FvH4_6g27900

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002221
Physical Location & Seq
Reverse (-)
21468363 .. 21472179
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27900.t1

Sequence Viewer

Length: 1320 bp
ATGGATGGGTCTCAAGGTGGTTCGAATGCACCGGCTCCTTTTCTTTCGAAAACATATGACTTGGTGGATGATCCTTCAACCAACCACGTGGTTTCTTGGAGTGAAACTGGTGGTAGTTTTGTAGTTTGGGATCCCACAGAGTTTGCCAAGGATTTGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTTGTCAGGCAGCTTAATACATATGGGTTCAGGAAGGTTGATCCTGAGCAGTGGGAGTTTGCAAATGAGGAATTCTTGAGAGGAGGAAGACATCTACTGAAGAATATTCACCGCCGCAAGCCAATTCATAGTCATTCCATGCAGAATCACGAGTACTCTTCAGTTCCTTTAAGCGAGACAGAAAGGGAAGAATATGAAAAGAAAATCAATAGACTGATTAATGATAAGAACTTGCTTGAGCTAGAGCTACAAAGACATCAAAGGGAGAATCAGGAGTTTGAATTCCAAATGAAATTGTTAAATGAACAATTGCAAAAGATGGAACTTCGGCAGAGGCAATATACGACCTTCCTAGCTCAAGTATTGCAGAAGCCAGGGTTTGCCTCTTTACTTATGCAACAATCGGAAAATCATAACAAAAAGAGAAGACTATTGAAACCTAACCTATTTCCTGACGAGTTCAGTATGGAAGGTTTGAATTTGAATTCACAGAAAGAGAACCTGGATTCAGTTTCAACTTCCACATCAAAGTCAGAGCGGCTTGAGAAAATGGAGTCTTCCTTGAACTTTTGGGAAGATTTTATACGTGGGATTGGAGAAGCTATGCCTGAAGAAGTAAATGATATTGGCCCTATGTCTGAGGCCTCTCCCATCATTGTTTCAGAAATACAAGACCTTGGCATGAATAGCAGGCCGTGTTCACCTATATCGAACTTATCTTCACCGACTTCAATGAATGTTCATTCATCCCCAGAGGTGGCTGGGTCTGCAAATCTTTTTGACATCCTCGCTATCTCATCAATGTATCAATCTGGTGATTTCAGGCCTAAATCTTCAGGACTTGATATGAACTCTAAGCCTGATAGTGCACCTGCAATTGAAGCTCCAAAAGAAAGGGTAGTGGAAATGACAGCCGCTGAGCCTGCTGCAGCAAATGATGTGTTTTGGGAGCAATGTTTAACAGAGACCCCTGGTTTAGAAGATGCACAGGAAGTACAATCGGAAAGAAGGGATGGTGATGGCGGAGCAAGTGATACCAATGCAGCTGCTCAGAAAAAGCTTTGGTGGAACACAGATAATGTAGATAACTTTACAAACCAAATAGGGCGCCTCACTCCAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

440

Amino Acids

49.78

Weight (kDa)

4.99

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 14 - 103 1.4e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1072
Acc36I ACCTGC 1 cut(s) 1072
AccB1I GGYRCC 1 cut(s) 1299
AccBSI CCGCTC 1 cut(s) 730
AciI CCGC 5 cut(s) 304, 307, 730, 1107, 1215
AclWI GGATC 4 cut(s) 65, 125, 138, 227
AcsI RAATTY 4 cut(s) 263, 473, 670, 676
AcuI CTGAAG 4 cut(s) 311, 336, 822, 1011
AcvI CACGTG 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 1300
AfaI GTAC 2 cut(s) 347, 1188
AfiI CCNNNNNNNGG 1 cut(s) 949
AjnI CCWGG 3 cut(s) 565, 693, 1162
AluBI AGCT 9 cut(s) 205, 433, 439, 548, 794, 1076, 1238, 1252, 1313
AluI AGCT 9 cut(s) 205, 433, 439, 548, 794, 1076, 1238, 1252, 1313
Alw21I GWGCWC 1 cut(s) 1063
Alw26I GTCTC 3 cut(s) 15, 362, 1151
Alw44I GTGCAC 1 cut(s) 1059
AlwI GGATC 4 cut(s) 65, 125, 138, 227
AlwNI CAGNNNCTG 1 cut(s) 1109
AoxI GGCC 4 cut(s) 820, 834, 884, 1016
ApaLI GTGCAC 1 cut(s) 1059
ApeKI GCWGC 5 cut(s) 202, 1118, 1121, 1235, 1238
ApoI RAATTY 4 cut(s) 263, 473, 670, 676
AseI ATTAAT 1 cut(s) 411
AspLEI GCGC 1 cut(s) 1302
AspS9I GGNCC 1 cut(s) 821
AsuHPI GGTGA 5 cut(s) 293, 885, 906, 1019, 1220
AsuII TTCGAA 2 cut(s) 23, 47
BaeGI GKGCMC 1 cut(s) 1063
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 1299
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BauI CACGAG 1 cut(s) 341
BbrPI CACGTG 1 cut(s) 88
BbsI GAAGAC 3 cut(s) 286, 625, 741
Bbv12I GWGCWC 1 cut(s) 1063
BbvI GCAGC 5 cut(s) 214, 1105, 1133, 1225, 1247
BccI CCATC 4 cut(s) 505, 851, 1199, 1205
BceAI ACGGC 1 cut(s) 871
BciT130I CCWGG 3 cut(s) 567, 695, 1164
BcoDI GTCTC 3 cut(s) 15, 362, 1151
BfaI CTAG 2 cut(s) 434, 545
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 1303
BfuAI ACCTGC 1 cut(s) 1072
BisI GCNGC 8 cut(s) 203, 307, 731, 1107, 1119, 1122, 1236, 1239
BlpI GCTNAGC 1 cut(s) 1110
BlsI GCNGC 8 cut(s) 204, 308, 732, 1108, 1120, 1123, 1237, 1240
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 567, 695, 1164
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 36, 132, 1301
BmrFI CCNGG 3 cut(s) 567, 695, 1164
BmsI GCATC 1 cut(s) 1165
BpiI GAAGAC 3 cut(s) 286, 625, 741
BpmI CTGGAG 1 cut(s) 1293
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 2 cut(s) 23, 47
BpuEI CTTGAG 4 cut(s) 289, 449, 534, 755
BsaAI YACGTR 2 cut(s) 88, 779
BsaHI GRCGYC 1 cut(s) 1300
BsaI GGTCTC 2 cut(s) 15, 1151
BsaJI CCNNGG 4 cut(s) 147, 566, 868, 1162
Bsc4I CCNNNNNNNGG 1 cut(s) 949
Bse118I RCCGGY 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 1151
BseBI CCWGG 3 cut(s) 567, 695, 1164
BseDI CCNNGG 4 cut(s) 147, 566, 868, 1162
BseGI GGATG 5 cut(s) 10, 73, 938, 975, 1210
BseLI CCNNNNNNNGG 1 cut(s) 949
BseMI GCAATG 1 cut(s) 1151
BseMII CTCAG 4 cut(s) 228, 822, 1101, 1256
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 1063
BseXI GCAGC 5 cut(s) 214, 1105, 1133, 1225, 1247
BseYI CCCAGC 1 cut(s) 953
BshFI GGCC 4 cut(s) 822, 836, 886, 1018
BshNI GGYRCC 1 cut(s) 1299
BsiHKAI GWGCWC 1 cut(s) 1063
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 949
BsmAI GTCTC 3 cut(s) 15, 362, 1151
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 4 cut(s) 822, 836, 886, 1018
Bso31I GGTCTC 2 cut(s) 15, 1151
Bsp119I TTCGAA 2 cut(s) 23, 47
Bsp1286I GDGCHC 1 cut(s) 1063
Bsp143I GATC 3 cut(s) 70, 130, 232
Bsp1720I GCTNAGC 1 cut(s) 1110
BspACI CCGC 5 cut(s) 304, 307, 730, 1107, 1215
BspANI GGCC 4 cut(s) 822, 836, 886, 1018
BspCNI CTCAG 4 cut(s) 229, 823, 1102, 1255
BspLI GGNNCC 3 cut(s) 36, 132, 1301
BspMAI CTGCAG 1 cut(s) 1123
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 4 cut(s) 65, 125, 138, 227
BspT104I TTCGAA 2 cut(s) 23, 47
BspT107I GGYRCC 1 cut(s) 1299
BspTNI GGTCTC 2 cut(s) 15, 1151
BsrBI CCGCTC 1 cut(s) 730
BsrDI GCAATG 1 cut(s) 1151
BsrFI RCCGGY 1 cut(s) 31
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 31
BssECI CCNNGG 4 cut(s) 147, 566, 868, 1162
BssMI GATC 3 cut(s) 70, 130, 232
BssNI GRCGYC 1 cut(s) 1300
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 2 cut(s) 147, 868
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 3 cut(s) 567, 695, 1164
Bst6I CTCTTC 1 cut(s) 355
BstACI GRCGYC 1 cut(s) 1300
BstBAI YACGTR 2 cut(s) 88, 779
BstBI TTCGAA 2 cut(s) 23, 47
BstC8I GCNNGC 3 cut(s) 311, 884, 1116
BstDEI CTNAG 5 cut(s) 237, 831, 1047, 1110, 1242
BstF5I GGATG 5 cut(s) 10, 73, 938, 975, 1210
BstH2I RGCGCY 1 cut(s) 1303
BstHHI GCGC 1 cut(s) 1302
BstKTI GATC 3 cut(s) 73, 133, 235
BstMAI GTCTC 3 cut(s) 15, 362, 1151
BstMBI GATC 3 cut(s) 70, 130, 232
BstMWI GCNNNNNNNGC 4 cut(s) 879, 959, 1073, 1115
BstNI CCWGG 3 cut(s) 567, 695, 1164
BstSCI CCNGG 3 cut(s) 565, 693, 1162
BstSFI CTRYAG 1 cut(s) 1119
BstSLI GKGCMC 1 cut(s) 1063
BstV1I GCAGC 5 cut(s) 214, 1105, 1133, 1225, 1247
BstV2I GAAGAC 3 cut(s) 286, 625, 741
BstX2I RGATCY 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 130
BsuRI GGCC 4 cut(s) 822, 836, 886, 1018
BtsCI GGATG 5 cut(s) 10, 73, 938, 975, 1210
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 248
BveI ACCTGC 1 cut(s) 1072
Cac8I GCNNGC 3 cut(s) 311, 884, 1116
CaiI CAGNNNCTG 1 cut(s) 1109
CfoI GCGC 1 cut(s) 1302
Cfr10I RCCGGY 1 cut(s) 31
Cfr13I GGNCC 1 cut(s) 821
Csp6I GTAC 2 cut(s) 346, 1187
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 3 cut(s) 163, 331, 874
CviQI GTAC 2 cut(s) 346, 1187
DdeI CTNAG 5 cut(s) 237, 831, 1047, 1110, 1242
DinI GGCGCC 1 cut(s) 1301
DpnI GATC 3 cut(s) 72, 132, 234
DpnII GATC 3 cut(s) 70, 130, 232
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
EciI GGCGGA 1 cut(s) 1230
Eco130I CCWWGG 2 cut(s) 147, 868
Eco147I AGGCCT 2 cut(s) 836, 1018
Eco31I GGTCTC 2 cut(s) 15, 1151
Eco57I CTGAAG 4 cut(s) 311, 336, 822, 1011
Eco72I CACGTG 1 cut(s) 88
EcoRI GAATTC 3 cut(s) 263, 473, 676
EcoRII CCWGG 3 cut(s) 565, 693, 1162
EcoT14I CCWWGG 2 cut(s) 147, 868
EgeI GGCGCC 1 cut(s) 1301
EheI GGCGCC 1 cut(s) 1301
ErhI CCWWGG 2 cut(s) 147, 868
FaeI CATG 3 cut(s) 166, 334, 877
FatI CATG 3 cut(s) 162, 330, 873
FauNDI CATATG 2 cut(s) 55, 214
Fnu4HI GCNGC 8 cut(s) 203, 307, 731, 1107, 1119, 1122, 1236, 1239
FokI GGATG 5 cut(s) 17, 80, 925, 962, 1217
Fsp4HI GCNGC 8 cut(s) 203, 307, 731, 1107, 1119, 1122, 1236, 1239
FspBI CTAG 2 cut(s) 434, 545
GlaI GCGC 1 cut(s) 1301
GluI GCNGC 8 cut(s) 203, 307, 731, 1107, 1119, 1122, 1236, 1239
GsaI CCCAGC 1 cut(s) 957
GsuI CTGGAG 1 cut(s) 1293
HaeII RGCGCY 1 cut(s) 1303
HaeIII GGCC 4 cut(s) 822, 836, 886, 1018
HapII CCGG 1 cut(s) 32
HhaI GCGC 1 cut(s) 1302
Hin1I GRCGYC 1 cut(s) 1300
Hin1II CATG 3 cut(s) 166, 334, 877
Hin6I GCGC 1 cut(s) 1300
HinP1I GCGC 1 cut(s) 1300
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 4 cut(s) 337, 460, 698, 746
HpaII CCGG 1 cut(s) 32
HphI GGTGA 5 cut(s) 293, 885, 906, 1019, 1220
Hpy166II GTNNAC 2 cut(s) 893, 1061
Hpy188I TCNGA 6 cut(s) 598, 727, 832, 856, 1195, 1245
Hpy188III TCNNGA 7 cut(s) 223, 236, 268, 341, 464, 644, 1029
Hpy8I GTNNAC 2 cut(s) 893, 1061
HpyAV CCTTC 5 cut(s) 84, 220, 550, 656, 1194
HpyCH4IV ACGT 2 cut(s) 87, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 879, 959, 1073, 1115
HpyF3I CTNAG 5 cut(s) 237, 831, 1047, 1110, 1242
HpySE526I ACGT 2 cut(s) 87, 778
Hsp92I GRCGYC 1 cut(s) 1300
Hsp92II CATG 3 cut(s) 166, 334, 877
HspAI GCGC 1 cut(s) 1300
KasI GGCGCC 1 cut(s) 1299
Kzo9I GATC 3 cut(s) 70, 130, 232
LmnI GCTCC 4 cut(s) 40, 1081, 1141, 1217
Lsp1109I GCAGC 5 cut(s) 214, 1105, 1133, 1225, 1247
LweI GCATC 1 cut(s) 1165
MaeI CTAG 2 cut(s) 434, 545
MaeII ACGT 2 cut(s) 87, 778
MalI GATC 3 cut(s) 72, 132, 234
MbiI CCGCTC 1 cut(s) 730
MboI GATC 3 cut(s) 70, 130, 232
MfeI CAATTG 2 cut(s) 500, 1068
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 1063
MluCI AATT 9 cut(s) 181, 263, 315, 473, 485, 500, 670, 676, 1068
Mly113I GGCGCC 1 cut(s) 1300
MlyI GAGTC 1 cut(s) 755
MseI TTAA 5 cut(s) 207, 362, 411, 491, 1151
MspA1I CMGCKG 2 cut(s) 1109, 1238
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 567, 695, 1164
MunI CAATTG 2 cut(s) 500, 1068
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 3 cut(s) 567, 695, 1164
MwoI GCNNNNNNNGC 4 cut(s) 879, 959, 1073, 1115
NarI GGCGCC 1 cut(s) 1300
NdeI CATATG 2 cut(s) 55, 214
NdeII GATC 3 cut(s) 70, 130, 232
NlaIII CATG 3 cut(s) 166, 334, 877
NlaIV GGNNCC 3 cut(s) 36, 132, 1301
NspV TTCGAA 2 cut(s) 23, 47
PaqCI CACCTGC 1 cut(s) 1072
PceI AGGCCT 2 cut(s) 836, 1018
PctI GAATGC 1 cut(s) 31
PfeI GAWTC 3 cut(s) 337, 460, 698
PkrI GCNGC 8 cut(s) 204, 308, 732, 1108, 1120, 1123, 1237, 1240
PleI GAGTC 1 cut(s) 754
PluTI GGCGCC 1 cut(s) 1303
PmaCI CACGTG 1 cut(s) 88
PmlI CACGTG 1 cut(s) 88
PpsI GAGTC 1 cut(s) 754
Ppu21I YACGTR 2 cut(s) 88, 779
PshBI ATTAAT 1 cut(s) 411
Psp6I CCWGG 3 cut(s) 565, 693, 1162
PspCI CACGTG 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 953
PspGI CCWGG 3 cut(s) 565, 693, 1162
PspN4I GGNNCC 3 cut(s) 36, 132, 1301
PspPI GGNCC 1 cut(s) 821
PstI CTGCAG 1 cut(s) 1123
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 1238
RsaI GTAC 2 cut(s) 347, 1188
RsaNI GTAC 2 cut(s) 346, 1187
SaqAI TTAA 5 cut(s) 207, 362, 411, 491, 1151
SatI GCNGC 8 cut(s) 203, 307, 731, 1107, 1119, 1122, 1236, 1239
Sau3AI GATC 3 cut(s) 70, 130, 232
Sau96I GGNCC 1 cut(s) 821
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 3 cut(s) 567, 695, 1164
SduI GDGCHC 1 cut(s) 1063
SfaNI GCATC 1 cut(s) 1165
SfcI CTRYAG 1 cut(s) 1119
SfoI GGCGCC 1 cut(s) 1301
SfuI TTCGAA 2 cut(s) 23, 47
SmlI CTYRAG 5 cut(s) 12, 268, 428, 549, 734
SmoI CTYRAG 5 cut(s) 12, 268, 428, 549, 734
Sse9I AATT 9 cut(s) 181, 263, 315, 473, 485, 500, 670, 676, 1068
SseBI AGGCCT 2 cut(s) 836, 1018
SsiI CCGC 5 cut(s) 304, 307, 730, 1107, 1215
SspDI GGCGCC 1 cut(s) 1299
SspI AATATT 1 cut(s) 298
SspMI CTAG 2 cut(s) 434, 545
StuI AGGCCT 2 cut(s) 836, 1018
StyD4I CCNGG 3 cut(s) 565, 693, 1162
StyI CCWWGG 2 cut(s) 147, 868
TaiI ACGT 2 cut(s) 90, 781
TaqI TCGA 4 cut(s) 23, 47, 188, 902
TasI AATT 9 cut(s) 181, 263, 315, 473, 485, 500, 670, 676, 1068
TatI WGTACW 2 cut(s) 345, 1186
TauI GCSGC 3 cut(s) 309, 733, 1109
TfiI GAWTC 3 cut(s) 337, 460, 698
Tru1I TTAA 5 cut(s) 207, 362, 411, 491, 1151
Tru9I TTAA 5 cut(s) 207, 362, 411, 491, 1151
TscAI CASTG 1 cut(s) 248
TseI GCWGC 5 cut(s) 202, 1118, 1121, 1235, 1238
TspDTI ATGAA 9 cut(s) 308, 402, 497, 510, 890, 923, 927, 941, 1055
TspRI CASTG 1 cut(s) 248
VneI GTGCAC 1 cut(s) 1059
VspI ATTAAT 1 cut(s) 411
XapI RAATTY 4 cut(s) 263, 473, 670, 676
XspI CTAG 2 cut(s) 434, 545
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.