MD00G1007700.v1.1

BES1 BZR1 homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006018
Physical Location & Seq
Reverse (-)
1033907 .. 1037289
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1007700.v1.1.491

Sequence Viewer

Length: 996 bp
ATGATGACGTCGGGAACCAGAATGCCGACGTGGAAGGAGAGAGAGAACAACAAGAAGCGGGAGCGGCGGCGCCGTGCCATCGCTGCGAAGATCTACTCCGGGCTGAGGATGTACGGAAACTACAAGCTCCCTAAGCACTGCGACAACAACGAGGTCCTCAAAGCTCTCTGCAACGAAGCCGGATGGACCGTGGAGGAAGACGGCACCACTTACCGAAAGGGATGCAAACCCGTGGATCGCATGGACATAATGGGGGGTTCTACATCAGCAAGTCCGTGTTCATCATACCAGCCAAGTCCGTGTGCGTCCTACAATCCAAGTCCCGGAGCATCCTACAATCCAAGTCCTGCCTCATCTTCCTTCCCAAGTCCTGCTCGCTACCCTGCAAATGGAAACGGTAATGCTGATGCCAATTCTCTGATCCCATGGCTTAAAACGCTCTCGTCAGGCTCATCATCCGCCACGTCCAAGCTCCCCCAACTCTTTGTTCAAGGGGGTTCCATAAGTGCTCCAGTTACCCCACCACTGAGCTCCCCAACATGTCGAACTCCTCGAACCAAGAGCGATTGGGACGAAGCAGTGGCTGGCTCAAACTGGGCAGGGCAGCATTATCCTTTCCTGCCTTCGTCCACCCCACCAAGCCCCGGTCGCCAGGTTCTGCCTGATTCAGGATGGCTTGCTGGACTGCAAATCCCCCAGAGTGGCCCATCATCGCCAACATTCAGCCTTGTCTCGAGAAACCCTTTTAACTTGAAGGAGGCTTTGTCAGGTGGAGGGTCTCGGATGTGGACTCCCGGGCAAAGTGGAACATGTTCCCCGGCAGTTGCTGCAAGTATTGATCACACCGGAGATGTTCCAATGTCCGACGGGATGGCAGCAGAGTTTGCATTTGGCAGTAACACAACAGGACTAGTAAAGCCATGGGAAGGTGAGAGGATTGAGGAATGCGTATCAGATGATCTCGAACTTACGCTCGGGAGCACTCGAACAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
BES1_N

Protein Analysis

332

Amino Acids

35.28

Weight (kDa)

8.74

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BES1_N PF05687 5 - 147 1e-65 BES1/BZR1 plant transcription factor, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 11
AccB1I GGYRCC 2 cut(s) 69, 203
AccBSI CCGCTC 1 cut(s) 64
AciI CCGC 4 cut(s) 58, 64, 67, 459
AclWI GGATC 2 cut(s) 243, 415
AcyI GRCGYC 2 cut(s) 8, 70
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 7 cut(s) 105, 323, 389, 644, 668, 701, 926
AflIII ACRYGT 2 cut(s) 539, 809
AgsI TTSAA 2 cut(s) 491, 754
AhlI ACTAGT 1 cut(s) 910
AjiI CACGTC 2 cut(s) 30, 465
AjnI CCWGG 1 cut(s) 651
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 4 cut(s) 127, 164, 472, 531
AluI AGCT 4 cut(s) 127, 164, 472, 531
Alw21I GWGCWC 3 cut(s) 511, 533, 983
Alw26I GTCTC 2 cut(s) 736, 783
AlwI GGATC 2 cut(s) 243, 415
AlwNI CAGNNNCTG 3 cut(s) 584, 658, 827
Ama87I CYCGRG 3 cut(s) 733, 794, 974
AoxI GGCC 1 cut(s) 703
ApeKI GCWGC 4 cut(s) 83, 604, 827, 875
Asp700I GAANNNNTTC 1 cut(s) 811
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 3 cut(s) 154, 186, 704
AsuC2I CCSGG 6 cut(s) 100, 324, 645, 795, 796, 818
AsuHPI GGTGA 1 cut(s) 941
AvaI CYCGRG 3 cut(s) 733, 794, 974
AvaII GGWCC 2 cut(s) 154, 186
BanI GGYRCC 2 cut(s) 69, 203
BanII GRGCYC 1 cut(s) 533
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 3 cut(s) 511, 533, 983
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 4 cut(s) 70, 616, 814, 887
BccI CCATC 5 cut(s) 86, 177, 666, 715, 865
BceAI ACGGC 2 cut(s) 57, 217
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BciT130I CCWGG 1 cut(s) 653
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 6 cut(s) 100, 324, 645, 795, 796, 818
BcoDI GTCTC 2 cut(s) 736, 783
BcuI ACTAGT 1 cut(s) 910
BfaI CTAG 1 cut(s) 911
BfoI RGCGCY 1 cut(s) 73
BglII AGATCT 1 cut(s) 90
BisI GCNGC 6 cut(s) 65, 68, 84, 605, 828, 876
BlsI GCNGC 6 cut(s) 66, 69, 85, 606, 829, 877
Bme1390I CCNGG 7 cut(s) 100, 324, 645, 653, 795, 796, 818
Bme18I GGWCC 2 cut(s) 154, 186
BmeT110I CYCGRG 3 cut(s) 733, 794, 974
BmgBI CACGTC 2 cut(s) 30, 465
BmgT120I GGNCC 3 cut(s) 154, 186, 704
BmiI GGNNCC 4 cut(s) 16, 71, 205, 499
BmrFI CCNGG 7 cut(s) 100, 324, 645, 653, 795, 796, 818
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 3 cut(s) 212, 338, 397
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 2 cut(s) 104, 132
BpuMI CCSGG 6 cut(s) 100, 324, 645, 795, 796, 818
BsaHI GRCGYC 2 cut(s) 8, 70
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 7 cut(s) 189, 231, 425, 643, 794, 816, 920
BsaWI WCCGGW 1 cut(s) 845
BsaXI ACNNNNNCTCC 2 cut(s) 749, 779
Bsc4I CCNNNNNNNGG 7 cut(s) 105, 323, 389, 644, 668, 701, 926
Bse1I ACTGG 2 cut(s) 512, 599
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 7 cut(s) 189, 231, 425, 643, 794, 816, 920
BseGI GGATG 8 cut(s) 114, 188, 227, 329, 455, 677, 789, 876
BseLI CCNNNNNNNGG 7 cut(s) 105, 323, 389, 644, 668, 701, 926
BseMII CTCAG 2 cut(s) 95, 518
BseNI ACTGG 2 cut(s) 512, 599
BseRI GAGGAG 1 cut(s) 540
BseXI GCAGC 4 cut(s) 70, 616, 814, 887
Bsh1285I CGRYCG 1 cut(s) 649
BshFI GGCC 1 cut(s) 705
BshNI GGYRCC 2 cut(s) 69, 203
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 3 cut(s) 511, 533, 983
BsiHKCI CYCGRG 3 cut(s) 733, 794, 974
BsiSI CCGG 7 cut(s) 99, 180, 324, 645, 795, 818, 846
BslFI GGGAC 2 cut(s) 306, 584
BslI CCNNNNNNNGG 7 cut(s) 105, 323, 389, 644, 668, 701, 926
BsmAI GTCTC 2 cut(s) 736, 783
BsmFI GGGAC 2 cut(s) 306, 584
BsmI GAATGC 2 cut(s) 27, 950
BsnI GGCC 1 cut(s) 705
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 3 cut(s) 733, 794, 974
Bsp1286I GDGCHC 3 cut(s) 511, 533, 983
Bsp143I GATC 5 cut(s) 90, 235, 420, 838, 958
Bsp19I CCATGG 2 cut(s) 425, 920
BspACI CCGC 4 cut(s) 58, 64, 67, 459
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 2 cut(s) 96, 519
BspLI GGNNCC 4 cut(s) 16, 71, 205, 499
BspPI GGATC 2 cut(s) 243, 415
BspT107I GGYRCC 2 cut(s) 69, 203
BspTNI GGTCTC 1 cut(s) 783
BsrBI CCGCTC 1 cut(s) 64
BsrI ACTGG 2 cut(s) 512, 599
BssECI CCNNGG 7 cut(s) 189, 231, 425, 643, 794, 816, 920
BssMI GATC 5 cut(s) 90, 235, 420, 838, 958
BssNI GRCGYC 2 cut(s) 8, 70
BssT1I CCWWGG 2 cut(s) 425, 920
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 2 cut(s) 190, 398
BstACI GRCGYC 2 cut(s) 8, 70
BstAPI GCANNNNNTGC 2 cut(s) 827, 884
BstC8I GCNNGC 3 cut(s) 376, 586, 678
BstDEI CTNAG 3 cut(s) 104, 132, 527
BstDSI CCRYGG 4 cut(s) 189, 231, 425, 920
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 8 cut(s) 114, 188, 227, 329, 455, 677, 789, 876
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 5 cut(s) 93, 238, 423, 841, 961
BstMAI GTCTC 2 cut(s) 736, 783
BstMBI GATC 5 cut(s) 90, 235, 420, 838, 958
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 7 cut(s) 64, 83, 133, 436, 648, 827, 884
BstNI CCWGG 1 cut(s) 653
BstNSI RCATGY 2 cut(s) 543, 813
BstSCI CCNGG 7 cut(s) 98, 322, 643, 651, 793, 794, 816
BstV1I GCAGC 4 cut(s) 70, 616, 814, 887
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 1 cut(s) 705
BtgI CCRYGG 4 cut(s) 189, 231, 425, 920
BtgZI GCGATG 2 cut(s) 64, 696
BtrI CACGTC 2 cut(s) 30, 465
BtsCI GGATG 8 cut(s) 114, 188, 227, 329, 455, 677, 789, 876
BtsI GCAGTG 2 cut(s) 136, 585
BtsIMutI CAGTG 3 cut(s) 136, 524, 585
Cac8I GCNNGC 3 cut(s) 376, 586, 678
CaiI CAGNNNCTG 3 cut(s) 584, 658, 827
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 3 cut(s) 154, 186, 704
Cfr9I CCCGGG 1 cut(s) 794
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 112
CviAII CATG 5 cut(s) 241, 426, 540, 810, 921
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 3 cut(s) 104, 132, 527
DinI GGCGCC 1 cut(s) 71
DpnI GATC 5 cut(s) 92, 237, 422, 840, 960
DpnII GATC 5 cut(s) 90, 235, 420, 838, 958
EciI GGCGGA 1 cut(s) 448
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 425, 920
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 1 cut(s) 783
Eco47I GGWCC 2 cut(s) 154, 186
Eco53kI GAGCTC 1 cut(s) 531
Eco88I CYCGRG 3 cut(s) 733, 794, 974
EcoICRI GAGCTC 1 cut(s) 531
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 425, 920
EcoT38I GRGCYC 1 cut(s) 533
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
ErhI CCWWGG 2 cut(s) 425, 920
FaeI CATG 5 cut(s) 244, 429, 543, 813, 924
FaiI YATR 8 cut(s) 242, 248, 286, 427, 503, 541, 811, 922
FaqI GGGAC 2 cut(s) 306, 584
FatI CATG 5 cut(s) 240, 425, 539, 809, 920
FauI CCCGC 1 cut(s) 51
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 6 cut(s) 65, 68, 84, 605, 828, 876
FokI GGATG 8 cut(s) 121, 195, 234, 316, 442, 684, 796, 883
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 6 cut(s) 65, 68, 84, 605, 828, 876
FspBI CTAG 1 cut(s) 911
GlaI GCGC 1 cut(s) 71
GluI GCNGC 6 cut(s) 65, 68, 84, 605, 828, 876
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 1 cut(s) 705
HapII CCGG 7 cut(s) 99, 180, 324, 645, 795, 818, 846
HgaI GACGC 1 cut(s) 294
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 2 cut(s) 8, 70
Hin1II CATG 5 cut(s) 244, 429, 543, 813, 924
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HinfI GANTC 2 cut(s) 665, 790
HpaII CCGG 7 cut(s) 99, 180, 324, 645, 795, 818, 846
HphI GGTGA 1 cut(s) 941
Hpy166II GTNNAC 2 cut(s) 630, 789
Hpy188I TCNGA 4 cut(s) 420, 783, 865, 955
Hpy188III TCNNGA 6 cut(s) 12, 669, 733, 735, 962, 976
Hpy8I GTNNAC 2 cut(s) 630, 789
Hpy99I CGWCG 3 cut(s) 13, 31, 869
HpyAV CCTTC 5 cut(s) 28, 370, 633, 748, 920
HpyCH4III ACNGT 2 cut(s) 190, 398
HpyCH4IV ACGT 3 cut(s) 8, 29, 464
HpyCH4V TGCA 6 cut(s) 171, 225, 386, 688, 830, 887
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 83, 133, 436, 648, 827, 884
HpyF3I CTNAG 3 cut(s) 104, 132, 527
HpySE526I ACGT 3 cut(s) 8, 29, 464
Hsp92I GRCGYC 2 cut(s) 8, 70
Hsp92II CATG 5 cut(s) 244, 429, 543, 813, 924
HspAI GCGC 1 cut(s) 70
KasI GGCGCC 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 5 cut(s) 90, 235, 420, 838, 958
LmnI GCTCC 7 cut(s) 61, 132, 326, 477, 514, 536, 978
Lsp1109I GCAGC 4 cut(s) 70, 616, 814, 887
LweI GCATC 3 cut(s) 212, 338, 397
MaeI CTAG 1 cut(s) 911
MaeII ACGT 3 cut(s) 8, 29, 464
MaeIII GTNAC 2 cut(s) 514, 896
MalI GATC 5 cut(s) 92, 237, 422, 840, 960
MbiI CCGCTC 1 cut(s) 64
MboI GATC 5 cut(s) 90, 235, 420, 838, 958
MboII GAAGA 3 cut(s) 100, 209, 348
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 3 cut(s) 511, 533, 983
MluCI AATT 1 cut(s) 412
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 811
MseI TTAA 2 cut(s) 432, 747
MspI CCGG 7 cut(s) 99, 180, 324, 645, 795, 818, 846
MspR9I CCNGG 7 cut(s) 100, 324, 645, 653, 795, 796, 818
Mva1269I GAATGC 2 cut(s) 27, 950
MvaI CCWGG 1 cut(s) 653
MwoI GCNNNNNNNGC 7 cut(s) 64, 83, 133, 436, 648, 827, 884
NarI GGCGCC 1 cut(s) 70
NciI CCSGG 6 cut(s) 100, 324, 645, 795, 796, 818
NcoI CCATGG 2 cut(s) 425, 920
NdeII GATC 5 cut(s) 90, 235, 420, 838, 958
NlaIII CATG 5 cut(s) 244, 429, 543, 813, 924
NlaIV GGNNCC 4 cut(s) 16, 71, 205, 499
NspI RCATGY 2 cut(s) 543, 813
PaeR7I CTCGAG 1 cut(s) 733
PciI ACATGT 2 cut(s) 539, 809
PcsI WCGNNNNNNNCGW 1 cut(s) 550
PctI GAATGC 2 cut(s) 27, 950
PdmI GAANNNNTTC 1 cut(s) 811
PfeI GAWTC 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 6 cut(s) 66, 69, 85, 606, 829, 877
PleI GAGTC 1 cut(s) 784
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 154
PscI ACATGT 2 cut(s) 539, 809
Psp124BI GAGCTC 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 651
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 4 cut(s) 16, 71, 205, 499
PspPI GGNCC 3 cut(s) 154, 186, 704
PspPPI RGGWCCY 1 cut(s) 154
PstNI CAGNNNCTG 3 cut(s) 584, 658, 827
PsuI RGATCY 1 cut(s) 90
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SacI GAGCTC 1 cut(s) 533
SaqAI TTAA 2 cut(s) 432, 747
SatI GCNGC 6 cut(s) 65, 68, 84, 605, 828, 876
Sau3AI GATC 5 cut(s) 90, 235, 420, 838, 958
Sau96I GGNCC 3 cut(s) 154, 186, 704
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 7 cut(s) 100, 324, 645, 653, 795, 796, 818
SduI GDGCHC 3 cut(s) 511, 533, 983
SfaNI GCATC 3 cut(s) 212, 338, 397
SfoI GGCGCC 1 cut(s) 71
Sfr274I CTCGAG 1 cut(s) 733
SinI GGWCC 2 cut(s) 154, 186
SlaI CTCGAG 1 cut(s) 733
SmaI CCCGGG 1 cut(s) 796
SmlI CTYRAG 1 cut(s) 733
SmoI CTYRAG 1 cut(s) 733
SpeI ACTAGT 1 cut(s) 910
Sse9I AATT 1 cut(s) 412
SsiI CCGC 4 cut(s) 58, 64, 67, 459
SspDI GGCGCC 1 cut(s) 69
SspMI CTAG 1 cut(s) 911
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 7 cut(s) 98, 322, 643, 651, 793, 794, 816
StyI CCWWGG 2 cut(s) 425, 920
TaaI ACNGT 2 cut(s) 190, 398
TaiI ACGT 3 cut(s) 11, 32, 467
TaqI TCGA 5 cut(s) 544, 553, 734, 963, 985
TasI AATT 1 cut(s) 412
TauI GCSGC 2 cut(s) 67, 70
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 2 cut(s) 432, 747
Tru9I TTAA 2 cut(s) 432, 747
TscAI CASTG 3 cut(s) 143, 531, 585
TseI GCWGC 4 cut(s) 83, 604, 827, 875
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 3 cut(s) 129, 264, 288
TspMI CCCGGG 1 cut(s) 794
TspRI CASTG 3 cut(s) 143, 531, 585
VpaK11BI GGWCC 2 cut(s) 154, 186
XagI CCTNNNNNAGG 1 cut(s) 666
XceI RCATGY 2 cut(s) 543, 813
XhoI CTCGAG 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 794
XmnI GAANNNNTTC 1 cut(s) 811
XspI CTAG 1 cut(s) 911
ZraI GACGTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.