RLG00000026807

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006869
Physical Location & Seq
Forward (+)
6183136 .. 6187793
4658 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026807

Sequence Viewer

Length: 1383 bp
ATGGAGTTCACGGAGGCTTACAAGCAGACCGGGCCTTGCTGCTTCTCGCCCAATGCTCGCTACATTGCCGTCGCCGTCGATTACCGCCTCGTGATTCGCGACACTCTCTCCTTCAAGGTTGTGCAGTTGTTCTCATGCTTGGACAAGATAAGCTACATTGAATGGGCTCTTGATTCTGAGTACATTCTTTGTGGTCTATATAAAAGACCAATGATACAGGCATGGTCGCTGGCCCAACCTGAATGGACATGCAAGATCGATGAAGGTCCTGCCGGCATTGCTTATGCTAGGTGGAGCCCAGATAGCCGTCACATACTTACCACATCAGACTTCCAACTGCGTTTAACAGTTTGGTCTCTCTTAAACACAGCATGTGTACATGTGCAATGGCCTAAGCATCTTTCGAAGGGGGTTTCTTTCACCAAAGATGGGAAATTTGCTGCAATTTGCACGAGGCGCGATTGCAAGGATTATATTAATCTTCTGTCTTGTCAAACTTGGGAAATAATGGGTGTATTTGCTGTTGACACAGTGGACTTGGCTGATACTGAATGGTCACCTGATGACAGTGCAATAGTGATATGGGATTCTTCTCTCGAATATAAGGTTCTGATATACTCCCCAGATGGGAGGTGCCTATTTAAGTATCAAGCATATGAAAGTGGATTGGGGGTAAAAAGTGTTTCATGGTCTCCCTGTGGCCAGTTTCTAGCAGTGGGTAGTTATGATCAGATGTTGCGGGTCTTGAATCACCTGACATGGAAGACTTTTGCAGAATTTATGCATCTATCTACTGTTCGCGCTCCCTGTTGTGCTGCTGTCTTCAAGGAAGTAGATGAGCCACTGCTACTCAATATGTCGGAGTTGTGCTTAACTGATGATTTTGCACCTGAAAATAATGAAGATGCTTCAGAAGGACACGTCAGAGTTAGGTACGAGGTAACGGAAGTGCCCATCACTTTGCCTTTTCAGAAGCCTCCTGGTGACAAACCTAACCCAAAACAAGGAATTGGTCTACTGGCATGGAGCAACGATAGCCAATATATTTGTACTCGCAACGACAGCATGCCATGTATTCTCTGGATATGGGACATCCGCCATCTTGAACTAGCAGCTATCTTGGTGCAGAAAGATCCTATCCGAGCAGCAGTTTGGGACCCTACGTGTACACGTCTTGTTGTTTGCACTGGAAGTTCTCATCTGTACATGTGGACTCCTGGAGGTGCTTATTGTGTGAGTATCCCACTACCACAGTTCAGCATACTTGATCTGAAATGGAATTCAGATGGAAGCTGTCTTTTCCTGAAGGACAAAGAGTCGTTTTGCTGCGCTGCTGTTGCCGTGCTTCCAGAGGAATCAAGTGACTATAGCTCCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

461

Amino Acids

52.0

Weight (kDa)

4.95

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 13 - 192 8e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 633
AccI GTMKAC 1 cut(s) 1015
AccII CGCG 3 cut(s) 99, 459, 801
AciI CCGC 3 cut(s) 85, 739, 1096
AclWI GGATC 1 cut(s) 1127
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 3 cut(s) 434, 776, 1279
AcuI CTGAAG 2 cut(s) 894, 1325
AfaI GTAC 6 cut(s) 182, 378, 935, 1051, 1168, 1205
AfiI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
AflIII ACRYGT 5 cut(s) 379, 919, 1163, 1169, 1206
AgsI TTSAA 5 cut(s) 115, 161, 748, 826, 1106
AhdI GACNNNNNGTC 1 cut(s) 1315
AjiI CACGTC 2 cut(s) 922, 1172
AjnI CCWGG 2 cut(s) 979, 1216
AluBI AGCT 4 cut(s) 153, 1115, 1293, 1371
AluI AGCT 4 cut(s) 153, 1115, 1293, 1371
Alw26I GTCTC 2 cut(s) 360, 696
AlwI GGATC 1 cut(s) 1127
AoxI GGCC 4 cut(s) 32, 231, 389, 700
ApeKI GCWGC 7 cut(s) 39, 440, 815, 1112, 1145, 1326, 1331
ApoI RAATTY 3 cut(s) 434, 776, 1279
AseI ATTAAT 1 cut(s) 477
AspLEI GCGC 3 cut(s) 459, 803, 1331
AspS9I GGNCC 4 cut(s) 32, 232, 266, 1156
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 412, 549, 743, 995
AsuII TTCGAA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 266, 1156
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 1 cut(s) 702
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 2 cut(s) 169, 299
BauI CACGAG 2 cut(s) 89, 451
BbsI GAAGAC 2 cut(s) 770, 814
BbvI GCAGC 7 cut(s) 26, 427, 802, 1124, 1157, 1313, 1318
BccI CCATC 5 cut(s) 422, 620, 962, 1107, 1280
BceAI ACGGC 4 cut(s) 53, 59, 291, 1325
BciT130I CCWGG 2 cut(s) 981, 1218
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 360, 696
BfaI CTAG 3 cut(s) 288, 710, 1109
BfmI CTRYAG 1 cut(s) 1366
BfuI GTATCC 1 cut(s) 1250
BisI GCNGC 7 cut(s) 40, 441, 816, 1113, 1146, 1327, 1332
BlsI GCNGC 7 cut(s) 41, 442, 817, 1114, 1147, 1328, 1333
Bme1390I CCNGG 3 cut(s) 31, 981, 1218
Bme18I GGWCC 2 cut(s) 266, 1156
BmeRI GACNNNNNGTC 1 cut(s) 1315
BmgBI CACGTC 2 cut(s) 922, 1172
BmgT120I GGNCC 4 cut(s) 32, 232, 266, 1156
BmiI GGNNCC 4 cut(s) 296, 635, 1157, 1158
BmrFI CCNGG 3 cut(s) 31, 981, 1218
BmsI GCATC 3 cut(s) 406, 793, 895
BpiI GAAGAC 2 cut(s) 770, 814
BpmI CTGGAG 1 cut(s) 1239
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 31
Bsa29I ATCGAT 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 1164
BsaI GGTCTC 2 cut(s) 360, 696
BsaXI ACNNNNNCTCC 3 cut(s) 92, 122, 1355
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 627, 1004
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 3 cut(s) 703, 1023, 1192
Bse3DI GCAATG 3 cut(s) 63, 276, 392
BseBI CCWGG 2 cut(s) 981, 1218
BseCI ATCGAT 1 cut(s) 258
BseGI GGATG 1 cut(s) 1092
BseLI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
BseMI GCAATG 3 cut(s) 63, 276, 392
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 3 cut(s) 703, 1023, 1192
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 7 cut(s) 26, 427, 802, 1124, 1157, 1313, 1318
BsgI GTGCAG 2 cut(s) 143, 1145
Bsh1236I CGCG 3 cut(s) 99, 459, 801
BshFI GGCC 4 cut(s) 34, 233, 391, 702
BshNI GGYRCC 1 cut(s) 633
BshVI ATCGAT 1 cut(s) 258
BsiSI CCGG 2 cut(s) 30, 273
BslFI GGGAC 2 cut(s) 1103, 1169
BslI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
BsmAI GTCTC 2 cut(s) 360, 696
BsmFI GGGAC 2 cut(s) 1103, 1169
BsnI GGCC 4 cut(s) 34, 233, 391, 702
Bso31I GGTCTC 2 cut(s) 360, 696
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 3 cut(s) 169, 299, 954
Bsp1407I TGTACA 3 cut(s) 376, 1166, 1203
Bsp143I GATC 4 cut(s) 255, 727, 1132, 1267
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 3 cut(s) 85, 739, 1096
BspANI GGCC 4 cut(s) 34, 233, 391, 702
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 258
BspFNI CGCG 3 cut(s) 99, 459, 801
BspLI GGNNCC 4 cut(s) 296, 635, 1157, 1158
BspPI GGATC 1 cut(s) 1127
BspT104I TTCGAA 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 2 cut(s) 360, 696
BsrDI GCAATG 3 cut(s) 63, 276, 392
BsrFI RCCGGY 1 cut(s) 272
BsrGI TGTACA 3 cut(s) 376, 1166, 1203
BsrI ACTGG 3 cut(s) 703, 1023, 1192
BssAI RCCGGY 1 cut(s) 272
BssMI GATC 4 cut(s) 255, 727, 1132, 1267
BssSI CACGAG 2 cut(s) 89, 451
Bst2BI CACGAG 2 cut(s) 89, 451
Bst2UI CCWGG 2 cut(s) 981, 1218
Bst4CI ACNGT 5 cut(s) 349, 532, 569, 796, 1254
BstAUI TGTACA 3 cut(s) 376, 1166, 1203
BstBAI YACGTR 1 cut(s) 1164
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 4 cut(s) 58, 231, 274, 1067
BstDEI CTNAG 2 cut(s) 177, 393
BstEII GGTNACC 1 cut(s) 555
BstF5I GGATG 1 cut(s) 1092
BstFNI CGCG 3 cut(s) 99, 459, 801
BstHHI GCGC 3 cut(s) 459, 803, 1331
BstKTI GATC 4 cut(s) 258, 730, 1135, 1270
BstMAI GTCTC 2 cut(s) 360, 696
BstMBI GATC 4 cut(s) 255, 727, 1132, 1267
BstMWI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 456, 1035, 1062, 1337
BstNI CCWGG 2 cut(s) 981, 1218
BstNSI RCATGY 5 cut(s) 252, 375, 383, 1069, 1210
BstPI GGTNACC 1 cut(s) 555
BstSCI CCNGG 3 cut(s) 29, 979, 1216
BstSFI CTRYAG 1 cut(s) 1366
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 3 cut(s) 99, 459, 801
BstV1I GCAGC 7 cut(s) 26, 427, 802, 1124, 1157, 1313, 1318
BstV2I GAAGAC 2 cut(s) 770, 814
BstX2I RGATCY 1 cut(s) 1132
BstYI RGATCY 1 cut(s) 1132
Bsu15I ATCGAT 1 cut(s) 258
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 4 cut(s) 34, 233, 391, 702
BsuTUI ATCGAT 1 cut(s) 258
BtrI CACGTC 2 cut(s) 922, 1172
BtsCI GGATG 1 cut(s) 1092
BtsI GCAGTG 2 cut(s) 720, 842
BtsIMutI CAGTG 5 cut(s) 537, 574, 720, 842, 1185
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 58, 231, 274, 1067
CfoI GCGC 3 cut(s) 459, 803, 1331
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 4 cut(s) 32, 232, 266, 1156
ClaI ATCGAT 1 cut(s) 258
Csp6I GTAC 6 cut(s) 181, 377, 934, 1050, 1167, 1204
CviQI GTAC 6 cut(s) 181, 377, 934, 1050, 1167, 1204
DdeI CTNAG 2 cut(s) 177, 393
DpnI GATC 4 cut(s) 257, 729, 1134, 1269
DpnII GATC 4 cut(s) 255, 727, 1132, 1267
DriI GACNNNNNGTC 1 cut(s) 1315
EaeI YGGCCR 1 cut(s) 700
Eam1105I GACNNNNNGTC 1 cut(s) 1315
EciI GGCGGA 1 cut(s) 1085
Eco24I GRGCYC 2 cut(s) 169, 299
Eco31I GGTCTC 2 cut(s) 360, 696
Eco47I GGWCC 2 cut(s) 266, 1156
Eco57I CTGAAG 2 cut(s) 894, 1325
Eco91I GGTNACC 1 cut(s) 555
EcoO109I RGGNCCY 2 cut(s) 266, 1156
EcoO65I GGTNACC 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 1279
EcoRII CCWGG 2 cut(s) 979, 1216
EcoT22I ATGCAT 1 cut(s) 786
EcoT38I GRGCYC 2 cut(s) 169, 299
FaqI GGGAC 2 cut(s) 1103, 1169
FauI CCCGC 1 cut(s) 732
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 7 cut(s) 40, 441, 816, 1113, 1146, 1327, 1332
FokI GGATG 1 cut(s) 1079
FriOI GRGCYC 2 cut(s) 169, 299
Fsp4HI GCNGC 7 cut(s) 40, 441, 816, 1113, 1146, 1327, 1332
FspBI CTAG 3 cut(s) 288, 710, 1109
GlaI GCGC 3 cut(s) 458, 802, 1330
GluI GCNGC 7 cut(s) 40, 441, 816, 1113, 1146, 1327, 1332
GsuI CTGGAG 1 cut(s) 1239
HaeIII GGCC 4 cut(s) 34, 233, 391, 702
HapII CCGG 2 cut(s) 30, 273
HhaI GCGC 3 cut(s) 459, 803, 1331
Hin6I GCGC 3 cut(s) 457, 801, 1329
HinP1I GCGC 3 cut(s) 457, 801, 1329
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 7 cut(s) 94, 173, 587, 748, 1213, 1316, 1355
HpaII CCGG 2 cut(s) 30, 273
HphI GGTGA 4 cut(s) 412, 549, 743, 995
Hpy166II GTNNAC 8 cut(s) 9, 377, 526, 535, 1016, 1167, 1169, 1212
Hpy188III TCNNGA 9 cut(s) 91, 98, 170, 596, 745, 1081, 1103, 1303, 1349
Hpy8I GTNNAC 8 cut(s) 9, 377, 526, 535, 1016, 1167, 1169, 1212
Hpy99I CGWCG 2 cut(s) 74, 80
HpyAV CCTTC 5 cut(s) 121, 257, 400, 908, 1300
HpyCH4III ACNGT 5 cut(s) 349, 532, 569, 796, 1254
HpyCH4IV ACGT 3 cut(s) 921, 1163, 1171
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 456, 1035, 1062, 1337
HpyF3I CTNAG 2 cut(s) 177, 393
HpySE526I ACGT 3 cut(s) 921, 1163, 1171
HspAI GCGC 3 cut(s) 457, 801, 1329
KflI GGGWCCC 1 cut(s) 1156
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 4 cut(s) 255, 727, 1132, 1267
LmnI GCTCC 4 cut(s) 294, 808, 1026, 1376
Lsp1109I GCAGC 7 cut(s) 26, 427, 802, 1124, 1157, 1313, 1318
LweI GCATC 3 cut(s) 406, 793, 895
MaeI CTAG 3 cut(s) 288, 710, 1109
MaeII ACGT 3 cut(s) 921, 1163, 1171
MaeIII GTNAC 5 cut(s) 308, 555, 940, 983, 1361
MalI GATC 4 cut(s) 257, 729, 1134, 1269
MboI GATC 4 cut(s) 255, 727, 1132, 1267
MboII GAAGA 5 cut(s) 473, 582, 775, 814, 914
MflI RGATCY 1 cut(s) 1132
MhlI GDGCHC 3 cut(s) 169, 299, 954
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 5 cut(s) 434, 444, 776, 1008, 1279
MluNI TGGCCA 1 cut(s) 702
MlyI GAGTC 2 cut(s) 1207, 1325
MmeI TCCRAC 2 cut(s) 358, 840
MnlI CCTC 8 cut(s) 7, 98, 447, 624, 931, 987, 1214, 1345
Mox20I TGGCCA 1 cut(s) 702
Mph1103I ATGCAT 1 cut(s) 786
MroNI GCCGGC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 702
MseI TTAA 5 cut(s) 344, 362, 477, 642, 872
Msp20I TGGCCA 1 cut(s) 702
MspI CCGG 2 cut(s) 30, 273
MspR9I CCNGG 3 cut(s) 31, 981, 1218
MvaI CCWGG 2 cut(s) 981, 1218
MvnI CGCG 3 cut(s) 99, 459, 801
MwoI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 456, 1035, 1062, 1337
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 655
NdeII GATC 4 cut(s) 255, 727, 1132, 1267
NgoMIV GCCGGC 1 cut(s) 272
NlaIV GGNNCC 4 cut(s) 296, 635, 1157, 1158
NmuCI GTSAC 4 cut(s) 308, 555, 983, 1361
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 5 cut(s) 252, 375, 383, 1069, 1210
NspV TTCGAA 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1069
PciI ACATGT 2 cut(s) 379, 1206
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 5 cut(s) 94, 173, 587, 748, 1355
PfoI TCCNGGA 1 cut(s) 1216
PkrI GCNGC 7 cut(s) 41, 442, 817, 1114, 1147, 1328, 1333
PleI GAGTC 2 cut(s) 1207, 1324
PpsI GAGTC 2 cut(s) 1207, 1324
Ppu21I YACGTR 1 cut(s) 1164
PpuMI RGGWCCY 2 cut(s) 266, 1156
PscI ACATGT 2 cut(s) 379, 1206
PshBI ATTAAT 1 cut(s) 477
Psp5II RGGWCCY 2 cut(s) 266, 1156
Psp6I CCWGG 2 cut(s) 979, 1216
PspEI GGTNACC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 979, 1216
PspN4I GGNNCC 4 cut(s) 296, 635, 1157, 1158
PspPI GGNCC 4 cut(s) 32, 232, 266, 1156
PspPPI RGGWCCY 2 cut(s) 266, 1156
PsuI RGATCY 1 cut(s) 1132
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 6 cut(s) 182, 378, 935, 1051, 1168, 1205
RsaNI GTAC 6 cut(s) 181, 377, 934, 1050, 1167, 1204
SaqAI TTAA 5 cut(s) 344, 362, 477, 642, 872
SatI GCNGC 7 cut(s) 40, 441, 816, 1113, 1146, 1327, 1332
Sau3AI GATC 4 cut(s) 255, 727, 1132, 1267
Sau96I GGNCC 4 cut(s) 32, 232, 266, 1156
SchI GAGTC 2 cut(s) 1207, 1325
ScrFI CCNGG 3 cut(s) 31, 981, 1218
SduI GDGCHC 3 cut(s) 169, 299, 954
SfaNI GCATC 3 cut(s) 406, 793, 895
SfcI CTRYAG 1 cut(s) 1366
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 2 cut(s) 266, 1156
SphI GCATGC 1 cut(s) 1069
Sse9I AATT 5 cut(s) 434, 444, 776, 1008, 1279
SsiI CCGC 3 cut(s) 85, 739, 1096
SspMI CTAG 3 cut(s) 288, 710, 1109
StyD4I CCNGG 3 cut(s) 29, 979, 1216
TaaI ACNGT 5 cut(s) 349, 532, 569, 796, 1254
TaiI ACGT 3 cut(s) 924, 1166, 1174
TaqI TCGA 4 cut(s) 78, 258, 404, 597
TasI AATT 5 cut(s) 434, 444, 776, 1008, 1279
TatI WGTACW 5 cut(s) 180, 376, 1049, 1166, 1203
TfiI GAWTC 5 cut(s) 94, 173, 587, 748, 1355
Tru1I TTAA 5 cut(s) 344, 362, 477, 642, 872
Tru9I TTAA 5 cut(s) 344, 362, 477, 642, 872
TscAI CASTG 5 cut(s) 537, 574, 720, 849, 1192
TseFI GTSAC 4 cut(s) 308, 555, 983, 1361
TseI GCWGC 7 cut(s) 39, 440, 815, 1112, 1145, 1326, 1331
Tsp45I GTSAC 4 cut(s) 308, 555, 983, 1361
TspDTI ATGAA 4 cut(s) 276, 672, 675, 915
TspGWI ACGGA 2 cut(s) 26, 959
TspRI CASTG 5 cut(s) 537, 574, 720, 849, 1192
VpaK11BI GGWCC 2 cut(s) 266, 1156
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 3 cut(s) 434, 776, 1279
XceI RCATGY 5 cut(s) 252, 375, 383, 1069, 1210
XcmI CCANNNNNNNNNTGG 1 cut(s) 1077
XmiI GTMKAC 1 cut(s) 1015
XspI CTAG 3 cut(s) 288, 710, 1109
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.