MD01G1164300.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006571
Physical Location & Seq
Reverse (-)
26877762 .. 26878481
720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1164300.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGTTAACAATGCTGCAGCATCGTTTCGTGTTAAAATCAACAATGTGCTGCTTATGGCTCATGATATCACTCTCGGCAAAGATTTTAGACTGTTTTTCAAGGTGGTGGTTTGTCTGTGGCTCTTCTCCGTCATTGGAAGCTTTTTCTCCTTCTTTACACTTGCGTATATTGGTTCAATCTTGTCAATAACACTTCCTGCCTTGTACAGCAAGCATGAAGAAACTGTGGATGGGTATTGCGGAAAGATCCACCGGCAGTTTTCAAAGCATTACAAAATCGTGGACGAGGGTGTTTTTAACAGACTCCCCAGAAATGTACCAAAGGACAAAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

113

Amino Acids

12.81

Weight (kDa)

9.39

Isoelectric Point (pI)

23.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 1 - 82 1.4e-25 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 240
AclWI GGATC 1 cut(s) 241
AfaI GTAC 2 cut(s) 206, 318
AgsI TTSAA 3 cut(s) 100, 177, 264
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
AlwI GGATC 1 cut(s) 241
ApeKI GCWGC 3 cut(s) 14, 17, 49
BbvI GCAGC 2 cut(s) 29, 36
BccI CCATC 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 3 cut(s) 15, 18, 50
BlsI GCNGC 3 cut(s) 16, 19, 51
BmsI GCATC 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 252
BseGI GGATG 1 cut(s) 235
BseXI GCAGC 2 cut(s) 29, 36
BsiSI CCGG 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 1 cut(s) 240
BspHI TCATGA 1 cut(s) 61
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 1 cut(s) 241
BspQI GCTCTTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 252
BssMI GATC 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 93, 226
Bst6I CTCTTC 1 cut(s) 128
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 212
BstF5I GGATG 1 cut(s) 235
BstKTI GATC 1 cut(s) 249
BstMBI GATC 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 29, 36
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BtsCI GGATG 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 212
CciI TCATGA 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 252
Csp6I GTAC 2 cut(s) 205, 317
CviAII CATG 2 cut(s) 62, 215
CviJI RGCY 3 cut(s) 59, 121, 141
CviKI_1 RGCY 3 cut(s) 59, 121, 141
CviQI GTAC 2 cut(s) 205, 317
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco32I GATATC 1 cut(s) 67
EcoRV GATATC 1 cut(s) 67
FaeI CATG 2 cut(s) 65, 218
FaiI YATR 4 cut(s) 56, 63, 168, 216
FatI CATG 2 cut(s) 61, 214
Fnu4HI GCNGC 3 cut(s) 15, 18, 50
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 3 cut(s) 15, 18, 50
GluI GCNGC 3 cut(s) 15, 18, 50
HapII CCGG 1 cut(s) 253
Hin1II CATG 2 cut(s) 65, 218
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 2 cut(s) 303, 332
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 7, 283
Hpy188III TCNNGA 1 cut(s) 62
Hpy8I GTNNAC 2 cut(s) 7, 283
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 93, 226
HpyCH4V TGCA 1 cut(s) 17
Hsp92II CATG 2 cut(s) 65, 218
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 246
LguI GCTCTTC 1 cut(s) 128
LpnPI CCDG 3 cut(s) 210, 266, 322
Lsp1109I GCAGC 2 cut(s) 29, 36
LweI GCATC 1 cut(s) 29
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 2 cut(s) 115, 230
MflI RGATCY 1 cut(s) 246
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 1 cut(s) 280
MseI TTAA 4 cut(s) 6, 33, 297, 337
MspI CCGG 1 cut(s) 253
NdeII GATC 1 cut(s) 246
NlaIII CATG 2 cut(s) 65, 218
NmeAIII GCCGAG 1 cut(s) 54
PagI TCATGA 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 3 cut(s) 16, 19, 51
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 2 cut(s) 206, 318
RsaNI GTAC 2 cut(s) 205, 317
SapI GCTCTTC 1 cut(s) 128
SaqAI TTAA 4 cut(s) 6, 33, 297, 337
SatI GCNGC 3 cut(s) 15, 18, 50
Sau3AI GATC 1 cut(s) 246
SchI GAGTC 1 cut(s) 297
SetI ASST 2 cut(s) 105, 143
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 1 cut(s) 15
SsiI CCGC 1 cut(s) 240
TaaI ACNGT 2 cut(s) 93, 226
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 4 cut(s) 6, 33, 297, 337
Tru9I TTAA 4 cut(s) 6, 33, 297, 337
TseI GCWGC 3 cut(s) 14, 17, 49
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.