FvH4_7g26180

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012379
Physical Location & Seq
Forward (+)
19864650 .. 19867263
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26180.t1

Sequence Viewer

Length: 849 bp
ATGTTGGAGGAGATTACCAATTTGGCAGAGGTCAGTTTGAAGAGGACTGCTACTACTCACTTTGTGCTGGTGCATGGTATCGGTGGAGGAGGTTGGTGCTGGTACAAAATCCGGTGTCTCATGGAGAAATCCGGCTACAAAGTCTCCACGGTTGATCTGAAGAGCGCCGGAATAGACCCCTCCAACGTGGATTCGGTTCTCTCTTTCGACGACTACAACAAACCACTCCTCGACTTCCTCTCTTCTCTCCCCGAATATGAACAGGTTGTGCTGGTCGGGCACAGCGCCGGAGGGTTGAGCGTGACGCAGGCGACGCTGAAGTTTCCGAAGAAGATCCGGCTGGCGGTTTATGTGGCGGCGACGATGCTCAAGCATGGCTACCTTTCTGAGGAAGATGTGAAAGATGGAGTGCCTGATTTATCCGAATTCGGTGACGTGTACGAATTAGGGTTCGGATTAGGAGCTAATCAACCACCAACAAGCGGAGTTGTCAAGAAAGATTTCCAACGCAAAATCATATATCAAATGAGTCCTCAAGAGGATTCGACTCTTGCTGCCATGCTTTTGAGACCAGGACCGCTCCTAGCTATAACGACAGCCCAGTTTAAGGAAGAAGACGATGACCATGATGACACGGGTGTGCTTGACAAGGTGCCACGTGTCTACGTGAAGACGTTGCATGATCACGTGGTGAAACCGGCGCAGCAAGATGCGATGGTCAAGCGGTGGCCGCCGGCGGAGGTCTATGTTTTGGATAGTGATCACAGTCCCTTCTTCTCCCAGCCGTTTCTGCTCTTTGGATTCCTTGTGAAGGCTGCCGAGGCTTCTCATTGCAGGGAGATTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

283

Amino Acids

31.22

Weight (kDa)

5.36

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 20 - 260 4.9e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 21 - 262 5.3e-18 Alpha/beta hydrolase family
Hydrolase_4 PF12146 21 - 124 1.4e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 580
AccI GTMKAC 1 cut(s) 663
AciI CCGC 7 cut(s) 344, 356, 483, 578, 724, 731, 737
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 1 cut(s) 425
AcuI CTGAAG 2 cut(s) 179, 338
AcvI CACGTG 2 cut(s) 659, 688
AdeI CACNNNGTG 2 cut(s) 64, 691
AfaI GTAC 2 cut(s) 104, 440
AfiI CCNNNNNNNGG 5 cut(s) 343, 388, 482, 607, 811
AflIII ACRYGT 2 cut(s) 435, 658
AgsI TTSAA 1 cut(s) 40
AjiI CACGTC 1 cut(s) 436
AjnI CCWGG 1 cut(s) 571
AleI CACNNNNGTG 1 cut(s) 638
AluBI AGCT 2 cut(s) 464, 587
AluI AGCT 2 cut(s) 464, 587
Alw26I GTCTC 3 cut(s) 122, 148, 562
AlwI GGATC 1 cut(s) 328
AoxI GGCC 1 cut(s) 728
ApeKI GCWGC 3 cut(s) 554, 703, 815
ApoI RAATTY 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 500
AspLEI GCGC 3 cut(s) 167, 287, 703
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 443, 703
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 652
BbrPI CACGTG 2 cut(s) 659, 688
BbsI GAAGAC 2 cut(s) 621, 677
BbvI GCAGC 3 cut(s) 541, 715, 802
BccI CCATC 2 cut(s) 398, 709
BceAI ACGGC 1 cut(s) 769
BciT130I CCWGG 1 cut(s) 573
BclI TGATCA 2 cut(s) 682, 760
BcoDI GTCTC 3 cut(s) 122, 148, 562
BfaI CTAG 1 cut(s) 584
BfoI RGCGCY 2 cut(s) 168, 288
BisI GCNGC 5 cut(s) 357, 555, 704, 731, 816
BlsI GCNGC 5 cut(s) 358, 556, 705, 732, 817
Bme1390I CCNGG 1 cut(s) 573
Bme18I GGWCC 1 cut(s) 575
BmgBI CACGTC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 1 cut(s) 573
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 2 cut(s) 354, 700
BmuI ACTGGG 1 cut(s) 595
BpiI GAAGAC 2 cut(s) 621, 677
BpuEI CTTGAG 2 cut(s) 353, 519
BsaAI YACGTR 3 cut(s) 659, 667, 688
BsaBI GATNNNNATC 1 cut(s) 759
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 2 cut(s) 147, 819
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 5 cut(s) 343, 388, 482, 607, 811
Bse118I RCCGGY 2 cut(s) 697, 733
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 829
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 2 cut(s) 147, 819
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 5 cut(s) 343, 388, 482, 607, 811
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 3 cut(s) 23, 102, 218
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 3 cut(s) 541, 715, 802
BseYI CCCAGC 1 cut(s) 780
BshFI GGCC 1 cut(s) 730
BshNI GGYRCC 1 cut(s) 652
BsiSI CCGG 7 cut(s) 112, 132, 168, 288, 337, 698, 734
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 5 cut(s) 343, 388, 482, 607, 811
BsmAI GTCTC 3 cut(s) 122, 148, 562
BsmFI GGGAC 1 cut(s) 753
BsnI GGCC 1 cut(s) 730
Bso31I GGTCTC 1 cut(s) 562
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 154, 333, 682, 760
BspACI CCGC 7 cut(s) 344, 356, 483, 578, 724, 731, 737
BspANI GGCC 1 cut(s) 730
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 654
BspPI GGATC 1 cut(s) 328
BspQI GCTCTTC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 652
BspTNI GGTCTC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 2 cut(s) 697, 733
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 2 cut(s) 697, 733
BssECI CCNNGG 2 cut(s) 147, 819
BssMI GATC 4 cut(s) 154, 333, 682, 760
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 2 cut(s) 151, 767
Bst6I CTCTTC 3 cut(s) 35, 155, 247
BstBAI YACGTR 3 cut(s) 659, 667, 688
BstC8I GCNNGC 3 cut(s) 309, 342, 735
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 2 cut(s) 386, 809
BstH2I RGCGCY 2 cut(s) 168, 288
BstHHI GCGC 3 cut(s) 167, 287, 703
BstKTI GATC 4 cut(s) 157, 336, 685, 763
BstMAI GTCTC 3 cut(s) 122, 148, 562
BstMBI GATC 4 cut(s) 154, 333, 682, 760
BstMWI GCNNNNNNNGC 5 cut(s) 277, 313, 730, 790, 821
BstNI CCWGG 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 571
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 541, 715, 802
BstV2I GAAGAC 2 cut(s) 621, 677
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 730
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 728
BtrI CACGTC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 309, 342, 735
CfoI GCGC 3 cut(s) 167, 287, 703
Cfr10I RCCGGY 2 cut(s) 697, 733
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 2 cut(s) 313, 322
Csp6I GTAC 2 cut(s) 103, 439
CviAII CATG 6 cut(s) 74, 121, 374, 559, 626, 680
CviQI GTAC 2 cut(s) 103, 439
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 156, 335, 684, 762
DpnII GATC 4 cut(s) 154, 333, 682, 760
DraIII CACNNNGTG 2 cut(s) 64, 691
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 3 cut(s) 35, 155, 247
EarI CTCTTC 3 cut(s) 35, 155, 247
EciI GGCGGA 1 cut(s) 752
Eco31I GGTCTC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 179, 338
Eco72I CACGTG 2 cut(s) 659, 688
EcoNI CCTNNNNNAGG 2 cut(s) 386, 809
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 571
FaeI CATG 6 cut(s) 77, 124, 377, 562, 629, 683
FaqI GGGAC 1 cut(s) 753
FatI CATG 6 cut(s) 73, 120, 373, 558, 625, 679
FbaI TGATCA 2 cut(s) 682, 760
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 5 cut(s) 357, 555, 704, 731, 816
Fsp4HI GCNGC 5 cut(s) 357, 555, 704, 731, 816
FspBI CTAG 1 cut(s) 584
GlaI GCGC 3 cut(s) 166, 286, 702
GluI GCNGC 5 cut(s) 357, 555, 704, 731, 816
GsaI CCCAGC 1 cut(s) 784
HaeII RGCGCY 2 cut(s) 168, 288
HaeIII GGCC 1 cut(s) 730
HapII CCGG 7 cut(s) 112, 132, 168, 288, 337, 698, 734
HgaI GACGC 2 cut(s) 313, 322
HhaI GCGC 3 cut(s) 167, 287, 703
Hin1II CATG 6 cut(s) 77, 124, 377, 562, 629, 683
Hin6I GCGC 3 cut(s) 165, 285, 701
HinP1I GCGC 3 cut(s) 165, 285, 701
HinfI GANTC 5 cut(s) 191, 529, 542, 547, 801
HpaII CCGG 7 cut(s) 112, 132, 168, 288, 337, 698, 734
HphI GGTGA 2 cut(s) 443, 703
Hpy166II GTNNAC 2 cut(s) 439, 664
Hpy188I TCNGA 5 cut(s) 159, 327, 388, 424, 455
Hpy188III TCNNGA 2 cut(s) 493, 536
Hpy8I GTNNAC 2 cut(s) 439, 664
Hpy99I CGWCG 3 cut(s) 212, 316, 364
HpyAV CCTTC 2 cut(s) 781, 805
HpyCH4III ACNGT 2 cut(s) 151, 767
HpyCH4IV ACGT 6 cut(s) 186, 435, 658, 666, 674, 687
HpyCH4V TGCA 3 cut(s) 73, 679, 834
HpyF10VI GCNNNNNNNGC 5 cut(s) 277, 313, 730, 790, 821
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 6 cut(s) 186, 435, 658, 666, 674, 687
Hsp92II CATG 6 cut(s) 77, 124, 377, 562, 629, 683
HspAI GCGC 3 cut(s) 165, 285, 701
KroI GCCGGC 1 cut(s) 733
KroNI GCCGGC 1 cut(s) 735
Ksp22I TGATCA 2 cut(s) 682, 760
Kzo9I GATC 4 cut(s) 154, 333, 682, 760
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 2 cut(s) 461, 585
Lsp1109I GCAGC 3 cut(s) 541, 715, 802
LweI GCATC 2 cut(s) 354, 700
MaeI CTAG 1 cut(s) 584
MaeII ACGT 6 cut(s) 186, 435, 658, 666, 674, 687
MaeIII GTNAC 2 cut(s) 301, 431
MalI GATC 4 cut(s) 156, 335, 684, 762
MbiI CCGCTC 1 cut(s) 580
MboI GATC 4 cut(s) 154, 333, 682, 760
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 3 cut(s) 19, 425, 443
MlyI GAGTC 2 cut(s) 538, 541
MmeI TCCRAC 2 cut(s) 207, 529
MreI CGCCGGCG 1 cut(s) 733
MroNI GCCGGC 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 1 cut(s) 606
MslI CAYNNNNRTG 1 cut(s) 638
MspI CCGG 7 cut(s) 112, 132, 168, 288, 337, 698, 734
MspR9I CCNGG 1 cut(s) 573
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 5 cut(s) 277, 313, 730, 790, 821
NaeI GCCGGC 1 cut(s) 735
NdeII GATC 4 cut(s) 154, 333, 682, 760
NgoMIV GCCGGC 1 cut(s) 733
NlaIII CATG 6 cut(s) 77, 124, 377, 562, 629, 683
NlaIV GGNNCC 1 cut(s) 654
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 2 cut(s) 301, 431
OliI CACNNNNGTG 1 cut(s) 638
PciSI GCTCTTC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 3 cut(s) 191, 542, 801
PkrI GCNGC 5 cut(s) 358, 556, 705, 732, 817
PleI GAGTC 2 cut(s) 537, 541
PmaCI CACGTG 2 cut(s) 659, 688
PmlI CACGTG 2 cut(s) 659, 688
PpsI GAGTC 2 cut(s) 537, 541
Ppu21I YACGTR 3 cut(s) 659, 667, 688
Psp6I CCWGG 1 cut(s) 571
PspCI CACGTG 2 cut(s) 659, 688
PspFI CCCAGC 1 cut(s) 780
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 2 cut(s) 104, 440
RsaNI GTAC 2 cut(s) 103, 439
RseI CAYNNNNRTG 1 cut(s) 638
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 5 cut(s) 357, 555, 704, 731, 816
Sau3AI GATC 4 cut(s) 154, 333, 682, 760
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 2 cut(s) 538, 541
ScrFI CCNGG 1 cut(s) 573
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 2 cut(s) 354, 700
SgrAI CRCCGGYG 1 cut(s) 733
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 2 cut(s) 368, 534
SmoI CTYRAG 2 cut(s) 368, 534
Sse9I AATT 3 cut(s) 19, 425, 443
SsiI CCGC 7 cut(s) 344, 356, 483, 578, 724, 731, 737
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 571
TaaI ACNGT 2 cut(s) 151, 767
TaiI ACGT 6 cut(s) 189, 438, 661, 669, 677, 690
TaqI TCGA 3 cut(s) 207, 231, 545
TasI AATT 3 cut(s) 19, 425, 443
TauI GCSGC 2 cut(s) 359, 733
TfiI GAWTC 3 cut(s) 191, 542, 801
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TseFI GTSAC 2 cut(s) 301, 431
TseI GCWGC 3 cut(s) 554, 703, 815
Tsp45I GTSAC 2 cut(s) 301, 431
TspDTI ATGAA 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 575
XagI CCTNNNNNAGG 2 cut(s) 386, 809
XapI RAATTY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.