RchiOBHm_Chr2g0131371

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002221
Physical Location & Seq
Reverse (-)
47483003 .. 47486668
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50273

Sequence Viewer

Length: 1314 bp
ATGGATGGGTCTCAAGGTGGTTCGAATGCACCAGCTCCCTTTCTTTCGAAAACATATGACTTGGTGGATGATCCTTCAACCAACCATGTGGTGTCTTGGAGTGAAACTGGTTGTAGTTTTGTAGTCTGGGATCCCACAGAGTTTGCCAAGGATTTGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTTGTCAGGCAGCTTAATACATATGGGTTTAGGAAGGTTGATCCTGAGCAGTGGGAGTTTGCAAATGAGGAATTCGTAAGAGGAGGAAGGCATCTACTGAAGAATATTCACCGTCGCAAGCCAATTCATAGTCACTCCATGCAAAATCACGAGTATTCTTCAATTCCTTTAAGCGAGAAAGAAAGAGAAGAATATGAAAATAAAATCAATAGACTGAATCAGGAGAAGCGCTTGCTCGAGGTAGATCTACAAAGACATCAAAAGGAGAATCAGGAGTTTGAATTGCAAATGCAATTGTTAAATGAACAATTGCAAAAGATGGAACTTCAGCAGAGGCAATATACGACCTTCCTAGCTCAAGTATTGAAGAAACCAGGGTTTGCCTCTTTACTTATGCAACAATCGGAAAATCATAACAAAAAGAGAAGACTGTTGAAACCTAACCTCTTTCCTGATGACTTCAGTATGGAAGGTTTGAATTTGAATTCACAGAAAGAGAACCTGGATTCAGTTTCAACTTCCACATCAAAGTCAGACCGGCTTGAGAAAATGGAGTCTTCCTTAAACTTTTGGGAAGATTTTCTACGTGGTATTGAGGCTATACCTGAAGAAGTAAATGATATTGGCCCTTTGTCTGAGGCATCTCCCATCATTGTTTCAGAAATGCAAGACCTTGGCATGAATAGCAGGCCGTGCTCACCTATATCGAATTTATCTTCGCCGACTTCAATGAATGTTCATTCATCCCCAGAGGCTGGGTCTGCAAATTTTTTCGACATCCTTGCTATATCATCAATGTGTCAAACTGTTGATTTCAGGACTAAATCTTCAGGACTTGATATGAACTCTAAGCCTGATAGTGCACTTGCAGTTGAGGCTCCAAAAGAAAGGGTAGTAGAAATGACAACCGCTGAGCCTGCTGCAGCAAATGATGTCTTTTGGGAGCAATGTCTAACAGAGACCCCCGGTTTAGAAGATGCACAGGAAGTACAATCGGAAAGAAGGGATGGTGATGGCGGAGCGGGTGATACCAATGCAGCTGCTCAGAAAAAGCTTTGGTGGAACACAGATAGTGTAGATAACTTTACAAACCAAATAGGGCGCCTCACTCCAGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

437

Amino Acids

49.59

Weight (kDa)

4.98

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 14 - 103 1.3e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1293
AccB7I CCANNNNNTGG 1 cut(s) 947
AccBSI CCGCTC 1 cut(s) 1214
AciI CCGC 3 cut(s) 1101, 1209, 1214
AclWI GGATC 4 cut(s) 65, 125, 138, 227
AcsI RAATTY 5 cut(s) 263, 670, 676, 901, 958
AcuI CTGAAG 5 cut(s) 311, 503, 637, 819, 1005
AcyI GRCGYC 1 cut(s) 1294
AfaI GTAC 1 cut(s) 1182
AfeI AGCGCT 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 947
AjnI CCWGG 2 cut(s) 565, 693
AluBI AGCT 6 cut(s) 35, 205, 548, 1232, 1246, 1307
AluI AGCT 6 cut(s) 35, 205, 548, 1232, 1246, 1307
Alw21I GWGCWC 2 cut(s) 890, 1057
Alw26I GTCTC 2 cut(s) 15, 1145
Alw44I GTGCAC 1 cut(s) 1053
AlwI GGATC 4 cut(s) 65, 125, 138, 227
AlwNI CAGNNNCTG 1 cut(s) 947
Ama87I CYCGRG 1 cut(s) 428
Aor51HI AGCGCT 1 cut(s) 422
AoxI GGCC 2 cut(s) 817, 881
ApaLI GTGCAC 1 cut(s) 1053
ApeKI GCWGC 5 cut(s) 202, 1112, 1115, 1229, 1232
ApoI RAATTY 5 cut(s) 263, 670, 676, 901, 958
Asp700I GAANNNNTTC 1 cut(s) 771
AspLEI GCGC 2 cut(s) 423, 1296
AspS9I GGNCC 1 cut(s) 818
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 4 cut(s) 293, 882, 1214, 1229
AsuII TTCGAA 2 cut(s) 23, 47
AvaI CYCGRG 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 1057
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 1 cut(s) 1293
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BauI CACGAG 1 cut(s) 341
BbsI GAAGAC 2 cut(s) 625, 741
Bbv12I GWGCWC 2 cut(s) 890, 1057
BbvI GCAGC 5 cut(s) 214, 1099, 1127, 1219, 1241
BccI CCATC 4 cut(s) 505, 848, 1193, 1199
BceAI ACGGC 1 cut(s) 868
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 2 cut(s) 567, 695
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 2 cut(s) 15, 1145
BfaI CTAG 1 cut(s) 545
BfmI CTRYAG 1 cut(s) 1113
BfoI RGCGCY 2 cut(s) 424, 1297
BglII AGATCT 1 cut(s) 436
BisI GCNGC 5 cut(s) 203, 1113, 1116, 1230, 1233
BlpI GCTNAGC 1 cut(s) 1104
BlsI GCNGC 5 cut(s) 204, 1114, 1117, 1231, 1234
Bme1390I CCNGG 3 cut(s) 567, 695, 1158
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 3 cut(s) 132, 1071, 1295
BmrFI CCNGG 3 cut(s) 567, 695, 1158
BmsI GCATC 3 cut(s) 292, 842, 1159
BpiI GAAGAC 2 cut(s) 625, 741
BpmI CTGGAG 1 cut(s) 1287
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 1104
Bpu14I TTCGAA 2 cut(s) 23, 47
BpuEI CTTGAG 2 cut(s) 534, 755
BpuMI CCSGG 1 cut(s) 1158
BsaAI YACGTR 1 cut(s) 779
BsaHI GRCGYC 1 cut(s) 1294
BsaI GGTCTC 2 cut(s) 15, 1145
BsaJI CCNNGG 4 cut(s) 147, 566, 865, 1156
Bsc4I CCNNNNNNNGG 1 cut(s) 947
Bse118I RCCGGY 1 cut(s) 729
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 1145
BseBI CCWGG 2 cut(s) 567, 695
BseDI CCNNGG 4 cut(s) 147, 566, 865, 1156
BseGI GGATG 5 cut(s) 10, 73, 935, 969, 1204
BseLI CCNNNNNNNGG 1 cut(s) 947
BseMI GCAATG 1 cut(s) 1145
BseMII CTCAG 4 cut(s) 228, 819, 1095, 1250
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 1057
BseXI GCAGC 5 cut(s) 214, 1099, 1127, 1219, 1241
BseYI CCCAGC 1 cut(s) 947
BshFI GGCC 2 cut(s) 819, 883
BshNI GGYRCC 1 cut(s) 1293
BsiHKAI GWGCWC 2 cut(s) 890, 1057
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 2 cut(s) 730, 1158
BslI CCNNNNNNNGG 1 cut(s) 947
BsmAI GTCTC 2 cut(s) 15, 1145
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 2 cut(s) 819, 883
Bso31I GGTCTC 2 cut(s) 15, 1145
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 2 cut(s) 23, 47
Bsp1286I GDGCHC 2 cut(s) 890, 1057
Bsp143I GATC 4 cut(s) 70, 130, 232, 436
Bsp1720I GCTNAGC 1 cut(s) 1104
BspACI CCGC 3 cut(s) 1101, 1209, 1214
BspANI GGCC 2 cut(s) 819, 883
BspCNI CTCAG 4 cut(s) 229, 820, 1096, 1249
BspLI GGNNCC 3 cut(s) 132, 1071, 1295
BspMAI CTGCAG 1 cut(s) 1117
BspPI GGATC 4 cut(s) 65, 125, 138, 227
BspT104I TTCGAA 2 cut(s) 23, 47
BspT107I GGYRCC 1 cut(s) 1293
BspTNI GGTCTC 2 cut(s) 15, 1145
BsrBI CCGCTC 1 cut(s) 1214
BsrDI GCAATG 1 cut(s) 1145
BsrFI RCCGGY 1 cut(s) 729
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 4 cut(s) 147, 566, 865, 1156
BssMI GATC 4 cut(s) 70, 130, 232, 436
BssNI GRCGYC 1 cut(s) 1294
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 2 cut(s) 147, 865
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 2 cut(s) 567, 695
Bst4CI ACNGT 3 cut(s) 305, 624, 1000
BstACI GRCGYC 1 cut(s) 1294
BstAPI GCANNNNNTGC 1 cut(s) 885
BstBAI YACGTR 1 cut(s) 779
BstBI TTCGAA 2 cut(s) 23, 47
BstC8I GCNNGC 4 cut(s) 311, 425, 881, 1110
BstDEI CTNAG 5 cut(s) 237, 828, 1041, 1104, 1236
BstF5I GGATG 5 cut(s) 10, 73, 935, 969, 1204
BstH2I RGCGCY 2 cut(s) 424, 1297
BstHHI GCGC 2 cut(s) 423, 1296
BstKTI GATC 4 cut(s) 73, 133, 235, 439
BstMAI GTCTC 2 cut(s) 15, 1145
BstMBI GATC 4 cut(s) 70, 130, 232, 436
BstMWI GCNNNNNNNGC 5 cut(s) 876, 885, 953, 1067, 1109
BstNI CCWGG 2 cut(s) 567, 695
BstSCI CCNGG 3 cut(s) 565, 693, 1156
BstSFI CTRYAG 1 cut(s) 1113
BstSLI GKGCMC 1 cut(s) 1057
BstV1I GCAGC 5 cut(s) 214, 1099, 1127, 1219, 1241
BstV2I GAAGAC 2 cut(s) 625, 741
BstX2I RGATCY 2 cut(s) 130, 436
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 2 cut(s) 130, 436
BsuRI GGCC 2 cut(s) 819, 883
BtsCI GGATG 5 cut(s) 10, 73, 935, 969, 1204
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 4 cut(s) 311, 425, 881, 1110
CaiI CAGNNNCTG 1 cut(s) 947
CfoI GCGC 2 cut(s) 423, 1296
Cfr10I RCCGGY 1 cut(s) 729
Cfr13I GGNCC 1 cut(s) 818
Csp6I GTAC 1 cut(s) 1181
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 4 cut(s) 86, 163, 331, 871
CviQI GTAC 1 cut(s) 1181
DdeI CTNAG 5 cut(s) 237, 828, 1041, 1104, 1236
DinI GGCGCC 1 cut(s) 1295
DpnI GATC 4 cut(s) 72, 132, 234, 438
DpnII GATC 4 cut(s) 70, 130, 232, 436
EciI GGCGGA 1 cut(s) 1224
Eco130I CCWWGG 2 cut(s) 147, 865
Eco31I GGTCTC 2 cut(s) 15, 1145
Eco47III AGCGCT 1 cut(s) 422
Eco57I CTGAAG 5 cut(s) 311, 503, 637, 819, 1005
Eco88I CYCGRG 1 cut(s) 428
EcoRI GAATTC 2 cut(s) 263, 676
EcoRII CCWGG 2 cut(s) 565, 693
EcoT14I CCWWGG 2 cut(s) 147, 865
EgeI GGCGCC 1 cut(s) 1295
EheI GGCGCC 1 cut(s) 1295
ErhI CCWWGG 2 cut(s) 147, 865
FaeI CATG 4 cut(s) 89, 166, 334, 874
FatI CATG 4 cut(s) 85, 162, 330, 870
FauI CCCGC 1 cut(s) 1207
FauNDI CATATG 2 cut(s) 55, 214
Fnu4HI GCNGC 5 cut(s) 203, 1113, 1116, 1230, 1233
FokI GGATG 5 cut(s) 17, 80, 922, 956, 1211
Fsp4HI GCNGC 5 cut(s) 203, 1113, 1116, 1230, 1233
FspBI CTAG 1 cut(s) 545
GlaI GCGC 2 cut(s) 422, 1295
GluI GCNGC 5 cut(s) 203, 1113, 1116, 1230, 1233
GsaI CCCAGC 1 cut(s) 951
GsuI CTGGAG 1 cut(s) 1287
HaeII RGCGCY 2 cut(s) 424, 1297
HaeIII GGCC 2 cut(s) 819, 883
HapII CCGG 2 cut(s) 730, 1158
HhaI GCGC 2 cut(s) 423, 1296
Hin1I GRCGYC 1 cut(s) 1294
Hin1II CATG 4 cut(s) 89, 166, 334, 874
Hin6I GCGC 2 cut(s) 421, 1294
HinP1I GCGC 2 cut(s) 421, 1294
HindIII AAGCTT 1 cut(s) 1244
HinfI GANTC 4 cut(s) 409, 460, 698, 746
HpaII CCGG 2 cut(s) 730, 1158
HphI GGTGA 4 cut(s) 293, 882, 1214, 1229
Hpy166II GTNNAC 1 cut(s) 1055
Hpy188I TCNGA 6 cut(s) 598, 727, 829, 853, 1189, 1239
Hpy188III TCNNGA 7 cut(s) 236, 341, 413, 464, 644, 1009, 1023
Hpy8I GTNNAC 1 cut(s) 1055
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 6 cut(s) 84, 220, 273, 550, 656, 1188
HpyCH4III ACNGT 3 cut(s) 305, 624, 1000
HpyCH4IV ACGT 1 cut(s) 778
HpyF10VI GCNNNNNNNGC 5 cut(s) 876, 885, 953, 1067, 1109
HpyF3I CTNAG 5 cut(s) 237, 828, 1041, 1104, 1236
HpySE526I ACGT 1 cut(s) 778
Hsp92I GRCGYC 1 cut(s) 1294
Hsp92II CATG 4 cut(s) 89, 166, 334, 874
HspAI GCGC 2 cut(s) 421, 1294
KasI GGCGCC 1 cut(s) 1293
Kzo9I GATC 4 cut(s) 70, 130, 232, 436
LmnI GCTCC 4 cut(s) 40, 1075, 1135, 1211
Lsp1109I GCAGC 5 cut(s) 214, 1099, 1127, 1219, 1241
LweI GCATC 3 cut(s) 292, 842, 1159
MaeI CTAG 1 cut(s) 545
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 1 cut(s) 323
MalI GATC 4 cut(s) 72, 132, 234, 438
MbiI CCGCTC 1 cut(s) 1214
MboI GATC 4 cut(s) 70, 130, 232, 436
MfeI CAATTG 2 cut(s) 485, 500
MflI RGATCY 2 cut(s) 130, 436
MhlI GDGCHC 2 cut(s) 890, 1057
Mly113I GGCGCC 1 cut(s) 1294
MlyI GAGTC 1 cut(s) 755
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 4 cut(s) 207, 362, 491, 755
MslI CAYNNNNRTG 1 cut(s) 988
MspA1I CMGCKG 2 cut(s) 1103, 1232
MspI CCGG 2 cut(s) 730, 1158
MspR9I CCNGG 3 cut(s) 567, 695, 1158
MunI CAATTG 2 cut(s) 485, 500
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 2 cut(s) 567, 695
MwoI GCNNNNNNNGC 5 cut(s) 876, 885, 953, 1067, 1109
NarI GGCGCC 1 cut(s) 1294
NciI CCSGG 1 cut(s) 1158
NdeI CATATG 2 cut(s) 55, 214
NdeII GATC 4 cut(s) 70, 130, 232, 436
NlaIII CATG 4 cut(s) 89, 166, 334, 874
NlaIV GGNNCC 3 cut(s) 132, 1071, 1295
NmuCI GTSAC 1 cut(s) 323
NspV TTCGAA 2 cut(s) 23, 47
PaeR7I CTCGAG 1 cut(s) 428
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 3 cut(s) 409, 460, 698
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 5 cut(s) 204, 1114, 1117, 1231, 1234
PleI GAGTC 1 cut(s) 754
PluTI GGCGCC 1 cut(s) 1297
PpsI GAGTC 1 cut(s) 754
Ppu21I YACGTR 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 565, 693
PspFI CCCAGC 1 cut(s) 947
PspGI CCWGG 2 cut(s) 565, 693
PspN4I GGNNCC 3 cut(s) 132, 1071, 1295
PspPI GGNCC 1 cut(s) 818
PspXI VCTCGAGB 1 cut(s) 428
PstI CTGCAG 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 947
PsuI RGATCY 2 cut(s) 130, 436
PvuII CAGCTG 1 cut(s) 1232
RsaI GTAC 1 cut(s) 1182
RsaNI GTAC 1 cut(s) 1181
RseI CAYNNNNRTG 1 cut(s) 988
SaqAI TTAA 4 cut(s) 207, 362, 491, 755
SatI GCNGC 5 cut(s) 203, 1113, 1116, 1230, 1233
Sau3AI GATC 4 cut(s) 70, 130, 232, 436
Sau96I GGNCC 1 cut(s) 818
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 3 cut(s) 567, 695, 1158
SduI GDGCHC 2 cut(s) 890, 1057
SfaNI GCATC 3 cut(s) 292, 842, 1159
SfcI CTRYAG 1 cut(s) 1113
SfoI GGCGCC 1 cut(s) 1295
Sfr274I CTCGAG 1 cut(s) 428
SfuI TTCGAA 2 cut(s) 23, 47
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 988
SmlI CTYRAG 4 cut(s) 12, 428, 549, 734
SmoI CTYRAG 4 cut(s) 12, 428, 549, 734
SsiI CCGC 3 cut(s) 1101, 1209, 1214
SspDI GGCGCC 1 cut(s) 1293
SspI AATATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 3 cut(s) 565, 693, 1156
StyI CCWWGG 2 cut(s) 147, 865
TaaI ACNGT 3 cut(s) 305, 624, 1000
TaiI ACGT 1 cut(s) 781
TaqI TCGA 6 cut(s) 23, 47, 188, 429, 899, 966
TatI WGTACW 1 cut(s) 1180
TfiI GAWTC 3 cut(s) 409, 460, 698
Tru1I TTAA 4 cut(s) 207, 362, 491, 755
Tru9I TTAA 4 cut(s) 207, 362, 491, 755
TscAI CASTG 1 cut(s) 248
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 5 cut(s) 202, 1112, 1115, 1229, 1232
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 8 cut(s) 308, 402, 510, 887, 920, 924, 938, 1049
TspRI CASTG 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 947
VneI GTGCAC 1 cut(s) 1053
XapI RAATTY 5 cut(s) 263, 670, 676, 901, 958
XhoI CTCGAG 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.