cut(s) 64, 392, 493
VneI GTGCAC 1 cut(s) 128
XapI RAATTY 1 cut(s) 7
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 1 cut(s) 359
ZraI GACGTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.