Rroxscaffold_4G00284060

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006869
Physical Location & Seq
Forward (+)
5792772 .. 5796869
4098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284060.1

Sequence Viewer

Length: 1293 bp
ATGGAGTTCACAGAGGCTTACAAGCAGACCGGGCCTTGCTGCTTCTCGCCCAATGCTCGCTACATTGCCGTCGCCGTCGATTACCGCCTCGTGATTCGCGACACTCTCTCCTTCAAGGTTGTGCAGTTGTTCTCATGCTTGGACAAGATAAGCTACATTGAATGGGCTCTTGATTCTGAGTACATTCTTTGTGGTCTATATAAAAGACCAATGATACAGGCATGGTCGCTGGCCCAACCTGAATGGACATGCAAGATAGATGAAGGTCCTGCCGGCATTGCTTATGCTAGGTGGAGCCCAGATAGCCCATCTATCGAAGGGGTTTCTTTCACCAAAGATGGGAAATTTGCTGCAATTTGCACGAGGCGCGATTGCAAGGATTATATTAATCTTCTGTCTTGTCAAACTTGGGAAATAATGGGTGTATTTGCTGTTGACACAGTGGACTTGGCTGATACTGAATGGTCACCTGATGACAGTGCAATAGTGATATGGGATTCTTCTCTGGAATATAAGGTTCTGATATACTCCCCAGATGGGAGGTGCCTATTTAAGTATCAAGCATATGAAAGTGGATTGGGGGTAAAAAGTGTTTCATGGTCTCCCTGTGGCCAGTTTCTAGCAGTGGGTAGTTATGATCAGATGTTGCGGGTCTTGAATCACCTGACATGGAAGACTTTTGCAGAATTTATGCATCTATCTACTGTTCGCGCTCCCTGTTGTGCTGCTGTCTTCAAGGAAGTAGATGAGCCACTGCTACTCAATATGTCGGAGTTATGCTTAACTGATGATTTTGCACCTGAAAATAATGAAGATGCTTCAGAAGGACACATCAGAGTTAGGTACGAGGTAACAGAAGTGCCCATCACTTTGCCTTTTCAGAAGCCTCCTGGTGACAAACCTAACCCAAAACAAGGAATTGGTCTACTGGCATGGAGCAATGATAGCCAATATATTTGTACTCGCAACGACAGCATGCCATGTATTCTCTGGATATGGGACATCCGCCATCTTGAACTAGCAGCTATCTTGGTGCAGAAAGATCCTATCCGAGCAGCAGTATGGGACCCTACATGTACACGTCTTGTTGTTTGCACCGGAAGTTCTCATCTGTACATGTGGACTCCTGGAGGTGCTTATTGTGTGAGTATCCCACTACCACAGTTCAGCATACTTGATCTGAAATGGAATTCAGATGGAAGCTGTCTTTTCCTGAAGGACAAAGAGTCATTTTGCTGCGCTGCTGTTGCCGTGCTTCCAGAGGAATCAAGTGACTATAGCTCCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

430

Amino Acids

48.43

Weight (kDa)

4.65

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 108 - 233 1.3e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 543
AccI GTMKAC 1 cut(s) 925
AccII CGCG 3 cut(s) 99, 369, 711
AciI CCGC 3 cut(s) 85, 649, 1006
AclWI GGATC 1 cut(s) 1037
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 3 cut(s) 344, 686, 1189
AcuI CTGAAG 2 cut(s) 804, 1235
AfaI GTAC 5 cut(s) 182, 845, 961, 1078, 1115
AfiI CCNNNNNNNGG 3 cut(s) 339, 537, 914
AflIII ACRYGT 3 cut(s) 1073, 1079, 1116
AgsI TTSAA 5 cut(s) 115, 161, 658, 736, 1016
AhdI GACNNNNNGTC 1 cut(s) 1225
AjiI CACGTC 1 cut(s) 1082
AjnI CCWGG 2 cut(s) 889, 1126
AluBI AGCT 4 cut(s) 153, 1025, 1203, 1281
AluI AGCT 4 cut(s) 153, 1025, 1203, 1281
Alw26I GTCTC 1 cut(s) 606
AlwI GGATC 1 cut(s) 1037
AoxI GGCC 3 cut(s) 32, 231, 610
ApeKI GCWGC 7 cut(s) 39, 350, 725, 1022, 1055, 1236, 1241
ApoI RAATTY 3 cut(s) 344, 686, 1189
AseI ATTAAT 1 cut(s) 387
AspLEI GCGC 3 cut(s) 369, 713, 1241
AspS9I GGNCC 4 cut(s) 32, 232, 266, 1066
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 4 cut(s) 322, 459, 653, 905
AvaII GGWCC 2 cut(s) 266, 1066
BaeGI GKGCMC 1 cut(s) 864
BalI TGGCCA 1 cut(s) 612
BanI GGYRCC 1 cut(s) 543
BanII GRGCYC 2 cut(s) 169, 299
BauI CACGAG 2 cut(s) 89, 361
BbsI GAAGAC 2 cut(s) 680, 724
BbvI GCAGC 7 cut(s) 26, 337, 712, 1034, 1067, 1223, 1228
BccI CCATC 6 cut(s) 316, 332, 530, 872, 1017, 1190
BceAI ACGGC 3 cut(s) 53, 59, 1235
BciT130I CCWGG 2 cut(s) 891, 1128
BciVI GTATCC 1 cut(s) 1160
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 3 cut(s) 288, 620, 1019
BfmI CTRYAG 1 cut(s) 1276
BfuI GTATCC 1 cut(s) 1160
BisI GCNGC 7 cut(s) 40, 351, 726, 1023, 1056, 1237, 1242
BlsI GCNGC 7 cut(s) 41, 352, 727, 1024, 1057, 1238, 1243
Bme1390I CCNGG 3 cut(s) 31, 891, 1128
Bme18I GGWCC 2 cut(s) 266, 1066
BmeRI GACNNNNNGTC 1 cut(s) 1225
BmgBI CACGTC 1 cut(s) 1082
BmgT120I GGNCC 4 cut(s) 32, 232, 266, 1066
BmiI GGNNCC 4 cut(s) 296, 545, 1067, 1068
BmrFI CCNGG 3 cut(s) 31, 891, 1128
BmsI GCATC 2 cut(s) 703, 805
BpiI GAAGAC 2 cut(s) 680, 724
BpmI CTGGAG 1 cut(s) 1149
BpuMI CCSGG 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 606
BsaWI WCCGGW 1 cut(s) 1097
BsaXI ACNNNNNCTCC 3 cut(s) 92, 122, 1265
Bsc4I CCNNNNNNNGG 3 cut(s) 339, 537, 914
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 613, 933
Bse3DI GCAATG 3 cut(s) 63, 276, 946
BseBI CCWGG 2 cut(s) 891, 1128
BseGI GGATG 1 cut(s) 1002
BseLI CCNNNNNNNGG 3 cut(s) 339, 537, 914
BseMI GCAATG 3 cut(s) 63, 276, 946
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 613, 933
BseSI GKGCMC 1 cut(s) 864
BseXI GCAGC 7 cut(s) 26, 337, 712, 1034, 1067, 1223, 1228
BsgI GTGCAG 2 cut(s) 143, 1055
Bsh1236I CGCG 3 cut(s) 99, 369, 711
BshFI GGCC 3 cut(s) 34, 233, 612
BshNI GGYRCC 1 cut(s) 543
BsiSI CCGG 3 cut(s) 30, 273, 1098
BslFI GGGAC 2 cut(s) 1013, 1079
BslI CCNNNNNNNGG 3 cut(s) 339, 537, 914
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 1013, 1079
BsnI GGCC 3 cut(s) 34, 233, 612
Bso31I GGTCTC 1 cut(s) 606
Bsp1286I GDGCHC 3 cut(s) 169, 299, 864
Bsp1407I TGTACA 2 cut(s) 1076, 1113
Bsp143I GATC 3 cut(s) 637, 1042, 1177
Bsp68I TCGCGA 1 cut(s) 99
BspACI CCGC 3 cut(s) 85, 649, 1006
BspANI GGCC 3 cut(s) 34, 233, 612
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 3 cut(s) 99, 369, 711
BspLI GGNNCC 4 cut(s) 296, 545, 1067, 1068
BspPI GGATC 1 cut(s) 1037
BspT107I GGYRCC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 606
BsrDI GCAATG 3 cut(s) 63, 276, 946
BsrFI RCCGGY 1 cut(s) 272
BsrGI TGTACA 2 cut(s) 1076, 1113
BsrI ACTGG 2 cut(s) 613, 933
BssAI RCCGGY 1 cut(s) 272
BssMI GATC 3 cut(s) 637, 1042, 1177
BssSI CACGAG 2 cut(s) 89, 361
Bst2BI CACGAG 2 cut(s) 89, 361
Bst2UI CCWGG 2 cut(s) 891, 1128
Bst4CI ACNGT 4 cut(s) 442, 479, 706, 1164
BstAUI TGTACA 2 cut(s) 1076, 1113
BstC8I GCNNGC 4 cut(s) 58, 231, 274, 977
BstDEI CTNAG 1 cut(s) 177
BstEII GGTNACC 1 cut(s) 465
BstF5I GGATG 1 cut(s) 1002
BstFNI CGCG 3 cut(s) 99, 369, 711
BstHHI GCGC 3 cut(s) 369, 713, 1241
BstKTI GATC 3 cut(s) 640, 1045, 1180
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 3 cut(s) 637, 1042, 1177
BstMWI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 366, 945, 972, 1247
BstNI CCWGG 2 cut(s) 891, 1128
BstNSI RCATGY 4 cut(s) 252, 979, 1077, 1120
BstPI GGTNACC 1 cut(s) 465
BstSCI CCNGG 3 cut(s) 29, 889, 1126
BstSFI CTRYAG 1 cut(s) 1276
BstSLI GKGCMC 1 cut(s) 864
BstUI CGCG 3 cut(s) 99, 369, 711
BstV1I GCAGC 7 cut(s) 26, 337, 712, 1034, 1067, 1223, 1228
BstV2I GAAGAC 2 cut(s) 680, 724
BstX2I RGATCY 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 1160
BsuRI GGCC 3 cut(s) 34, 233, 612
BtrI CACGTC 1 cut(s) 1082
BtsCI GGATG 1 cut(s) 1002
BtsI GCAGTG 2 cut(s) 630, 752
BtsIMutI CAGTG 4 cut(s) 447, 484, 630, 752
BtuMI TCGCGA 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 58, 231, 274, 977
CfoI GCGC 3 cut(s) 369, 713, 1241
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 4 cut(s) 32, 232, 266, 1066
Csp6I GTAC 5 cut(s) 181, 844, 960, 1077, 1114
CviQI GTAC 5 cut(s) 181, 844, 960, 1077, 1114
DdeI CTNAG 1 cut(s) 177
DpnI GATC 3 cut(s) 639, 1044, 1179
DpnII GATC 3 cut(s) 637, 1042, 1177
DriI GACNNNNNGTC 1 cut(s) 1225
EaeI YGGCCR 1 cut(s) 610
Eam1105I GACNNNNNGTC 1 cut(s) 1225
EciI GGCGGA 1 cut(s) 995
Eco24I GRGCYC 2 cut(s) 169, 299
Eco31I GGTCTC 1 cut(s) 606
Eco47I GGWCC 2 cut(s) 266, 1066
Eco57I CTGAAG 2 cut(s) 804, 1235
Eco91I GGTNACC 1 cut(s) 465
EcoO109I RGGNCCY 2 cut(s) 266, 1066
EcoO65I GGTNACC 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 1189
EcoRII CCWGG 2 cut(s) 889, 1126
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 2 cut(s) 169, 299
FaqI GGGAC 2 cut(s) 1013, 1079
FauI CCCGC 1 cut(s) 642
FauNDI CATATG 1 cut(s) 565
FbaI TGATCA 1 cut(s) 637
FblI GTMKAC 1 cut(s) 925
Fnu4HI GCNGC 7 cut(s) 40, 351, 726, 1023, 1056, 1237, 1242
FokI GGATG 1 cut(s) 989
FriOI GRGCYC 2 cut(s) 169, 299
Fsp4HI GCNGC 7 cut(s) 40, 351, 726, 1023, 1056, 1237, 1242
FspBI CTAG 3 cut(s) 288, 620, 1019
GlaI GCGC 3 cut(s) 368, 712, 1240
GluI GCNGC 7 cut(s) 40, 351, 726, 1023, 1056, 1237, 1242
GsuI CTGGAG 1 cut(s) 1149
HaeIII GGCC 3 cut(s) 34, 233, 612
HapII CCGG 3 cut(s) 30, 273, 1098
HhaI GCGC 3 cut(s) 369, 713, 1241
Hin6I GCGC 3 cut(s) 367, 711, 1239
HinP1I GCGC 3 cut(s) 367, 711, 1239
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 7 cut(s) 94, 173, 497, 658, 1123, 1226, 1265
HpaII CCGG 3 cut(s) 30, 273, 1098
HphI GGTGA 4 cut(s) 322, 459, 653, 905
Hpy166II GTNNAC 6 cut(s) 9, 436, 445, 926, 1079, 1122
Hpy188III TCNNGA 9 cut(s) 91, 98, 170, 506, 655, 991, 1013, 1213, 1259
Hpy8I GTNNAC 6 cut(s) 9, 436, 445, 926, 1079, 1122
Hpy99I CGWCG 2 cut(s) 74, 80
HpyAV CCTTC 5 cut(s) 121, 257, 311, 818, 1210
HpyCH4III ACNGT 4 cut(s) 442, 479, 706, 1164
HpyCH4IV ACGT 1 cut(s) 1081
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 366, 945, 972, 1247
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 1081
HspAI GCGC 3 cut(s) 367, 711, 1239
KflI GGGWCCC 1 cut(s) 1066
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 3 cut(s) 637, 1042, 1177
LmnI GCTCC 4 cut(s) 294, 718, 936, 1286
Lsp1109I GCAGC 7 cut(s) 26, 337, 712, 1034, 1067, 1223, 1228
LweI GCATC 2 cut(s) 703, 805
MaeI CTAG 3 cut(s) 288, 620, 1019
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 4 cut(s) 465, 850, 893, 1271
MalI GATC 3 cut(s) 639, 1044, 1179
MboI GATC 3 cut(s) 637, 1042, 1177
MboII GAAGA 5 cut(s) 383, 492, 685, 724, 824
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 3 cut(s) 169, 299, 864
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 5 cut(s) 344, 354, 686, 918, 1189
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 2 cut(s) 1117, 1235
MmeI TCCRAC 1 cut(s) 750
MnlI CCTC 8 cut(s) 7, 98, 357, 534, 841, 897, 1124, 1255
Mox20I TGGCCA 1 cut(s) 612
Mph1103I ATGCAT 1 cut(s) 696
MroNI GCCGGC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 612
MseI TTAA 3 cut(s) 387, 552, 782
Msp20I TGGCCA 1 cut(s) 612
MspI CCGG 3 cut(s) 30, 273, 1098
MspR9I CCNGG 3 cut(s) 31, 891, 1128
MvaI CCWGG 2 cut(s) 891, 1128
MvnI CGCG 3 cut(s) 99, 369, 711
MwoI GCNNNNNNNGC 7 cut(s) 31, 278, 303, 366, 945, 972, 1247
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 637, 1042, 1177
NgoMIV GCCGGC 1 cut(s) 272
NlaIV GGNNCC 4 cut(s) 296, 545, 1067, 1068
NmuCI GTSAC 3 cut(s) 465, 893, 1271
NruI TCGCGA 1 cut(s) 99
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 4 cut(s) 252, 979, 1077, 1120
PaeI GCATGC 1 cut(s) 979
PciI ACATGT 2 cut(s) 1073, 1116
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 5 cut(s) 94, 173, 497, 658, 1265
PfoI TCCNGGA 1 cut(s) 1126
PkrI GCNGC 7 cut(s) 41, 352, 727, 1024, 1057, 1238, 1243
PleI GAGTC 2 cut(s) 1117, 1234
PpsI GAGTC 2 cut(s) 1117, 1234
PpuMI RGGWCCY 2 cut(s) 266, 1066
PscI ACATGT 2 cut(s) 1073, 1116
PshBI ATTAAT 1 cut(s) 387
Psp5II RGGWCCY 2 cut(s) 266, 1066
Psp6I CCWGG 2 cut(s) 889, 1126
PspEI GGTNACC 1 cut(s) 465
PspGI CCWGG 2 cut(s) 889, 1126
PspN4I GGNNCC 4 cut(s) 296, 545, 1067, 1068
PspPI GGNCC 4 cut(s) 32, 232, 266, 1066
PspPPI RGGWCCY 2 cut(s) 266, 1066
PsuI RGATCY 1 cut(s) 1042
RruI TCGCGA 1 cut(s) 99
RsaI GTAC 5 cut(s) 182, 845, 961, 1078, 1115
RsaNI GTAC 5 cut(s) 181, 844, 960, 1077, 1114
SaqAI TTAA 3 cut(s) 387, 552, 782
SatI GCNGC 7 cut(s) 40, 351, 726, 1023, 1056, 1237, 1242
Sau3AI GATC 3 cut(s) 637, 1042, 1177
Sau96I GGNCC 4 cut(s) 32, 232, 266, 1066
SchI GAGTC 2 cut(s) 1117, 1235
ScrFI CCNGG 3 cut(s) 31, 891, 1128
SduI GDGCHC 3 cut(s) 169, 299, 864
SfaNI GCATC 2 cut(s) 703, 805
SfcI CTRYAG 1 cut(s) 1276
SinI GGWCC 2 cut(s) 266, 1066
SphI GCATGC 1 cut(s) 979
Sse9I AATT 5 cut(s) 344, 354, 686, 918, 1189
SsiI CCGC 3 cut(s) 85, 649, 1006
SspMI CTAG 3 cut(s) 288, 620, 1019
StyD4I CCNGG 3 cut(s) 29, 889, 1126
TaaI ACNGT 4 cut(s) 442, 479, 706, 1164
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 2 cut(s) 78, 315
TasI AATT 5 cut(s) 344, 354, 686, 918, 1189
TatI WGTACW 4 cut(s) 180, 959, 1076, 1113
TfiI GAWTC 5 cut(s) 94, 173, 497, 658, 1265
Tru1I TTAA 3 cut(s) 387, 552, 782
Tru9I TTAA 3 cut(s) 387, 552, 782
TscAI CASTG 4 cut(s) 447, 484, 630, 759
TseFI GTSAC 3 cut(s) 465, 893, 1271
TseI GCWGC 7 cut(s) 39, 350, 725, 1022, 1055, 1236, 1241
Tsp45I GTSAC 3 cut(s) 465, 893, 1271
TspDTI ATGAA 4 cut(s) 276, 582, 585, 825
TspRI CASTG 4 cut(s) 447, 484, 630, 759
VpaK11BI GGWCC 2 cut(s) 266, 1066
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 3 cut(s) 344, 686, 1189
XceI RCATGY 4 cut(s) 252, 979, 1077, 1120
XcmI CCANNNNNNNNNTGG 1 cut(s) 987
XmiI GTMKAC 1 cut(s) 925
XspI CTAG 3 cut(s) 288, 620, 1019
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.