Rorug04G0460200

Las17-binding protein actin regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002221
Physical Location & Seq
Forward (+)
61246496 .. 61252445
5950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0460200.1

Sequence Viewer

Length: 678 bp
ATGATCAATCCAGCTAAACCAGAGAGTGACTGTTGGAGTGACGATAGTGACAGCGATAAGAAATCTGTTTCTTTTAGCAATACTACTGCCAGCACATATGCTACTTCAGATGATTCTGTTTCTACCAGTGACCATGAGATTGAGGCCGATTGCCTTCACCACCTGTATTGCCACTACAACGAAACATCTAGTCCTCATGATAGAGTTCCTTTGACCCTCAAGATCAATGAATTAGCTCAAATTTATCCTGGTTTGATGAAGTTTCAAAGCTTGGAGCTCTCTCCTTATAGCTGGATGGCTGTTGCTTGGTATCCTATCTATCAAATTCCAGTTACAAGGAATGTGAAGGAATTATCTGCATGTTTCATCACTTATCACACATTATCATCTTTTCAAGGCCTCCATAGTACCATATCAGAGAATAATGATAAGTTTCAATGCATTGATACATTGGGAAAGGATCATGGAGGCAAGACGCTAGAAAGAGATGAAAGCAAAGGTGAAATGGCTCTTCCTCCTTTTGCAGCGGCCACCTATAAGATGCATGGCGAACTTTGGACAACTCCTGAGACTTCGGATAAGGAAAAGATTGGCTTTTATCTGAACGCTGCAAATTCCTGGTTGAAGCATCGCAAATTCCAGCATCATGACTTTAATTTCTTCATATCTCGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE Ysc84

Protein Analysis

225

Amino Acids

25.7

Weight (kDa)

5.69

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 7 - 221 3e-60 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 527
AclWI GGATC 1 cut(s) 468
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 4 cut(s) 240, 324, 613, 635
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 1 cut(s) 409
AgsI TTSAA 4 cut(s) 266, 395, 437, 625
AjnI CCWGG 2 cut(s) 247, 617
AluBI AGCT 5 cut(s) 14, 236, 270, 277, 291
AluI AGCT 5 cut(s) 14, 236, 270, 277, 291
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 1 cut(s) 468
AoxI GGCC 3 cut(s) 144, 397, 528
ApeKI GCWGC 2 cut(s) 524, 608
ApoI RAATTY 4 cut(s) 240, 324, 613, 635
AsuHPI GGTGA 2 cut(s) 149, 512
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 536, 595
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 2 cut(s) 249, 619
BciVI GTATCC 1 cut(s) 321
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 2 cut(s) 189, 479
BfuI GTATCC 1 cut(s) 321
BisI GCNGC 3 cut(s) 525, 528, 609
BlsI GCNGC 3 cut(s) 526, 529, 610
Bme1390I CCNGG 2 cut(s) 249, 619
BmrFI CCNGG 2 cut(s) 249, 619
BmsI GCATC 3 cut(s) 531, 637, 652
BpuEI CTTGAG 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 126, 329
BseBI CCWGG 2 cut(s) 249, 619
BseGI GGATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 558
BseNI ACTGG 2 cut(s) 126, 329
BseXI GCAGC 2 cut(s) 536, 595
BshFI GGCC 3 cut(s) 146, 399, 530
BsiHKAI GWGCWC 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 563
BsnI GGCC 3 cut(s) 146, 399, 530
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 3 cut(s) 3, 222, 460
BspACI CCGC 1 cut(s) 527
BspANI GGCC 3 cut(s) 146, 399, 530
BspCNI CTCAG 1 cut(s) 559
BspHI TCATGA 2 cut(s) 196, 646
BspPI GGATC 1 cut(s) 468
BspQI GCTCTTC 1 cut(s) 516
BsrI ACTGG 2 cut(s) 126, 329
BssMI GATC 3 cut(s) 3, 222, 460
Bst2UI CCWGG 2 cut(s) 249, 619
Bst4CI ACNGT 1 cut(s) 32
Bst6I CTCTTC 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 1 cut(s) 567
BstF5I GGATG 1 cut(s) 300
BstKTI GATC 3 cut(s) 6, 225, 463
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 3 cut(s) 3, 222, 460
BstNI CCWGG 2 cut(s) 249, 619
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 247, 617
BstV1I GCAGC 2 cut(s) 536, 595
BsuI GTATCC 1 cut(s) 321
BsuRI GGCC 3 cut(s) 146, 399, 530
BtgZI GCGATG 1 cut(s) 614
BtsCI GGATG 1 cut(s) 300
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 91
CciI TCATGA 2 cut(s) 196, 646
CseI GACGC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 408
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviAII CATG 6 cut(s) 134, 197, 360, 464, 545, 647
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 1 cut(s) 567
DpnI GATC 3 cut(s) 5, 224, 462
DpnII GATC 3 cut(s) 3, 222, 460
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 247, 617
EcoT22I ATGCAT 2 cut(s) 443, 546
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 6 cut(s) 137, 200, 363, 467, 548, 650
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FatI CATG 6 cut(s) 133, 196, 359, 463, 544, 646
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 525, 528, 609
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 3 cut(s) 525, 528, 609
FspBI CTAG 2 cut(s) 189, 479
GluI GCNGC 3 cut(s) 525, 528, 609
HaeIII GGCC 3 cut(s) 146, 399, 530
HgaI GACGC 1 cut(s) 484
Hin1II CATG 6 cut(s) 137, 200, 363, 467, 548, 650
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 1 cut(s) 113
HphI GGTGA 2 cut(s) 149, 512
Hpy188I TCNGA 4 cut(s) 109, 418, 577, 603
Hpy188III TCNNGA 4 cut(s) 197, 220, 566, 647
HpyAV CCTTC 2 cut(s) 164, 340
HpyCH4III ACNGT 1 cut(s) 32
HpyCH4V TGCA 5 cut(s) 359, 441, 524, 544, 611
HpyF3I CTNAG 1 cut(s) 567
Hsp92II CATG 6 cut(s) 137, 200, 363, 467, 548, 650
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 222, 460
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 2 cut(s) 536, 595
LweI GCATC 3 cut(s) 531, 637, 652
MaeI CTAG 2 cut(s) 189, 479
MaeIII GTNAC 5 cut(s) 26, 38, 47, 128, 331
MalI GATC 3 cut(s) 5, 224, 462
MboI GATC 3 cut(s) 3, 222, 460
MboII GAAGA 2 cut(s) 503, 652
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 7 cut(s) 230, 240, 324, 350, 613, 635, 655
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 6 cut(s) 136, 204, 227, 410, 461, 525
Mph1103I ATGCAT 2 cut(s) 443, 546
MseI TTAA 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 527
MspR9I CCNGG 2 cut(s) 249, 619
MvaI CCWGG 2 cut(s) 249, 619
NdeI CATATG 1 cut(s) 97
NdeII GATC 3 cut(s) 3, 222, 460
NlaIII CATG 6 cut(s) 137, 200, 363, 467, 548, 650
NmuCI GTSAC 4 cut(s) 26, 38, 47, 128
NsiI ATGCAT 2 cut(s) 443, 546
NspI RCATGY 1 cut(s) 363
PagI TCATGA 2 cut(s) 196, 646
PceI AGGCCT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 516
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 3 cut(s) 526, 529, 610
Psp124BI GAGCTC 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 247, 617
PspGI CCWGG 2 cut(s) 247, 617
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SacI GAGCTC 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 654
SatI GCNGC 3 cut(s) 525, 528, 609
Sau3AI GATC 3 cut(s) 3, 222, 460
ScrFI CCNGG 2 cut(s) 249, 619
SduI GDGCHC 1 cut(s) 279
SetI ASST 9 cut(s) 16, 165, 238, 272, 279, 293, 502, 536, 677
SfaNI GCATC 3 cut(s) 531, 637, 652
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 7 cut(s) 230, 240, 324, 350, 613, 635, 655
SseBI AGGCCT 1 cut(s) 399
SsiI CCGC 1 cut(s) 527
SspMI CTAG 2 cut(s) 189, 479
SstI GAGCTC 1 cut(s) 279
StuI AGGCCT 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 247, 617
TaaI ACNGT 1 cut(s) 32
TasI AATT 7 cut(s) 230, 240, 324, 350, 613, 635, 655
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 654
Tru9I TTAA 1 cut(s) 654
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 4 cut(s) 26, 38, 47, 128
TseI GCWGC 2 cut(s) 524, 608
Tsp45I GTSAC 4 cut(s) 26, 38, 47, 128
TspDTI ATGAA 5 cut(s) 243, 272, 355, 504, 652
TspRI CASTG 1 cut(s) 133
XapI RAATTY 4 cut(s) 240, 324, 613, 635
XceI RCATGY 1 cut(s) 363
XspI CTAG 2 cut(s) 189, 479
Zsp2I ATGCAT 2 cut(s) 443, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.