MD07G1237000.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012379
Physical Location & Seq
Forward (+)
30966093 .. 30969218
3126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1237000.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGTAGAGGAGAAGCCTGAGGAAGAAACCATAGTAGAGGAGAAGATGGAATTATCAGAAGTACACTTGAAGACTGCTCATTTTGTGCTGGTTCACGGCATAAGTCATGGAGCATGGTGCTGGTACAAAATCCGGTGTCTCATGGAGAATTCCGGCTACAAGGTATCGTGTGTCGATCTCAAGAGCGCCGGCATCGATCAATCTGATGCGAACTCAGTCCTTTCTTTTGATGATTACAACAAGCCACTCCTGGACTTACTGTCTGCTCTCCCTGAGAATGAACAGGTAATTCTGGTGGGACACAGTGCTGGAGGGCTGAATGTAACTCAGGCCACTATCAAGTTCCCAAAGAAAATTCTCCTAGCAGTATATGTTGCAGCAACAATGCTCAAACATGGCTTCTCCACTGATCAAGATTTCAAAGATGGGGTGCCAGATTTATCAGAGTTTGGTGATGTATATGAGTTAGGGTTTGGATTGGGAGCTGATAAACCTCCAACAAGCGCCATTGTCAAGAAAGAATTCCAACGCAAAATCTCATACCAATTGAGTCCTCAAGAGGATTCGACGCTAGCTGCCATGCTTTTGAGGCCAGGGCCGCTCTTGGCAATCACGTCAGCCCATTTCGAAGAGGACGGCGTCATCGACCAAGTGCCACGAGTTTACGTTAAAACGCTGCATGACCATGTCGTGAAACCAGAGCAACAAGGTTCGATGGTAAAGAGGTGGCCGCCATCGGAGGTCTATGTTTTGCATAGTGATCACAGCCCTATGTTCTCCACCCCATTTCTGCTATTTGGCTTCCTTGTAAAAGCAGCGGCTTCTTATGGTGGACTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

281

Amino Acids

30.99

Weight (kDa)

5.44

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 26 - 129 2.7e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 27 - 257 1.3e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 28 - 262 7.5e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 601
AciI CCGC 3 cut(s) 599, 731, 818
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 3 cut(s) 148, 354, 521
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 2 cut(s) 63, 125
AgsI TTSAA 2 cut(s) 70, 421
AhdI GACNNNNNGTC 1 cut(s) 259
AjiI CACGTC 1 cut(s) 615
AjnI CCWGG 2 cut(s) 249, 592
AluBI AGCT 2 cut(s) 485, 575
AluI AGCT 2 cut(s) 485, 575
Alw26I GTCTC 1 cut(s) 143
AoxI GGCC 4 cut(s) 330, 590, 596, 728
ApeKI GCWGC 4 cut(s) 377, 575, 676, 815
ApoI RAATTY 3 cut(s) 148, 354, 521
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 2 cut(s) 188, 506
AspS9I GGNCC 1 cut(s) 596
AsuHPI GGTGA 1 cut(s) 464
AsuII TTCGAA 1 cut(s) 627
AsuNHI GCTAGC 1 cut(s) 571
AxyI CCTNAGG 1 cut(s) 18
BanI GGYRCC 1 cut(s) 430
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 4 cut(s) 389, 562, 663, 827
BccI CCATC 4 cut(s) 40, 419, 709, 742
BceAI ACGGC 2 cut(s) 112, 652
BciT130I CCWGG 2 cut(s) 251, 594
BclI TGATCA 2 cut(s) 409, 760
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 2 cut(s) 362, 572
BfoI RGCGCY 2 cut(s) 189, 507
BisI GCNGC 7 cut(s) 378, 576, 599, 677, 731, 816, 819
BlsI GCNGC 7 cut(s) 379, 577, 600, 678, 732, 817, 820
Bme1390I CCNGG 2 cut(s) 251, 594
BmeRI GACNNNNNGTC 1 cut(s) 259
BmgBI CACGTC 1 cut(s) 615
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 2 cut(s) 251, 594
BmsI GCATC 2 cut(s) 196, 201
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 330
Bpu14I TTCGAA 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 164, 540
Bsa29I ATCGAT 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 639
BsaJI CCNNGG 1 cut(s) 593
BsaWI WCCGGW 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 188
Bse21I CCTNAGG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 251, 594
BseCI ATCGAT 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 593
BseMII CTCAG 4 cut(s) 9, 228, 264, 341
BseRI GAGGAG 2 cut(s) 23, 53
BseXI GCAGC 4 cut(s) 389, 562, 663, 827
BshFI GGCC 4 cut(s) 332, 592, 598, 730
BshNI GGYRCC 1 cut(s) 430
BshVI ATCGAT 1 cut(s) 195
BsiSI CCGG 3 cut(s) 133, 153, 189
BslFI GGGAC 1 cut(s) 312
BsmAI GTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 4 cut(s) 332, 592, 598, 730
Bsp119I TTCGAA 1 cut(s) 627
Bsp143I GATC 4 cut(s) 175, 196, 409, 760
BspACI CCGC 3 cut(s) 599, 731, 818
BspANI GGCC 4 cut(s) 332, 592, 598, 730
BspCNI CTCAG 4 cut(s) 10, 227, 265, 340
BspDI ATCGAT 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 432
BspOI GCTAGC 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 430
BsrBI CCGCTC 1 cut(s) 601
BsrFI RCCGGY 1 cut(s) 188
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 593
BssMI GATC 4 cut(s) 175, 196, 409, 760
BssNI GRCGYC 1 cut(s) 639
BssSI CACGAG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 251, 594
Bst4CI ACNGT 2 cut(s) 261, 305
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 639
BstBI TTCGAA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 190, 573
BstDEI CTNAG 4 cut(s) 18, 214, 273, 327
BstH2I RGCGCY 2 cut(s) 189, 507
BstHHI GCGC 2 cut(s) 188, 506
BstKTI GATC 4 cut(s) 178, 199, 412, 763
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 4 cut(s) 175, 196, 409, 760
BstMWI GCNNNNNNNGC 2 cut(s) 589, 598
BstNI CCWGG 2 cut(s) 251, 594
BstSCI CCNGG 2 cut(s) 249, 592
BstV1I GCAGC 4 cut(s) 389, 562, 663, 827
BstV2I GAAGAC 1 cut(s) 77
Bsu15I ATCGAT 1 cut(s) 195
Bsu36I CCTNAGG 1 cut(s) 18
BsuRI GGCC 4 cut(s) 332, 592, 598, 730
BsuTUI ATCGAT 1 cut(s) 195
BtrI CACGTC 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 310, 405
Cac8I GCNNGC 2 cut(s) 190, 573
CfoI GCGC 2 cut(s) 188, 506
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 596
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 2 cut(s) 577, 628
Csp6I GTAC 2 cut(s) 62, 124
CviAII CATG 7 cut(s) 107, 114, 142, 395, 580, 680, 686
CviQI GTAC 2 cut(s) 62, 124
DdeI CTNAG 4 cut(s) 18, 214, 273, 327
DpnI GATC 4 cut(s) 177, 198, 411, 762
DpnII GATC 4 cut(s) 175, 196, 409, 760
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 728
Eam1104I CTCTTC 1 cut(s) 624
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 1 cut(s) 624
Eco81I CCTNAGG 1 cut(s) 18
EcoRI GAATTC 2 cut(s) 148, 521
EcoRII CCWGG 2 cut(s) 249, 592
FaeI CATG 7 cut(s) 110, 117, 145, 398, 583, 683, 689
FaqI GGGAC 1 cut(s) 312
FatI CATG 7 cut(s) 106, 113, 141, 394, 579, 679, 685
FbaI TGATCA 2 cut(s) 409, 760
Fnu4HI GCNGC 7 cut(s) 378, 576, 599, 677, 731, 816, 819
Fsp4HI GCNGC 7 cut(s) 378, 576, 599, 677, 731, 816, 819
FspBI CTAG 2 cut(s) 362, 572
GlaI GCGC 2 cut(s) 187, 505
GluI GCNGC 7 cut(s) 378, 576, 599, 677, 731, 816, 819
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 2 cut(s) 189, 507
HaeIII GGCC 4 cut(s) 332, 592, 598, 730
HapII CCGG 3 cut(s) 133, 153, 189
HgaI GACGC 2 cut(s) 577, 628
HhaI GCGC 2 cut(s) 188, 506
Hin1I GRCGYC 1 cut(s) 639
Hin1II CATG 7 cut(s) 110, 117, 145, 398, 583, 683, 689
Hin6I GCGC 2 cut(s) 186, 504
HinP1I GCGC 2 cut(s) 186, 504
HinfI GANTC 2 cut(s) 550, 563
HpaII CCGG 3 cut(s) 133, 153, 189
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 4 cut(s) 64, 94, 664, 833
Hpy188I TCNGA 4 cut(s) 58, 205, 445, 739
Hpy188III TCNNGA 5 cut(s) 181, 413, 514, 557, 691
Hpy8I GTNNAC 4 cut(s) 64, 94, 664, 833
Hpy99I CGWCG 1 cut(s) 571
HpyCH4III ACNGT 2 cut(s) 261, 305
HpyCH4IV ACGT 2 cut(s) 614, 666
HpyCH4V TGCA 3 cut(s) 377, 679, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 589, 598
HpyF3I CTNAG 4 cut(s) 18, 214, 273, 327
HpySE526I ACGT 2 cut(s) 614, 666
Hsp92I GRCGYC 1 cut(s) 639
Hsp92II CATG 7 cut(s) 110, 117, 145, 398, 583, 683, 689
HspAI GCGC 2 cut(s) 186, 504
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 2 cut(s) 409, 760
Kzo9I GATC 4 cut(s) 175, 196, 409, 760
LmnI GCTCC 2 cut(s) 110, 482
Lsp1109I GCAGC 4 cut(s) 389, 562, 663, 827
LweI GCATC 2 cut(s) 196, 201
MaeI CTAG 2 cut(s) 362, 572
MaeII ACGT 2 cut(s) 614, 666
MaeIII GTNAC 1 cut(s) 322
MalI GATC 4 cut(s) 177, 198, 411, 762
MbiI CCGCTC 1 cut(s) 601
MboI GATC 4 cut(s) 175, 196, 409, 760
MboII GAAGA 4 cut(s) 35, 55, 82, 641
MfeI CAATTG 1 cut(s) 545
MluCI AATT 6 cut(s) 50, 148, 288, 354, 521, 545
MlyI GAGTC 1 cut(s) 559
MmeI TCCRAC 2 cut(s) 521, 550
MroNI GCCGGC 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 2 cut(s) 669, 837
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 3 cut(s) 133, 153, 189
MspR9I CCNGG 2 cut(s) 251, 594
MunI CAATTG 1 cut(s) 545
MvaI CCWGG 2 cut(s) 251, 594
MwoI GCNNNNNNNGC 2 cut(s) 589, 598
NaeI GCCGGC 1 cut(s) 190
NdeII GATC 4 cut(s) 175, 196, 409, 760
NgoMIV GCCGGC 1 cut(s) 188
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 7 cut(s) 110, 117, 145, 398, 583, 683, 689
NlaIV GGNNCC 1 cut(s) 432
NspV TTCGAA 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PdiI GCCGGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 563
PflFI GACNNNGTC 2 cut(s) 638, 686
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 7 cut(s) 379, 577, 600, 678, 732, 817, 820
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
Psp6I CCWGG 2 cut(s) 249, 592
PspGI CCWGG 2 cut(s) 249, 592
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 1 cut(s) 596
PsyI GACNNNGTC 2 cut(s) 638, 686
RsaI GTAC 2 cut(s) 63, 125
RsaNI GTAC 2 cut(s) 62, 124
RseI CAYNNNNRTG 1 cut(s) 684
SaqAI TTAA 2 cut(s) 669, 837
SatI GCNGC 7 cut(s) 378, 576, 599, 677, 731, 816, 819
Sau3AI GATC 4 cut(s) 175, 196, 409, 760
Sau96I GGNCC 1 cut(s) 596
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 2 cut(s) 251, 594
SfaNI GCATC 2 cut(s) 196, 201
SfuI TTCGAA 1 cut(s) 627
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 179, 555
SmoI CTYRAG 2 cut(s) 179, 555
Sse9I AATT 6 cut(s) 50, 148, 288, 354, 521, 545
SsiI CCGC 3 cut(s) 599, 731, 818
SspMI CTAG 2 cut(s) 362, 572
StyD4I CCNGG 2 cut(s) 249, 592
TaaI ACNGT 2 cut(s) 261, 305
TaiI ACGT 2 cut(s) 617, 669
TaqI TCGA 6 cut(s) 174, 195, 566, 627, 645, 713
TasI AATT 6 cut(s) 50, 148, 288, 354, 521, 545
TatI WGTACW 1 cut(s) 61
TauI GCSGC 3 cut(s) 601, 733, 821
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 2 cut(s) 669, 837
Tru9I TTAA 2 cut(s) 669, 837
TscAI CASTG 2 cut(s) 310, 412
TseI GCWGC 4 cut(s) 377, 575, 676, 815
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 2 cut(s) 310, 412
Tth111I GACNNNGTC 2 cut(s) 638, 686
XapI RAATTY 3 cut(s) 148, 354, 521
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 2 cut(s) 362, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.