FvH4_7g26970

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006869
Physical Location & Seq
Reverse (-)
20242882 .. 20247782
4901 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26970.t1

Sequence Viewer

Length: 1077 bp
ATGGAGTTCACCGAGGCTTTCAAGCAGACCGGTCCTTGCTGCTTCTCCCCCAATGCTCGCTACATTGCCGTCGCCGTCGATTACCGCCTCGTGATTCGCGACACTCTCTCCTTCAAGGTTGTGCAGTTATTCTCATGCTTGGACAAGATAAGCTACATTGAATGGGCTCTTGATTCTGAGTATATTCTTTGTGGTCTATATAAAAGACCAATGATACAGGCATGGTCGCTGGCCCAACCTGAATGGACATGCAAGATAGATGAAGGTCCTGCCGGCATTGCTTATGCTAGGTGGAGCCCAGATAGCCGTCATATACTTACTACATCAGACTTCCAACTGCGTTTAACAGTTTGGTCTCTCTTAAACACAGCATGTGTACATGTCCAATGGCCTAAGCATCTTTCAAAGGGGGTTTCATTCACCAAAGATGGGAAATTCGCTGCAATTTGCACAAGGCGCGATTGCAAGGATTATATAAATCTTCTGTCTTGTCAAACATGGGAAATAATGGGTGTATATGCTGTGGACACAGTGGACTTGGCTGATGCTGAATGGTCACCTGATGACAGTGCAATAGTGATATGGGATTCTTCTCTTGAATATAAGGTTCTAATATACTCCCCAGATGGAAGGTGCCTATTTAAGTATCAAGCATATGAAAGTGGATTGGGGGTAAAAAGTGTTTCATGGTCTCCCTGTGGCCAGTTTCTAGCAGTGGGTAGTTATGATCAGATGTTGCGGGTTTTGAATCACCTGACATGGAAGACTTTTGCAGAATTTATGCATCTATCTACTGTTCGTGCTCCGTGTTGTGCTGCTGTCTTCAAGGAAGTGGATGAGCCACTAGTACTCGATATGTCAGAGTTATGCTTGACTGATGATTTTGAACGTGGAAATAATGAAGATGCTTCTGAAGGACACATCAGAGTTAGGTATGAGGTTACAGAAGTGCCCATCACTTTGCCTTTTCAGAAGCCTCCTGGTGACAAACCTAACCCAAAACAAGGAATTGGTCTACATGCCATTGAATTATCAATATGCATATGTGAGCGGGTTGGTTGTGATAGATGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

359

Amino Acids

40.55

Weight (kDa)

5.19

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 13 - 192 9.3e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 633
AccBSI CCGCTC 1 cut(s) 1051
AccI GTMKAC 1 cut(s) 1015
AccII CGCG 2 cut(s) 99, 459
AciI CCGC 3 cut(s) 85, 739, 1051
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 434, 776
AcuI CTGAAG 1 cut(s) 933
AfaI GTAC 2 cut(s) 378, 849
AfiI CCNNNNNNNGG 2 cut(s) 429, 1004
AflIII ACRYGT 1 cut(s) 379
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 9 cut(s) 22, 115, 161, 405, 599, 748, 826, 887, 1028
AhlI ACTAGT 1 cut(s) 844
AjnI CCWGG 1 cut(s) 979
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 2 cut(s) 360, 696
AoxI GGCC 3 cut(s) 231, 389, 700
ApeKI GCWGC 3 cut(s) 39, 440, 815
ApoI RAATTY 2 cut(s) 434, 776
AsiGI ACCGGT 1 cut(s) 29
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 32, 232, 266
AsuHPI GGTGA 4 cut(s) 412, 549, 743, 995
AvaII GGWCC 2 cut(s) 32, 266
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 1 cut(s) 702
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 2 cut(s) 169, 299
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 2 cut(s) 770, 814
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 3 cut(s) 26, 427, 802
BccI CCATC 3 cut(s) 422, 620, 962
BceAI ACGGC 3 cut(s) 53, 59, 291
BciT130I CCWGG 1 cut(s) 981
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 2 cut(s) 360, 696
BcuI ACTAGT 1 cut(s) 844
BfaI CTAG 3 cut(s) 288, 710, 845
BisI GCNGC 3 cut(s) 40, 441, 816
BlsI GCNGC 3 cut(s) 41, 442, 817
BmcAI AGTACT 1 cut(s) 849
Bme1390I CCNGG 1 cut(s) 981
Bme18I GGWCC 2 cut(s) 32, 266
BmgT120I GGNCC 3 cut(s) 32, 232, 266
BmiI GGNNCC 2 cut(s) 296, 635
BmrFI CCNGG 1 cut(s) 981
BmsI GCATC 5 cut(s) 406, 535, 793, 895, 1058
BpiI GAAGAC 2 cut(s) 770, 814
Bpu10I CCTNAGC 1 cut(s) 393
BsaI GGTCTC 2 cut(s) 360, 696
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 1004
Bse118I RCCGGY 2 cut(s) 29, 272
Bse1I ACTGG 1 cut(s) 703
Bse3DI GCAATG 2 cut(s) 63, 276
BseBI CCWGG 1 cut(s) 981
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 841
BseLI CCNNNNNNNGG 2 cut(s) 429, 1004
BseMI GCAATG 2 cut(s) 63, 276
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 703
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 3 cut(s) 26, 427, 802
BsgI GTGCAG 1 cut(s) 143
Bsh1236I CGCG 2 cut(s) 99, 459
BshFI GGCC 3 cut(s) 233, 391, 702
BshNI GGYRCC 1 cut(s) 633
BshTI ACCGGT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 2 cut(s) 30, 273
BslI CCNNNNNNNGG 2 cut(s) 429, 1004
BsmAI GTCTC 2 cut(s) 360, 696
BsnI GGCC 3 cut(s) 233, 391, 702
Bso31I GGTCTC 2 cut(s) 360, 696
Bsp1286I GDGCHC 4 cut(s) 169, 299, 805, 954
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 727
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 3 cut(s) 85, 739, 1051
BspANI GGCC 3 cut(s) 233, 391, 702
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 2 cut(s) 99, 459
BspLI GGNNCC 2 cut(s) 296, 635
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 2 cut(s) 360, 696
BsrBI CCGCTC 1 cut(s) 1051
BsrDI GCAATG 2 cut(s) 63, 276
BsrFI RCCGGY 2 cut(s) 29, 272
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 1 cut(s) 703
BssAI RCCGGY 2 cut(s) 29, 272
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 727
BssSI CACGAG 1 cut(s) 89
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 981
Bst4CI ACNGT 4 cut(s) 349, 532, 569, 796
BstAUI TGTACA 1 cut(s) 376
BstC8I GCNNGC 3 cut(s) 58, 231, 274
BstDEI CTNAG 2 cut(s) 177, 393
BstEII GGTNACC 1 cut(s) 555
BstF5I GGATG 1 cut(s) 841
BstFNI CGCG 2 cut(s) 99, 459
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 730
BstMAI GTCTC 2 cut(s) 360, 696
BstMBI GATC 1 cut(s) 727
BstMWI GCNNNNNNNGC 3 cut(s) 278, 303, 456
BstNI CCWGG 1 cut(s) 981
BstNSI RCATGY 4 cut(s) 252, 375, 383, 1022
BstPI GGTNACC 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 2 cut(s) 99, 459
BstV1I GCAGC 3 cut(s) 26, 427, 802
BstV2I GAAGAC 2 cut(s) 770, 814
BsuRI GGCC 3 cut(s) 233, 391, 702
BtsCI GGATG 1 cut(s) 841
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 3 cut(s) 537, 574, 720
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 58, 231, 274
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 2 cut(s) 29, 272
Cfr13I GGNCC 3 cut(s) 32, 232, 266
Csp6I GTAC 2 cut(s) 377, 848
CspAI ACCGGT 1 cut(s) 29
CviAII CATG 9 cut(s) 135, 222, 249, 372, 380, 498, 687, 759, 1019
CviQI GTAC 2 cut(s) 377, 848
DdeI CTNAG 2 cut(s) 177, 393
DpnI GATC 1 cut(s) 729
DpnII GATC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 700
Eco24I GRGCYC 2 cut(s) 169, 299
Eco31I GGTCTC 2 cut(s) 360, 696
Eco47I GGWCC 2 cut(s) 32, 266
Eco57I CTGAAG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 979
EcoT22I ATGCAT 2 cut(s) 786, 1043
EcoT38I GRGCYC 2 cut(s) 169, 299
FaeI CATG 9 cut(s) 138, 225, 252, 375, 383, 501, 690, 762, 1022
FatI CATG 9 cut(s) 134, 221, 248, 371, 379, 497, 686, 758, 1018
FauI CCCGC 2 cut(s) 732, 1044
FauNDI CATATG 2 cut(s) 655, 1043
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 3 cut(s) 40, 441, 816
FokI GGATG 1 cut(s) 848
FriOI GRGCYC 2 cut(s) 169, 299
Fsp4HI GCNGC 3 cut(s) 40, 441, 816
FspBI CTAG 3 cut(s) 288, 710, 845
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 40, 441, 816
HaeIII GGCC 3 cut(s) 233, 391, 702
HapII CCGG 2 cut(s) 30, 273
HhaI GCGC 1 cut(s) 459
Hin1II CATG 9 cut(s) 138, 225, 252, 375, 383, 501, 690, 762, 1022
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 4 cut(s) 94, 173, 587, 748
HpaII CCGG 2 cut(s) 30, 273
HphI GGTGA 4 cut(s) 412, 549, 743, 995
Hpy166II GTNNAC 5 cut(s) 9, 377, 526, 535, 1016
Hpy188I TCNGA 7 cut(s) 178, 328, 732, 862, 913, 926, 972
Hpy188III TCNNGA 5 cut(s) 91, 98, 170, 596, 1074
Hpy8I GTNNAC 5 cut(s) 9, 377, 526, 535, 1016
Hpy99I CGWCG 2 cut(s) 74, 80
HpyAV CCTTC 4 cut(s) 121, 257, 624, 908
HpyCH4III ACNGT 4 cut(s) 349, 532, 569, 796
HpyCH4IV ACGT 1 cut(s) 889
HpyCH4V TGCA 9 cut(s) 124, 252, 443, 450, 465, 572, 773, 784, 1041
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 303, 456
HpyF3I CTNAG 2 cut(s) 177, 393
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 9 cut(s) 138, 225, 252, 375, 383, 501, 690, 762, 1022
HspAI GCGC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 1 cut(s) 727
LmnI GCTCC 2 cut(s) 294, 808
Lsp1109I GCAGC 3 cut(s) 26, 427, 802
LweI GCATC 5 cut(s) 406, 535, 793, 895, 1058
MaeI CTAG 3 cut(s) 288, 710, 845
MaeII ACGT 1 cut(s) 889
MaeIII GTNAC 3 cut(s) 555, 940, 983
MalI GATC 1 cut(s) 729
MbiI CCGCTC 1 cut(s) 1051
MboI GATC 1 cut(s) 727
MboII GAAGA 5 cut(s) 473, 582, 775, 814, 914
MhlI GDGCHC 4 cut(s) 169, 299, 805, 954
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 5 cut(s) 434, 444, 776, 1008, 1028
MluNI TGGCCA 1 cut(s) 702
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 4 cut(s) 7, 98, 931, 987
Mox20I TGGCCA 1 cut(s) 702
Mph1103I ATGCAT 2 cut(s) 786, 1043
MroNI GCCGGC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 702
MseI TTAA 3 cut(s) 344, 362, 642
Msp20I TGGCCA 1 cut(s) 702
MspI CCGG 2 cut(s) 30, 273
MspR9I CCNGG 1 cut(s) 981
MvaI CCWGG 1 cut(s) 981
MvnI CGCG 2 cut(s) 99, 459
MwoI GCNNNNNNNGC 3 cut(s) 278, 303, 456
NaeI GCCGGC 1 cut(s) 274
NdeI CATATG 2 cut(s) 655, 1043
NdeII GATC 1 cut(s) 727
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 9 cut(s) 138, 225, 252, 375, 383, 501, 690, 762, 1022
NlaIV GGNNCC 2 cut(s) 296, 635
NmuCI GTSAC 2 cut(s) 555, 983
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 2 cut(s) 786, 1043
NspI RCATGY 4 cut(s) 252, 375, 383, 1022
PciI ACATGT 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 4 cut(s) 94, 173, 587, 748
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 3 cut(s) 41, 442, 817
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 979
PspEI GGTNACC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 296, 635
PspPI GGNCC 3 cut(s) 32, 232, 266
PspPPI RGGWCCY 1 cut(s) 266
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 2 cut(s) 378, 849
RsaNI GTAC 2 cut(s) 377, 848
SaqAI TTAA 3 cut(s) 344, 362, 642
SatI GCNGC 3 cut(s) 40, 441, 816
Sau3AI GATC 1 cut(s) 727
Sau96I GGNCC 3 cut(s) 32, 232, 266
ScaI AGTACT 1 cut(s) 849
ScrFI CCNGG 1 cut(s) 981
SduI GDGCHC 4 cut(s) 169, 299, 805, 954
SfaNI GCATC 5 cut(s) 406, 535, 793, 895, 1058
SinI GGWCC 2 cut(s) 32, 266
SpeI ACTAGT 1 cut(s) 844
Sse9I AATT 5 cut(s) 434, 444, 776, 1008, 1028
SsiI CCGC 3 cut(s) 85, 739, 1051
SspMI CTAG 3 cut(s) 288, 710, 845
StyD4I CCNGG 1 cut(s) 979
TaaI ACNGT 4 cut(s) 349, 532, 569, 796
TaiI ACGT 1 cut(s) 892
TaqI TCGA 2 cut(s) 78, 852
TasI AATT 5 cut(s) 434, 444, 776, 1008, 1028
TatI WGTACW 2 cut(s) 376, 847
TfiI GAWTC 4 cut(s) 94, 173, 587, 748
Tru1I TTAA 3 cut(s) 344, 362, 642
Tru9I TTAA 3 cut(s) 344, 362, 642
TscAI CASTG 3 cut(s) 537, 574, 720
TseFI GTSAC 2 cut(s) 555, 983
TseI GCWGC 3 cut(s) 39, 440, 815
Tsp45I GTSAC 2 cut(s) 555, 983
TspDTI ATGAA 5 cut(s) 276, 405, 672, 675, 915
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 3 cut(s) 537, 574, 720
VpaK11BI GGWCC 2 cut(s) 32, 266
XapI RAATTY 2 cut(s) 434, 776
XceI RCATGY 4 cut(s) 252, 375, 383, 1022
XmiI GTMKAC 1 cut(s) 1015
XspI CTAG 3 cut(s) 288, 710, 845
ZrmI AGTACT 1 cut(s) 849
Zsp2I ATGCAT 2 cut(s) 786, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.