Rh1DG383200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006869
Physical Location & Seq
Reverse (-)
60397023 .. 60401201
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG383200.1

Sequence Viewer

Length: 1116 bp
ATGGAGTTCACGGAGGCCTACAAGCAGACCGGTCCTTGCTGCTTCTCGCCCAATGCTCGCTACATTGCCGTCGCCGTCGATTACCGCCTCGTGATTCGCGACACTCTCTCCTTCAAGGTTGTGCAGTTGTTCTCATGCTTGGACAAGATAAGCTACATTGAATGGGCTCTTGATTCTGAGTACATTCTTTGTGGTCTATATAAAAGACCAATGATACAGGCATGGTCGCTGGCCCAACCTGAATGGACATGCAAGATAGATGAAGGTCCTGCCGGCATTGCTTATGCTAGGTGGAGCCCAGATAGCCGTCACATACTTACCACATCAGACTTCCAACTGCGTTTAACAGTTTGGTCTCTCTTAAACACAGCATGTGTACATGTGCAATGGCCTAAGCATCTTTCGAAGGGGGTTTCTTTCACCAAAGATGGGAAATTTGCTGCAATTTGCACGAGGCGCGATTGCAAGGATTATATTAATCTTCTGTCTTGTCAAACTTGGGAAATAATGGGTGTATTTGCTGTTGACACAGTGGACTTGGCTGATACTGAATGGTCACCTGATGACAGTGCAATAGTGATATGGGATTCTTCTCTTGAATATAAGGTTCTGATATACTCCCCAGATGGGAGGTGCCTATTTAAGTATCAAGCATATGAAAGTGGATTGGGGGTAAAAAGTGTTTCATGGTCTCCCTGTGGCCAGTTTCTAGCAGTGGGTAGTTATGATCAGATGTTGCGGGTCTTGAATCACCTGACATGGAAGACTTTTGCAGAATTTATGCATCTATCTACTGTTCGTGCTCCCTGTTGTGCTGCTGTCTTCAAGGAAGTAGATGAGCCGCTGCTACTCAATATGTCGGAGTTATGCTTAACTGATGATTTTGCACCTGAAAATAATGAAGATGCTTCAGAAGGACACGTCAGAGTTAGGTACGAGGTAACAGAAGTGCCCATCACTTTGCCTTTTCAGAAGCCTCCTGGTGACAAACCTAACCCAAAACAAGGAATTGGTCTACTGGCATGGAGCAACGATAGCCAATATATTTGTACTCGCAACGACAGCATGCCATGTCCTCTAATGATGGGACTTATTGAGATGAATCCATTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

371

Amino Acids

42.1

Weight (kDa)

5.03

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 13 - 192 4.8e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 633
AccI GTMKAC 1 cut(s) 1015
AccII CGCG 2 cut(s) 99, 459
AciI CCGC 3 cut(s) 85, 739, 842
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 434, 776
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 4 cut(s) 182, 378, 935, 1051
AfiI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
AflIII ACRYGT 2 cut(s) 379, 919
AgeI ACCGGT 1 cut(s) 29
AgsI TTSAA 5 cut(s) 115, 161, 599, 748, 826
AjiI CACGTC 1 cut(s) 922
AjnI CCWGG 1 cut(s) 979
AluBI AGCT 1 cut(s) 153
AluI AGCT 1 cut(s) 153
Alw21I GWGCWC 1 cut(s) 805
Alw26I GTCTC 2 cut(s) 360, 696
AoxI GGCC 4 cut(s) 15, 231, 389, 700
ApeKI GCWGC 4 cut(s) 39, 440, 815, 844
ApoI RAATTY 2 cut(s) 434, 776
AseI ATTAAT 1 cut(s) 477
AsiGI ACCGGT 1 cut(s) 29
Asp700I GAANNNNTTC 1 cut(s) 1106
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 32, 232, 266
AsuHPI GGTGA 4 cut(s) 412, 549, 743, 995
AsuII TTCGAA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 32, 266
BaeGI GKGCMC 1 cut(s) 954
BalI TGGCCA 1 cut(s) 702
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 2 cut(s) 169, 299
BauI CACGAG 2 cut(s) 89, 451
BbsI GAAGAC 2 cut(s) 770, 814
Bbv12I GWGCWC 1 cut(s) 805
BbvI GCAGC 4 cut(s) 26, 427, 802, 831
BccI CCATC 4 cut(s) 422, 620, 962, 1078
BceAI ACGGC 3 cut(s) 53, 59, 291
BciT130I CCWGG 1 cut(s) 981
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 2 cut(s) 360, 696
BfaI CTAG 2 cut(s) 288, 710
BisI GCNGC 5 cut(s) 40, 441, 816, 842, 845
BlsI GCNGC 5 cut(s) 41, 442, 817, 843, 846
Bme1390I CCNGG 1 cut(s) 981
Bme18I GGWCC 2 cut(s) 32, 266
BmgBI CACGTC 1 cut(s) 922
BmgT120I GGNCC 3 cut(s) 32, 232, 266
BmiI GGNNCC 2 cut(s) 296, 635
BmrFI CCNGG 1 cut(s) 981
BmsI GCATC 3 cut(s) 406, 793, 895
BpiI GAAGAC 2 cut(s) 770, 814
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 404
BsaI GGTCTC 2 cut(s) 360, 696
BsaWI WCCGGW 1 cut(s) 29
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 627, 1004
Bse118I RCCGGY 2 cut(s) 29, 272
Bse1I ACTGG 2 cut(s) 703, 1023
Bse3DI GCAATG 3 cut(s) 63, 276, 392
BseBI CCWGG 1 cut(s) 981
BseLI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
BseMI GCAATG 3 cut(s) 63, 276, 392
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 703, 1023
BseSI GKGCMC 1 cut(s) 954
BseXI GCAGC 4 cut(s) 26, 427, 802, 831
BsgI GTGCAG 1 cut(s) 143
Bsh1236I CGCG 2 cut(s) 99, 459
BshFI GGCC 4 cut(s) 17, 233, 391, 702
BshNI GGYRCC 1 cut(s) 633
BshTI ACCGGT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 805
BsiSI CCGG 2 cut(s) 30, 273
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 3 cut(s) 429, 627, 1004
BsmAI GTCTC 2 cut(s) 360, 696
BsmFI GGGAC 1 cut(s) 1101
BsnI GGCC 4 cut(s) 17, 233, 391, 702
Bso31I GGTCTC 2 cut(s) 360, 696
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 4 cut(s) 169, 299, 805, 954
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 1 cut(s) 727
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 3 cut(s) 85, 739, 842
BspANI GGCC 4 cut(s) 17, 233, 391, 702
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 2 cut(s) 99, 459
BspLI GGNNCC 2 cut(s) 296, 635
BspT104I TTCGAA 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 633
BspTNI GGTCTC 2 cut(s) 360, 696
BsrDI GCAATG 3 cut(s) 63, 276, 392
BsrFI RCCGGY 2 cut(s) 29, 272
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 2 cut(s) 703, 1023
BssAI RCCGGY 2 cut(s) 29, 272
BssMI GATC 1 cut(s) 727
BssSI CACGAG 2 cut(s) 89, 451
Bst2BI CACGAG 2 cut(s) 89, 451
Bst2UI CCWGG 1 cut(s) 981
Bst4CI ACNGT 4 cut(s) 349, 532, 569, 796
BstAUI TGTACA 1 cut(s) 376
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 4 cut(s) 58, 231, 274, 1067
BstDEI CTNAG 2 cut(s) 177, 393
BstEII GGTNACC 1 cut(s) 555
BstFNI CGCG 2 cut(s) 99, 459
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 730
BstMAI GTCTC 2 cut(s) 360, 696
BstMBI GATC 1 cut(s) 727
BstMWI GCNNNNNNNGC 5 cut(s) 278, 303, 456, 1035, 1062
BstNI CCWGG 1 cut(s) 981
BstNSI RCATGY 4 cut(s) 252, 375, 383, 1069
BstPI GGTNACC 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 954
BstUI CGCG 2 cut(s) 99, 459
BstV1I GCAGC 4 cut(s) 26, 427, 802, 831
BstV2I GAAGAC 2 cut(s) 770, 814
BsuRI GGCC 4 cut(s) 17, 233, 391, 702
BtrI CACGTC 1 cut(s) 922
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 3 cut(s) 537, 574, 720
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 58, 231, 274, 1067
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 2 cut(s) 29, 272
Cfr13I GGNCC 3 cut(s) 32, 232, 266
Csp6I GTAC 4 cut(s) 181, 377, 934, 1050
CspAI ACCGGT 1 cut(s) 29
CviQI GTAC 4 cut(s) 181, 377, 934, 1050
DdeI CTNAG 2 cut(s) 177, 393
DpnI GATC 1 cut(s) 729
DpnII GATC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 700
Eco147I AGGCCT 1 cut(s) 17
Eco24I GRGCYC 2 cut(s) 169, 299
Eco31I GGTCTC 2 cut(s) 360, 696
Eco47I GGWCC 2 cut(s) 32, 266
Eco57I CTGAAG 1 cut(s) 894
Eco91I GGTNACC 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 979
EcoT22I ATGCAT 1 cut(s) 786
EcoT38I GRGCYC 2 cut(s) 169, 299
FaqI GGGAC 1 cut(s) 1101
FauI CCCGC 1 cut(s) 732
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 1015
Fnu4HI GCNGC 5 cut(s) 40, 441, 816, 842, 845
FriOI GRGCYC 2 cut(s) 169, 299
Fsp4HI GCNGC 5 cut(s) 40, 441, 816, 842, 845
FspBI CTAG 2 cut(s) 288, 710
GlaI GCGC 1 cut(s) 458
GluI GCNGC 5 cut(s) 40, 441, 816, 842, 845
HaeIII GGCC 4 cut(s) 17, 233, 391, 702
HapII CCGG 2 cut(s) 30, 273
HhaI GCGC 1 cut(s) 459
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 5 cut(s) 94, 173, 587, 748, 1102
HpaII CCGG 2 cut(s) 30, 273
HphI GGTGA 4 cut(s) 412, 549, 743, 995
Hpy166II GTNNAC 5 cut(s) 9, 377, 526, 535, 1016
Hpy188I TCNGA 8 cut(s) 178, 328, 612, 732, 862, 913, 926, 972
Hpy188III TCNNGA 5 cut(s) 91, 98, 170, 596, 745
Hpy8I GTNNAC 5 cut(s) 9, 377, 526, 535, 1016
Hpy99I CGWCG 2 cut(s) 74, 80
HpyAV CCTTC 4 cut(s) 121, 257, 400, 908
HpyCH4III ACNGT 4 cut(s) 349, 532, 569, 796
HpyCH4IV ACGT 1 cut(s) 921
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 303, 456, 1035, 1062
HpyF3I CTNAG 2 cut(s) 177, 393
HpySE526I ACGT 1 cut(s) 921
HspAI GCGC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 1 cut(s) 727
LmnI GCTCC 3 cut(s) 294, 808, 1026
Lsp1109I GCAGC 4 cut(s) 26, 427, 802, 831
LweI GCATC 3 cut(s) 406, 793, 895
MaeI CTAG 2 cut(s) 288, 710
MaeII ACGT 1 cut(s) 921
MaeIII GTNAC 4 cut(s) 308, 555, 940, 983
MalI GATC 1 cut(s) 729
MboI GATC 1 cut(s) 727
MboII GAAGA 5 cut(s) 473, 582, 775, 814, 914
MhlI GDGCHC 4 cut(s) 169, 299, 805, 954
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 4 cut(s) 434, 444, 776, 1008
MluNI TGGCCA 1 cut(s) 702
MmeI TCCRAC 2 cut(s) 358, 840
MnlI CCTC 7 cut(s) 7, 98, 447, 624, 931, 987, 1086
Mox20I TGGCCA 1 cut(s) 702
Mph1103I ATGCAT 1 cut(s) 786
MroNI GCCGGC 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 1106
MscI TGGCCA 1 cut(s) 702
MseI TTAA 5 cut(s) 344, 362, 477, 642, 872
Msp20I TGGCCA 1 cut(s) 702
MspA1I CMGCKG 1 cut(s) 844
MspI CCGG 2 cut(s) 30, 273
MspR9I CCNGG 1 cut(s) 981
MvaI CCWGG 1 cut(s) 981
MvnI CGCG 2 cut(s) 99, 459
MwoI GCNNNNNNNGC 5 cut(s) 278, 303, 456, 1035, 1062
NaeI GCCGGC 1 cut(s) 274
NdeI CATATG 1 cut(s) 655
NdeII GATC 1 cut(s) 727
NgoMIV GCCGGC 1 cut(s) 272
NlaIV GGNNCC 2 cut(s) 296, 635
NmuCI GTSAC 3 cut(s) 308, 555, 983
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 786
NspI RCATGY 4 cut(s) 252, 375, 383, 1069
NspV TTCGAA 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1069
PceI AGGCCT 1 cut(s) 17
PciI ACATGT 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 1106
PfeI GAWTC 5 cut(s) 94, 173, 587, 748, 1102
PinAI ACCGGT 1 cut(s) 29
PkrI GCNGC 5 cut(s) 41, 442, 817, 843, 846
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 979
PspEI GGTNACC 1 cut(s) 555
PspGI CCWGG 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 296, 635
PspPI GGNCC 3 cut(s) 32, 232, 266
PspPPI RGGWCCY 1 cut(s) 266
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 4 cut(s) 182, 378, 935, 1051
RsaNI GTAC 4 cut(s) 181, 377, 934, 1050
SaqAI TTAA 5 cut(s) 344, 362, 477, 642, 872
SatI GCNGC 5 cut(s) 40, 441, 816, 842, 845
Sau3AI GATC 1 cut(s) 727
Sau96I GGNCC 3 cut(s) 32, 232, 266
ScrFI CCNGG 1 cut(s) 981
SduI GDGCHC 4 cut(s) 169, 299, 805, 954
SfaNI GCATC 3 cut(s) 406, 793, 895
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 2 cut(s) 32, 266
SphI GCATGC 1 cut(s) 1069
Sse9I AATT 4 cut(s) 434, 444, 776, 1008
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 3 cut(s) 85, 739, 842
SspMI CTAG 2 cut(s) 288, 710
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 979
TaaI ACNGT 4 cut(s) 349, 532, 569, 796
TaiI ACGT 1 cut(s) 924
TaqI TCGA 2 cut(s) 78, 404
TasI AATT 4 cut(s) 434, 444, 776, 1008
TatI WGTACW 3 cut(s) 180, 376, 1049
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 5 cut(s) 94, 173, 587, 748, 1102
Tru1I TTAA 5 cut(s) 344, 362, 477, 642, 872
Tru9I TTAA 5 cut(s) 344, 362, 477, 642, 872
TscAI CASTG 3 cut(s) 537, 574, 720
TseFI GTSAC 3 cut(s) 308, 555, 983
TseI GCWGC 4 cut(s) 39, 440, 815, 844
Tsp45I GTSAC 3 cut(s) 308, 555, 983
TspDTI ATGAA 5 cut(s) 276, 672, 675, 915, 1115
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 537, 574, 720
VpaK11BI GGWCC 2 cut(s) 32, 266
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 2 cut(s) 434, 776
XceI RCATGY 4 cut(s) 252, 375, 383, 1069
XmiI GTMKAC 1 cut(s) 1015
XmnI GAANNNNTTC 1 cut(s) 1106
XspI CTAG 2 cut(s) 288, 710
Zsp2I ATGCAT 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.