MD01G1164400.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006571
Physical Location & Seq
Reverse (-)
26878483 .. 26880238
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1164400.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGAGAATTCACAAGATTTATGCAGCACAGAGGGAGATGGGGATGGACGGAAGGATGTGGCTTCCACTTCTTACACCACCGCATGCGTCGGCGCTGCGTATCGGTTGTTTGGACGTCAAGAGTCGATGCACAACTGCTTGGGCGGCGGTCAAGCTGCTGATGTTTTGTTGTGGAAGCGGGGGCGTGTTTCCTTCGGCACTATCATTGTTTCTACAGTTTCTTGGCTTGTGTTTGAGCGGTCTGGATTATCATTCTTATCAGTTTGTGCGGACATCTTGCTGATTTTGGTTGTACTCTTATTCCTTCGTGCCAACTATGCTGTTTATAGAAATAAGTATGAATTTTCTACTCTCCACATGTATTATTATCAACCTTGCACAGAAACTGTTTACAATGAGGATAACTATCATATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

140

Amino Acids

15.65

Weight (kDa)

5.51

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 53 - 112 2.3e-13 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 238
AciI CCGC 6 cut(s) 81, 144, 147, 178, 238, 269
AcsI RAATTY 2 cut(s) 7, 341
AcyI GRCGYC 1 cut(s) 115
AfaI GTAC 1 cut(s) 294
AflIII ACRYGT 1 cut(s) 357
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 386
ApeKI GCWGC 3 cut(s) 24, 95, 155
ApoI RAATTY 2 cut(s) 7, 341
AspLEI GCGC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 36, 82, 142
BccI CCATC 2 cut(s) 32, 38
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 4 cut(s) 25, 96, 145, 156
BlsI GCNGC 4 cut(s) 26, 97, 146, 157
BmsI GCATC 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 115
Bse8I GATNNNNATC 1 cut(s) 405
BseGI GGATG 2 cut(s) 49, 61
BseJI GATNNNNATC 1 cut(s) 405
BseXI GCAGC 3 cut(s) 36, 82, 142
BspACI CCGC 6 cut(s) 81, 144, 147, 178, 238, 269
BsrBI CCGCTC 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 115
Bst4CI ACNGT 2 cut(s) 217, 388
BstACI GRCGYC 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 85
BstF5I GGATG 2 cut(s) 49, 61
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstMWI GCNNNNNNNGC 2 cut(s) 144, 317
BstNSI RCATGY 2 cut(s) 87, 361
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 36, 82, 142
BtsCI GGATG 2 cut(s) 49, 61
Cac8I GCNNGC 1 cut(s) 85
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 1 cut(s) 95
CseI GACGC 1 cut(s) 76
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 84, 358
CviJI RGCY 3 cut(s) 62, 155, 226
CviKI_1 RGCY 3 cut(s) 62, 155, 226
CviQI GTAC 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 7
FaeI CATG 2 cut(s) 87, 361
FaiI YATR 7 cut(s) 22, 85, 318, 327, 339, 359, 411
FatI CATG 2 cut(s) 83, 357
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 4 cut(s) 25, 96, 145, 156
FokI GGATG 2 cut(s) 56, 68
Fsp4HI GCNGC 4 cut(s) 25, 96, 145, 156
GlaI GCGC 1 cut(s) 94
GluI GCNGC 4 cut(s) 25, 96, 145, 156
HaeII RGCGCY 1 cut(s) 96
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 2 cut(s) 87, 361
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 391
Hpy188III TCNNGA 2 cut(s) 119, 243
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 46, 202, 314
HpyCH4III ACNGT 2 cut(s) 217, 388
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 3 cut(s) 24, 130, 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 317
HpySE526I ACGT 1 cut(s) 115
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 2 cut(s) 87, 361
HspAI GCGC 1 cut(s) 93
LpnPI CCDG 1 cut(s) 228
Lsp1109I GCAGC 3 cut(s) 36, 82, 142
LweI GCATC 1 cut(s) 117
MaeII ACGT 1 cut(s) 115
MbiI CCGCTC 1 cut(s) 238
MluCI AATT 2 cut(s) 7, 341
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 2 cut(s) 25, 391
MwoI GCNNNNNNNGC 2 cut(s) 144, 317
NlaIII CATG 2 cut(s) 87, 361
NspI RCATGY 2 cut(s) 87, 361
PaeI GCATGC 1 cut(s) 87
PciI ACATGT 1 cut(s) 357
PkrI GCNGC 4 cut(s) 26, 97, 146, 157
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
PscI ACATGT 1 cut(s) 357
PstNI CAGNNNCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
SatI GCNGC 4 cut(s) 25, 96, 145, 156
SchI GAGTC 1 cut(s) 131
SetI ASST 3 cut(s) 118, 157, 376
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 213
SphI GCATGC 1 cut(s) 87
Sse9I AATT 2 cut(s) 7, 341
SsiI CCGC 6 cut(s) 81, 144, 147, 178, 238, 269
TaaI ACNGT 2 cut(s) 217, 388
TaiI ACGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 125
TasI AATT 2 cut(s) 7, 341
TatI WGTACW 1 cut(s) 292
TauI GCSGC 1 cut(s) 147
TseI GCWGC 3 cut(s) 24, 95, 155
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 64
XapI RAATTY 2 cut(s) 7, 341
XceI RCATGY 2 cut(s) 87, 361
ZraI GACGTC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.