pycom01g18800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006869
Physical Location & Seq
Reverse (-)
17650151 .. 17653796
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18800.1

Sequence Viewer

Length: 1383 bp
ATGGAGTTCACAGAGGCTTACAAGCAGACCGGTCCTTGTTGCTTCTCACCTAATGCTCGCTACATTTCCGTCGCCGTCGACTACCGCCTCGTCATTCGCGACACTCTTTCCTTTAAGGTTGTGCAGTTGTTCTCATGTTTGGATAAGATAAGCTACATCGAATGGGCGCTTGATTCTGAATACATTCTTTGTGGTCTATATAAAAGACCAATGATACAAGCATGGTCGCTGGCCCAACCTGAATGGACATGTAAGATAGATGAAGGTCCTGCCGGTATTGCCTATGCTAGGTGGAGCCCAGATAGCCGTCACATACTTACCACATCAGACTTCCAACTGCGGTTAACAGTTTGGTCACTGTTAAACACAGCATGTGTACATGTGCAATGGCCAAAGCATCCTTCCAAGGGAGTTTCGTTCACCAAAGATGGGCAATTTGCTGCAATTTGCACAAGGCGTGATTGCAAGGACTATATTAATCTTCTGTCCTGTCATACATGGGAAATAATGGGTGTATTTGCTGTGGACACTTTGGACTTGGCTGATACTGAATGGTCACCAGATGACAGTGCAATAGTGATATGGGATTCGCCTCTCGAATATAAGGTTCTGATATACTCCCCAGATGGGAGGTGTCTGTATAAGTATCAAGCATACGAAAGTGCTTTGGGTGTAAAAAGTGTTTCATGGTCTCCGTGTGGCCAGTTTCTAGCAGTGGGTAGTTATGACCAGATGTTGCGGGTTTTAAATCACTTGACTTGGAAGACTTTTGCTGAATTTATGCATCTATCTACTGTCCGTGCTCCTTGTTGTGCGGCTGTGTTTAAGGAAGTAGATGAGCCACTGCTACTTGATATGTCCGAGCTAAGTCTAAATGATGATTTTGCAGAAGGCAATAATGATGCTTCGGAAGGACAAGTCAGAGTTAGATACGAGGTTACAGAAGTGCCCATCAGTTTGCCTTTCCAGAAGCCTCCTGCAGACAAACCTAACCCCAAACAAGGAATTGGTCTAATGTCATGGAGCAACGATAGCCAATATATTTGTACTCGCAACGATAGCATGCCAAGCATTCTCTGGATATGGGACATACGCCATCTTGAACTCGCTGCTATCTTGGTGCAGAAAGATCCTATTCGTGCAGCAGTTTGGGATCCTACGTGCACTCGTCTTGTTGTTTGCACGGGAAGTTCTCATCTGTACATGTGGACTCCTGGTGGTGCCTACTGTGTGAGCATCCCTCCGCAACCACAGTTTAGCATAGTTGATCTGAAATGGAATTCGGATGGAAGTTGTCTTCTCCTGAAGGATAAAGAGTTGTTTTGCTGCGCTGCTGTCGCCGTTCTTCCTGAAGAATCGAGTGAATATAGCTCGGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

461

Amino Acids

52.01

Weight (kDa)

4.9

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 13 - 192 1e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1220
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 99
AciI CCGC 5 cut(s) 85, 340, 739, 815, 1244
AclWI GGATC 3 cut(s) 1124, 1148, 1161
AcoI YGGCCR 2 cut(s) 389, 700
AcsI RAATTY 2 cut(s) 776, 1279
AcuI CTGAAG 2 cut(s) 1325, 1371
AfaI GTAC 3 cut(s) 378, 1048, 1202
AfiI CCNNNNNNNGG 5 cut(s) 288, 407, 429, 627, 1001
AflIII ACRYGT 3 cut(s) 248, 379, 1203
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 1 cut(s) 1103
AjnI CCWGG 1 cut(s) 1213
AjuI GAANNNNNNNTTGG 2 cut(s) 385, 417
AluBI AGCT 3 cut(s) 153, 865, 1371
AluI AGCT 3 cut(s) 153, 865, 1371
Alw21I GWGCWC 2 cut(s) 805, 1166
Alw26I GTCTC 1 cut(s) 696
Alw44I GTGCAC 1 cut(s) 1162
AlwI GGATC 3 cut(s) 1124, 1148, 1161
AoxI GGCC 3 cut(s) 231, 389, 700
ApaLI GTGCAC 1 cut(s) 1162
ApeKI GCWGC 5 cut(s) 440, 1109, 1142, 1326, 1331
ApoI RAATTY 2 cut(s) 776, 1279
AseI ATTAAT 1 cut(s) 477
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 2 cut(s) 169, 1331
AspS9I GGNCC 3 cut(s) 32, 232, 266
AsuHPI GGTGA 3 cut(s) 39, 412, 549
AvaII GGWCC 2 cut(s) 32, 266
BaeGI GKGCMC 2 cut(s) 951, 1166
BalI TGGCCA 2 cut(s) 391, 702
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 1 cut(s) 1220
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 2 cut(s) 770, 1289
Bbv12I GWGCWC 2 cut(s) 805, 1166
BbvI GCAGC 5 cut(s) 427, 1096, 1154, 1313, 1318
BccI CCATC 5 cut(s) 422, 620, 959, 1104, 1280
BceAI ACGGC 3 cut(s) 59, 291, 1325
BciT130I CCWGG 1 cut(s) 1215
BcoDI GTCTC 1 cut(s) 696
BfaI CTAG 2 cut(s) 288, 710
BfmI CTRYAG 1 cut(s) 978
BfoI RGCGCY 1 cut(s) 170
BisI GCNGC 6 cut(s) 441, 816, 1110, 1143, 1327, 1332
BlsI GCNGC 6 cut(s) 442, 817, 1111, 1144, 1328, 1333
Bme1390I CCNGG 1 cut(s) 1215
Bme18I GGWCC 2 cut(s) 32, 266
BmgT120I GGNCC 3 cut(s) 32, 232, 266
BmiI GGNNCC 3 cut(s) 296, 1155, 1222
BmrFI CCNGG 1 cut(s) 1215
BmsI GCATC 4 cut(s) 406, 793, 892, 1245
BpiI GAAGAC 2 cut(s) 770, 1289
BplI GAGNNNNNCTC 2 cut(s) 1225, 1257
BsaAI YACGTR 1 cut(s) 1161
BsaI GGTCTC 1 cut(s) 696
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 5 cut(s) 288, 407, 429, 627, 1001
Bse118I RCCGGY 2 cut(s) 29, 272
Bse1I ACTGG 1 cut(s) 703
Bse3DI GCAATG 1 cut(s) 392
BseBI CCWGG 1 cut(s) 1215
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 4 cut(s) 397, 1236, 1291, 1381
BseLI CCNNNNNNNGG 5 cut(s) 288, 407, 429, 627, 1001
BseMI GCAATG 1 cut(s) 392
BseNI ACTGG 1 cut(s) 703
BseSI GKGCMC 2 cut(s) 951, 1166
BseXI GCAGC 5 cut(s) 427, 1096, 1154, 1313, 1318
BsgI GTGCAG 3 cut(s) 143, 1142, 1161
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 3 cut(s) 233, 391, 702
BshNI GGYRCC 1 cut(s) 1220
BshTI ACCGGT 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 805, 1166
BsiSI CCGG 2 cut(s) 30, 273
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 5 cut(s) 288, 407, 429, 627, 1001
BsmAI GTCTC 1 cut(s) 696
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 1 cut(s) 1071
BsnI GGCC 3 cut(s) 233, 391, 702
Bso31I GGTCTC 1 cut(s) 696
Bsp1286I GDGCHC 4 cut(s) 299, 805, 951, 1166
Bsp1407I TGTACA 2 cut(s) 376, 1200
Bsp143I GATC 3 cut(s) 1129, 1153, 1267
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 5 cut(s) 85, 340, 739, 815, 1244
BspANI GGCC 3 cut(s) 233, 391, 702
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 3 cut(s) 296, 1155, 1222
BspMAI CTGCAG 1 cut(s) 982
BspPI GGATC 3 cut(s) 1124, 1148, 1161
BspT107I GGYRCC 1 cut(s) 1220
BspTNI GGTCTC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 392
BsrFI RCCGGY 2 cut(s) 29, 272
BsrGI TGTACA 2 cut(s) 376, 1200
BsrI ACTGG 1 cut(s) 703
BssAI RCCGGY 2 cut(s) 29, 272
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 3 cut(s) 1129, 1153, 1267
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 1215
Bst4CI ACNGT 6 cut(s) 349, 360, 569, 796, 1229, 1254
BstAUI TGTACA 2 cut(s) 376, 1200
BstBAI YACGTR 1 cut(s) 1161
BstC8I GCNNGC 3 cut(s) 58, 231, 1064
BstDEI CTNAG 1 cut(s) 866
BstEII GGTNACC 1 cut(s) 555
BstENI CCTNNNNNAGG 1 cut(s) 286
BstF5I GGATG 4 cut(s) 397, 1236, 1291, 1381
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 169, 1331
BstKTI GATC 3 cut(s) 1132, 1156, 1270
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 3 cut(s) 1129, 1153, 1267
BstMWI GCNNNNNNNGC 6 cut(s) 278, 303, 1032, 1059, 1068, 1337
BstNI CCWGG 1 cut(s) 1215
BstNSI RCATGY 5 cut(s) 252, 375, 383, 1066, 1207
BstPI GGTNACC 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 1213
BstSFI CTRYAG 1 cut(s) 978
BstSLI GKGCMC 2 cut(s) 951, 1166
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 5 cut(s) 427, 1096, 1154, 1313, 1318
BstV2I GAAGAC 2 cut(s) 770, 1289
BstX2I RGATCY 2 cut(s) 1129, 1153
BstYI RGATCY 2 cut(s) 1129, 1153
BsuRI GGCC 3 cut(s) 233, 391, 702
BtsCI GGATG 4 cut(s) 397, 1236, 1291, 1381
BtsI GCAGTG 2 cut(s) 720, 842
BtsIMutI CAGTG 4 cut(s) 356, 574, 720, 842
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 3 cut(s) 58, 231, 1064
CfoI GCGC 2 cut(s) 169, 1331
Cfr10I RCCGGY 2 cut(s) 29, 272
Cfr13I GGNCC 3 cut(s) 32, 232, 266
Csp6I GTAC 3 cut(s) 377, 1047, 1201
CspAI ACCGGT 1 cut(s) 29
CviQI GTAC 3 cut(s) 377, 1047, 1201
DdeI CTNAG 1 cut(s) 866
DpnI GATC 3 cut(s) 1131, 1155, 1269
DpnII GATC 3 cut(s) 1129, 1153, 1267
DraI TTTAAA 1 cut(s) 747
EaeI YGGCCR 2 cut(s) 389, 700
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 2 cut(s) 32, 266
Eco57I CTGAAG 2 cut(s) 1325, 1371
Eco91I GGTNACC 1 cut(s) 555
EcoNI CCTNNNNNAGG 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 1279
EcoRII CCWGG 1 cut(s) 1213
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 1 cut(s) 786
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 405
FaqI GGGAC 1 cut(s) 1100
FauI CCCGC 1 cut(s) 732
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 6 cut(s) 441, 816, 1110, 1143, 1327, 1332
FokI GGATG 3 cut(s) 384, 1223, 1298
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 6 cut(s) 441, 816, 1110, 1143, 1327, 1332
FspBI CTAG 2 cut(s) 288, 710
GlaI GCGC 2 cut(s) 168, 1330
GluI GCNGC 6 cut(s) 441, 816, 1110, 1143, 1327, 1332
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 3 cut(s) 233, 391, 702
HapII CCGG 2 cut(s) 30, 273
HhaI GCGC 2 cut(s) 169, 1331
Hin6I GCGC 2 cut(s) 167, 1329
HinP1I GCGC 2 cut(s) 167, 1329
HincII GTYRAC 2 cut(s) 79, 345
HindII GTYRAC 2 cut(s) 79, 345
HinfI GANTC 4 cut(s) 173, 587, 1210, 1355
HpaI GTTAAC 1 cut(s) 345
HpaII CCGG 2 cut(s) 30, 273
HphI GGTGA 3 cut(s) 39, 412, 549
Hpy166II GTNNAC 8 cut(s) 9, 79, 345, 377, 420, 526, 1164, 1209
Hpy188I TCNGA 9 cut(s) 178, 328, 612, 862, 910, 923, 1272, 1285, 1375
Hpy188III TCNNGA 7 cut(s) 98, 596, 967, 1078, 1100, 1303, 1349
Hpy8I GTNNAC 8 cut(s) 9, 79, 345, 377, 420, 526, 1164, 1209
Hpy99I CGWCG 2 cut(s) 74, 80
HpyAV CCTTC 5 cut(s) 257, 411, 884, 905, 1300
HpyCH4III ACNGT 6 cut(s) 349, 360, 569, 796, 1229, 1254
HpyCH4IV ACGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 303, 1032, 1059, 1068, 1337
HpyF3I CTNAG 1 cut(s) 866
HpySE526I ACGT 1 cut(s) 1160
HspAI GCGC 2 cut(s) 167, 1329
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 1129, 1153, 1267
LmnI GCTCC 3 cut(s) 294, 808, 1023
Lsp1109I GCAGC 5 cut(s) 427, 1096, 1154, 1313, 1318
LweI GCATC 4 cut(s) 406, 793, 892, 1245
MaeI CTAG 2 cut(s) 288, 710
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 4 cut(s) 308, 354, 555, 937
MalI GATC 3 cut(s) 1131, 1155, 1269
MboI GATC 3 cut(s) 1129, 1153, 1267
MboII GAAGA 5 cut(s) 473, 775, 1289, 1337, 1364
MflI RGATCY 2 cut(s) 1129, 1153
MhlI GDGCHC 4 cut(s) 299, 805, 951, 1166
MlsI TGGCCA 2 cut(s) 391, 702
MluCI AATT 5 cut(s) 434, 444, 776, 1005, 1279
MluNI TGGCCA 2 cut(s) 391, 702
MlyI GAGTC 1 cut(s) 1204
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 7 cut(s) 7, 98, 603, 624, 928, 984, 1251
Mox20I TGGCCA 2 cut(s) 391, 702
Mph1103I ATGCAT 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 2 cut(s) 391, 702
MseI TTAA 6 cut(s) 114, 344, 362, 477, 746, 825
Msp20I TGGCCA 2 cut(s) 391, 702
MspI CCGG 2 cut(s) 30, 273
MspR9I CCNGG 1 cut(s) 1215
Mva1269I GAATGC 1 cut(s) 1071
MvaI CCWGG 1 cut(s) 1215
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 6 cut(s) 278, 303, 1032, 1059, 1068, 1337
NdeII GATC 3 cut(s) 1129, 1153, 1267
NlaIV GGNNCC 3 cut(s) 296, 1155, 1222
NmuCI GTSAC 3 cut(s) 308, 354, 555
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 5 cut(s) 252, 375, 383, 1066, 1207
PaeI GCATGC 1 cut(s) 1066
PciI ACATGT 3 cut(s) 248, 379, 1203
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PctI GAATGC 1 cut(s) 1071
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 173, 587, 1355
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 6 cut(s) 442, 817, 1111, 1144, 1328, 1333
PleI GAGTC 1 cut(s) 1204
PpsI GAGTC 1 cut(s) 1204
Ppu21I YACGTR 1 cut(s) 1161
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 3 cut(s) 248, 379, 1203
PshBI ATTAAT 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 1213
PspEI GGTNACC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 1213
PspN4I GGNNCC 3 cut(s) 296, 1155, 1222
PspPI GGNCC 3 cut(s) 32, 232, 266
PspPPI RGGWCCY 1 cut(s) 266
PstI CTGCAG 1 cut(s) 982
PsuI RGATCY 2 cut(s) 1129, 1153
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 3 cut(s) 378, 1048, 1202
RsaNI GTAC 3 cut(s) 377, 1047, 1201
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 6 cut(s) 114, 344, 362, 477, 746, 825
SatI GCNGC 6 cut(s) 441, 816, 1110, 1143, 1327, 1332
Sau3AI GATC 3 cut(s) 1129, 1153, 1267
Sau96I GGNCC 3 cut(s) 32, 232, 266
SchI GAGTC 1 cut(s) 1204
ScrFI CCNGG 1 cut(s) 1215
SduI GDGCHC 4 cut(s) 299, 805, 951, 1166
SfaNI GCATC 4 cut(s) 406, 793, 892, 1245
SfcI CTRYAG 1 cut(s) 978
SinI GGWCC 2 cut(s) 32, 266
SphI GCATGC 1 cut(s) 1066
Sse9I AATT 5 cut(s) 434, 444, 776, 1005, 1279
SsiI CCGC 5 cut(s) 85, 340, 739, 815, 1244
SspMI CTAG 2 cut(s) 288, 710
StyD4I CCNGG 1 cut(s) 1213
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 6 cut(s) 349, 360, 569, 796, 1229, 1254
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 4 cut(s) 78, 159, 597, 1358
TasI AATT 5 cut(s) 434, 444, 776, 1005, 1279
TatI WGTACW 3 cut(s) 376, 1046, 1200
TauI GCSGC 1 cut(s) 818
TfiI GAWTC 3 cut(s) 173, 587, 1355
Tru1I TTAA 6 cut(s) 114, 344, 362, 477, 746, 825
Tru9I TTAA 6 cut(s) 114, 344, 362, 477, 746, 825
TscAI CASTG 4 cut(s) 363, 574, 720, 849
TseFI GTSAC 3 cut(s) 308, 354, 555
TseI GCWGC 5 cut(s) 440, 1109, 1142, 1326, 1331
Tsp45I GTSAC 3 cut(s) 308, 354, 555
TspDTI ATGAA 2 cut(s) 276, 675
TspGWI ACGGA 3 cut(s) 58, 684, 788
TspRI CASTG 4 cut(s) 363, 574, 720, 849
VneI GTGCAC 1 cut(s) 1162
VpaK11BI GGWCC 2 cut(s) 32, 266
VspI ATTAAT 1 cut(s) 477
XagI CCTNNNNNAGG 1 cut(s) 286
XapI RAATTY 2 cut(s) 776, 1279
XceI RCATGY 5 cut(s) 252, 375, 383, 1066, 1207
XcmI CCANNNNNNNNNTGG 1 cut(s) 1074
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 2 cut(s) 288, 710
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.