MD01G1170100.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012379
Physical Location & Seq
Forward (+)
27365107 .. 27368239
3133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1170100.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGTAGAGGATAAGGCTACGGAAGAAACCATGGTAGATGAGGAGAAGGAAATATCATCAGAACCCTTGAAGGCTACTACTCACTTTGTGCTGGTCCATGGCATAAGTCACGGGGCATGGTGTTGGTACAAAATCCGGTGTCTCATGGAGAGTTCTGGCTTCAAAGTTTCGTGTGTCGATCTGAAGAGCGCCGGCCTCGATCAATCCGATGCAAATTCAGTCCTTTCTTTTGATGATTACAACAAGCCACTCCTGGACTTGCTGTCTGCCCTCCCTGAGAATGAAAAGGTAATTCTGGTGGGACACAGTGCTGGAGGGCTGAATGTAACTCAGGCCACTCTCAAGTTCCCAAAGAAAATTCTCTTAGCAGTCTATGTTGCAGCGACAATGCTCAAACTTGGGTTCTCCAGTGATCAAGATTTTAAAGATGGGGTGCCAGATTTATCTGAGTTTGGTGATGTGTATGAGTTAGGGTTTGGATTGGGATCTGATAAACCTCCAACAAGCGCCATTGTCAAGAAAGAATTCCAACGCAAAATCTCATACCAATTGAGTCCTCAAGAGGATTCGACGCTAGCTGCTATGCTTTTGAGGCCAGGACCGCTCTTGGCAATAACGTCAGCCCGTTTCGCAGAGGACAGCATCGTTGACAAAGTGCCGCGAGTTTACTTTAGAACGCTGCATGACCATGTCATAAACCCAGGGCAACAAGAAGCTATGGTAAAGAGGTGGCCACCGTCGGAGGTCTATGTTTTGGATAGTGATCACAGCCCCATGTTCTCCACCCCATTTTTGCTGTTTGGCTTCCTTGTAAAAGCAGCGGCTTCTTTTGGTGGACTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

282

Amino Acids

30.92

Weight (kDa)

5.3

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 28 - 259 4.5e-16 alpha/beta hydrolase fold
Hydrolase_4 PF12146 28 - 132 1.2e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 29 - 263 3.8e-17 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 604
AccII CGCG 1 cut(s) 661
AciI CCGC 3 cut(s) 602, 659, 821
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 3 cut(s) 214, 357, 524
AcuI CTGAAG 1 cut(s) 203
AdeI CACNNNGTG 1 cut(s) 88
AfaI GTAC 1 cut(s) 128
AgsI TTSAA 2 cut(s) 70, 163
AhdI GACNNNNNGTC 1 cut(s) 262
AjnI CCWGG 3 cut(s) 252, 595, 700
AluBI AGCT 2 cut(s) 578, 716
AluI AGCT 2 cut(s) 578, 716
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 1 cut(s) 493
AoxI GGCC 4 cut(s) 193, 333, 593, 731
ApeKI GCWGC 4 cut(s) 380, 578, 679, 818
ApoI RAATTY 3 cut(s) 214, 357, 524
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 2 cut(s) 191, 509
AspS9I GGNCC 2 cut(s) 94, 599
AsuHPI GGTGA 1 cut(s) 467
AsuNHI GCTAGC 1 cut(s) 574
AvaII GGWCC 2 cut(s) 94, 599
BalI TGGCCA 1 cut(s) 733
BanI GGYRCC 1 cut(s) 433
BbvI GCAGC 4 cut(s) 392, 565, 666, 830
BccI CCATC 1 cut(s) 422
BciT130I CCWGG 3 cut(s) 254, 597, 702
BclI TGATCA 2 cut(s) 412, 763
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 575
BfoI RGCGCY 2 cut(s) 192, 510
BisI GCNGC 6 cut(s) 381, 579, 659, 680, 819, 822
BlsI GCNGC 6 cut(s) 382, 580, 660, 681, 820, 823
Bme1390I CCNGG 3 cut(s) 254, 597, 702
Bme18I GGWCC 2 cut(s) 94, 599
BmeRI GACNNNNNGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 94, 599
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 254, 597, 702
BmsI GCATC 2 cut(s) 199, 651
BmtI GCTAGC 1 cut(s) 578
BpmI CTGGAG 2 cut(s) 333, 391
BpuEI CTTGAG 2 cut(s) 326, 543
BsaBI GATNNNNATC 2 cut(s) 484, 762
BsaJI CCNNGG 4 cut(s) 30, 97, 700, 701
BsaWI WCCGGW 1 cut(s) 135
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 408
Bse8I GATNNNNATC 2 cut(s) 484, 762
BseBI CCWGG 3 cut(s) 254, 597, 702
BseDI CCNNGG 4 cut(s) 30, 97, 700, 701
BseJI GATNNNNATC 2 cut(s) 484, 762
BseMII CTCAG 3 cut(s) 267, 344, 438
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 4 cut(s) 392, 565, 666, 830
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 4 cut(s) 195, 335, 595, 733
BshNI GGYRCC 1 cut(s) 433
BsiSI CCGG 2 cut(s) 136, 192
BslFI GGGAC 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 4 cut(s) 195, 335, 595, 733
Bsp143I GATC 5 cut(s) 178, 199, 412, 485, 763
Bsp19I CCATGG 2 cut(s) 30, 97
BspACI CCGC 3 cut(s) 602, 659, 821
BspANI GGCC 4 cut(s) 195, 335, 595, 733
BspCNI CTCAG 3 cut(s) 268, 343, 439
BspFNI CGCG 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 435
BspOI GCTAGC 1 cut(s) 578
BspPI GGATC 1 cut(s) 493
BspQI GCTCTTC 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 433
BsrBI CCGCTC 1 cut(s) 604
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 4 cut(s) 30, 97, 700, 701
BssMI GATC 5 cut(s) 178, 199, 412, 485, 763
BssT1I CCWWGG 2 cut(s) 30, 97
Bst2UI CCWGG 3 cut(s) 254, 597, 702
Bst4CI ACNGT 2 cut(s) 308, 738
Bst6I CTCTTC 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 193, 576
BstDEI CTNAG 4 cut(s) 276, 330, 364, 447
BstDSI CCRYGG 2 cut(s) 30, 97
BstFNI CGCG 1 cut(s) 661
BstH2I RGCGCY 2 cut(s) 192, 510
BstHHI GCGC 2 cut(s) 191, 509
BstKTI GATC 5 cut(s) 181, 202, 415, 488, 766
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 5 cut(s) 178, 199, 412, 485, 763
BstMWI GCNNNNNNNGC 3 cut(s) 592, 601, 629
BstNI CCWGG 3 cut(s) 254, 597, 702
BstSCI CCNGG 3 cut(s) 252, 595, 700
BstUI CGCG 1 cut(s) 661
BstV1I GCAGC 4 cut(s) 392, 565, 666, 830
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuRI GGCC 4 cut(s) 195, 335, 595, 733
BtgI CCRYGG 2 cut(s) 30, 97
BtsIMutI CAGTG 2 cut(s) 313, 415
Cac8I GCNNGC 2 cut(s) 193, 576
CfoI GCGC 2 cut(s) 191, 509
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 2 cut(s) 94, 599
CseI GACGC 1 cut(s) 580
Csp6I GTAC 1 cut(s) 127
CviAII CATG 7 cut(s) 31, 98, 117, 145, 683, 689, 775
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 4 cut(s) 276, 330, 364, 447
DpnI GATC 5 cut(s) 180, 201, 414, 487, 765
DpnII GATC 5 cut(s) 178, 199, 412, 485, 763
DraI TTTAAA 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 88
DriI GACNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 179
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 179
Eco130I CCWWGG 2 cut(s) 30, 97
Eco47I GGWCC 2 cut(s) 94, 599
Eco57I CTGAAG 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 3 cut(s) 252, 595, 700
EcoT14I CCWWGG 2 cut(s) 30, 97
ErhI CCWWGG 2 cut(s) 30, 97
FaeI CATG 7 cut(s) 34, 101, 120, 148, 686, 692, 778
FaqI GGGAC 1 cut(s) 315
FatI CATG 7 cut(s) 30, 97, 116, 144, 682, 688, 774
FbaI TGATCA 2 cut(s) 412, 763
Fnu4HI GCNGC 6 cut(s) 381, 579, 659, 680, 819, 822
Fsp4HI GCNGC 6 cut(s) 381, 579, 659, 680, 819, 822
FspBI CTAG 1 cut(s) 575
GlaI GCGC 2 cut(s) 190, 508
GluI GCNGC 6 cut(s) 381, 579, 659, 680, 819, 822
GsuI CTGGAG 2 cut(s) 333, 391
HaeII RGCGCY 2 cut(s) 192, 510
HaeIII GGCC 4 cut(s) 195, 335, 595, 733
HapII CCGG 2 cut(s) 136, 192
HgaI GACGC 1 cut(s) 580
HhaI GCGC 2 cut(s) 191, 509
Hin1II CATG 7 cut(s) 34, 101, 120, 148, 686, 692, 778
Hin6I GCGC 2 cut(s) 189, 507
HinP1I GCGC 2 cut(s) 189, 507
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 2 cut(s) 553, 566
HpaII CCGG 2 cut(s) 136, 192
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 3 cut(s) 649, 667, 836
Hpy188I TCNGA 6 cut(s) 61, 183, 208, 448, 490, 742
Hpy188III TCNNGA 3 cut(s) 416, 517, 560
Hpy8I GTNNAC 3 cut(s) 649, 667, 836
Hpy99I CGWCG 2 cut(s) 574, 742
HpyAV CCTTC 2 cut(s) 40, 64
HpyCH4III ACNGT 2 cut(s) 308, 738
HpyCH4IV ACGT 1 cut(s) 617
HpyCH4V TGCA 3 cut(s) 212, 380, 682
HpyF10VI GCNNNNNNNGC 3 cut(s) 592, 601, 629
HpyF3I CTNAG 4 cut(s) 276, 330, 364, 447
HpySE526I ACGT 1 cut(s) 617
Hsp92II CATG 7 cut(s) 34, 101, 120, 148, 686, 692, 778
HspAI GCGC 2 cut(s) 189, 507
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Ksp22I TGATCA 2 cut(s) 412, 763
Kzo9I GATC 5 cut(s) 178, 199, 412, 485, 763
LguI GCTCTTC 1 cut(s) 179
Lsp1109I GCAGC 4 cut(s) 392, 565, 666, 830
LweI GCATC 2 cut(s) 199, 651
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 2 cut(s) 107, 325
MalI GATC 5 cut(s) 180, 201, 414, 487, 765
MbiI CCGCTC 1 cut(s) 604
MboI GATC 5 cut(s) 178, 199, 412, 485, 763
MboII GAAGA 2 cut(s) 35, 196
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 733
MluCI AATT 5 cut(s) 214, 291, 357, 524, 548
MluNI TGGCCA 1 cut(s) 733
MlyI GAGTC 1 cut(s) 562
MmeI TCCRAC 3 cut(s) 524, 553, 720
Mox20I TGGCCA 1 cut(s) 733
MroNI GCCGGC 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 524
MscI TGGCCA 1 cut(s) 733
MseI TTAA 2 cut(s) 423, 840
MslI CAYNNNNRTG 1 cut(s) 687
Msp20I TGGCCA 1 cut(s) 733
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 2 cut(s) 136, 192
MspR9I CCNGG 3 cut(s) 254, 597, 702
MunI CAATTG 1 cut(s) 548
MvaI CCWGG 3 cut(s) 254, 597, 702
MvnI CGCG 1 cut(s) 661
MwoI GCNNNNNNNGC 3 cut(s) 592, 601, 629
NaeI GCCGGC 1 cut(s) 193
NcoI CCATGG 2 cut(s) 30, 97
NdeII GATC 5 cut(s) 178, 199, 412, 485, 763
NgoMIV GCCGGC 1 cut(s) 191
NheI GCTAGC 1 cut(s) 574
NlaIII CATG 7 cut(s) 34, 101, 120, 148, 686, 692, 778
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 107
PasI CCCWGGG 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdiI GCCGGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 1 cut(s) 566
PflFI GACNNNGTC 1 cut(s) 689
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 6 cut(s) 382, 580, 660, 681, 820, 823
PleI GAGTC 1 cut(s) 561
PpsI GAGTC 1 cut(s) 561
Psp6I CCWGG 3 cut(s) 252, 595, 700
PspGI CCWGG 3 cut(s) 252, 595, 700
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 2 cut(s) 94, 599
PsuI RGATCY 1 cut(s) 485
PsyI GACNNNGTC 1 cut(s) 689
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 179
SaqAI TTAA 2 cut(s) 423, 840
SatI GCNGC 6 cut(s) 381, 579, 659, 680, 819, 822
Sau3AI GATC 5 cut(s) 178, 199, 412, 485, 763
Sau96I GGNCC 2 cut(s) 94, 599
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 3 cut(s) 254, 597, 702
SetI ASST 7 cut(s) 291, 499, 580, 620, 718, 731, 747
SfaNI GCATC 2 cut(s) 199, 651
SinI GGWCC 2 cut(s) 94, 599
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 341, 558
SmoI CTYRAG 2 cut(s) 341, 558
Sse9I AATT 5 cut(s) 214, 291, 357, 524, 548
SsiI CCGC 3 cut(s) 602, 659, 821
SspMI CTAG 1 cut(s) 575
StyD4I CCNGG 3 cut(s) 252, 595, 700
StyI CCWWGG 2 cut(s) 30, 97
TaaI ACNGT 2 cut(s) 308, 738
TaiI ACGT 1 cut(s) 620
TaqI TCGA 3 cut(s) 177, 198, 569
TasI AATT 5 cut(s) 214, 291, 357, 524, 548
TauI GCSGC 2 cut(s) 661, 824
TfiI GAWTC 1 cut(s) 566
Tru1I TTAA 2 cut(s) 423, 840
Tru9I TTAA 2 cut(s) 423, 840
TscAI CASTG 2 cut(s) 313, 415
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 4 cut(s) 380, 578, 679, 818
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 1 cut(s) 297
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 313, 415
Tth111I GACNNNGTC 1 cut(s) 689
VpaK11BI GGWCC 2 cut(s) 94, 599
XapI RAATTY 3 cut(s) 214, 357, 524
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.