Rw1G033180

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006571
Physical Location & Seq
Reverse (-)
61308542 .. 61311364
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033180.1

Sequence Viewer

Length: 711 bp
ATGGAGAATTCACACGATTTGTGCTGCATAGAGGAGGAAGATGAGAATGGTAGGAAGGCCATGACTTCCACTTCTTATCCCGCCGGCGCCTCTGACTTCCGATTGTTTGGCCGGCAAGGCTCAGTTCATGATTGCTTTGGTGGTGGCCAAGCTGCTGATGTTCTGTTGTGGAGACGGCGGCGTGTTTCTATCGGTGCTATTGTTGCCGCCACAGTCTCTTGGCTTCTGTTTGAGCGATCTGGATTATCGTGCTTATCAATCTGCTCAGATATCTTGCTGATTTTGGTTGTATTGCAATTTCTTCGAGCCAACTATGCTGTTTTCCGAAATAAACAATTGCAAGCAATACCAGAGCTAGTGGTATCAGAGGAAATGGTTAACAATGCCGCTGCATCATTTCGTGTTAAAATCAACAACGTGCTGCTTATGGCTCATGACATCACTATAGGAAAAGATTTTAAATTGTTTTTCAAGGTTGTGGTTTGTCTGTGGCTCATGTCTGTCATTGGCAGCTTTTTCTCTTTCTTCACGCTTGCTTATATTGGTTCAGTCCTGTCCATAACAATTCCTGCCTTGTACAGCAAATATGAAGGGACTGTGGATAAGTATTGCGGAAAGATCCACCGGCAATTTTCAAGGCATTATAAAATCGTGGATGAAAGTGTTTTTAACAGACTTCCCAGAAATACATCCAAAGACAAAGACCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

236

Amino Acids

26.55

Weight (kDa)

8.66

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 52 - 206 4.1e-48 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AccB1I GGYRCC 1 cut(s) 86
AciI CCGC 5 cut(s) 81, 178, 207, 387, 612
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 2 cut(s) 109, 145
AcsI RAATTY 1 cut(s) 7
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 1 cut(s) 578
AgsI TTSAA 2 cut(s) 472, 636
AluBI AGCT 3 cut(s) 152, 355, 513
AluI AGCT 3 cut(s) 152, 355, 513
Alw26I GTCTC 2 cut(s) 166, 220
AlwI GGATC 1 cut(s) 613
AoxI GGCC 3 cut(s) 57, 109, 145
ApeKI GCWGC 5 cut(s) 24, 152, 389, 421, 510
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 147
BanI GGYRCC 1 cut(s) 86
BbvI GCAGC 5 cut(s) 11, 139, 376, 408, 522
BceAI ACGGC 1 cut(s) 191
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BcoDI GTCTC 2 cut(s) 166, 220
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 8 cut(s) 25, 153, 179, 207, 387, 390, 422, 511
BlsI GCNGC 8 cut(s) 26, 154, 180, 208, 388, 391, 423, 512
BmiI GGNNCC 1 cut(s) 88
BmsI GCATC 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 87
Bse118I RCCGGY 3 cut(s) 83, 111, 624
BseGI GGATG 2 cut(s) 661, 689
BseMII CTCAG 2 cut(s) 135, 279
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 5 cut(s) 11, 139, 376, 408, 522
BshFI GGCC 3 cut(s) 59, 111, 147
BshNI GGYRCC 1 cut(s) 86
BsiSI CCGG 3 cut(s) 84, 112, 625
BslFI GGGAC 1 cut(s) 607
BsmAI GTCTC 2 cut(s) 166, 220
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 3 cut(s) 59, 111, 147
Bsp1407I TGTACA 1 cut(s) 576
Bsp143I GATC 2 cut(s) 236, 618
BspACI CCGC 5 cut(s) 81, 178, 207, 387, 612
BspANI GGCC 3 cut(s) 59, 111, 147
BspCNI CTCAG 2 cut(s) 134, 278
BspHI TCATGA 2 cut(s) 127, 433
BspLI GGNNCC 1 cut(s) 88
BspPI GGATC 1 cut(s) 613
BspT107I GGYRCC 1 cut(s) 86
BsrFI RCCGGY 3 cut(s) 83, 111, 624
BsrGI TGTACA 1 cut(s) 576
BssAI RCCGGY 3 cut(s) 83, 111, 624
BssMI GATC 2 cut(s) 236, 618
BssNI GRCGYC 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 214, 598
BstACI GRCGYC 1 cut(s) 87
BstAUI TGTACA 1 cut(s) 576
BstC8I GCNNGC 4 cut(s) 85, 113, 342, 534
BstDEI CTNAG 2 cut(s) 121, 265
BstF5I GGATG 2 cut(s) 661, 689
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 2 cut(s) 239, 621
BstMAI GTCTC 2 cut(s) 166, 220
BstMBI GATC 2 cut(s) 236, 618
BstMWI GCNNNNNNNGC 3 cut(s) 117, 203, 314
BstSFI CTRYAG 1 cut(s) 444
BstV1I GCAGC 5 cut(s) 11, 139, 376, 408, 522
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
BsuRI GGCC 3 cut(s) 59, 111, 147
BtsCI GGATG 2 cut(s) 661, 689
Cac8I GCNNGC 4 cut(s) 85, 113, 342, 534
CciI TCATGA 2 cut(s) 127, 433
CfoI GCGC 1 cut(s) 89
Cfr10I RCCGGY 3 cut(s) 83, 111, 624
Csp6I GTAC 1 cut(s) 577
CviAII CATG 4 cut(s) 61, 128, 434, 496
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 2 cut(s) 121, 265
DinI GGCGCC 1 cut(s) 88
DpnI GATC 2 cut(s) 238, 620
DpnII GATC 2 cut(s) 236, 618
DraI TTTAAA 1 cut(s) 460
EaeI YGGCCR 2 cut(s) 109, 145
Eco32I GATATC 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 7
EcoRV GATATC 1 cut(s) 271
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 4 cut(s) 64, 131, 437, 499
FaqI GGGAC 1 cut(s) 607
FatI CATG 4 cut(s) 60, 127, 433, 495
FauI CCCGC 1 cut(s) 88
Fnu4HI GCNGC 8 cut(s) 25, 153, 179, 207, 387, 390, 422, 511
FokI GGATG 2 cut(s) 668, 676
Fsp4HI GCNGC 8 cut(s) 25, 153, 179, 207, 387, 390, 422, 511
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 88
GluI GCNGC 8 cut(s) 25, 153, 179, 207, 387, 390, 422, 511
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 3 cut(s) 59, 111, 147
HapII CCGG 3 cut(s) 84, 112, 625
HhaI GCGC 1 cut(s) 89
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 64, 131, 437, 499
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HpaI GTTAAC 1 cut(s) 379
HpaII CCGG 3 cut(s) 84, 112, 625
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 5 cut(s) 94, 101, 268, 326, 367
Hpy188III TCNNGA 3 cut(s) 128, 240, 434
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 2 cut(s) 49, 584
HpyCH4III ACNGT 2 cut(s) 214, 598
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 4 cut(s) 27, 295, 340, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 203, 314
HpyF3I CTNAG 2 cut(s) 121, 265
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 64, 131, 437, 499
HspAI GCGC 1 cut(s) 87
KasI GGCGCC 1 cut(s) 86
KroI GCCGGC 2 cut(s) 83, 111
KroNI GCCGGC 2 cut(s) 85, 113
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 236, 618
LpnPI CCDG 8 cut(s) 97, 125, 225, 363, 566, 582, 638, 694
Lsp1109I GCAGC 5 cut(s) 11, 139, 376, 408, 522
LweI GCATC 1 cut(s) 401
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 417
MalI GATC 2 cut(s) 238, 620
MboI GATC 2 cut(s) 236, 618
MboII GAAGA 3 cut(s) 50, 293, 517
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 618
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 6 cut(s) 7, 296, 335, 461, 564, 629
MluNI TGGCCA 1 cut(s) 147
Mly113I GGCGCC 1 cut(s) 87
MnlI CCTC 4 cut(s) 25, 28, 100, 361
Mox20I TGGCCA 1 cut(s) 147
MreI CGCCGGCG 1 cut(s) 83
MroNI GCCGGC 2 cut(s) 83, 111
MscI TGGCCA 1 cut(s) 147
MseI TTAA 5 cut(s) 378, 405, 459, 669, 709
Msp20I TGGCCA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 84, 112, 625
MunI CAATTG 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 117, 203, 314
NaeI GCCGGC 2 cut(s) 85, 113
NarI GGCGCC 1 cut(s) 87
NdeII GATC 2 cut(s) 236, 618
NgoMIV GCCGGC 2 cut(s) 83, 111
NlaIII CATG 4 cut(s) 64, 131, 437, 499
NlaIV GGNNCC 1 cut(s) 88
PagI TCATGA 2 cut(s) 127, 433
PdiI GCCGGC 2 cut(s) 85, 113
PkrI GCNGC 8 cut(s) 26, 154, 180, 208, 388, 391, 423, 512
PluTI GGCGCC 1 cut(s) 90
PsiI TTATAA 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SaqAI TTAA 5 cut(s) 378, 405, 459, 669, 709
SatI GCNGC 8 cut(s) 25, 153, 179, 207, 387, 390, 422, 511
Sau3AI GATC 2 cut(s) 236, 618
SetI ASST 5 cut(s) 154, 357, 420, 477, 515
SfaNI GCATC 1 cut(s) 401
SfcI CTRYAG 1 cut(s) 444
SfoI GGCGCC 1 cut(s) 88
SgrAI CRCCGGYG 1 cut(s) 83
Sse9I AATT 6 cut(s) 7, 296, 335, 461, 564, 629
SsiI CCGC 5 cut(s) 81, 178, 207, 387, 612
SspDI GGCGCC 1 cut(s) 86
SspMI CTAG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 214, 598
TaiI ACGT 1 cut(s) 420
TaqI TCGA 1 cut(s) 304
TasI AATT 6 cut(s) 7, 296, 335, 461, 564, 629
TatI WGTACW 1 cut(s) 576
TauI GCSGC 3 cut(s) 181, 209, 389
Tru1I TTAA 5 cut(s) 378, 405, 459, 669, 709
Tru9I TTAA 5 cut(s) 378, 405, 459, 669, 709
TseI GCWGC 5 cut(s) 24, 152, 389, 421, 510
TspDTI ATGAA 3 cut(s) 116, 603, 672
XapI RAATTY 1 cut(s) 7
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.