RLG00000026885

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012379
Physical Location & Seq
Reverse (-)
6762267 .. 6766005
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026885

Sequence Viewer

Length: 852 bp
ATGTTGGAAAAGATTACCAAAATGGCAGAGGAGGTGAGTTTGAAGAGTACTACTACTACTCCTATTCACTTTGTGCTGGTGCATGGTATCAGTGGAGGGGGTTGGTGCTGGTACAAAATTCGGTGTCTCATGGAGAATTCCGGCTACAAAGTCTCCACAGTTGATCTGAAGAGCGCCGGAATCGATCCCGCAAACGCGGATTCGGTCCTCTCTTTCGACGACTACAACAAACCACTCCTCAACTTCCTCTCTTCTCTCCCCGAACACGAACAGGTAGTTTTGGTGGGGCACAGCGCCGGAGGGTTGAGCGTGACGCTGGCGACGCTGAAGTTTCCGAAGAAGATCCGGCTGGCGGTTTATGTGGCGGCGACGATGCTCAAGCATGGGTTCACTAGTGAGGAAGATGTCAAAGATGGAGTGCCTAATTTATCCGAATTTGGTGACGTGTACGAATTAGGATTTGAATTAGGAGCCGACAAACCACCAACAAGCGGAATTGTGAAGAAAGAAGTCCAGCGCAAAATCATATATCAAATGAGTCCTCAAGAGGATTCGACACTAGCTGCCATGCTTTTGAGACCAGGACCGCTCCTTGCTATAACGACAGCTCAGTTTAAGGAAGAAGACGATGACCATGACATTGATGCGCTTGAGAAAGTGCCACGTGTCTACGTGAAGACGTTGCAAGATCATGTGGTGAAACCGGCGCAGCAAGATTCGATGGTCAAGAGGTGGCCGCCGGCTGAGGTCTATGTATTGGATAGTGATCACAGTCCCTTCTTCTCCCAGCCGTTTCTGCTCTTTGGATTCCTTGTAAAGGCTGCCGAGGCTTCTCACTGGGAGATTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

284

Amino Acids

31.38

Weight (kDa)

5.38

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 23 - 262 1.2e-17 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 24 - 264 1.4e-17 Alpha/beta hydrolase family
Hydrolase_4 PF12146 25 - 245 2.1e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012379)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10870
fragaria_vesca FvH4_7g26180
malus_domestica MD01G1170100.v1.1 MD07G1237000.v1.1 MD11G1052400.v1.1
prunus_persica Prupe.2G266100_v2.0.a1
pyrus_communis pycom01g18280 pycom07g21330
rosa_chinensis RchiOBHm_Chr1g0372631
rosa_laevigata RLG00000026885
rosa_multiflora Rmu_sc0008757.1_g000010 Rmu_ssc0000419.1_g000007
rosa_roxburghii Rroxscaffold_4G00284810
rosa_rugosa Rorug01G0373400
rosa_samantha Rh1AG381900 Rh1BG345300 Rh1CG358700 Rh1DG376500
rosa_wichuraiana Rw1G033590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 669
AccII CGCG 1 cut(s) 197
AciI CCGC 7 cut(s) 189, 197, 353, 365, 492, 587, 737
AclWI GGATC 2 cut(s) 179, 337
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 3 cut(s) 117, 136, 434
AcuI CTGAAG 2 cut(s) 188, 347
AcvI CACGTG 1 cut(s) 665
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 3 cut(s) 49, 113, 449
AfiI CCNNNNNNNGG 3 cut(s) 352, 491, 817
AflIII ACRYGT 2 cut(s) 444, 664
AgsI TTSAA 2 cut(s) 43, 464
AhlI ACTAGT 1 cut(s) 392
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 580
AluBI AGCT 2 cut(s) 563, 608
AluI AGCT 2 cut(s) 563, 608
Alw26I GTCTC 3 cut(s) 131, 157, 571
AlwI GGATC 2 cut(s) 179, 337
AoxI GGCC 1 cut(s) 734
ApeKI GCWGC 3 cut(s) 563, 709, 821
ApoI RAATTY 3 cut(s) 117, 136, 434
AspLEI GCGC 5 cut(s) 176, 296, 519, 649, 709
AspS9I GGNCC 2 cut(s) 205, 584
AsuHPI GGTGA 3 cut(s) 46, 452, 709
AvaII GGWCC 2 cut(s) 205, 584
BaeGI GKGCMC 1 cut(s) 291
BbrPI CACGTG 1 cut(s) 665
BbsI GAAGAC 2 cut(s) 630, 683
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 3 cut(s) 550, 721, 808
BccI CCATC 2 cut(s) 407, 715
BceAI ACGGC 1 cut(s) 775
BciT130I CCWGG 1 cut(s) 582
BclI TGATCA 1 cut(s) 766
BcoDI GTCTC 3 cut(s) 131, 157, 571
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 2 cut(s) 393, 560
BfoI RGCGCY 2 cut(s) 177, 297
BisI GCNGC 5 cut(s) 366, 564, 710, 737, 822
BlsI GCNGC 5 cut(s) 367, 565, 711, 738, 823
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 2 cut(s) 205, 584
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 205, 584
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 582
BmrI ACTGGG 1 cut(s) 847
BmsI GCATC 2 cut(s) 363, 634
BmuI ACTGGG 1 cut(s) 847
BpiI GAAGAC 2 cut(s) 630, 683
Bpu10I CCTNAGC 1 cut(s) 744
BpuEI CTTGAG 3 cut(s) 362, 528, 671
Bsa29I ATCGAT 1 cut(s) 183
BsaAI YACGTR 2 cut(s) 665, 673
BsaBI GATNNNNATC 1 cut(s) 765
BsaI GGTCTC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 825
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 491, 817
Bse118I RCCGGY 2 cut(s) 703, 739
Bse1I ACTGG 1 cut(s) 842
Bse8I GATNNNNATC 1 cut(s) 765
BseBI CCWGG 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 825
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 3 cut(s) 352, 491, 817
BseMII CTCAG 2 cut(s) 623, 735
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 2 cut(s) 44, 227
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 3 cut(s) 550, 721, 808
BseYI CCCAGC 1 cut(s) 786
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 1 cut(s) 736
BshVI ATCGAT 1 cut(s) 183
BsiSI CCGG 6 cut(s) 141, 177, 297, 346, 704, 740
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 3 cut(s) 352, 491, 817
BsmAI GTCTC 3 cut(s) 131, 157, 571
BsmFI GGGAC 1 cut(s) 759
BsnI GGCC 1 cut(s) 736
Bso31I GGTCTC 1 cut(s) 571
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 5 cut(s) 163, 184, 342, 688, 766
BspACI CCGC 7 cut(s) 189, 197, 353, 365, 492, 587, 737
BspANI GGCC 1 cut(s) 736
BspCNI CTCAG 2 cut(s) 622, 736
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 472
BspPI GGATC 2 cut(s) 179, 337
BspQI GCTCTTC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 571
BsrBI CCGCTC 1 cut(s) 589
BsrFI RCCGGY 2 cut(s) 703, 739
BsrI ACTGG 1 cut(s) 842
BssAI RCCGGY 2 cut(s) 703, 739
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 5 cut(s) 163, 184, 342, 688, 766
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 160, 773
Bst6I CTCTTC 3 cut(s) 38, 164, 256
BstBAI YACGTR 2 cut(s) 665, 673
BstC8I GCNNGC 3 cut(s) 318, 351, 741
BstDEI CTNAG 2 cut(s) 609, 744
BstENI CCTNNNNNAGG 1 cut(s) 815
BstFNI CGCG 1 cut(s) 197
BstH2I RGCGCY 2 cut(s) 177, 297
BstHHI GCGC 5 cut(s) 176, 296, 519, 649, 709
BstKTI GATC 5 cut(s) 166, 187, 345, 691, 769
BstMAI GTCTC 3 cut(s) 131, 157, 571
BstMBI GATC 5 cut(s) 163, 184, 342, 688, 766
BstMWI GCNNNNNNNGC 3 cut(s) 322, 796, 827
BstNI CCWGG 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 580
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 3 cut(s) 550, 721, 808
BstV2I GAAGAC 2 cut(s) 630, 683
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 1 cut(s) 736
BsuTUI ATCGAT 1 cut(s) 183
BtrI CACGTC 1 cut(s) 445
BtsIMutI CAGTG 2 cut(s) 97, 835
Cac8I GCNNGC 3 cut(s) 318, 351, 741
CfoI GCGC 5 cut(s) 176, 296, 519, 649, 709
Cfr10I RCCGGY 2 cut(s) 703, 739
Cfr13I GGNCC 2 cut(s) 205, 584
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 2 cut(s) 322, 331
Csp6I GTAC 3 cut(s) 48, 112, 448
CviAII CATG 6 cut(s) 83, 130, 383, 568, 635, 692
CviQI GTAC 3 cut(s) 48, 112, 448
DdeI CTNAG 2 cut(s) 609, 744
DpnI GATC 5 cut(s) 165, 186, 344, 690, 768
DpnII GATC 5 cut(s) 163, 184, 342, 688, 766
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 3 cut(s) 38, 164, 256
EarI CTCTTC 3 cut(s) 38, 164, 256
Eco31I GGTCTC 1 cut(s) 571
Eco47I GGWCC 2 cut(s) 205, 584
Eco57I CTGAAG 2 cut(s) 188, 347
Eco72I CACGTG 1 cut(s) 665
EcoNI CCTNNNNNAGG 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 580
FaeI CATG 6 cut(s) 86, 133, 386, 571, 638, 695
FaqI GGGAC 1 cut(s) 759
FatI CATG 6 cut(s) 82, 129, 382, 567, 634, 691
FauI CCCGC 1 cut(s) 196
FbaI TGATCA 1 cut(s) 766
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 5 cut(s) 366, 564, 710, 737, 822
Fsp4HI GCNGC 5 cut(s) 366, 564, 710, 737, 822
FspBI CTAG 2 cut(s) 393, 560
GlaI GCGC 5 cut(s) 175, 295, 518, 648, 708
GluI GCNGC 5 cut(s) 366, 564, 710, 737, 822
GsaI CCCAGC 1 cut(s) 790
HaeII RGCGCY 2 cut(s) 177, 297
HaeIII GGCC 1 cut(s) 736
HapII CCGG 6 cut(s) 141, 177, 297, 346, 704, 740
HgaI GACGC 2 cut(s) 322, 331
HhaI GCGC 5 cut(s) 176, 296, 519, 649, 709
Hin1II CATG 6 cut(s) 86, 133, 386, 571, 638, 695
Hin6I GCGC 5 cut(s) 174, 294, 517, 647, 707
HinP1I GCGC 5 cut(s) 174, 294, 517, 647, 707
HinfI GANTC 6 cut(s) 180, 200, 538, 551, 716, 807
HpaII CCGG 6 cut(s) 141, 177, 297, 346, 704, 740
HphI GGTGA 3 cut(s) 46, 452, 709
Hpy166II GTNNAC 3 cut(s) 390, 448, 670
Hpy188I TCNGA 3 cut(s) 168, 336, 433
Hpy188III TCNNGA 2 cut(s) 545, 727
Hpy8I GTNNAC 3 cut(s) 390, 448, 670
Hpy99I CGWCG 3 cut(s) 221, 325, 373
HpyAV CCTTC 1 cut(s) 787
HpyCH4III ACNGT 2 cut(s) 160, 773
HpyCH4IV ACGT 4 cut(s) 444, 664, 672, 680
HpyCH4V TGCA 2 cut(s) 82, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 796, 827
HpyF3I CTNAG 2 cut(s) 609, 744
HpySE526I ACGT 4 cut(s) 444, 664, 672, 680
Hsp92II CATG 6 cut(s) 86, 133, 386, 571, 638, 695
HspAI GCGC 5 cut(s) 174, 294, 517, 647, 707
KroI GCCGGC 1 cut(s) 739
KroNI GCCGGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 5 cut(s) 163, 184, 342, 688, 766
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 470, 594
Lsp1109I GCAGC 3 cut(s) 550, 721, 808
LweI GCATC 2 cut(s) 363, 634
MaeI CTAG 2 cut(s) 393, 560
MaeII ACGT 4 cut(s) 444, 664, 672, 680
MaeIII GTNAC 2 cut(s) 310, 440
MalI GATC 5 cut(s) 165, 186, 344, 690, 768
MbiI CCGCTC 1 cut(s) 589
MboI GATC 5 cut(s) 163, 184, 342, 688, 766
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 7 cut(s) 117, 136, 424, 434, 452, 464, 495
MlyI GAGTC 1 cut(s) 547
MroNI GCCGGC 1 cut(s) 739
MseI TTAA 1 cut(s) 615
MspI CCGG 6 cut(s) 141, 177, 297, 346, 704, 740
MspR9I CCNGG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 582
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 322, 796, 827
NaeI GCCGGC 1 cut(s) 741
NdeII GATC 5 cut(s) 163, 184, 342, 688, 766
NgoMIV GCCGGC 1 cut(s) 739
NlaIII CATG 6 cut(s) 86, 133, 386, 571, 638, 695
NlaIV GGNNCC 1 cut(s) 472
NmeAIII GCCGAG 1 cut(s) 850
NmuCI GTSAC 2 cut(s) 310, 440
PciSI GCTCTTC 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 741
PfeI GAWTC 5 cut(s) 180, 200, 551, 716, 807
PkrI GCNGC 5 cut(s) 367, 565, 711, 738, 823
PleI GAGTC 1 cut(s) 546
PmaCI CACGTG 1 cut(s) 665
PmlI CACGTG 1 cut(s) 665
PpsI GAGTC 1 cut(s) 546
Ppu21I YACGTR 2 cut(s) 665, 673
Psp6I CCWGG 1 cut(s) 580
PspCI CACGTG 1 cut(s) 665
PspFI CCCAGC 1 cut(s) 786
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 2 cut(s) 205, 584
PsuI RGATCY 1 cut(s) 342
RsaI GTAC 3 cut(s) 49, 113, 449
RsaNI GTAC 3 cut(s) 48, 112, 448
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 5 cut(s) 366, 564, 710, 737, 822
Sau3AI GATC 5 cut(s) 163, 184, 342, 688, 766
Sau96I GGNCC 2 cut(s) 205, 584
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 2 cut(s) 363, 634
SinI GGWCC 2 cut(s) 205, 584
SmlI CTYRAG 3 cut(s) 377, 543, 650
SmoI CTYRAG 3 cut(s) 377, 543, 650
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 7 cut(s) 117, 136, 424, 434, 452, 464, 495
SsiI CCGC 7 cut(s) 189, 197, 353, 365, 492, 587, 737
SspMI CTAG 2 cut(s) 393, 560
StyD4I CCNGG 1 cut(s) 580
TaaI ACNGT 2 cut(s) 160, 773
TaiI ACGT 4 cut(s) 447, 667, 675, 683
TaqI TCGA 4 cut(s) 183, 216, 554, 719
TaqII GACCGA 1 cut(s) 193
TasI AATT 7 cut(s) 117, 136, 424, 434, 452, 464, 495
TatI WGTACW 1 cut(s) 47
TauI GCSGC 2 cut(s) 368, 739
TfiI GAWTC 5 cut(s) 180, 200, 551, 716, 807
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 2 cut(s) 97, 842
TseFI GTSAC 2 cut(s) 310, 440
TseI GCWGC 3 cut(s) 563, 709, 821
Tsp45I GTSAC 2 cut(s) 310, 440
TspRI CASTG 2 cut(s) 97, 842
VpaK11BI GGWCC 2 cut(s) 205, 584
XagI CCTNNNNNAGG 1 cut(s) 815
XapI RAATTY 3 cut(s) 117, 136, 434
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 2 cut(s) 393, 560
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.