pycom07g21330

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012379
Physical Location & Seq
Forward (+)
23090962 .. 23093412
2451 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21330.3

Sequence Viewer

Length: 732 bp
ATGGTGCTGGTACAAAATCCGGTGTCTCATGGAGAATTCTGGCTACAAGGTATCGTGTGTCGATCGATCAAGAGTGCCGGCATCGATCAATCTGATGCGAACTCAGTCCTTTCTTTTGATGATTACAACAAGCCACTCCTGGACTTGCTGTCTGCTCTCCCTGAGAATGAACAGGTAATTCTGGTGGGACACAGTGCTGGAGGGCTGAATGTAACTCAGGCCACTCTCAAGTTCCCAAAGAAAATTCTTTTAGCAGTATATGTTGCAGCAACAATGCTTAAACATGGGTTCTCCACTGATCAAGATTTCAAAGATGGGGTGCCAGATTTATCAGAGTTTGGTGATGTATATGAGTTAGGGTTTGGATTGGGAGCTGATAAACCTCCAACAAGCGCCATTGTCAAGAAAGAATTCCAACGCAAAATCTCATACCAATTGAGTCCTCAAGAGGATTCGACGCTAGCTGCCATGCTTTTGAGGCCAGGGCCGCTCTTGGCAATCACGTCAGCCCATTTCGAGGAGGACGGCGTCATCGACCAAGTGCCACGAGTTTACGTTAAAACGCTGCATGACCATGTCGTGAAACCAGAGCAACAAGGTTCGATGGTAAAGAGGTGGCCGCCATCGGAGCTCTATGTTTTGCATAGTGATCACAGCCCTATGTTCTCCACCCCATTTCTGCTATTTGGCTTCCTTGTAAAAGCAGCGGCTTCTTTTGGTGGACTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

244

Amino Acids

26.61

Weight (kDa)

5.72

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 319
AccBSI CCGCTC 1 cut(s) 490
AciI CCGC 3 cut(s) 488, 620, 707
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 3 cut(s) 35, 243, 410
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 517
AgsI TTSAA 1 cut(s) 310
AhdI GACNNNNNGTC 1 cut(s) 148
AjiI CACGTC 1 cut(s) 504
AjnI CCWGG 2 cut(s) 138, 481
AluBI AGCT 3 cut(s) 374, 464, 631
AluI AGCT 3 cut(s) 374, 464, 631
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 1 cut(s) 30
AoxI GGCC 4 cut(s) 219, 479, 485, 617
ApeKI GCWGC 4 cut(s) 266, 464, 565, 704
ApoI RAATTY 3 cut(s) 35, 243, 410
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 353
AsuNHI GCTAGC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 633
BauI CACGAG 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 4 cut(s) 278, 451, 552, 716
BccI CCATC 3 cut(s) 308, 598, 631
BceAI ACGGC 1 cut(s) 541
BciT130I CCWGG 2 cut(s) 140, 483
BclI TGATCA 2 cut(s) 298, 649
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 461
BfoI RGCGCY 1 cut(s) 396
BisI GCNGC 7 cut(s) 267, 465, 488, 566, 620, 705, 708
BlsI GCNGC 7 cut(s) 268, 466, 489, 567, 621, 706, 709
Bme1390I CCNGG 2 cut(s) 140, 483
BmeRI GACNNNNNGTC 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 504
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 140, 483
BmsI GCATC 2 cut(s) 85, 90
BmtI GCTAGC 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 219
BpuEI CTTGAG 2 cut(s) 212, 429
Bsa29I ATCGAT 2 cut(s) 65, 84
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 1 cut(s) 482
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 517
Bse118I RCCGGY 1 cut(s) 77
BseBI CCWGG 2 cut(s) 140, 483
BseCI ATCGAT 2 cut(s) 65, 84
BseDI CCNNGG 1 cut(s) 482
BseLI CCNNNNNNNGG 1 cut(s) 517
BseMII CTCAG 3 cut(s) 117, 153, 230
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 4 cut(s) 278, 451, 552, 716
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 4 cut(s) 221, 481, 487, 619
BshNI GGYRCC 1 cut(s) 319
BshVI ATCGAT 2 cut(s) 65, 84
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 2 cut(s) 20, 78
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 517
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 4 cut(s) 221, 481, 487, 619
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 5 cut(s) 62, 66, 85, 298, 649
BspACI CCGC 3 cut(s) 488, 620, 707
BspANI GGCC 4 cut(s) 221, 481, 487, 619
BspCNI CTCAG 3 cut(s) 116, 154, 229
BspDI ATCGAT 2 cut(s) 65, 84
BspLI GGNNCC 1 cut(s) 321
BspOI GCTAGC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 319
BsrBI CCGCTC 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 5 cut(s) 62, 66, 85, 298, 649
BssNI GRCGYC 1 cut(s) 528
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 2 cut(s) 140, 483
Bst4CI ACNGT 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 528
BstC8I GCNNGC 2 cut(s) 79, 462
BstDEI CTNAG 3 cut(s) 103, 162, 216
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 5 cut(s) 65, 69, 88, 301, 652
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 5 cut(s) 62, 66, 85, 298, 649
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 3 cut(s) 478, 487, 628
BstNI CCWGG 2 cut(s) 140, 483
BstSCI CCNGG 2 cut(s) 138, 481
BstV1I GCAGC 4 cut(s) 278, 451, 552, 716
Bsu15I ATCGAT 2 cut(s) 65, 84
BsuRI GGCC 4 cut(s) 221, 481, 487, 619
BsuTUI ATCGAT 2 cut(s) 65, 84
BtrI CACGTC 1 cut(s) 504
BtsIMutI CAGTG 2 cut(s) 199, 294
Cac8I GCNNGC 2 cut(s) 79, 462
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 485
ClaI ATCGAT 2 cut(s) 65, 84
CseI GACGC 2 cut(s) 466, 517
Csp6I GTAC 1 cut(s) 11
CviAII CATG 5 cut(s) 29, 284, 469, 569, 575
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 3 cut(s) 103, 162, 216
DpnI GATC 5 cut(s) 64, 68, 87, 300, 651
DpnII GATC 5 cut(s) 62, 66, 85, 298, 649
DriI GACNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 617
Eam1105I GACNNNNNGTC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 633
Eco53kI GAGCTC 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 631
EcoRI GAATTC 2 cut(s) 35, 410
EcoRII CCWGG 2 cut(s) 138, 481
EcoT38I GRGCYC 1 cut(s) 633
FaeI CATG 5 cut(s) 32, 287, 472, 572, 578
FaqI GGGAC 1 cut(s) 201
FatI CATG 5 cut(s) 28, 283, 468, 568, 574
FbaI TGATCA 2 cut(s) 298, 649
Fnu4HI GCNGC 7 cut(s) 267, 465, 488, 566, 620, 705, 708
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 7 cut(s) 267, 465, 488, 566, 620, 705, 708
FspBI CTAG 1 cut(s) 461
GlaI GCGC 1 cut(s) 394
GluI GCNGC 7 cut(s) 267, 465, 488, 566, 620, 705, 708
GsuI CTGGAG 1 cut(s) 219
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 4 cut(s) 221, 481, 487, 619
HapII CCGG 2 cut(s) 20, 78
HgaI GACGC 2 cut(s) 466, 517
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 5 cut(s) 32, 287, 472, 572, 578
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HinfI GANTC 2 cut(s) 439, 452
HpaII CCGG 2 cut(s) 20, 78
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 553, 722
Hpy188I TCNGA 3 cut(s) 94, 334, 628
Hpy188III TCNNGA 5 cut(s) 70, 302, 403, 446, 580
Hpy8I GTNNAC 2 cut(s) 553, 722
Hpy99I CGWCG 1 cut(s) 460
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 2 cut(s) 503, 555
HpyCH4V TGCA 3 cut(s) 266, 568, 643
HpyF10VI GCNNNNNNNGC 3 cut(s) 478, 487, 628
HpyF3I CTNAG 3 cut(s) 103, 162, 216
HpySE526I ACGT 2 cut(s) 503, 555
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 5 cut(s) 32, 287, 472, 572, 578
HspAI GCGC 1 cut(s) 393
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Ksp22I TGATCA 2 cut(s) 298, 649
Kzo9I GATC 5 cut(s) 62, 66, 85, 298, 649
LmnI GCTCC 2 cut(s) 371, 628
Lsp1109I GCAGC 4 cut(s) 278, 451, 552, 716
LweI GCATC 2 cut(s) 85, 90
MaeI CTAG 1 cut(s) 461
MaeII ACGT 2 cut(s) 503, 555
MaeIII GTNAC 1 cut(s) 211
MalI GATC 5 cut(s) 64, 68, 87, 300, 651
MbiI CCGCTC 1 cut(s) 490
MboI GATC 5 cut(s) 62, 66, 85, 298, 649
MfeI CAATTG 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 5 cut(s) 35, 177, 243, 410, 434
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 410, 439
MnlI CCTC 8 cut(s) 194, 393, 442, 453, 471, 511, 514, 606
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 2 cut(s) 279, 558
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 707
MspI CCGG 2 cut(s) 20, 78
MspR9I CCNGG 2 cut(s) 140, 483
MunI CAATTG 1 cut(s) 434
MvaI CCWGG 2 cut(s) 140, 483
MwoI GCNNNNNNNGC 3 cut(s) 478, 487, 628
NaeI GCCGGC 1 cut(s) 79
NdeII GATC 5 cut(s) 62, 66, 85, 298, 649
NgoMIV GCCGGC 1 cut(s) 77
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 5 cut(s) 32, 287, 472, 572, 578
NlaIV GGNNCC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 2 cut(s) 527, 575
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 7 cut(s) 268, 466, 489, 567, 621, 706, 709
Ple19I CGATCG 1 cut(s) 65
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 138, 481
PspGI CCWGG 2 cut(s) 138, 481
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 485
PsyI GACNNNGTC 2 cut(s) 527, 575
PvuI CGATCG 1 cut(s) 65
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 573
SacI GAGCTC 1 cut(s) 633
SaqAI TTAA 2 cut(s) 279, 558
SatI GCNGC 7 cut(s) 267, 465, 488, 566, 620, 705, 708
Sau3AI GATC 5 cut(s) 62, 66, 85, 298, 649
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 2 cut(s) 140, 483
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 2 cut(s) 85, 90
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 2 cut(s) 227, 444
SmoI CTYRAG 2 cut(s) 227, 444
Sse9I AATT 5 cut(s) 35, 177, 243, 410, 434
SsiI CCGC 3 cut(s) 488, 620, 707
SspMI CTAG 1 cut(s) 461
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 2 cut(s) 138, 481
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 2 cut(s) 506, 558
TaqI TCGA 7 cut(s) 61, 65, 84, 455, 516, 534, 602
TasI AATT 5 cut(s) 35, 177, 243, 410, 434
TauI GCSGC 3 cut(s) 490, 622, 710
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 2 cut(s) 279, 558
Tru9I TTAA 2 cut(s) 279, 558
TscAI CASTG 2 cut(s) 199, 301
TseI GCWGC 4 cut(s) 266, 464, 565, 704
TspDTI ATGAA 1 cut(s) 183
TspRI CASTG 2 cut(s) 199, 301
Tth111I GACNNNGTC 2 cut(s) 527, 575
XapI RAATTY 3 cut(s) 35, 243, 410
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.