pycom01g18280

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012379
Physical Location & Seq
Forward (+)
17321065 .. 17323617
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18280.1

Sequence Viewer

Length: 984 bp
ATGATTAGTTTGGGGCATTGTTATCTTGGTGTTTTCGTTTTATGTAAATCATTATCTTATTATCTTAGAGAGACGGGCTCCGGGAAAGAGGAAAATAAAAGAGCTTTGGAGAGCAGTGGCTTTTGGGAAGCAGAAAGCATGGTAGAGGAGAAGGCTACGGAAGAAACCGTGGTAGATGAGGACAAGGAACTATCAGAAGAACCCTTGGGGGCTACTACTCACTTTGTGCTGGTCCATGGCATAAGTCACGGGGCATGGTGTTGGTACAAAATCCGGTGTCTCATGGAGAGTTCCGGCTACAAAGTTTCGTGTGTCGATCTGAAGAGCGCCGGCGTCGATCAATCCGATGCAAATTCAGTCCTATCTTTTGATGATTACAACAAGCCACTCCTGGACTTGTTGTCTGCTCTCGCTGAGAATGAAAAGGTAATTCTGGTGGGACACAGTGCTGGAGGGCTGAATGTAACTCAGGCCACTCTCAAGTTCCCAAAGAAAATTCTCTTAGCAGTCTATGTTGCAGCCACAATGCTCAAACTTGGGTTCTCCAGTGATCAAGATTTTAAAGATGGGGTGCCAGATTTATCTGAGTTTGGTGATGTGTATGAGTTAGGGTTTGGATTGGGATCTGATAAACCTCCAACAAGCGCCATTGTCAAGAAAGAATTCCAACGCAAAATCTCATACCAATTGAGTCCTCAAGAGGATTCGACGCTAGCTGCTATGCTTTTGAGGCCAGGACCGCTCTTGGCAATAACGTCAGCCCGTTTCGCAGAGGACAGCGTCGTTGACAAAGTGCCGCGAGTTTACCTTAGAACGCTGCATGACCATGTCGTAAAACCAGGGCAACAAGAAGCGATGGTAAAGAGGTGGCCACCGTCGGAGGTCTATGTTTTGGATAGTGATCACAGCCCCATGTTCTCCACCCCATTTTTGCTGTTTGGCTTCCTTGTAAAAGCAGCGGCTTCTTTTGGTGGACTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

328

Amino Acids

35.95

Weight (kDa)

5.27

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 74 - 305 3.3e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 75 - 309 6.7e-18 Alpha/beta hydrolase family
Hydrolase_4 PF12146 75 - 178 1.8e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 571
AccBSI CCGCTC 1 cut(s) 742
AccII CGCG 1 cut(s) 799
AciI CCGC 3 cut(s) 740, 797, 959
AclWI GGATC 1 cut(s) 631
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 3 cut(s) 352, 495, 662
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 226
AfaI GTAC 1 cut(s) 266
AhdI GACNNNNNGTC 1 cut(s) 400
AjnI CCWGG 3 cut(s) 390, 733, 838
AluBI AGCT 2 cut(s) 104, 716
AluI AGCT 2 cut(s) 104, 716
Alw26I GTCTC 2 cut(s) 65, 284
AlwI GGATC 1 cut(s) 631
AoxI GGCC 3 cut(s) 471, 731, 869
ApeKI GCWGC 4 cut(s) 518, 716, 817, 956
ApoI RAATTY 3 cut(s) 352, 495, 662
Asp700I GAANNNNTTC 1 cut(s) 662
AspLEI GCGC 2 cut(s) 329, 647
AspS9I GGNCC 2 cut(s) 232, 737
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 605
AsuNHI GCTAGC 1 cut(s) 712
AvaII GGWCC 2 cut(s) 232, 737
BalI TGGCCA 1 cut(s) 871
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 80
BbvI GCAGC 4 cut(s) 530, 703, 804, 968
BccI CCATC 2 cut(s) 560, 850
BciT130I CCWGG 3 cut(s) 392, 735, 840
BclI TGATCA 2 cut(s) 550, 901
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 2 cut(s) 65, 284
BfaI CTAG 1 cut(s) 713
BfoI RGCGCY 2 cut(s) 330, 648
BisI GCNGC 6 cut(s) 519, 717, 797, 818, 957, 960
BlsI GCNGC 6 cut(s) 520, 718, 798, 819, 958, 961
Bme1390I CCNGG 4 cut(s) 82, 392, 735, 840
Bme18I GGWCC 2 cut(s) 232, 737
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 232, 737
BmiI GGNNCC 2 cut(s) 79, 573
BmrFI CCNGG 4 cut(s) 82, 392, 735, 840
BmsI GCATC 1 cut(s) 337
BmtI GCTAGC 1 cut(s) 716
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 2 cut(s) 471, 529
BpuEI CTTGAG 2 cut(s) 464, 681
BpuMI CCSGG 1 cut(s) 82
BsaBI GATNNNNATC 2 cut(s) 622, 900
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 168, 204, 235, 839
BsaWI WCCGGW 1 cut(s) 273
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 546
Bse8I GATNNNNATC 2 cut(s) 622, 900
BseBI CCWGG 3 cut(s) 392, 735, 840
BseDI CCNNGG 4 cut(s) 168, 204, 235, 839
BseJI GATNNNNATC 2 cut(s) 622, 900
BseMII CTCAG 3 cut(s) 405, 482, 576
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 530, 703, 804, 968
Bsh1236I CGCG 1 cut(s) 799
BshFI GGCC 3 cut(s) 473, 733, 871
BshNI GGYRCC 1 cut(s) 571
BsiSI CCGG 4 cut(s) 81, 274, 294, 330
BslFI GGGAC 1 cut(s) 453
BsmAI GTCTC 2 cut(s) 65, 284
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 453
BsnI GGCC 3 cut(s) 473, 733, 871
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 5 cut(s) 316, 337, 550, 623, 901
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 3 cut(s) 740, 797, 959
BspANI GGCC 3 cut(s) 473, 733, 871
BspCNI CTCAG 3 cut(s) 406, 481, 577
BspFNI CGCG 1 cut(s) 799
BspLI GGNNCC 2 cut(s) 79, 573
BspOI GCTAGC 1 cut(s) 716
BspPI GGATC 1 cut(s) 631
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 571
BsrBI CCGCTC 1 cut(s) 742
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 168, 204, 235, 839
BssMI GATC 5 cut(s) 316, 337, 550, 623, 901
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 204, 235
Bst2UI CCWGG 3 cut(s) 392, 735, 840
Bst4CI ACNGT 3 cut(s) 169, 446, 876
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 331, 714
BstDEI CTNAG 6 cut(s) 65, 414, 468, 502, 585, 809
BstDSI CCRYGG 2 cut(s) 168, 235
BstFNI CGCG 1 cut(s) 799
BstH2I RGCGCY 2 cut(s) 330, 648
BstHHI GCGC 2 cut(s) 329, 647
BstKTI GATC 5 cut(s) 319, 340, 553, 626, 904
BstMAI GTCTC 2 cut(s) 65, 284
BstMBI GATC 5 cut(s) 316, 337, 550, 623, 901
BstMWI GCNNNNNNNGC 3 cut(s) 730, 739, 767
BstNI CCWGG 3 cut(s) 392, 735, 840
BstSCI CCNGG 4 cut(s) 80, 390, 733, 838
BstUI CGCG 1 cut(s) 799
BstV1I GCAGC 4 cut(s) 530, 703, 804, 968
BstX2I RGATCY 1 cut(s) 623
BstYI RGATCY 1 cut(s) 623
BsuRI GGCC 3 cut(s) 473, 733, 871
BtgI CCRYGG 2 cut(s) 168, 235
BtgZI GCGATG 1 cut(s) 869
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 3 cut(s) 121, 451, 553
Cac8I GCNNGC 2 cut(s) 331, 714
CfoI GCGC 2 cut(s) 329, 647
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 232, 737
CseI GACGC 3 cut(s) 322, 718, 769
Csp6I GTAC 1 cut(s) 265
CviAII CATG 7 cut(s) 139, 236, 255, 283, 821, 827, 913
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 6 cut(s) 65, 414, 468, 502, 585, 809
DpnI GATC 5 cut(s) 318, 339, 552, 625, 903
DpnII GATC 5 cut(s) 316, 337, 550, 623, 901
DraI TTTAAA 1 cut(s) 562
DraIII CACNNNGTG 1 cut(s) 226
DriI GACNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 1 cut(s) 317
Eam1105I GACNNNNNGTC 1 cut(s) 400
EarI CTCTTC 1 cut(s) 317
Eco130I CCWWGG 2 cut(s) 204, 235
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 232, 737
Eco57I CTGAAG 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 3 cut(s) 390, 733, 838
EcoT14I CCWWGG 2 cut(s) 204, 235
EcoT38I GRGCYC 1 cut(s) 80
ErhI CCWWGG 2 cut(s) 204, 235
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 7 cut(s) 142, 239, 258, 286, 824, 830, 916
FaqI GGGAC 1 cut(s) 453
FatI CATG 7 cut(s) 138, 235, 254, 282, 820, 826, 912
FbaI TGATCA 2 cut(s) 550, 901
Fnu4HI GCNGC 6 cut(s) 519, 717, 797, 818, 957, 960
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 6 cut(s) 519, 717, 797, 818, 957, 960
FspBI CTAG 1 cut(s) 713
GlaI GCGC 2 cut(s) 328, 646
GluI GCNGC 6 cut(s) 519, 717, 797, 818, 957, 960
GsuI CTGGAG 2 cut(s) 471, 529
HaeII RGCGCY 2 cut(s) 330, 648
HaeIII GGCC 3 cut(s) 473, 733, 871
HapII CCGG 4 cut(s) 81, 274, 294, 330
HgaI GACGC 3 cut(s) 322, 718, 769
HhaI GCGC 2 cut(s) 329, 647
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 7 cut(s) 142, 239, 258, 286, 824, 830, 916
Hin6I GCGC 2 cut(s) 327, 645
HinP1I GCGC 2 cut(s) 327, 645
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 2 cut(s) 691, 704
HpaII CCGG 4 cut(s) 81, 274, 294, 330
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 3 cut(s) 787, 805, 974
Hpy188I TCNGA 6 cut(s) 196, 321, 346, 586, 628, 880
Hpy188III TCNNGA 3 cut(s) 554, 655, 698
Hpy8I GTNNAC 3 cut(s) 787, 805, 974
Hpy99I CGWCG 4 cut(s) 338, 712, 785, 880
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 3 cut(s) 169, 446, 876
HpyCH4IV ACGT 1 cut(s) 755
HpyCH4V TGCA 3 cut(s) 350, 518, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 730, 739, 767
HpyF3I CTNAG 6 cut(s) 65, 414, 468, 502, 585, 809
HpySE526I ACGT 1 cut(s) 755
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 7 cut(s) 142, 239, 258, 286, 824, 830, 916
HspAI GCGC 2 cut(s) 327, 645
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Ksp22I TGATCA 2 cut(s) 550, 901
Kzo9I GATC 5 cut(s) 316, 337, 550, 623, 901
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 4 cut(s) 530, 703, 804, 968
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 713
MaeII ACGT 1 cut(s) 755
MaeIII GTNAC 2 cut(s) 245, 463
MalI GATC 5 cut(s) 318, 339, 552, 625, 903
MbiI CCGCTC 1 cut(s) 742
MboI GATC 5 cut(s) 316, 337, 550, 623, 901
MboII GAAGA 3 cut(s) 173, 209, 334
MfeI CAATTG 1 cut(s) 686
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 80
MlsI TGGCCA 1 cut(s) 871
MluCI AATT 5 cut(s) 352, 429, 495, 662, 686
MluNI TGGCCA 1 cut(s) 871
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 3 cut(s) 662, 691, 858
Mox20I TGGCCA 1 cut(s) 871
MreI CGCCGGCG 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 662
MscI TGGCCA 1 cut(s) 871
MseI TTAA 2 cut(s) 561, 978
MslI CAYNNNNRTG 1 cut(s) 825
Msp20I TGGCCA 1 cut(s) 871
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 4 cut(s) 81, 274, 294, 330
MspR9I CCNGG 4 cut(s) 82, 392, 735, 840
MunI CAATTG 1 cut(s) 686
MvaI CCWGG 3 cut(s) 392, 735, 840
MvnI CGCG 1 cut(s) 799
MwoI GCNNNNNNNGC 3 cut(s) 730, 739, 767
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 235
NdeII GATC 5 cut(s) 316, 337, 550, 623, 901
NgoMIV GCCGGC 1 cut(s) 329
NheI GCTAGC 1 cut(s) 712
NlaIII CATG 7 cut(s) 142, 239, 258, 286, 824, 830, 916
NlaIV GGNNCC 2 cut(s) 79, 573
NmuCI GTSAC 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PdiI GCCGGC 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 662
PfeI GAWTC 1 cut(s) 704
PflFI GACNNNGTC 2 cut(s) 779, 827
PfoI TCCNGGA 2 cut(s) 80, 390
PkrI GCNGC 6 cut(s) 520, 718, 798, 819, 958, 961
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
Psp6I CCWGG 3 cut(s) 390, 733, 838
PspGI CCWGG 3 cut(s) 390, 733, 838
PspN4I GGNNCC 2 cut(s) 79, 573
PspPI GGNCC 2 cut(s) 232, 737
PsuI RGATCY 1 cut(s) 623
PsyI GACNNNGTC 2 cut(s) 779, 827
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 561, 978
SatI GCNGC 6 cut(s) 519, 717, 797, 818, 957, 960
Sau3AI GATC 5 cut(s) 316, 337, 550, 623, 901
Sau96I GGNCC 2 cut(s) 232, 737
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 4 cut(s) 82, 392, 735, 840
SduI GDGCHC 1 cut(s) 80
SetI ASST 8 cut(s) 106, 429, 637, 718, 758, 810, 869, 885
SfaNI GCATC 1 cut(s) 337
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 2 cut(s) 232, 737
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 2 cut(s) 479, 696
SmoI CTYRAG 2 cut(s) 479, 696
Sse9I AATT 5 cut(s) 352, 429, 495, 662, 686
SsiI CCGC 3 cut(s) 740, 797, 959
SspMI CTAG 1 cut(s) 713
StyD4I CCNGG 4 cut(s) 80, 390, 733, 838
StyI CCWWGG 2 cut(s) 204, 235
TaaI ACNGT 3 cut(s) 169, 446, 876
TaiI ACGT 1 cut(s) 758
TaqI TCGA 3 cut(s) 315, 336, 707
TasI AATT 5 cut(s) 352, 429, 495, 662, 686
TauI GCSGC 2 cut(s) 799, 962
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 2 cut(s) 561, 978
Tru9I TTAA 2 cut(s) 561, 978
TscAI CASTG 3 cut(s) 121, 451, 553
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 4 cut(s) 518, 716, 817, 956
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 1 cut(s) 435
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 3 cut(s) 121, 451, 553
Tth111I GACNNNGTC 2 cut(s) 779, 827
VpaK11BI GGWCC 2 cut(s) 232, 737
XapI RAATTY 3 cut(s) 352, 495, 662
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.